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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
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                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
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                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <consortium name="Ensembl"/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Chapman J."/>
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                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
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                <person name="Doyle S."/>
                <person name="Francino P."/>
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                <person name="Haga S."/>
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                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
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                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
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                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01002997">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6PT87"/>
    <dbReference type="Ensembl" id="ENSCINT00000024128.2">
        <property type="protein sequence ID" value="ENSCINP00000023882.2"/>
        <property type="gene ID" value="ENSCING00000006538.3"/>
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        <property type="component" value="Chromosome 9"/>
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        <location>
            <begin position="71"/>
            <end position="177"/>
        </location>
    </feature>
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            <begin position="72"/>
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        </location>
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            <begin position="110"/>
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            <begin position="134"/>
            <end position="148"/>
        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="149"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000023882.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="206" mass="23588" checksum="2E22875CBEDD7B71" modified="2012-04-18" version="2">MSVGTTNENKYVGIYDENTMHTQVYSPVPEKYIKSKKSKAFGMTLRPRIPLKQLNAVSSIARVYLTTKHKRLESESDSERPTPQDSKLKRKMQTTFNFVAQKITGKQKRQRLESNQKVNEKTPRTQYRERMNFSPLTPNNPFDSPPTNSCSHSLRSSVSTSSSNKQKTPISRKRSYRADDFNNHLEQVSCGIQQLQANSDNLVTVI</sequence>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Alpha-L-fucosidase is responsible for hydrolyzing the alpha-1,6-linked fucose joined to the reducing-end N-acetylglucosamine of the carbohydrate moieties of glycoproteins.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the glycosyl hydrolase 29 family.</text>
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        <property type="gene ID" value="ENSCING00000005740.3"/>
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        <property type="evidence" value="ECO:0000318"/>
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        <property type="term" value="P:fucose metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016139">
        <property type="term" value="P:glycoside catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
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        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016286">
        <property type="entry name" value="FUC_metazoa-typ"/>
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        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000933">
        <property type="entry name" value="Glyco_hydro_29"/>
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    <dbReference type="InterPro" id="IPR018526">
        <property type="entry name" value="Glyco_hydro_29_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057739">
        <property type="entry name" value="Glyco_hydro_29_N"/>
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    <dbReference type="PANTHER" id="PTHR10030">
        <property type="entry name" value="ALPHA-L-FUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10030:SF37">
        <property type="entry name" value="ALPHA-L-FUCOSIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01120">
        <property type="entry name" value="Alpha_L_fucos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001092">
        <property type="entry name" value="Alpha-L-fucosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00741">
        <property type="entry name" value="GLHYDRLASE29"/>
    </dbReference>
    <dbReference type="SMART" id="SM00812">
        <property type="entry name" value="Alpha_L_fucos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00385">
        <property type="entry name" value="ALPHA_L_FUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="7 8" id="KW-0326">Glycosidase</keyword>
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    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
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            <begin position="20"/>
            <end position="406"/>
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            <begin position="47"/>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
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    <name>F6Q909_CIOIN</name>
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        <submittedName>
            <fullName evidence="16">Uncharacterized protein</fullName>
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        <name type="common">Transparent sea squirt</name>
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    <reference evidence="17" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
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                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">wild type</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="EAAA01000560">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="F6RGU6"/>
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    <dbReference type="GeneTree" id="ENSGT00890000139676"/>
    <dbReference type="HOGENOM" id="CLU_002753_3_1_1"/>
    <dbReference type="InParanoid" id="F6RGU6"/>
    <dbReference type="OMA" id="SNCANIE"/>
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        <property type="component" value="Chromosome 10"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:cell surface receptor signaling pathway"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15040">
        <property type="entry name" value="7tmB2_Adhesion"/>
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        <property type="entry name" value="GG11888"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.220.50:FF:000058">
        <property type="entry name" value="Uncharacterized protein"/>
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        <property type="entry name" value="GPS"/>
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    <dbReference type="PANTHER" id="PTHR45692:SF1">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 2 PROFILE 2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F2_4 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="7tm_2"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="GAIN_B"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="109"/>
            <end position="135"/>
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    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="8">
        <location>
            <begin position="111"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>F6S6C5</accession>
    <name>F6S6C5_CIOIN</name>
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        <recommendedName>
            <fullName evidence="9">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">wild type</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="EAAA01001865">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6S6C5"/>
    <dbReference type="STRING" id="7719.ENSCINP00000011317"/>
    <dbReference type="Ensembl" id="ENSCINT00000011317.3">
        <property type="protein sequence ID" value="ENSCINP00000011317.3"/>
        <property type="gene ID" value="ENSCING00000005483.3"/>
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    <dbReference type="GeneTree" id="ENSGT00940000169211"/>
    <dbReference type="HOGENOM" id="CLU_602091_0_0_1"/>
    <dbReference type="InParanoid" id="F6S6C5"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042800">
        <property type="term" value="F:histone H3K4 methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12304">
        <property type="entry name" value="RRM_Set1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
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    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044570">
        <property type="entry name" value="Set1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45814">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE SETD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45814:SF2">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE SETD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
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    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RRM" evidence="9">
        <location>
            <begin position="86"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="191"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="339"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="191"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="265"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="290"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="306"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="364"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="404"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="437"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000011317.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="455" mass="51791" checksum="3C50F92D65D8DCC9" modified="2011-07-27" version="1">MKKQQPAAAFRGNFKVIVDPQLKKGQEKLVRFNGTEYSCENHGQAPVAVRDPRARVSRLWSRKERIDLPVPRYKVDENYTGDVPPREVTFSKLNDNITKQFMEQMCSKYGKLERVRVYHHPKTNKHMGLAKVVFASIKSASMCISNLHQTSIMGNIINAEIDSKGELLKHLFNKIIRGEILAETMPDVIRHTQEKPITRPVPVKDRRKSSQEDPRRRSSSDRKSSMEQTTSSHGPKTPPSTLIDGKPNDNKQDLLNMEGARVPMHSNTKSSDNESGVSDEATRQHQNPRVNKRKVKKKKKSRKYSRNSDSDTEMEKGKKGLPMWSTELEQRIENLLSEQNRKYNLDSTSRSSSHEFKSTSSTKSSDRKYSEQSGTDNDHSETAEESDVFDQASMSNHNHVETAELIDLEDGECSGDAEEVHNGRSRKQSPVIQVMERAQHETSPDTKSERVSYSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>F6S6V5</accession>
    <accession>A0A1W5BF73</accession>
    <name>F6S6V5_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">ADP-ribosylation factor-like protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100176430</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_018670963.1">
        <property type="nucleotide sequence ID" value="XM_018815418.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6S6V5"/>
    <dbReference type="FunCoup" id="F6S6V5">
        <property type="interactions" value="24"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000028182"/>
    <dbReference type="Ensembl" id="ENSCINT00000028428.2">
        <property type="protein sequence ID" value="ENSCINP00000028182.2"/>
        <property type="gene ID" value="ENSCING00000016182.2"/>
    </dbReference>
    <dbReference type="GeneID" id="100176430"/>
    <dbReference type="KEGG" id="cin:100176430"/>
    <dbReference type="GeneTree" id="ENSGT00940000155737"/>
    <dbReference type="HOGENOM" id="CLU_040729_12_0_1"/>
    <dbReference type="InParanoid" id="F6S6V5"/>
    <dbReference type="OMA" id="EGMEWVC"/>
    <dbReference type="OrthoDB" id="2011769at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015630">
        <property type="term" value="C:microtubule cytoskeleton"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04155">
        <property type="entry name" value="Arl3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000281">
        <property type="entry name" value="ADP-ribosylation factor-like protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044612">
        <property type="entry name" value="ARL2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45697">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-LIKE PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00328">
        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 12 14" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0519">Myristate</keyword>
    <keyword evidence="6 12 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="24"/>
            <end position="31"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="126"/>
            <end position="129"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00040616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000028182.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="182" mass="20399" checksum="66C0979F0F79E7AB" modified="2011-07-27" version="1">MGLLKLLRKLKSESNKELRILLLGLDNAGKTTILKKLASEDINHITPTQGFNIKSVQSQGFKLNVWDIGGQRRIRPYWRNYFENTDVLIYVVDSTDKKRLEETGVELEELVTDEKLSGVPLLVFANKQDLLQAAPASDIADGLSLHTLSGRTYQIQACSALKGEGIQDGLEWVMKTVSKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>F6SCX5</accession>
    <name>F6SCX5_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">receptor protein serine/threonine kinase</fullName>
            <ecNumber evidence="5">2.7.11.30</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">tgfbr-ic</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.30" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="F6SCX5"/>
    <dbReference type="FunCoup" id="F6SCX5">
        <property type="interactions" value="95"/>
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    <dbReference type="STRING" id="7719.ENSCINP00000005916"/>
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        <property type="protein sequence ID" value="ENSCINP00000005916.3"/>
        <property type="gene ID" value="ENSCING00000014075.2"/>
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    <dbReference type="GeneTree" id="ENSGT00940000168401"/>
    <dbReference type="HOGENOM" id="CLU_000288_8_1_1"/>
    <dbReference type="InParanoid" id="F6SCX5"/>
    <dbReference type="OMA" id="RDMIEMT"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048179">
        <property type="term" value="C:activin receptor complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048185">
        <property type="term" value="F:activin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016361">
        <property type="term" value="F:activin receptor activity, type I"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032924">
        <property type="term" value="P:activin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071363">
        <property type="term" value="P:cellular response to growth factor stimulus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14143">
        <property type="entry name" value="STKc_TGFbR1_ACVR1b_ACVR1c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000018">
        <property type="entry name" value="Receptor protein serine/threonine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Receptor protein serine/threonine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.60.10">
        <property type="entry name" value="CD59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000472">
        <property type="entry name" value="Activin_recp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003605">
        <property type="entry name" value="GS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045860">
        <property type="entry name" value="Snake_toxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000333">
        <property type="entry name" value="TGFB_receptor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23255:SF71">
        <property type="entry name" value="RECEPTOR PROTEIN SERINE_THREONINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23255">
        <property type="entry name" value="TRANSFORMING GROWTH FACTOR-BETA RECEPTOR TYPE I AND II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01064">
        <property type="entry name" value="Activin_recp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08515">
        <property type="entry name" value="TGF_beta_GS"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="GS"/>
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    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57302">
        <property type="entry name" value="Snake toxin-like"/>
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    </dbReference>
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        <property type="entry name" value="GS"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 18 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="16 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 18 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-0675">Receptor</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 19" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="111"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="GS" evidence="22">
        <location>
            <begin position="155"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="21">
        <location>
            <begin position="187"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
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            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
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            <dbReference type="ARBA" id="ARBA00022842"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS51256"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000005916.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="486" mass="55006" checksum="E7DAAD3CD233608A" modified="2012-04-18" version="1">SALTCYCGGSRENPYCERNVFNCTVPEGVCYAMTEVLPISKNILTQRGCFRKNRLVPYNRPFVCEPVNKSNHQVICCKTDFCNHPEAMEAPTLPTTTASIQTNQDQLSTTLIVLLSVGGFLLMAMLVLVAVFYKKQHSKRNDPLVESQNSSLPDTNMQDLLDDKDFSTTGSGSGLPLLVHRTIARQIQLQEVIGKGRYGEVFRGKWRGEDVAVKKFVTRDERSWAREAEIYQTIMLRHENILGFIAADNKDVGTWTELWLISEYHENGSLFDYLGETRVDVGAMLKLCSSIASGLAHLHNEIVGTLGKPPIAHCDLKSKNILVKKNGECAIADLGLAVRHDSSTDTIDIPPNNRVGTKRYMAPEILDDTLNMLHFDSFKRADIYSLGLVLWEIGRRCSIGGQNLDYQLPYYNMVPDDPTIEQMRVVVCEKMFRPQKEATWHKYDTMERMWKLMNECWYESGCARLTALRVKKTIAKIEDEEGVKNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>F6SUT6</accession>
    <name>F6SUT6_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">A disintegrin and metalloproteinase with thrombospondin motifs 6</fullName>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">wild type</strain>
        </source>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="14">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
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</entry>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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            <authorList>
                <consortium name="Ensembl"/>
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    <dbReference type="InterPro" id="IPR057954">
        <property type="entry name" value="SET_TTL12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004344">
        <property type="entry name" value="TTL/TTLL_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027749">
        <property type="entry name" value="TTLL12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46088">
        <property type="entry name" value="TUBULIN--TYROSINE LIGASE-LIKE PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46088:SF1">
        <property type="entry name" value="TUBULIN--TYROSINE LIGASE-LIKE PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25556">
        <property type="entry name" value="SET_TTL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03133">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51221">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin--tyrosine ligase-like protein 12 SET-like" evidence="1">
        <location>
            <begin position="61"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin--tyrosine ligase-like protein 12 SET-like" evidence="1">
        <location>
            <begin position="134"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF25556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000002350.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="684" mass="79805" checksum="66905B35108410AA" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>F6TI75</accession>
    <name>F6TI75_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GBD/FH3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="F6TI75"/>
    <dbReference type="STRING" id="7719.ENSCINP00000002915"/>
    <dbReference type="Ensembl" id="ENSCINT00000002915.3">
        <property type="protein sequence ID" value="ENSCINP00000002915.3"/>
        <property type="gene ID" value="ENSCING00000001473.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154807"/>
    <dbReference type="HOGENOM" id="CLU_027716_0_0_1"/>
    <dbReference type="InParanoid" id="F6TI75"/>
    <dbReference type="OMA" id="KERMLKW"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000056">
        <property type="entry name" value="FH1/FH2 domain-containing protein 3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056771">
        <property type="entry name" value="FH3_FHOD1-3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041387">
        <property type="entry name" value="FHOD1_GBD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014768">
        <property type="entry name" value="GBD/FH3_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45920">
        <property type="entry name" value="FORMIN HOMOLOGY 2 DOMAIN CONTAINING, ISOFORM I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45920:SF4">
        <property type="entry name" value="FORMIN HOMOLOGY 2 DOMAIN CONTAINING, ISOFORM I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24959">
        <property type="entry name" value="FH3_FHOD1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18382">
        <property type="entry name" value="Formin_GBD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51232">
        <property type="entry name" value="GBD_FH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GBD/FH3" evidence="3">
        <location>
            <begin position="54"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="336"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="502"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="378"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="502"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000002915.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="554" mass="63897" checksum="5222DF939D97DBA8" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>F6TJ76</accession>
    <name>F6TJ76_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Proteasome subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC113474124</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">wild type</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the proteasome, a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the proteasome complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase T1B family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001711">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_026689629.1">
        <property type="nucleotide sequence ID" value="XM_026833828.1"/>
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    <dbReference type="AlphaFoldDB" id="F6TJ76"/>
    <dbReference type="FunCoup" id="F6TJ76">
        <property type="interactions" value="1178"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000019473"/>
    <dbReference type="Ensembl" id="ENSCINT00000019473.3">
        <property type="protein sequence ID" value="ENSCINP00000019473.3"/>
        <property type="gene ID" value="ENSCING00000009575.3"/>
    </dbReference>
    <dbReference type="GeneID" id="113474124"/>
    <dbReference type="KEGG" id="cin:113474124"/>
    <dbReference type="GeneTree" id="ENSGT00550000074820"/>
    <dbReference type="HOGENOM" id="CLU_035750_10_0_1"/>
    <dbReference type="InParanoid" id="F6TJ76"/>
    <dbReference type="OMA" id="CSEQLYG"/>
    <dbReference type="OrthoDB" id="204949at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019774">
        <property type="term" value="C:proteasome core complex, beta-subunit complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03759">
        <property type="entry name" value="proteasome_beta_type_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000003">
        <property type="entry name" value="Proteasome subunit beta type-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033811">
        <property type="entry name" value="Proteasome_beta_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
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                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
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                <person name="Wang H.G."/>
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                <person name="Azumi K."/>
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                <person name="Kobayashi K."/>
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                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
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                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
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                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
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                <person name="Putnam N."/>
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                <person name="Terry A."/>
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                <person name="Wang H.G."/>
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                <person name="Azumi K."/>
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                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
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                <person name="Hyatt P.D."/>
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                <person name="Richardson P."/>
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                <person name="Levine M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01000250">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6TTB4"/>
    <dbReference type="Ensembl" id="ENSCINT00000008853.3">
        <property type="protein sequence ID" value="ENSCINP00000008853.3"/>
        <property type="gene ID" value="ENSCING00000004297.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000169202"/>
    <dbReference type="HOGENOM" id="CLU_072960_0_0_1"/>
    <dbReference type="InParanoid" id="F6TTB4"/>
    <dbReference type="OMA" id="YECAREV"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00037">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050111">
        <property type="entry name" value="C-type_lectin/snaclec_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22803">
        <property type="entry name" value="MANNOSE, PHOSPHOLIPASE, LECTIN RECEPTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="185"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="2">
        <location>
            <begin position="20"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000008853.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="330" mass="37451" checksum="103CBD191A9E0586" modified="2012-04-18" version="2">LLLTNFFLQNYTMNLLRRHTDDICYKYLNKTWGELHYHRKMVDFDEAENHCKQVDRSELAIIKDQATLDRLIINAPLTNGCFDQYYWIGLRKEGFRFVWRNGDKYNKSFINVDNNWNGNCVSLLRERGSSAIRFMPQACNVALSFVCFTPRAPKPKVVEGIPTPIQTIVNETTTTQHSSIPPATIGLIAALLALLLLTVILVVVCIRSKKQTRSNRGKEVPKKVAMDKQVVLSNEFALYSSIDVENQYESAHEINPIYNMDITPAPTNVCMQNNQDKQQTPDDVNIVSASDNDVTSSEFDSANTPDATYAVVNKKTKQVTDVYSVVNKNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>F6TUV6</accession>
    <accession>A0A1W2WDW3</accession>
    <name>F6TUV6_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cyclin-H</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100177816</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Regulates CDK7, the catalytic subunit of the CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Associates primarily with CDK7 and MAT1 to form the CAK complex. CAK can further associate with the core-TFIIH to form the TFIIH basal transcription factor.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclin family. Cyclin C subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002129501.1">
        <property type="nucleotide sequence ID" value="XM_002129465.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6TUV6"/>
    <dbReference type="FunCoup" id="F6TUV6">
        <property type="interactions" value="371"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000010895"/>
    <dbReference type="Ensembl" id="ENSCINT00000010895.3">
        <property type="protein sequence ID" value="ENSCINP00000010895.3"/>
        <property type="gene ID" value="ENSCING00000005288.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100177816"/>
    <dbReference type="KEGG" id="cin:100177816"/>
    <dbReference type="GeneTree" id="ENSGT00940000168613"/>
    <dbReference type="HOGENOM" id="CLU_022620_0_0_1"/>
    <dbReference type="InParanoid" id="F6TUV6"/>
    <dbReference type="OMA" id="FRVEQNT"/>
    <dbReference type="OrthoDB" id="340962at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070985">
        <property type="term" value="C:transcription factor TFIIK complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd20524">
        <property type="entry name" value="CYCLIN_CCNH_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20525">
        <property type="entry name" value="CYCLIN_CCNH_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000029">
        <property type="entry name" value="Cyclin h"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043198">
        <property type="entry name" value="Cyclin/Ssn8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031658">
        <property type="entry name" value="Cyclin_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027081">
        <property type="entry name" value="CyclinH/Ccl1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00569">
        <property type="entry name" value="ccl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10026">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16899">
        <property type="entry name" value="Cyclin_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3 7" id="KW-0195">Cyclin</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="8">
        <location>
            <begin position="64"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00026042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000010895.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="329" mass="38532" checksum="63BC0471EAEC5B52" modified="2012-04-18" version="2">MFNSSTQLKNWTFEHEEELIKLRNNANTKYRTSYCDVNNHVTPDADTFFLTVSEHCFLVQHYERKLQEICWKFKPPMPLNVVGTSCMYLKRLNLRKSVMDYHPRLMHLACIWLACKTEEFNISMDQFVQQVAHGNEEIGDAILTIELILIQELNFHLTIHNPFRPLEGFLIDLKTRYRNLENAEQLRKPAKDFLVRSLNTDVGLLYAPSQLALAALLSAASMRNLNIDRYVTSVLLVGQPQTVLEQTIQRIKKIRTIVKNAVSAPLPSDQIEILEQKLSHCYNKEKDPTSYEYSCLQQEIESQKYDKQTKKRKLEHERRRKIETELLGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>F6TYZ7</accession>
    <name>F6TYZ7_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Galactosylceramide sulfotransferase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the galactose-3-O-sulfotransferase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="F6TYZ7"/>
    <dbReference type="STRING" id="7719.ENSCINP00000025980"/>
    <dbReference type="Ensembl" id="ENSCINT00000026226.2">
        <property type="protein sequence ID" value="ENSCINP00000025980.2"/>
        <property type="gene ID" value="ENSCING00000014344.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182923"/>
    <dbReference type="HOGENOM" id="CLU_1510082_0_0_1"/>
    <dbReference type="InParanoid" id="F6TYZ7"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001733">
        <property type="term" value="F:galactosylceramide sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009247">
        <property type="term" value="P:glycolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009729">
        <property type="entry name" value="Gal-3-0_sulfotransfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14647">
        <property type="entry name" value="GALACTOSE-3-O-SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14647:SF87">
        <property type="entry name" value="PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06990">
        <property type="entry name" value="Gal-3-0_sulfotr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000025980.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="178" mass="20764" checksum="C16EDEDD385BFE7E" modified="2012-04-18" version="2">MNEQAKYLKLAAERIEQEFDLVMISDHFMESLILLKDLMCWDLKDVVSLKLNSRKSKSPEQPEQLRRKIRNWNKADAFLFDHFNATLWRKVEAFGHQRMAQEIAELKRLNGEFFKECVNDTGVVGGKNKLFNPNGRLDLKAYAPKNNVSQMCQLATMGTLQISAKLRDNYLRQKQLLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>F6TZ58</accession>
    <name>F6TZ58_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="F6TZ58"/>
    <dbReference type="Ensembl" id="ENSCINT00000026225.2">
        <property type="protein sequence ID" value="ENSCINP00000025979.2"/>
        <property type="gene ID" value="ENSCING00000014343.2"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1590127_0_0_1"/>
    <dbReference type="InParanoid" id="F6TZ58"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042923">
        <property type="term" value="F:neuropeptide binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007218">
        <property type="term" value="P:neuropeptide signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24229">
        <property type="entry name" value="NEUROPEPTIDES RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24229:SF109">
        <property type="entry name" value="SOMATOSTATIN RECEPTOR TYPE 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="59"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000025979.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="168" mass="19743" checksum="949C4D5701748010" modified="2012-04-18" version="2">MLEDNNGGWKYPEWMCKGKETILFLNYYASILFLTIMALDRYVAVCKSFSPLFRNLREIRVAVVASIVIWIICLLMTIPVIKYSHKVGVKGNCTCGYKFPHFNLNEDLLPFSGDEKHCNNPEEILQNYDYEATDLYLHMNNESHYDVTIENHYDVTMNDVDLLHCHYW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>F6U4I8</accession>
    <name>F6U4I8_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
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        <scope>IDENTIFICATION</scope>
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    </evidence>
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        <source>
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</entry>
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        <name type="scientific">Ciona intestinalis</name>
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    <reference evidence="14" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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    <reference evidence="13" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
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                <person name="Inaba K."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="13">wild type</strain>
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    <reference evidence="13" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="13" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
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        <text evidence="2">Belongs to the RRN7/TAF1B family.</text>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Rrn7_cyclin_C"/>
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        <source>
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        </source>
    </evidence>
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        <source>
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        <source>
            <dbReference type="Pfam" id="PF20645"/>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000011299.3"/>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    </evidence>
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</entry>
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        <recommendedName>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
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                <person name="Wasserscheid J."/>
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                <person name="Roe B.A."/>
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    <sequence length="515" mass="59997" checksum="BC911819EA11F8AB" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>F6URF6</accession>
    <name>F6URF6_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Transcription factor protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">tef/hlf</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bZIP family. PAR subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="F6URF6"/>
    <dbReference type="IntAct" id="F6URF6">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000004693"/>
    <dbReference type="Ensembl" id="ENSCINT00000004693.3">
        <property type="protein sequence ID" value="ENSCINP00000004693.3"/>
        <property type="gene ID" value="ENSCING00000002295.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000169452"/>
    <dbReference type="HOGENOM" id="CLU_051922_2_0_1"/>
    <dbReference type="InParanoid" id="F6URF6"/>
    <dbReference type="OMA" id="QMAIYAD"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14695">
        <property type="entry name" value="bZIP_HLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000007">
        <property type="entry name" value="hepatic leukemia factor isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040223">
        <property type="entry name" value="PAR_bZIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11988:SF27">
        <property type="entry name" value="GH27708P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11988">
        <property type="entry name" value="THYROTROPH EMBRYONIC FACTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07716">
        <property type="entry name" value="bZIP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="9">
        <location>
            <begin position="324"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="92"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="200"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="356"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="99"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="223"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000004693.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="393" mass="44546" checksum="ACAD701A533EFA85" modified="2012-04-18" version="2">MELSASANDEEELSLTLQFLLENPALLGHQDVNNNDEKCEEKCGEASEQTDLASAYLGPQIWNDMLLSDDLKLEPVDLDDLLKENDVNGETSEFQFQMSPASSCVSTTSSSAEPQDSSQLYQDMPGLVAEQTFSAATYNQQWIHNSFKSSQTPLQNLPSKVDYTQNVYARSPDHYPNYKMESNKLREQQMAIYADIMAASPHGINIPPSPTMEKQQQQQQQQPSTSHATEASDTTSNDNHKPPTYKYNPAPLISIPPIEIRLPTPVKVDFTPSSADVLISTPQSESGEINEFNPCTRQFTDDELKPKPMVRKSRKVHVSSDSKDVKYWNRRNKNNVAAKRSREARRIKENQIAMRANFLEKENESLKMEVADLRSELKRVMNTLRVYEKEIIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>F6UVC2</accession>
    <name>F6UVC2_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phosphatidylinositol-3,4-bisphosphate 4-phosphatase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01001285">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6UVC2"/>
    <dbReference type="FunCoup" id="F6UVC2">
        <property type="interactions" value="18"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000014888"/>
    <dbReference type="Ensembl" id="ENSCINT00000014888.3">
        <property type="protein sequence ID" value="ENSCINP00000014888.3"/>
        <property type="gene ID" value="ENSCING00000007257.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000168191"/>
    <dbReference type="HOGENOM" id="CLU_007802_0_0_1"/>
    <dbReference type="InParanoid" id="F6UVC2"/>
    <dbReference type="OMA" id="MEHTINI"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016316">
        <property type="term" value="F:phosphatidylinositol-3,4-bisphosphate 4-phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039034">
        <property type="entry name" value="INPP4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12187">
        <property type="entry name" value="AGAP000124-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12187:SF11">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-3,4-BISPHOSPHATE 4-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000014888.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="898" mass="101163" checksum="476ECD4B6A6E1DC3" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>F6UVD1</accession>
    <accession>A0A1W2WAK9</accession>
    <name>F6UVD1_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">J domain-containing protein-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100176568</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
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                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
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                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000002888.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="251" mass="26100" checksum="F3E7D89CDFAC399A" modified="2012-04-18" version="2">MKVFILALLVAAAGNYDVSIADDDKIIGGQLTSPGDLPYIVNLKRFGSLMCGGSILSANWAMSAAHCNAAAASLTVTAGDYSLSNNDGTEQASSVVRTVPHPQYNSNTMENDIMLLQLSSSISQNSHVTFATLPAAFNDPAVGTVCKVSGWGNTQIVGSNYPDKPYSVQVPIISNSDCNNAYGGGIYDGMMCLGLLGTGGKDSCQGDSGGPVVCNGAVHGVVSWGIGCAYPDYPGVYTRVSHYISFINSYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>F6UYT5</accession>
    <name>F6UYT5_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Adenylate cyclase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">wild type</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01001190">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6UYT5"/>
    <dbReference type="STRING" id="7719.ENSCINP00000026290"/>
    <dbReference type="Ensembl" id="ENSCINT00000026536.2">
        <property type="protein sequence ID" value="ENSCINP00000026290.2"/>
        <property type="gene ID" value="ENSCING00000020025.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158054"/>
    <dbReference type="HOGENOM" id="CLU_887194_0_0_1"/>
    <dbReference type="InParanoid" id="F6UYT5"/>
    <dbReference type="OMA" id="NENWHAL"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032628">
        <property type="entry name" value="AC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45627:SF16">
        <property type="entry name" value="ADENYLATE CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45627">
        <property type="entry name" value="ADENYLATE CYCLASE TYPE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16214">
        <property type="entry name" value="AC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="101"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="130"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="159"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="Adenylate cyclase N-terminal" evidence="5">
        <location>
            <begin position="35"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF16214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000026290.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="314" mass="36407" checksum="E7EFC387B9729683" modified="2012-04-18" version="2">MLPMQSFSPIQQPEPTPEVELNSRSPKMLTKCCFWSSCAEMLHKFMSGVRFESWSSERLYQRYFFHSKINMLSILLWLLKSLCIILFCFKYCTGQNWNDMVYGIIIIVLLLLCIALQVIFLLNVLSQHTLFLVSYIILAMVVIIDLVSVVLLERSSSSYMVWLTLFMVYIMYTMIPLNILVVSAASFILSCSHMLVSYVSNGPKPHYPNQIASDFLLFFCTNVVGMCIRITSELAQRKAFTETRECIVARLNLQRQNLHQERLLLSVLPRHVAMEMKGDIDQSKPKTQQFYKIYIQKHNNVSILYADIEGFTKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F6V1P3</accession>
    <name>F6V1P3_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized LOC100182670</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100182670</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="F6V1P3"/>
    <dbReference type="Ensembl" id="ENSCINT00000012947.3">
        <property type="protein sequence ID" value="ENSCINP00000012947.3"/>
        <property type="gene ID" value="ENSCING00000006279.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000066534"/>
    <dbReference type="HOGENOM" id="CLU_674322_0_0_1"/>
    <dbReference type="InParanoid" id="F6V1P3"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003343539" evidence="3">
        <location>
            <begin position="24"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="261"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="380"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="385"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000012947.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="408" mass="46256" checksum="13FF6046C52AB2A5" modified="2012-04-18" version="2">MFEVIFNCLLFFVILTGCDVTKGGRNVTLDCASQRPVSCERKIFVRQNRGVLVSPTKSRLDHSCCWSGNWVDVTSQRLGETSVGNETLMTCFNMDERKVEKVSMKFMAQVIEGREVAWFKAKLKPSADYKVVSCKQHLYNGSTHEDRTTLLVKFPPLFLRHVIKFNVMIGRMNAIDIPVFSHPANMRRRNVRIQKQGLNFTHFYLRHTGRNRQDIVMHFPQLSINDVGNYSVVIHTSHHVVSTVVILNPSNKDEGVNENHVIIIVISCLCVVLVVMVVLCCFYGSKPRCRIRSSSSNGASLVGLNIDVAESTGVGDDVFLPVPPRSKKQSLKSLKRETIDQSKFDSEVTYMNDDVGNDDVDEDGYLQPIRINEHDYVAFDDGQRHQATTPPAPSRTNQDETQFTYENF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>F6V3W8</accession>
    <name>F6V3W8_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BCAS3 WD40 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <dbReference type="AlphaFoldDB" id="F6V3W8"/>
    <dbReference type="STRING" id="7719.ENSCINP00000024644"/>
    <dbReference type="Ensembl" id="ENSCINT00000024890.2">
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        <location>
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            <begin position="1"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    <sequence length="264" mass="28546" checksum="5C11F42C9D37E51B" modified="2011-07-27" version="1">MSGTTFSGENKVQQSNRRRMDSSGGRCVKPEPANGKSYMESVYNFIHDLSQAYTKSAIPDEKDTITWARFCSYPDAPEVNESSINPPMLLLLGYSNGIQMWNLQINGDAQEVFSLRDGPVKTACVLPPPPASDIIDQLQEKRPLIAICLAANSKQMFAQVTIRSLRSGDQVKRLDFPSDVISLHAGPAPLLLVALKESVHGFDARTLTQVFILNGCHPSGGFIANPISLGRRWLAYSPSIPLPGLQSSGGVAGDGVPSYVATVI</sequence>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_002128820.3"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="2"/>
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            <dbReference type="Ensembl" id="ENSCINP00000024901.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
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                <person name="Inaba K."/>
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                <person name="Kobayashi K."/>
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                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
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    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome 8"/>
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    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
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    </dbReference>
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        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR20765">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 43 MEMBER 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="74"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="128"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="153"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="192"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="284"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="324"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="369"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="404"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="429"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="459"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="245"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000024313.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="495" mass="54524" checksum="83C0530BE9857565" modified="2012-04-18" version="2">FCHNMKHKQVLSVIAALFDTLLFGGIIFGWPSLDFVLKREGYFSSKCEIETGDLKNGTTVASSEKSLNCPSQDIALNLVYTLSTTIGGVSTFPLGYLFDRFGAWKFRTGSILTFILACTAIACSNKETSWLLYIAMPVVAVCSFAILTSNTPIGYFCVAAQATVISIIYGAFDSSAAVMVVMKIASQLGATVAEIFTFYGALLLLSLVYTFVLMPRMHVPFNVPDGYQYGVTECCCFNNPISSPQNTNTPNQSRRKRSGVSESTPLLANQGEEQKTFSSTLKDLSYWLNVLYLSILQLRLYVFYGTLNQWISTLTSNREQQSKYITAFGFIQLTGILIAPFSGVMTDMMTKYYRKSVTCSEQVVRKRGVCFTIGCTCLMATIFSALVLIPVLEIQYITFFVQNAARTFLYAGNGAFLATTFPSEHFGKLNGLTIGIASVVSLLTYPINILVLKVFGGSFVVVDSVFLGLCVLTFVHPVVLFFRTKPSKKTSTCYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>F6VYY1</accession>
    <name>F6VYY1_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">wild type</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="EAAA01002454">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6VYY1"/>
    <dbReference type="FunCoup" id="F6VYY1">
        <property type="interactions" value="5"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000022514"/>
    <dbReference type="Ensembl" id="ENSCINT00000022760.2">
        <property type="protein sequence ID" value="ENSCINP00000022514.2"/>
        <property type="gene ID" value="ENSCING00000011895.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157161"/>
    <dbReference type="HOGENOM" id="CLU_000288_63_23_1"/>
    <dbReference type="InParanoid" id="F6VYY1"/>
    <dbReference type="OMA" id="SECINAG"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000006">
        <property type="entry name" value="Serine/threonine-protein kinase WNK1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050588">
        <property type="entry name" value="WNK_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13902">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE WNK WITH NO LYSINE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000654">
        <property type="entry name" value="Integrin-linked_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="1"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000022514.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="220" mass="25312" checksum="687A4ACBAB7AB7A8" modified="2012-04-18" version="2">MLKGLQHPNIVRFYDSWDYQSLKGKKCIILVTELMTSGTLKTYLKRFKSIKPKVLRSWCRQILKGLNFLHTRNPAIIHRDLKCDNIFITGPTGSVKVGDLGLATLKRTSFAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMITAEYPYSECINAGQIYRKVTQGLPPNSFEKVQGKDERRIISICINRDKTQRYTVQQLLNEPFFVEVPGIKVEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>F6W5C2</accession>
    <name>F6W5C2_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Abnormal spindle-like microcephaly-associated protein ASH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
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                <person name="Doyle S."/>
                <person name="Francino P."/>
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                <person name="Haga S."/>
                <person name="Hayashi H."/>
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                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
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                <person name="Zhang C."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
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                <person name="Branno M."/>
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                <person name="Shoguchi E."/>
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                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
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                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
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                <person name="Richardson P."/>
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                <person name="Kohara Y."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="21" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="21">wild type</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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        <scope>IDENTIFICATION</scope>
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    <keyword evidence="14 18" id="KW-0464">Manganese</keyword>
    <keyword evidence="16 18" id="KW-0469">Meiosis</keyword>
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        <location>
            <begin position="296"/>
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            <position position="129"/>
        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
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        <source>
            <dbReference type="PIRSR" id="PIRSR000882-1"/>
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        <source>
            <dbReference type="RuleBase" id="RU003447"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="SMART" id="SM01347"/>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000010283.3"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    </evidence>
    <sequence length="530" mass="60318" checksum="E3D619FC29C4D05C" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>F6WRM0</accession>
    <name>F6WRM0_CIOIN</name>
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        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
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    <reference evidence="7" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">wild type</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytochrome P450 family.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="ENSCING00000002119.3"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
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        <property type="project" value="InterPro"/>
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        <location>
            <begin position="6"/>
            <end position="21"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000004337.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="247" mass="28223" checksum="9E7066BED09D2C80" modified="2012-04-18" version="2">MLPSGYIFVFVFLCIYYLLQWRRRPKNFPPGPLGIPLFGIAPFAGVDMHKYLATYYAKYGGVMSFRLATKDWIVLNDIEAITQALLKQGESFSGRPQSYLMNQLTEGCGIVFSTGPRWQAQRRFVLTALKTLGMGKRSMVGIINEENQNFLSVIQSSGGKVNILSEFRKLLSNIVTNLIMGKRFNYEDEKLQAIVDHRPTSSVVMFIPFLRFIPPFKQGYQRLIASTQRVLDIISEITEEHKNSFDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F6XA77</accession>
    <name>F6XA77_CIOIN</name>
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        <recommendedName>
            <fullName evidence="3">CUB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
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                <consortium name="Ensembl"/>
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        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
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                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
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                <person name="Ho I."/>
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                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
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                <person name="Inaba K."/>
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                <person name="Kobayashi K."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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</entry>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Campbell R.K."/>
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                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
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                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Putnam N."/>
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                <person name="Terry A."/>
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                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">wild type</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01001793">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6XG67"/>
    <dbReference type="STRING" id="7719.ENSCINP00000019976"/>
    <dbReference type="Ensembl" id="ENSCINT00000019976.3">
        <property type="protein sequence ID" value="ENSCINP00000019976.3"/>
        <property type="gene ID" value="ENSCING00000009856.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155587"/>
    <dbReference type="HOGENOM" id="CLU_1685946_0_0_1"/>
    <dbReference type="InParanoid" id="F6XG67"/>
    <dbReference type="OMA" id="LMEFFNQ"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004114">
        <property type="term" value="F:3',5'-cyclic-nucleotide phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1300.10">
        <property type="entry name" value="3'5'-cyclic nucleotide phosphodiesterase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PDEase_catalytic_dom"/>
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    <dbReference type="InterPro" id="IPR036971">
        <property type="entry name" value="PDEase_catalytic_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11347">
        <property type="entry name" value="CYCLIC NUCLEOTIDE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00233">
        <property type="entry name" value="PDEase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51845">
        <property type="entry name" value="PDEASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDEase" evidence="4">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="105"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="108"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000019976.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="156" mass="17703" checksum="AFDE2A5907EF20F8" modified="2012-04-18" version="2">MMALQKILIKCCDISNEVRPLTCSEQWVDLLFEEFFVQADRERREGLPVASFMNRSQVHKADDQLKFIRGIILPLFRCLGKVFPDVNTKILKSLQESIRYYENMTTASKHENDGTDVSPTDKKETQKEAHKESKAAAKTTVSAVPKIVVSLPNGTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>F6XN50</accession>
    <name>F6XN50_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">wild type</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="EAAA01001944">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6XN50"/>
    <dbReference type="STRING" id="7719.ENSCINP00000024894"/>
    <dbReference type="Ensembl" id="ENSCINT00000025140.2">
        <property type="protein sequence ID" value="ENSCINP00000024894.2"/>
        <property type="gene ID" value="ENSCING00000013587.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153981"/>
    <dbReference type="HOGENOM" id="CLU_021549_3_0_1"/>
    <dbReference type="InParanoid" id="F6XN50"/>
    <dbReference type="OMA" id="YTWSGCA"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019236">
        <property type="term" value="P:response to pheromone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053880">
        <property type="entry name" value="GPR180-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047831">
        <property type="entry name" value="GPR180/TMEM145"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019336">
        <property type="entry name" value="GPR180/TMEM145_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23252">
        <property type="entry name" value="INTIMAL THICKNESS RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23252:SF24">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 145"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10192">
        <property type="entry name" value="GPR180-TMEM145_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21892">
        <property type="entry name" value="TMEM145_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003345407" evidence="7">
        <location>
            <begin position="19"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="184"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="216"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="250"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="291"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="323"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="356"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="domain" description="GPR180-like N-terminal" evidence="9">
        <location>
            <begin position="22"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="GPR180/TMEM145 transmembrane" evidence="8">
        <location>
            <begin position="186"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF10192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF21892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000024894.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="450" mass="51316" checksum="4A68EDD8E415F60F" modified="2012-04-18" version="2">MYLLTLLVGVLTVTCCSGLYLEGRLFTREDWSFLSRFCFLSDIGRMQFKFVFPYADCCNNILLYFDDGVQWPKVYKNSSKTCHQKEAVLEPENNQIINLTTRYTWSGCVLHNSSGENSGVLVCEGGRSFRSKHERWWYIAISNCKSGQLGKGIDLTYQVKLTNGHSFWTEHFSADEFGILETNLFFLIVFALMLILSIHVAVSLQNRQMLHTTYKMFMVCIALEVFGLLMLCVAIGTYASDGIGNNGLKLIGEILGTSAELLFLLLLLLLGKGFTITRGHLSYAGTIKMSVFITVYAVIDVILFLYEYILFDPGLVLYVYESPAGYGLIGLRLAAWVWFSYGIIFTLKHYPEKNKFYYPLYGIYTIWFYAIPIIVLVATFAIPRYMRRKVVNGLQRCIDFLAYSVFLTLMRPQAANRNFPYHVRTSQIGTVVSVTDGKTHATSNFPHNMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>F6XQX8</accession>
    <name>F6XQX8_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">cyclin-dependent kinase</fullName>
            <ecNumber evidence="3">2.7.11.22</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">wild type</strain>
        </source>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.22" evidence="3"/>
    <dbReference type="EMBL" id="EAAA01002255">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6XQX8"/>
    <dbReference type="FunCoup" id="F6XQX8">
        <property type="interactions" value="266"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000005172"/>
    <dbReference type="Ensembl" id="ENSCINT00000005172.3">
        <property type="protein sequence ID" value="ENSCINP00000005172.3"/>
        <property type="gene ID" value="ENSCING00000002540.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000167918"/>
    <dbReference type="HOGENOM" id="CLU_000288_181_1_1"/>
    <dbReference type="InParanoid" id="F6XQX8"/>
    <dbReference type="OMA" id="YEEHRVI"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030332">
        <property type="term" value="F:cyclin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010389">
        <property type="term" value="P:regulation of G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07838">
        <property type="entry name" value="STKc_CDK4_6_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000124">
        <property type="entry name" value="Cyclin-dependent kinase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000205">
        <property type="entry name" value="Cyclin-dependent kinase 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF472">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 4, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 15 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="9 15 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 16" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="18">
        <location>
            <begin position="36"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="16"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000005172.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="324" mass="36502" checksum="947D1C351F5D7BE0" modified="2012-04-18" version="2">MTSEQSHDADEAPSARASQSSLHAEPTSSPWTNQRYHTLSEIGVGAYGVVFKAQDMQSDDENKFVAIKCVRVENSEQGMPLSTVREIALLRQLESCEHPNVVRLLDICAGHHSKRETQLMLVFEYVDQDLDVFLKKCPDSGLEPEKVTDIMRQMLGGLDFLHSMRVVHRDMKPQNILITTTGQVKIADFGLARIYSIDMALTEVVVTLWYRSPEVLLLDSYATPVDIWSAGCIFAELFNRRPLFRGTGDVNQLKKIFDFIGRPSEAEWPLNVAVPIDSFPPQPPRPPQKFVPSITEDAADFLMKLLVFDPGKRLTAQSALQHKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F6XR85</accession>
    <name>F6XR85_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
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                <person name="Haga S."/>
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                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
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                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
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                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
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                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
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                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="9" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="9">wild type</strain>
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    <reference evidence="9" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
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        <text evidence="1 6">Belongs to the universal ribosomal protein uL15 family.</text>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Suzuki M.M."/>
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                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">wild type</strain>
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    <reference evidence="1" key="3">
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<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
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        <name type="scientific">Ciona intestinalis</name>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DNA mismatch repair MutS family.</text>
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    <dbReference type="STRING" id="7719.ENSCINP00000003059"/>
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        <property type="protein sequence ID" value="ENSCINP00000003059.3"/>
        <property type="gene ID" value="ENSCING00000001547.3"/>
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    <dbReference type="GeneTree" id="ENSGT00550000074977"/>
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    <dbReference type="InParanoid" id="F6YKR1"/>
    <dbReference type="OMA" id="CSVYFMP"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="DNA mismatch repair protein MSH5"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
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        <property type="entry name" value="DNA_mismatch_repair_Msh2"/>
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        <property type="entry name" value="DNA_mismatch_repair_MutS_core"/>
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        <property type="entry name" value="DNA_mismatch_repair_MutS_sf"/>
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    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
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        <property type="entry name" value="MUTS PROTEIN HOMOLOG 5"/>
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        <property type="entry name" value="MutS_III"/>
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    <dbReference type="Pfam" id="PF00488">
        <property type="entry name" value="MutS_V"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="MSH2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="MUTSac"/>
        <property type="match status" value="1"/>
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        <location>
            <begin position="619"/>
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        <location>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006271"/>
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    </evidence>
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        <source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
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        <source>
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            <dbReference type="ARBA" id="ARBA00023254"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00073549"/>
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        <source>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000003059.3"/>
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        <source>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>F6YY07</accession>
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            <fullName evidence="8">Mediator of RNA polymerase II transcription subunit 27</fullName>
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        <name type="scientific">Ciona intestinalis</name>
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        <name type="synonym">Ascidia intestinalis</name>
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            <taxon>Phlebobranchia</taxon>
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            <taxon>Ciona</taxon>
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    <reference evidence="7" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">wild type</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Mediator complex subunit 27 family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002729">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="interactions" value="328"/>
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    <dbReference type="STRING" id="7719.ENSCINP00000024252"/>
    <dbReference type="Ensembl" id="ENSCINT00000024498.2">
        <property type="protein sequence ID" value="ENSCINP00000024252.2"/>
        <property type="gene ID" value="ENSCING00000013154.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000012207"/>
    <dbReference type="HOGENOM" id="CLU_056015_0_0_1"/>
    <dbReference type="InParanoid" id="F6YY07"/>
    <dbReference type="OMA" id="FHEDCRN"/>
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        <property type="component" value="Chromosome 8"/>
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    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0000318"/>
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        <property type="term" value="F:transcription coactivator activity"/>
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        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
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        <property type="entry name" value="Mediator_Med27"/>
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        <property type="entry name" value="34 KDA TRANSCRIPTIONAL CO-ACTIVATOR-RELATED"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 27"/>
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    </dbReference>
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        <property type="entry name" value="Med27"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000024252.2"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="318" mass="36414" checksum="14493A39CEFBE560" modified="2012-04-18" version="2">MADTINQAKLTISCIAQAITSTQQLRSTVTNVFNLLCEGVKPDEDHDGRGRDRKKFLQKFQEHLQAVNKDYNEVEKYCNSMIPTPELQVLGPLSHLSLDSNTDKKNTFRDAVQTYEWNQRMHEYAQIASSILLSHQLKRSQSSGGAILAKRRRALPPKFQNMSSQHVENTLQLLARQPADLTFKLIKTLGDTRILQVTVGRVFTVILMLRNLLMEKVLVKGLDEELYYENGTIDLFSESQFECMRRITDHAMTAVLQYSTTHATQPEIPLKYFLLWLQSYKNLFIDPCSICGKHLDGGLPPVWRDFRNPVACHDTCRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>F6Z010</accession>
    <accession>A0A1W2W9D6</accession>
    <name>F6Z010_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Phospholipase ABHD3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100175495</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">wild type</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily. AB hydrolase 4 family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01000619">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002127021.1">
        <property type="nucleotide sequence ID" value="XM_002126985.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6Z010"/>
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        <property type="interactions" value="14"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000015883"/>
    <dbReference type="Ensembl" id="ENSCINT00000015883.3">
        <property type="protein sequence ID" value="ENSCINP00000015883.3"/>
        <property type="gene ID" value="ENSCING00000007743.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100175495"/>
    <dbReference type="KEGG" id="cin:100175495"/>
    <dbReference type="GeneTree" id="ENSGT00950000182902"/>
    <dbReference type="HOGENOM" id="CLU_032487_4_0_1"/>
    <dbReference type="InParanoid" id="F6Z010"/>
    <dbReference type="OMA" id="LDWHGPH"/>
    <dbReference type="OrthoDB" id="247542at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008126">
        <property type="term" value="F:acetylesterase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047372">
        <property type="term" value="F:monoacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051792">
        <property type="term" value="P:medium-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051793">
        <property type="term" value="P:medium-chain fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000483">
        <property type="entry name" value="Clan SC, family S33, methylesterase-like serine peptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AB_hydrolase_4_sf"/>
    </dbReference>
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        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012020">
        <property type="entry name" value="ABHD4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10794">
        <property type="entry name" value="ABHYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10794:SF63">
        <property type="entry name" value="ALPHA_BETA HYDROLASE 1, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ab_hydro_YheT"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine aminopeptidase S33" evidence="3">
        <location>
            <begin position="160"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="2">
        <location>
            <position position="188"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="2">
        <location>
            <position position="315"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="2">
        <location>
            <position position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR005211-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000015883.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="378" mass="42451" checksum="94CACCB9015C11AE" modified="2011-07-27" version="1">MIELSPATALLTGTVCYWAYYLTHYGKKPELHHKKGTKFDSFLENHVPKLKIPYWSFFWVPGGKLQTIVASLLPVKYNVEYTREAVMTSDGGQFTLAWANKEEGNQHSPVVVILPGLVSTSETGYIKCIVDAVLKKGHRAVVVVNRGLELPCLTGKAFCATHTSDFEQAMEIIKQRNPNIPVMAIGVSLGSLILGRYLSIKQEKSQLQAAFLYSCPFDTVRGSASIEKWDNWLLYNIVLTNNLKQIYIRHKEKFEDIVDHTEVMGSRSIREVDMHFTSKVFGYESVDHYYQDSRLTAKRLQSIQIPTLCLCANDDAFIPDSALPRNDVKETEYVALAVTSGGGHVSHLSGANPFTTPYYIDVISDYMTAVFEHGTDLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F6ZFH4</accession>
    <name>F6ZFH4_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">GDNF/GAS1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">wild type</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="EAAA01001480">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6ZFH4"/>
    <dbReference type="Ensembl" id="ENSCINT00000017684.3">
        <property type="protein sequence ID" value="ENSCINP00000017684.3"/>
        <property type="gene ID" value="ENSCING00000008665.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000001613"/>
    <dbReference type="HOGENOM" id="CLU_1264076_0_0_1"/>
    <dbReference type="InParanoid" id="F6ZFH4"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016017">
        <property type="entry name" value="GDNF/GAS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037193">
        <property type="entry name" value="GDNF_alpha"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02351">
        <property type="entry name" value="GDNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00907">
        <property type="entry name" value="GDNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110035">
        <property type="entry name" value="GDNF receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003347688" description="GDNF/GAS1 domain-containing protein" evidence="6">
        <location>
            <begin position="23"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="GDNF/GAS1" evidence="7">
        <location>
            <begin position="34"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000017684.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="219" mass="24089" checksum="C57CEF9EF1CFC5E3" modified="2012-04-18" version="2">MEYSQFLLLFVVLTAIFCQVQSVGFDVGDTRPSCLQSLKNCKRNSTCKEVYKSVKNKCKAKEAQCNARTADLQQCAMDIAYLREAAFPIDNKCICHGKSLSLKKLVRCSEILNTVYANPCFEKGERIRQEAIRIASVTMRAAVATEIDDVTQTPKYDVVSDVALMASTTSAPDTLGENLGEVAADQGDIKSGVRVSDVEKKMESVSEEDMQDVDALFAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F6ZK90</accession>
    <name>F6ZK90_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="F6ZK90"/>
    <dbReference type="Ensembl" id="ENSCINT00000027795.2">
        <property type="protein sequence ID" value="ENSCINP00000027549.2"/>
        <property type="gene ID" value="ENSCING00000015612.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00660000096876"/>
    <dbReference type="HOGENOM" id="CLU_1424447_0_0_1"/>
    <dbReference type="InParanoid" id="F6ZK90"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000027549.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="191" mass="20384" checksum="A903318F09FED9C7" modified="2012-04-18" version="2">MQQDGHDVPLDTPYTVVDFDENGGTIERNVHLVHGETTQFVEDVQQVLCSSANINSLIDRDVSAITNLPSMQSRDSTPVITPSGYCQPSAVTDTVQSTNAASLPILENILNPNSAVNEPQQMNDSSDDLLKMMSAMSISSGSQPTVLHLNKSEDKVSDGPLLSGSLISEDGSISSVNDSPSTVVADDINFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>F6ZKL4</accession>
    <name>F6ZKL4_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S1 family. CLIP subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="F6ZKL4"/>
    <dbReference type="STRING" id="7719.ENSCINP00000027536"/>
    <dbReference type="Ensembl" id="ENSCINT00000027782.2">
        <property type="protein sequence ID" value="ENSCINP00000027536.2"/>
        <property type="gene ID" value="ENSCING00000015598.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162823"/>
    <dbReference type="HOGENOM" id="CLU_006842_13_1_1"/>
    <dbReference type="InParanoid" id="F6ZKL4"/>
    <dbReference type="OMA" id="IRTTIEN"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000002">
        <property type="entry name" value="Transmembrane protease serine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051487">
        <property type="entry name" value="Ser/Thr_Proteases_Immune/Dev"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24256">
        <property type="entry name" value="TRYPTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase S1" evidence="3">
        <location>
            <begin position="1"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000027536.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="190" mass="20902" checksum="FBB54EF20EDC79FF" modified="2011-07-27" version="1">MHRLSEDGERYFIRRAIVHPNYIYRDVTDADIALVELRSEVPLTTEVVPVCLPQFSQPLVNETCFITGWGFTGPASSVSARLSPTLLEAEVPIMNFTACQMLSPIYQELLNSTNHMCAGDPVTAATDTCLGDSGGPLTCQRLDNTWYISGVTSFSFVECGTPGHVGIYTPLISFEEWIRTTIENNTNAAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>F6ZKS0</accession>
    <name>F6ZKS0_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Cytoplasmic dynein 2 heavy chain 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Cytoplasmic dynein 2 heavy chain</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Dynein cytoplasmic heavy chain 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">Dynein heavy chain isotype 1B</fullName>
        </alternativeName>
    </protein>
    <organism evidence="25 26">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">wild type</strain>
        </source>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="19">The cytoplasmic dynein complex 2 is probably composed by a heavy chain DYNC2H1 homodimer and a number of DYNC2LI1 light intermediate chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the dynein heavy chain family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001179">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="F6ZKS0">
        <property type="interactions" value="14"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000003581"/>
    <dbReference type="Ensembl" id="ENSCINT00000003581.3">
        <property type="protein sequence ID" value="ENSCINP00000003581.3"/>
        <property type="gene ID" value="ENSCING00000001762.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154620"/>
    <dbReference type="HOGENOM" id="CLU_000038_3_0_1"/>
    <dbReference type="InParanoid" id="F6ZKS0"/>
    <dbReference type="OMA" id="WCKERVS"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097729">
        <property type="term" value="C:9+2 motile cilium"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005868">
        <property type="term" value="C:cytoplasmic dynein complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008569">
        <property type="term" value="F:minus-end-directed microtubule motor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0060294">
        <property type="term" value="P:cilium movement involved in cell motility"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035721">
        <property type="term" value="P:intraciliary retrograde transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Cytoplasmic dynein 1 heavy chain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="cytoplasmic dynein 2 heavy chain 1"/>
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        <property type="entry name" value="Cytoplasmic dynein 2 heavy chain 1"/>
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    <dbReference type="Gene3D" id="1.20.140.100">
        <property type="entry name" value="Dynein heavy chain, N-terminal domain 2"/>
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        <property type="entry name" value="Dynein heavy chain, N-terminal domain 2"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="5"/>
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        <property type="entry name" value="Region D6 of dynein motor"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="AAA+_ATPase"/>
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        <property type="entry name" value="AAA_6"/>
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        <property type="entry name" value="AAA_9"/>
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        <property type="entry name" value="AAA_lid_11"/>
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        <property type="entry name" value="AAA_lid_11_sf"/>
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        <property type="entry name" value="DHC"/>
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        <property type="entry name" value="DYNC2H1-like_lid"/>
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        <property type="entry name" value="DYNC2H1_AAA_dom"/>
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    <dbReference type="InterPro" id="IPR042222">
        <property type="entry name" value="Dynein_2_N"/>
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    <dbReference type="InterPro" id="IPR043157">
        <property type="entry name" value="Dynein_AAA1S"/>
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        <property type="entry name" value="Dynein_C"/>
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        <property type="entry name" value="Dynein_C_barrel"/>
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        <property type="entry name" value="Dynein_HC_stalk"/>
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        <property type="entry name" value="Dynein_heavy_chain_D4_dom"/>
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        <property type="entry name" value="Dynein_heavy_D6_P-loop"/>
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        <property type="entry name" value="Dynein_heavy_linker"/>
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        <property type="entry name" value="Dynein_linker_3"/>
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        <property type="entry name" value="P-loop_NTPase"/>
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        <property type="entry name" value="DYNEIN CYTOPLASMIC 2 HEAVY CHAIN 1"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR45703">
        <property type="entry name" value="DYNEIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="AAA_6"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="AAA_7"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="AAA_8"/>
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        <property type="entry name" value="AAA_9"/>
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        <property type="entry name" value="Dynein_C"/>
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        <property type="entry name" value="Dynein_heavy"/>
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        <property type="entry name" value="MT"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="AAA"/>
        <property type="match status" value="3"/>
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    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="4"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="17" id="KW-0966">Cell projection</keyword>
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    <keyword evidence="9" id="KW-0970">Cilium biogenesis/degradation</keyword>
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    <keyword evidence="16" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="11" id="KW-0243">Dynein</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0493">Microtubule</keyword>
    <keyword evidence="15" id="KW-0505">Motor protein</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="24">
        <location>
            <begin position="789"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="24">
        <location>
            <begin position="1081"/>
            <end position="1236"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="24">
        <location>
            <begin position="1394"/>
            <end position="1546"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="23">
        <location>
            <begin position="2204"/>
            <end position="2280"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="23">
        <location>
            <begin position="2527"/>
            <end position="2579"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00064100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00078768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00083259"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00083782"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000003581.3"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>F6ZRC0</accession>
    <accession>A0A1W2WQF7</accession>
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        <submittedName>
            <fullName evidence="2">F-box only protein 36-like</fullName>
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        <name evidence="2" type="primary">LOC100186925</name>
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    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
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            <taxon>Phlebobranchia</taxon>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
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    <dbReference type="Ensembl" id="ENSCINT00000016254.3">
        <property type="protein sequence ID" value="ENSCINP00000016254.3"/>
        <property type="gene ID" value="ENSCING00000007945.3"/>
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        <property type="component" value="Chromosome 1"/>
    </dbReference>
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        <property type="entry name" value="F-box-like_dom_sf"/>
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    </dbReference>
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    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="104"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000016254.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="192" mass="22521" checksum="AAF597D94DF1B9FF" modified="2011-07-27" version="1">MKSLFKNGELLNVSEQAPSPSKDFINLIVTMKEVIWRYWKINPQCLKSDVSIAPSENRQSHDDFIHDLDLHSEVKRVFGVNMLVYIKQLCLGHFDYFVRLSNPLQILIMSYMELEDIASLSQTNKHFHELCNSEQLWEHIVESHCDTITPEMKLFAGEVGWKHVFFTNKLQLRAQMRRHQNRGTFSNAPDEQ</sequence>
</entry>
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        <lineage>
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            <taxon>Ciona</taxon>
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    <reference evidence="17" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
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            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">wild type</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. Lammer subfamily.</text>
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        <property type="entry name" value="Kinase-like_dom_sf"/>
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        <property type="entry name" value="Ser/Thr_kinase_AS"/>
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        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE DOA-RELATED"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR45646:SF11">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE DOA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 12 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 13" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="149"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="473"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="27"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="53"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="82"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="97"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="477"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000016222.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="497" mass="59182" checksum="0DC37890885CE7AA" modified="2012-04-18" version="2">MGKTSRRYRSRENDENYKYGQKRRRVEKYDDYYEDTYGPKRCNDSYSCEKHSYSSQSGKHYQSSYNDGYYSRDSSRTLNSRQKNEDSRDRHDYSGSSRRRRSRHRKHKSRSSHSRDRHRNKNGGRAKSVEDDEEGHLIYRHGDCLQARYEILSTLGEGTFGKVLLCLDHQRGGKKVALKVIKNIEKYRDAAFLEINVLKKLQEKDPVGKYLCVTMLDWFNYHGHICLAFELLGLSTYDFQKDNHYIPYPLDQVRHMAFQLVWAVKFLHDNHITHTDLKPENILFRDSLCELRYNPSKRRDERQLVSSDIRLIDFGSATFDNEHHSTVVSTRHYRAPEVVLELGWNQSCDVWSIGCIIFEFYMGITMFQTHDNREHLAMMERILGPIPSRMIKKSRKQKYFYHNRLDWDEHSSGGKYVRENCKPIMRYIQRKDHPEHMKLIDLIARMLEYDPTKRISLAEAINHSFFDELPHEKRFPNNHTNQSSYETNTRCLRSAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>F7AFM4</accession>
    <name>F7AFM4_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Carbohydrate sulfotransferase</fullName>
            <ecNumber evidence="9">2.8.2.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">wild type</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Golgi apparatus membrane</location>
            <topology evidence="1 9">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the sulfotransferase 2 family.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="9"/>
    <dbReference type="EMBL" id="EAAA01001304">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F7AFM4"/>
    <dbReference type="STRING" id="7719.ENSCINP00000026038"/>
    <dbReference type="Ensembl" id="ENSCINT00000026284.2">
        <property type="protein sequence ID" value="ENSCINP00000026038.2"/>
        <property type="gene ID" value="ENSCING00000014384.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164429"/>
    <dbReference type="HOGENOM" id="CLU_106532_0_0_1"/>
    <dbReference type="InParanoid" id="F7AFM4"/>
    <dbReference type="OMA" id="LMYCEIP"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016051">
        <property type="term" value="P:carbohydrate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018011">
        <property type="entry name" value="Carb_sulfotrans_8-10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005331">
        <property type="entry name" value="Sulfotransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12137">
        <property type="entry name" value="CARBOHYDRATE SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12137:SF54">
        <property type="entry name" value="CARBOHYDRATE SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03567">
        <property type="entry name" value="Sulfotransfer_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="8 9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000026038.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="227" mass="26105" checksum="FA1B1497B6FCCD28" modified="2012-04-18" version="2">MVSKNTRELEKVEVTREAGYLRKPLSPEVSDVEDATPDVAPVKEDVLESEDEFMKRIEKRMRKRREHLQQACERLGLQPNKTDGHILDHLPRPMPWSFVSNKHKLMYCEIPKSGCTNWKKVLMVLNGVLNNTDNIKQGEAHTKSLQMLRMMPNAERKRFYEAATKLLVVRQPFERLVSAYADKVAAKPPANDSVFFQFSSEVAKKYGRRQIDSDTTILNDGTRSTTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>F7APA8</accession>
    <name>F7APA8_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Exostosin-like 3</fullName>
            <ecNumber evidence="22">2.4.1.223</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="25">Glucuronyl-galactosyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="24">Multiple exostosis-like protein 3</fullName>
        </alternativeName>
    </protein>
    <organism evidence="30 31">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="31" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="30" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="30">wild type</strain>
        </source>
    </reference>
    <reference evidence="30" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="30" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="20">Glycosyltransferase which regulates the biosynthesis of heparan sulfate (HS). Initiates HS synthesis by transferring the first N-acetyl-alpha-D-glucosamine (alpha-GlcNAc) residue (GlcNAcT-I activity) to the tetrasaccharide linker (GlcA-Gal-Gal-Xyl-)Ser core linker. May also transfer alpha-GlcNAc residues during HS elongation (GlcNAcT-II activity). Lacks glucuronyl transferase II (GlcAT-II) activity. Important for both skeletal development and hematopoiesis, through the formation of HS proteoglycans (HSPGs). Through the synthesis of HS, regulates postnatal pancreatic islet maturation and insulin secretion.</text>
    </comment>
    <comment type="function">
        <text evidence="19">Receptor for REG3A, REG3B and REG3G, induces the activation of downstream signaling pathways such as PI3K-AKT or RAS-RAF-MEK-ERK signaling pathway. Required for the function of REG3A in regulating keratinocyte proliferation and differentiation. Required for the inhibition of skin inflammation mediated by REG3A through the activation of PI3K-AKT-STAT3 pathway. Required for the function of REG3A and REG3G in glucose tolerance in pancreas. Expressed in microglia, is activated by nociceptor-derived REG3G in response to endotoxins, leading to the inhibition of kynurenine pathway to prevent endotoxic death.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>3-O-(beta-D-GlcA-(1-&gt;3)-beta-D-Gal-(1-&gt;3)-beta-D-Gal-(1-&gt;4)-beta-D-Xyl)-L-seryl-[protein] + UDP-N-acetyl-alpha-D-glucosamine = 3-O-(alpha-D-GlcNAc-(1-&gt;4)-beta-D-GlcA-(1-&gt;3)-beta-D-Gal-(1-&gt;3)-beta-D-Gal-(1-&gt;4)-beta-D-Xyl)-L-seryl-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16221"/>
            <dbReference type="Rhea" id="RHEA-COMP:12573"/>
            <dbReference type="Rhea" id="RHEA-COMP:12574"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:132093"/>
            <dbReference type="ChEBI" id="CHEBI:132104"/>
            <dbReference type="EC" id="2.4.1.223"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Glycan metabolism; heparan sulfate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="21">Homodimer; disulfide-linked. Interacts with REG3A.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the glycosyltransferase 47 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.223" evidence="22"/>
    <dbReference type="EMBL" id="EAAA01001383">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="EAAA01001384">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F7APA8"/>
    <dbReference type="FunCoup" id="F7APA8">
        <property type="interactions" value="222"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000015272"/>
    <dbReference type="Ensembl" id="ENSCINT00000015272.3">
        <property type="protein sequence ID" value="ENSCINP00000015272.3"/>
        <property type="gene ID" value="ENSCING00000007435.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156692"/>
    <dbReference type="HOGENOM" id="CLU_013906_3_0_1"/>
    <dbReference type="InParanoid" id="F7APA8"/>
    <dbReference type="OMA" id="KFMGSHT"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001888">
        <property type="term" value="F:glucuronyl-galactosyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016500">
        <property type="term" value="F:protein-hormone receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015012">
        <property type="term" value="P:heparan sulfate proteoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010838">
        <property type="term" value="P:positive regulation of keratinocyte proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051897">
        <property type="term" value="P:positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000033">
        <property type="entry name" value="Exostosin-like glycosyltransferase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004263">
        <property type="entry name" value="Exostosin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040911">
        <property type="entry name" value="Exostosin_GT47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015338">
        <property type="entry name" value="GT64_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48261">
        <property type="entry name" value="ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48261:SF4">
        <property type="entry name" value="EXOSTOSIN LIKE GLYCOSYLTRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03016">
        <property type="entry name" value="Exostosin_GT47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09258">
        <property type="entry name" value="Glyco_transf_64"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="26" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="17" id="KW-0464">Manganese</keyword>
    <keyword evidence="14 27" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 27" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 27" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="27">
        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="domain" description="Exostosin GT47" evidence="28">
        <location>
            <begin position="187"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase 64" evidence="29">
        <location>
            <begin position="659"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="26">
        <location>
            <begin position="76"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00050948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00058348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00058474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00061763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00066812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00074807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00079618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00083226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="Pfam" id="PF03016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="Pfam" id="PF09258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000015272.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="915" mass="104481" checksum="A099F56F16DED017" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>F7API7</accession>
    <name>F7API7_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ABC transmembrane type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">wild type</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002918">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000003293"/>
    <dbReference type="Ensembl" id="ENSCINT00000003293.3">
        <property type="protein sequence ID" value="ENSCINP00000003293.3"/>
        <property type="gene ID" value="ENSCING00000022381.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159126"/>
    <dbReference type="HOGENOM" id="CLU_1115443_0_0_1"/>
    <dbReference type="InParanoid" id="F7API7"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:monoatomic ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394">
        <property type="entry name" value="ATP-DEPENDENT PERMEASE MDL1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394:SF17">
        <property type="entry name" value="MITOCHONDRIAL POTASSIUM CHANNEL ATP-BINDING SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="172"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="9">
        <location>
            <begin position="121"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000003293.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="249" mass="27912" checksum="64A9601D0539B0FE" modified="2012-04-18" version="2">MHFQYISLLVGRHALKLRLPVNQTLLVSKSTLRNAKQILKAEFQKCTWLKNKNIKRLAQFTAISGTAIVSVSQALKLSGSHVICHGLEKPIEAENNVVAKEFSWTEFFNDYLLPEILPLSLAIMSALVGAFVNMKIPGALGELINILNQQAQTGLSNVADYMKGLREPVIQLMALYLCQGFTTFLYISLLSHLGENVACKLRTNLFKSFLQQDVAFHDEHKTGELIDRLTSDVQDFKSSFKLCVSXRNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>F7B5J8</accession>
    <name>F7B5J8_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Fibronectin type-III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">wild type</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01002139">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F7B5J8"/>
    <dbReference type="Ensembl" id="ENSCINT00000023186.2">
        <property type="protein sequence ID" value="ENSCINP00000022940.2"/>
        <property type="gene ID" value="ENSCING00000012238.2"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1590122_0_0_1"/>
    <dbReference type="InParanoid" id="F7B5J8"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050991">
        <property type="entry name" value="ECM_Regulatory_Proteins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46708:SF2">
        <property type="entry name" value="FIBRONECTIN TYPE-III DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46708">
        <property type="entry name" value="TENASCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003348171" description="Fibronectin type-III domain-containing protein" evidence="2">
        <location>
            <begin position="18"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="3">
        <location>
            <begin position="20"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000022940.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="168" mass="18639" checksum="57E8F05DDA62D597" modified="2011-07-27" version="1">MWRKIFVLFFFLERITALEAPVDLATVDISTHSLSVMWRNLDGEANWFKATISPLDEGETTQGVKYINESVVTWVDLVPGKNYTISVVAISGNGRISLATQIVASTKPMPPYRVRLSKFANRALSAHSFGTGRLMMFPVYGAVVYWDGPDSGHFDGYELTVNPPHGVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>H2XJG0</accession>
    <name>H2XJG0_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Rabaptin coiled-coil domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
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                <person name="Branno M."/>
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                <person name="Kobayashi K."/>
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                <person name="Makabe K.W."/>
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                <person name="Medina M."/>
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                <person name="Mount S."/>
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                <person name="Nishizaka S."/>
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                <person name="Shin-i T."/>
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                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
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                <person name="Richardson P."/>
                <person name="Lucas S."/>
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                <person name="Levine M."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
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            <strain evidence="2">wild type</strain>
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        </source>
    </evidence>
    <sequence length="221" mass="25334" checksum="F22C2065F8230715" modified="2012-04-18" version="1">MTKKLYKIIHTGHNLKHFVNQQTQNLFFTRYLETMVKFDRIETAWEAVSTVCHVYSLPPTMFNEGLLKAVLRDEKPEYIPLLCPNLSRKKQLEFMTVCLSAGKKLINHADKDTITQLKNIAEHCMTIANNESNTQQFNESINMSLHIIILSGDITALLNAVGMVLQNYKSAIISSETMTMCEEFSTKEGNAALIKKIRQLRKFYETQDETNAGAKREIKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>H2XKG5</accession>
    <name>H2XKG5_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="H2XKG5"/>
    <dbReference type="Ensembl" id="ENSCINT00000034019.1">
        <property type="protein sequence ID" value="ENSCINP00000030147.1"/>
        <property type="gene ID" value="ENSCING00000018892.1"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2941028_0_0_1"/>
    <dbReference type="InParanoid" id="H2XKG5"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000030147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="60" mass="7041" checksum="934CB22AD3682C57" modified="2012-04-18" version="1">MRIILKPRTFMKTSLLCLVKSWQFISGLLMVVLTFFSEHLYNPSARLKDNLRDKTSLLPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>H2XL49</accession>
    <name>H2XL49_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="H2XL49"/>
    <dbReference type="Ensembl" id="ENSCINT00000035634.1">
        <property type="protein sequence ID" value="ENSCINP00000030381.1"/>
        <property type="gene ID" value="ENSCING00000025074.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165633"/>
    <dbReference type="HOGENOM" id="CLU_001645_9_8_1"/>
    <dbReference type="InParanoid" id="H2XL49"/>
    <dbReference type="OMA" id="ICIHREF"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00047">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000185">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050348">
        <property type="entry name" value="Protein-Tyr_Phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134:SF562">
        <property type="entry name" value="PROTEIN-TYROSINE-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134">
        <property type="entry name" value="RECEPTOR-TYPE TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="5">
        <location>
            <begin position="1"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="6">
        <location>
            <begin position="101"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009580"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000030381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="234" mass="27337" checksum="73BEF28B08F4CC0E" modified="2012-04-18" version="1">MKNTVVDFWAMVWEQRCPTIVMLTNLTENGKVKCEKYWPDKNSVYKCGDIHIVTMEETMHGCYVIRKLQVSMLDKNKRTIVQYQFLTWGEHDAPTTTSGFFKFFDQINEGNDGNEYTVVHCSDGVGRTGSFIAFNFILSTMEQNQQVDVYDVIMQLRKQRPYMVQTLEQYIFLHKLLLEDIVLDDCEVNVDDINMVISQLDKTNHIGKTILQTEYENLDLIEPIKTQQTMAMKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>H2XLC2</accession>
    <name>H2XLC2_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">AP complex subunit beta</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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                <person name="Ueno K."/>
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                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
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                <person name="Wiley G.B."/>
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                <person name="Zeller R.W."/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
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                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
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                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
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                <person name="Putnam N."/>
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                <person name="Satake M."/>
                <person name="Terry A."/>
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                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
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                <person name="Makabe K.W."/>
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                <person name="Mochizuki Y."/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
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                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
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                <person name="Gelpke M."/>
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                <person name="Harafuji N."/>
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                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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                <person name="De Tomaso A."/>
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                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
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                <person name="Martinez D."/>
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                <person name="Putnam N."/>
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                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
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                <person name="Makabe K.W."/>
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                <person name="Mochizuki Y."/>
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                <person name="Nomoto H."/>
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                <person name="Shoguchi E."/>
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                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
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                <person name="Yagi K."/>
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                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
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                <person name="Doggett N."/>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
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                <person name="Chapman J."/>
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                <person name="De Tomaso A."/>
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                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
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                <person name="Meinertzhagen I.A."/>
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                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
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                <person name="DeSantis R."/>
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                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
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                <person name="Kobayashi M."/>
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                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
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                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
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                <person name="Lucas S."/>
                <person name="Kohara Y."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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    <reference evidence="3" key="3">
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the inositol 1,4,5-trisphosphate 5-phosphatase type II family.</text>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
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                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
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                <person name="Keys D.N."/>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
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                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
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                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
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                <person name="Branno M."/>
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                <person name="Makabe K.W."/>
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                <person name="Mount S."/>
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                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
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                <person name="Richardson P."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
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                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
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                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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        <property type="term" value="C:membrane attack complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044218">
        <property type="term" value="C:other organism cell membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031640">
        <property type="term" value="P:killing of cells of another organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001862">
        <property type="entry name" value="MAC_perforin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020864">
        <property type="entry name" value="MACPF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020863">
        <property type="entry name" value="MACPF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45742">
        <property type="entry name" value="COMPLEMENT COMPONENT C6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45742:SF8">
        <property type="entry name" value="FLOCCULATION PROTEIN FLO11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01823">
        <property type="entry name" value="MACPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00764">
        <property type="entry name" value="COMPLEMENTC9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00457">
        <property type="entry name" value="MACPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00279">
        <property type="entry name" value="MACPF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51412">
        <property type="entry name" value="MACPF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0180">Complement pathway</keyword>
    <keyword evidence="8" id="KW-0204">Cytolysis</keyword>
    <keyword evidence="13 16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0391">Immunity</keyword>
    <keyword evidence="7" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0473">Membrane attack complex</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="17" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-1052">Target cell membrane</keyword>
    <keyword evidence="15" id="KW-1053">Target membrane</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003578063" description="MACPF domain-containing protein" evidence="17">
        <location>
            <begin position="20"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="domain" description="MACPF" evidence="18">
        <location>
            <begin position="139"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="121"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000031443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="509" mass="57436" checksum="ED9DD35ED3007CF8" modified="2012-04-18" version="1">MFNYCFYILLLLLLKVSDNSRNNILQAAKETRDCMPPCATDCEWNFWSKYGKCGCKSKEQLRHRTVKQPSLFGGKSCDGAEEDSIPCKEPSKQCKNKKKKCGKKKFACKAGTSCIKNALVCNGDADCVDSSDELKCKKVKLTCGKETYPLIPNIQIVGGAFDITTLARSSEVLDNSLFNGVCNNVYSPDHKKHFRKPYNVASFSYQVNQTHFLHYSIFNCHQNPLTAKRACRNMQTARQSVSIYTFIKMTQMSQLVGHAAGGDNAKLEKMIKDGYSDDYKYFSVKTVITVAMFQMRDDNLLLNEDFEEDLNELHSWYDKVEYLRLLEKYGTHYYTEGTLGGQYKFIYKFRKADLVKADLTDTEQRQCLEDDAKIKLLGADVDINDRCNFNSKSFTNNAVQSVSFVDGGEANAVSSLSVNSDEKSLTEWTNSVIMHPAIVEYKIAPLSQLIDSRIIQGKKKKANMETALLHFMKKFEPIRCNSFNAGKVVKDGRECVCLCPAGLYGAGCE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>H2XPD0</accession>
    <name>H2XPD0_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Xanthine dehydrogenase/oxidase-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC100181207</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Mo-molybdopterin</name>
            <dbReference type="ChEBI" id="CHEBI:71302"/>
        </cofactor>
        <text evidence="12">Binds 1 Mo-molybdopterin (Mo-MPT) cofactor per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
        <text evidence="12">Binds 2 [2Fe-2S] clusters.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the xanthine dehydrogenase family.</text>
    </comment>
    <dbReference type="STRING" id="7719.ENSCINP00000031513"/>
    <dbReference type="Ensembl" id="ENSCINT00000030748.1">
        <property type="protein sequence ID" value="ENSCINP00000031513.1"/>
        <property type="gene ID" value="ENSCING00000019689.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166820"/>
    <dbReference type="HOGENOM" id="CLU_001681_1_0_1"/>
    <dbReference type="InParanoid" id="H2XPD0"/>
    <dbReference type="OMA" id="HWYWPKT"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000087">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.390.50:FF:000003">
        <property type="entry name" value="Aldehyde oxidase1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.365.10:FF:000001">
        <property type="entry name" value="Xanthine dehydrogenase oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.365.10:FF:000002">
        <property type="entry name" value="Xanthine dehydrogenase oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.120">
        <property type="entry name" value="[2Fe-2S]-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.50">
        <property type="entry name" value="Aldehyde oxidase/xanthine dehydrogenase, a/b hammerhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.365.10">
        <property type="entry name" value="Aldehyde oxidase/xanthine dehydrogenase, molybdopterin binding domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.50">
        <property type="entry name" value="CO dehydrogenase flavoprotein, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.43.10">
        <property type="entry name" value="Uridine Diphospho-n-acetylenolpyruvylglucosamine Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002888">
        <property type="entry name" value="2Fe-2S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036884">
        <property type="entry name" value="2Fe-2S-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000674">
        <property type="entry name" value="Ald_Oxase/Xan_DH_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036856">
        <property type="entry name" value="Ald_Oxase/Xan_DH_a/b_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016208">
        <property type="entry name" value="Ald_Oxase/xanthine_DH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008274">
        <property type="entry name" value="AldOxase/xan_DH_MoCoBD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046867">
        <property type="entry name" value="AldOxase/xan_DH_MoCoBD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037165">
        <property type="entry name" value="AldOxase/xan_DH_Mopterin-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005107">
        <property type="entry name" value="CO_DH_flav_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036683">
        <property type="entry name" value="CO_DH_flav_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016167">
        <property type="entry name" value="FAD-bd_PCMH_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002346">
        <property type="entry name" value="Mopterin_DH_FAD-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11908:SF132">
        <property type="entry name" value="ALDEHYDE OXIDASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11908">
        <property type="entry name" value="XANTHINE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01315">
        <property type="entry name" value="Ald_Xan_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03450">
        <property type="entry name" value="CO_deh_flav_C"/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
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                <person name="Huang W."/>
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                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
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                <person name="Doyle S."/>
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                <person name="Nomoto H."/>
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                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
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                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Makabe K.W."/>
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                <person name="Mochizuki Y."/>
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                <person name="Morishita T."/>
                <person name="Miura S."/>
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                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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            <strain evidence="5">wild type</strain>
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    <reference evidence="5" key="3">
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                <consortium name="Ensembl"/>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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            <location evidence="1">Nucleus</location>
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        <property type="molecule type" value="Genomic_DNA"/>
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<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
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        <name type="scientific">Ciona intestinalis</name>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
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                <person name="Oishi K."/>
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                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
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                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="7">wild type</strain>
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    <reference evidence="7" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <text evidence="3">Belongs to the SKI8 family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="ENSCING00000020437.1"/>
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    <dbReference type="KEGG" id="cin:100182087"/>
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        <property type="term" value="C:Cdc73/Paf1 complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        </source>
    </evidence>
    <sequence length="305" mass="33755" checksum="BBE374C5E6FB8E84" modified="2012-04-18" version="1">MSTQYSVLFKQEQAHDEDIWCCAWGKSEKDGTEHIVTGSLDDKVKCWKWVDEQLELKWILEGHQLGIVSVDINSSGTIAASSSLDAHIRLWDLDSGKQLRAIDGGTVETWTVAFSPDGNHIATGGQSGQILVINVDDGRTEFKLDTRGKFTLSIAYSDNGKYVACGAMDGIIKVFDVASQRLLHTLEGHAMPIRSLAFSPDSHRLVTASDDGQIKVYDVKNATFIQTLSGHSSWVHGVAFCHNNLHFASCSADKTVKIWDVSSHQCVNTFYDHRDQVWSCCYNNNGSKLVSVSDDKAIHIYDCPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>H2XPV1</accession>
    <name>H2XPV1_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">wild type</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2"/>
    <dbReference type="EMBL" id="EAAA01001383">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XPV1"/>
    <dbReference type="FunCoup" id="H2XPV1">
        <property type="interactions" value="21"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000031685"/>
    <dbReference type="Ensembl" id="ENSCINT00000036640.1">
        <property type="protein sequence ID" value="ENSCINP00000031685.1"/>
        <property type="gene ID" value="ENSCING00000019880.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000169786"/>
    <dbReference type="HOGENOM" id="CLU_001645_8_0_1"/>
    <dbReference type="InParanoid" id="H2XPV1"/>
    <dbReference type="OMA" id="NDENCCK"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00047">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000145">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase epsilon-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000062">
        <property type="entry name" value="Receptor-type tyrosine-protein phosphatase kappa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050348">
        <property type="entry name" value="Protein-Tyr_Phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134:SF562">
        <property type="entry name" value="PROTEIN-TYROSINE-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134">
        <property type="entry name" value="RECEPTOR-TYPE TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="5">
        <location>
            <begin position="30"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="6">
        <location>
            <begin position="207"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="5">
        <location>
            <begin position="320"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="6">
        <location>
            <begin position="492"/>
            <end position="564"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009580"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000031685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="584" mass="67383" checksum="0A5A6E92D3995CF8" modified="2012-04-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>H2XQU1</accession>
    <name>H2XQU1_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="H2XQU1"/>
    <dbReference type="Ensembl" id="ENSCINT00000034554.1">
        <property type="protein sequence ID" value="ENSCINP00000032025.1"/>
        <property type="gene ID" value="ENSCING00000019735.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00730000113953"/>
    <dbReference type="HOGENOM" id="CLU_1100566_0_0_1"/>
    <dbReference type="InParanoid" id="H2XQU1"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003577381" description="C-type lectin domain-containing protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000032025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="232" mass="26736" checksum="D01C2C8108BA1FED" modified="2012-04-18" version="1">MCLGLMVIISCWLTIIQACHYAEYLDRRKRSTTTTNSSSDSFDSNRLIYWFTVCMQNRSSLGSYDWGSVAEWTVTDSNWFLYTENSNCKDTEGVEETECWINRVSSFIDDPVASTLRSCIYEYSSYKYSSRNGSVSRKKRQTIPSPILPSMPPCPNVVRRLYRMALLEEIPRKKRRQTSMGVPEVPDFDIDAVSSFVENYTTCSECSRYNEGEAAAQCWTNRFDQLTKDNSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>H2XTQ0</accession>
    <name>H2XTQ0_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
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                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
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                <person name="Makabe K.W."/>
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                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
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                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
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                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
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                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
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                <person name="Richardson P."/>
                <person name="Lucas S."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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                <consortium name="Ensembl"/>
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        <subcellularLocation>
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            <topology evidence="1">Multi-pass membrane protein</topology>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="Ensembl" id="ENSCINT00000030762.1">
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    <reference evidence="7" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
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                <person name="Hino K."/>
                <person name="Imai K.S."/>
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                <person name="Kobayashi K."/>
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                <person name="Makabe K.W."/>
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                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
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                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="6" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="6">wild type</strain>
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    <reference evidence="6" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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    <reference evidence="6" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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            <location evidence="1">Golgi apparatus</location>
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        <text evidence="2">Belongs to the GKAP1 family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="entry name" value="G KINASE ANCHORING PROTEIN 1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="G KINASE-ANCHORING PROTEIN 1"/>
        <property type="match status" value="1"/>
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        <source>
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</entry>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Chapman J."/>
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                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01000486">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XWL7"/>
    <dbReference type="Ensembl" id="ENSCINT00000031864.1">
        <property type="protein sequence ID" value="ENSCINP00000034051.1"/>
        <property type="gene ID" value="ENSCING00000018309.1"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1829656_0_0_1"/>
    <dbReference type="InParanoid" id="H2XWL7"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="108"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="35"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="116"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000034051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="141" mass="14291" checksum="FAC8CF9A924B6E00" modified="2012-04-18" version="1">METDVLDLPATTESDVPLSVDERVDAKPVKVDAVAQEGSEGAEQSGEVKESDSAAAVEDEPMEQEIEGNTADVAEDTEVESGLGALVNNGGAIVDGEAEAIPADQSTEAAVAPIESTETPSGEQSETTEAAQTDSGIAEAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>H2XWM3</accession>
    <name>H2XWM3_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">wild type</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01003001">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XWM3"/>
    <dbReference type="STRING" id="7719.ENSCINP00000034057"/>
    <dbReference type="Ensembl" id="ENSCINT00000034340.1">
        <property type="protein sequence ID" value="ENSCINP00000034057.1"/>
        <property type="gene ID" value="ENSCING00000023562.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161826"/>
    <dbReference type="HOGENOM" id="CLU_551600_0_0_1"/>
    <dbReference type="InParanoid" id="H2XWM3"/>
    <dbReference type="OMA" id="LCTIHED"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:003372">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001360">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050541">
        <property type="entry name" value="LRR_TM_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24369">
        <property type="entry name" value="ANTIGEN BSP, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24369:SF215">
        <property type="entry name" value="PROTEIN WINDPIPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003578207" description="Ig-like domain-containing protein" evidence="5">
        <location>
            <begin position="19"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="392"/>
            <end position="495"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000034057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="495" mass="55460" checksum="8602311B2ECB8076" modified="2012-04-18" version="1">TKQTAMVCILFFVFLTLAAEGNMLVRACPQQGGACDCNSVTRSVTCTRLGSLTRRLPLGIPANTKVLTVNHSTLSRLGPTAFRNCYQLEVVDLSDNRLEDISQLAFQSLGRLRSLNLSDNRLRVVTEDTFLHLKRLEFLDLSRNRLIVLTPDNFKNLHNLKELKLSNNPTMCIAPSAFWGMKKLQVLELSKLGSHGIPKTLLTGLSRLQTLSLKACSLDNIPNQLLDRVQRSIVELDLSHNLINIMVYLNIMNIPITSRLFSLQNLYYLKTLNLRRTGLTLIESGALNLSNLVNIDLYGNNLSVMTVSAELERVQCTISFKQIYNFLLLNGCNESVFGDSMKTLRNVSLANNPWVCDCRLRWIIRSNFVTTTDRPVVCVQPPWMQGYNIRSPVDHSTLRVDSFSCKYRKSRETSLSLGSSMAPVTIKVVCKSDGFPKPKVRWVLPYTTALNDGVTQVSKDGSLHLHMVTPQQATGVYTCRSSNQFGGSEMSFVIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>H2XXC2</accession>
    <name>H2XXC2_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
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                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
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                <person name="Azumi K."/>
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                <person name="Hino K."/>
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                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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            <strain evidence="1">wild type</strain>
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    <reference evidence="1" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01001432">
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="H2XXC2"/>
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        <property type="component" value="Chromosome 2"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
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        <source>
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    </evidence>
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        <source>
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    <sequence length="55" mass="6403" checksum="7B4A1F70CD5BC15E" modified="2012-04-18" version="1">MFCFLTCFHRSTLTVLQTSYKVVFNNKRSHLPKSHANVMAFFVLLLPSCDCMNIF</sequence>
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    <accession>H2XXI7</accession>
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            <fullName evidence="4">Prefoldin subunit 5</fullName>
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        <name type="common">Transparent sea squirt</name>
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    <reference evidence="6" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
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                <person name="Oishi K."/>
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                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">wild type</strain>
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    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <comment type="function">
        <text evidence="3">Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins. Represses the transcriptional activity of MYC.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the prefoldin subunit alpha family.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="project" value="InterPro"/>
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</entry>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
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                <person name="Meinertzhagen I.A."/>
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                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
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                <person name="Doyle S."/>
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                <person name="Keys D.N."/>
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                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
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                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
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                <person name="Richardson P."/>
                <person name="Lucas S."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
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                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
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                <person name="Inaba K."/>
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            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">wild type</strain>
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            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    </evidence>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
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                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
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                <person name="Hastings K.E."/>
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                <person name="Kawashima T."/>
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                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
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                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
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                <person name="Zhang C."/>
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                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
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    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
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                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">wild type</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <text evidence="4">Ubiquitin-like protein involved in autophagic vesicle formation.</text>
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        <text evidence="4">Forms a conjugate with ATG5.</text>
    </comment>
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        <text evidence="4">Belongs to the ATG12 family.</text>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
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                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">wild type</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>AMP + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + adenine</text>
            <dbReference type="Rhea" id="RHEA:16609"/>
            <dbReference type="ChEBI" id="CHEBI:16708"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.4.2.7"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Purine metabolism; AMP biosynthesis via salvage pathway; AMP from adenine: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the purine/pyrimidine phosphoribosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.7" evidence="6"/>
    <dbReference type="EMBL" id="EAAA01001233">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002121449.1">
        <property type="nucleotide sequence ID" value="XM_002121413.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2Y0D5"/>
    <dbReference type="FunCoup" id="H2Y0D5">
        <property type="interactions" value="347"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000035369"/>
    <dbReference type="Ensembl" id="ENSCINT00000034775.1">
        <property type="protein sequence ID" value="ENSCINP00000035369.1"/>
        <property type="gene ID" value="ENSCING00000018906.1"/>
    </dbReference>
    <dbReference type="GeneID" id="100186031"/>
    <dbReference type="KEGG" id="cin:100186031"/>
    <dbReference type="GeneTree" id="ENSGT00390000017259"/>
    <dbReference type="HOGENOM" id="CLU_063339_3_2_1"/>
    <dbReference type="InParanoid" id="H2Y0D5"/>
    <dbReference type="OMA" id="QAYDLEY"/>
    <dbReference type="OrthoDB" id="363185at2759"/>
    <dbReference type="UniPathway" id="UPA00588">
        <property type="reaction ID" value="UER00646"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002055">
        <property type="term" value="F:adenine binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003999">
        <property type="term" value="F:adenine phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016208">
        <property type="term" value="F:AMP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006168">
        <property type="term" value="P:adenine salvage"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044209">
        <property type="term" value="P:AMP salvage"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000021">
        <property type="entry name" value="Adenine phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00004">
        <property type="entry name" value="Aden_phosphoribosyltr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005764">
        <property type="entry name" value="Ade_phspho_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050054">
        <property type="entry name" value="UPRTase/APRTase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01090">
        <property type="entry name" value="apt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002634">
        <property type="entry name" value="PRK02304.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002636">
        <property type="entry name" value="PRK02304.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32315">
        <property type="entry name" value="ADENINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32315:SF3">
        <property type="entry name" value="ADENINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11" id="KW-0660">Purine salvage</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="12">
        <location>
            <begin position="57"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00017366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000035369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="185" mass="20490" checksum="50CC47A4B88BBA70" modified="2012-04-18" version="1">MAKPDPRIEAIKARIQDFPDFPKPGILFRDIFPLFQHPSLCHDVVNVFYEHFVSKYPSTKVDAVLGLDARGFLFAPLLAEKFRANFVAVRKKGKLPGKVVSCDYTLEYGSAEVEIQNHAVSDGQNVIIVDDLLATGGTMNAACLLAKKLKLNIIQCQCVIALTGLNGEKKLPAGIDFYSIVQYEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>H2Y1V5</accession>
    <accession>A0A1W2WJF4</accession>
    <name>H2Y1V5_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Calcineurin B homologous protein 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC100178190</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">wild type</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the calcineurin regulatory subunit family. CHP subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01000762">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002128696.1">
        <property type="nucleotide sequence ID" value="XM_002128660.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2Y1V5"/>
    <dbReference type="FunCoup" id="H2Y1V5">
        <property type="interactions" value="70"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000035890"/>
    <dbReference type="Ensembl" id="ENSCINT00000034048.1">
        <property type="protein sequence ID" value="ENSCINP00000035890.1"/>
        <property type="gene ID" value="ENSCING00000019614.1"/>
    </dbReference>
    <dbReference type="GeneID" id="100178190"/>
    <dbReference type="KEGG" id="cin:100178190"/>
    <dbReference type="GeneTree" id="ENSGT00940000168326"/>
    <dbReference type="HOGENOM" id="CLU_061288_10_5_1"/>
    <dbReference type="InParanoid" id="H2Y1V5"/>
    <dbReference type="OMA" id="LKFAFRM"/>
    <dbReference type="OrthoDB" id="191686at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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                <person name="Goodstein D.M."/>
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                <person name="Huang W."/>
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                <person name="Martinez D."/>
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                <person name="Satake M."/>
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                <person name="Wada S."/>
                <person name="Zhang C."/>
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                <person name="Detter C."/>
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                <person name="Richardson P."/>
                <person name="Lucas S."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
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                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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                <consortium name="Ensembl"/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Putnam N."/>
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        <citation type="journal article" date="2006" name="Dev. Biol." volume="292" first="546" last="554">
            <title>Systematic analysis of embryonic expression profiles of zinc finger genes in Ciona intestinalis.</title>
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                <person name="Chiba T."/>
                <person name="Horii R."/>
                <person name="Yamada L."/>
                <person name="Kubo A."/>
                <person name="Miyamura D."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
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                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
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                <person name="Lemaire P."/>
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            <strain evidence="15">wild type</strain>
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            <authorList>
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        <text evidence="2">Belongs to the FAM10 family.</text>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2002-09" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Fort P.P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Mixed tissue</tissue>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="journal article" date="2003" name="Biol. Cell" volume="95" first="295" last="302">
            <title>Ascidians as a vertebrate-like model organism for physiological studies of Rho GTPase signaling.</title>
            <authorList>
                <person name="Philips A."/>
                <person name="Blein M."/>
                <person name="Robert A."/>
                <person name="Chambon J.P."/>
                <person name="Baghdiguian S."/>
                <person name="Weill M."/>
                <person name="Fort P."/>
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            <dbReference type="DOI" id="10.1016/S0248-4900(03)00052-2"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Mixed tissue</tissue>
        </source>
    </reference>
    <reference evidence="4" key="4">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
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            </authorList>
            <dbReference type="PubMed" id="18854010"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">wild type</strain>
        </source>
    </reference>
    <reference evidence="4" key="5">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAD48482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000023349.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="249" mass="27790" checksum="25270B0D0362EB95" modified="2003-10-01" version="1">MERIPNDVKGAFPEQQPKIKCVIVGDGGCGKTSMLVVYTEGEFPEVYTPTVFENYSREVELNRKKAVLTLWDTAGQEDYDRLRPLSYNNADIIIICYDVTNLSSFQNVEIRWSPEIRHFCPHTPTMLVACKTDLRSQVNSDSISNSMTRSASMATTLTTTGVDLTEMTSSTLSVNSVRAEDGTDRHDDQAIVTTEMGEKMSKHINVNHFVECSAKDGTNIEQVFKLAIECVIKREAKVGPKNCCCCLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-06-07" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1W3JEY8</accession>
    <accession>F6ZLM3</accession>
    <name>A0A1W3JEY8_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">60S acidic ribosomal protein P0</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100180640</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">wild type</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 6">Ribosomal protein P0 is the functional equivalent of E.coli protein L10.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the universal ribosomal protein uL10 family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01000834">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002128891.1">
        <property type="nucleotide sequence ID" value="XM_002128855.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1W3JEY8"/>
    <dbReference type="FunCoup" id="A0A1W3JEY8">
        <property type="interactions" value="519"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000008143"/>
    <dbReference type="Ensembl" id="ENSCINT00000008143.3">
        <property type="protein sequence ID" value="ENSCINP00000008143.3"/>
        <property type="gene ID" value="ENSCING00000000723.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100180640"/>
    <dbReference type="KEGG" id="cin:100180640"/>
    <dbReference type="eggNOG" id="KOG0815">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000017839"/>
    <dbReference type="InParanoid" id="A0A1W3JEY8"/>
    <dbReference type="OMA" id="DMNPFKL"/>
    <dbReference type="OrthoDB" id="10259902at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043009">
        <property type="term" value="P:chordate embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05795">
        <property type="entry name" value="Ribosomal_P0_L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1730:FF:000002">
        <property type="entry name" value="60S acidic ribosomal protein P0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.105.20:FF:000001">
        <property type="entry name" value="60S acidic ribosomal protein P0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.105.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050323">
        <property type="entry name" value="Ribosomal_protein_uL10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001790">
        <property type="entry name" value="Ribosomal_uL10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040637">
        <property type="entry name" value="Ribosomal_uL10-like_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043164">
        <property type="entry name" value="Ribosomal_uL10-like_insert_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043141">
        <property type="entry name" value="Ribosomal_uL10-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030670">
        <property type="entry name" value="uL10_eukaryotes"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45699">
        <property type="entry name" value="60S ACIDIC RIBOSOMAL PROTEIN P0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45699:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00428">
        <property type="entry name" value="Ribosomal_60s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00466">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17777">
        <property type="entry name" value="RL10P_insert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039087">
        <property type="entry name" value="L10E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160369">
        <property type="entry name" value="Ribosomal protein L10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL10-like insertion" evidence="8">
        <location>
            <begin position="111"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="280"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="284"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="296"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR039087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000008143.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="311" mass="33982" checksum="EDEB15F13D8C18F6" modified="2017-06-07" version="1">MPREDRKTWKANYFVKLINLLDEYPKCFIVGADNVSSKQMQQIRTSLREKAVVLMGKNTMMRKAIKGHLNNNPDLERLLPHIRGNVGLVFTKEDLIETRDLLLANKVAAPARAGAVAPNDVIVPAQNTGLGPEKTSFFQALAIPTKISRGTIEIISDVHLIKPGDKVGASDATLLSMLKIFPFTYGLEIEQVYDSGSVFSPEVLDITDDDIRRKFTNAVRNVASVCLAIGYPTVASAPHSIINGFKNILAVAAETEITFPEAEKMKAYLADPTAFAVAAAPAASDEKKEEAKPAEESEEESDDDMGFGLFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>F6PJS6</accession>
    <name>F6PJS6_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
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                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
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                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
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                <person name="Levine M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <text evidence="2">Belongs to the AB hydrolase superfamily. Epoxide hydrolase family.</text>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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        <location>
            <begin position="158"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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    </evidence>
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        <source>
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        <source>
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    </evidence>
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        <source>
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    <sequence length="414" mass="47386" checksum="E6D25B86CE057A8F" modified="2012-04-18" version="2">FLHAAAKLRENGILTCILTNNWVNDQQNTKGKFSRGMFYLKLYFNEVIESAREGIRKPDTEIYKLTCKKLGVVPTEVVFLDDIGLNLKSASQLGIRCIKVTNTNTAIKELESAVQMKLTAPKDKTAFPPASHPDSVTHSYINVTPKVKLHFVEAGEGPAIILLHGFPDFWYGWRNQIPALVQAGYRVIVPDQRGFGESSCPPRIQDYGQKYLCDDVLKIFMMKNPGSFDYQLYFQEPGVAEKEFELDVERSLTLILKGLEKSEDDTIKKEKSGFWSTSNVRARGGMLVGAPSVAVRSPYLTDYDMRYYVKNFKRTGFRGPLNWYRNYELNWSWMKRFAGRKIIIPALMVTASHDLVLKPEYSVGMEKRIPLLSRLHIERCSHWTMQEQPYKLNCGLIKWLDEVHGKLPLVTSKI</sequence>
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        <lineage>
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    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6PT25"/>
    <dbReference type="Ensembl" id="ENSCINT00000024136.2">
        <property type="protein sequence ID" value="ENSCINP00000023890.2"/>
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        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="321"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000023890.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="428" mass="47253" checksum="3DFF66F5CF3F5F95" modified="2012-04-18" version="2">MFAISCKSTDSYGSIDVTFQKPNVSDNGTIIGVNWDFTTVKQVIQITYCPDVVDSILTKPKHQNATYNEVAQVQCLSGLPLFKGAYGSLAMRKSPGRAITYTCGAHAEWEGPDVECWKGQTVDIQNSESRPFILALNTPTLFFCDVTGKDNYDVIMTVGTQRFSQKNNVAAILTPSVDDFNKTVECYSQRNISSKRQVPLWFLFPPQLLNRQTFVHYLCVGDASTAIVTFRSNPPITECGKVRVNKQGKSFTSFVLQSSGVTNFIKFIFPKLSKSDLGYYDVMVTSCEPVFSNKTIHIEIKECSTPVAVVTVHKPDSRITIGLSTVCACLVLLLLIAVTCIAVKRRNNAPHSSEQPRSQTPCRPAIPNASNSCNEYRQQQSADYCVISDRRGYVNMPLPGAQQSQNDPRFQGGASLDAGAYESYFIPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>F6QN34</accession>
    <accession>A0A1W2W9Y4</accession>
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        <recommendedName>
            <fullName evidence="8">Transporter</fullName>
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    <gene>
        <name evidence="11" type="primary">LOC100178767</name>
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    <organism evidence="11 12">
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    <reference evidence="12" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
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                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
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                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
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                <person name="Mount S."/>
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                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
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                <person name="Hawkins T."/>
                <person name="Richardson P."/>
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                <person name="Levine M."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
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                <person name="Lindquist E."/>
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                <person name="Ho I."/>
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                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
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                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
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                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
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                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
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                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
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                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
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                <person name="Nomoto H."/>
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                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
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                <person name="Doggett N."/>
                <person name="Glavina T."/>
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                <person name="Richardson P."/>
                <person name="Lucas S."/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
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                <person name="Hino K."/>
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                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
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                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">wild type</strain>
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    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
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                <person name="Putnam N."/>
                <person name="Rash S."/>
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                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
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                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
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                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
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                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">wild type</strain>
        </source>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Bifunctional enzyme that catalyzes the fourth and fifth sequential steps of CoA biosynthetic pathway. The fourth reaction is catalyzed by the phosphopantetheine adenylyltransferase, coded by the coaD domain; the fifth reaction is catalyzed by the dephospho-CoA kinase, coded by the coaE domain. May act as a point of CoA biosynthesis regulation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>(R)-4'-phosphopantetheine + ATP + H(+) = 3'-dephospho-CoA + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19801"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57328"/>
            <dbReference type="ChEBI" id="CHEBI:61723"/>
            <dbReference type="EC" id="2.7.7.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:19802"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>3'-dephospho-CoA + ATP = ADP + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18245"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57328"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.24"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:18246"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="17">Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 4/5.</text>
    </comment>
    <comment type="pathway">
        <text evidence="18">Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 5/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">In the central section; belongs to the eukaryotic CoaD family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.24" evidence="20"/>
    <dbReference type="EC" id="2.7.7.3" evidence="4"/>
    <dbReference type="EMBL" id="EAAA01002851">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6T7H6"/>
    <dbReference type="FunCoup" id="F6T7H6">
        <property type="interactions" value="586"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000003735"/>
    <dbReference type="Ensembl" id="ENSCINT00000003735.3">
        <property type="protein sequence ID" value="ENSCINP00000003735.3"/>
        <property type="gene ID" value="ENSCING00000001847.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00550000075078"/>
    <dbReference type="HOGENOM" id="CLU_027827_2_1_1"/>
    <dbReference type="InParanoid" id="F6T7H6"/>
    <dbReference type="OMA" id="TQCLQSY"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004140">
        <property type="term" value="F:dephospho-CoA kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004595">
        <property type="term" value="F:pantetheine-phosphate adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02022">
        <property type="entry name" value="DPCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000899">
        <property type="entry name" value="Bifunctional coenzyme A synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000089">
        <property type="entry name" value="Bifunctional coenzyme A synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00376">
        <property type="entry name" value="Dephospho_CoA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001977">
        <property type="entry name" value="Depp_CoAkinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00152">
        <property type="entry name" value="dephospho-CoA kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10695:SF46">
        <property type="entry name" value="BIFUNCTIONAL COENZYME A SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10695">
        <property type="entry name" value="DEPHOSPHO-COA KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01121">
        <property type="entry name" value="CoaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51219">
        <property type="entry name" value="DPCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cytidyltransferase-like" evidence="22">
        <location>
            <begin position="184"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00051310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00051912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00059677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00060565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00060696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00061673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00066359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00067394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000003735.3"/>
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    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="560" mass="61836" checksum="6374F50ED2843EA4" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F6TP42</accession>
    <name>F6TP42_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01002448">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="F6TP42"/>
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        <property type="protein sequence ID" value="ENSCINP00000029532.2"/>
        <property type="gene ID" value="ENSCING00000017447.2"/>
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        <property type="component" value="Chromosome 7"/>
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    <dbReference type="InterPro" id="IPR051075">
        <property type="entry name" value="SCF_subunit_WD-repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19872:SF7">
        <property type="entry name" value="F-BOX AND WD REPEAT DOMAIN CONTAINING PROTEIN 10B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19872">
        <property type="entry name" value="UBIQUITIN LIGASE SPECIFICITY FACTOR/HREP PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="87"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000029532.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="152" mass="17044" checksum="2265B0D25C40ECB0" modified="2012-04-18" version="2">MRALRGNSNCDPILSLCCYTNNLIGGDRIVVNTTSNILLLQFEPISWNYEAQDNKEDEKLFKVERQVRPLTCTQYPYTRACRSRLSLSSATSKTARSTSGSRPNTACSATFQSSWKRPQSCYTEPRSVHSRISSRCQSSMSCTSSATNTSKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>F6U8D6</accession>
    <accession>A0A1W2W2Q7</accession>
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        <submittedName>
            <fullName evidence="2">Alpha- and gamma-adaptin-binding protein p34</fullName>
        </submittedName>
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    <gene>
        <name evidence="2" type="primary">LOC100177805</name>
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    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01001500">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002122251.1">
        <property type="nucleotide sequence ID" value="XM_002122215.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6U8D6"/>
    <dbReference type="FunCoup" id="F6U8D6">
        <property type="interactions" value="128"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000007460"/>
    <dbReference type="Ensembl" id="ENSCINT00000007460.3">
        <property type="protein sequence ID" value="ENSCINP00000007460.3"/>
        <property type="gene ID" value="ENSCING00000003628.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100177805"/>
    <dbReference type="KEGG" id="cin:100177805"/>
    <dbReference type="GeneTree" id="ENSGT00390000007218"/>
    <dbReference type="HOGENOM" id="CLU_056826_0_0_1"/>
    <dbReference type="InParanoid" id="F6U8D6"/>
    <dbReference type="OMA" id="VTAFWKA"/>
    <dbReference type="OrthoDB" id="1741717at2759"/>
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        <property type="component" value="Chromosome 2"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019341">
        <property type="entry name" value="Alpha/Gamma-adaptin-bd_p34"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14659">
        <property type="entry name" value="ALPHA- AND GAMMA-ADAPTIN-BINDING PROTEIN P34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14659:SF1">
        <property type="entry name" value="ALPHA- AND GAMMA-ADAPTIN-BINDING PROTEIN P34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10199">
        <property type="entry name" value="Adaptin_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="174"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="174"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000007460.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="275" mass="31374" checksum="71BAB70C0FE0008D" modified="2012-04-18" version="2">MSDQILAILPTSGTSANKLIQDLQETENIKEHINGETYTWSLSNKYYMADVEVQVVKSQNLISEDFARKLEAVILLFDAKKTSDSLTAMKSWLPFIQTWECDIKLAVCARCCSVGEEDGEFIKREEVQLWCIDNGFELVELDPVLEDYDEYEDYGITRIRRAIGAHTWTHMKLKENEPKKIQGEPKTEKKDKDVAEPAVNSLSMEEQIDAMLSNSNDDDQDDADFEALFQKMHLFKQSAMSMPENSRKEYAEKVVSAFWKAIGGDEEELSANEDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>F6U9S1</accession>
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        <recommendedName>
            <fullName evidence="5">RCR-type E3 ubiquitin transferase</fullName>
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    </protein>
    <organism evidence="16 17">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Tunicata</taxon>
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            <taxon>Phlebobranchia</taxon>
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            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">wild type</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-threonine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-3-O-ubiquitinyl-L-threonine.</text>
            <dbReference type="EC" id="2.3.2.33"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Axon</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the RING-Cys relay (RCR) family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.33" evidence="5"/>
    <dbReference type="EMBL" id="EAAA01002830">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="Ensembl" id="ENSCINT00000007159.3">
        <property type="protein sequence ID" value="ENSCINP00000007159.3"/>
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    <dbReference type="InParanoid" id="F6U9S1"/>
    <dbReference type="OMA" id="DYYCDYC"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 9"/>
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    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008582">
        <property type="term" value="P:regulation of synaptic assembly at neuromuscular junction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="CDD" id="cd19799">
        <property type="entry name" value="Bbox2_MYCBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RING-H2_PHR"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="E3 ubiquitin-protein ligase MYCBP2 isoform X1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
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        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45943">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE MYCBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45943:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE MYCBP2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
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                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
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                <person name="Doyle S."/>
                <person name="Francino P."/>
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                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
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                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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                <person name="Inaba K."/>
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            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="11">wild type</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
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                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">wild type</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="10" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 8">Involved in the retrieval of endoplasmic reticulum membrane proteins from the early Golgi compartment.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the RER1 family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002669">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002125887.1">
        <property type="nucleotide sequence ID" value="XM_002125851.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6UUL4"/>
    <dbReference type="FunCoup" id="F6UUL4">
        <property type="interactions" value="1007"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000011250"/>
    <dbReference type="Ensembl" id="ENSCINT00000011250.3">
        <property type="protein sequence ID" value="ENSCINP00000011250.3"/>
        <property type="gene ID" value="ENSCING00000005442.3"/>
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    <dbReference type="GeneID" id="100178216"/>
    <dbReference type="KEGG" id="cin:100178216"/>
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    <dbReference type="HOGENOM" id="CLU_074889_0_0_1"/>
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        <property type="component" value="Chromosome 8"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
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        <property type="term" value="C:Golgi membrane"/>
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        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0006621">
        <property type="term" value="P:protein retention in ER lumen"/>
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        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
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        <property type="project" value="GO_Central"/>
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        <property type="entry name" value="Rer1"/>
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        <property type="entry name" value="PROTEIN RER1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PROTEIN RER1"/>
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        <property type="entry name" value="Rer1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="AtER_Rer1p"/>
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    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="26"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="52"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="119"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014112"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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            <dbReference type="PIRNR" id="PIRNR016013"/>
        </source>
    </evidence>
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            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000011250.3"/>
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    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    </evidence>
    <sequence length="188" mass="21929" checksum="BFD2F1FB70413C31" modified="2012-04-18" version="2">MNSEPPPTLFTTKIARIYQTWLDKTVPFVAVRWAVTLVSYLLYFIRIYIIQGWYVVTYALAIYHLNLFIAFLSPKVDPSIYNDDSDDEGPHLPTGSGEEFRPFIRRLPEFKFWYSGSKAILIAFVCTLFSAFNIPVFWPILVMYFIILFGVTMKRQIKHMIKYRYLPFTHGKTKYKGKEDTGKVVGPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>F6UW56</accession>
    <name>F6UW56_CIOIN</name>
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        <recommendedName>
            <fullName evidence="4">adenylate cyclase</fullName>
            <ecNumber evidence="4">4.6.1.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">wild type</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP = 3',5'-cyclic AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15389"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <dbReference type="EC" id="4.6.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.1" evidence="4"/>
    <dbReference type="EMBL" id="EAAA01001286">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000014863"/>
    <dbReference type="Ensembl" id="ENSCINT00000014863.3">
        <property type="protein sequence ID" value="ENSCINP00000014863.3"/>
        <property type="gene ID" value="ENSCING00000007249.3"/>
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    <dbReference type="HOGENOM" id="CLU_001072_2_5_1"/>
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        <property type="component" value="Chromosome 14"/>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="GO_Central"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="match status" value="2"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Adenylate cyclase type 9"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="2"/>
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        <property type="entry name" value="A/G_cyclase_CS"/>
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        <property type="entry name" value="Nucleotide_cyclase"/>
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        <property type="entry name" value="ADENYLATE CYCLASE TYPE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ADENYLATE CYCLASE TYPE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="2"/>
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        <property type="match status" value="2"/>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00452">
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        <property type="match status" value="1"/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
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                <person name="Wiley G.B."/>
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                <person name="Roe B.A."/>
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                <person name="Lindquist E."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="CHOLINE DEHYDROGENASE, MITOCHONDRIAL"/>
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</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>F6V6U3</accession>
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        <recommendedName>
            <fullName evidence="9">Peptidase S1 domain-containing protein</fullName>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
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            <taxon>Phlebobranchia</taxon>
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    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">wild type</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome 9"/>
    </dbReference>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Atrial natriuretic peptide-converting enzyme"/>
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        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Trypsin-like serine proteases"/>
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    </dbReference>
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        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
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        <property type="entry name" value="Peptidase_S1A"/>
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        <property type="entry name" value="Trypsin_dom"/>
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        <property type="entry name" value="TSP1_rpt"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 7" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 7" id="KW-0720">Serine protease</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003348622" description="Peptidase S1 domain-containing protein" evidence="8">
        <location>
            <begin position="24"/>
            <end position="356"/>
        </location>
    </feature>
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        <location>
            <begin position="101"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="356" mass="39955" checksum="B0E260689430A36E" modified="2012-04-18" version="2">MAGIIRFLFFVFLFAALELQVDSTLEQKDVVSHCENVKDCDLCVYDYEIEFVQGCAVCKCTSPIAREQRMATWKRWERWSECDRSCNFGTRTRVRRCRNGVVGGIGCIGPVFQNRRCRNKRCPLWFDANKGSLPWMVTLRRYPSFSFFCGGSIIDKNWILTAAHCVENKPESYRGILGNYFNKMVDEEETIVGFSSVHIHKSYNDNTLDNDIALLKVAEPIVFNDHVKPVCLPEYNAGVSYVPNTDVIISGWGTLKSNGALSNALQQAYVDIVSLEECGKRYSSVFAPSVMCAGILDKGGIDTCQGDSGGPLVDPNGNVQLGVVSWGRGCALAQYPGVYTLVSYYRRWLDNIRNNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F6VFI3</accession>
    <name>F6VFI3_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Right handed beta helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Nonaka M."/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="De Tomaso A."/>
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                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
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                <person name="Mochizuki Y."/>
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                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
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    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
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                <consortium name="Ensembl"/>
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    <dbReference type="Ensembl" id="ENSCINT00000028867.2">
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
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                <person name="Keys D.N."/>
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                <person name="Makabe K.W."/>
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                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
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    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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        <source>
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    </evidence>
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        <source>
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    <sequence length="64" mass="7447" checksum="CC0E11E667E75219" modified="2012-04-18" version="2">MNYHHYRRFNCVIVTSYVVPYIALCSVPVPAKFIHRSIRISYTMYYVAELPTSQCAISHAAHKQ</sequence>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
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                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="8">wild type</strain>
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    <reference evidence="8" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">wild type</strain>
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    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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    <sequence length="335" mass="38691" checksum="85AB90D49CD0989A" modified="2012-04-18" version="2">MKGLNYDIVRRSDAARRSSKRAEARLARKFNEAGGIEDEMRYHLANMKREQASLRRELQGLKFEYKKITKEANSKNGIIHKAHPGEVEHNDENGEREDKSKENPKSAGDYGKNYFGYHSSPGSHKSHNRTVREIEKSLECYFETSNNEAAEHEAKKKLKHQKKHNKTVQDKVETWKEKNTSRRRFSTTVPPNLRHLAFLRKELVNNDTNIEENEPNSPNVTTLPPLPKQSDEKKSPRVTSPAPNDVTSLERVVEVDATKPCEKRTSVVSSTNKTQKASDLVFDADLYAPYGKLRMAHRLPDFTKAFNEAKQARYVRTRHKREFERKMSVGEIFKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>F6VY59</accession>
    <accession>A0A1W2WI58</accession>
    <name>F6VY59_CIOIN</name>
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        <submittedName>
            <fullName evidence="5">Uncharacterized LOC100176859</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100176859</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">wild type</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01000240">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002131539.1">
        <property type="nucleotide sequence ID" value="XM_002131503.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6VY59"/>
    <dbReference type="STRING" id="7719.ENSCINP00000008244"/>
    <dbReference type="Ensembl" id="ENSCINT00000008244.3">
        <property type="protein sequence ID" value="ENSCINP00000008244.3"/>
        <property type="gene ID" value="ENSCING00000004006.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100176859"/>
    <dbReference type="KEGG" id="cin:100176859"/>
    <dbReference type="GeneTree" id="ENSGT00390000003348"/>
    <dbReference type="HOGENOM" id="CLU_038474_0_0_1"/>
    <dbReference type="InParanoid" id="F6VY59"/>
    <dbReference type="OMA" id="QQICFHL"/>
    <dbReference type="OrthoDB" id="377549at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040346">
        <property type="entry name" value="GEX1/Brambleberry"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33538">
        <property type="entry name" value="PROTEIN GAMETE EXPRESSED 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33538:SF2">
        <property type="entry name" value="PROTEIN GAMETE EXPRESSED 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014090289" evidence="4">
        <location>
            <begin position="23"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="239"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="269"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="320"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="449"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="169"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000008244.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="532" mass="60911" checksum="1B1CAA968B22A121" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>F6VZQ1</accession>
    <name>F6VZQ1_CIOIN</name>
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        <recommendedName>
            <fullName evidence="2">(S)-2-hydroxy-acid oxidase</fullName>
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    <organism evidence="12 13">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Tunicata</taxon>
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            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">wild type</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <reaction evidence="8">
            <text>2-hydroxyoctanoate + O2 = 2-oxooctanoate + H2O2</text>
            <dbReference type="Rhea" id="RHEA:67940"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:133514"/>
            <dbReference type="ChEBI" id="CHEBI:176689"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:67941"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a (2S)-2-hydroxycarboxylate + O2 = a 2-oxocarboxylate + H2O2</text>
            <dbReference type="Rhea" id="RHEA:16789"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:35179"/>
            <dbReference type="ChEBI" id="CHEBI:58123"/>
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        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:16790"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.</text>
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    <dbReference type="EC" id="1.1.3.15" evidence="2"/>
    <dbReference type="EMBL" id="EAAA01001931">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="FunCoup" id="F6VZQ1">
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    <dbReference type="Ensembl" id="ENSCINT00000003109.3">
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        <property type="gene ID" value="ENSCING00000001562.3"/>
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    <dbReference type="GeneTree" id="ENSGT00390000018717"/>
    <dbReference type="HOGENOM" id="CLU_020639_6_1_1"/>
    <dbReference type="InParanoid" id="F6VZQ1"/>
    <dbReference type="OMA" id="RIWFRPK"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 4"/>
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    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0003973">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
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        <property type="project" value="InterPro"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="hydroxyacid oxidase 2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Aldolase_TIM"/>
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    <dbReference type="InterPro" id="IPR012133">
        <property type="entry name" value="Alpha-hydoxy_acid_DH_FMN"/>
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    <dbReference type="InterPro" id="IPR000262">
        <property type="entry name" value="FMN-dep_DH"/>
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    <dbReference type="InterPro" id="IPR037396">
        <property type="entry name" value="FMN_HAD"/>
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    <dbReference type="InterPro" id="IPR008259">
        <property type="entry name" value="FMN_hydac_DH_AS"/>
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    <dbReference type="PANTHER" id="PTHR10578:SF107">
        <property type="entry name" value="2-HYDROXYACID OXIDASE 1"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR10578">
        <property type="entry name" value="S -2-HYDROXY-ACID OXIDASE-RELATED"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Al-hdrx_acd_dh"/>
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    </dbReference>
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        <location>
            <begin position="1"/>
            <end position="354"/>
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    <feature type="active site" description="Proton acceptor" evidence="9">
        <location>
            <position position="259"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
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        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>FMN</name>
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        </ligand>
    </feature>
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        <location>
            <position position="129"/>
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            <position position="157"/>
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        <location>
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        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
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        <location>
            <position position="235"/>
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        <ligand>
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            <position position="259"/>
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        <ligand>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00013087"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00024042"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00029325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000138-1"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000138-2"/>
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    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000003109.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="354" mass="38733" checksum="FBDB01D50A98F591" modified="2012-04-18" version="2">MSAPVSVKDYENSAREKLPKSVWDYYSSGANNEQTLSDNCNAFSRYRLRPHVLNDVSKVNLGSSVLGTPIDFPVCIASTAMNKMAHPTGEIAVVKAAESMKIGYMQSTWATTSVEDITAAAPGAIRWLQLYIYKNREVTKQLVQRAERLGYQGIFLTVDTPILGKRYKDVKNNFSLPSHLSLENFKALDLKELHTVDGENGSGLAQMVAALIDPSLQWSDIAWLKTITSMPIVLKGIITGEMAKRAVKENVAGILVSNHGARQLDGVPATIDALREIVQAVDGKCEVYLDGGVRNGTDVIKAIAFGAKAVFIGRPVLWGLAHNGQEGVRHVLKMLREEFKTALQLMGCTSIEEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>F6W5S5</accession>
    <name>F6W5S5_CIOIN</name>
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        <recommendedName>
            <fullName evidence="6">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
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            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">wild type</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="EAAA01002357">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Ensembl" id="ENSCINT00000016072.3">
        <property type="protein sequence ID" value="ENSCINP00000016072.3"/>
        <property type="gene ID" value="ENSCING00000007855.3"/>
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    <dbReference type="OMA" id="KSEELPW"/>
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        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Inorganic phosphate cotransporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000452">
        <property type="entry name" value="sialin-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050382">
        <property type="entry name" value="MFS_Na/Anion_cotransporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11662:SF399">
        <property type="entry name" value="FI19708P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11662">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 17"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="101"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="127"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="167"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="198"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="301"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="335"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="360"/>
            <end position="378"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="390"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="432"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="15"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000016072.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="458" mass="50067" checksum="E9E5903CCC9AEA6C" modified="2012-04-18" version="2">GCYIKARYVVTIMAALGSVNLYLLRNSLNIALISMVGVSNSSISEKNQNNTASLLTTAVVCPRNQEGEFDWSLAKQGLLLSCYWYSYTLSNIPGAWLAQQFGIRITLGVTSLLSAILTFFVPPAARLSFGLLVALRITMGLLQGVTLPTIMQSIGTWSPPCENTRHIALSFTGVASGNVVAFTFAGFIISYFGWECTFYAAGGVGCLWSIAWFLLIHDTPLKHPRISKQESSYITKSLSDAASVETKTVPWRKILTSKPYWSTVVPHLAENWASAITHSWVPQYIANVLGFSIENVGILSSLPYGLNMFSLLASGFLSDFILIKTKIRKTLLRKVFTSIGMGGTVLSFLVLPSFGCNHTLAIVLLCTSYMFVGCNCKYNAGYRVALVDMAASYSGILYSISNAMTCVVTLFATQLVGVMLGDGQSLPYWRTVFYVTSAVSAFGLLVFLWFGTCELQPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>F6WHS8</accession>
    <name>F6WHS8_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">Protein HIRA</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">wild type</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 12">Required for replication-independent chromatin assembly and for the periodic repression of histone gene transcription during the cell cycle.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the WD repeat HIR1 family.</text>
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    <dbReference type="EMBL" id="EAAA01000627">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="F6WHS8"/>
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        <property type="interactions" value="528"/>
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    <dbReference type="STRING" id="7719.ENSCINP00000015981"/>
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        <property type="protein sequence ID" value="ENSCINP00000015981.3"/>
        <property type="gene ID" value="ENSCING00000007804.3"/>
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    <dbReference type="GeneTree" id="ENSGT00550000074919"/>
    <dbReference type="InParanoid" id="F6WHS8"/>
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        <property type="component" value="Chromosome 10"/>
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    <dbReference type="GO" id="GO:0000785">
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0000417">
        <property type="term" value="C:HIR complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
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        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
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        <property type="term" value="P:regulation of DNA-templated transcription"/>
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        <property type="project" value="InterPro"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    <feature type="repeat" description="WD" evidence="11">
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            <begin position="130"/>
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    <feature type="repeat" description="WD" evidence="11">
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            <begin position="173"/>
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    <feature type="domain" description="Protein HIRA-like C-terminal" evidence="14">
        <location>
            <begin position="770"/>
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        <location>
            <begin position="479"/>
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        <location>
            <begin position="484"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
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        <source>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00021597"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>F6WM93</accession>
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        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Ciona intestinalis</name>
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            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
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    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
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    <comment type="similarity">
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        <property type="project" value="GO_Central"/>
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        <property type="term" value="F:D-glucose:sodium symporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="Gene3D" id="1.20.1730.10">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038377">
        <property type="entry name" value="Na/Glc_symporter_sf"/>
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    <dbReference type="InterPro" id="IPR001734">
        <property type="entry name" value="Na/solute_symporter"/>
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    <dbReference type="NCBIfam" id="TIGR00813">
        <property type="entry name" value="sss"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11819:SF196">
        <property type="entry name" value="SODIUM_GLUCOSE COTRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11819">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 5"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00474">
        <property type="entry name" value="SSF"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
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    <proteinExistence type="inferred from homology"/>
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            <end position="56"/>
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    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="77"/>
            <end position="103"/>
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    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="109"/>
            <end position="131"/>
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    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
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        <source>
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        <source>
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            <dbReference type="SAM" id="Phobius"/>
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</entry>
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        <recommendedName>
            <fullName evidence="4">Olfactomedin-like domain-containing protein</fullName>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
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                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
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                <person name="Inaba K."/>
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                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
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                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
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                <person name="Inaba K."/>
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            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="5">wild type</strain>
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    <reference evidence="5" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Hastings K.E."/>
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                <person name="Meinertzhagen I.A."/>
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                <person name="Satake M."/>
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                <person name="Yamada L."/>
                <person name="Wang H.G."/>
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                <person name="Azumi K."/>
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                <person name="Hayashi H."/>
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                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
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                <person name="Tassy O."/>
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                <person name="Richardson P."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="207" mass="23175" checksum="63CD504981E881D2" modified="2012-04-18" version="2">YQKLVKQEDCCGTVKCVCRPCPQLPTCPFNHSPIDTIYDPTCNCPVRNCVRNYCPNGKPVGSTWKVNNCTTCKCEDVNLVTCGNKTCPSFDTNCPIEYVEWDSEHCCKKCNEPKLENCHVKNDTAILSVDDCESVIPVSITNCSGRCTSNSRYVKNKLQVDCQCCTPKQTLLLNIQMKCTNGTTYTHKHTQITSCKCSSSNCETMLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>F6XIQ9</accession>
    <accession>A0A1W2W7S1</accession>
    <name>F6XIQ9_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Aspartate aminotransferase</fullName>
            <ecNumber evidence="10">2.6.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100179315</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">wild type</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:21824"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="EC" id="2.6.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4 10">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="10">In eukaryotes there are cytoplasmic, mitochondrial and chloroplastic isozymes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.1" evidence="10"/>
    <dbReference type="EMBL" id="EAAA01001505">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002126101.1">
        <property type="nucleotide sequence ID" value="XM_002126065.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6XIQ9"/>
    <dbReference type="FunCoup" id="F6XIQ9">
        <property type="interactions" value="290"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000007609"/>
    <dbReference type="Ensembl" id="ENSCINT00000007609.3">
        <property type="protein sequence ID" value="ENSCINP00000007609.3"/>
        <property type="gene ID" value="ENSCING00000003669.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100179315"/>
    <dbReference type="KEGG" id="cin:100179315"/>
    <dbReference type="GeneTree" id="ENSGT00950000183082"/>
    <dbReference type="HOGENOM" id="CLU_032440_1_2_1"/>
    <dbReference type="InParanoid" id="F6XIQ9"/>
    <dbReference type="OMA" id="VGACTIV"/>
    <dbReference type="OrthoDB" id="6752799at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004069">
        <property type="term" value="F:L-aspartate:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006533">
        <property type="term" value="P:L-aspartate catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000026">
        <property type="entry name" value="Aspartate aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000001">
        <property type="entry name" value="Aspartate aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000160">
        <property type="entry name" value="Similar to aspartate aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000796">
        <property type="entry name" value="Asp_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006719">
        <property type="entry name" value="PRK09257.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11879">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11879:SF22">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00799">
        <property type="entry name" value="TRANSAMINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="11">
        <location>
            <begin position="55"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000007609.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="425" mass="46705" checksum="1405CCBE8036C411" modified="2012-04-18" version="2">MSLTINKNICSLLQKCSVGHRSLNRTGSWWSNVEMGPPDPILGVTEAFKRDTNPKKMNLGVGAYRDDQGKPYVLPTVKKAELAIQGLDKEYLGITGLPAFTNAAAELAFGAGNTVLTDKRNVTVQGISGTGSLRIGANFLNKFFTSNKAIWLPTPSWGNHVPIFKHAGLDVEWYRYYKPSTCGFDAEGAMEDLNKIPENSIVLFHACAHNPTGVDPKPENWKEMSSICKKRNLLPFFDMAYQGFASGDINKDASAMRLFVAEGHNVILAQSFAKNMGLYGERAGAFTVVCADQEEAARVESQIKILIRPMYSNPPCNGARIASTVLTTPELREQWLVEVKGMADRIISMRQQLVDNLKKEGSTRDWSHITDQIGMFCYTGLNPDQVGDLTKNHSVYLTKDGRISVAGVASGNVGYLAHAIHQVTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>F6XUV4</accession>
    <name>F6XUV4_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA (cytosine-5)-methyltransferase 1</fullName>
            <ecNumber evidence="2">2.1.1.37</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.37" evidence="2"/>
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        <name type="scientific">Ciona intestinalis</name>
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            <taxon>Phlebobranchia</taxon>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">wild type</strain>
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    <reference evidence="6" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <text evidence="2">Belongs to the APC3/CDC27 family.</text>
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    <dbReference type="Pfam" id="PF13174">
        <property type="entry name" value="TPR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="4">
        <location>
            <begin position="557"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="4">
        <location>
            <begin position="591"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="4">
        <location>
            <begin position="625"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="4">
        <location>
            <begin position="727"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="237"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="323"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="772"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="237"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="348"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="361"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="783"/>
            <end position="800"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00039307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000015343.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="823" mass="92330" checksum="3A7CBA6AECDEA9A8" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-10-19" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>F6Y089</accession>
    <name>ITPA_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Inosine triphosphate pyrophosphatase</fullName>
            <shortName evidence="1">ITPase</shortName>
            <shortName evidence="1">Inosine triphosphatase</shortName>
            <ecNumber evidence="1">3.6.1.66</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Non-canonical purine NTP pyrophosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Non-standard purine NTP pyrophosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Nucleoside-triphosphate diphosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Nucleoside-triphosphate pyrophosphatase</fullName>
            <shortName evidence="1">NTPase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">XTP/dITP diphosphatase</fullName>
        </alternativeName>
    </protein>
    <organism>
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as inosine triphosphate (ITP), deoxyinosine triphosphate (dITP) or xanthosine 5'-triphosphate (XTP) to their respective monophosphate derivatives. The enzyme does not distinguish between the deoxy- and ribose forms. Probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ITP + H2O = IMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:29399"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
            <dbReference type="EC" id="3.6.1.66"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:29400"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>dITP + H2O = dIMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28342"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61194"/>
            <dbReference type="ChEBI" id="CHEBI:61382"/>
            <dbReference type="EC" id="3.6.1.66"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:28343"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>XTP + H2O = XMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28610"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:61314"/>
            <dbReference type="EC" id="3.6.1.66"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:28611"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="1">Binds 1 divalent metal cation per subunit; can use either Mg(2+) or Mn(2+).</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the HAM1 NTPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.66" evidence="1"/>
    <dbReference type="RefSeq" id="XP_002131447.1">
        <property type="nucleotide sequence ID" value="XM_002131411.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6Y089"/>
    <dbReference type="SMR" id="F6Y089"/>
    <dbReference type="FunCoup" id="F6Y089">
        <property type="interactions" value="625"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000025829"/>
    <dbReference type="Ensembl" id="ENSCINT00000026075.2">
        <property type="protein sequence ID" value="ENSCINP00000025829.2"/>
        <property type="gene ID" value="ENSCING00000014230.2"/>
    </dbReference>
    <dbReference type="GeneID" id="100180887"/>
    <dbReference type="KEGG" id="cin:100180887"/>
    <dbReference type="eggNOG" id="KOG3222">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015399"/>
    <dbReference type="HOGENOM" id="CLU_082080_1_1_1"/>
    <dbReference type="InParanoid" id="F6Y089"/>
    <dbReference type="OMA" id="YDPIFQP"/>
    <dbReference type="OrthoDB" id="6288734at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035870">
        <property type="term" value="F:dITP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036220">
        <property type="term" value="F:ITP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047429">
        <property type="term" value="F:nucleoside triphosphate diphosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036222">
        <property type="term" value="F:XTP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009204">
        <property type="term" value="P:deoxyribonucleoside triphosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009143">
        <property type="term" value="P:nucleoside triphosphate catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00515">
        <property type="entry name" value="HAM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.950.10:FF:000003">
        <property type="entry name" value="Inosine triphosphate pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03148">
        <property type="entry name" value="HAM1_NTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027502">
        <property type="entry name" value="ITPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029001">
        <property type="entry name" value="ITPase-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002637">
        <property type="entry name" value="RdgB/HAM1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00042">
        <property type="entry name" value="RdgB/HAM1 family non-canonical purine NTP pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11067:SF9">
        <property type="entry name" value="INOSINE TRIPHOSPHATE PYROPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11067">
        <property type="entry name" value="INOSINE TRIPHOSPHATE PYROPHOSPHATASE/HAM1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01725">
        <property type="entry name" value="Ham1p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52972">
        <property type="entry name" value="ITPase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0546">Nucleotide metabolism</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000413105" description="Inosine triphosphate pyrophosphatase">
        <location>
            <begin position="1"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="11"/>
            <end position="16"/>
        </location>
        <ligand>
            <name>ITP</name>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>ITP</name>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="69"/>
            <end position="70"/>
        </location>
        <ligand>
            <name>ITP</name>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>ITP</name>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="146"/>
            <end position="149"/>
        </location>
        <ligand>
            <name>ITP</name>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>ITP</name>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="174"/>
            <end position="175"/>
        </location>
        <ligand>
            <name>ITP</name>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03148"/>
        </source>
    </evidence>
    <sequence length="192" mass="21268" checksum="817F212104612C17" modified="2011-07-27" version="1">MASRKTISFVTGNKNKLKEVQQFLHGSSSINITSVPLDLPEYQGEPDDVSKQKCAEASKQLSGPVLIEDTCLCFNAMGGLPGPYVKWFLEKLGPEGIYKMLDGWEDKSGYALCTFAYSNGLQGDDVLLFRGKCEGTIVPPRGPRTFGWDPCFQPNGFNETYAEMSSELKNSISHRGKALEALSEYFKEKNCD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>F6Y8C3</accession>
    <name>F6Y8C3_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9 10">RNA cytidine acetyltransferase</fullName>
            <ecNumber evidence="10">2.3.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">18S rRNA cytosine acetyltransferase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">wild type</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">RNA cytidine acetyltransferase with specificity toward both 18S rRNA and tRNAs. Catalyzes the formation of N(4)-acetylcytidine (ac4C) in 18S rRNA. Required for early nucleolar cleavages of precursor rRNA at sites A0, A1 and A2 during 18S rRNA synthesis. Catalyzes the formation of ac4C in serine and leucine tRNAs. Requires a tRNA-binding adapter protein for full tRNA acetyltransferase activity but not for 18S rRNA acetylation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a cytidine in 18S rRNA + acetyl-CoA + ATP + H2O = an N(4)-acetylcytidine in 18S rRNA + ADP + phosphate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:51424"/>
            <dbReference type="Rhea" id="RHEA-COMP:13575"/>
            <dbReference type="Rhea" id="RHEA-COMP:13576"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:74900"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a cytidine in tRNA + acetyl-CoA + ATP + H2O = an N(4)-acetylcytidine in tRNA + ADP + phosphate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53876"/>
            <dbReference type="Rhea" id="RHEA-COMP:13670"/>
            <dbReference type="Rhea" id="RHEA-COMP:13671"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:74900"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
            <location evidence="1 10">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the RNA cytidine acetyltransferase family. NAT10 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="10"/>
    <dbReference type="EMBL" id="EAAA01001951">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="EAAA01001952">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6Y8C3"/>
    <dbReference type="FunCoup" id="F6Y8C3">
        <property type="interactions" value="799"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000007371"/>
    <dbReference type="Ensembl" id="ENSCINT00000007371.3">
        <property type="protein sequence ID" value="ENSCINP00000007371.3"/>
        <property type="gene ID" value="ENSCING00000003567.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000009140"/>
    <dbReference type="HOGENOM" id="CLU_004652_0_0_1"/>
    <dbReference type="InParanoid" id="F6Y8C3"/>
    <dbReference type="OMA" id="HLHYIMS"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990883">
        <property type="term" value="F:18S rRNA cytidine N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051392">
        <property type="term" value="F:tRNA cytidine N4-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904812">
        <property type="term" value="P:rRNA acetylation involved in maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051391">
        <property type="term" value="P:tRNA acetylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002101">
        <property type="term" value="P:tRNA wobble cytosine modification"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11040:FF:000006">
        <property type="entry name" value="RNA cytidine acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002218">
        <property type="entry name" value="tRNA(Met) cytidine acetyltransferase TmcA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03211">
        <property type="entry name" value="RNA_acetyltr_Nat10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033688">
        <property type="entry name" value="NAT10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007807">
        <property type="entry name" value="TcmA/NAT10_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032672">
        <property type="entry name" value="TmcA/NAT10/Kre33"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013562">
        <property type="entry name" value="TmcA/NAT10_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027992">
        <property type="entry name" value="tRNA_bind_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10925">
        <property type="entry name" value="N-ACETYLTRANSFERASE 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10925:SF5">
        <property type="entry name" value="RNA CYTIDINE ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13718">
        <property type="entry name" value="GNAT_acetyltr_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05127">
        <property type="entry name" value="NAT10_TcmA_helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08351">
        <property type="entry name" value="TmcA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13725">
        <property type="entry name" value="tRNA_bind_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 10" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="11">
        <location>
            <begin position="551"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="286"/>
            <end position="295"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="461"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="622"/>
            <end position="624"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="629"/>
            <end position="635"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="721"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00068357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000007371.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="956" mass="107159" checksum="647B392E37CF1ADD" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>F6YCF6</accession>
    <name>F6YCF6_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ETS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
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                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
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                <person name="Kawashima T."/>
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                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
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                <person name="Mochizuki Y."/>
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                <person name="Zhang C."/>
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                <person name="Richardson P."/>
                <person name="Lucas S."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Ueno K."/>
                <person name="Yamada L."/>
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                <person name="Wiley G.B."/>
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                <person name="Lemaire P."/>
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            <authorList>
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                <person name="Richardson P."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">wild type</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17 18">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cytoplasm</location>
            <location evidence="1 7">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the protein-tyrosine phosphatase family. Non-receptor class subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="7"/>
    <dbReference type="EMBL" id="EAAA01001336">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6YGI4"/>
    <dbReference type="FunCoup" id="F6YGI4">
        <property type="interactions" value="95"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000008730"/>
    <dbReference type="Ensembl" id="ENSCINT00000008730.3">
        <property type="protein sequence ID" value="ENSCINP00000008730.3"/>
        <property type="gene ID" value="ENSCING00000004230.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166146"/>
    <dbReference type="HOGENOM" id="CLU_001645_7_0_1"/>
    <dbReference type="InParanoid" id="F6YGI4"/>
    <dbReference type="OMA" id="MIRECRH"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008092">
        <property type="term" value="F:cytoskeletal protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13189">
        <property type="entry name" value="FERM_C_PTPN4_PTPN3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17100">
        <property type="entry name" value="FERM_F1_PTPN3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06706">
        <property type="entry name" value="PDZ_PTPN3-4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14541">
        <property type="entry name" value="PTPc-N3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000002">
        <property type="entry name" value="Band 4.1-like protein 5 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000039">
        <property type="entry name" value="Tyrosine-protein phosphatase non-receptor type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.10:FF:000003">
        <property type="entry name" value="Tyrosine-protein phosphatase non-receptor type 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019749">
        <property type="entry name" value="Band_41_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019747">
        <property type="entry name" value="FERM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000299">
        <property type="entry name" value="FERM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018979">
        <property type="entry name" value="FERM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018980">
        <property type="entry name" value="FERM_PH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041783">
        <property type="entry name" value="PTPN3/4_FERM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012151">
        <property type="entry name" value="Tyr_Pase_non-rcpt_typ-3/4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45706">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45706:SF4">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09380">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00373">
        <property type="entry name" value="FERM_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09379">
        <property type="entry name" value="FERM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000927">
        <property type="entry name" value="Tyr-Ptase_nr3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00935">
        <property type="entry name" value="BAND41"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00295">
        <property type="entry name" value="B41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01196">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47031">
        <property type="entry name" value="Second domain of FERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00660">
        <property type="entry name" value="FERM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50057">
        <property type="entry name" value="FERM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 7" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FERM" evidence="13">
        <location>
            <begin position="36"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="14">
        <location>
            <begin position="554"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="11">
        <location>
            <begin position="686"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="12">
        <location>
            <begin position="870"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="358"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="382"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphocysteine intermediate" evidence="8">
        <location>
            <position position="894"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="862"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="894"/>
            <end position="900"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="938"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000927-1"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000927-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000008730.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="987" mass="112234" checksum="9AC0CCBC877355FE" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>F6YHW9</accession>
    <name>F6YHW9_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">wild type</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01002336">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6YHW9"/>
    <dbReference type="Ensembl" id="ENSCINT00000022970.2">
        <property type="protein sequence ID" value="ENSCINP00000022724.2"/>
        <property type="gene ID" value="ENSCING00000012067.2"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1849560_0_0_1"/>
    <dbReference type="InParanoid" id="F6YHW9"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000022724.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="139" mass="15912" checksum="1739272C36BEA8D4" modified="2011-07-27" version="1">MYPDAVSESKDVLEFLRTSSDKKDGILDLLKKFSKLFAEKSSVQWTEGIREVLVRIFVILEACVSYPDLEDMDYLSKEDVQNMFFSILNHVECEVDCLQSKNKIEGCGLTVLGKHIAFLFQAVSYRQYFDTSPKFTSAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>F6YNR7</accession>
    <name>F6YNR7_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Disease resistance R13L4/SHOC-2-like LRR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">wild type</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01002646">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6YNR7"/>
    <dbReference type="STRING" id="7719.ENSCINP00000009123"/>
    <dbReference type="Ensembl" id="ENSCINT00000009123.3">
        <property type="protein sequence ID" value="ENSCINP00000009123.3"/>
        <property type="gene ID" value="ENSCING00000004411.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166939"/>
    <dbReference type="HOGENOM" id="CLU_000288_18_15_1"/>
    <dbReference type="InParanoid" id="F6YNR7"/>
    <dbReference type="OMA" id="EVINMEH"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001339">
        <property type="entry name" value="Leucine-rich repeat protein SHOC-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF1">
        <property type="entry name" value="RAS SUPPRESSOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="3">
        <location>
            <begin position="7"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000009123.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="151" mass="16758" checksum="5A7F1F3227C5EA86" modified="2012-04-18" version="2">MVSNNLLRKIPGSIGQLTNLQCLDLEENNLESLPSEIEHLTQLRKLKLQGNKLTVLPRGLGHLSNLTILAVGENQMRDLPNEIGNLKSLEELYLNDNPLQVLPFELALCTKLALMSVEDCPLSQIPTLAVEGGPSTIMQYLRVKGPYRTMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>F6YVU0</accession>
    <name>F6YVU0_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">Glycylpeptide N-tetradecanoyltransferase</fullName>
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        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">wild type</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Adds a myristoyl group to the N-terminal glycine residue of certain cellular proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>N-terminal glycyl-[protein] + tetradecanoyl-CoA = N-tetradecanoylglycyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15521"/>
            <dbReference type="Rhea" id="RHEA-COMP:12666"/>
            <dbReference type="Rhea" id="RHEA-COMP:12667"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57385"/>
            <dbReference type="ChEBI" id="CHEBI:64723"/>
            <dbReference type="ChEBI" id="CHEBI:133050"/>
            <dbReference type="EC" id="2.3.1.97"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the NMT family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.97" evidence="2 6"/>
    <dbReference type="EMBL" id="EAAA01000845">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6YVU0"/>
    <dbReference type="FunCoup" id="F6YVU0">
        <property type="interactions" value="756"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000012836"/>
    <dbReference type="Ensembl" id="ENSCINT00000012836.3">
        <property type="protein sequence ID" value="ENSCINP00000012836.3"/>
        <property type="gene ID" value="ENSCING00000006217.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000017837"/>
    <dbReference type="HOGENOM" id="CLU_022882_0_1_1"/>
    <dbReference type="InParanoid" id="F6YVU0"/>
    <dbReference type="OMA" id="LHIENEQ"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004379">
        <property type="term" value="F:glycylpeptide N-tetradecanoyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072657">
        <property type="term" value="P:protein localization to membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.170:FF:000001">
        <property type="entry name" value="Glycylpeptide N-tetradecanoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000903">
        <property type="entry name" value="NMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022677">
        <property type="entry name" value="NMT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022678">
        <property type="entry name" value="NMT_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022676">
        <property type="entry name" value="NMT_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11377:SF5">
        <property type="entry name" value="GLYCYLPEPTIDE N-TETRADECANOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11377">
        <property type="entry name" value="N-MYRISTOYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01233">
        <property type="entry name" value="NMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02799">
        <property type="entry name" value="NMT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015892">
        <property type="entry name" value="N-myristl_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00975">
        <property type="entry name" value="NMT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00976">
        <property type="entry name" value="NMT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycylpeptide N-tetradecanoyltransferase N-terminal" evidence="8">
        <location>
            <begin position="16"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="Glycylpeptide N-tetradecanoyltransferase C-terminal" evidence="9">
        <location>
            <begin position="183"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000012836.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="371" mass="43457" checksum="44C4036F8AA4A03F" modified="2012-04-18" version="2">TFTDEDIHEVGVIEADKDNVREEPYTLPPDFEWDTVNLENTKELEEVYKLLNENYVEDDDNMFRFDYSAGFLKWSLRPPNWVKDWHCGVRVRKSKKLVGFISAIPALVKVYDKRQRMVEINFLCVHKKLRTKRVAPVLIREITRRVNQRGVFQAVYTAGIVLPRPVAKCRYYHRSLNPKKLVEVKFSHLGRNQTMQRLVKLMRLPDEPQIQGIRPLKKADVPQCHQLLSNYLSKFDLSPVFTEDEFAHFFLPRKDIVESYVVVDAQGTVTDMASFFILSSSVMHHPDHNMLYAAYSFYNVANKHTEKELMKDMLIFAKKLGFDVFNALDLMDNHVFLKDLKFGMGDGNLHYYLYNYKCPDIPEHKVGLVLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>F6YZ62</accession>
    <accession>A0A1W2VZP5</accession>
    <name>F6YZ62_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Alcohol dehydrogenase-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100184065</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002119381.1">
        <property type="nucleotide sequence ID" value="XM_002119345.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6YZ62"/>
    <dbReference type="FunCoup" id="F6YZ62">
        <property type="interactions" value="25"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000000276"/>
    <dbReference type="Ensembl" id="ENSCINT00000000276.3">
        <property type="protein sequence ID" value="ENSCINP00000000276.3"/>
        <property type="gene ID" value="ENSCING00000000163.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100184065"/>
    <dbReference type="KEGG" id="cin:100184065"/>
    <dbReference type="GeneTree" id="ENSGT00550000075207"/>
    <dbReference type="HOGENOM" id="CLU_026673_11_2_1"/>
    <dbReference type="InParanoid" id="F6YZ62"/>
    <dbReference type="OMA" id="YDDCPAI"/>
    <dbReference type="OrthoDB" id="1879366at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008106">
        <property type="term" value="F:alcohol dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006066">
        <property type="term" value="P:alcohol metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd08240">
        <property type="entry name" value="6_hydroxyhexanoate_dh_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000691">
        <property type="entry name" value="NAD-dependent alcohol dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050129">
        <property type="entry name" value="Zn_alcohol_dh"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43401:SF4">
        <property type="entry name" value="D-ARABINOSE 1-DEHYDROGENASE (NADP(+))"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43401">
        <property type="entry name" value="L-THREONINE 3-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="5">
        <location>
            <begin position="10"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000000276.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="370" mass="40129" checksum="4D7E26BE15C8727C" modified="2012-04-18" version="2">MRKAQIVAPQTPLELCEDAIPTPPPKGAIIKTLYSGICHSDINQWFNEINQGDKKTHFTDNPKYKFPIVPGHEIAGVVHSLGSEVDPDAEDLKVGDEVVAHPWISCGSCRFCYENENSLCDQRHNGLGVGTPGGYATYVWVREKKYLVKVPPSIPLEVACMLPCSGLTTYNAVTTLQPAIERMNKLKGSTSVLLVGAGGLGLWCLQLAKKILPKTTRIVMADINEKNLKVALERGADGTVLWDRNLSEAEAIEITKKAGTDGGFDGVIDMVNSKITAERGFKSTHRGSKMVLVGLYGGGATFALPGFVHGLRTVQGVLVGRLAQLRELVALVAEEKLVPPPLTFYSLDDAFNAMCLVRDGKVQGRCVIKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>F6ZA54</accession>
    <name>F6ZA54_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">wild type</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002267">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6ZA54"/>
    <dbReference type="STRING" id="7719.ENSCINP00000026710"/>
    <dbReference type="Ensembl" id="ENSCINT00000026956.2">
        <property type="protein sequence ID" value="ENSCINP00000026710.2"/>
        <property type="gene ID" value="ENSCING00000014875.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157017"/>
    <dbReference type="HOGENOM" id="CLU_009579_8_0_1"/>
    <dbReference type="InParanoid" id="F6ZA54"/>
    <dbReference type="OMA" id="PEYARIM"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042923">
        <property type="term" value="F:neuropeptide binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007218">
        <property type="term" value="P:neuropeptide signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050119">
        <property type="entry name" value="CCR1-9-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10489">
        <property type="entry name" value="CELL ADHESION MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10489:SF932">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 1 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="32"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="68"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="95"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="148"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="206"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="247"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="291"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="10">
        <location>
            <begin position="48"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000026710.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="313" mass="35462" checksum="26AA1EFFD8986D9E" modified="2011-07-27" version="1">MNSNGIVNTTSTPQISTTTEGYSTLIPEYARIMIIVVYMFVFVFGTIGNACVIVVMMIVPKFKTPTDIFILNLSFADLLFSSSLIFWAVEQIYEMVWFLGVFMCKALSAVTLINLHASVLFLAAMAVNRCKTVRRKRQSSSYGDIIKAKVSCVIIWLVAAIISSLAWFRKTEIVPSPAGNVTRCLWRMPDTGDRYVWERIYFGSRVLMGFTLPLIVIIISYVLVVMRLRHTRQQVTVKSSMQMRVTITVFAVIAAFVICWLPNHVTSIIHALTLGENEEKRKDLHTYELNLISNCLVAFNSCINPILYAWLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>F6ZDG8</accession>
    <accession>A0A1W2WF37</accession>
    <name>F6ZDG8_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ciliary microtubule inner protein 2B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100182355</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Microtubule inner protein (MIP) part of the dynein-decorated doublet microtubules (DMTs) in cilia axoneme, which is required for motile cilia beating.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the CIMIP2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002130074.1">
        <property type="nucleotide sequence ID" value="XM_002130038.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6ZDG8"/>
    <dbReference type="STRING" id="7719.ENSCINP00000021297"/>
    <dbReference type="Ensembl" id="ENSCINT00000021297.3">
        <property type="protein sequence ID" value="ENSCINP00000021297.3"/>
        <property type="gene ID" value="ENSCING00000012328.2"/>
    </dbReference>
    <dbReference type="GeneID" id="100182355"/>
    <dbReference type="KEGG" id="cin:100182355"/>
    <dbReference type="GeneTree" id="ENSGT00940000166013"/>
    <dbReference type="HOGENOM" id="CLU_065650_1_0_1"/>
    <dbReference type="InParanoid" id="F6ZDG8"/>
    <dbReference type="OMA" id="TRYADIC"/>
    <dbReference type="OrthoDB" id="2019884at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015630">
        <property type="term" value="C:microtubule cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018902">
        <property type="entry name" value="CMI2A-C-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22146">
        <property type="entry name" value="CAT EYE SYNDROME CRITICAL REGION PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22146:SF8">
        <property type="entry name" value="PROTEIN FAM166B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10629">
        <property type="entry name" value="CMI2B-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0966">Cell projection</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ciliary microtubule inner protein 2A-C-like" evidence="8">
        <location>
            <begin position="14"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00041163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF10629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000021297.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="287" mass="32645" checksum="83C3D3C411E4AA10" modified="2012-04-18" version="2">MAAVGPKRTTFCNEPYYPPGYCGYSPQFHFQYGDTYGKTTSRLLTDPNVAKSGRLVLADLFPAENSGNDVTDERNEWKRLRKQSWGDKKLNDKMVPGYTGYIPKKEDHFGTRYADICRKATKDFSNEMSAAKDKRRALQPRMPMTPKLAEPKPYVSLTQPQHSVSPYFMNINDGAKTFMSGYTGFIPRSRSRFALVYPKLTRESLKEFTRNQQELKLLANREVSINQPGPSDLPVNPLQPIYINGGLLPHYTGYLPGHKFRVGMTFGTSSRSFHATNQPRYNKSLFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>F6ZFT6</accession>
    <name>F6ZFT6_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Charged multivesicular body protein 7</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">wild type</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF7 family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002610">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="STRING" id="7719.ENSCINP00000024521"/>
    <dbReference type="Ensembl" id="ENSCINT00000024767.2">
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        <property type="gene ID" value="ENSCING00000013325.2"/>
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        <property type="component" value="Chromosome 8"/>
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        <property type="term" value="C:multivesicular body"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0032511">
        <property type="term" value="P:late endosome to vacuole transport via multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006900">
        <property type="term" value="P:vesicle budding from membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN"/>
        <property type="match status" value="1"/>
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    </dbReference>
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    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="41"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000024521.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="454" mass="51956" checksum="6BD9EA238AB58EE6" modified="2012-04-18" version="2">MVDIIEEIKAGNLFTLISNYTNGIYSELVEANKSGDWQVQVLLVECVICNCLVFLICVAWKIYSRSITEHFSKENNNCWNDEQRMNFLFSAFPPDPSHNPAHWKSKLDFWRTLIMDLCVQNNRMKVTLDTVSVWVARDDRKPMGLHVVWEDLKQSKEFVSASEYAQDLSRNSTWLGWGLNVLVRKPTTWITGKVFSPLKGKGDFGSQQYICMKSLHKMCHLVLERVHEEKQKEDHPNKTYIYSYKQLQALTSDIVNGTDCLDLVILTLENKKSASVYMPEPTAAAGTSSPSEVDLGILELEESEKVLEKEITFLYTEQTKLVNLCRESIRNKHRVLAKNYLRKKRRLTVVLDKKDASLSNVQQLLERIHQCKTDMLVVETYKAGIEMYKKLKDEGGLNIDAVEDTMNDLGDMIAEQDDISSIMATPVSESEFSNDELEQELNDLLSEDISTPAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>F6ZG55</accession>
    <name>F6ZG55_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">wild type</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01002611">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6ZG55"/>
    <dbReference type="Ensembl" id="ENSCINT00000024763.2">
        <property type="protein sequence ID" value="ENSCINP00000024517.2"/>
        <property type="gene ID" value="ENSCING00000008073.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163854"/>
    <dbReference type="HOGENOM" id="CLU_864749_0_0_1"/>
    <dbReference type="InParanoid" id="F6ZG55"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050527">
        <property type="entry name" value="Snail/Krueppel_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24388:SF90">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24388">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="11"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="186"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000024517.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="322" mass="37622" checksum="8B2C3C87FD8E748D" modified="2012-04-18" version="2">MDNSELTRGPFTCPNCNKEFQQFSVFNAHSQEKCVPADSQPFCIIFNPTSNKNARPRKKLHLTVEEYFYGKCNGKKHKQVANFNPSKKYKCYSCAKVLKNNLRFMSHVRHKIENHWKQNDNINDFNICSHCYRHYDTPFQLQCHVETVHSSVAASTTCCICELNFQDEETFLFHMKNRHGCQEMPYVCKICSHRSSLYSDLMQHFRECHENSNSLMCPFCLCIHNTGNSHLNHVIQHLKKSYRCSRCRLQFSSSVAQCQHLTIDHMKTMRSKLRYPVPPNINDYTVYVKLRKKASLPLKSFFFGSPTAREVLATSFPNFVDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>F6ZNK2</accession>
    <name>F6ZNK2_CIOIN</name>
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        <recommendedName>
            <fullName evidence="4">Kinase</fullName>
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        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100182374</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the inositol phosphokinase (IPK) family.</text>
    </comment>
    <dbReference type="EC" id="2.7.-.-" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="F6ZNK2"/>
    <dbReference type="FunCoup" id="F6ZNK2">
        <property type="interactions" value="34"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSCINT00000006649.3">
        <property type="protein sequence ID" value="ENSCINP00000006649.3"/>
        <property type="gene ID" value="ENSCING00000003245.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000167316"/>
    <dbReference type="HOGENOM" id="CLU_014862_0_0_1"/>
    <dbReference type="InParanoid" id="F6ZNK2"/>
    <dbReference type="OMA" id="VYQADTG"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000828">
        <property type="term" value="F:inositol hexakisphosphate kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032958">
        <property type="term" value="P:inositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.160">
        <property type="entry name" value="Inositol polyphosphate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005522">
        <property type="entry name" value="IPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038286">
        <property type="entry name" value="IPK_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12400">
        <property type="entry name" value="INOSITOL POLYPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12400:SF21">
        <property type="entry name" value="KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03770">
        <property type="entry name" value="IPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000006649.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="431" mass="49051" checksum="EC003B3B0087938F" modified="2012-04-18" version="2">MTDQPPKLHISDEHLCENGVTLEPFLHQVAGRATMMCLNDTTVCKPLNYREHFFYKSLPPDILQFTPKFKGVVNVMIEETNDGSVALTAFPYKPETVEDDIESSSTESPVSDSSEESLQKLGGAYIANVNEQMSNMFSSTCRLDNPRKIWSDIVEIQLHSYSVDGQYHRHNSDGEDGNMGLCINPWIFKVHKEGLLRHLQSKHKVIQKCILLENVASQFRKPCVLDLKMGTRIHGDFDDEEKRLRHEKKCAATTTKKLGIRLSGMQIFDASTGSFLCRDKYFGRKLDRAGLRGLLRQFFYDGCRLRRDLIRPILSRLQLILESLGRLRSYRFYSSSLLIMYDGETLESTSHIPAAPLSEQNNISPHPTPAEERSHLEIDPGVQFDVRMIDFAKSTHQEMDRSVIHDGPDHGYIFGLTNLIALLQDISKSDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>F7A2U0</accession>
    <name>F7A2U0_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">wild type</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the gamma-glutamyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001732">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F7A2U0"/>
    <dbReference type="STRING" id="7719.ENSCINP00000003836"/>
    <dbReference type="Ensembl" id="ENSCINT00000003836.3">
        <property type="protein sequence ID" value="ENSCINP00000003836.3"/>
        <property type="gene ID" value="ENSCING00000001905.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165663"/>
    <dbReference type="HOGENOM" id="CLU_1690693_0_0_1"/>
    <dbReference type="InParanoid" id="F7A2U0"/>
    <dbReference type="OMA" id="WDNATHG"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036374">
        <property type="term" value="F:glutathione hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006751">
        <property type="term" value="P:glutathione catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.246.130:FF:000002">
        <property type="entry name" value="glutathione hydrolase 1 proenzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043138">
        <property type="entry name" value="GGT_lsub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000101">
        <property type="entry name" value="GGT_peptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043137">
        <property type="entry name" value="GGT_ssub_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11686">
        <property type="entry name" value="GAMMA GLUTAMYL TRANSPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11686:SF9">
        <property type="entry name" value="RE13973P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01019">
        <property type="entry name" value="G_glu_transpept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01210">
        <property type="entry name" value="GGTRANSPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="2">
        <location>
            <position position="96"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="114"/>
            <end position="116"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR600101-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR600101-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000003836.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="156" mass="17477" checksum="0198FD39E0512E16" modified="2012-04-18" version="2">MSLILNILKGYKFSPKSMSKENRVLTMHRIVEAFRFAFAKRSYLGGEPNYPNMTELVKNMTADWYADDLRLKINDDHTWPVDYYGPDWSVPDDSGTAHLSVLAPNGDAVAITSTINLYFGSKVRGKYTGIIFNNEMDDFSSPNITNAFGVPPSPAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>F7ACS2</accession>
    <name>F7ACS2_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">wild type</strain>
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    <reference evidence="16" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
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            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
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            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
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            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
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            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
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        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="GO_Central"/>
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            <position position="33"/>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
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        <name type="synonym">Ascidia intestinalis</name>
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            <taxon>Phlebobranchia</taxon>
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    <reference evidence="14" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">wild type</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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    <reference evidence="13" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="ENSCING00000001743.3"/>
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    <dbReference type="GeneTree" id="ENSGT00940000164120"/>
    <dbReference type="HOGENOM" id="CLU_018808_11_1_1"/>
    <dbReference type="InParanoid" id="F7AMK6"/>
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        <property type="component" value="Chromosome 7"/>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:symporter activity"/>
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        <property type="term" value="P:sodium ion transport"/>
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    <dbReference type="Gene3D" id="1.20.1730.10">
        <property type="entry name" value="Sodium/glucose cotransporter"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Na/Glc_symporter_sf"/>
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    <dbReference type="InterPro" id="IPR001734">
        <property type="entry name" value="Na/solute_symporter"/>
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    <dbReference type="InterPro" id="IPR051163">
        <property type="entry name" value="Sodium:Solute_Symporter_SSF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00813">
        <property type="entry name" value="sss"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42985">
        <property type="entry name" value="SODIUM-COUPLED MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42985:SF45">
        <property type="entry name" value="SODIUM_IODIDE COTRANSPORTER-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00474">
        <property type="entry name" value="SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50283">
        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0915">Sodium</keyword>
    <keyword evidence="10" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="15"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="55"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="85"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="129"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="160"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="191"/>
            <end position="209"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="242"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="281"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="348"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="384"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="411"/>
            <end position="435"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="442"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="526"/>
            <end position="547"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU362091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000003526.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="549" mass="59256" checksum="CB69377C22E8F0B8" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>F7AWP0</accession>
    <name>F7AWP0_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ornithine decarboxylase</fullName>
            <ecNumber evidence="7">4.1.1.17</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">wild type</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the first and rate-limiting step of polyamine biosynthesis that converts ornithine into putrescine, which is the precursor for the polyamines, spermidine and spermine. Polyamines are essential for cell proliferation and are implicated in cellular processes, ranging from DNA replication to apoptosis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-ornithine + H(+) = putrescine + CO2</text>
            <dbReference type="Rhea" id="RHEA:22964"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:326268"/>
            <dbReference type="EC" id="4.1.1.17"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="6">Amine and polyamine biosynthesis; putrescine biosynthesis via L-ornithine pathway; putrescine from L-ornithine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homodimer. Only the dimer is catalytically active, as the active sites are constructed of residues from both monomers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Orn/Lys/Arg decarboxylase class-II family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.17" evidence="7"/>
    <dbReference type="EMBL" id="EAAA01001491">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F7AWP0"/>
    <dbReference type="FunCoup" id="F7AWP0">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000021832"/>
    <dbReference type="Ensembl" id="ENSCINT00000022078.2">
        <property type="protein sequence ID" value="ENSCINP00000021832.2"/>
        <property type="gene ID" value="ENSCING00000011423.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182995"/>
    <dbReference type="HOGENOM" id="CLU_026444_1_1_1"/>
    <dbReference type="InParanoid" id="F7AWP0"/>
    <dbReference type="OMA" id="WINDGIH"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004586">
        <property type="term" value="F:ornithine decarboxylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033387">
        <property type="term" value="P:putrescine biosynthetic process from arginine, via ornithine"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00622">
        <property type="entry name" value="PLPDE_III_ODC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.10:FF:000005">
        <property type="entry name" value="Ornithine decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.37.10:FF:000038">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.10">
        <property type="entry name" value="Alanine racemase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.37.10">
        <property type="entry name" value="Lyase, Ornithine Decarboxylase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009006">
        <property type="entry name" value="Ala_racemase/Decarboxylase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022657">
        <property type="entry name" value="De-COase2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022644">
        <property type="entry name" value="De-COase2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022653">
        <property type="entry name" value="De-COase2_pyr-phos_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000183">
        <property type="entry name" value="Orn/DAP/Arg_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002433">
        <property type="entry name" value="Orn_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029066">
        <property type="entry name" value="PLP-binding_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11482">
        <property type="entry name" value="ARGININE/DIAMINOPIMELATE/ORNITHINE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11482:SF6">
        <property type="entry name" value="ORNITHINE DECARBOXYLASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02784">
        <property type="entry name" value="Orn_Arg_deC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01179">
        <property type="entry name" value="ODADCRBXLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01182">
        <property type="entry name" value="ORNDCRBXLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50621">
        <property type="entry name" value="Alanine racemase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51419">
        <property type="entry name" value="PLP-binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00878">
        <property type="entry name" value="ODR_DC_2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00879">
        <property type="entry name" value="ODR_DC_2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0620">Polyamine biosynthesis</keyword>
    <keyword evidence="3 11" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Orn/DAP/Arg decarboxylase 2 N-terminal" evidence="12">
        <location>
            <begin position="11"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="11">
        <location>
            <position position="324"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="11">
        <location>
            <position position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00046672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR600183-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000021832.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="378" mass="42582" checksum="F4BE49A827778F46" modified="2012-04-18" version="2">DGSFYIGDLNRVFLRHQQWKEVLPRVELFYAIKCCPLMPVIRLLAALGTGFDCASQNEIESVISIGVDPAKIIYALPCKPASQLQHARTRKVSLMTFDNEDELHKIQKYFPDARLVLRLLPDETGATFKMGSKFGCTLEEGKKLLVHAKKLDLNVIGLSFFVGDDCKSSIGMRLNLENSRKMFDFASSIGINMDLIDIGGGFPGNDKDQKLFLQIANDTRDTLDKLFPSECGVRIIAEPGRYFVESSFSIATSIVGKKQSEGIWCRIVFNAETTDIIFYYTNESIYLAFWKTFLKAGKYTPIVLKTKRFSDKAVHDTIIYGTTCDGTDIIAQNVALPELDLEDWLIWDDMGAYTVLCKFGFNGVMTTKAFYFVQEGAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>F7BEK9</accession>
    <accession>A0A1W2WDP0</accession>
    <name>F7BEK9_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized oxidoreductase SERP2049</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC100179397</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002075">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002129412.1">
        <property type="nucleotide sequence ID" value="XM_002129376.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F7BEK9"/>
    <dbReference type="Ensembl" id="ENSCINT00000023258.2">
        <property type="protein sequence ID" value="ENSCINP00000023012.2"/>
        <property type="gene ID" value="ENSCING00000012291.2"/>
    </dbReference>
    <dbReference type="GeneID" id="100179397"/>
    <dbReference type="KEGG" id="cin:100179397"/>
    <dbReference type="GeneTree" id="ENSGT00940000164675"/>
    <dbReference type="HOGENOM" id="CLU_010194_1_3_1"/>
    <dbReference type="InParanoid" id="F7BEK9"/>
    <dbReference type="OMA" id="GMTYTPM"/>
    <dbReference type="OrthoDB" id="37659at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000084">
        <property type="entry name" value="Short-chain dehydrogenase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43975:SF2">
        <property type="entry name" value="EG:BACR7A4.14 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43975">
        <property type="entry name" value="ZGC:101858"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000023012.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="256" mass="27514" checksum="64EE6751F8D31658" modified="2011-07-27" version="1">MNELKDKVVVITGASSGIGEEIAYTFAKQGAALGVCGRNKEKLGKVAEQCKAHGAGKVIEIIGDLMKLNDIDQMVEEVVSKLGDVDVLINNCGAIVNGGIETPNVEDFDKIFHVNVRAPFYLTQKCVPHLKKTKGCVVNISTLLTKICETNNLLYSMAKCSIDHFTKSTALELAKYDIRVNSVNPAVVLTPILQQMFSPEKLSEVLGEVGPMHPLGSKCLSTQEIADTVIFLSTSGARCITGTCLLADRGRGVFGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>F7BNZ7</accession>
    <name>F7BNZ7_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">MyTH4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">wild type</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002144">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="EAAA01002145">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="EAAA01002146">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F7BNZ7"/>
    <dbReference type="STRING" id="7719.ENSCINP00000022929"/>
    <dbReference type="Ensembl" id="ENSCINT00000023175.2">
        <property type="protein sequence ID" value="ENSCINP00000022929.2"/>
        <property type="gene ID" value="ENSCING00000012224.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00930000151032"/>
    <dbReference type="HOGENOM" id="CLU_021039_2_0_1"/>
    <dbReference type="InParanoid" id="F7BNZ7"/>
    <dbReference type="OMA" id="TDCVYEL"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.530">
        <property type="entry name" value="MyTH4 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000299">
        <property type="entry name" value="FERM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000857">
        <property type="entry name" value="MyTH4_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038185">
        <property type="entry name" value="MyTH4_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051567">
        <property type="entry name" value="Unconventional_Myosin_ATPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22692">
        <property type="entry name" value="MYOSIN VII, XV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22692:SF26">
        <property type="entry name" value="SH3 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00373">
        <property type="entry name" value="FERM_M"/>
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</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>H2XJL0</accession>
    <name>H2XJL0_CIOIN</name>
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        <submittedName>
            <fullName evidence="7">Sialin-like</fullName>
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        <name evidence="7" type="primary">LOC100175186</name>
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        <name type="scientific">Ciona intestinalis</name>
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            <taxon>Eukaryota</taxon>
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    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">wild type</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_026836671.1"/>
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    <dbReference type="Ensembl" id="ENSCINT00000033408.1">
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        <property type="gene ID" value="ENSCING00000024681.1"/>
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        <property type="component" value="Chromosome 11"/>
    </dbReference>
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        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="sialin-like isoform X1"/>
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    </dbReference>
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        <property type="entry name" value="MFS general substrate transporter like domains"/>
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    </dbReference>
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        <property type="entry name" value="MFS"/>
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        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050382">
        <property type="entry name" value="MFS_Na/Anion_cotransporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
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        <property type="entry name" value="FI19708P1-RELATED"/>
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    </dbReference>
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        <property type="entry name" value="SOLUTE CARRIER FAMILY 17"/>
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        <property type="entry name" value="MFS general substrate transporter"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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            <begin position="30"/>
            <end position="50"/>
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            <begin position="92"/>
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            <begin position="187"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000029842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
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</entry>
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    <accession>H2XJN9</accession>
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        <submittedName>
            <fullName evidence="3">Epimerase family protein SDR39U1</fullName>
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    </protein>
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        <name evidence="3" type="primary">LOC100181474</name>
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        <lineage>
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        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="RefSeq" id="XP_002127876.1">
        <property type="nucleotide sequence ID" value="XM_002127840.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XJN9"/>
    <dbReference type="FunCoup" id="H2XJN9">
        <property type="interactions" value="107"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000029871"/>
    <dbReference type="Ensembl" id="ENSCINT00000035182.1">
        <property type="protein sequence ID" value="ENSCINP00000029871.1"/>
        <property type="gene ID" value="ENSCING00000023803.1"/>
    </dbReference>
    <dbReference type="GeneID" id="100181474"/>
    <dbReference type="KEGG" id="cin:100181474"/>
    <dbReference type="GeneTree" id="ENSGT00390000000337"/>
    <dbReference type="HOGENOM" id="CLU_047373_0_1_1"/>
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    <dbReference type="OMA" id="YLPWIHI"/>
    <dbReference type="OrthoDB" id="276721at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 2"/>
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    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
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        <property type="entry name" value="DUF1731"/>
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        <property type="entry name" value="Epimerase_deHydtase"/>
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        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="SDR39U1"/>
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        <property type="entry name" value="yfcH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11092:SF0">
        <property type="entry name" value="EPIMERASE FAMILY PROTEIN SDR39U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11092">
        <property type="entry name" value="SUGAR NUCLEOTIDE EPIMERASE RELATED"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF08338">
        <property type="entry name" value="DUF1731"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="1">
        <location>
            <begin position="6"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1731" evidence="2">
        <location>
            <begin position="247"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000029871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="297" mass="32461" checksum="5FC495E748531023" modified="2012-04-18" version="1">MTKLAVVGGGNGFIGRALAKQLQKSGYKALSVSRKPGENTLTWKSIETEGLPKNTEVVINLTGEPVLNVFKRWTPNFKQEVWDSRIKTTKTLRTAVLAADTPPKVWATVSGVGFYPPSTSRIYDELSVPESTDFWSELTHAWENSGRIKNENTRHFVVRSGVVLGKNGGAMSEMLPPFKLGLGGPMGDGKQWFPWIHIDDIAGIFMHGVKNTSVRGVLNGVAPEAHTNGEFSKALGSVLSRPALLPLPSFVVKALYGSERSVMLLQGQNVYPKLTLDSGYVYSYPKLDAALKEVTSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>H2XKA1</accession>
    <name>H2XKA1_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Small nuclear ribonucleoprotein E</fullName>
            <shortName evidence="12">snRNP-E</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Sm protein E</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">wild type</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Plays a role in pre-mRNA splicing as a core component of the spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Component of both the pre-catalytic spliceosome B complex and activated spliceosome C complexes. As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs. As part of the U7 snRNP it is involved in histone 3'-end processing.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the snRNP Sm proteins family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01003016">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XKA1"/>
    <dbReference type="FunCoup" id="H2XKA1">
        <property type="interactions" value="803"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000030083"/>
    <dbReference type="Ensembl" id="ENSCINT00000034525.1">
        <property type="protein sequence ID" value="ENSCINP00000030083.1"/>
        <property type="gene ID" value="ENSCING00000023749.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000012818"/>
    <dbReference type="HOGENOM" id="CLU_125186_1_0_1"/>
    <dbReference type="InParanoid" id="H2XKA1"/>
    <dbReference type="OMA" id="VPPINCI"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034715">
        <property type="term" value="C:pICln-Sm protein complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005687">
        <property type="term" value="C:U4 snRNP"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000387">
        <property type="term" value="P:spliceosomal snRNP assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01718">
        <property type="entry name" value="Sm_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000013">
        <property type="entry name" value="Small nuclear ribonucleoprotein E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027078">
        <property type="entry name" value="snRNP-E"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11193">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 12" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8 12" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="9 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 12" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="7 12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 12" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Sm" evidence="13">
        <location>
            <begin position="14"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00045276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU365053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000030083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="90" mass="10579" checksum="4D872659FFF7897D" modified="2012-04-18" version="1">YKGQKVQKVMVQPINLIFRYLQNRSRVSIWVYEQVNMRIEGHIVGFDEYMNLVLDDAEEVNIKKNVRKTLGRVMLKGDNITVIQQVQELG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>H2XL50</accession>
    <name>H2XL50_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
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                <person name="Wang H.G."/>
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                <person name="Azumi K."/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Chapman J."/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="De Tomaso A."/>
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                <person name="Hastings K.E."/>
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                <person name="Kawashima T."/>
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                <person name="Meinertzhagen I.A."/>
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                <person name="Putnam N."/>
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                <person name="Terry A."/>
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                <person name="Wang H.G."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Matsumoto J."/>
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                <person name="Wiley G.B."/>
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <comment type="function">
        <text evidence="7">May act as a transporter of organic cations based on a proton efflux antiport mechanism. May play a role in the transport of chloroquine and quinidine-related compounds in kidney. Plays a role in the regulation of lipid metabolism.</text>
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        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome 11"/>
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        <property type="term" value="C:apical plasma membrane"/>
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        <property type="term" value="C:nuclear envelope"/>
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        <property type="term" value="F:transmembrane transporter activity"/>
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        <property type="entry name" value="Solute carrier family 22 member 18"/>
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        <property type="entry name" value="MFS general substrate transporter like domains"/>
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        <property type="entry name" value="Tet-R_TetA/multi-R_MdtG-like"/>
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        <property type="entry name" value="SOLUTE CARRIER FAMILY 22 MEMBER 18"/>
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        <property type="entry name" value="SOLUTE CARRIER FAMILY 22 MEMBER 18"/>
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        <property type="entry name" value="MFS_1"/>
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    <feature type="transmembrane region" description="Helical" evidence="8">
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        <location>
            <begin position="1"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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    <sequence length="399" mass="43410" checksum="A6E87FF598430944" modified="2012-04-18" version="1">MVNFDKNRIILLSHLNVTLYALCFWIQIGTLPYLTQKFDVDPITFGNLQTTFAVVQLMGGPLFGRFGDLFGCKYALALAFISAFLSYFLLGVANTVFIIFLSRLPSVFMHAMQGAQMVITDLTEPSERPDKLGKLGLSYAVGMVLGPVIGGWCTTYANEQVAAFVAACGSLLSIVLVVLFIPSNTKGFILKENTEKTLKKSKKLLDFDVFTSLIWKNPSARKLLFVKMLSGLPVGILHGMFSMIALNHFHLSAEANGYVLSYAGFLSIISQGLVIGRLTKRGFNEWSLINGCVAVLCVSNALMSILVNGVIGFCISLIPMVVAGAVFSTVIQAMLTKTVQSEDTGSMLGVSMAVHSLIRSVSPTIGGFLFQYYGFWSFGAIGFLCNGLLLVYLLISNPR</sequence>
</entry>
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            <fullName evidence="1">Uncharacterized protein</fullName>
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        <name type="scientific">Ciona intestinalis</name>
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        <name type="synonym">Ascidia intestinalis</name>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">wild type</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000030860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="41" mass="4740" checksum="E95FC8D087BD40BE" modified="2012-04-18" version="1">CKSVKFKSLTSNLCKPYRFSEMSSFTEAKANRLAKHKQKDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>H2XN88</accession>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
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    <organism evidence="1 2">
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        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
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                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
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                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="H2XN88"/>
    <dbReference type="Ensembl" id="ENSCINT00000033347.1">
        <property type="protein sequence ID" value="ENSCINP00000031121.1"/>
        <property type="gene ID" value="ENSCING00000020471.1"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2440199_0_0_1"/>
    <dbReference type="InParanoid" id="H2XN88"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000031121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="90" mass="9788" checksum="1E61A6EEA84E7E9E" modified="2012-04-18" version="1">MSSYSGISSFFRGRTTALKGNGKKPNPVIPSSIGGRSWLSGSGRTLNLLIMLARKRKISILANFSPRHDLHPIPNGITFLEGMNFPSLSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>H2XP98</accession>
    <name>H2XP98_CIOIN</name>
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        <recommendedName>
            <fullName evidence="4">ZP domain-containing protein</fullName>
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    <organism evidence="5 6">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">wild type</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01001511">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="EAAA01001512">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XP98"/>
    <dbReference type="Ensembl" id="ENSCINT00000031097.1">
        <property type="protein sequence ID" value="ENSCINP00000031481.1"/>
        <property type="gene ID" value="ENSCING00000023303.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00660000097179"/>
    <dbReference type="HOGENOM" id="CLU_1207143_0_0_1"/>
    <dbReference type="InParanoid" id="H2XP98"/>
    <dbReference type="OMA" id="ECKSHEI"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 2"/>
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    <dbReference type="Gene3D" id="2.60.40.3210">
        <property type="entry name" value="Zona pellucida, ZP-N domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055356">
        <property type="entry name" value="ZP-N"/>
    </dbReference>
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        <property type="entry name" value="ZP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14002:SF43">
        <property type="entry name" value="DELTA-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14002">
        <property type="entry name" value="ENDOGLIN/TGF-BETA RECEPTOR TYPE III"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZP-N"/>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="20"/>
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    <feature type="chain" id="PRO_5003578068" description="ZP domain-containing protein" evidence="3">
        <location>
            <begin position="21"/>
            <end position="230"/>
        </location>
    </feature>
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        <location>
            <begin position="64"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000031481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="230" mass="25364" checksum="263F590770569892" modified="2012-04-18" version="1">MRVKLYLLLISACFWCGVYSQSGSSQPCPTTCPTYSKCSTNGTCECESGFLSMSGKCVADILLECKSHEITIQIQEDVFKTAKFNVDTAVVGGNTSCTATVQGDNIIFKFANDDCSTTVLRNDTHTSYSNFIQTSATIEDKITYGSRGRIPFTCVFPHTSNISVAYNATLSMNPLPDVAPVTGKFDIDFQLVKNDYNTVQGRRIFLTNETLYFKASLNNADTDQLLVFDR</sequence>
</entry>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    <dbReference type="STRING" id="7719.ENSCINP00000031802"/>
    <dbReference type="Ensembl" id="ENSCINT00000032529.1">
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        <property type="gene ID" value="ENSCING00000018732.1"/>
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    <dbReference type="HOGENOM" id="CLU_117692_0_0_1"/>
    <dbReference type="InParanoid" id="H2XQ68"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Leucine-rich Repeat Variant"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Formin GTPase-binding" evidence="2">
        <location>
            <begin position="53"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000031802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="190" mass="22200" checksum="E2659EB3E497C5A5" modified="2012-04-18" version="1">MDKFDTKRKSKFLDRFASSANTKPKKGKDKYAYKTPSISEDHPTDNMIPDVVATKLSEREVLTLFEKMLDDMNLAEERKEPLRSKDLEIKRSMVVQWLNKTTTVKGEQAMTPQQFIYNMQSLVRKEESYRGESSRELLQVLESLRVSLNSNPLSWVQNFGREGLDILLRILNDCYDNNYTSEIHNKIKLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>H2XQG3</accession>
    <name>H2XQG3_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the KCMF1 family.</text>
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    <dbReference type="EC" id="2.3.2.27" evidence="3"/>
    <dbReference type="EMBL" id="EAAA01002740">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="FunCoup" id="H2XTC5">
        <property type="interactions" value="6"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000032909"/>
    <dbReference type="Ensembl" id="ENSCINT00000030355.1">
        <property type="protein sequence ID" value="ENSCINP00000032909.1"/>
        <property type="gene ID" value="ENSCING00000005635.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00510000047171"/>
    <dbReference type="HOGENOM" id="CLU_032080_1_1_1"/>
    <dbReference type="InParanoid" id="H2XTC5"/>
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        <property type="component" value="Chromosome 8"/>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="project" value="GOC"/>
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        <property type="term" value="F:ubiquitin protein ligase activity"/>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:synaptic signaling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="entry name" value="ZZ_PCMF_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008598">
        <property type="entry name" value="Di19_Zn-bd"/>
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    <dbReference type="InterPro" id="IPR050774">
        <property type="entry name" value="KCMF1/Dystrophin"/>
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        <property type="entry name" value="Znf_ZZ"/>
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        <property type="entry name" value="Znf_ZZ_sf"/>
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    <dbReference type="PANTHER" id="PTHR12268">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE KCMF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12268:SF13">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE KCMF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05605">
        <property type="entry name" value="zf-Di19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00569">
        <property type="entry name" value="ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00291">
        <property type="entry name" value="ZnF_ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01357">
        <property type="entry name" value="ZF_ZZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50135">
        <property type="entry name" value="ZF_ZZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ZZ-type" evidence="10">
        <location>
            <begin position="4"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="205"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
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            <dbReference type="PROSITE-ProRule" id="PRU00228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000032909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="347" mass="38428" checksum="29C168EAE7CDBB99" modified="2012-04-18" version="1">MSRHEGVSCDNCLKSNFEGKRFKCLICYDYDLCESCHVFEINTSRHSSTHPMQCILTRNEFELYYGGENISPESPPSFVCPFCAKLGFTEALLHEHVNKEHADAHSEVVCPICAALPSGDPNLVTDELATHLSMEHRAPRDAEDQAGGRHIRRMFYPGRGMRSRNHRNMHFSSGPATSAIPTPAVANNNVASSVISAGRDSMDPIAELLSQLSGVRRSAQQLQQTVTTSSQLQELQSQLQMERQRAAELRQQLETKSASITQLLNTSSCVGVLIQGSGLTNSNNHANSQENSTADNKQFVVTFLDELNLSSKEKEQQSNEDGQRSLFVQELLLSIFSEGADSHLEQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>H2XTE5</accession>
    <name>H2XTE5_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="H2XTE5"/>
    <dbReference type="Ensembl" id="ENSCINT00000035429.1">
        <property type="protein sequence ID" value="ENSCINP00000032929.1"/>
        <property type="gene ID" value="ENSCING00000023902.1"/>
    </dbReference>
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    <dbReference type="InParanoid" id="H2XTE5"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
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        <property type="entry name" value="TSP_1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="TSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003578156" evidence="1">
        <location>
            <begin position="22"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000032929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="185" mass="20797" checksum="F5C6670A90FAA006" modified="2012-04-18" version="1">MKVGLWFLTLAADFIIRESQAVPSSTEEGSWSKCSVTCGEGYQTKQQTLNKFTFGEIKNQTIRRSCNLQVCPSYLGWPRIHFYGTFVANCPTTNNFRCFFNNDKFCPVCNTFRLTTATEVKFHQAGPTVHRYGFQRGSADPMGNGHFYFDGAKVTSACWKPGQACLTKDPIVGRKVTDKNTGRMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>H2XTF2</accession>
    <name>H2XTF2_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptidase M12A domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="H2XTF2"/>
    <dbReference type="MEROPS" id="M12.007"/>
    <dbReference type="Ensembl" id="ENSCINT00000036573.1">
        <property type="protein sequence ID" value="ENSCINP00000032936.1"/>
        <property type="gene ID" value="ENSCING00000019778.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000171675"/>
    <dbReference type="HOGENOM" id="CLU_1656076_0_0_1"/>
    <dbReference type="InParanoid" id="H2XTF2"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001506">
        <property type="entry name" value="Peptidase_M12A"/>
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    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        <source>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="1">Belongs to the NDRG family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    </evidence>
    <sequence length="368" mass="40909" checksum="1A4E09CC1FA47763" modified="2012-04-18" version="1">CKMDLQEVNITPDKPLLQDKHSNESEDNVVECTYTAAGNHYSTKLHVVVQGNRSLGHSIVTFHDVGLNSVSNFGALMNSEAMEPVAHKYCVYHINAPGQEEHARTLPSGHPYPTMENLSDMVPKIFQEFGIKSAICLGSGAGANVFLRFAFKNPSMVEGLIAVNPTISTVGNLSWIGEKITNWTTPFSDQIMNYYFTKSEVELQNHELLDTHRIHFKKFMNEENVINFMKSYERRSDINITRSPDPQQVDKTTLKCQTLILVGDLSPFVDEAVEVNSRLNVKKTTFLKMADAGGMILEEQIFNVAEAITYFLQGLGHIPGVMMTRLARSRTISNSSTGSADGDHKRIRTLSGGSANMDFSPKTEVVIC</sequence>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
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                <person name="Harafuji N."/>
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                <person name="Ho I."/>
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                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
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                <person name="Meinertzhagen I.A."/>
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                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
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                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
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                <person name="Nomoto H."/>
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                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
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            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
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    <feature type="chain" id="PRO_5003577001" evidence="1">
        <location>
            <begin position="22"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000033261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="56" mass="6233" checksum="BC07F535CBC9D82F" modified="2012-04-18" version="1">MSVHCACMAAIRMLFPRSTFALGSDVICDDVHEDDVLCSHDVTMFVYLEVLGRALQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>H2XUE6</accession>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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    <reference evidence="9" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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            <strain evidence="9">wild type</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="7" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
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                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
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</entry>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="H2XUZ1"/>
    <dbReference type="Ensembl" id="ENSCINT00000033029.1">
        <property type="protein sequence ID" value="ENSCINP00000033475.1"/>
        <property type="gene ID" value="ENSCING00000018702.1"/>
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    <dbReference type="HOGENOM" id="CLU_3207344_0_0_1"/>
    <dbReference type="InParanoid" id="H2XUZ1"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000033475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="45" mass="5320" checksum="683FFC4806381C39" modified="2012-04-18" version="1">MAILRNICKNVFFIDELGIYKDIFTILIGQEPKLCYFIAKIRINQ</sequence>
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    <accession>H2XV25</accession>
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    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
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    <organism evidence="1 2">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ciona</taxon>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">wild type</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01000659">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="H2XV25"/>
    <dbReference type="STRING" id="7719.ENSCINP00000033509"/>
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        <property type="protein sequence ID" value="ENSCINP00000033509.1"/>
        <property type="gene ID" value="ENSCING00000023805.1"/>
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    <dbReference type="OMA" id="CDASFGW"/>
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        <property type="component" value="Chromosome 11"/>
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    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="entry name" value="ARM-type_fold"/>
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        <property type="entry name" value="SMG1"/>
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        <property type="entry name" value="SMG1"/>
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        <property type="entry name" value="ARM repeat"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000033509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    </evidence>
    <sequence length="652" mass="73430" checksum="3315766DE10DAE6A" modified="2012-04-18" version="1"> </sequence>
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<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>H2XVF9</accession>
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            <fullName evidence="9">Membrane protein BRI3</fullName>
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            <fullName evidence="10">Brain protein I3</fullName>
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        <name evidence="13" type="primary">LOC100176190</name>
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    <organism evidence="13 14">
        <name type="scientific">Ciona intestinalis</name>
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            <taxon>Ciona</taxon>
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    <reference evidence="14" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
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                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
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                <person name="Miura S."/>
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                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <text evidence="11">Interacts with BRI3BP. Interacts with MGAT1 and IFITM3.</text>
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    <comment type="subcellular location">
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            <location evidence="1">Lysosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the BRI3 family.</text>
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        <property type="entry name" value="BRI3"/>
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                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01002028">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="H2XWL6"/>
    <dbReference type="STRING" id="7719.ENSCINP00000034050"/>
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        <property type="protein sequence ID" value="ENSCINP00000034050.1"/>
        <property type="gene ID" value="ENSCING00000018767.1"/>
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    <dbReference type="GeneTree" id="ENSGT00550000075207"/>
    <dbReference type="HOGENOM" id="CLU_2151385_0_0_1"/>
    <dbReference type="InParanoid" id="H2XWL6"/>
    <dbReference type="OMA" id="ADETVLW"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
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    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alcohol dehydrogenase-like C-terminal" evidence="1">
        <location>
            <begin position="4"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000034050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    <sequence length="118" mass="12762" checksum="5976F0266CC86060" modified="2012-04-18" version="1">NEIGYDAIIDFVNTRVTSTRAFECLNTDGLQVCVGLFGGSGNFPLPVLTLSEKGVKGVYVGRLQQLKDLIALVSTNKLTPPPLIYYKLEDINSALDLLRVGKINGRGIIKHEGANDDA</sequence>
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        <recommendedName>
            <fullName evidence="4">Round spermatid basic protein 1</fullName>
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    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the round spermatid basic protein 1 family.</text>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="H2XX76"/>
    <dbReference type="STRING" id="7719.ENSCINP00000034260"/>
    <dbReference type="Ensembl" id="ENSCINT00000035200.1">
        <property type="protein sequence ID" value="ENSCINP00000034260.1"/>
        <property type="gene ID" value="ENSCING00000019097.1"/>
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    <dbReference type="InParanoid" id="H2XX76"/>
    <dbReference type="OMA" id="VMGIIHN"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="InterPro" id="IPR026306">
        <property type="entry name" value="RSBN1/Dpy-2/CEP530"/>
    </dbReference>
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        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13354">
        <property type="entry name" value="ROUND SPERMATID BASIC PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000034260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="334" mass="38193" checksum="8512E6AA62F0A6A6" modified="2012-04-18" version="1">MQKKFAHDFCTLCFLEEKNSEADFVMGIVHGSATNMPDFLEYLSEKRGDMRVKSEVLGQRDIVTMNIREFREQVCKTFSVESGMYRYGPMRQISLVGAVHEEAGGYMPDVLDMLEENPFLNITSPWGAMSCVDLECRSLSNDGPILWIRPGEQMVPAADLKGIGSPAKRRRSQVGGLQKLAFQYSARSNDMRETFIEDRTHAHADHVGHITDRQTTWAVGVLKSITAGHKPKMNKLLKDVICFHASSFFNTSQTLQLDLFEPPMSQCVRWVDEAKLNQMRREGIRYSRVKLYDNDIYFIPRNIIHQFRTVSSSSSVAWHMRMKGLHGEPTYIKP</sequence>
</entry>
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    <accession>H2XXR7</accession>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
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    <organism evidence="2 3">
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        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="H2XXR7"/>
    <dbReference type="Ensembl" id="ENSCINT00000032195.1">
        <property type="protein sequence ID" value="ENSCINP00000034451.1"/>
        <property type="gene ID" value="ENSCING00000022623.1"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_180895_1_0_1"/>
    <dbReference type="InParanoid" id="H2XXR7"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003577567" evidence="1">
        <location>
            <begin position="24"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000034451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="69" mass="7892" checksum="05049FAEF4CD138B" modified="2012-04-18" version="1">MNKSAILLLFVIGILVLNESVDALHQWRRIPTSGTLNRLTPARRRWGGWNQMENMNVEDNGLTDWMIGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>H2XXX9</accession>
    <name>H2XXX9_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">UPAR/Ly6 domain-containing protein</fullName>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="H2XXX9"/>
    <dbReference type="Ensembl" id="ENSCINT00000033949.1">
        <property type="protein sequence ID" value="ENSCINP00000034513.1"/>
        <property type="gene ID" value="ENSCING00000022298.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000172303"/>
    <dbReference type="HOGENOM" id="CLU_2195983_0_0_1"/>
    <dbReference type="InParanoid" id="H2XXX9"/>
    <dbReference type="OMA" id="TRCKECN"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23539">
        <property type="entry name" value="TFP_LU_ECD_CinHb4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045860">
        <property type="entry name" value="Snake_toxin-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57302">
        <property type="entry name" value="Snake toxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000034513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="108" mass="12417" checksum="D5B28F993AD9B3D0" modified="2012-04-18" version="1">MYLNYFVRPPRRGLRCLVCYKARSHEECEATGTIKRCLRNEQQCATDIRTLNWGRKEITKYCKQEAACLNERKQNPVDCRNNGPNKLCRCCCGSDLCNMDATLCTGSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>H2XYS7</accession>
    <name>H2XYS7_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">E3 ubiquitin-protein ligase FANCL</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100175446</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
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            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="H2XYS7"/>
    <dbReference type="FunCoup" id="H2XYS7">
        <property type="interactions" value="5"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000034811"/>
    <dbReference type="Ensembl" id="ENSCINT00000035459.1">
        <property type="protein sequence ID" value="ENSCINP00000034811.1"/>
        <property type="gene ID" value="ENSCING00000019870.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000005537"/>
    <dbReference type="HOGENOM" id="CLU_045054_0_0_1"/>
    <dbReference type="InParanoid" id="H2XYS7"/>
    <dbReference type="OMA" id="NRPFHAK"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043240">
        <property type="term" value="C:Fanconi anaemia nuclear complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036297">
        <property type="term" value="P:interstrand cross-link repair"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006513">
        <property type="term" value="P:protein monoubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd23832">
        <property type="entry name" value="DRWD-C_FANCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23831">
        <property type="entry name" value="DRWD-N_FANCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23786">
        <property type="entry name" value="ELF_FANCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16490">
        <property type="entry name" value="RING-CH-C4HC3_FANCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.20">
        <property type="entry name" value="RWD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026848">
        <property type="entry name" value="Fancl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026850">
        <property type="entry name" value="FANCL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043898">
        <property type="entry name" value="FANCL_d2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044037">
        <property type="entry name" value="FANCL_d3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043003">
        <property type="entry name" value="FANCL_d3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019162">
        <property type="entry name" value="FancL_WD-rpt_cont_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13206:SF0">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE FANCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13206">
        <property type="entry name" value="UBIQUITIN LIGASE PROTEIN PHF9 FANCONI ANEMIA GROUP L PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11793">
        <property type="entry name" value="FANCL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FANCL_d1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18890">
        <property type="entry name" value="FANCL_d2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FANCL_d3"/>
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    </dbReference>
    <dbReference type="SMART" id="SM01197">
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="4"/>
            <end position="86"/>
        </location>
    </feature>
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        <location>
            <begin position="100"/>
            <end position="187"/>
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        <location>
            <begin position="191"/>
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        <location>
            <begin position="295"/>
            <end position="360"/>
        </location>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF11793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF18890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF18891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000034811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="366" mass="42415" checksum="94A4D7E2F2668519" modified="2012-04-18" version="1">KPTLLQMNSPIALNSSKTVYDGFVKHGEKFYRLQVRGEAGQSSVKYRVCCDWKLRHILRDHLPLLNERLQSCADVSTFISDVKTILGVQSDQHSPSHCYKHYGRVLKEIEEVGWDKLNNISEDFKLIKFQFQDNSERWHYFGLKLNDNYPQESPEFMVDLPCPFIPVWACGSNLMTLCQQFIEVLDLHQSLWDMVDELKDKCWVLEPEQPNYASTSFRIALEQTTSVVIDINPLHPEEIPDCKFLGSETSVAKLEEKYQQGYQEWSDSLSLYQNIQMLFNINFHKPPNTTSMESNLQCGICYGYRLGTQTPTQVCNDERCNQIYHHDCLVQWLQSIPGVKRSFGIIFGECPYCSNNIKLKILQKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>H2XZ94</accession>
    <name>H2XZ94_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Phlebobranchia</taxon>
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        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
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                <person name="Matsumoto J."/>
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                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="De Tomaso A."/>
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                <person name="Hastings K.E."/>
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    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000035286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="137" mass="15611" checksum="1C1D74E6F171C140" modified="2012-04-18" version="1">STGGSPFVLSESSKLPALLPALPEDQKKSPGDFHQITSHRVAAKLHRCDHCGHIFPTVQAFTRHKRACNKYECPICHRGFSQVGNMRRHMKIFHSDQKPFECKVCNKAYGIQQSFRYHMRDKHGIILGKPNSKAEEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>H2Y0A4</accession>
    <name>H2Y0A4_CIOIN</name>
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        <recommendedName>
            <fullName evidence="2">PGAP2IP first transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2Y0A4"/>
    <dbReference type="Ensembl" id="ENSCINT00000035169.1">
        <property type="protein sequence ID" value="ENSCINP00000035338.1"/>
        <property type="gene ID" value="ENSCING00000022050.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00510000048509"/>
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    <dbReference type="InParanoid" id="H2Y0A4"/>
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        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053912">
        <property type="entry name" value="PGAP2IP_TM_1nd"/>
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    <dbReference type="Pfam" id="PF23022">
        <property type="entry name" value="6TM_1st_PGAP2IP"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="16"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="128"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="PGAP2IP first transmembrane" evidence="2">
        <location>
            <begin position="19"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000035338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="159" mass="17976" checksum="7F2BB3C0A3C75966" modified="2012-04-18" version="1">MKPIGSFKTQLACESMIGYTYWSLVHGLGPMIWFYPLNELDITGYELFVVVAFSPMLFLFGEGARSFLSKKAVVNFFLLLMLVGASSFHASSTLLRLVGLSVGVGVAFIVQCATWWSGTEQQRRIHFWGYVLGFFALVASRIWYLSMTPLWNDHKSNKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>H2Y100</accession>
    <name>H2Y100_CIOIN</name>
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        <recommendedName>
            <fullName evidence="4">Trafficking protein particle complex subunit 11</fullName>
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    </protein>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
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    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
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            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">wild type</strain>
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    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="10" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="1">Involved in endoplasmic reticulum to Golgi apparatus trafficking at a very early stage.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
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            <location evidence="2">cis-Golgi network</location>
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    <comment type="similarity">
        <text evidence="3">Belongs to the TRAPPC11 family.</text>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
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    <dbReference type="Ensembl" id="ENSCINT00000036297.1">
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        <property type="gene ID" value="ENSCING00000023712.1"/>
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        <property type="component" value="Chromosome 12"/>
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    <dbReference type="GO" id="GO:0005794">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="TPC11"/>
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    <dbReference type="InterPro" id="IPR025876">
        <property type="entry name" value="TRAPPC11_C"/>
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    <dbReference type="PANTHER" id="PTHR14374">
        <property type="entry name" value="FOIE GRAS"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
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        <source>
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    <accession>H2Y159</accession>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
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            <taxon>Phlebobranchia</taxon>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">wild type</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="RefSeq" id="XP_026691985.1">
        <property type="nucleotide sequence ID" value="XM_026836184.1"/>
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    <dbReference type="AlphaFoldDB" id="H2Y159"/>
    <dbReference type="Ensembl" id="ENSCINT00000030863.1">
        <property type="protein sequence ID" value="ENSCINP00000035643.1"/>
        <property type="gene ID" value="ENSCING00000018470.1"/>
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    <dbReference type="GeneID" id="108949271"/>
    <dbReference type="GeneTree" id="ENSGT00650000093597"/>
    <dbReference type="HOGENOM" id="CLU_042050_0_0_1"/>
    <dbReference type="InParanoid" id="H2Y159"/>
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        <property type="component" value="Chromosome 9"/>
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        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10036">
        <property type="entry name" value="CD59 GLYCOPROTEIN"/>
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        <property type="entry name" value="HEP21 PROTEIN"/>
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        <property type="match status" value="1"/>
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        <source>
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    <reference evidence="7" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">wild type</strain>
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    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="6" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <dbReference type="KEGG" id="cin:100185496"/>
    <dbReference type="GeneTree" id="ENSGT00940000166853"/>
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    <dbReference type="GO" id="GO:0005886">
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    <reference evidence="13" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">wild type</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the transition zone in primary cilia. Required for ciliogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TMEM237 family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002976">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2Y261"/>
    <dbReference type="FunCoup" id="H2Y261">
        <property type="interactions" value="5"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSCINT00000035142.1">
        <property type="protein sequence ID" value="ENSCINP00000035996.1"/>
        <property type="gene ID" value="ENSCING00000019821.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00660000097199"/>
    <dbReference type="HOGENOM" id="CLU_1087888_0_0_1"/>
    <dbReference type="InParanoid" id="H2Y261"/>
    <dbReference type="OMA" id="QPAMENS"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030030">
        <property type="term" value="P:cell projection organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029409">
        <property type="entry name" value="TMEM237"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28388">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 237"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28388:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 237"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15383">
        <property type="entry name" value="TMEM237"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0969">Cilium</keyword>
    <keyword evidence="5" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="217"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="9"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="29"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="60"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="89"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="102"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000035996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="256" mass="27801" checksum="CDB89CB9A796EE8F" modified="2012-04-18" version="1">MADSTAEATSSLTLRPLPRRALPPLTSGEAASTTSMVESLNEKPTSENVTPRKKRRKRQNISASETPTESPSRSLPPISAAATTQDTLNPNTSNTSTASKTARARRRQGNRKPRKTPSPEVITNGQAITNMVNEEDVVAVEHIEDMVSMSTLPGIPGAGGANSQAQDRVYVQTTSGFKSHTDDWLASRMNKANQRSDTEDASHVTGSEEVALSFHRAFLFLTFLLHGLLAGLTVMQMSIAFVLRGPGDENFFEQYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>H2Y3E5</accession>
    <name>H2Y3E5_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Fatty acid synthase</fullName>
            <ecNumber evidence="5">1.1.1.100</ecNumber>
            <ecNumber evidence="2">1.3.1.39</ecNumber>
            <ecNumber evidence="6">2.3.1.41</ecNumber>
            <ecNumber evidence="4">2.3.1.85</ecNumber>
            <ecNumber evidence="3">3.1.2.14</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="68 69">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="69" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="68" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="68">wild type</strain>
        </source>
    </reference>
    <reference evidence="68" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="68" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="32">Fatty acid synthetase is a multifunctional enzyme that catalyzes the de novo biosynthesis of long-chain saturated fatty acids starting from acetyl-CoA and malonyl-CoA in the presence of NADPH. This multifunctional protein contains 7 catalytic activities and a site for the binding of the prosthetic group 4'-phosphopantetheine of the acyl carrier protein ([ACP]) domain.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="39">
            <text>(2E)-butenoyl-[ACP] + NADPH + H(+) = butanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41812"/>
            <dbReference type="Rhea" id="RHEA-COMP:9627"/>
            <dbReference type="Rhea" id="RHEA-COMP:9628"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78453"/>
            <dbReference type="ChEBI" id="CHEBI:78454"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="39">
            <dbReference type="Rhea" id="RHEA:41813"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="63">
            <text>(2E)-decenoyl-[ACP] + NADPH + H(+) = decanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41864"/>
            <dbReference type="Rhea" id="RHEA-COMP:9639"/>
            <dbReference type="Rhea" id="RHEA-COMP:9640"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78467"/>
            <dbReference type="ChEBI" id="CHEBI:78468"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="63">
            <dbReference type="Rhea" id="RHEA:41865"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="46">
            <text>(2E)-dodecenoyl-[ACP] + NADPH + H(+) = dodecanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41880"/>
            <dbReference type="Rhea" id="RHEA-COMP:9643"/>
            <dbReference type="Rhea" id="RHEA-COMP:9644"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:65264"/>
            <dbReference type="ChEBI" id="CHEBI:78472"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="46">
            <dbReference type="Rhea" id="RHEA:41881"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="41">
            <text>(2E)-hexadecenoyl-[ACP] + NADPH + H(+) = hexadecanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41912"/>
            <dbReference type="Rhea" id="RHEA-COMP:9651"/>
            <dbReference type="Rhea" id="RHEA-COMP:9652"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78481"/>
            <dbReference type="ChEBI" id="CHEBI:78483"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="41">
            <dbReference type="Rhea" id="RHEA:41913"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="42">
            <text>(2E)-hexenoyl-[ACP] + NADPH + H(+) = hexanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41832"/>
            <dbReference type="Rhea" id="RHEA-COMP:9631"/>
            <dbReference type="Rhea" id="RHEA-COMP:9632"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78458"/>
            <dbReference type="ChEBI" id="CHEBI:78459"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="42">
            <dbReference type="Rhea" id="RHEA:41833"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="56">
            <text>(2E)-octadecenoyl-[ACP] + NADPH + H(+) = octadecanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41928"/>
            <dbReference type="Rhea" id="RHEA-COMP:9655"/>
            <dbReference type="Rhea" id="RHEA-COMP:9656"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78489"/>
            <dbReference type="ChEBI" id="CHEBI:78495"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="56">
            <dbReference type="Rhea" id="RHEA:41929"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="49">
            <text>(2E)-octenoyl-[ACP] + NADPH + H(+) = octanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41848"/>
            <dbReference type="Rhea" id="RHEA-COMP:9635"/>
            <dbReference type="Rhea" id="RHEA-COMP:9636"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78462"/>
            <dbReference type="ChEBI" id="CHEBI:78463"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="49">
            <dbReference type="Rhea" id="RHEA:41849"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="58">
            <text>(2E)-tetradecenoyl-[ACP] + NADPH + H(+) = tetradecanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41896"/>
            <dbReference type="Rhea" id="RHEA-COMP:9647"/>
            <dbReference type="Rhea" id="RHEA-COMP:9648"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78475"/>
            <dbReference type="ChEBI" id="CHEBI:78477"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="58">
            <dbReference type="Rhea" id="RHEA:41897"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="31">
            <text>(3R)-hydroxybutanoyl-[ACP] = (2E)-butenoyl-[ACP] + H2O</text>
            <dbReference type="Rhea" id="RHEA:41808"/>
            <dbReference type="Rhea" id="RHEA-COMP:9626"/>
            <dbReference type="Rhea" id="RHEA-COMP:9627"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:78451"/>
            <dbReference type="ChEBI" id="CHEBI:78453"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="31">
            <dbReference type="Rhea" id="RHEA:41809"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>(3R)-hydroxydecanoyl-[ACP] = (2E)-decenoyl-[ACP] + H2O</text>
            <dbReference type="Rhea" id="RHEA:41860"/>
            <dbReference type="Rhea" id="RHEA-COMP:9638"/>
            <dbReference type="Rhea" id="RHEA-COMP:9639"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:78466"/>
            <dbReference type="ChEBI" id="CHEBI:78467"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:41861"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>(3R)-hydroxydodecanoyl-[ACP] = (2E)-dodecenoyl-[ACP] + H2O</text>
            <dbReference type="Rhea" id="RHEA:41876"/>
            <dbReference type="Rhea" id="RHEA-COMP:9642"/>
            <dbReference type="Rhea" id="RHEA-COMP:9643"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:78470"/>
            <dbReference type="ChEBI" id="CHEBI:78472"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:41877"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="30">
            <text>(3R)-hydroxyhexadecanoyl-[ACP] = (2E)-hexadecenoyl-[ACP] + H2O</text>
            <dbReference type="Rhea" id="RHEA:41908"/>
            <dbReference type="Rhea" id="RHEA-COMP:9650"/>
            <dbReference type="Rhea" id="RHEA-COMP:9651"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:78480"/>
            <dbReference type="ChEBI" id="CHEBI:78481"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="30">
            <dbReference type="Rhea" id="RHEA:41909"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>(3R)-hydroxyhexanoyl-[ACP] = (2E)-hexenoyl-[ACP] + H2O</text>
            <dbReference type="Rhea" id="RHEA:41828"/>
            <dbReference type="Rhea" id="RHEA-COMP:9630"/>
            <dbReference type="Rhea" id="RHEA-COMP:9631"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:78457"/>
            <dbReference type="ChEBI" id="CHEBI:78458"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:41829"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>(3R)-hydroxyoctadecanoyl-[ACP] = (2E)-octadecenoyl-[ACP] + H2O</text>
            <dbReference type="Rhea" id="RHEA:41924"/>
            <dbReference type="Rhea" id="RHEA-COMP:9654"/>
            <dbReference type="Rhea" id="RHEA-COMP:9655"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:78488"/>
            <dbReference type="ChEBI" id="CHEBI:78489"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="29">
            <dbReference type="Rhea" id="RHEA:41925"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>(3R)-hydroxyoctanoyl-[ACP] = (2E)-octenoyl-[ACP] + H2O</text>
            <dbReference type="Rhea" id="RHEA:41844"/>
            <dbReference type="Rhea" id="RHEA-COMP:9634"/>
            <dbReference type="Rhea" id="RHEA-COMP:9635"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:78461"/>
            <dbReference type="ChEBI" id="CHEBI:78462"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:41845"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>(3R)-hydroxytetradecanoyl-[ACP] = (2E)-tetradecenoyl-[ACP] + H2O</text>
            <dbReference type="Rhea" id="RHEA:41892"/>
            <dbReference type="Rhea" id="RHEA-COMP:9646"/>
            <dbReference type="Rhea" id="RHEA-COMP:9647"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:78474"/>
            <dbReference type="ChEBI" id="CHEBI:78475"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="28">
            <dbReference type="Rhea" id="RHEA:41893"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="43">
            <text>3-oxobutanoyl-[ACP] + NADPH + H(+) = (3R)-hydroxybutanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41804"/>
            <dbReference type="Rhea" id="RHEA-COMP:9625"/>
            <dbReference type="Rhea" id="RHEA-COMP:9626"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78450"/>
            <dbReference type="ChEBI" id="CHEBI:78451"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="43">
            <dbReference type="Rhea" id="RHEA:41805"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="37">
            <text>3-oxodecanoyl-[ACP] + NADPH + H(+) = (3R)-hydroxydecanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41856"/>
            <dbReference type="Rhea" id="RHEA-COMP:9637"/>
            <dbReference type="Rhea" id="RHEA-COMP:9638"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78464"/>
            <dbReference type="ChEBI" id="CHEBI:78466"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="37">
            <dbReference type="Rhea" id="RHEA:41857"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="59">
            <text>3-oxododecanoyl-[ACP] + NADPH + H(+) = (3R)-hydroxydodecanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41872"/>
            <dbReference type="Rhea" id="RHEA-COMP:9641"/>
            <dbReference type="Rhea" id="RHEA-COMP:9642"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78469"/>
            <dbReference type="ChEBI" id="CHEBI:78470"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="59">
            <dbReference type="Rhea" id="RHEA:41873"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="60">
            <text>3-oxohexadecanoyl-[ACP] + NADPH + H(+) = (3R)-hydroxyhexadecanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41904"/>
            <dbReference type="Rhea" id="RHEA-COMP:9649"/>
            <dbReference type="Rhea" id="RHEA-COMP:9650"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78478"/>
            <dbReference type="ChEBI" id="CHEBI:78480"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="60">
            <dbReference type="Rhea" id="RHEA:41905"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="51">
            <text>3-oxohexanoyl-[ACP] + NADPH + H(+) = (3R)-hydroxyhexanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41824"/>
            <dbReference type="Rhea" id="RHEA-COMP:9629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9630"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78456"/>
            <dbReference type="ChEBI" id="CHEBI:78457"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="51">
            <dbReference type="Rhea" id="RHEA:41825"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="34">
            <text>3-oxooctadecanoyl-[ACP] + NADPH + H(+) = (3R)-hydroxyoctadecanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41920"/>
            <dbReference type="Rhea" id="RHEA-COMP:9653"/>
            <dbReference type="Rhea" id="RHEA-COMP:9654"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78487"/>
            <dbReference type="ChEBI" id="CHEBI:78488"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="34">
            <dbReference type="Rhea" id="RHEA:41921"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="61">
            <text>3-oxooctanoyl-[ACP] + NADPH + H(+) = (3R)-hydroxyoctanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41840"/>
            <dbReference type="Rhea" id="RHEA-COMP:9633"/>
            <dbReference type="Rhea" id="RHEA-COMP:9634"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78460"/>
            <dbReference type="ChEBI" id="CHEBI:78461"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="61">
            <dbReference type="Rhea" id="RHEA:41841"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="55">
            <text>3-oxotetradecanoyl-[ACP] + NADPH + H(+) = (3R)-hydroxytetradecanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41888"/>
            <dbReference type="Rhea" id="RHEA-COMP:9645"/>
            <dbReference type="Rhea" id="RHEA-COMP:9646"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78473"/>
            <dbReference type="ChEBI" id="CHEBI:78474"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="55">
            <dbReference type="Rhea" id="RHEA:41889"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="36">
            <text>a (3R)-hydroxyacyl-[ACP] + NADP(+) = a 3-oxoacyl-[ACP] + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17397"/>
            <dbReference type="Rhea" id="RHEA-COMP:9916"/>
            <dbReference type="Rhea" id="RHEA-COMP:9945"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78776"/>
            <dbReference type="ChEBI" id="CHEBI:78827"/>
            <dbReference type="EC" id="1.1.1.100"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="36">
            <dbReference type="Rhea" id="RHEA:17399"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>a (3R)-hydroxyacyl-[ACP] = a (2E)-enoyl-[ACP] + H2O</text>
            <dbReference type="Rhea" id="RHEA:13097"/>
            <dbReference type="Rhea" id="RHEA-COMP:9925"/>
            <dbReference type="Rhea" id="RHEA-COMP:9945"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:78784"/>
            <dbReference type="ChEBI" id="CHEBI:78827"/>
            <dbReference type="EC" id="4.2.1.59"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="27">
            <dbReference type="Rhea" id="RHEA:13098"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="52">
            <text>a 2,3-saturated acyl-[ACP] + NADP(+) = a (2E)-enoyl-[ACP] + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22564"/>
            <dbReference type="Rhea" id="RHEA-COMP:9925"/>
            <dbReference type="Rhea" id="RHEA-COMP:9926"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78784"/>
            <dbReference type="ChEBI" id="CHEBI:78785"/>
            <dbReference type="EC" id="1.3.1.39"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="52">
            <dbReference type="Rhea" id="RHEA:22566"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="50">
            <text>a fatty acyl-[ACP] + malonyl-[ACP] + H(+) = a 3-oxoacyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:22836"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:9916"/>
            <dbReference type="Rhea" id="RHEA-COMP:14125"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78776"/>
            <dbReference type="ChEBI" id="CHEBI:138651"/>
            <dbReference type="EC" id="2.3.1.41"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="50">
            <dbReference type="Rhea" id="RHEA:22837"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="33">
            <text>acetyl-CoA + n malonyl-CoA + 2n NADPH + 2n H(+) = a long-chain fatty acid + (n+1) CoA + n CO2 + 2n NADP(+).</text>
            <dbReference type="EC" id="2.3.1.85"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="44">
            <text>acetyl-[ACP] + malonyl-[ACP] + H(+) = 3-oxobutanoyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:41800"/>
            <dbReference type="Rhea" id="RHEA-COMP:9621"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9625"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78446"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78450"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="44">
            <dbReference type="Rhea" id="RHEA:41801"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="62">
            <text>butanoyl-[ACP] + malonyl-[ACP] + H(+) = 3-oxohexanoyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:41820"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9628"/>
            <dbReference type="Rhea" id="RHEA-COMP:9629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78454"/>
            <dbReference type="ChEBI" id="CHEBI:78456"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="62">
            <dbReference type="Rhea" id="RHEA:41821"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="57">
            <text>decanoyl-[ACP] + malonyl-[ACP] + H(+) = 3-oxododecanoyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:41868"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9640"/>
            <dbReference type="Rhea" id="RHEA-COMP:9641"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78468"/>
            <dbReference type="ChEBI" id="CHEBI:78469"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="57">
            <dbReference type="Rhea" id="RHEA:41869"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="40">
            <text>dodecanoyl-[ACP] + malonyl-[ACP] + H(+) = 3-oxotetradecanoyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:41884"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9644"/>
            <dbReference type="Rhea" id="RHEA-COMP:9645"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:65264"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78473"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="40">
            <dbReference type="Rhea" id="RHEA:41885"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="54">
            <text>hexadecanoyl-[ACP] + H2O = hexadecanoate + holo-[ACP] + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41932"/>
            <dbReference type="Rhea" id="RHEA-COMP:9652"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78483"/>
            <dbReference type="EC" id="3.1.2.14"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="54">
            <dbReference type="Rhea" id="RHEA:41933"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="45">
            <text>hexadecanoyl-[ACP] + malonyl-[ACP] + H(+) = 3-oxooctadecanoyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:41916"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9652"/>
            <dbReference type="Rhea" id="RHEA-COMP:9653"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78483"/>
            <dbReference type="ChEBI" id="CHEBI:78487"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="45">
            <dbReference type="Rhea" id="RHEA:41917"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="35">
            <text>hexanoyl-[ACP] + malonyl-[ACP] + H(+) = 3-oxooctanoyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:41836"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9632"/>
            <dbReference type="Rhea" id="RHEA-COMP:9633"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78459"/>
            <dbReference type="ChEBI" id="CHEBI:78460"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="35">
            <dbReference type="Rhea" id="RHEA:41837"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="53">
            <text>holo-[ACP] + acetyl-CoA = acetyl-[ACP] + CoA</text>
            <dbReference type="Rhea" id="RHEA:41788"/>
            <dbReference type="Rhea" id="RHEA-COMP:9621"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78446"/>
            <dbReference type="EC" id="2.3.1.38"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="53">
            <dbReference type="Rhea" id="RHEA:41789"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="48">
            <text>holo-[ACP] + malonyl-CoA = malonyl-[ACP] + CoA</text>
            <dbReference type="Rhea" id="RHEA:41792"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
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            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="EC" id="2.3.1.39"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="48">
            <dbReference type="Rhea" id="RHEA:41793"/>
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    </comment>
    <comment type="catalytic activity">
        <reaction evidence="64">
            <text>octanoyl-[ACP] + malonyl-[ACP] + H(+) = 3-oxodecanoyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:41852"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
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            <dbReference type="ChEBI" id="CHEBI:78463"/>
            <dbReference type="ChEBI" id="CHEBI:78464"/>
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            <dbReference type="Rhea" id="RHEA:41853"/>
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    </comment>
    <comment type="catalytic activity">
        <reaction evidence="47">
            <text>tetradecanoyl-[ACP] + H2O = tetradecanoate + holo-[ACP] + H(+)</text>
            <dbReference type="Rhea" id="RHEA:30123"/>
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            <dbReference type="ChEBI" id="CHEBI:78477"/>
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            <dbReference type="Rhea" id="RHEA:30124"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="38">
            <text>tetradecanoyl-[ACP] + malonyl-[ACP] + H(+) = 3-oxohexadecanoyl-[ACP] + holo-[ACP] + CO2</text>
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            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
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            <dbReference type="ChEBI" id="CHEBI:78478"/>
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        <physiologicalReaction direction="left-to-right" evidence="38">
            <dbReference type="Rhea" id="RHEA:41901"/>
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    </comment>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="EMBL" id="EAAA01001926">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="interactions" value="2"/>
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        <property type="protein sequence ID" value="ENSCINP00000036430.1"/>
        <property type="gene ID" value="ENSCING00000019644.1"/>
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    <dbReference type="GeneTree" id="ENSGT00940000157276"/>
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    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 4"/>
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        <property type="term" value="C:cytoplasm"/>
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        <property type="project" value="GO_Central"/>
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        <property type="term" value="F:(3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity"/>
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        <property type="term" value="F:[acyl-carrier-protein] S-acetyltransferase activity"/>
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        <property type="term" value="F:fatty acyl-[ACP] hydrolase activity"/>
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        <property type="entry name" value="enoyl_red"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="PKS"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Malonyl-Coenzyme A Acyl Carrier Protein, domain 2"/>
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    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
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    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.110">
        <property type="entry name" value="Polyketide synthase dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001227">
        <property type="entry name" value="Ac_transferase_dom_sf"/>
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    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
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    <dbReference type="InterPro" id="IPR014043">
        <property type="entry name" value="Acyl_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049391">
        <property type="entry name" value="FAS_pseudo-KR"/>
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    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
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    <dbReference type="InterPro" id="IPR018201">
        <property type="entry name" value="Ketoacyl_synth_AS"/>
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    <dbReference type="InterPro" id="IPR014031">
        <property type="entry name" value="Ketoacyl_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014030">
        <property type="entry name" value="Ketoacyl_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057326">
        <property type="entry name" value="KR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016036">
        <property type="entry name" value="Malonyl_transacylase_ACP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032821">
        <property type="entry name" value="PKS_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020841">
        <property type="entry name" value="PKS_Beta-ketoAc_synthase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042104">
        <property type="entry name" value="PKS_dehydratase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049552">
        <property type="entry name" value="PKS_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013968">
        <property type="entry name" value="PKS_KR"/>
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    <dbReference type="InterPro" id="IPR049900">
        <property type="entry name" value="PKS_mFAS_DH"/>
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    <dbReference type="InterPro" id="IPR050091">
        <property type="entry name" value="PKS_NRPS_Biosynth_Enz"/>
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    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
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    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
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    <dbReference type="InterPro" id="IPR001031">
        <property type="entry name" value="Thioesterase"/>
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    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
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    <dbReference type="PANTHER" id="PTHR43775">
        <property type="entry name" value="FATTY ACID SYNTHASE"/>
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    </dbReference>
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        <property type="entry name" value="FATTY ACID SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00698">
        <property type="entry name" value="Acyl_transf_1"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF21149">
        <property type="entry name" value="FAS_pseudo-KR"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF16197">
        <property type="entry name" value="KAsynt_C_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00109">
        <property type="entry name" value="ketoacyl-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02801">
        <property type="entry name" value="Ketoacyl-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08659">
        <property type="entry name" value="KR"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF21089">
        <property type="entry name" value="PKS_DH_N"/>
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    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00975">
        <property type="entry name" value="Thioesterase"/>
        <property type="match status" value="1"/>
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    <dbReference type="SMART" id="SM00827">
        <property type="entry name" value="PKS_AT"/>
        <property type="match status" value="1"/>
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    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PKS_KR"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PKS_KS"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="ACP-like"/>
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    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="entry name" value="Probable ACP-binding domain of malonyl-CoA ACP transacylase"/>
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    </dbReference>
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        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="KS3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="KS3_2"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS52019">
        <property type="entry name" value="PKS_MFAS_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="17" id="KW-0007">Acetylation</keyword>
    <keyword evidence="21" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="14" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="20" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="22" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="19" id="KW-0520">NAD</keyword>
    <keyword evidence="15" id="KW-0521">NADP</keyword>
    <keyword evidence="18" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="10" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="69" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0702">S-nitrosylation</keyword>
    <keyword evidence="11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ketosynthase family 3 (KS3)" evidence="66">
        <location>
            <begin position="1"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="domain" description="PKS/mFAS DH" evidence="67">
        <location>
            <begin position="845"/>
            <end position="1121"/>
        </location>
    </feature>
    <feature type="region of interest" description="N-terminal hotdog fold" evidence="65">
        <location>
            <begin position="845"/>
            <end position="970"/>
        </location>
    </feature>
    <feature type="region of interest" description="C-terminal hotdog fold" evidence="65">
        <location>
            <begin position="981"/>
            <end position="1121"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for dehydratase activity" evidence="65">
        <location>
            <position position="883"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor; for dehydratase activity" evidence="65">
        <location>
            <position position="1030"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012004"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00018769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00023351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00023373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00023388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00023394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00023398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00023399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00023401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00023402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00023442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="ARBA" id="ARBA00044883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="ARBA" id="ARBA00047300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="35">
        <source>
            <dbReference type="ARBA" id="ARBA00047394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="36">
        <source>
            <dbReference type="ARBA" id="ARBA00047400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="37">
        <source>
            <dbReference type="ARBA" id="ARBA00047440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="38">
        <source>
            <dbReference type="ARBA" id="ARBA00047451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="39">
        <source>
            <dbReference type="ARBA" id="ARBA00047500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="40">
        <source>
            <dbReference type="ARBA" id="ARBA00047578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="41">
        <source>
            <dbReference type="ARBA" id="ARBA00047810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="42">
        <source>
            <dbReference type="ARBA" id="ARBA00047897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="43">
        <source>
            <dbReference type="ARBA" id="ARBA00047953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="44">
        <source>
            <dbReference type="ARBA" id="ARBA00047961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="45">
        <source>
            <dbReference type="ARBA" id="ARBA00048051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="46">
        <source>
            <dbReference type="ARBA" id="ARBA00048281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="47">
        <source>
            <dbReference type="ARBA" id="ARBA00048289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="48">
        <source>
            <dbReference type="ARBA" id="ARBA00048404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="49">
        <source>
            <dbReference type="ARBA" id="ARBA00048420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="50">
        <source>
            <dbReference type="ARBA" id="ARBA00048506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="51">
        <source>
            <dbReference type="ARBA" id="ARBA00048571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="52">
        <source>
            <dbReference type="ARBA" id="ARBA00048650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="53">
        <source>
            <dbReference type="ARBA" id="ARBA00048691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="54">
        <source>
            <dbReference type="ARBA" id="ARBA00048704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="55">
        <source>
            <dbReference type="ARBA" id="ARBA00048935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="56">
        <source>
            <dbReference type="ARBA" id="ARBA00049019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="57">
        <source>
            <dbReference type="ARBA" id="ARBA00049109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="58">
        <source>
            <dbReference type="ARBA" id="ARBA00049171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="59">
        <source>
            <dbReference type="ARBA" id="ARBA00049263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="60">
        <source>
            <dbReference type="ARBA" id="ARBA00049414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="61">
        <source>
            <dbReference type="ARBA" id="ARBA00049422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="62">
        <source>
            <dbReference type="ARBA" id="ARBA00049449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="63">
        <source>
            <dbReference type="ARBA" id="ARBA00049521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="64">
        <source>
            <dbReference type="ARBA" id="ARBA00049533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="65">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="66">
        <source>
            <dbReference type="PROSITE" id="PS52004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="67">
        <source>
            <dbReference type="PROSITE" id="PS52019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="68">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000036430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="69">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="2428" mass="271447" checksum="1030F7B4F1577D46" modified="2012-04-18" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-12-14" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q1RLC9</accession>
    <name>RU1C_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">U1 small nuclear ribonucleoprotein C</fullName>
            <shortName evidence="1">U1 snRNP C</shortName>
            <shortName evidence="1">U1-C</shortName>
            <shortName evidence="1">U1C</shortName>
        </recommendedName>
    </protein>
    <organism>
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2006" name="Dev. Biol." volume="292" first="546" last="554">
            <title>Systematic analysis of embryonic expression profiles of zinc finger genes in Ciona intestinalis.</title>
            <authorList>
                <person name="Miwata K."/>
                <person name="Chiba T."/>
                <person name="Horii R."/>
                <person name="Yamada L."/>
                <person name="Kubo A."/>
                <person name="Miyamura D."/>
                <person name="Satoh N."/>
                <person name="Satou Y."/>
            </authorList>
            <dbReference type="PubMed" id="16519883"/>
            <dbReference type="DOI" id="10.1016/j.ydbio.2006.01.024"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the spliceosomal U1 snRNP, which is essential for recognition of the pre-mRNA 5' splice-site and the subsequent assembly of the spliceosome. U1-C is directly involved in initial 5' splice-site recognition for both constitutive and regulated alternative splicing. The interaction with the 5' splice-site seems to precede base-pairing between the pre-mRNA and the U1 snRNA. Stimulates commitment or early (E) complex formation by stabilizing the base pairing of the 5' end of the U1 snRNA and the 5' splice-site region.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">U1 snRNP is composed of the 7 core Sm proteins B/B', D1, D2, D3, E, F and G that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP, and at least 3 U1 snRNP-specific proteins U1-70K, U1-A and U1-C. U1-C interacts with U1 snRNA and the 5' splice-site region of the pre-mRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the U1 small nuclear ribonucleoprotein C family.</text>
    </comment>
    <dbReference type="EMBL" id="BR000105">
        <property type="protein sequence ID" value="FAA00136.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001041454.1">
        <property type="nucleotide sequence ID" value="NM_001047989.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1RLC9"/>
    <dbReference type="SMR" id="Q1RLC9"/>
    <dbReference type="FunCoup" id="Q1RLC9">
        <property type="interactions" value="75"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000035879"/>
    <dbReference type="Ensembl" id="ENSCINT00000035913.1">
        <property type="protein sequence ID" value="ENSCINP00000035879.1"/>
        <property type="gene ID" value="ENSCING00000021229.1"/>
    </dbReference>
    <dbReference type="GeneID" id="723802"/>
    <dbReference type="KEGG" id="cin:723802"/>
    <dbReference type="CTD" id="723802"/>
    <dbReference type="eggNOG" id="KOG3454">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00730000110997"/>
    <dbReference type="HOGENOM" id="CLU_079697_3_1_1"/>
    <dbReference type="InParanoid" id="Q1RLC9"/>
    <dbReference type="OMA" id="MMPTGPR"/>
    <dbReference type="OrthoDB" id="76567at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000243">
        <property type="term" value="C:commitment complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030627">
        <property type="term" value="F:pre-mRNA 5'-splice site binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030619">
        <property type="term" value="F:U1 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000395">
        <property type="term" value="P:mRNA 5'-splice site recognition"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000387">
        <property type="term" value="P:spliceosomal snRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000059">
        <property type="entry name" value="U1 small nuclear ribonucleoprotein C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03153">
        <property type="entry name" value="U1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000690">
        <property type="entry name" value="Matrin/U1-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013085">
        <property type="entry name" value="U1-CZ_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017340">
        <property type="entry name" value="U1_snRNP-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31148">
        <property type="entry name" value="U1 SMALL NUCLEAR RIBONUCLEOPROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31148:SF1">
        <property type="entry name" value="U1 SMALL NUCLEAR RIBONUCLEOPROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06220">
        <property type="entry name" value="zf-U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037969">
        <property type="entry name" value="U1_snRNP-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50171">
        <property type="entry name" value="ZF_MATRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0539">Nucleus</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <keyword id="KW-0863">Zinc-finger</keyword>
    <feature type="chain" id="PRO_0000414257" description="U1 small nuclear ribonucleoprotein C">
        <location>
            <begin position="1"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="Matrin-type" evidence="1">
        <location>
            <begin position="4"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03153"/>
        </source>
    </evidence>
    <sequence length="122" mass="13714" checksum="FD0B9162B87BA509" modified="2006-05-16" version="1">MPKYFCDYCDTYLTHDSPSVRKTHCSGRKHKDNVKMYYQTWMEEQAQELIDKTTQAFQKGKMGNFGGLPPNGMMMGPRGPMPMMPMMPVRPMGMGGMLPMRPVPPAMMGGGQVMMMGPRPGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-08-30" modified="2026-01-28" version="140" xmlns="https://uniprot.org/uniprot">
    <accession>Q4H3K3</accession>
    <accession>A0A1W2VP14</accession>
    <name>Q4H3K3_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="9 10">Transcription factor protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">Ci-Fli/ERG2</name>
        <name evidence="10" type="synonym">fli/erg2</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
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                <person name="Wada S."/>
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                <person name="Larimer F."/>
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                <person name="Doggett N."/>
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                <person name="Richardson P."/>
                <person name="Lucas S."/>
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            <title>Genomewide surveys of developmentally relevant genes in Ciona intestinalis.</title>
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                <person name="Satoh N."/>
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            <title>Gene expression profiles of transcription factors and signaling molecules in the ascidian embryo: towards a comprehensive understanding of gene networks.</title>
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                <person name="Hino K."/>
                <person name="Yagi K."/>
                <person name="Satoh N."/>
                <person name="Satou Y."/>
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            <dbReference type="DOI" id="10.1242/dev.01270"/>
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        <citation type="submission" date="2005-04" db="EMBL/GenBank/DDBJ databases">
            <title>Expressed genes in Ciona intestinalis.</title>
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                <person name="Satou Y."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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        </citation>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
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                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
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                <person name="Shin-i T."/>
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                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019985">
        <property type="term" value="P:translesion synthesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040444">
        <property type="entry name" value="PCNA-AF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031444">
        <property type="entry name" value="PCNA-AF_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15679">
        <property type="entry name" value="PCNA-ASSOCIATED FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15679:SF8">
        <property type="entry name" value="PCNA-ASSOCIATED FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15715">
        <property type="entry name" value="PAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCNA-associated factor histone-like" evidence="11">
        <location>
            <begin position="1"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="10"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="67"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="83"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="152"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF15715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000022769.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="213" mass="22683" checksum="DF9D74AA03501666" modified="2017-06-07" version="1">MVRTKATVNSAAFRASAAKAPRKNVAAPSRSSAFEIGSPAGKKNQSVGGNPVCQRPTPDWQKPIASFFTTKLPSAKSNGDTDLVEKLFEPVDKETPDEPEKMETSDDKENQNPEVSDEAGPSTSRPTKTKRRRILTIQESDSETEEVLSGEKPSTSGEKPSTSGEKPSTSGEKPSTSGEKPSTSGEKPSTSGEQPSTSGEQPSTSGETTIYFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>F6PWT9</accession>
    <accession>A0A1W2W7M5</accession>
    <name>F6PWT9_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized LOC100178897</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC100178897</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01000196">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002126012.2">
        <property type="nucleotide sequence ID" value="XM_002125976.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6PWT9"/>
    <dbReference type="Ensembl" id="ENSCINT00000025281.2">
        <property type="protein sequence ID" value="ENSCINP00000025035.2"/>
        <property type="gene ID" value="ENSCING00000013687.2"/>
    </dbReference>
    <dbReference type="GeneID" id="100178897"/>
    <dbReference type="KEGG" id="cin:100178897"/>
    <dbReference type="HOGENOM" id="CLU_1854490_0_0_1"/>
    <dbReference type="InParanoid" id="F6PWT9"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000025035.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="138" mass="15026" checksum="0D5DF1530A486C7F" modified="2012-04-18" version="2">MNTISPSPPNNYQLRPAICSALIFLAVVFGGVVCMVVNSTNTKPRLEYLLLGVTCLGIGTIATSVSIICFVLKQSRVIEKIPPNKAPPPKSVPFVVDRLMARAATNLEYRACEDLLSSNTVRPSTTMHCAYKSNYHVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>F6QIK7</accession>
    <name>F6QIK7_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Translation factor GUF1 homolog, mitochondrial</fullName>
            <ecNumber evidence="9">3.6.5.n1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Elongation factor 4 homolog</fullName>
            <shortName evidence="9">EF-4</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">GTPase GUF1 homolog</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Ribosomal back-translocase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Promotes mitochondrial protein synthesis. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Binds to mitochondrial ribosomes in a GTP-dependent manner.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.n1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Mitochondrion inner membrane</location>
            <topology evidence="9">Peripheral membrane protein</topology>
            <orientation evidence="9">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the GTP-binding elongation factor family. LepA subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. LepA subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.n1" evidence="9"/>
    <dbReference type="AlphaFoldDB" id="F6QIK7"/>
    <dbReference type="FunCoup" id="F6QIK7">
        <property type="interactions" value="89"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000006440"/>
    <dbReference type="Ensembl" id="ENSCINT00000006440.3">
        <property type="protein sequence ID" value="ENSCINP00000006440.3"/>
        <property type="gene ID" value="ENSCING00000003163.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00550000074940"/>
    <dbReference type="HOGENOM" id="CLU_009995_3_3_1"/>
    <dbReference type="InParanoid" id="F6QIK7"/>
    <dbReference type="OMA" id="QVKCDEN"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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                <person name="Terry A."/>
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                <person name="Tassy O."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Ueno K."/>
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                <person name="Matsumoto J."/>
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                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
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            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1">wild type</strain>
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    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01000514">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002125278.1">
        <property type="nucleotide sequence ID" value="XM_002125242.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6RDE0"/>
    <dbReference type="STRING" id="7719.ENSCINP00000025493"/>
    <dbReference type="Ensembl" id="ENSCINT00000025739.2">
        <property type="protein sequence ID" value="ENSCINP00000025493.2"/>
        <property type="gene ID" value="ENSCING00000014003.2"/>
    </dbReference>
    <dbReference type="GeneID" id="100176061"/>
    <dbReference type="KEGG" id="cin:100176061"/>
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    <dbReference type="OMA" id="KHHFTHA"/>
    <dbReference type="OrthoDB" id="6154001at2759"/>
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        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="266" mass="30891" checksum="E5228B1121B6AB18" modified="2012-04-18" version="2">MDFQDAEKTNCSSIDVIKSVSKKWKFYFEIRRREDGFDPSPSYIPRRLFPNLLPRPMLMQAPQDVSLKHHFTHAQVRCALANIQSYQPHVASPRTSARGEKINRNIKRMLKQMHVEHPMWHANQHDDKRITRQHSLRKVGVRGKGSEKALKKSMPVLLRASPPYLNDTEKQRLLDSTCEIILGTERIGKPTESYMEIGNTKLQSHNNPTPLQYNFTVPIHRRYVEAPPRIIKDSTLIHKGRSVYVYDQHGRRNKVVVKKDPISNTA</sequence>
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    <accession>F6RJS7</accession>
    <name>F6RJS7_CIOIN</name>
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            <fullName evidence="3">Attractin/MKLN-like beta-propeller domain-containing protein</fullName>
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    <organism evidence="4 5">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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    <reference evidence="5" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">wild type</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="STRING" id="7719.ENSCINP00000001190"/>
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        <property type="protein sequence ID" value="ENSCINP00000001190.3"/>
        <property type="gene ID" value="ENSCING00000000650.3"/>
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        <property type="component" value="Chromosome 12"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Kelch-type beta propeller"/>
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        <property type="entry name" value="Kelch-typ_b-propeller"/>
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        <property type="entry name" value="Kelch_1"/>
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        <property type="entry name" value="KELCH-LIKE PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632">
        <property type="entry name" value="LD33804P"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="6"/>
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        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
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    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Attractin/MKLN-like beta-propeller" evidence="3">
        <location>
            <begin position="102"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF24981"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="394" mass="43887" checksum="4BB54D30BDC23A61" modified="2012-04-18" version="2">MFNSILKWSTKDTKERKESTLKLLKCLHYAKIPQSSLTNYVLAQDIVMQDKELLTIIEGLIGEDKKKILQRLKKQMSTTAPPSDKNGGIGDGNSPLHNYVIVTGGRLYDQFTAQIQVLNHKDQTWKELTRLPSTLCHHSVAVVGNVLYVAGGEDTAIAGVKGLSSLWAFYPMKNEWETLPDMIQGRAHFCMSAQEQCLVVVGGKSSNVIRNSVEKFSILTKKWEFAAVLKQPRYSHAGSSYKHDLYVSGGVVRKSSTQDMLKYNIATNHWVAVAKMKCARSCHTMAAVRDRIYVFGGENETQGVIQHVSTAIECYTPQCDQWHKVAQKTISQCDKESACTELDGLVYLVGGCKTNVTDHRIDTASYNPESDTWATQPKLPRRLRGAACAVLALK</sequence>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
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                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
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                <person name="Inaba K."/>
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                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
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                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
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                <person name="Richardson P."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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    </dbReference>
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        <property type="entry name" value="Glutathione gamma-glutamylcysteinyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.30">
        <property type="entry name" value="Phytochelatin synthase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040409">
        <property type="entry name" value="PCS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007719">
        <property type="entry name" value="PCS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038156">
        <property type="entry name" value="PCS_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33447">
        <property type="entry name" value="GLUTATHIONE GAMMA-GLUTAMYLCYSTEINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33447:SF2">
        <property type="entry name" value="GLUTATHIONE GAMMA-GLUTAMYLCYSTEINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05023">
        <property type="entry name" value="Phytochelatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51443">
        <property type="entry name" value="PCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0104">Cadmium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Peptidase C83" evidence="5">
        <location>
            <begin position="2"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000022610.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="333" mass="37812" checksum="DD53FE2928FB8898" modified="2012-04-18" version="2">MATKTNFYRRILPEICINFSSDDGKLIFKEALDNGGMEIFFHLGPQIRTQDEPAYCGLSSLVIVLNGLAVDPGRVWKGVWRWYHESMLECCTSLEDVQRQGVTIEQLACIAKCNQLNADVVYVDDHASIDDFRELVKQSCRGSSMAVICSFGRRELGQTGSGHFSPIGGYHPGKDVVLVLEPARFKYPPYWLSLEALWNAMNTKDPTNGDSPRGYMILEKSNSQPTLLFRISRYFNAINPLHEDAADFLQKAVKFLKEKPEKPNNSHQVIKDVAENLLKISAEHKLNHEKGILFNTFKKSQLESMSPEHQTAMFSLVEEIESLELYQYLRDAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>F6SSX2</accession>
    <name>F6SSX2_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Cytosol aminopeptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100176888</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">wild type</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M17 family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002609">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6SSX2"/>
    <dbReference type="FunCoup" id="F6SSX2">
        <property type="interactions" value="47"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000016401"/>
    <dbReference type="Ensembl" id="ENSCINT00000016401.3">
        <property type="protein sequence ID" value="ENSCINP00000016401.3"/>
        <property type="gene ID" value="ENSCING00000008012.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063255"/>
    <dbReference type="HOGENOM" id="CLU_013734_1_2_1"/>
    <dbReference type="InParanoid" id="F6SSX2"/>
    <dbReference type="OMA" id="WPMPLPE"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00433">
        <property type="entry name" value="Peptidase_M17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.220.10">
        <property type="entry name" value="Leucine Aminopeptidase, subunit E, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011356">
        <property type="entry name" value="Leucine_aapep/pepB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043472">
        <property type="entry name" value="Macro_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000819">
        <property type="entry name" value="Peptidase_M17_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11963:SF23">
        <property type="entry name" value="CYTOSOL AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11963">
        <property type="entry name" value="LEUCINE AMINOPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00883">
        <property type="entry name" value="Peptidase_M17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00481">
        <property type="entry name" value="LAMNOPPTDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52949">
        <property type="entry name" value="Macro domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00631">
        <property type="entry name" value="CYTOSOL_AP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytosol aminopeptidase" evidence="5">
        <location>
            <begin position="372"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS00631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000016401.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="524" mass="56915" checksum="073C034E7A46996D" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>F6SYR3</accession>
    <accession>A0A1W2W3H4</accession>
    <name>F6SYR3_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">Carbonic anhydrase</fullName>
            <ecNumber evidence="2 6">4.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100179118</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Reversible hydration of carbon dioxide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>hydrogencarbonate + H(+) = CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:10748"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="EC" id="4.2.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the alpha-carbonic anhydrase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.1" evidence="2 6"/>
    <dbReference type="AlphaFoldDB" id="F6SYR3"/>
    <dbReference type="STRING" id="7719.ENSCINP00000026304"/>
    <dbReference type="Ensembl" id="ENSCINT00000026550.2">
        <property type="protein sequence ID" value="ENSCINP00000026304.2"/>
        <property type="gene ID" value="ENSCING00000014586.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000167899"/>
    <dbReference type="HOGENOM" id="CLU_039326_1_2_1"/>
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        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
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        <location>
            <begin position="23"/>
            <end position="299"/>
        </location>
    </feature>
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        <location>
            <begin position="22"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010718"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00012925"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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            <dbReference type="PROSITE" id="PS51144"/>
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            <dbReference type="Ensembl" id="ENSCINP00000026304.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="299" mass="32617" checksum="F790FBA598159F6A" modified="2012-04-18" version="2">MKAYLVVAILLVASLDNTTGNAAWDYGAQGPSTWQNNYTTCGSRSQSPIDIVDSAVTSQAKSVPFVTSSLSAPTSMSIHNNGHSLQVDMTGGYTITNAAFLPNSYKAVQYHFHWGNTTGSEHKLKGQQFFGEMHIVHYNTKYPNISQALNQPQGLAVLAFFIKDTASKSNVNFAKITDQIQNGFASNTNKNIPAFAPSLLLPKLSVYYRYNGSLTTPPCSETVIWTVFKEPIEISASQATILKTKVYKTGTTPVFDNFRPTQPLNGRKILKYQISGAASYKAQGFVATFLMAALVCILN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>F6SYS0</accession>
    <name>F6SYS0_CIOIN</name>
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        <recommendedName>
            <fullName evidence="11">G-protein coupled receptors family 3 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
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    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 3 family. GABA-B receptor subfamily.</text>
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    <dbReference type="AlphaFoldDB" id="F6SYS0"/>
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    <dbReference type="Ensembl" id="ENSCINT00000026549.2">
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        <property type="gene ID" value="ENSCING00000014585.2"/>
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        <property type="entry name" value="GPCR_3_C"/>
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        <property type="entry name" value="GABA-B RECEPTOR"/>
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        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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        <location>
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            <end position="53"/>
        </location>
    </feature>
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        <location>
            <begin position="73"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="11">
        <location>
            <begin position="13"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00008991"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF00003"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000026303.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="102" mass="11650" checksum="47870EE3547F3D26" modified="2012-04-18" version="2">MYLSPCLVGSSAFIRDIPTTEDVITWKTCCFAGICCFDVGFTLMFGSLAAKTWRIDKIFQQNSRKQVVTDEKLLCAVFLLVCLEIVILIMWHIHDQSVCYFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>F6TEQ5</accession>
    <name>F6TEQ5_CIOIN</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
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    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
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            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
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                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">wild type</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
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</entry>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
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                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
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                <person name="DeSantis R."/>
                <person name="Doyle S."/>
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                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
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                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
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    </reference>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
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                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
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                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">wild type</strain>
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    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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            <topology evidence="1">Multi-pass membrane protein</topology>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="8">wild type</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Putnam N."/>
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                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
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                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
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                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">wild type</strain>
        </source>
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    <reference evidence="7" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="F6VU73"/>
    <dbReference type="STRING" id="7719.ENSCINP00000024302"/>
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    <dbReference type="Pfam" id="PF20826">
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    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="226"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="193"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="206"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
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    <evidence type="ECO:0000256" key="5">
        <source>
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            <dbReference type="PROSITE" id="PS50280"/>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000024302.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="453" mass="51284" checksum="D66FFDCE83249A25" modified="2012-04-18" version="2">MAVGSKIETLPTVPVPCQQSPACNKHRGFGLPYQDHNYGALPPPPTPPLSPTTGFTANSLNGLDKLNEDRVVNSTTAFSNGLAAAGSENSTVLSDITRCICGFTHDDGYMICCDQCSVWQHIDCMSIDRNNIPETFLCDHCNPRSLNREEAIKLQTRKREDFLMGTDSSGTDSDVEMNNKVLYSSVQSTPTSLTITSKKQEAVSTGKKRKRSRENSKRNGKKNHFPSRKRSRSKSSCNETEECGISFSWARKLREWNEKYQEINENMYSQELLQAVLSSSSKMAVKEQSNLSKLREQLVKVWCQCLRFIPYIVQSTVQQLRNTVLTLEDVCMSHPVAEVCGSYMLSNQFEGVNSTLYERPYPFVFFYNGFDGLEICVDSRTTGNISRYIRRSCCPNCEIRHVMEDGKIHLFIYAKSRILNGAEITIPFDIKYQNCHYNMECGCSQFKDCPVYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>F6W122</accession>
    <name>F6W122_CIOIN</name>
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        <submittedName>
            <fullName evidence="20">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">wild type</strain>
        </source>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
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            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Synaptic cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="EAAA01002238">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000018782"/>
    <dbReference type="Ensembl" id="ENSCINT00000018782.3">
        <property type="protein sequence ID" value="ENSCINP00000018782.3"/>
        <property type="gene ID" value="ENSCING00000009244.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155527"/>
    <dbReference type="HOGENOM" id="CLU_010754_0_0_1"/>
    <dbReference type="InParanoid" id="F6W122"/>
    <dbReference type="OMA" id="MENAAWV"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097060">
        <property type="term" value="C:synaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="entry name" value="Gal_Rha_Lectin_LPHNs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.740:FF:000017">
        <property type="entry name" value="adhesion G protein-coupled receptor L3"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="GPCR, family 2, extracellular hormone receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR057244">
        <property type="entry name" value="GAIN_B"/>
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    <dbReference type="InterPro" id="IPR046338">
        <property type="entry name" value="GAIN_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
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    <dbReference type="InterPro" id="IPR036445">
        <property type="entry name" value="GPCR_2_extracell_dom_sf"/>
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    <dbReference type="InterPro" id="IPR001879">
        <property type="entry name" value="GPCR_2_extracellular_dom"/>
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    <dbReference type="InterPro" id="IPR003924">
        <property type="entry name" value="GPCR_2_latrophilin"/>
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    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
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    <dbReference type="InterPro" id="IPR000203">
        <property type="entry name" value="GPS"/>
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    <dbReference type="InterPro" id="IPR000922">
        <property type="entry name" value="Lectin_gal-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043159">
        <property type="entry name" value="Lectin_gal-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003112">
        <property type="entry name" value="Olfac-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050605">
        <property type="entry name" value="Olfactomedin-like_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23192:SF88">
        <property type="entry name" value="ADHESION G PROTEIN-COUPLED RECEPTOR L2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="OLFACTOMEDIN-RELATED"/>
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    <dbReference type="Pfam" id="PF00002">
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF02140">
        <property type="entry name" value="SUEL_Lectin"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="LATROPHILIN"/>
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    <dbReference type="SMART" id="SM00303">
        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00284">
        <property type="entry name" value="OLF"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
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    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="OLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50228">
        <property type="entry name" value="SUEL_LECTIN"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
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    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
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    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
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        <location>
            <begin position="764"/>
            <end position="792"/>
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    <feature type="transmembrane region" description="Helical" evidence="14">
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        <source>
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        <source>
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        <source>
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    </evidence>
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        <source>
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    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    </evidence>
    <sequence length="1029" mass="113907" checksum="3CFF8261561C87DC" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>F6W7I3</accession>
    <accession>A0A1W2WIK2</accession>
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        <recommendedName>
            <fullName evidence="3 10">T-complex protein 1 subunit gamma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100181285</name>
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    <organism evidence="11 12">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <reaction evidence="8">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
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            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the TCP-1 chaperonin family.</text>
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        <property type="project" value="GO_Central"/>
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        <property type="entry name" value="T-complex protein 1 subunit gamma"/>
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        <property type="entry name" value="T-complex protein 1 subunit gamma"/>
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        <property type="match status" value="1"/>
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    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
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        <source>
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        </source>
    </evidence>
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        <source>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU004191"/>
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    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000002814.3"/>
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    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="543" mass="59817" checksum="82728B5C9518676F" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>F6W8X6</accession>
    <name>F6W8X6_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Renin receptor N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="entry name" value="RENIN RECEPTOR"/>
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    <feature type="domain" description="Renin receptor N-terminal" evidence="1">
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            <begin position="25"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF25294"/>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    </evidence>
    <sequence length="248" mass="27744" checksum="554BB355B782FDAB" modified="2012-04-18" version="2">MKTYTFSHYGIIAWIFCLGLISTINGQLYIVSKPDSIQISSEGNNINGYDVPQIINYMMGFSSDMSTQLQGLIKYDIFDSPSANLLINIDLPTDVSFALNDFAVKYDKVSMEDDRVTSMEKLNKAITSLPWKEQSVIVDLVTDSGNTRNPIFNGLAFTNDEMMHRLALDDSLVKTFPSCLNPTNVIDQSFYAEIQLMQDILDKIKAHPEVCVDGTPDSYNFVLTSLENLKQVYGVDSCQVRSAISILD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>F6WD96</accession>
    <name>F6WD96_CIOIN</name>
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        <recommendedName>
            <fullName evidence="10">Fringe-like glycosyltransferase domain-containing protein</fullName>
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    </protein>
    <organism evidence="11 12">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Tunicata</taxon>
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            <taxon>Phlebobranchia</taxon>
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            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">wild type</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Endomembrane system</location>
            <topology evidence="9">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 31 family.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="ENSCINP00000017755.3"/>
        <property type="gene ID" value="ENSCING00000008704.3"/>
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        <property type="component" value="Chromosome 9"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="P:regulation of Notch signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Fringe"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003378">
        <property type="entry name" value="Fringe-like_glycosylTrfase"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Fringe-like glycosyltransferase" evidence="10">
        <location>
            <begin position="35"/>
            <end position="281"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
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<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
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            <taxon>Ciona</taxon>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="F6WI10"/>
    <dbReference type="STRING" id="7719.ENSCINP00000000018"/>
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        <property type="protein sequence ID" value="ENSCINP00000000018.3"/>
        <property type="gene ID" value="ENSCING00000000013.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160436"/>
    <dbReference type="HOGENOM" id="CLU_021316_5_0_1"/>
    <dbReference type="InParanoid" id="F6WI10"/>
    <dbReference type="OMA" id="MENFISH"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45913">
        <property type="entry name" value="EPM2A-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45913:SF19">
        <property type="entry name" value="LOW QUALITY PROTEIN: ZINC FINGER BED DOMAIN-CONTAINING PROTEIN 5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000000018.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="533" mass="61079" checksum="5AA31756A2A06FDD" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>F6X0N8</accession>
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        <recommendedName>
            <fullName evidence="5 14">Signal peptidase complex catalytic subunit SEC11</fullName>
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    <gene>
        <name evidence="16" type="primary">LOC100183780</name>
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    <organism evidence="16 17">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Catalytic component of the signal peptidase complex (SPC) which catalyzes the cleavage of N-terminal signal sequences from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum. Specifically cleaves N-terminal signal peptides that contain a hydrophobic alpha-helix (h-region) shorter than 18-20 amino acids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 14">
            <text>Cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins.</text>
            <dbReference type="EC" id="3.4.21.89"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="14">Component of the signal peptidase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the peptidase S26B family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.89" evidence="4 14"/>
    <dbReference type="AlphaFoldDB" id="F6X0N8"/>
    <dbReference type="FunCoup" id="F6X0N8">
        <property type="interactions" value="425"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000029455"/>
    <dbReference type="MEROPS" id="S26.010"/>
    <dbReference type="Ensembl" id="ENSCINT00000029701.2">
        <property type="protein sequence ID" value="ENSCINP00000029455.2"/>
        <property type="gene ID" value="ENSCING00000020486.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015600"/>
    <dbReference type="HOGENOM" id="CLU_089996_0_0_1"/>
    <dbReference type="InParanoid" id="F6X0N8"/>
    <dbReference type="OMA" id="ILMNEYP"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005787">
        <property type="term" value="C:signal peptidase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009003">
        <property type="term" value="F:signal peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06530">
        <property type="entry name" value="S26_SPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.109.10:FF:000003">
        <property type="entry name" value="Signal peptidase complex catalytic subunit SEC11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.109.10">
        <property type="entry name" value="Umud Fragment, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036286">
        <property type="entry name" value="LexA/Signal_pep-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019758">
        <property type="entry name" value="Pept_S26A_signal_pept_1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019756">
        <property type="entry name" value="Pept_S26A_signal_pept_1_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015927">
        <property type="entry name" value="Peptidase_S24_S26A/B/C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019533">
        <property type="entry name" value="Peptidase_S26"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001733">
        <property type="entry name" value="Peptidase_S26B"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02228">
        <property type="entry name" value="sigpep_I_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10806">
        <property type="entry name" value="SIGNAL PEPTIDASE COMPLEX CATALYTIC SUBUNIT SEC11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10806:SF6">
        <property type="entry name" value="SIGNAL PEPTIDASE COMPLEX CATALYTIC SUBUNIT SEC11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00717">
        <property type="entry name" value="Peptidase_S24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00728">
        <property type="entry name" value="SIGNALPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51306">
        <property type="entry name" value="LexA/Signal peptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00501">
        <property type="entry name" value="SPASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00761">
        <property type="entry name" value="SPASE_I_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 14" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 14" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="7 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="18"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S24/S26A/S26B/S26C" evidence="15">
        <location>
            <begin position="37"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011035"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00045533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU362047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000029455.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="179" mass="20453" checksum="04EB36B499501F31" modified="2012-04-18" version="2">NIKMEMFDDFKRMNKRQVYYQVLNFGMIIASALMIWKGMMVATGSESPIVVVLSGSMEPAFYRGDLLFLGNDRSDPIRAGEIVVFKIEGRDIPIVHRVIKVHETHEGKIKFLTKGDNNEVDDRGLYKPGQLWVEKHHVVGRARGFVPYVGMVTIIMNDYPKLKYAVLGLLGLFVLLHRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>F6X1E1</accession>
    <name>F6X1E1_CIOIN</name>
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        <recommendedName>
            <fullName evidence="13">tRNA (guanine(10)-N(2))-methyltransferase TRMT11</fullName>
            <ecNumber evidence="12">2.1.1.214</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">tRNA methyltransferase 11 homolog</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">wild type</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Catalytic subunit of the TRMT11-TRM112 methyltransferase complex, that specifically mediates the S-adenosyl-L-methionine-dependent N(2)-methylation of guanosine nucleotide at position 10 (m2G10) in tRNAs. This is one of the major tRNA (guanine-N(2))-methyltransferases.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>guanosine(10) in tRNA + S-adenosyl-L-methionine = N(2)-methylguanosine(10) in tRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43128"/>
            <dbReference type="Rhea" id="RHEA-COMP:10355"/>
            <dbReference type="Rhea" id="RHEA-COMP:10357"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74481"/>
            <dbReference type="EC" id="2.1.1.214"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:43129"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Part of the heterodimeric TRMT11-TRM112 methyltransferase complex; this complex forms an active tRNA methyltransferase, where TRMT112 acts as an activator of the catalytic subunit TRMT11.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM11 methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.214" evidence="12"/>
    <dbReference type="EMBL" id="EAAA01000609">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6X1E1"/>
    <dbReference type="FunCoup" id="F6X1E1">
        <property type="interactions" value="78"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000029438"/>
    <dbReference type="Ensembl" id="ENSCINT00000029684.2">
        <property type="protein sequence ID" value="ENSCINP00000029438.2"/>
        <property type="gene ID" value="ENSCING00000017363.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000018131"/>
    <dbReference type="HOGENOM" id="CLU_029646_0_0_1"/>
    <dbReference type="InParanoid" id="F6X1E1"/>
    <dbReference type="OMA" id="AFNKWSR"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043527">
        <property type="term" value="C:tRNA methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160102">
        <property type="term" value="F:tRNA (guanine(10)-N2)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000241">
        <property type="entry name" value="RlmKL-like_Mtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016691">
        <property type="entry name" value="TRMT11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059073">
        <property type="entry name" value="TRMT11_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13370">
        <property type="entry name" value="RNA METHYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13370:SF3">
        <property type="entry name" value="TRNA (GUANINE(10)-N2)-METHYLTRANSFERASE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25904">
        <property type="entry name" value="Tmrp11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01170">
        <property type="entry name" value="UPF0020"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017259">
        <property type="entry name" value="tRNA_mtfrase_TRM11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00507">
        <property type="entry name" value="N12N6MTFRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51627">
        <property type="entry name" value="SAM_MT_TRM11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 15" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 15" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 15" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 15" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="3 15" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="tRNA (guanine(10)-N(2))-methyltransferase TRMT11 N-terminal" evidence="17">
        <location>
            <begin position="9"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosomal RNA large subunit methyltransferase K/L-like methyltransferase" evidence="16">
        <location>
            <begin position="200"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00050985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00056270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00065434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00066937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00067484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00075308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF25904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000029438.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="456" mass="51462" checksum="E8D254EFD10C0C90" modified="2012-04-18" version="2">QCLDGFKCKYLILLAQDKLLAHISELESVAQTFGFKLNIKQKAKISPTTGKTDFTKWIILQEIAEENVLKLMSRSVCAMSAFELWGSGSDFSLLKQSILEYPLEQKRDCFKETQSFAIRVKGIGKKMSSEIQRERIDLLEDVLPFQGKVDLVNPSGEFYLVEDYSNRVQGQPPKEPDAILFGRLLCNGQRSLIERYSLKKRKFIGNTSMDVMLSILMTNLGGINKNSVVCDPFVGTGSLLIPAAHFGGYVIGGDINYNIIHARGRSSRKGAGWRQKDETIRHTLMEYGLRSQYLDVLLCDAACHAWNTNGKELFDAIITDPPYGIREACQKVGSKKQNPVVPELEPGLTHFPEQTEYHLEDVFKDLLNFAAQSLVAGGRLVYWLPVYRADYSPKVIPTHSLLKLMYNHEQILNSHSSRRLIVMEKLTKENVTETAQAVISSSVFSGHNSFRDKYFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>F6XSE6</accession>
    <name>F6XSE6_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">G-protein coupled receptors family 3 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="F6XSE6"/>
    <dbReference type="FunCoup" id="F6XSE6">
        <property type="interactions" value="4"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000005140"/>
    <dbReference type="Ensembl" id="ENSCINT00000005140.3">
        <property type="protein sequence ID" value="ENSCINP00000005140.3"/>
        <property type="gene ID" value="ENSCING00000002510.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234595"/>
    <dbReference type="HOGENOM" id="CLU_005389_0_0_1"/>
    <dbReference type="InParanoid" id="F6XSE6"/>
    <dbReference type="OMA" id="NEMACNQ"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001641">
        <property type="term" value="F:group II metabotropic glutamate receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007216">
        <property type="term" value="P:G protein-coupled glutamate receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051966">
        <property type="term" value="P:regulation of synaptic transmission, glutamatergic"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000302">
        <property type="entry name" value="Si:ch211-203b20.7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.30">
        <property type="entry name" value="GPCR, family 3, nine cysteines domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000337">
        <property type="entry name" value="GPCR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038550">
        <property type="entry name" value="GPCR_3_9-Cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050726">
        <property type="entry name" value="mGluR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24060">
        <property type="entry name" value="METABOTROPIC GLUTAMATE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01176">
        <property type="entry name" value="GABABRECEPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00248">
        <property type="entry name" value="GPCRMGR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50259">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
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                <person name="Yamada L."/>
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                <person name="Awazu S."/>
                <person name="Azumi K."/>
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                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
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                <person name="Richardson P."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
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                <person name="Wiley G.B."/>
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                <person name="Roe B.A."/>
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                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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    <reference evidence="9" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">wild type</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="3"/>
    <dbReference type="EMBL" id="EAAA01001389">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6XVY9"/>
    <dbReference type="FunCoup" id="F6XVY9">
        <property type="interactions" value="319"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000015365"/>
    <dbReference type="Ensembl" id="ENSCINT00000015365.3">
        <property type="protein sequence ID" value="ENSCINP00000015365.3"/>
        <property type="gene ID" value="ENSCING00000007494.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155050"/>
    <dbReference type="HOGENOM" id="CLU_002173_5_0_1"/>
    <dbReference type="InParanoid" id="F6XVY9"/>
    <dbReference type="OMA" id="GDCTNEF"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000058">
        <property type="term" value="P:regulation of ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00078">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2160.10:FF:000004">
        <property type="entry name" value="probable E3 ubiquitin-protein ligase HERC4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2410.10:FF:000003">
        <property type="entry name" value="probable E3 ubiquitin-protein ligase HERC4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.130.10">
        <property type="entry name" value="Ubiquitin-protein ligase E3A, N-terminal zinc-binding domain (AZUL)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032353">
        <property type="entry name" value="AZUL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042556">
        <property type="entry name" value="AZUL_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044611">
        <property type="entry name" value="E3A/B/C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45700:SF8">
        <property type="entry name" value="HECT-TYPE E3 UBIQUITIN TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45700">
        <property type="entry name" value="UBIQUITIN-PROTEIN LIGASE E3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16558">
        <property type="entry name" value="AZUL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="HECT" evidence="8">
        <location>
            <begin position="534"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="7">
        <location>
            <position position="830"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000015365.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="862" mass="97825" checksum="69544EB8CF11EFC3" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>F6Y516</accession>
    <name>F6Y516_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Calponin-homology (CH) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">wild type</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the calponin family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002033">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6Y516"/>
    <dbReference type="FunCoup" id="F6Y516">
        <property type="interactions" value="29"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000019941"/>
    <dbReference type="Ensembl" id="ENSCINT00000019941.3">
        <property type="protein sequence ID" value="ENSCINP00000019941.3"/>
        <property type="gene ID" value="ENSCING00000008778.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166959"/>
    <dbReference type="HOGENOM" id="CLU_055232_1_0_1"/>
    <dbReference type="InParanoid" id="F6Y516"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031032">
        <property type="term" value="P:actomyosin structure organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050606">
        <property type="entry name" value="Calponin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001997">
        <property type="entry name" value="Calponin/LIMCH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000557">
        <property type="entry name" value="Calponin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003096">
        <property type="entry name" value="SM22_calponin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47385">
        <property type="entry name" value="CALPONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47385:SF18">
        <property type="entry name" value="CALPONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00402">
        <property type="entry name" value="Calponin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00889">
        <property type="entry name" value="CALPONIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00888">
        <property type="entry name" value="SM22CALPONIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01052">
        <property type="entry name" value="CALPONIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51122">
        <property type="entry name" value="CALPONIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="6">
        <location>
            <begin position="50"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000019941.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="207" mass="22456" checksum="5B20B4BD2E986B09" modified="2012-04-18" version="2">STSTMANRPKAYGNSAEVASKIDAKYSQEDEMEIVAWISSLVQSSPEGKGKEAMKQNKEMENISVFLKAAAKYANIPAQDMFQTVDLYEGGNLGQVQSTIFKVGAEAKNRGLHGIGVKKAEENKRVFDEQKMREGRNIIGLQMGTNQGASQSGMSAYGLGRQMTQKKAGICKPNPTYTGSMQMGTNQVASQRGMTAYGLGRQLTPQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>F6YB51</accession>
    <name>F6YB51_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">tRNA (32-2'-O)-methyltransferase regulator THADA</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">wild type</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Together with methyltransferase FTSJ1, methylates the 2'-O-ribose of nucleotides at position 32 of the anticodon loop of substrate tRNAs.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the THADA family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001848">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6YB51"/>
    <dbReference type="STRING" id="7719.ENSCINP00000026135"/>
    <dbReference type="Ensembl" id="ENSCINT00000026381.2">
        <property type="protein sequence ID" value="ENSCINP00000026135.2"/>
        <property type="gene ID" value="ENSCING00000014447.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015500"/>
    <dbReference type="HOGENOM" id="CLU_745033_0_0_1"/>
    <dbReference type="InParanoid" id="F6YB51"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056842">
        <property type="entry name" value="THADA-like_TPR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019442">
        <property type="entry name" value="THADA/TRM732_DUF2428"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051954">
        <property type="entry name" value="tRNA_methyltransferase_THADA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14387">
        <property type="entry name" value="THADA/DEATH RECEPTOR INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14387:SF7">
        <property type="entry name" value="THYROID ADENOMA-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10350">
        <property type="entry name" value="DUF2428"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25151">
        <property type="entry name" value="TPR_Trm732_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003345835" description="tRNA (32-2'-O)-methyltransferase regulator THADA" evidence="5">
        <location>
            <begin position="23"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="domain" description="DUF2428" evidence="6">
        <location>
            <begin position="3"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA (32-2'-O)-methyltransferase regulator THADA-like C-terminal TPR repeats region" evidence="7">
        <location>
            <begin position="102"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00035625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF10350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF25151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000026135.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="372" mass="41932" checksum="1E4D7C4FE0D890B6" modified="2012-04-18" version="2">MICVTRRSAGLPFLLQALLGSAKFPQGQECLNYIVKSLFKLVESITLNGSDVAVPSDSTIHALNILRGLVKDASLGDEMLPYLSTCLKVAIVGFTSNNWQIRNASTLLFSSIIIRVFGVMKNFESSDKNRLSSYEFFTRMPELHQFFLQRLEEITKSDDLPKHTGLYPLLLVLSRLYPTATRTNSRLNKYIPLIVRCRQSPIYKCRVMAANALLSVLPQHEYRKVISQLFISLKKAVISRNSLHGTLIQLKALISSKNCSNTFPNICSQLICEEITEQIKSSKCMVVYASYIDLIIDVFLLNNPLSEDFQPRVKETLLCFIIDCLQYLKQTCTLTPGSTMFYTSFAKLLVYAQLSGIFTDGIKTCLQSNVLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>F6YGV4</accession>
    <name>F6YGV4_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="F6YGV4"/>
    <dbReference type="Ensembl" id="ENSCINT00000028145.2">
        <property type="protein sequence ID" value="ENSCINP00000027899.2"/>
        <property type="gene ID" value="ENSCING00000021798.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01140000283753"/>
    <dbReference type="HOGENOM" id="CLU_1664633_0_0_1"/>
    <dbReference type="InParanoid" id="F6YGV4"/>
    <dbReference type="OMA" id="LLWEFPV"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000027899.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="159" mass="18527" checksum="9FA119EC55E97F9C" modified="2012-04-18" version="2">NYLFLGKFPRLLLGKFPKLLFWEIPALLLWEFPVLLLWKFPGLLFGEIPGLLLRKFPRLLLRVFPVLLLREFPELLFGKIPGLLLRKFPGLLLWEFPVLLLREFPGLLFGEIPGLLLRKFPGLLLWEFPVLLLREFPGLLFGEIPGLLLRKFPGLLLGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>F6YTG4</accession>
    <name>F6YTG4_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized LOC100183517</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100183517</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="F6YTG4"/>
    <dbReference type="Ensembl" id="ENSCINT00000021846.2">
        <property type="protein sequence ID" value="ENSCINP00000021600.2"/>
        <property type="gene ID" value="ENSCING00000011256.2"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_180895_2_0_1"/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
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                <person name="De Tomaso A."/>
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                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
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                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="9" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <location evidence="1">Mitochondrion matrix</location>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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        <scope>IDENTIFICATION</scope>
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        <scope>IDENTIFICATION</scope>
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    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13723">
        <property type="entry name" value="ADAMTS A DISINTEGRIN AND METALLOPROTEASE WITH THROMBOSPONDIN MOTIFS PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13723:SF313">
        <property type="entry name" value="PEPTIDASE M12B DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19236">
        <property type="entry name" value="ADAMTS_CR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19030">
        <property type="entry name" value="TSP1_ADAMTS"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00090">
        <property type="entry name" value="TSP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01857">
        <property type="entry name" value="ADAMTSFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5047275484" description="Ig-like domain-containing protein" evidence="5">
        <location>
            <begin position="25"/>
            <end position="1746"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="861"/>
            <end position="943"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="1151"/>
            <end position="1255"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="1258"/>
            <end position="1359"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="1379"/>
            <end position="1471"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="43"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="47"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="58"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR613273-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000018668.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="1746" mass="194975" checksum="FCA40F5B1BBC6950" modified="2025-10-08" version="3"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>F6Z6E4</accession>
    <name>F6Z6E4_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Vesicle transport through interaction with t-SNAREs homolog 1A</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Vesicle transport v-SNARE protein Vti1-like 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Vti1-rp2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="11">Interacts with distinct SNARE complexes that contain either STX5 or STX6. Interacts with NAPA and, to a lesser extent, with NAPG. Identified in a complex containing STX6, STX12, VAMP4 and VTI1A.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Endomembrane system</location>
            <topology evidence="10">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VTI1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="F6Z6E4"/>
    <dbReference type="FunCoup" id="F6Z6E4">
        <property type="interactions" value="153"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000028512"/>
    <dbReference type="Ensembl" id="ENSCINT00000028758.2">
        <property type="protein sequence ID" value="ENSCINP00000028512.2"/>
        <property type="gene ID" value="ENSCING00000016458.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183192"/>
    <dbReference type="HOGENOM" id="CLU_075474_1_0_1"/>
    <dbReference type="InParanoid" id="F6Z6E4"/>
    <dbReference type="OMA" id="MEYEAND"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012507">
        <property type="term" value="C:ER to Golgi transport vesicle membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006896">
        <property type="term" value="P:Golgi to vacuole transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016236">
        <property type="term" value="P:macroautophagy"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048280">
        <property type="term" value="P:vesicle fusion with Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000078">
        <property type="entry name" value="Vesicle transport through interaction with t-SNAREs 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.400:FF:000001">
        <property type="entry name" value="Vesicle transport through interaction with t-SNAREs homolog 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.400">
        <property type="entry name" value="t-snare proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010989">
        <property type="entry name" value="SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038407">
        <property type="entry name" value="v-SNARE_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007705">
        <property type="entry name" value="Vesicle_trsprt_v-SNARE_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21230:SF26">
        <property type="entry name" value="VESICLE TRANSPORT THROUGH INTERACTION WITH T-SNARES HOMOLOG 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21230">
        <property type="entry name" value="VESICLE TRANSPORT V-SNARE PROTEIN VTI1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05008">
        <property type="entry name" value="V-SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12352">
        <property type="entry name" value="V-SNARE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47661">
        <property type="entry name" value="t-snare proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Vesicle transport v-SNARE N-terminal" evidence="16">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="34"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00046280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00065755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00071612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00081711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00082368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF05008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000028512.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="176" mass="20484" checksum="BF014D556C536C4D" modified="2012-04-18" version="2">MASLLLSYEQQFSTLTAEVTQRMGTIHNYHGTERKSLISSIERQLDEAQELLEQMDLEIRELPNSERGKLMGRMKSYQTEFNNLETQMKKAKTTNNANKLRDELLSMDDNGTDDQRARLLDNTERLENSSRRLEVGYRVVMETEKVGQDILDNLQRDRETISRTRQRLRDGDANLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F6ZJ24</accession>
    <name>F6ZJ24_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
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                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
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        <citation type="submission" date="2025-09" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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    <accession>F6ZQ58</accession>
    <accession>A0A1W3JJQ9</accession>
    <name>F6ZQ58_CIOIN</name>
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        <name evidence="16" type="primary">LOC104266855</name>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
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                <person name="Miura S."/>
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                <person name="Nomoto H."/>
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                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
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                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="16">wild type</strain>
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    <reference evidence="16" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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    <comment type="function">
        <text evidence="9">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III (Pol III) which synthesizes small non-coding RNAs including 5S rRNA, snRNAs, tRNAs and miRNAs from at least 500 distinct genomic loci. With CRCP/RPC9 forms a mobile stalk that protrudes from Pol III core and functions primarily in transcription initiation. Pol III plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF-kappa-B through the RIG-I pathway.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the RNA polymerase III complex consisting of 17 subunits: a ten-subunit horseshoe-shaped catalytic core composed of POLR3A/RPC1, POLR3B/RPC2, POLR1C/RPAC1, POLR1D/RPAC2, POLR3K/RPC10, POLR2E/RPABC1, POLR2F/RPABC2, POLR2H/RPABC3, POLR2K/RPABC4 and POLR2L/RPABC5; a mobile stalk composed of two subunits POLR3H/RPC8 and CRCP/RPC9, protruding from the core and functioning primarily in transcription initiation; and additional subunits homologous to general transcription factors of the RNA polymerase II machinery, POLR3C/RPC3-POLR3F/RPC6-POLR3G/RPC7 heterotrimer required for transcription initiation and POLR3D/RPC4-POLR3E/RPC5 heterodimer involved in both transcription initiation and termination. Interacts with CRCP/RPC9. POLR3H/RPC8 and CRCP/RPC9 probably form a Pol III subcomplex.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic RPB7/RPC8 RNA polymerase subunit family.</text>
    </comment>
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        </source>
    </evidence>
    <sequence length="205" mass="23199" checksum="FE28A81D2EDB6BF1" modified="2011-07-27" version="1">MFILVEMKDTVKVEPWLFNVNLNDAVKVELNRRLANKVVYNIGVCICLFDILKYEDSYIFPGDGASHTKVHFRCVVFRPFVDEVLIGKIKGCSQEGVTVSLGFFDDILIPPESLQQPSKFDESEQVWVWEYETDEGTHDLFMDVNEPIRLRVVDETFTDTTPMSAPPTTATTSTEVASKKPTIPYLIKAVISEPGLGLLCWWTNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>F6ZSG1</accession>
    <name>F6ZSG1_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">B(0,+)-type amino acid transporter 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100186978</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="F6ZSG1"/>
    <dbReference type="Ensembl" id="ENSCINT00000016227.3">
        <property type="protein sequence ID" value="ENSCINP00000016227.3"/>
        <property type="gene ID" value="ENSCING00000015156.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162520"/>
    <dbReference type="HOGENOM" id="CLU_007946_3_0_1"/>
    <dbReference type="InParanoid" id="F6ZSG1"/>
    <dbReference type="OMA" id="GIFISPH"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003333">
        <property type="term" value="P:amino acid transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000036">
        <property type="entry name" value="Solute carrier family 7 member 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050598">
        <property type="entry name" value="AminoAcid_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11785">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11785:SF512">
        <property type="entry name" value="SOBREMESA, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="45"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="134"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="162"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="243"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="284"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="340"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="365"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="396"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="423"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000016227.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="473" mass="51436" checksum="D5B016920F3C7D13" modified="2012-04-18" version="2">GRGKRFTTKRSIGLYSGVFVTAGVIIGSGIFISPHGVLAAANYSVGITLIIWVLGGIVAMLSTLCYCELATSIPESGGDYTYLTYAYHPALAFLIPWMYTCVPSSDAALVLTFARYATEPFFVGSKPPEESVKLVAICLMLLLTAVNILSVKCALRMQVAFAASKFLAIGVVVVGGIIYVIRDNSVAKQNFTSSFNSQALKGFSVLSLSGAFYQVMWSYDGWNSLCHVTEEVKNPGKTIPKASILSIAFVMFAYLVINCAYFSVLSVDEMGSSKIVALPFALKAMGGAAWIVPLTVCLCTAGSYNGGVLTTGRVSYVAARRGHLPQLFGMLHIYKHTPSAALILNAIGCIVLVSIGQLGPLIDTFGFVNWTFKGLSALSVLILRHRMPDLQRPYKVPTVIPVFLTLLSAFFVILPLINDPHFLYFYALAFCALGLVLYFTFVHKRIRVPGLENATKFLQQFLLVAPSEWRKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>F7A410</accession>
    <accession>A0A1W2W552</accession>
    <name>F7A410_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">UPF0472 protein C16orf72 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100181256</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_002124318.1">
        <property type="nucleotide sequence ID" value="XM_002124282.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F7A410"/>
    <dbReference type="FunCoup" id="F7A410">
        <property type="interactions" value="68"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000015537"/>
    <dbReference type="Ensembl" id="ENSCINT00000015537.3">
        <property type="protein sequence ID" value="ENSCINP00000015537.3"/>
        <property type="gene ID" value="ENSCING00000007586.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100181256"/>
    <dbReference type="KEGG" id="cin:100181256"/>
    <dbReference type="GeneTree" id="ENSGT00390000002886"/>
    <dbReference type="HOGENOM" id="CLU_081329_1_0_1"/>
    <dbReference type="InParanoid" id="F7A410"/>
    <dbReference type="OMA" id="KDVIAWV"/>
    <dbReference type="OrthoDB" id="5823474at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040308">
        <property type="entry name" value="HAPR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029196">
        <property type="entry name" value="HAPSTR1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31624:SF4">
        <property type="entry name" value="CHROMOSOME 16 OPEN READING FRAME 72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31624">
        <property type="entry name" value="UPF0472 PROTEIN C16ORF72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15251">
        <property type="entry name" value="TAPR1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="142"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="191"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="198"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000015537.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="254" mass="28860" checksum="C74898C6AD49726B" modified="2011-07-27" version="1">MEEGKNNKKDFTGDRELQDHSPGKWFSNWEEQCLLQAEVEAVDEAALTSERRTLEQKLWLSFQTTASAIAQLYKDRAQSSDDLWVSFQSAAEFATNMYKDSVEGCKNMTEVAAQRGYHRRNKEVLSWAKKKRRHIRREDLIGYLCGRSPPHRGSRPSSSHRPQEANRDSRLQTFREAVSMNGLNGAMAGISFGGDSPLRQTSSRNTQTFHPDLPAFLSEEFQRRNDLLAPSSPSRKRASASGHDSKPSSPKRRK</sequence>
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                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">wild type</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Essential component of the ubiquitin-dependent proteolytic pathway which degrades ubiquitin fusion proteins. The ternary complex containing UFD1, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope. It may be involved in the development of some ectoderm-derived structures. Acts as a negative regulator of type I interferon production via the complex formed with VCP and NPLOC4, which binds to RIGI and recruits RNF125 to promote ubiquitination and degradation of RIGI.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein degradation; proteasomal ubiquitin-dependent pathway.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UFD1 family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001384">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F7AP04"/>
    <dbReference type="FunCoup" id="F7AP04">
        <property type="interactions" value="963"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000015288"/>
    <dbReference type="Ensembl" id="ENSCINT00000015288.3">
        <property type="protein sequence ID" value="ENSCINP00000015288.3"/>
        <property type="gene ID" value="ENSCING00000007440.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000002408"/>
    <dbReference type="HOGENOM" id="CLU_037790_2_0_1"/>
    <dbReference type="InParanoid" id="F7AP04"/>
    <dbReference type="OMA" id="WMMQQLC"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036501">
        <property type="term" value="C:UFD1-NPL4 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034098">
        <property type="term" value="C:VCP-NPL4-UFD1 AAA ATPase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031593">
        <property type="term" value="F:polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030970">
        <property type="term" value="P:retrograde protein transport, ER to cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.50:FF:000001">
        <property type="entry name" value="Ubiquitin fusion degradation protein 1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.330.10:FF:000002">
        <property type="entry name" value="ubiquitin fusion degradation protein 1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.50">
        <property type="entry name" value="Ubiquitin fusion degradation protein UFD1, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004854">
        <property type="entry name" value="Ufd1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042299">
        <property type="entry name" value="Ufd1-like_Nn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055417">
        <property type="entry name" value="UFD1_N1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055418">
        <property type="entry name" value="UFD1_N2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12555">
        <property type="entry name" value="UBIQUITIN FUSION DEGRADATON PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12555:SF13">
        <property type="entry name" value="UBIQUITIN RECOGNITION FACTOR IN ER-ASSOCIATED DEGRADATION PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03152">
        <property type="entry name" value="UFD1_N1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24842">
        <property type="entry name" value="UFD1_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Ubiquitin fusion degradation protein UFD1 N-terminal subdomain 1" evidence="6">
        <location>
            <begin position="19"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin fusion degradation protein UFD1 N-terminal subdomain 2" evidence="7">
        <location>
            <begin position="121"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00058252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00060618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00072593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF24842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000015288.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="313" mass="35395" checksum="BED8E33C1F58A986" modified="2012-04-18" version="2">MLQDFLNEFNMGARRPNTFSNGYRCYSSSFGALSDQKSKDIQKGGKIIMPPSALDQLSRLNISYPMLFKLTNSPKGRSTHCGVLEFVAEEGVIYLPYWMMQNLLLGEGDLVQLENCTLPVATYARFQPQSTDFHDISNPKAVLENALRNFACLTKSDMIAIQYNSRQYELCVQEVRPQNAVCIIECDISLEFDAPVGYEPPVPKPKQVESDMIVGTPDMKKAIEEYMKETSGFSAFEGTGNRLDGKKKRTRQKKVEIDVSNIERGVPNYKWKFGQLTFIRNNEPKKQTADTDTSNDFAVFSGTGQKIRKKKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>F7AUP3</accession>
    <name>F7AUP3_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glutathione S-transferase omega</fullName>
            <shortName evidence="5">GSTO</shortName>
            <ecNumber evidence="5">1.20.4.2</ecNumber>
            <ecNumber evidence="5">1.8.5.1</ecNumber>
            <ecNumber evidence="5">2.5.1.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Glutathione-dependent dehydroascorbate reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Monomethylarsonic acid reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100176187</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Exhibits glutathione-dependent thiol transferase activity. Has high dehydroascorbate reductase activity and may contribute to the recycling of ascorbic acid. Participates in the biotransformation of inorganic arsenic and reduces monomethylarsonic acid (MMA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 5">
            <text>L-dehydroascorbate + 2 glutathione = glutathione disulfide + L-ascorbate</text>
            <dbReference type="Rhea" id="RHEA:24424"/>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:58297"/>
            <dbReference type="ChEBI" id="CHEBI:58539"/>
            <dbReference type="EC" id="1.8.5.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 5">
            <text>methylarsonate + 2 glutathione + H(+) = methylarsonous acid + glutathione disulfide + H2O</text>
            <dbReference type="Rhea" id="RHEA:15969"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17826"/>
            <dbReference type="ChEBI" id="CHEBI:33409"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:58297"/>
            <dbReference type="EC" id="1.20.4.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the GST superfamily. Omega family.</text>
    </comment>
    <dbReference type="EC" id="1.20.4.2" evidence="5"/>
    <dbReference type="EC" id="1.8.5.1" evidence="5"/>
    <dbReference type="EC" id="2.5.1.18" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="F7AUP3"/>
    <dbReference type="STRING" id="7719.ENSCINP00000011385"/>
    <dbReference type="Ensembl" id="ENSCINT00000011385.3">
        <property type="protein sequence ID" value="ENSCINP00000011385.3"/>
        <property type="gene ID" value="ENSCING00000005510.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163896"/>
    <dbReference type="HOGENOM" id="CLU_011226_9_2_1"/>
    <dbReference type="InParanoid" id="F7AUP3"/>
    <dbReference type="OMA" id="VAWMRTA"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045174">
        <property type="term" value="F:glutathione dehydrogenase (ascorbate) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050610">
        <property type="term" value="F:methylarsonate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000009">
        <property type="entry name" value="Glutathione S-transferase omega-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000123">
        <property type="entry name" value="Glutathione transferase o1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005442">
        <property type="entry name" value="GST_omega"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050983">
        <property type="entry name" value="GST_Omega/HSP26"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43968">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43968:SF6">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE OMEGA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13410">
        <property type="entry name" value="GST_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13417">
        <property type="entry name" value="GST_N_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01625">
        <property type="entry name" value="GSTRNSFRASEO"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <sequence length="244" mass="27219" checksum="3742D3253E4F060A" modified="2012-04-18" version="2">MGNSVHLKEGSPEPAAVAKGVLRVYGMKFCPYVHRLKLVLAAKEVAHETININLKSKPKWYFAKNPQGKVPTIEIDGNVIYESDITSEYVDAVYPGRKLSTTDALKAANEKMLLVYWGNKSVTNFYKYCMAKPDDKEAKDAALATLKEGLEYLATFLKKNGSVFICGSQPGLTDYFLYPHLERINAIVPAVDLKQIPKVKTYFDQMQEDKAVKSCRFSDELHREFGRKAVAGDIGAYDIGPVAQ</sequence>
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    <accession>F7AVW9</accession>
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            <fullName evidence="8">Kinesin-like protein</fullName>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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    <reference evidence="12" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">wild type</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
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        <text evidence="7 8">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
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    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="ENSCING00000007535.3"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="GO_Central"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="P-loop_NTPase"/>
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    </dbReference>
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        <property type="entry name" value="KINESINHEAVY"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="KINESIN_MOTOR_2"/>
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    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0493">Microtubule</keyword>
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            <end position="155"/>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
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    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000023041.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="575" mass="66192" checksum="FA9167115961F12A" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>F7AX97</accession>
    <name>F7AX97_CIOIN</name>
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        <recommendedName>
            <fullName evidence="9">Mitogen-activated protein kinase kinase kinase kinase</fullName>
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        </recommendedName>
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        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
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            <taxon>Metazoa</taxon>
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            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
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            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">wild type</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Serine/threonine kinase that plays a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="9"/>
    <dbReference type="EMBL" id="EAAA01002976">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F7AX97"/>
    <dbReference type="FunCoup" id="F7AX97">
        <property type="interactions" value="197"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000019505"/>
    <dbReference type="Ensembl" id="ENSCINT00000019505.3">
        <property type="protein sequence ID" value="ENSCINP00000019505.3"/>
        <property type="gene ID" value="ENSCING00000009594.3"/>
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    <dbReference type="GeneTree" id="ENSGT00940000155483"/>
    <dbReference type="HOGENOM" id="CLU_006347_1_0_1"/>
    <dbReference type="InParanoid" id="F7AX97"/>
    <dbReference type="OMA" id="SECKHAN"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0008349">
        <property type="term" value="F:MAP kinase kinase kinase kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
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    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="CDD" id="cd06613">
        <property type="entry name" value="STKc_MAP4K3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000031">
        <property type="entry name" value="Mitogen-activated protein kinase kinase kinase kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001180">
        <property type="entry name" value="CNH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021160">
        <property type="entry name" value="MAPKKKK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050629">
        <property type="entry name" value="STE20/SPS1-PAK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012:SF18">
        <property type="entry name" value="HAPPYHOUR, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012">
        <property type="entry name" value="STERILE20-LIKE KINASE, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00780">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038172">
        <property type="entry name" value="MAPKKKK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00036">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50219">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 9 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="16"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="CNH" evidence="15">
        <location>
            <begin position="556"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="319"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="390"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="512"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10">
        <location>
            <position position="136"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="22"/>
            <end position="30"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 12">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR038172"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR038172-1"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR038172-2"/>
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    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50011"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50219"/>
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    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000019505.3"/>
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    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>F7B1U4</accession>
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        <recommendedName>
            <fullName evidence="4">Protein FAM184A/B N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">wild type</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01001994">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="FunCoup" id="F7B1U4">
        <property type="interactions" value="12"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSCINT00000024998.2">
        <property type="protein sequence ID" value="ENSCINP00000024752.2"/>
        <property type="gene ID" value="ENSCING00000013501.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063669"/>
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    <dbReference type="InParanoid" id="F7B1U4"/>
    <dbReference type="OMA" id="MCKKVAQ"/>
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        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039478">
        <property type="entry name" value="FAM184A/B_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18870">
        <property type="entry name" value="PROTEIN TAG-278-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18870:SF9">
        <property type="entry name" value="PROTEIN TAG-278-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15665">
        <property type="entry name" value="FAM184"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein FAM184A/B N-terminal" evidence="4">
        <location>
            <begin position="23"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1040"/>
            <end position="1096"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="131"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="257"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="369"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="503"/>
            <end position="912"/>
        </location>
    </feature>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Putnam N."/>
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                <person name="Satake M."/>
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                <person name="Wang H.G."/>
                <person name="Awazu S."/>
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                <person name="Tokuoka M."/>
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                <person name="Zhang C."/>
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                <person name="Detter C."/>
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                <person name="Richardson P."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
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                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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            <authorList>
                <consortium name="Ensembl"/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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    </evidence>
    <sequence length="136" mass="15156" checksum="49539101B71BD301" modified="2011-07-27" version="1">MRFPKSVSNMGDIKEFQPKYIGSEKDGKFLNNNQNNNLHSGNGEELSHLFAYPAQSNFSGRKYPLGWIRSVRNGLLGNVLKVGGSWYTMLDAAAFVTSSKLDLKEYPADFVSMSFYKMFGFPTGIGALLVRNTSAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>F7BKF4</accession>
    <accession>A0A1W3JUB8</accession>
    <name>F7BKF4_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">tlx</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_009861844.1">
        <property type="nucleotide sequence ID" value="XM_009863542.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F7BKF4"/>
    <dbReference type="STRING" id="7719.ENSCINP00000012758"/>
    <dbReference type="Ensembl" id="ENSCINT00000012758.3">
        <property type="protein sequence ID" value="ENSCINP00000012758.3"/>
        <property type="gene ID" value="ENSCING00000006177.3"/>
    </dbReference>
    <dbReference type="GeneID" id="778781"/>
    <dbReference type="CTD" id="778781"/>
    <dbReference type="GeneTree" id="ENSGT00940000160629"/>
    <dbReference type="HOGENOM" id="CLU_573577_0_0_1"/>
    <dbReference type="InParanoid" id="F7BKF4"/>
    <dbReference type="OMA" id="YNGCRFT"/>
    <dbReference type="OrthoDB" id="9451579at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22249">
        <property type="entry name" value="UDM1_RNF168_RNF169-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000040">
        <property type="entry name" value="T-cell leukemia homeobox protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042247">
        <property type="entry name" value="TLX1/2/3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45921:SF6">
        <property type="entry name" value="C15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45921">
        <property type="entry name" value="IP01054P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3 6 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 6 7" id="KW-0371">Homeobox</keyword>
    <keyword evidence="5 6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="9">
        <location>
            <begin position="360"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="6">
        <location>
            <begin position="362"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="157"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="157"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000012758.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="476" mass="52548" checksum="6FF98178C50D96D0" modified="2011-07-27" version="1">MHQTRPMPAHIHGQENISFSINQILSPASEGSTMDDDNGVARDSYQYCGRSYEASSNTLNEKSNNNNSGSGNGYFYPQLGGYWGSDLYNGQYYNGCRFTDYYPNYGGRSNSIYGNEYPPNNHHAEPGRSTESCGKFGSNGIGSINNDKLSACSNATGTNVSGSDRNETLFRSNTSSDNSPTTKDPAQAQQPLSDPNPHDRHFSKFNNCRFRSCEVDGFNKLYDKESQCSQQQNTLGNHYSGAFAATNCYGSCCTGYAAAAALLTGGLPQTSCGVGSLATPALNVNPAHTTFTQQAAAMAAVDSAVAAATAGSGSLLRVPNHRSLHRSSLLTSNFPWMESRRERIALTRRIGHPYQNRTPPKKKKPRTSFSRIQICELEKRFHRQKYLASSERAALAKSLKMTDAQVKTWFQNRRTKWRRQTTEEREAERQAAQRLINQLQQEALARAPMPAPDAICLRNASLYALHNLQARLQNAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>F7BMV4</accession>
    <accession>A0A1W2WKA5</accession>
    <name>F7BMV4_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Centromere protein N-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100184308</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">wild type</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Centromere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CENP-N/CHL4 family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01000081">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002129120.1">
        <property type="nucleotide sequence ID" value="XM_002129084.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F7BMV4"/>
    <dbReference type="STRING" id="7719.ENSCINP00000008968"/>
    <dbReference type="Ensembl" id="ENSCINT00000008968.3">
        <property type="protein sequence ID" value="ENSCINP00000008968.3"/>
        <property type="gene ID" value="ENSCING00000004338.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100184308"/>
    <dbReference type="KEGG" id="cin:100184308"/>
    <dbReference type="GeneTree" id="ENSGT00390000004738"/>
    <dbReference type="HOGENOM" id="CLU_808815_0_0_1"/>
    <dbReference type="InParanoid" id="F7BMV4"/>
    <dbReference type="OMA" id="CRYEDYV"/>
    <dbReference type="OrthoDB" id="6585699at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000775">
        <property type="term" value="C:chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034080">
        <property type="term" value="P:CENP-A containing chromatin assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052011">
        <property type="entry name" value="CENP-NAC/CAD_complex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007902">
        <property type="entry name" value="Chl4/mis15/CENP-N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46790">
        <property type="entry name" value="CENTROMERE PROTEIN N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46790:SF1">
        <property type="entry name" value="CENTROMERE PROTEIN N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05238">
        <property type="entry name" value="CENP-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0137">Centromere</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004584"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00005566"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000008968.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="344" mass="39437" checksum="CC6ED03CA2D4EA18" modified="2012-04-18" version="2">MSDAKSAKLTLSSVLKRTNKAEMKMLLTSWGKLPLWKINFSQTKYELIQDICQICEEHDLSVKYANELDLLYVADFPHKKYWRAYKMTQPTGNVLTEEQCNANYIRNALEQKLYVSFSCSVSVMEKEHFLWGRVGIPDIKQVVYFIHFPTSKFLLFTKRNNKLKSLVQNALRRILGFKSIEDLPLSSPHPAALAQILLHSSDPHDSYEYDKGRYRPVQRLEEEHPLKKTDSRVNICDAGKLARNKRILDHQFGDKIQPCLTSLVLETETRFRGTKYLPTVPGNSIIKCKTTISGPNVLEGLRKLALLGYAKAPLPRFLTNISKTGRQCFKITDAARDDESRHSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>H2XJL2</accession>
    <name>H2XJL2_CIOIN</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">wild type</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01000656">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XJL2"/>
    <dbReference type="Ensembl" id="ENSCINT00000032968.1">
        <property type="protein sequence ID" value="ENSCINP00000029844.1"/>
        <property type="gene ID" value="ENSCING00000022433.1"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2290663_0_0_1"/>
    <dbReference type="InParanoid" id="H2XJL2"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000029844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="101" mass="11524" checksum="0DFC3DB52A859DB7" modified="2012-04-18" version="1">MRDVQVRNRVSNTHGLRMVDVTHHDEGASYFTSYRNSHDALGQSRGRGVPHVRKKPPVFNIISGEISAPTSGRIDHRLRSGNRVLSEQRRRSNIYQTFQLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>H2XJU0</accession>
    <name>H2XJU0_CIOIN</name>
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        <recommendedName>
            <fullName evidence="12">Ribosomal protein S6 kinase</fullName>
            <ecNumber evidence="12">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">wild type</strain>
        </source>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="12"/>
    <dbReference type="EMBL" id="EAAA01000762">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="FunCoup" id="H2XJU0">
        <property type="interactions" value="67"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000029922"/>
    <dbReference type="Ensembl" id="ENSCINT00000037212.1">
        <property type="protein sequence ID" value="ENSCINP00000029922.1"/>
        <property type="gene ID" value="ENSCING00000021826.1"/>
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    <dbReference type="HOGENOM" id="CLU_000288_58_0_1"/>
    <dbReference type="InParanoid" id="H2XJU0"/>
    <dbReference type="OMA" id="VEMIYAF"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
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    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0038202">
        <property type="term" value="P:TORC1 signaling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14092">
        <property type="entry name" value="STKc_MSK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05583">
        <property type="entry name" value="STKc_MSK_N"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Ribosomal protein S6 kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ribosomal protein S6 kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000686">
        <property type="entry name" value="Ribosomal protein S6 kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
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                <person name="Kobayashi K."/>
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                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
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                <person name="Nomoto H."/>
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                <person name="Sasaki A."/>
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                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
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                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
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                <person name="Levine M."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
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            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
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                <person name="Richardson P."/>
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    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
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                <person name="Chapman J."/>
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                <person name="De Tomaso A."/>
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                <person name="Goodstein D.M."/>
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                <person name="Hastings K.E."/>
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                <person name="Meinertzhagen I.A."/>
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                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
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                <person name="Mount S."/>
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                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
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                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
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                <person name="Zhang C."/>
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                <person name="Richardson P."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Ueno K."/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
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            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1">wild type</strain>
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        </citation>
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            <authorList>
                <consortium name="Ensembl"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
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                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
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                <person name="Yoshizaki F."/>
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                <person name="Zhang C."/>
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                <person name="Larimer F."/>
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                <person name="Doggett N."/>
                <person name="Glavina T."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    <evidence type="ECO:0000313" key="2">
        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
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                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
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                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
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                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <alternativeName>
            <fullName evidence="13">Neutrophil cytochrome b 22 kDa polypeptide</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Superoxide-generating NADPH oxidase light chain subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">p22 phagocyte B-cytochrome</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">p22-phox</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC100182370</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">wild type</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="14">Component of the phagocyte NADPH oxidase core complex/cytochrome b558 complex, composed of CYBB (heavy chain (beta)) and CYBA (light chain (alpha)). Component of the phagocyte NADPH oxidase complex composed of an obligatory core heterodimer formed by the membrane proteins CYBA and CYBB and the cytosolic regulatory subunits NCF1/p47-phox, NCF2/p67-phox, NCF4/p40-phox and the small GTPase RAC1 or RAC2. Interacts with NCF1 (via SH3 domain). Interacts with SH3PXD2A. Interacts with DUOX1, DUOX2 and TPO. Interacts with NOX4; this interaction mediates superoxide generation. Interacts with calprotectin (S100A8/9). Interacts with GBP7. Interacts with NOXO1. Forms a heterodimer with NOX3 and is essential for activity and cell membrane localization of NOX3. Interacts with NOX1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the p22phox family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001722">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002119312.1">
        <property type="nucleotide sequence ID" value="XM_002119276.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XNI4"/>
    <dbReference type="FunCoup" id="H2XNI4">
        <property type="interactions" value="33"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000031217"/>
    <dbReference type="Ensembl" id="ENSCINT00000036647.1">
        <property type="protein sequence ID" value="ENSCINP00000031217.1"/>
        <property type="gene ID" value="ENSCING00000022975.1"/>
    </dbReference>
    <dbReference type="GeneID" id="100182370"/>
    <dbReference type="KEGG" id="cin:100182370"/>
    <dbReference type="GeneTree" id="ENSGT00390000002290"/>
    <dbReference type="HOGENOM" id="CLU_125024_0_0_1"/>
    <dbReference type="InParanoid" id="H2XNI4"/>
    <dbReference type="OMA" id="ARTGQYC"/>
    <dbReference type="OrthoDB" id="2445232at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043020">
        <property type="term" value="C:NADPH oxidase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042554">
        <property type="term" value="P:superoxide anion generation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007732">
        <property type="entry name" value="Cyt_b558_asu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15168">
        <property type="entry name" value="CYTOCHROME B-245 LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15168:SF0">
        <property type="entry name" value="CYTOCHROME B-245 LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05038">
        <property type="entry name" value="Cytochrom_B558a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="46"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="98"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="120"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="146"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="150"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00033347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00050017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000031217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="175" mass="19091" checksum="6A67828F2EE7DD0A" modified="2012-04-18" version="1">MPSSSSNIRSIQWGMWANETALLGSYVLTLGGIIGIVGGLLKNFMFWLPIGIYGVVFGILVGLLEYPRGKKNKGNTLLRSGQSCFSTMVNKLPFVSSYYFRAIAYFIVCIPGIISVPTFLGSVCVIVGSGIYLGAALHKERWNPIESRPQVPSTSNDITQPPSQPPPRLPQNKQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>H2XNU9</accession>
    <name>H2XNU9_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CRAL-TRIO domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01001081">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XNU9"/>
    <dbReference type="FunCoup" id="H2XNU9">
        <property type="interactions" value="5"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000031332"/>
    <dbReference type="Ensembl" id="ENSCINT00000030796.1">
        <property type="protein sequence ID" value="ENSCINP00000031332.1"/>
        <property type="gene ID" value="ENSCING00000023909.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160026"/>
    <dbReference type="InParanoid" id="H2XNU9"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902936">
        <property type="term" value="F:phosphatidylinositol bisphosphate binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.20">
        <property type="entry name" value="N-terminal domain of phosphatidylinositol transfer protein sec14p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011074">
        <property type="entry name" value="CRAL/TRIO_N_dom"/>
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    <dbReference type="EMBL" id="EAAA01001840">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XS44"/>
    <dbReference type="STRING" id="7719.ENSCINP00000032478"/>
    <dbReference type="Ensembl" id="ENSCINT00000035241.1">
        <property type="protein sequence ID" value="ENSCINP00000032478.1"/>
        <property type="gene ID" value="ENSCING00000021124.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000167121"/>
    <dbReference type="HOGENOM" id="CLU_000660_7_0_1"/>
    <dbReference type="InParanoid" id="H2XS44"/>
    <dbReference type="OMA" id="NSACEAS"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00109">
        <property type="entry name" value="Kunitz-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000005">
        <property type="entry name" value="ADAM metallopeptidase with thrombospondin type 1 motif 9"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.410.10">
        <property type="entry name" value="Pancreatic trypsin inhibitor Kunitz domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013273">
        <property type="entry name" value="ADAMTS/ADAMTS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050439">
        <property type="entry name" value="ADAMTS_ADAMTS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002223">
        <property type="entry name" value="Kunitz_BPTI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036880">
        <property type="entry name" value="Kunitz_BPTI_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020901">
        <property type="entry name" value="Prtase_inh_Kunz-CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13723">
        <property type="entry name" value="ADAMTS A DISINTEGRIN AND METALLOPROTEASE WITH THROMBOSPONDIN MOTIFS PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13723:SF281">
        <property type="entry name" value="PAPILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00014">
        <property type="entry name" value="Kunitz_BPTI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19030">
        <property type="entry name" value="TSP1_ADAMTS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00090">
        <property type="entry name" value="TSP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01857">
        <property type="entry name" value="ADAMTSFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00759">
        <property type="entry name" value="BASICPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00131">
        <property type="entry name" value="KU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57362">
        <property type="entry name" value="BPTI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00280">
        <property type="entry name" value="BPTI_KUNITZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50279">
        <property type="entry name" value="BPTI_KUNITZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003578136" description="BPTI/Kunitz inhibitor domain-containing protein" evidence="7">
        <location>
            <begin position="24"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="domain" description="BPTI/Kunitz inhibitor" evidence="8">
        <location>
            <begin position="472"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="51"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="55"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="66"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR613273-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000032478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="523" mass="58264" checksum="31FB8333C844FA62" modified="2012-04-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>H2XSH7</accession>
    <name>H2XSH7_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Enoyl-CoA delta isomerase 1, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">3,2-trans-enoyl-CoA isomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17 18">Delta(3),Delta(2)-enoyl-CoA isomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Dodecenoyl-CoA isomerase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">wild type</strain>
        </source>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Key enzyme of fatty acid beta-oxidation. Able to isomerize both 3-cis (3Z) and 3-trans (3E) double bonds into the 2-trans (2E) form in a range of enoyl-CoA species, with a preference for (3Z)-enoyl-CoAs over (3E)-enoyl-CoAs. The catalytic efficiency of this enzyme is not affected by the fatty acyl chain length.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>(2E)-tetradecenoyl-CoA = (3Z)-tetradecenoyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:29847"/>
            <dbReference type="ChEBI" id="CHEBI:61405"/>
            <dbReference type="ChEBI" id="CHEBI:61968"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="12">
            <dbReference type="Rhea" id="RHEA:29849"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>(3Z)-decenoyl-CoA = (2E)-decenoyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:77195"/>
            <dbReference type="ChEBI" id="CHEBI:61406"/>
            <dbReference type="ChEBI" id="CHEBI:195601"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:77196"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>(3Z)-dodecenoyl-CoA = (2E)-dodecenoyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:23716"/>
            <dbReference type="ChEBI" id="CHEBI:57330"/>
            <dbReference type="ChEBI" id="CHEBI:58543"/>
            <dbReference type="EC" id="5.3.3.8"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:23717"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>(3Z)-hexenoyl-CoA = (2E)-hexenoyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:45748"/>
            <dbReference type="ChEBI" id="CHEBI:62077"/>
            <dbReference type="ChEBI" id="CHEBI:85415"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:45749"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>(3Z)-octenoyl-CoA = (2E)-octenoyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:46044"/>
            <dbReference type="ChEBI" id="CHEBI:62242"/>
            <dbReference type="ChEBI" id="CHEBI:85640"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:46045"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; fatty acid beta-oxidation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotrimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="EAAA01002798">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XSH7"/>
    <dbReference type="FunCoup" id="H2XSH7">
        <property type="interactions" value="84"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000032611"/>
    <dbReference type="Ensembl" id="ENSCINT00000032075.1">
        <property type="protein sequence ID" value="ENSCINP00000032611.1"/>
        <property type="gene ID" value="ENSCING00000023669.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000005678"/>
    <dbReference type="HOGENOM" id="CLU_009834_7_5_1"/>
    <dbReference type="InParanoid" id="H2XSH7"/>
    <dbReference type="OMA" id="ALCKCAE"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004165">
        <property type="term" value="F:delta(3)-delta(2)-enoyl-CoA isomerase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000034">
        <property type="entry name" value="Enoyl-CoA delta isomerase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11941:SF45">
        <property type="entry name" value="ENOYL-COA DELTA ISOMERASE 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11941">
        <property type="entry name" value="ENOYL-COA HYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
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                <person name="De Tomaso A."/>
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                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
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                <person name="Zhang C."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
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                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">wild type</strain>
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        <scope>IDENTIFICATION</scope>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="entry name" value="endopeptidase domain like (from Nostoc punctiforme)"/>
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        <property type="entry name" value="Papain-like_cys_pep_sf"/>
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        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_14, WHOLE GENOME SHOTGUN SEQUENCE"/>
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        <property type="entry name" value="NLPC_P60 DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
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        <property type="entry name" value="Cysteine proteinases"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="253" mass="29472" checksum="A9C3CE5A0CB99104" modified="2012-04-18" version="1">MAKNKEVSLQEAFLKFKRKKQEELNYGKRATQRSPLRHKFVEQCRSYIGVPYKKRYHVTGSAAYNSPLFLDCCGLIRRVLRDLRADFGFNIGPWNQGYMFDTLPLVVEDVAEMKAGDLVFVIGRHFKQGVKRPAHDVVHVEVWAGDCYKTISARWNNGFVQVFDDYRFESRSYGDMTYHFRSIDTWLEGVCKSFCPEHPWHRKEQKLSTRSIFYCDVIDGDVATSQHYDVIAKDLADRALCCALAELRLELYV</sequence>
</entry>
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    <accession>H2XUL2</accession>
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            <fullName evidence="10">Crk-like protein</fullName>
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    <organism evidence="8 9">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">wild type</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CRK family.</text>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="Ensembl" id="ENSCINT00000033904.1">
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        <property type="gene ID" value="ENSCING00000021667.1"/>
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    <dbReference type="GeneTree" id="ENSGT00820000127055"/>
    <dbReference type="InParanoid" id="H2XUL2"/>
    <dbReference type="OMA" id="SMTRQEA"/>
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        <property type="component" value="Chromosome 12"/>
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    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="F:receptor tyrosine kinase binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="F:signaling adaptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007167">
        <property type="term" value="P:enzyme-linked receptor protein signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902531">
        <property type="term" value="P:regulation of intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd09926">
        <property type="entry name" value="SH2_CRK_like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="adapter molecule crk isoform X1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="Tyrosine-phos_adapter"/>
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        <property type="match status" value="1"/>
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    <proteinExistence type="inferred from homology"/>
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        <location>
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        <location>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        <source>
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    </evidence>
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        <source>
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        </source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="299" mass="33972" checksum="50AA0B821F8847FE" modified="2012-04-18" version="1">MASANFNSADEDSWYFGMVSRKDSQNKLLHQKHGSFLVRDSTTCPGDYVLSVSENSKVSHYIINKQENRLKIGDQMFDSMPELLDFYKVHYLDTTTLIEPIPKPIKPQQTVNPIGSGMVNMSSITSPIKEPEVAKKREGLPMMVRALFDFKSDDVDDLPFSKHEILEVIEKPEENWWNARNAQGRIGQIPVPYVEPYVMNRNSTPGTYQMPGPKHGYFHPTPRPTSMPSRTGPVFAEVITRRVPNAYDPTALALEVGDRIQVTQMNKSGQWEGICNNKQGKFPFTHVKLIDFNDSESNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>H2XUU1</accession>
    <name>H2XUU1_CIOIN</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
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    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
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                <person name="Ogasawara M."/>
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                <person name="Ueno K."/>
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                <person name="Matsumoto J."/>
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                <person name="Wiley G.B."/>
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                <person name="Roe B.A."/>
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            <authorList>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
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                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
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                <person name="Wiley G.B."/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
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                <person name="Kawashima T."/>
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                <person name="Meinertzhagen I.A."/>
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                <person name="Wang H.G."/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Huang W."/>
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                <person name="Lemaire P."/>
                <person name="Martinez D."/>
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                <person name="Spagnuolo A."/>
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                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
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                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
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                <person name="Detter C."/>
                <person name="Doggett N."/>
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                <person name="Levine M."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
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                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
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                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
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                <person name="Wada S."/>
                <person name="Zhang C."/>
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                <person name="Larimer F."/>
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                <person name="Richardson P."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">wild type</strain>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Wang H.G."/>
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                <person name="Yoshizaki F."/>
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                <person name="Zhang C."/>
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                <person name="Doggett N."/>
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                <person name="Richardson P."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
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                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
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                <person name="Wiley G.B."/>
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                <person name="Roe B.A."/>
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                <person name="Hastings K.E."/>
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    <reference evidence="2" key="3">
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
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                <person name="Hayashi H."/>
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                <person name="Kano S."/>
                <person name="Kobayashi K."/>
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                <person name="Lee B.I."/>
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                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
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                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
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                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
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                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:003656">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
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    <dbReference type="InterPro" id="IPR003107">
        <property type="entry name" value="HAT"/>
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    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
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    <dbReference type="InterPro" id="IPR013949">
        <property type="entry name" value="Utp6"/>
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    <dbReference type="InterPro" id="IPR056907">
        <property type="entry name" value="UTP6_C"/>
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    <dbReference type="InterPro" id="IPR055347">
        <property type="entry name" value="UTP6_N"/>
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    <dbReference type="PANTHER" id="PTHR23271">
        <property type="entry name" value="HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGEN 66"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23271:SF1">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 6 HOMOLOG"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF08640">
        <property type="entry name" value="U3_assoc_6"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF24892">
        <property type="entry name" value="UTP6_C"/>
        <property type="match status" value="1"/>
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    <dbReference type="SMART" id="SM00386">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="U3 small nucleolar RNA-associated protein 6 N-terminal" evidence="6">
        <location>
            <begin position="10"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="U3 small nucleolar RNA-associated protein 6 homolog C-terminal" evidence="7">
        <location>
            <begin position="311"/>
            <end position="588"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010734"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF24892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000035636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="611" mass="71548" checksum="059D65A0E5B27F0F" modified="2012-04-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>H2Y169</accession>
    <name>H2Y169_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Septin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">wild type</strain>
        </source>
    </reference>
    <reference evidence="25 26" key="3">
        <citation type="journal article" date="2024" name="J. Mol. Biol." volume="436" first="168693" last="168693">
            <title>Structural Insights into Ciona intestinalis Septins: Complexes Suggest a Mechanism for Nucleotide-dependent Interfacial Cross-talk.</title>
            <authorList>
                <person name="Mendonca D.C."/>
                <person name="Morais S.T.B."/>
                <person name="Ciol H."/>
                <person name="Pinto A.P.A."/>
                <person name="Leonardo D.A."/>
                <person name="Pereira H.D."/>
                <person name="Valadares N.F."/>
                <person name="Portugal R.V."/>
                <person name="Klaholz B.P."/>
                <person name="Garratt R.C."/>
                <person name="Araujo A.P.U."/>
            </authorList>
            <dbReference type="PubMed" id="38960133"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2024.168693"/>
        </citation>
        <scope>STRUCTURE BY ELECTRON MICROSCOPY (2.72 ANGSTROMS) OF 14-415 IN COMPLEX WITH GDP</scope>
    </reference>
    <reference evidence="23" key="4">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="5">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Chromosome</location>
            <location evidence="5">Centromere</location>
            <location evidence="5">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cleavage furrow</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Cilium axoneme</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Midbody</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19 20">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01000686">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PDB" id="9BHT">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.26 A"/>
        <property type="chains" value="C/D=14-415"/>
    </dbReference>
    <dbReference type="PDB" id="9BHW">
        <property type="method" value="EM"/>
        <property type="resolution" value="2.72 A"/>
        <property type="chains" value="A/D=14-415"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2Y169"/>
    <dbReference type="EMDB" id="EMD-44552"/>
    <dbReference type="EMDB" id="EMD-44555"/>
    <dbReference type="SMR" id="H2Y169"/>
    <dbReference type="FunCoup" id="H2Y169">
        <property type="interactions" value="631"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000035653"/>
    <dbReference type="Ensembl" id="ENSCINT00000030861.1">
        <property type="protein sequence ID" value="ENSCINP00000035653.1"/>
        <property type="gene ID" value="ENSCING00000021379.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154222"/>
    <dbReference type="HOGENOM" id="CLU_017718_8_1_1"/>
    <dbReference type="InParanoid" id="H2Y169"/>
    <dbReference type="OMA" id="RSNPMGS"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032154">
        <property type="term" value="C:cleavage furrow"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0015630">
        <property type="term" value="C:microtubule cytoskeleton"/>
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        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0031105">
        <property type="term" value="C:septin complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0005940">
        <property type="term" value="C:septin ring"/>
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    <dbReference type="GO" id="GO:0005819">
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        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
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        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
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    <dbReference type="GO" id="GO:0061640">
        <property type="term" value="P:cytoskeleton-dependent cytokinesis"/>
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        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
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        <property type="project" value="GO_Central"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="G_SEPTIN_dom"/>
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        <property type="entry name" value="P-loop_NTPase"/>
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        <property type="entry name" value="Septin"/>
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    <dbReference type="InterPro" id="IPR008115">
        <property type="entry name" value="Septin7"/>
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    <dbReference type="PANTHER" id="PTHR18884">
        <property type="entry name" value="SEPTIN"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00735">
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Septin"/>
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        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="G_SEPTIN"/>
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    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="25 26" id="KW-0002">3D-structure</keyword>
    <keyword evidence="17" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0132">Cell division</keyword>
    <keyword evidence="16" id="KW-0966">Cell projection</keyword>
    <keyword evidence="18" id="KW-0137">Centromere</keyword>
    <keyword evidence="6" id="KW-0158">Chromosome</keyword>
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    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="14 20" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="11" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="10" id="KW-0498">Mitosis</keyword>
    <keyword evidence="9 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Septin-type G" evidence="22">
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            <begin position="39"/>
            <end position="308"/>
        </location>
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        <location>
            <begin position="307"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="408"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="307"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="408"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="21">
        <location>
            <begin position="419"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>GDP</name>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>GDP</name>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <label>2</label>
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    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>GDP</name>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <label>1</label>
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    <feature type="binding site" evidence="25">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>GDP</name>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
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    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="53"/>
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        <ligand>
            <name>GDP</name>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
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    <feature type="binding site" evidence="26">
        <location>
            <position position="54"/>
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        <ligand>
            <name>GDP</name>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
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    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="54"/>
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        <ligand>
            <name>GDP</name>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <label>1</label>
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        <ligand>
            <name>GDP</name>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <label>2</label>
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        <ligand>
            <name>GDP</name>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <label>1</label>
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        <ligand>
            <name>GDP</name>
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            <label>1</label>
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        <ligand>
            <name>GDP</name>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
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        <ligand>
            <name>GDP</name>
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        <ligand>
            <name>GDP</name>
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            <name>GDP</name>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <label>1</label>
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        <ligand>
            <name>GDP</name>
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            <name>GDP</name>
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            <name>GDP</name>
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        <ligand>
            <name>GDP</name>
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        <ligand>
            <name>GDP</name>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <label>1</label>
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        <ligand>
            <name>GDP</name>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <label>3</label>
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        <ligand>
            <name>GDP</name>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
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            <position position="195"/>
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        <ligand>
            <name>GDP</name>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <label>1</label>
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        <location>
            <position position="195"/>
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        <ligand>
            <name>GDP</name>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <label>3</label>
        </ligand>
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        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>GDP</name>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <label>3</label>
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        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>GDP</name>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <label>1</label>
        </ligand>
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    <feature type="binding site" evidence="26">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>GDP</name>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <label>2</label>
        </ligand>
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        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>GDP</name>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004626"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022776"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRNR" id="PIRNR006698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU004560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000035653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="25">
        <source>
            <dbReference type="PDB" id="9BHT"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="26">
        <source>
            <dbReference type="PDB" id="9BHW"/>
        </source>
    </evidence>
    <sequence length="428" mass="49740" checksum="E57CA97D6528C6B4" modified="2012-04-18" version="1">NIKIIKSLLFTIQQQPKALDSYVGFANLPNQLYRKSVKQGFEFTLMVVGESGLGKSTLINSLFLSDLYSNEYPGPSLRIKKTVKVETTKVLIKENGVTLRLTIDDTPGFGDAVDNSNCWQAVINHIEKKFEDYLNAESMVTRSKLADNRVHCCLYFIAPTGHGLKPLDVEFMKNLHDKVNIIPLIAKADTMTPEECLRFKKQIMKEIHEHKIQLYEFPECEDEEENRLNRKLKSRVPFAVVGSNTVLEIGGRRVRGRQYPWGVAEVENIDHCDFTVLRNMLVRTHMQDLKDVTNNVHYENYRSKKLSSVTTTTMDGRSKAQTKSPLAQMEEEKSEHMQKMKKMEMEMEEVFRMKVHEKKQKLQESEADLSRRHEAMKKKLEAEYAALDEKQRQYEKDKQDFEALQLKLMEDRKSDKSMDKKKKTKTLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>H2Y1D0</accession>
    <accession>A0A1W3JDN5</accession>
    <name>H2Y1D0_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">E3 ubiquitin protein ligase</fullName>
            <ecNumber evidence="6">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC104265921</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the BRE1 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="H2Y1D0"/>
    <dbReference type="STRING" id="7719.ENSCINP00000035714"/>
    <dbReference type="Ensembl" id="ENSCINT00000035880.1">
        <property type="protein sequence ID" value="ENSCINP00000035714.1"/>
        <property type="gene ID" value="ENSCING00000021449.1"/>
    </dbReference>
    <dbReference type="GeneID" id="104265921"/>
    <dbReference type="KEGG" id="cin:104265921"/>
    <dbReference type="GeneTree" id="ENSGT00390000002866"/>
    <dbReference type="HOGENOM" id="CLU_1323498_0_0_1"/>
    <dbReference type="InParanoid" id="H2Y1D0"/>
    <dbReference type="OrthoDB" id="10266039at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013956">
        <property type="entry name" value="E3_ubiquit_lig_Bre1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23163:SF0">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE BRE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23163">
        <property type="entry name" value="RING FINGER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="6 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="1"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU365038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000035714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="208" mass="24460" checksum="A6E61F0D411E53D1" modified="2012-04-18" version="1">MEKISKELKTTNAEMKELKLLLDMYKGLSKEQRDKAEIMANEKVATSARDDLEKKIRSLEEDVAREDARSKELEKKLQRSEEQLADAVVKMKEEKDRREKKEEELLLRGPDADSLRKMKVLEETIIELRRNLSATKQEEEALLSEMDVTGQAFEDMQEQNMRLLQQLREKDDANFKLMSERIKANQVHKLLQEEKSVLQDQNNTLHMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>H2Y1R7</accession>
    <name>H2Y1R7_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
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            <title>Systematic analysis of embryonic expression profiles of zinc finger genes in Ciona intestinalis.</title>
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            <title>Systematic analysis of embryonic expression profiles of zinc finger genes in Ciona intestinalis.</title>
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                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2003" name="Dev. Genes Evol." volume="213" first="211" last="212">
            <title>Genomewide surveys of developmentally relevant genes in Ciona intestinalis.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Satoh N."/>
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            <dbReference type="PubMed" id="12736827"/>
            <dbReference type="DOI" id="10.1007/s00427-003-0330-z"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="journal article" date="2004" name="Development" volume="131" first="4047" last="4058">
            <title>Gene expression profiles of transcription factors and signaling molecules in the ascidian embryo: towards a comprehensive understanding of gene networks.</title>
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                <person name="Imai K.S."/>
                <person name="Hino K."/>
                <person name="Yagi K."/>
                <person name="Satoh N."/>
                <person name="Satou Y."/>
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            <dbReference type="DOI" id="10.1242/dev.01270"/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2005-04" db="EMBL/GenBank/DDBJ databases">
            <title>Expressed genes in Ciona intestinalis.</title>
            <authorList>
                <person name="Satou Y."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="11" key="5">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="11">wild type</strain>
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    <reference evidence="11" key="6">
        <citation type="submission" date="2025-05" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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            <location evidence="1">Nucleus</location>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
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                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
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                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <citation type="journal article" date="2003" name="Dev. Genes Evol." volume="213" first="211" last="212">
            <title>Genomewide surveys of developmentally relevant genes in Ciona intestinalis.</title>
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        <citation type="journal article" date="2004" name="Development" volume="131" first="4047" last="4058">
            <title>Gene expression profiles of transcription factors and signaling molecules in the ascidian embryo: towards a comprehensive understanding of gene networks.</title>
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                <person name="Hino K."/>
                <person name="Yagi K."/>
                <person name="Satoh N."/>
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            <dbReference type="DOI" id="10.1242/dev.01270"/>
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        <citation type="submission" date="2005-04" db="EMBL/GenBank/DDBJ databases">
            <title>Expressed genes in Ciona intestinalis.</title>
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                <person name="Satou Y."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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    <reference evidence="16" key="5">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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</entry>
<entry dataset="TrEMBL" created="2004-12-07" modified="2026-01-28" version="133" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2003-10" db="EMBL/GenBank/DDBJ databases">
            <title>Molecular structure and evolution of EKN1 genes in a new combination of co-chaperone p23 with tetratricopeptide repeat domain.</title>
            <authorList>
                <person name="Chen Y."/>
                <person name="Dong W."/>
                <person name="Peng J."/>
                <person name="Huang C."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Neuron projection</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Dynein axonemal particle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AY428511">
        <property type="protein sequence ID" value="AAR89529.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001027805.1">
        <property type="nucleotide sequence ID" value="NM_001032633.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q5VJS0"/>
    <dbReference type="STRING" id="7719.ENSCINP00000032421"/>
    <dbReference type="Ensembl" id="ENSCINT00000030556.1">
        <property type="protein sequence ID" value="ENSCINP00000032421.1"/>
        <property type="gene ID" value="ENSCING00000023078.1"/>
    </dbReference>
    <dbReference type="GeneID" id="493858"/>
    <dbReference type="KEGG" id="cin:493858"/>
    <dbReference type="CTD" id="493858"/>
    <dbReference type="GeneTree" id="ENSGT00390000004930"/>
    <dbReference type="HOGENOM" id="CLU_029084_0_0_1"/>
    <dbReference type="OMA" id="ELAAWHF"/>
    <dbReference type="OrthoDB" id="348005at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120293">
        <property type="term" value="C:dynein axonemal particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003341">
        <property type="term" value="P:cilium movement"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036159">
        <property type="term" value="P:inner dynein arm assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:outer dynein arm assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="entry name" value="p23_DYX1C1_like"/>
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        <property type="entry name" value="dynein assembly factor 4, axonemal isoform X1"/>
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    </dbReference>
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        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="CS_dom"/>
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    <dbReference type="InterPro" id="IPR037894">
        <property type="entry name" value="CS_DYX1C1"/>
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    <dbReference type="InterPro" id="IPR052004">
        <property type="entry name" value="Dynein_assembly_factor_4"/>
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    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
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        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46492">
        <property type="entry name" value="DYNEIN ASSEMBLY FACTOR 4, AXONEMAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46492:SF1">
        <property type="entry name" value="DYNEIN AXONEMAL ASSEMBLY FACTOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04969">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
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    <keyword evidence="5 11" id="KW-0802">TPR repeat</keyword>
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        <location>
            <begin position="3"/>
            <end position="87"/>
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        <location>
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        <location>
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            <end position="191"/>
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
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    </evidence>
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    <accession>A0A1W2W4A3</accession>
    <accession>F7ANJ6</accession>
    <name>A0A1W2W4A3_CIOIN</name>
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        <name evidence="2" type="primary">LOC100178603</name>
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        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Phlebobranchia</taxon>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_002123548.4"/>
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        <location>
            <begin position="60"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000027276.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="248" mass="27761" checksum="7CDF3BCE93EA0406" modified="2017-06-07" version="1">MTSNKPETIPRMRHHHEDEAPIRHNTLLSPDMSSVELEPRQAFDDDVMKKHRNQFCKTRYYCVIGVAIVFLCFTIGFYSSIVDDSDAMTPRDIIDYDVDLTLDEEVTDDDVVDDVVDDADILAEAKSKFDAEDSKSFVIVVGDENVEVTLDQDGSIVLPDGKNLNPHDRNHWEVIQAVLGDAYYDYDVDQKDDNETDSNAALAADAGLKYVPPDVDEEHEHDDDCEQTISVNVADETGDSPSNETGNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>F6PHL6</accession>
    <name>F6PHL6_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">T-box transcription factor Ci-Tbx6b</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">tbx6b</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
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                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">wild type</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Required for ribosome biogenesis. Part of a complex which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Pseudouridine ("psi") residues may serve to stabilize the conformation of rRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the small nucleolar ribonucleoprotein particles containing H/ACA-type snoRNAs (H/ACA snoRNPs).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
            <location evidence="1 8">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 8">Belongs to the GAR1 family.</text>
    </comment>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_002130294.1"/>
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    </dbReference>
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        <property type="gene ID" value="ENSCING00000005744.3"/>
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        <property type="component" value="Chromosome 10"/>
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        <property type="entry name" value="Gar1/Naf1_Cbf5-bd_sf"/>
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        <property type="entry name" value="H/ACA_rnp_Gar1/Naf1"/>
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        <property type="entry name" value="Transl_B-barrel_sf"/>
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        <property type="entry name" value="NUCLEOLAR PROTEIN FAMILY A MEMBER 1 SNORNP PROTEIN GAR1"/>
        <property type="match status" value="1"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00038293"/>
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    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU364004"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000011858.3"/>
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    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="198" mass="20715" checksum="359256A34AE6754E" modified="2012-04-18" version="2">MSFRGNRGRGGGGFRGGGGFRGRGGGGGRGGRGRGGYGGGNRYNDEPPDYVVEIGKVMHPCQEDLVCKCTNEKIPYFNAPIYLENKSKIGKVDEIFGQIRDFCFSIKMSDNMLASSFSSDTKFFIDPAKLLPLQRFLPQPPGSIQKRGGRGGGRGGRGGRGGGRGGGRGGFRGRGGGGFRGSSRGGGGRGRGGRGRGW</sequence>
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    <accession>F6QA01</accession>
    <accession>A0A1W3JNH1</accession>
    <name>F6QA01_CIOIN</name>
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        <submittedName>
            <fullName evidence="2">G protein pathway suppressor 2</fullName>
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    </protein>
    <gene>
        <name evidence="2" type="primary">LOC100185651</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_009863130.3"/>
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        <property type="protein sequence ID" value="ENSCINP00000006171.3"/>
        <property type="gene ID" value="ENSCING00000003032.3"/>
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    <dbReference type="GeneID" id="100185651"/>
    <dbReference type="GeneTree" id="ENSGT00390000004049"/>
    <dbReference type="HOGENOM" id="CLU_860400_0_0_1"/>
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    <dbReference type="OMA" id="FWQFKRV"/>
    <dbReference type="OrthoDB" id="10038194at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
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    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0000318"/>
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    <dbReference type="InterPro" id="IPR026094">
        <property type="entry name" value="GPS2"/>
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        <property type="entry name" value="G PROTEIN PATHWAY SUPPRESSOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="G PROTEIN PATHWAY SUPPRESSOR 2"/>
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        <source>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000006171.3"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>F6QG58</accession>
    <name>F6QG58_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">rRNA methyltransferase 2, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA methyltransferase RlmE family.</text>
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        <property type="entry name" value="Vaccinia Virus protein VP39"/>
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        <property type="entry name" value="SAM-dependent_MTases_sf"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ribosomal RNA methyltransferase FtsJ" evidence="7">
        <location>
            <begin position="73"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00041184"/>
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    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01728"/>
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    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000025277.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="202" mass="22545" checksum="EABD696B037D500D" modified="2012-04-18" version="2">MTTLPKSICRLYNVVTQRIQHRSVHLGQAVEIKKRKIRVGSKNFMEIPIKGTIEYVSRQLSDPFVKMRHLEGYRCRSAYKLMDIVEKHDLLKPGMKVVDLGAAPGSWSQVAAERVINKDDDGLVIAVDLLKFPPLDGVTQITGDFTKKETQDEIKALLGTEENRFVDIVLCDAAPSASGFKNKDHLAIVQIIAAATNFTLKV</sequence>
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    <accession>F6QJE4</accession>
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        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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            <taxon>Eukaryota</taxon>
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    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">wild type</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01002505">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="F6QJE4"/>
    <dbReference type="STRING" id="7719.ENSCINP00000006420"/>
    <dbReference type="Ensembl" id="ENSCINT00000006420.3">
        <property type="protein sequence ID" value="ENSCINP00000006420.3"/>
        <property type="gene ID" value="ENSCING00000003153.3"/>
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    <dbReference type="HOGENOM" id="CLU_1444050_0_0_1"/>
    <dbReference type="InParanoid" id="F6QJE4"/>
    <dbReference type="OMA" id="KTIWTIS"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008381">
        <property type="term" value="F:mechanosensitive monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027272">
        <property type="entry name" value="Piezo"/>
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        <property type="entry name" value="Piezo_cap_dom"/>
    </dbReference>
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        <property type="entry name" value="Piezo_THU9_anchor"/>
    </dbReference>
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        <property type="entry name" value="PIEZO-TYPE MECHANOSENSITIVE ION CHANNEL HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47049:SF2">
        <property type="entry name" value="PIEZO-TYPE MECHANOSENSITIVE ION CHANNEL HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="Piezo THU9 and anchor" evidence="3">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="Piezo non-specific cation channel cap" evidence="2">
        <location>
            <begin position="112"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF12166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000006420.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="188" mass="21607" checksum="8807311345BF1C34" modified="2012-04-18" version="2">MNWTFTKTSMSISEWLKLWSIHAEVFELKCWRSFEEEYPSQRGSAKAKWVQYLIGGSLVFLIFFIIWFPLLLMNIALTIGGVTNNPTQVDVKITLGSFEPLFQVIADSDDIKIWTESQFDNFQQDFSVSQAAAAFLSGFQFDQVVTVTVDGESKTIWTISPPQYELLVDRLSPNNTKPVRLLFEWTLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>F6R5Q7</accession>
    <name>F6R5Q7_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Bromo domain-containing protein</fullName>
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    </protein>
    <organism evidence="7 8">
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        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
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    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
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                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
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                <person name="Endo T."/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
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                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
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                <person name="Oishi K."/>
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                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
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                <person name="Doggett N."/>
                <person name="Glavina T."/>
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                <person name="Richardson P."/>
                <person name="Lucas S."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <consortium name="Ensembl"/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01002411">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6SAE2"/>
    <dbReference type="Ensembl" id="ENSCINT00000014388.2">
        <property type="protein sequence ID" value="ENSCINP00000014388.2"/>
        <property type="gene ID" value="ENSCING00000007004.2"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2031784_0_0_1"/>
    <dbReference type="InParanoid" id="F6SAE2"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000014388.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="122" mass="14250" checksum="D3E54532326488F5" modified="2011-07-27" version="1">MAVCSMRLLTVVPPGFLCIFGQMPTGTCYPRMCGRSIVVGFWCGSLLELFRANPAFQVLIYSSQVIRCARWFRFWLFRLLGMWSLMPAFTLVRFFLIWRLLWLHHVTVSFWVCCRSVVASIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>F6SPA0</accession>
    <accession>A0A1W2W9B9</accession>
    <name>F6SPA0_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized LOC100177824</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100177824</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
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                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002122352.1">
        <property type="nucleotide sequence ID" value="XM_002122316.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6SPA0"/>
    <dbReference type="Ensembl" id="ENSCINT00000006824.3">
        <property type="protein sequence ID" value="ENSCINP00000006824.3"/>
        <property type="gene ID" value="ENSCING00000003320.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100177824"/>
    <dbReference type="KEGG" id="cin:100177824"/>
    <dbReference type="GeneTree" id="ENSGT00940000154225"/>
    <dbReference type="HOGENOM" id="CLU_385851_0_0_1"/>
    <dbReference type="InParanoid" id="F6SPA0"/>
    <dbReference type="OMA" id="KIGWYRF"/>
    <dbReference type="OrthoDB" id="10063988at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0005509">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="entry name" value="Zona pellucida, ZP-C domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026823">
        <property type="entry name" value="cEGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
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        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
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        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
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        <property type="entry name" value="EGF_Ca-bd_CS"/>
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        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
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    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
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        <property type="entry name" value="ZP-C_dom"/>
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    <dbReference type="InterPro" id="IPR001507">
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR24040:SF16">
        <property type="entry name" value="FIBRILLIN-2-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24040">
        <property type="entry name" value="LAMININ G-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12662">
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        <property type="match status" value="1"/>
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    <keyword evidence="3 7" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
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        <location>
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        <source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>F6TBV6</accession>
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            <fullName evidence="5">DH domain-containing protein</fullName>
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        <name type="synonym">Ascidia intestinalis</name>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
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                <person name="Haga S."/>
                <person name="Hayashi H."/>
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                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
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                <person name="Richardson P."/>
                <person name="Lucas S."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
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            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
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                <person name="Makabe K.W."/>
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                <person name="Yoshizaki F."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
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                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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                <person name="Satou Y."/>
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                <person name="Goodstein D.M."/>
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                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>4,8-dimethylnonanoyl-CoA + (R)-carnitine = O-4,8-dimethylnonanoyl-(R)-carnitine + CoA</text>
            <dbReference type="Rhea" id="RHEA:44860"/>
            <dbReference type="ChEBI" id="CHEBI:16347"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:77061"/>
            <dbReference type="ChEBI" id="CHEBI:84654"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>octanoyl-CoA + (R)-carnitine = O-octanoyl-(R)-carnitine + CoA</text>
            <dbReference type="Rhea" id="RHEA:17177"/>
            <dbReference type="ChEBI" id="CHEBI:16347"/>
            <dbReference type="ChEBI" id="CHEBI:18102"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57386"/>
            <dbReference type="EC" id="2.3.1.137"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; fatty acid beta-oxidation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the carnitine/choline acetyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.137" evidence="13"/>
    <dbReference type="AlphaFoldDB" id="F6TS86"/>
    <dbReference type="FunCoup" id="F6TS86">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000000126"/>
    <dbReference type="Ensembl" id="ENSCINT00000000126.3">
        <property type="protein sequence ID" value="ENSCINP00000000126.3"/>
        <property type="gene ID" value="ENSCING00000004324.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286999"/>
    <dbReference type="HOGENOM" id="CLU_013513_5_3_1"/>
    <dbReference type="InParanoid" id="F6TS86"/>
    <dbReference type="OMA" id="DVWAKDY"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008458">
        <property type="term" value="F:carnitine O-octanoyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.70:FF:000006">
        <property type="entry name" value="Peroxisomal carnitine O-octanoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.70">
        <property type="entry name" value="Choline/Carnitine o-acyltransferase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000542">
        <property type="entry name" value="Carn_acyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039551">
        <property type="entry name" value="Cho/carn_acyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042231">
        <property type="entry name" value="Cho/carn_acyl_trans_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22589">
        <property type="entry name" value="CARNITINE O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22589:SF67">
        <property type="entry name" value="PEROXISOMAL CARNITINE O-OCTANOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00755">
        <property type="entry name" value="Carn_acyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Choline/carnitine acyltransferase" evidence="16">
        <location>
            <begin position="14"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="15">
        <location>
            <position position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00052326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00066418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00067184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR600542-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000000126.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="603" mass="68582" checksum="5CEE17536B1C4BC1" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>F6U2I3</accession>
    <name>F6U2I3_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">wild type</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01000492">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6U2I3"/>
    <dbReference type="STRING" id="7719.ENSCINP00000011822"/>
    <dbReference type="Ensembl" id="ENSCINT00000011822.3">
        <property type="protein sequence ID" value="ENSCINP00000011822.3"/>
        <property type="gene ID" value="ENSCING00000005726.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164700"/>
    <dbReference type="HOGENOM" id="CLU_002678_2_1_1"/>
    <dbReference type="InParanoid" id="F6U2I3"/>
    <dbReference type="OMA" id="ELTGHIC"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24379:SF121">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24379">
        <property type="entry name" value="KRAB AND ZINC FINGER DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="6">
        <location>
            <begin position="115"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="6">
        <location>
            <begin position="180"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="6">
        <location>
            <begin position="208"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="6">
        <location>
            <begin position="265"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="6">
        <location>
            <begin position="293"/>
            <end position="316"/>
        </location>
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    <sequence length="377" mass="43320" checksum="F5547748F0533525" modified="2012-04-18" version="2">MKHHTKHCVKVVSGSYEAGTLMKYSHDPTTFTCILDDGNCDVKCKKQHEMCQHLLNTHGITSFPCKYCPAVLKDSASLQSHKLLHQTYICLYCDYSTTSKNRFVKHYFQKHGTDFKCSFCNKKCNSKFELDSHETLHILQPIDSNSICYKCLLCSYVTPHQEVSLLKKHIMRKHVANETFQCTKCSYTCNEKSQLNLHMRKHSQEKPYLCQLCGFMCKWSSQMNLHQAYHTGLKLHKCPHCQYKSYRADTLKIHLKHKHGFARTKLCDKCGYAATCASAFKVHVAGHSKQRPYTCQFCRKTYKTRGNLRAHQRKAHKELVRIKLQSQTTTTTDIVPAMLPCHQNNSINSEPLDIIGAEVDTESIDQGEDTRLGTEIA</sequence>
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        <name type="scientific">Ciona intestinalis</name>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
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                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
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                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
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                <person name="Hayashi H."/>
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                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
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                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
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                <person name="Nishizaka S."/>
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                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
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                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
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                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="12">wild type</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Di Gregorio A."/>
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                <person name="Lemaire P."/>
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                <person name="Meinertzhagen I.A."/>
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                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
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                <person name="Richardson P."/>
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    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
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                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
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                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">wild type</strain>
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    <reference evidence="2" key="3">
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        <scope>IDENTIFICATION</scope>
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    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000022848.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="177" mass="20059" checksum="45AE6BF6FDD91ED3" modified="2012-04-18" version="2">MTFTDMNDWIGSRYLREMAAIDNLSSVVRQCIEEGVQLEYELLLEQDEFIINHDAKVIRTPSPPPRPEPIETSQPDVFTIVQLGKLYSHFKGVAPNGMILNKAFIEVLADTAALDAGADALPDMWINISYNQLVELADLLSGESEYIDWRKFLLLAAFPWQQPTTEELLLARSAFQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>F6UN42</accession>
    <accession>A0A1W2WED3</accession>
    <name>F6UN42_CIOIN</name>
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        <submittedName>
            <fullName evidence="1">Protein D2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">LOC100175630</name>
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    <organism evidence="1 2">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">wild type</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01000092">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002129723.1">
        <property type="nucleotide sequence ID" value="XM_002129687.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6UN42"/>
    <dbReference type="FunCoup" id="F6UN42">
        <property type="interactions" value="11"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000001993"/>
    <dbReference type="Ensembl" id="ENSCINT00000001993.3">
        <property type="protein sequence ID" value="ENSCINP00000001993.3"/>
        <property type="gene ID" value="ENSCING00000001057.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100175630"/>
    <dbReference type="KEGG" id="cin:100175630"/>
    <dbReference type="GeneTree" id="ENSGT00940000167139"/>
    <dbReference type="HOGENOM" id="CLU_043994_5_1_1"/>
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    <dbReference type="OMA" id="QEVICYE"/>
    <dbReference type="OrthoDB" id="2506647at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PEBP"/>
    </dbReference>
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        <property type="entry name" value="PEBP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035810">
        <property type="entry name" value="PEBP_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11362:SF147">
        <property type="entry name" value="PHOSPHATIDYLETHANOLAMINE BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11362">
        <property type="entry name" value="PHOSPHATIDYLETHANOLAMINE-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PBP"/>
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    </dbReference>
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        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000001993.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="177" mass="20132" checksum="6494F3C95B34DA6E" modified="2011-07-27" version="1">MESAWSKNELDQVEDCPKEQANVKFNDFHLNEIGQITTPTVVQNEPTVTWTTENGKLYSIFMTDPDAPSRAEPKFREWYHWGVINIPGTNIKEGQVVAEYVGAGPPEGTGLHRYVFLVYEQNEKVETSDKIGMVMKGRDTQKIQDIAKKYKLGCLVAAACFQAQYDDYVPLLYAKFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>F6V1Q0</accession>
    <name>F6V1Q0_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Calx-beta domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Phlebobranchia</taxon>
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            <taxon>Ciona</taxon>
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    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">wild type</strain>
        </source>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>Ca(2+)(in) + 3 Na(+)(out) = Ca(2+)(out) + 3 Na(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:69955"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19) family. SLC8 subfamily.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="ENSCINP00000012945.3"/>
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        <property type="component" value="Chromosome 10"/>
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    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0000318"/>
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        <property type="project" value="GO_Central"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="GO_Central"/>
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        <property type="match status" value="2"/>
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        <property type="entry name" value="NCX, central ion-binding region"/>
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        <property type="entry name" value="CalX-like_sf"/>
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        <property type="entry name" value="caca"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="NA_CA-EXCHANGE PROTEIN, ISOFORM G"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR11878">
        <property type="entry name" value="SODIUM/CALCIUM EXCHANGER"/>
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    <dbReference type="Pfam" id="PF03160">
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    <keyword evidence="6" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="12" id="KW-0112">Calmodulin-binding</keyword>
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    <keyword evidence="16 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
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    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="14" id="KW-0915">Sodium</keyword>
    <keyword evidence="18" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="7 21" id="KW-0812">Transmembrane</keyword>
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            <end position="46"/>
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    <feature type="transmembrane region" description="Helical" evidence="21">
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    <feature type="transmembrane region" description="Helical" evidence="21">
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            <end position="202"/>
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    <feature type="transmembrane region" description="Helical" evidence="21">
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        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
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    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00033667"/>
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        <source>
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        <source>
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        <source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="895" mass="98479" checksum="2DEBF166F063A2FE" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>F6VAW2</accession>
    <name>F6VAW2_CIOIN</name>
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        <recommendedName>
            <fullName evidence="17">Zinc finger protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
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        <property type="protein sequence ID" value="ENSCINP00000010583.3"/>
        <property type="gene ID" value="ENSCING00000005141.3"/>
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        <property type="project" value="GO_Central"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="GO_Central"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="GO_Central"/>
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        <property type="project" value="GO_Central"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
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        <property type="entry name" value="CUE"/>
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        <property type="entry name" value="Znf_RING"/>
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        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
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        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE AMFR"/>
        <property type="match status" value="1"/>
    </dbReference>
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            <end position="104"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="44" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="44">wild type</strain>
        </source>
    </reference>
    <reference evidence="44" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="44" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="32">
            <text>(3S)-3-hydroxydodecanoyl-CoA + NAD(+) = 3-oxododecanoyl-CoA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:31179"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:62558"/>
            <dbReference type="ChEBI" id="CHEBI:62615"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="32">
            <dbReference type="Rhea" id="RHEA:31180"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>(3S)-3-hydroxydodecanoyl-CoA = (2E)-dodecenoyl-CoA + H2O</text>
            <dbReference type="Rhea" id="RHEA:31075"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57330"/>
            <dbReference type="ChEBI" id="CHEBI:62558"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="29">
            <dbReference type="Rhea" id="RHEA:31077"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>(3S)-hydroxydecanoyl-CoA + NAD(+) = 3-oxodecanoyl-CoA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:31187"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:62548"/>
            <dbReference type="ChEBI" id="CHEBI:62616"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:31188"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>(3S)-hydroxyhexadecanoyl-CoA + NAD(+) = 3-oxohexadecanoyl-CoA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:31159"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57349"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:62613"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:31160"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(3S)-hydroxyhexadecanoyl-CoA = (2E)-hexadecenoyl-CoA + H2O</text>
            <dbReference type="Rhea" id="RHEA:31163"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:61526"/>
            <dbReference type="ChEBI" id="CHEBI:62613"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="1">
            <dbReference type="Rhea" id="RHEA:31165"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>(3S)-hydroxyoctanoyl-CoA + NAD(+) = 3-oxooctanoyl-CoA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:31195"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:62617"/>
            <dbReference type="ChEBI" id="CHEBI:62619"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="28">
            <dbReference type="Rhea" id="RHEA:31196"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>(3S)-hydroxyoctanoyl-CoA = (2E)-octenoyl-CoA + H2O</text>
            <dbReference type="Rhea" id="RHEA:31199"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:62242"/>
            <dbReference type="ChEBI" id="CHEBI:62617"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="27">
            <dbReference type="Rhea" id="RHEA:31201"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="30">
            <text>(3S)-hydroxytetradecanoyl-CoA + NAD(+) = 3-oxotetradecanoyl-CoA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:31167"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:62543"/>
            <dbReference type="ChEBI" id="CHEBI:62614"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="30">
            <dbReference type="Rhea" id="RHEA:31168"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="31">
            <text>1'-[1,2-di-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phospho]-3'-[1-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phospho]-glycerol + (9Z)-octadecenoyl-CoA = 1'-[1,2-di-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phospho]-3'-[1-(9Z,12Z-octadecadienoyl)-2-(9Z-octadecenoyl)-sn-glycero-3-phospho]-glycerol + CoA</text>
            <dbReference type="Rhea" id="RHEA:43676"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57387"/>
            <dbReference type="ChEBI" id="CHEBI:83580"/>
            <dbReference type="ChEBI" id="CHEBI:83582"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="31">
            <dbReference type="Rhea" id="RHEA:43677"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>1'-[1,2-di-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phospho]-3'-[1-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phospho]-glycerol + (9Z,12Z)-octadecadienoyl-CoA = 1',3'-bis-[1,2-di-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phospho]-glycerol + CoA</text>
            <dbReference type="Rhea" id="RHEA:43672"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57383"/>
            <dbReference type="ChEBI" id="CHEBI:83580"/>
            <dbReference type="ChEBI" id="CHEBI:83581"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:43673"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="33">
            <text>1'-[1,2-di-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phospho]-3'-[1-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phospho]-glycerol + hexadecanoyl-CoA = 1'-[1,2-di-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phospho]-3'-[1-(9Z,12Z-octadecadienoyl)-2-hexadecanoyl-sn-glycero-3-phospho]-glycerol + CoA</text>
            <dbReference type="Rhea" id="RHEA:43680"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:83580"/>
            <dbReference type="ChEBI" id="CHEBI:83583"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="33">
            <dbReference type="Rhea" id="RHEA:43681"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>a (3S)-3-hydroxyacyl-CoA = a (2E)-enoyl-CoA + H2O</text>
            <dbReference type="Rhea" id="RHEA:16105"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57318"/>
            <dbReference type="ChEBI" id="CHEBI:58856"/>
            <dbReference type="EC" id="4.2.1.17"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="21">
            <dbReference type="Rhea" id="RHEA:16107"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>a 4-saturated-(3S)-3-hydroxyacyl-CoA = a (3E)-enoyl-CoA + H2O</text>
            <dbReference type="Rhea" id="RHEA:20724"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58521"/>
            <dbReference type="ChEBI" id="CHEBI:137480"/>
            <dbReference type="EC" id="4.2.1.17"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="26">
            <dbReference type="Rhea" id="RHEA:20726"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>a long-chain (3S)-3-hydroxy fatty acyl-CoA + NAD(+) = a long-chain 3-oxo-fatty acyl-CoA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52656"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:136757"/>
            <dbReference type="ChEBI" id="CHEBI:136758"/>
            <dbReference type="EC" id="1.1.1.211"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:52657"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism; fatty acid beta-oxidation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="34">Heterotetramer of 2 alpha/HADHA and 2 beta/HADHB subunits; forms the mitochondrial trifunctional enzyme. Also purified as higher order heterooligomers including a 4 alpha/HADHA and 4 beta/HADHB heterooligomer which physiological significance remains unclear. The mitochondrial trifunctional enzyme interacts with MTLN.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="41">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the central section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.211" evidence="35"/>
    <dbReference type="EC" id="4.2.1.17" evidence="6"/>
    <dbReference type="EMBL" id="EAAA01000059">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6VRU1"/>
    <dbReference type="FunCoup" id="F6VRU1">
        <property type="interactions" value="219"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000012644"/>
    <dbReference type="Ensembl" id="ENSCINT00000012644.3">
        <property type="protein sequence ID" value="ENSCINP00000012644.3"/>
        <property type="gene ID" value="ENSCING00000006084.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154677"/>
    <dbReference type="HOGENOM" id="CLU_009834_16_1_1"/>
    <dbReference type="InParanoid" id="F6VRU1"/>
    <dbReference type="OMA" id="ESTTIRW"/>
    <dbReference type="UniPathway" id="UPA00659"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016507">
        <property type="term" value="C:mitochondrial fatty acid beta-oxidation multienzyme complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004300">
        <property type="term" value="F:enoyl-CoA hydratase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016509">
        <property type="term" value="F:long-chain (3S)-3-hydroxyacyl-CoA dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000011">
        <property type="entry name" value="Fatty acid oxidation complex subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000009">
        <property type="entry name" value="Fatty oxidation complex, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1040.50:FF:000002">
        <property type="entry name" value="Trifunctional enzyme subunit alpha, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006176">
        <property type="entry name" value="3-OHacyl-CoA_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006108">
        <property type="entry name" value="3HC_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018376">
        <property type="entry name" value="Enoyl-CoA_hyd/isom_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012803">
        <property type="entry name" value="Fa_ox_alpha_mit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050136">
        <property type="entry name" value="FA_oxidation_alpha_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02441">
        <property type="entry name" value="fa_ox_alpha_mit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43612">
        <property type="entry name" value="TRIFUNCTIONAL ENZYME SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43612:SF3">
        <property type="entry name" value="TRIFUNCTIONAL ENZYME SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00725">
        <property type="entry name" value="3HCDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02737">
        <property type="entry name" value="3HCDH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00166">
        <property type="entry name" value="ENOYL_COA_HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0007">Acetylation</keyword>
    <keyword evidence="11" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="16" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="19" id="KW-0456">Lyase</keyword>
    <keyword evidence="18" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0488">Methylation</keyword>
    <keyword evidence="17" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="20" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="15" id="KW-0520">NAD</keyword>
    <keyword evidence="14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="45" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="12" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="3-hydroxyacyl-CoA dehydrogenase NAD binding" evidence="43">
        <location>
            <begin position="323"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="domain" description="3-hydroxyacyl-CoA dehydrogenase C-terminal" evidence="42">
        <location>
            <begin position="504"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="active site" description="For hydroxyacyl-coenzyme A dehydrogenase activity" evidence="39">
        <location>
            <position position="470"/>
        </location>
    </feature>
    <feature type="site" description="Important for long-chain enoyl-CoA hydratase activity" evidence="40">
        <location>
            <position position="109"/>
        </location>
    </feature>
    <feature type="site" description="Important for long-chain enoyl-CoA hydratase activity" evidence="40">
        <location>
            <position position="131"/>
        </location>
    </feature>
    <feature type="site" description="Important for hydroxyacyl-coenzyme A dehydrogenase activity" evidence="40">
        <location>
            <position position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00035854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00048361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00050222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00050446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00051215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00051877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00052224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00052711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00052834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00052860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00052945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="ARBA" id="ARBA00052989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="ARBA" id="ARBA00062153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="35">
        <source>
            <dbReference type="ARBA" id="ARBA00066806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="36">
        <source>
            <dbReference type="ARBA" id="ARBA00068347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="37">
        <source>
            <dbReference type="ARBA" id="ARBA00077617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="38">
        <source>
            <dbReference type="ARBA" id="ARBA00083277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="39">
        <source>
            <dbReference type="PIRSR" id="PIRSR612803-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="40">
        <source>
            <dbReference type="PIRSR" id="PIRSR612803-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="41">
        <source>
            <dbReference type="RuleBase" id="RU003707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="42">
        <source>
            <dbReference type="Pfam" id="PF00725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="43">
        <source>
            <dbReference type="Pfam" id="PF02737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="44">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000012644.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="45">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="725" mass="78079" checksum="9492BE1453F28D53" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>F6VT08</accession>
    <accession>A0A1W2WHP4</accession>
    <name>F6VT08_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Pyruvate dehydrogenase E1 component subunit alpha</fullName>
            <ecNumber evidence="10">1.2.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100184477</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">wild type</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 10">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[protein] + pyruvate + H(+) = N(6)-[(R)-S(8)-acetyldihydrolipoyl]-L-lysyl-[protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:19189"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:10478"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83111"/>
            <dbReference type="EC" id="1.2.4.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="9">Heterotetramer of two PDHA2 and two PDHB subunits. The heterotetramer interacts with DLAT, and is part of the multimeric pyruvate dehydrogenase complex that contains multiple copies of pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (DLAT, E2) and lipoamide dehydrogenase (DLD, E3). These subunits are bound to an inner core composed of about 48 DLAT and 12 PDHX molecules.</text>
    </comment>
    <dbReference type="EC" id="1.2.4.1" evidence="10"/>
    <dbReference type="EMBL" id="EAAA01000920">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002131120.1">
        <property type="nucleotide sequence ID" value="XM_002131084.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6VT08"/>
    <dbReference type="FunCoup" id="F6VT08">
        <property type="interactions" value="110"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000012352"/>
    <dbReference type="Ensembl" id="ENSCINT00000012352.3">
        <property type="protein sequence ID" value="ENSCINP00000012352.3"/>
        <property type="gene ID" value="ENSCING00000010459.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100184477"/>
    <dbReference type="KEGG" id="cin:100184477"/>
    <dbReference type="GeneTree" id="ENSGT00530000063174"/>
    <dbReference type="HOGENOM" id="CLU_029393_5_2_1"/>
    <dbReference type="InParanoid" id="F6VT08"/>
    <dbReference type="OMA" id="LGYEMPC"/>
    <dbReference type="OrthoDB" id="10256198at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004739">
        <property type="term" value="F:pyruvate dehydrogenase (acetyl-transferring) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006086">
        <property type="term" value="P:pyruvate decarboxylation to acetyl-CoA"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02000">
        <property type="entry name" value="TPP_E1_PDC_ADC_BCADC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000013">
        <property type="entry name" value="Pyruvate dehydrogenase E1 component subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001017">
        <property type="entry name" value="DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050642">
        <property type="entry name" value="PDH_E1_Alpha_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017597">
        <property type="entry name" value="Pyrv_DH_E1_asu_subgrp-y"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03182">
        <property type="entry name" value="PDH_E1_alph_y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11516:SF60">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE E1 COMPONENT SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11516">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE E1 COMPONENT, ALPHA SUBUNIT BACTERIAL AND ORGANELLAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00676">
        <property type="entry name" value="E1_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="3" id="KW-0313">Glucose metabolism</keyword>
    <keyword evidence="5 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7 10" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Dehydrogenase E1 component" evidence="11">
        <location>
            <begin position="61"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00051231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00062595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000012352.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="385" mass="42963" checksum="277538C324A32C1F" modified="2012-04-18" version="2">MSYNATAKFLQRVLCKQPLGVRTYSVEATFPIKPIDTHKLENTPATEVQINKDDAISYYTKMQIIRRMELKADQLYKQKIIRGFCHLYDGQEACCAGIEASLTPKDDVITAYRAHGWAYIRGVPVCNILAELFGRKLGCAKGKGGSMHMYSKNFYGGNGIVGAQVPLGAGIAFAHKYRNSDTVTVACYGDGAANQGQIFESFNMSQLWKLPAIFVCENNRYGMGTSVERASASTDYFTRGDYIPGLRVDGMDVLAVREATRFAKEHAVKEGPILMELVTYRYHGHSMSDPGTSYRTRDEVKEIRQNLDPIMNFREKIISAGFVTKQDVATIDAEVKQHVENETAKALASTEPDFDQIACDIYVTDTPLSIRSCNIYNQLHHQPSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>F6VVA7</accession>
    <name>F6VVA7_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">wild type</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SIX/Sine oculis homeobox family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01000466">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6VVA7"/>
    <dbReference type="STRING" id="7719.ENSCINP00000012025"/>
    <dbReference type="Ensembl" id="ENSCINT00000012025.3">
        <property type="protein sequence ID" value="ENSCINP00000012025.3"/>
        <property type="gene ID" value="ENSCING00000005828.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000169263"/>
    <dbReference type="HOGENOM" id="CLU_057254_0_0_1"/>
    <dbReference type="InParanoid" id="F6VVA7"/>
    <dbReference type="OMA" id="AFHQGHY"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000046">
        <property type="entry name" value="SIX homeobox 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031701">
        <property type="entry name" value="SIX1_SD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10390">
        <property type="entry name" value="HOMEOBOX PROTEIN SIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10390:SF33">
        <property type="entry name" value="PROTEIN OPTIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16878">
        <property type="entry name" value="SIX1_SD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4 7 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 7 8" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6 7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="10">
        <location>
            <begin position="202"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="7">
        <location>
            <begin position="204"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="232"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="325"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="7"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="263"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="273"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="332"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="353"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="383"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000012025.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="403" mass="44946" checksum="94904A3D56671F0F" modified="2012-04-18" version="2">TVAQRASSPIVMSSQSSLSPSSSIAKLFSGAANSRSMFGGMGSNPRPDIVAAAAALRHMINQNLLQQPASQSPNPLLPFPALTFSPSQIATVCESLQESGDIERLARFLWSLPAAPGVLEVLNTNEVVLRARAIVAFHQGHYRDLYAILETHRFTEKDSHGKLQAMWLEAHYQEAAKLRGRPLGPVDKYRIRKKFPLPRTIWDGEQKTHCFKERTRSLLREWYLRDPYPNPSKKKELAHATGLTPTQVGNWFKNRRQRDRAAAAKNNRQQNQLSPQPRSKTTSPTSYQNELLRHYGTHNDPSAMFWAVGKFPGFPMTVPHFQRPTYIDKGDSPTGSTFSSPSFLPTPPMTSLHRLNTSSPKAESDSGSVSPISSHYDKRIESDDITNEDNFPEADTASQSPKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>F6VXS0</accession>
    <name>F6VXS0_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">V-type proton ATPase subunit F</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100186035</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">wild type</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">V-ATPase is a heteromultimeric enzyme made up of two complexes: the ATP-hydrolytic V1 complex and the proton translocation V0 complex.</text>
    </comment>
    <comment type="similarity">
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
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                <person name="Doyle S."/>
                <person name="Francino P."/>
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                <person name="Kobayashi K."/>
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                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
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                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="3" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        </location>
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        <source>
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        <source>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Manohar C."/>
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                <person name="Medina M."/>
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                <person name="Mount S."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="2" key="2">
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                <consortium name="Ensembl"/>
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                <consortium name="Ensembl"/>
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    <dbReference type="GO" id="GO:0005794">
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        <property type="evidence" value="ECO:0000318"/>
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    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
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        <property type="term" value="C:trans-Golgi network transport vesicle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0044325">
        <property type="term" value="F:transmembrane transporter binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:2000009">
        <property type="term" value="P:negative regulation of protein localization to cell surface"/>
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        <property type="entry name" value="GOPC"/>
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        <property type="entry name" value="PDZ"/>
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    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16528">
        <property type="entry name" value="GOLGI-ASSOCIATED PDZ AND COILED-COIL MOTIF-CONTAINING"/>
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    <dbReference type="PANTHER" id="PTHR16528:SF2">
        <property type="entry name" value="GOLGI-ASSOCIATED PDZ AND COILED-COIL MOTIF-CONTAINING PROTEIN"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDZ" evidence="1">
        <location>
            <begin position="256"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000017767.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="347" mass="38542" checksum="E41939A4FC294B73" modified="2012-04-18" version="2">MSNTAATFHCLEVLEKEFDKAFVDLDVLLGEVEFDESDEVIVEAREKMNTLSSCFSQLCHKTQTTAQTNAKLEADAVNTKTELITAQANFEASKVETSSLVRQLHTLQLQLHTATSADANFNPKTITDKLDTELEAFRMNARKEARYSEEINNLKQDNYAITKKVAALESELYGARLAAKYLDKELAGRIQQIQLLGRNIKPPNFEKMWHQLEAEIHLHRHKTVIRACRGATKQPSQLKQQQPVPVSNHGVGEVRKLTIQKSHEEGLGISITGGREHGVPILVSEIHEGAIVDRTGGLYVGDAILSVNGVDLRHAKHQDAVDALSQQVESVTLEAVYVNPDDGTEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>F6WIE6</accession>
    <accession>A0A1W2W6E7</accession>
    <name>F6WIE6_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized LOC100179345</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100179345</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">wild type</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLX9 family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002850">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002125233.2">
        <property type="nucleotide sequence ID" value="XM_002125197.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6WIE6"/>
    <dbReference type="FunCoup" id="F6WIE6">
        <property type="interactions" value="20"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000003689"/>
    <dbReference type="Ensembl" id="ENSCINT00000003689.3">
        <property type="protein sequence ID" value="ENSCINP00000003689.3"/>
        <property type="gene ID" value="ENSCING00000010894.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100179345"/>
    <dbReference type="KEGG" id="cin:100179345"/>
    <dbReference type="GeneTree" id="ENSGT00390000015709"/>
    <dbReference type="HOGENOM" id="CLU_1179870_0_0_1"/>
    <dbReference type="InParanoid" id="F6WIE6"/>
    <dbReference type="OMA" id="DLMFHEA"/>
    <dbReference type="OrthoDB" id="18703at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028160">
        <property type="entry name" value="Slx9-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31109">
        <property type="entry name" value="PROTEIN FAM207A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31109:SF2">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN SLX9 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15341">
        <property type="entry name" value="SLX9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="164"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="173"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000003689.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="235" mass="27310" checksum="D7EB29353D86E063" modified="2011-07-27" version="1">MGKVRSKKSRIHLKATKVATSDKEIEPEESNDYNNNDKEEILPNLENNPFFGLKIDPALLTKQSLQESEIENNDASGKTKFLSKKRVTESFGETVKTKKEKMNRRREMFLQKLEVNQRIAKKQKQAKQRAKNPVVGDMHGLKLALTEIPKLTSEIPIVIAKTKKKSRRKRKKESSLQKVEKVSLTDKDMRRVRSKRTQKHMLNEIAKLQSMMKDKSYQSNSKEIILKNIKQHLIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>F6WJF4</accession>
    <name>F6WJF4_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the copine family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="F6WJF4"/>
    <dbReference type="FunCoup" id="F6WJF4">
        <property type="interactions" value="29"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000003664"/>
    <dbReference type="Ensembl" id="ENSCINT00000003664.3">
        <property type="protein sequence ID" value="ENSCINP00000003664.3"/>
        <property type="gene ID" value="ENSCING00000001809.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159679"/>
    <dbReference type="HOGENOM" id="CLU_020452_3_2_1"/>
    <dbReference type="InParanoid" id="F6WJF4"/>
    <dbReference type="OMA" id="QCANVPI"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071277">
        <property type="term" value="P:cellular response to calcium ion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04048">
        <property type="entry name" value="C2A_Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04047">
        <property type="entry name" value="C2B_Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Copine 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037768">
        <property type="entry name" value="C2B_Copine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045052">
        <property type="entry name" value="Copine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010734">
        <property type="entry name" value="Copine_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10857:SF106">
        <property type="entry name" value="C2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10857">
        <property type="entry name" value="COPINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07002">
        <property type="entry name" value="Copine"/>
        <property type="match status" value="1"/>
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    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="C2" evidence="4">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="442"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="455"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000003664.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="464" mass="52073" checksum="56964BB2E21371A4" modified="2012-04-18" version="2">MAIELGLSCRNLIDADVFSKSDPMVVMYTQGLGSKEWREFGRTETIWNNLNPDFVKKFIIDYFFEERQAMRFDVYDVDSKSPDLSKHDFLGQVFCSLGEVVIHGTCNNQRINKITFPHRGTSSRNTGRIVIMAEELDSSKDIIRMKFNGRKLDKKDLFGKSDPFMVFCRCNENNSFTVCHKTEVKKNTLNPEWQLIEIPVRTLCNGDHERTIKVTIDFTASNGNPNQPSSLHYIHPHQPNQYALAIQAVGEIVQDYDSDKLFPTFGFGARLPPDGRVSHEFALNFNPANPNCFGIQGVMEAYQSSIRSVQLYGPTNFSPIINQVSRIAAGDTSGSQYYILLMITDGVISDMEATKEAIVNAARLPMSIIIVGVGPAEFDAMEELDGDTVRLSYRGIEAERDIVQFVPFRDYVAQGKSQILSQARLSKDVLAEIPDQLVGYMKSRNIQPGPPPPTYTQQPGPSAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>F6WPT0</accession>
    <accession>A0A1W3JHY1</accession>
    <name>F6WPT0_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Nuclear receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">ftzf</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">wild type</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nuclear hormone receptor family. NR5 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002261">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009858805.1">
        <property type="nucleotide sequence ID" value="XM_009860503.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6WPT0"/>
    <dbReference type="STRING" id="7719.ENSCINP00000009479"/>
    <dbReference type="Ensembl" id="ENSCINT00000009479.3">
        <property type="protein sequence ID" value="ENSCINP00000009479.3"/>
        <property type="gene ID" value="ENSCING00000004593.3"/>
    </dbReference>
    <dbReference type="GeneID" id="778629"/>
    <dbReference type="CTD" id="778629"/>
    <dbReference type="GeneTree" id="ENSGT00940000153391"/>
    <dbReference type="HOGENOM" id="CLU_011437_0_0_1"/>
    <dbReference type="InParanoid" id="F6WPT0"/>
    <dbReference type="OMA" id="HQEFACI"/>
    <dbReference type="OrthoDB" id="5771769at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090575">
        <property type="term" value="C:RNA polymerase II transcription regulator complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004879">
        <property type="term" value="F:nuclear receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009755">
        <property type="term" value="P:hormone-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009888">
        <property type="term" value="P:tissue development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07167">
        <property type="entry name" value="NR_DBD_Lrh-1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Nuclear receptor subfamily 5 group A member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.565.10:FF:000011">
        <property type="entry name" value="Nuclear receptor subfamily 5, group A, member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.565.10">
        <property type="entry name" value="Retinoid X Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NHR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016355">
        <property type="entry name" value="NR5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000536">
        <property type="entry name" value="Nucl_hrmn_rcpt_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001723">
        <property type="entry name" value="Nuclear_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001628">
        <property type="entry name" value="Znf_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24086:SF15">
        <property type="entry name" value="NUCLEAR HORMONE RECEPTOR FTZ-F1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24086">
        <property type="entry name" value="NUCLEAR RECEPTOR SUBFAMILY 5 GROUP A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00104">
        <property type="entry name" value="Hormone_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00105">
        <property type="entry name" value="zf-C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002530">
        <property type="entry name" value="Nuc_orph_FTZ-F1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00398">
        <property type="entry name" value="STRDHORMONER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00047">
        <property type="entry name" value="STROIDFINGER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00430">
        <property type="entry name" value="HOLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00399">
        <property type="entry name" value="ZnF_C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48508">
        <property type="entry name" value="Nuclear receptor ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00031">
        <property type="entry name" value="NUCLEAR_REC_DBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51030">
        <property type="entry name" value="NUCLEAR_REC_DBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5 12" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Nuclear receptor" evidence="13">
        <location>
            <begin position="6"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="NR LBD" evidence="14">
        <location>
            <begin position="271"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="477"/>
        </location>
        <ligand>
            <name>a phospholipid derivative</name>
            <dbReference type="ChEBI" id="CHEBI:16247"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="481"/>
        </location>
        <ligand>
            <name>a phospholipid derivative</name>
            <dbReference type="ChEBI" id="CHEBI:16247"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR002530-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000009479.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="504" mass="57214" checksum="A4A45B435DBCBB95" modified="2011-07-27" version="1">MKYSLDEVCPVCGDKVSGYHYGLLTCESCKGFFKRTVQNGKKYTCVHTSKCHIDKDQRKGCQACRFQKCLRVGMKLEAVRRDRVRGGRNKFGPLYKQDRARKQRLKALSEVHDSSTTITNLTLSVETTKVEFCEPVPLIQTPTPTNNECYVTPSHDSIWPSSLESPSTSSIMSVLPQPYISHSPSPNTSSLGSTGDLTVRNISGNSYIEPPTFHPPPDPMQTHYPLLWGAHPAMNPTLPPTYLSMGPIPAPYQLLPFSPNSVIEGFQRGFYVPLLIDQLLKYEIVESEIKTRIGTSLLDVTHRSDVFQLICKIADQTLYAMVEWAKKSVFFADLQVSDQMKLLQDSWIELLILDHIQRQLKLKSDSIELVTGHKFPRDLSKVSSHCVITFRILKRLMDLEKKFAVLQVDEQEIACLKYLVLFDTESHTAGCPNVQEKVNEALCEYSKKNYMDQPDRVNKLIFRLSEIRAVSSQIEDYLHYKHHEGKLTEGSLLVELLKARENGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>F6WZV6</accession>
    <name>F6WZV6_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>Na(+)(in) = Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34963"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the potassium channel HCN family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="F6WZV6"/>
    <dbReference type="FunCoup" id="F6WZV6">
        <property type="interactions" value="7"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSCINT00000017729.3">
        <property type="protein sequence ID" value="ENSCINP00000017729.3"/>
        <property type="gene ID" value="ENSCING00000008692.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000167392"/>
    <dbReference type="HOGENOM" id="CLU_005746_15_2_1"/>
    <dbReference type="InParanoid" id="F6WZV6"/>
    <dbReference type="OMA" id="YFVQHGC"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098855">
        <property type="term" value="C:HCN channel complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030552">
        <property type="term" value="F:cAMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005272">
        <property type="term" value="F:sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071805">
        <property type="term" value="P:potassium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003254">
        <property type="term" value="P:regulation of membrane depolarization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.630">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013621">
        <property type="entry name" value="Ion_trans_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051413">
        <property type="entry name" value="K/Na_HCN_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003938">
        <property type="entry name" value="K_chnl_volt-dep_EAG/ELK/ERG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689:SF5">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08412">
        <property type="entry name" value="Ion_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01463">
        <property type="entry name" value="EAGCHANLFMLY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00888">
        <property type="entry name" value="CNMP_BINDING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0114">cAMP</keyword>
    <keyword evidence="6" id="KW-0116">cAMP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="16" id="KW-0407">Ion channel</keyword>
    <keyword evidence="11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="15" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="12 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0915">Sodium</keyword>
    <keyword evidence="4" id="KW-0894">Sodium channel</keyword>
    <keyword evidence="14" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="7 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="42"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="116"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="157"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="202"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="279"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="19">
        <location>
            <begin position="381"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00036239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000017729.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="499" mass="58475" checksum="18178C01EB46F9AC" modified="2012-04-18" version="2">MNKMSLKLYGSEKAVIEEQERLKKAGSWIIHPYSNFRFIWDSFTLVLLLVNIILIPVIISFWKDDDSAWLPFKAVSDTWFLTDIIINFRTGIVIDGPDSEVILDPKQIRMMYLKTWFTIDLVSTFPFDLVFTIIYTLFKSLERNDGASSMAETGLKALSLLRFAKILALLRLLRLSRFIRYMKQWEEIFNFQYEMALAFARIFNLIMLMLFICHFNGCLQYMVPMFLDFPEKTWVRDRNLHLSNVTIWERYSWSVFKSTSHMLCIGYGLFPPQGLVDVWVTYVSMCSGAMCFALFIGNATSLIQSMDASKRAYKEKYMQVKEYMQFRKLPSGLRHRISDYYENRFQGKMFDEERILTELNHNLRDEVIQYNCKDLVDQVPFFNEADPSFVAAMLGKLNFEVFLNDEVIVKEGTEGKKMYFINRGTVTIKSAQHKIEQSLSDGCYFGEIALLQQNLRRVASVTAETYCYLYSLSVDDFNEVLKEFPRQRAKLQHVAQARL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>F6XFJ7</accession>
    <name>F6XFJ7_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">[histone H3]-trimethyl-L-lysine(9) demethylase</fullName>
            <ecNumber evidence="3">1.14.11.66</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
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                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <reaction evidence="14">
            <text>N(6),N(6),N(6)-trimethyl-L-lysyl(9)-[histone H3] + 2 2-oxoglutarate + 2 O2 = N(6)-methyl-L-lysyl(9)-[histone H3] + 2 formaldehyde + 2 succinate + 2 CO2</text>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
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                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Ogasawara M."/>
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                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
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                <person name="Wiley G.B."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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                <consortium name="Ensembl"/>
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        <property type="entry name" value="Nas_poly-pep-assoc_cplx_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21713">
        <property type="entry name" value="NASCENT POLYPEPTIDE ASSOCIATED COMPLEX ALPHA SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01849">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19026">
        <property type="entry name" value="UBA_HYPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015901">
        <property type="entry name" value="NAC_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01407">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51151">
        <property type="entry name" value="NAC_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAC-A/B" evidence="2">
        <location>
            <begin position="64"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000003937.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="206" mass="22290" checksum="9A1E01BF13F7A3C8" modified="2012-04-18" version="2">EAVESLSATSKEKIQEIVEADSATESDSDDESMPDLDENPDVPMTTTADMLGGGDASGGKSKQSRSEKKARKTLSKLGLKLVTGINRVTIRKSKNILFVINRPDIYKNPVSDTYIVFGEAKIEDLSQQAQLAAAEKFKNKSTTATESATAVNAVQEESDEEVDESEVETKDVELVMQQASVSRSKAVKALKNNGNDIVNAIMELTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>F6Y1R8</accession>
    <name>F6Y1R8_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 14">Phosphoinositide phospholipase C</fullName>
            <ecNumber evidence="2 14">3.1.4.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC100185660</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">wild type</strain>
        </source>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + H2O = 1D-myo-inositol 1,4,5-trisphosphate + a 1,2-diacyl-sn-glycerol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:33179"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
            <dbReference type="ChEBI" id="CHEBI:203600"/>
            <dbReference type="EC" id="3.1.4.11"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:33180"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="12">Binds 3 Ca(2+) ions per subunit. Two of the Ca(2+) ions are bound to the C2 domain.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.1.4.11" evidence="2 14"/>
    <dbReference type="EMBL" id="EAAA01002849">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6Y1R8"/>
    <dbReference type="STRING" id="7719.ENSCINP00000009407"/>
    <dbReference type="Ensembl" id="ENSCINT00000009407.3">
        <property type="protein sequence ID" value="ENSCINP00000009407.3"/>
        <property type="gene ID" value="ENSCING00000004554.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000167636"/>
    <dbReference type="InParanoid" id="F6Y1R8"/>
    <dbReference type="OMA" id="RMVAMHA"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004435">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate phospholipase C activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00275">
        <property type="entry name" value="C2_PLC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16202">
        <property type="entry name" value="EFh_PI-PLCdelta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08593">
        <property type="entry name" value="PI-PLCc_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000005">
        <property type="entry name" value="Phosphoinositide phospholipase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000910">
        <property type="entry name" value="Phosphoinositide phospholipase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000509">
        <property type="entry name" value="Phosphoinositide phospholipase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.190:FF:000039">
        <property type="entry name" value="Phosphoinositide phospholipase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001192">
        <property type="entry name" value="PI-PLC_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028391">
        <property type="entry name" value="PLC-delta1_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015359">
        <property type="entry name" value="PLC_EF-hand-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000909">
        <property type="entry name" value="PLipase_C_PInositol-sp_X_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001711">
        <property type="entry name" value="PLipase_C_Pinositol-sp_Y"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10336:SF209">
        <property type="entry name" value="PHOSPHOINOSITIDE PHOSPHOLIPASE C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10336">
        <property type="entry name" value="PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE C FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09279">
        <property type="entry name" value="EF-hand_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00388">
        <property type="entry name" value="PI-PLC-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00387">
        <property type="entry name" value="PI-PLC-Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00390">
        <property type="entry name" value="PHPHLIPASEC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00148">
        <property type="entry name" value="PLCXc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00149">
        <property type="entry name" value="PLCYc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50007">
        <property type="entry name" value="PIPLC_X_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50008">
        <property type="entry name" value="PIPLC_Y_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 12" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 14" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="7 14" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <feature type="domain" description="EF-hand" evidence="18">
        <location>
            <begin position="136"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="18">
        <location>
            <begin position="172"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="PI-PLC Y-box" evidence="17">
        <location>
            <begin position="487"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="16">
        <location>
            <begin position="604"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="439"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="442"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="307"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="352"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="308"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="337"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="339"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="386"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="434"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="436"/>
        </location>
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                <person name="De Tomaso A."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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    <sequence length="861" mass="94893" checksum="6B3EE542F2D58410" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>F6YEQ8</accession>
    <name>F6YEQ8_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Eukaryotic translation initiation factor 2A</fullName>
            <shortName evidence="9">eIF-2A</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100175728</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 9">Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF-2 complex, it binds methionyl-tRNAi to 40S subunits in a codon-dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40S subunits in a GTP-dependent manner.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the WD repeat EIF2A family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="F6YEQ8"/>
    <dbReference type="FunCoup" id="F6YEQ8">
        <property type="interactions" value="890"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000005103"/>
    <dbReference type="Ensembl" id="ENSCINT00000005103.3">
        <property type="protein sequence ID" value="ENSCINP00000005103.3"/>
        <property type="gene ID" value="ENSCING00000002504.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00730000111053"/>
    <dbReference type="HOGENOM" id="CLU_013809_1_0_1"/>
    <dbReference type="InParanoid" id="F6YEQ8"/>
    <dbReference type="OMA" id="RCCAYSP"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001250">
        <property type="entry name" value="Eukaryotic translation initiation factor 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011387">
        <property type="entry name" value="TIF2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013979">
        <property type="entry name" value="TIF_beta_prop-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13227">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13227:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08662">
        <property type="entry name" value="eIF2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017222">
        <property type="entry name" value="eIF2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 9" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="8 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 9" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="5" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Translation initiation factor beta propellor-like" evidence="12">
        <location>
            <begin position="218"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="471"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="528"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="481"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="494"/>
            <end position="504"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR017222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF08662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000005103.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="583" mass="65610" checksum="1706BA3D93C53F28" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>F6YKT9</accession>
    <name>F6YKT9_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="22">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">wild type</strain>
        </source>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>eicosanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-eicosenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:47236"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57380"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:74691"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:47237"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>octadecanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-octadecenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:47240"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57394"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:71412"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:47241"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>oxidized [electron-transfer flavoprotein] + hexadecanoyl-CoA + H(+) = (2E)-hexadecenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:43448"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:61526"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:43449"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>tetradecanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-tetradecenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:47316"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57385"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:61405"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:47317"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 17">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 17">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001133">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6YKT9"/>
    <dbReference type="FunCoup" id="F6YKT9">
        <property type="interactions" value="152"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000003052"/>
    <dbReference type="Ensembl" id="ENSCINT00000003052.3">
        <property type="protein sequence ID" value="ENSCINP00000003052.3"/>
        <property type="gene ID" value="ENSCING00000001543.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157312"/>
    <dbReference type="HOGENOM" id="CLU_018204_11_2_1"/>
    <dbReference type="InParanoid" id="F6YKT9"/>
    <dbReference type="OMA" id="CDLANDW"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003995">
        <property type="term" value="F:acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.10:FF:000008">
        <property type="entry name" value="acyl-CoA dehydrogenase family member 9, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.110.10:FF:000006">
        <property type="entry name" value="very long-chain specific acyl-CoA dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.540.10:FF:000001">
        <property type="entry name" value="Very long-chain-specific acyl-CoA dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049448">
        <property type="entry name" value="ACAD9/ACADV-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006089">
        <property type="entry name" value="Acyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884">
        <property type="entry name" value="ACYL-COA DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884:SF9">
        <property type="entry name" value="COMPLEX I ASSEMBLY FACTOR ACAD9, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21343">
        <property type="entry name" value="ACAD9-ACADV_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00072">
        <property type="entry name" value="ACYL_COA_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00073">
        <property type="entry name" value="ACYL_COA_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0007">Acetylation</keyword>
    <keyword evidence="7 17" id="KW-0274">FAD</keyword>
    <keyword evidence="5 17" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="10 17" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase N-terminal" evidence="20">
        <location>
            <begin position="74"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="19">
        <location>
            <begin position="185"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="18">
        <location>
            <begin position="298"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="domain" description="ACAD9/ACADV-like C-terminal" evidence="21">
        <location>
            <begin position="505"/>
            <end position="622"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF21343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000003052.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="633" mass="70762" checksum="C946718016BD6D05" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F6YPP1</accession>
    <name>F6YPP1_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">chitinase</fullName>
            <ecNumber evidence="2">3.2.1.14</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1-&gt;4)-beta-linkages in chitin and chitodextrins.</text>
            <dbReference type="EC" id="3.2.1.14"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.2.1.14" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="F6YPP1"/>
    <dbReference type="STRING" id="7719.ENSCINP00000028293"/>
    <dbReference type="Ensembl" id="ENSCINT00000028539.1">
        <property type="protein sequence ID" value="ENSCINP00000028293.1"/>
        <property type="gene ID" value="ENSCING00000016282.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166041"/>
    <dbReference type="HOGENOM" id="CLU_1340202_0_0_1"/>
    <dbReference type="InParanoid" id="F6YPP1"/>
    <dbReference type="OMA" id="FENPNDC"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008843">
        <property type="term" value="F:endochitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.140.10">
        <property type="entry name" value="Chitin binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002557">
        <property type="entry name" value="Chitin-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036508">
        <property type="entry name" value="Chitin-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051940">
        <property type="entry name" value="Chitin_bind-dev_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23301">
        <property type="entry name" value="CHITIN BINDING PERITROPHIN-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23301:SF0">
        <property type="entry name" value="CHITIN-BINDING TYPE-2 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01607">
        <property type="entry name" value="CBM_14"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00494">
        <property type="entry name" value="ChtBD2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57625">
        <property type="entry name" value="Invertebrate chitin-binding proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50940">
        <property type="entry name" value="CHIT_BIND_II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="6" id="KW-0146">Chitin degradation</keyword>
    <keyword evidence="3" id="KW-0147">Chitin-binding</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003351545" description="chitinase" evidence="10">
        <location>
            <begin position="19"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="11">
        <location>
            <begin position="28"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-2" evidence="11">
        <location>
            <begin position="102"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="186"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000028293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="205" mass="21579" checksum="935FC379BDFD8CB9" modified="2011-07-27" version="1">MKLTVIITLCALTTCALGKPVDKKAELNSKCFEAGAPISSKPFEKPGDCDHFYQCSNGYLYTMPCAPGTAFSPALSVCDHPENVDGCGGSASTPAPPTKPADKECLDSDGNAITKGPFANPDDCALFYQCVAGQLYTLGCPPDLVYNPALSYCDYPKNVPSCGGVAPTSAPETTTKPFDDECVDANNQPTSTGPFENPNDCNSFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>F6YX11</accession>
    <name>F6YX11_CIOIN</name>
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        <recommendedName>
            <fullName evidence="5">Protein cereblon</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Protein ohgata</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">wild type</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Substrate recognition component of a DCX (DDB1-CUL4-X-box) E3 protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Has an essential role in mediating growth by negatively regulating insulin signaling. It also has a role in maintaining presynaptic function in the neuromuscular junction synapses of third-instar larvae.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Likely a component of a DCX (DDB1-CUL4-X-box) protein ligase complex. May interact with pic/DDB1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CRBN family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002581">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6YX11"/>
    <dbReference type="FunCoup" id="F6YX11">
        <property type="interactions" value="212"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000024553"/>
    <dbReference type="Ensembl" id="ENSCINT00000024799.2">
        <property type="protein sequence ID" value="ENSCINP00000024553.2"/>
        <property type="gene ID" value="ENSCING00000004842.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000016404"/>
    <dbReference type="HOGENOM" id="CLU_025648_1_1_1"/>
    <dbReference type="InParanoid" id="F6YX11"/>
    <dbReference type="OMA" id="SPQYIAR"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031464">
        <property type="term" value="C:Cul4A-RING E3 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030177">
        <property type="term" value="P:positive regulation of Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd15777">
        <property type="entry name" value="CRBN_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.150.20:FF:000005">
        <property type="entry name" value="Blast:Protein cereblon homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.40">
        <property type="entry name" value="LON domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.20">
        <property type="entry name" value="Peptide methionine sulfoxide reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034750">
        <property type="entry name" value="CULT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003111">
        <property type="entry name" value="Lon_prtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046336">
        <property type="entry name" value="Lon_prtase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004910">
        <property type="entry name" value="Yippee/Mis18/Cereblon"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14255">
        <property type="entry name" value="CEREBLON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14255:SF4">
        <property type="entry name" value="PROTEIN CEREBLON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02190">
        <property type="entry name" value="LON_substr_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03226">
        <property type="entry name" value="Yippee-Mis18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00464">
        <property type="entry name" value="LON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51788">
        <property type="entry name" value="CULT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51787">
        <property type="entry name" value="LON_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Lon N-terminal" evidence="15">
        <location>
            <begin position="69"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="CULT" evidence="16">
        <location>
            <begin position="313"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="30"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00046075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00046796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000024553.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="435" mass="49963" checksum="E61A81810304EFCD" modified="2012-04-18" version="2">VDDEQNELESSEEEEEGDSDPEDDDETENIKTPTNITFDKNLPTSHSYLGEMEDCSGVPYQKEDSYITMPIMYVNDFVLIPGQTLPLQIARFNEVALIQRVMEQEDKTFGVLTANPTISSGTQVTKNLYDFGCTAEIRSFRETDDHEVTQLRIVAVGRQRFQLMEKRTQLDGVKEANITVPTPVPLKVLSLTLNTLILPSKQSKEYNTSSSQFPLQSNYQHTWKHMRRNAWSTSWQPWVHRMYDVDFLRLEIFKELREWNGRLLETQCPVGATDFSFWVVTMVPLSVERRLFLLQLQSAVQRLRCELNLLRKSTTLHCSHCSLRIAERKDVFSMSTSGPMAAYVNPGGVVHETLTLYKTTSLDHVGRRSTEHSWFPGYAWTICECRRCSRHMGWRFTATNPNLIPQKFWGLTRSALSPQVADSDVIGDDQQTLCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>F6Z6C7</accession>
    <name>F6Z6C7_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
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                <person name="Inaba K."/>
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                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
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                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
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                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
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                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
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                <person name="Sasaki A."/>
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                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
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            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
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        </citation>
        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
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<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
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            <taxon>Ciona</taxon>
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    <reference evidence="13" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
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                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
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                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
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                <person name="Richardson P."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
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                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
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                <person name="Wasserscheid J."/>
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                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="12">wild type</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="12" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <text>a phosphate monoester + H2O = an alcohol + phosphate</text>
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            <name>Mg(2+)</name>
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        <text evidence="9">Binds 1 Mg(2+) ion.</text>
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    <comment type="cofactor">
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            <name>Zn(2+)</name>
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        <text evidence="9">Binds 2 Zn(2+) ions.</text>
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        </source>
    </evidence>
    <sequence length="202" mass="21821" checksum="B5595D5859001A16" modified="2012-04-18" version="2">MCGFVTISSARGWTDEEETTEYWYKLGQEEIDIALERENLNKNVARNIILFLGDGMSVATITAGRILKGQLEGKSGEESTLAMDQFHFAGLSKTYSVDQQVSDSACTATAYLCGVKSDYSTIGLNGNVEYGDCSSVKGNEVESTLVKAYKAGKSTGIVTTTRIVHASPAGTYAHTPSRGWYGDNNLPESAIQEGCKDIAQQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>F7AM61</accession>
    <name>F7AM61_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Tumor suppressor candidate 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100184901</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.</text>
    </comment>
    <comment type="pathway">
        <text evidence="12">Protein modification.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the OST3/OST6 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="F7AM61"/>
    <dbReference type="FunCoup" id="F7AM61">
        <property type="interactions" value="74"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000003542"/>
    <dbReference type="Ensembl" id="ENSCINT00000003542.3">
        <property type="protein sequence ID" value="ENSCINP00000003542.3"/>
        <property type="gene ID" value="ENSCING00000001754.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000012030"/>
    <dbReference type="HOGENOM" id="CLU_052855_0_0_1"/>
    <dbReference type="InParanoid" id="F7AM61"/>
    <dbReference type="OMA" id="VLFGMYS"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008250">
        <property type="term" value="C:oligosaccharyltransferase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903830">
        <property type="term" value="P:magnesium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018279">
        <property type="term" value="P:protein N-linked glycosylation via asparagine"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000009">
        <property type="entry name" value="Tumor suppressor candidate 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021149">
        <property type="entry name" value="OligosaccharylTrfase_OST3/OST6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12692">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12692:SF0">
        <property type="entry name" value="GH11935P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04756">
        <property type="entry name" value="OST3_OST6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14" id="KW-0732">Signal</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003353447" evidence="14">
        <location>
            <begin position="26"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="181"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="212"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="266"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="296"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00043952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000003542.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="330" mass="37792" checksum="574D4AB2D49E67EB" modified="2012-04-18" version="2">KKDRIMVKYILVVTFLLAIFSLCSCKTKQDLLDEKVKQLTEWSNRRTVIQMNSNKYSTYVKSKPRNYSMIVMFTALDPKRGCSVCREANDEFTILANSYRFSPAYTSSLFFAVVDFDEGSEVFQSLGLNSAPAFMHFPPKGKRKGADTYDIQRLGFHADNLAKWMAERTEVVVRIFRPPNYTGTVVLVLLFSLAGGLLYVRRNSLEFFYNKTFWSILSIFIVLAMTSGQMWNHIRGPPYYHRNPQNGAIHYIHGSSQGQLVAETHIIMLIHAAIVGGFILLNEAHQPKVDISKRRIMAIGGLVLVAFFLSLLMSIFRQKYQGYPYSFLLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>F7ANX0</accession>
    <name>F7ANX0_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="F7ANX0"/>
    <dbReference type="STRING" id="7719.ENSCINP00000027263"/>
    <dbReference type="Ensembl" id="ENSCINT00000027509.2">
        <property type="protein sequence ID" value="ENSCINP00000027263.2"/>
        <property type="gene ID" value="ENSCING00000003180.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00910000144476"/>
    <dbReference type="HOGENOM" id="CLU_001265_30_11_1"/>
    <dbReference type="InParanoid" id="F7ANX0"/>
    <dbReference type="OMA" id="AYKIPFI"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055056">
        <property type="term" value="F:D-glucose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046323">
        <property type="term" value="P:D-glucose import"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070837">
        <property type="term" value="P:dehydroascorbic acid transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045263">
        <property type="entry name" value="GLUT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23503:SF8">
        <property type="entry name" value="FACILITATED GLUCOSE TRANSPORTER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23503">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="15"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="53"/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
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                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
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                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
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                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
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                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
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                <person name="Detter C."/>
                <person name="Doggett N."/>
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                <person name="Richardson P."/>
                <person name="Lucas S."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">wild type</strain>
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    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Putnam N."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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        <text evidence="7">In the cytoplasm, catalyzes the conversion of glucose-6-phosphate to fructose-6-phosphate, the second step in glycolysis, and the reverse reaction during gluconeogenesis. Besides it's role as a glycolytic enzyme, also acts as a secreted cytokine: acts as an angiogenic factor (AMF) that stimulates endothelial cell motility. Acts as a neurotrophic factor, neuroleukin, for spinal and sensory neurons. It is secreted by lectin-stimulated T-cells and induces immunoglobulin secretion.</text>
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        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035482">
        <property type="entry name" value="SIS_PGI_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11469">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11469:SF1">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00342">
        <property type="entry name" value="PGI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00662">
        <property type="entry name" value="G6PISOMERASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SIS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00174">
        <property type="entry name" value="P_GLUCOSE_ISOMERASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51463">
        <property type="entry name" value="P_GLUCOSE_ISOMERASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="5 8" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="6 8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00046209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000019213.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="232" mass="26053" checksum="2B249A7E27FF6B70" modified="2012-04-18" version="2">MLGVWYNNFHGAQSHALLPYDQYMHRFAAYFQQGDMESNGKYVTRSGHQVDYSTGPVVWGEPGTNGQHAFYQLIHQGTKIVPADFLASAKTHNPISDGKHHKILLSNFLAQTEALMIGKTSEQVKTELEKAGTSPEALQKIMPHKVFLGNRPTNSIVYPKLTPFMLGLLIAMYEHKIFTQGIIWDINSYDQWGVELGKQLAKIIEPELSCSDDVTSHDSSTNGLMKFLKSHM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>F7B1C7</accession>
    <name>F7B1C7_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">U2A'/phosphoprotein 32 family A C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01002101">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F7B1C7"/>
    <dbReference type="FunCoup" id="F7B1C7">
        <property type="interactions" value="15"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000012792"/>
    <dbReference type="Ensembl" id="ENSCINT00000012792.3">
        <property type="protein sequence ID" value="ENSCINP00000012792.3"/>
        <property type="gene ID" value="ENSCING00000006197.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159748"/>
    <dbReference type="HOGENOM" id="CLU_1351580_0_0_1"/>
    <dbReference type="InParanoid" id="F7B1C7"/>
    <dbReference type="OMA" id="YSANMKS"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050836">
        <property type="entry name" value="SDS22/Internalin_LRR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46652:SF8">
        <property type="entry name" value="LEUCINE RICH REPEAT CONTAINING 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46652">
        <property type="entry name" value="LEUCINE-RICH REPEAT AND IQ DOMAIN-CONTAINING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14580">
        <property type="entry name" value="LRR_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52075">
        <property type="entry name" value="Outer arm dynein light chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000012792.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="203" mass="23399" checksum="A18AFE5C310A3CDE" modified="2012-04-18" version="2">IMNLSGNKITSTEGVEHPLLEQLNLNSNLIPLVSGLNPKFLTNLTTLELRGNKLTTTAGIYLPNLKNLYLAGNSLKQVEDLDKLENLMILHLRDNQIDKLDGFTENMKKLQYINLRANNVSDLKELKKMVALPMLRALVLNDNPCLEEDGYRMEVLIVLRKLERLDKDEYTEDERVEAEDLYEQRRQEELMKSEEQEEIADEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>F7B1K2</accession>
    <name>F7B1K2_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="F7B1K2"/>
    <dbReference type="Ensembl" id="ENSCINT00000012789.3">
        <property type="protein sequence ID" value="ENSCINP00000012789.3"/>
        <property type="gene ID" value="ENSCING00000006195.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063555"/>
    <dbReference type="HOGENOM" id="CLU_075590_1_1_1"/>
    <dbReference type="InParanoid" id="F7B1K2"/>
    <dbReference type="OMA" id="TWNITGQ"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005614">
        <property type="term" value="C:interstitial matrix"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005520">
        <property type="term" value="F:insulin-like growth factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001558">
        <property type="term" value="P:regulation of cell growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00104">
        <property type="entry name" value="KAZAL_FS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000867">
        <property type="entry name" value="IGFBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011390">
        <property type="entry name" value="IGFBP_rP_mac25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002350">
        <property type="entry name" value="Kazal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036058">
        <property type="entry name" value="Kazal_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14186">
        <property type="entry name" value="INSULIN-LIKE GROWTH FACTOR BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14186:SF26">
        <property type="entry name" value="KAZAL TYPE SERINE PEPTIDASE INHIBITOR DOMAIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00219">
        <property type="entry name" value="IGFBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07648">
        <property type="entry name" value="Kazal_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00121">
        <property type="entry name" value="IB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00280">
        <property type="entry name" value="KAZAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100895">
        <property type="entry name" value="Kazal-type serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51323">
        <property type="entry name" value="IGFBP_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51465">
        <property type="entry name" value="KAZAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045664082" evidence="6">
        <location>
            <begin position="25"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="IGFBP N-terminal" evidence="8">
        <location>
            <begin position="26"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="Kazal-like" evidence="9">
        <location>
            <begin position="93"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="7">
        <location>
            <begin position="147"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000012789.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="250" mass="26991" checksum="6CEED20BB60CD665" modified="2025-10-08" version="3">MWGIRWIALSRNIRFFIIKHPLLFDWTTSCGVCDPEQCSATPTSCPGGLIRDACDCCYVCGNLEDTPCDLPGAMFRFGNCGEGLVCTPVEPDDVGLDASDSYCACAARDVICGSDGRTYSNPCRFRRASGRVAGLEIARQGPCRAAPQVEIPPMNQTAESGSSAVFSCSVSGFPLAWFEWTLNGESVFQPNLDDGVTVQVRSGPLPYQVTTWLEIDRVMTSHAGLFTCIAKNEFGISNATARLNVVAGNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>F7B8Q9</accession>
    <name>F7B8Q9_CIOIN</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Tunicata</taxon>
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            <taxon>Phlebobranchia</taxon>
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            <taxon>Ciona</taxon>
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    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="F7B8Q9"/>
    <dbReference type="Ensembl" id="ENSCINT00000027452.1">
        <property type="protein sequence ID" value="ENSCINP00000027206.1"/>
        <property type="gene ID" value="ENSCING00000015294.1"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1558751_0_0_1"/>
    <dbReference type="InParanoid" id="F7B8Q9"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000027206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="172" mass="19640" checksum="44E28B7335DE7A9D" modified="2011-07-27" version="1">MELSAQEAAEVVNHLLRSEPNNGKPVTSQEAMEIIRHVRDKSYGEERPPMDREKSIVLHPHEVANIFNKFRNRPTVTESDEPIRNEGGIASSYDPSDPGDRAPNRINREFRECSPIQEESSGGKHGNPRVAWTPEADHEEPVVKRVRKKPKRFLCESSIDPDQKVHDWLEST</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>F7BHW5</accession>
    <name>F7BHW5_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transcription initiation factor IIE subunit beta</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">wild type</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6 7">Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Tetramer of two alpha and two beta chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the TFIIE beta subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001172">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="FunCoup" id="F7BHW5">
        <property type="interactions" value="677"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000026391"/>
    <dbReference type="Ensembl" id="ENSCINT00000026637.2">
        <property type="protein sequence ID" value="ENSCINP00000026391.2"/>
        <property type="gene ID" value="ENSCING00000014661.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000011749"/>
    <dbReference type="HOGENOM" id="CLU_086770_0_0_1"/>
    <dbReference type="InParanoid" id="F7BHW5"/>
    <dbReference type="OMA" id="AFKRRAM"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005673">
        <property type="term" value="C:transcription factor TFIIE complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07977">
        <property type="entry name" value="TFIIE_beta_winged_helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000177">
        <property type="entry name" value="Transcription initiation factor IIE subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040501">
        <property type="entry name" value="TFA2_Winged_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016656">
        <property type="entry name" value="TFIIE-bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003166">
        <property type="entry name" value="TFIIE_bsu_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12716:SF8">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIE SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12716">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIE, BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18121">
        <property type="entry name" value="TFA2_Winged_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02186">
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                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
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                <person name="Satake M."/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
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                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
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                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
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                <person name="Awazu S."/>
                <person name="Azumi K."/>
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                <person name="Kobayashi K."/>
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                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
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                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
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                <person name="Nomoto H."/>
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                <person name="Rigoutsos I."/>
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                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
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                <person name="Kohara Y."/>
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            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
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            <authorList>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Gelpke M."/>
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                <person name="Hastings K.E."/>
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                <person name="Putnam N."/>
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                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
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                <person name="Hayashi H."/>
                <person name="Hino K."/>
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                <person name="Kobayashi M."/>
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                <person name="Makabe K.W."/>
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                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
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            <authorList>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        </subcellularLocation>
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    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
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                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
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                <person name="Kobayashi K."/>
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                <person name="Manohar C."/>
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                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="54" mass="6411" checksum="F356976F2D2BC608" modified="2012-04-18" version="1">ISRSRPYKNILAFHSSQHTFVNKFEVQAVNKYKLFDIIFLAPNNTGINFYRFSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>H2XQR3</accession>
    <name>H2XQR3_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Guanine nucleotide-binding protein subunit gamma</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">G proteins are composed of 3 units; alpha, beta and gamma.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cell membrane</location>
            <topology evidence="1 9">Lipid-anchor</topology>
            <orientation evidence="1 9">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the G protein gamma family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="H2XQR3"/>
    <dbReference type="FunCoup" id="H2XQR3">
        <property type="interactions" value="52"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000031997"/>
    <dbReference type="Ensembl" id="ENSCINT00000036693.1">
        <property type="protein sequence ID" value="ENSCINP00000031997.1"/>
        <property type="gene ID" value="ENSCING00000020287.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01100000263497"/>
    <dbReference type="HOGENOM" id="CLU_168377_3_1_1"/>
    <dbReference type="InParanoid" id="H2XQR3"/>
    <dbReference type="OMA" id="LKLEVHI"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005834">
        <property type="term" value="C:heterotrimeric G-protein complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031681">
        <property type="term" value="F:G-protein beta-subunit binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00068">
        <property type="entry name" value="GGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.260.10:FF:000001">
        <property type="entry name" value="Guanine nucleotide-binding protein subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.260.10">
        <property type="entry name" value="Transducin (heterotrimeric G protein), gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001770">
        <property type="entry name" value="G-protein_gamma"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015898">
        <property type="entry name" value="G-protein_gamma-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036284">
        <property type="entry name" value="GGL_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13809">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN GAMMA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00631">
        <property type="entry name" value="G-gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00321">
        <property type="entry name" value="GPROTEING"/>
    </dbReference>
    <dbReference type="SMART" id="SM01224">
        <property type="entry name" value="G_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00224">
        <property type="entry name" value="GGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48670">
        <property type="entry name" value="Transducin (heterotrimeric G protein), gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50058">
        <property type="entry name" value="G_PROTEIN_GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="8" id="KW-0636">Prenylation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0807">Transducer</keyword>
    <feature type="domain" description="G protein gamma" evidence="10">
        <location>
            <begin position="2"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000031997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="67" mass="7940" checksum="347F68CAC4FA9C75" modified="2012-04-18" version="1">MATRSVRELEKQIEELRYEANTERVKVSQSCKELMDYCESHASQDYFLYKTDKTNPFKESRSPCVVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>H2XQY6</accession>
    <name>H2XQY6_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">LRRNT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01002980">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XQY6"/>
    <dbReference type="STRING" id="7719.ENSCINP00000032070"/>
    <dbReference type="Ensembl" id="ENSCINT00000037019.1">
        <property type="protein sequence ID" value="ENSCINP00000032070.1"/>
        <property type="gene ID" value="ENSCING00000022633.1"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1531992_0_0_1"/>
    <dbReference type="InParanoid" id="H2XQY6"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24366">
        <property type="entry name" value="IG(IMMUNOGLOBULIN) AND LRR(LEUCINE RICH REPEAT) DOMAINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24366:SF96">
        <property type="entry name" value="LEUCINE RICH REPEAT CONTAINING 53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000032070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="175" mass="19955" checksum="7825A57C025A58CC" modified="2012-04-18" version="1">MDQIIGYFSRPMSTQNVYQDLYYSNSYTDETNTIQNNTSPEQNLNSGEESLKTLDLGQLGLSETPGTLPGSLVTLWLDGNRIERISKEAFSRTKQLNILHLARNGLYEIEQMAFRDLNKLSIFDLSNNRLANLSSDVFYGLGMLVRLRLHKNRQLTELPEKVRPKPIESACLEPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>H2XRS9</accession>
    <name>H2XRS9_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein N-terminal asparagine amidohydrolase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Ciona intestinalis</name>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
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            <taxon>Ciona</taxon>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    <sequence length="350" mass="39544" checksum="782BC30F9AA5F632" modified="2012-04-18" version="1">MQSYFMCFLLITSRLFVISCFRLLMFNMPLIINGIEIKDSDRILDDLQLFVQKYKNVYKDCSSRLKSTPVTLVTRCQCCLYVMQGELATVGGADKSIKCVGTDMATTCHIVVIRCRQTATTSLAHFDGSFLVDGAKEMLKSIENIRTPEGAQGYDLDLHIIGGFDDDRKLSGKLSTDLLRTFQTLSSDRGLKLYLRTFCCSPFNTCVVTKHGVEINSPVIFGVVVTVGDGKIYGADFAEDARGPVRAMRAARGVHGRKQMFNVYDYVKERFIVPPFTYQPLPFIEEWIEQSDSFIKENMSTSPDVEPAFFAPDTRESLRFIATHPNPMHSIFKSNLPLFYKLDGGLWKQE</sequence>
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            <fullName evidence="4">PDZ domain-containing protein</fullName>
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    <organism evidence="5 6">
        <name type="scientific">Ciona intestinalis</name>
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        <lineage>
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    <reference evidence="6" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
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                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
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                <person name="Kobayashi K."/>
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                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">wild type</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="Ensembl" id="ENSCINT00000031153.1">
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        <property type="term" value="C:apical plasma membrane"/>
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        <source>
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        <source>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
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                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
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                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Putnam N."/>
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                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
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                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
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                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="De Tomaso A."/>
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                <person name="Hastings K.E."/>
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                <person name="Wang H.G."/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
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                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
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                <person name="Huang W."/>
                <person name="Kawashima T."/>
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                <person name="Meinertzhagen I.A."/>
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                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
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</entry>
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            <fullName evidence="10">Transporter</fullName>
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        <name evidence="12" type="primary">LOC100187411</name>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
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                <person name="Miura S."/>
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                <person name="Nomoto H."/>
                <person name="Ohta F."/>
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                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
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                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">wild type</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Golgi apparatus</location>
            <location evidence="11">Golgi stack membrane</location>
            <topology evidence="11">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the glycosyltransferase 10 family.</text>
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    <dbReference type="EC" id="2.4.1.-" evidence="11"/>
    <dbReference type="EMBL" id="EAAA01001388">
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:alpha-(1-&gt;3)-fucosyltransferase activity"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11660">
        <property type="entry name" value="Glycosyl transferase family 10, C-terminal domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Glyco_tran_10_C"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR001503">
        <property type="entry name" value="Glyco_trans_10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038577">
        <property type="entry name" value="GT10-like_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11929">
        <property type="entry name" value="ALPHA- 1,3 -FUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11929:SF145">
        <property type="entry name" value="ALPHA-(1,3)-FUCOSYLTRANSFERASE FUT-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17039">
        <property type="entry name" value="Glyco_tran_10_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00852">
        <property type="entry name" value="Glyco_transf_10"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="4 11" id="KW-0328">Glycosyltransferase</keyword>
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    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
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            <begin position="49"/>
            <end position="155"/>
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        <location>
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            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00008919"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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            <dbReference type="Pfam" id="PF17039"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000033558.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    </evidence>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">wild type</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01002439">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XV78"/>
    <dbReference type="Ensembl" id="ENSCINT00000032326.1">
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        <property type="gene ID" value="ENSCING00000019067.1"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3241857_0_0_1"/>
    <dbReference type="InParanoid" id="H2XV78"/>
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        <property type="component" value="Chromosome 7"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000033562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="43" mass="5227" checksum="2AF7F016F22CF396" modified="2012-04-18" version="1">MRIWLCDSLHILCLLPNGQKKNKNMSRLFPTYCITKINFFDYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>H2XVQ7</accession>
    <name>H2XVQ7_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">WAPL domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
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            <taxon>Metazoa</taxon>
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            <taxon>Ciona</taxon>
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    <reference evidence="5" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">wild type</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WAPL family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001039">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XVQ7"/>
    <dbReference type="STRING" id="7719.ENSCINP00000033741"/>
    <dbReference type="Ensembl" id="ENSCINT00000035060.1">
        <property type="protein sequence ID" value="ENSCINP00000033741.1"/>
        <property type="gene ID" value="ENSCING00000018718.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015768"/>
    <dbReference type="HOGENOM" id="CLU_1389776_0_0_1"/>
    <dbReference type="InParanoid" id="H2XVQ7"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039874">
        <property type="entry name" value="WAPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022771">
        <property type="entry name" value="WAPL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012502">
        <property type="entry name" value="WAPL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22100">
        <property type="entry name" value="WINGS APART-LIKE PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22100:SF13">
        <property type="entry name" value="WINGS APART-LIKE PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07814">
        <property type="entry name" value="WAPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51271">
        <property type="entry name" value="WAPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WAPL" evidence="3">
        <location>
            <begin position="1"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="28"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000033741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="196" mass="22537" checksum="819E900F29954133" modified="2012-04-18" version="1">MLSRDRRMEIDKPTLQLLLSLLDPLDRESNNAGQETPETNKEKSATKYRFFKSKDKSSQLEEEARNMRSVEQKILTMVKGCSDNLINENVVSVKRLASEALIRFTARQTPEWYREEVRLKGGLDHIISLVKQHVDHVTELSGSAPVSIDASFPQDRPLCMLERLLKVLENTVMHSHRNAMYLVTHLRALLLKKIAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>H2XVS7</accession>
    <name>H2XVS7_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">wild type</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01001993">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XVS7"/>
    <dbReference type="Ensembl" id="ENSCINT00000035058.1">
        <property type="protein sequence ID" value="ENSCINP00000033761.1"/>
        <property type="gene ID" value="ENSCING00000020682.1"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3224242_0_0_1"/>
    <dbReference type="InParanoid" id="H2XVS7"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000033761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="44" mass="4904" checksum="A320AFB5A9C64628" modified="2012-04-18" version="1">MSQSLSQVITLSPYLTYKNDCKICHKDICKLITSTGFLSPVNLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>H2XVZ6</accession>
    <name>H2XVZ6_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA-directed primase/polymerase protein</fullName>
            <ecNumber evidence="6">2.7.7.102</ecNumber>
            <ecNumber evidence="2">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:22509"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>ssDNA + n NTP = ssDNA/pppN(pN)n-1 hybrid + (n-1) diphosphate.</text>
            <dbReference type="EC" id="2.7.7.102"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic-type primase small subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.102" evidence="6"/>
    <dbReference type="EC" id="2.7.7.7" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="H2XVZ6"/>
    <dbReference type="FunCoup" id="H2XVZ6">
        <property type="interactions" value="20"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000033830"/>
    <dbReference type="Ensembl" id="ENSCINT00000034532.1">
        <property type="protein sequence ID" value="ENSCINP00000033830.1"/>
        <property type="gene ID" value="ENSCING00000023015.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000003901"/>
    <dbReference type="HOGENOM" id="CLU_027838_0_0_1"/>
    <dbReference type="InParanoid" id="H2XVZ6"/>
    <dbReference type="OMA" id="HYEVIQD"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042276">
        <property type="term" value="P:error-prone translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006264">
        <property type="term" value="P:mitochondrial DNA replication"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
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        <source>
            <dbReference type="ARBA" id="ARBA00009762"/>
        </source>
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        <source>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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            <dbReference type="ARBA" id="ARBA00047303"/>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
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    </evidence>
    <sequence length="436" mass="51102" checksum="30427E24B833DBE6" modified="2012-04-18" version="1">VKTKRKYCFTKIETQCKKFQSTPMVSMYKPRLCEFELPAPTWKLFTKQNGAIEYAKCANERLFVFGNEFGDGGIRNFVAATIEEFWFYYSQKSFGERHFYEVIQENAHCHLYFDIEFNKTMNPEKNGGNMISIWIAYVCSCLLRSFCLKLTRKCFVELESSSDVKYSQHVVVHMPQGCVFKDNIHAGRFVKKICRDLKEYLSLEPKTAPSDILWIEELEFRDKLQDLVVKTTNGNGIFIDEGVYTRNRNFRLFMSRKRNKNFELKISDKFFKPIQQKLGENENSFKLKQIFFVSIVTNVCEDKVVRFLEVPTEKDEKQANNLSRNDAIVAPVTTYGLESSEFGIPTKEISEFITNYIKRFNEKARIRKLTYFPTTRVINYDITGTRFCHNIGREHRNNNIMYVVDVTGRSCHQKCYDSDCRAIGYKSPSFSLPQEI</sequence>
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    <accession>H2XWB6</accession>
    <name>H2XWB6_CIOIN</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
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    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
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            <taxon>Ciona</taxon>
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    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="entry name" value="CCDC150"/>
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        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 150"/>
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        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 150"/>
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    </dbReference>
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        </location>
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        <location>
            <begin position="201"/>
            <end position="220"/>
        </location>
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        <location>
            <begin position="207"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000033950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="341" mass="39189" checksum="9DF6677AE73FAB04" modified="2012-04-18" version="1">MAQIEVTKDQEKSRVSQMKRELSDCRSDNARLSSTIDDITTRQAQLQEVLDQVQTKLGRKDGQLAALTDEKSQLEHQVHILNTEIAKLESTSHNRDALEQSRITPALEALERARHDNVRLAASLDDVLRTNAQLKDDLDKCKTDLEIQRERNETILRQQKSSEEEALLTSRSNNDRLQQMKDQFNRERSAIKRQLTTQVNELKKTAESAQSRNTQLTRGNSELRSKLADREQTIAKQKEKMKQQKISLDRMQAERKSKIFTNQRIKEIDSELGELEKTKQSYIEKHKEQASIIATFNTEVSSLQEDIAALSHAQKLSRETSSKLESELGKEKAEKEVLINK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>H2XWG7</accession>
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        <recommendedName>
            <fullName evidence="5">U3 small nucleolar RNA-associated protein 11</fullName>
            <shortName evidence="5">U3 snoRNA-associated protein 11</shortName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
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    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
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                <person name="Wiley G.B."/>
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                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">wild type</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="7" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="5">Involved in nucleolar processing of pre-18S ribosomal RNA.</text>
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    <comment type="subunit">
        <text evidence="5">Component of the ribosomal small subunit (SSU) processome.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
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            <location evidence="1 5">Nucleolus</location>
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    <comment type="similarity">
        <text evidence="2 5">Belongs to the UTP11 family.</text>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR12838:SF0">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 11-RELATED"/>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
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                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
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                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
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                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
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                <person name="Tassy O."/>
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                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
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                <person name="Richardson P."/>
                <person name="Lucas S."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
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                <consortium name="Ensembl"/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
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                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
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                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
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                <person name="Ueno K."/>
                <person name="Yamada L."/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Putnam N."/>
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                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
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                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
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                <person name="Wiley G.B."/>
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                <person name="Roe B.A."/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
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                <person name="Chapman J."/>
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                <person name="De Tomaso A."/>
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                <person name="Di Gregorio A."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
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                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
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                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
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                <person name="Huang W."/>
                <person name="Kawashima T."/>
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                <person name="Wang H.G."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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        <text evidence="10">Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient.</text>
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</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>H2Y3B8</accession>
    <name>H2Y3B8_CIOIN</name>
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        <recommendedName>
            <fullName evidence="3">SS18 N-terminal domain-containing protein</fullName>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">wild type</strain>
        </source>
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    <reference evidence="4" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <text evidence="1">Belongs to the SS18 family.</text>
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</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>H2Y3U0</accession>
    <name>H2Y3U0_CIOIN</name>
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            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="Ensembl" id="ENSCINT00000033286.1">
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        <property type="gene ID" value="ENSCING00000023662.1"/>
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    <dbReference type="HOGENOM" id="CLU_1656072_0_0_1"/>
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    <proteinExistence type="predicted"/>
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    <feature type="region of interest" description="Disordered" evidence="1">
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            <begin position="129"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="132"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000036576.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    </evidence>
    <sequence length="160" mass="18090" checksum="F0E98B59CB89CFBD" modified="2012-04-18" version="1">MPGNREEEVSLRSHSPIAIQAWPQRKIYLSTQPHMTQSLTSYTEQRNYNTSTTINDLKTARSSTQQTKRVHKPLTHRLSFAKSYTNQISAINCHNSDRGYRIPVSHRRHVIKEPVNGTDTQVTTIPAHISDLNKSSTKVSGQDNNSTSNQLHTSLPSKQN</sequence>
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<entry dataset="TrEMBL" created="2012-09-05" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>I6L237</accession>
    <name>I6L237_CIOIN</name>
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            <fullName evidence="8">ATP-dependent (S)-NAD(P)H-hydrate dehydratase</fullName>
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        <name evidence="10" type="primary">LOC100183233</name>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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            <taxon>Eukaryota</taxon>
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    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
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                <person name="Miura S."/>
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                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">wild type</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ATP, which is converted to ADP. Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>(6S)-NADHX + ATP = ADP + phosphate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19017"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:64074"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.2.1.93"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>(6S)-NADPHX + ATP = ADP + phosphate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32231"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.2.1.93"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the NnrD/CARKD family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.93" evidence="8"/>
    <dbReference type="EMBL" id="EAAA01001543">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="I6L237"/>
    <dbReference type="FunCoup" id="I6L237">
        <property type="interactions" value="55"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSCINT00000006335.3">
        <property type="protein sequence ID" value="ENSCINP00000006335.3"/>
        <property type="gene ID" value="ENSCING00000003131.3"/>
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    <dbReference type="GeneTree" id="ENSGT00390000000917"/>
    <dbReference type="HOGENOM" id="CLU_030651_3_0_1"/>
    <dbReference type="InParanoid" id="I6L237"/>
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    <dbReference type="GO" id="GO:0005524">
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        <property type="project" value="GO_Central"/>
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        <property type="term" value="P:nicotinamide nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Ribokinase-like"/>
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        <property type="entry name" value="yjeF_cterm"/>
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        <property type="entry name" value="Carb_kinase"/>
        <property type="match status" value="1"/>
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    <keyword evidence="1 8" id="KW-0597">Phosphoprotein</keyword>
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        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:64076"/>
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        <ligand>
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            <title>Systematic analysis of embryonic expression profiles of zinc finger genes in Ciona intestinalis.</title>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
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                <person name="Hastings K.E."/>
                <person name="Ho I."/>
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                <person name="Huang W."/>
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                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Putnam N."/>
                <person name="Rash S."/>
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                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
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                <person name="Yoshizaki F."/>
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                <person name="Zhang C."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
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        <citation type="journal article" date="2009" name="Biol. Cell" volume="101" first="91" last="103">
            <title>A novel neuronal calcium sensor family protein, calaxin, is a potential Ca(2+)-dependent regulator for the outer arm dynein of metazoan cilia and flagella.</title>
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                <person name="Padma P."/>
                <person name="Konno A."/>
                <person name="Satouh Y."/>
                <person name="Ogawa K."/>
                <person name="Inaba K."/>
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            <title>Crystal structure of a Ca&lt;sup&gt;2+&lt;/sup&gt;-dependent regulator of flagellar motility reveals the open-closed structural transition.</title>
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                <person name="Shojima T."/>
                <person name="Hou F."/>
                <person name="Takahashi Y."/>
                <person name="Matsumura Y."/>
                <person name="Okai M."/>
                <person name="Nakamura A."/>
                <person name="Mizuno K."/>
                <person name="Inaba K."/>
                <person name="Kojima M."/>
                <person name="Miyakawa T."/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
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                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
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                <person name="Oishi K."/>
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                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
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                <person name="Lindquist E."/>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01002295">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="A0A1W3JI05"/>
    <dbReference type="STRING" id="7719.ENSCINP00000009254"/>
    <dbReference type="Ensembl" id="ENSCINT00000009254.3">
        <property type="protein sequence ID" value="ENSCINP00000009254.3"/>
        <property type="gene ID" value="ENSCING00000004476.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100181370"/>
    <dbReference type="KEGG" id="cin:100181370"/>
    <dbReference type="eggNOG" id="KOG2218">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000017006"/>
    <dbReference type="InParanoid" id="A0A1W3JI05"/>
    <dbReference type="OMA" id="KRRVEWT"/>
    <dbReference type="OrthoDB" id="2189254at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070939">
        <property type="term" value="C:Dsl1/NZR complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.670">
        <property type="entry name" value="Dsl1p vesicle tethering complex, Tip20p subunit, domain D"/>
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        <property type="entry name" value="EXOC6PINT-1/Sec15/Tip20_C_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007528">
        <property type="entry name" value="RINT1_Tip20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13520:SF0">
        <property type="entry name" value="RAD50-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13520">
        <property type="entry name" value="RAD50-INTERACTING PROTEIN 1 RINT-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04437">
        <property type="entry name" value="RINT1_TIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51386">
        <property type="entry name" value="RINT1_TIP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000009254.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="173" mass="19791" checksum="5E5551827B336FDD" modified="2017-06-07" version="1">MYDGWLALPTAEEQLVFVVSQSACGMLAVLQDRLHFLEQRLCRELFTQLWRVIAENVDIYLFNEVIVKNHFNSGGAAQIHYDMTRNLFPMFGHYTSKPDNYFKRVKEGCILLTLQSGSALLLREVLEESLKPPDPMDPHPTPVKPTSALNDIGVFLLSAKQALDIIKRRVEWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>F6PHP1</accession>
    <name>F6PHP1_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Adenine DNA glycosylase</fullName>
            <ecNumber evidence="4">3.2.2.31</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolyzes free adenine bases from 7,8-dihydro-8-oxoguanine:adenine mismatched double-stranded DNA, leaving an apurinic site.</text>
            <dbReference type="EC" id="3.2.2.31"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Nth/MutY family.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.31" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="F6PHP1"/>
    <dbReference type="STRING" id="7719.ENSCINP00000006291"/>
    <dbReference type="Ensembl" id="ENSCINT00000006291.3">
        <property type="protein sequence ID" value="ENSCINP00000006291.3"/>
        <property type="gene ID" value="ENSCING00000003105.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00510000047220"/>
    <dbReference type="HOGENOM" id="CLU_012862_2_1_1"/>
    <dbReference type="InParanoid" id="F6PHP1"/>
    <dbReference type="OMA" id="FERWIAK"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000701">
        <property type="term" value="F:purine-specific mismatch base pair DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.30:FF:000002">
        <property type="entry name" value="Adenine DNA glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1670.10">
        <property type="entry name" value="Helix-hairpin-Helix base-excision DNA repair enzymes (C-terminal)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.30">
        <property type="entry name" value="Hypothetical protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023170">
        <property type="entry name" value="HhH_base_excis_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044298">
        <property type="entry name" value="MIG/MutY"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42944">
        <property type="entry name" value="ADENINE DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42944:SF1">
        <property type="entry name" value="ADENINE DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00730">
        <property type="entry name" value="HhH-GPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="DNA-glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12" id="KW-0234">DNA repair</keyword>
    <keyword evidence="13" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="11" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HhH-GPD" evidence="14">
        <location>
            <begin position="54"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000006291.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="170" mass="19302" checksum="AC06EC959BBDCB7C" modified="2012-04-18" version="2">MSSYVFTENEIEVLRKLLLTWYAGNKRDLPWRRMADSCTTLSQHAYAVWVSEVMLQQTRVATVVEYYNRWMQKWPDIQSLSNSTEEEVNELWSGLGYYSRGRRMRQAAIKLVGEYDGKFPETSVDLLKTMPGVGRYTASAIASMAFKEVTGVVDGNVGSGCCVGFRAIGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>F6PNW9</accession>
    <name>F6PNW9_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Acyl-CoA dehydrogenase family member 11</fullName>
        </recommendedName>
    </protein>
    <organism evidence="26 27">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26">wild type</strain>
        </source>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Acyl-CoA dehydrogenase, that exhibits maximal activity towards saturated C22-CoA. Probably participates in beta-oxydation and energy production but could also play a role in the metabolism of specific fatty acids to control fatty acids composition of cellular lipids in brain.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a 2,3-saturated acyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = a (2E)-enoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:44704"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:58856"/>
            <dbReference type="ChEBI" id="CHEBI:65111"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:44705"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>docosanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-docosenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:47228"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:65059"/>
            <dbReference type="ChEBI" id="CHEBI:74692"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:47229"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>eicosanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-eicosenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:47236"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57380"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:74691"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:47237"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>hexacosanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-hexacosenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:48216"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:64868"/>
            <dbReference type="ChEBI" id="CHEBI:74281"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:48217"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>tetracosanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-tetracosenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:47232"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:65052"/>
            <dbReference type="ChEBI" id="CHEBI:74693"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:47233"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>tricosanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-tricosenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:48220"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:90118"/>
            <dbReference type="ChEBI" id="CHEBI:90119"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:48221"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Lipid metabolism; fatty acid beta-oxidation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002956">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6PNW9"/>
    <dbReference type="FunCoup" id="F6PNW9">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000004736"/>
    <dbReference type="Ensembl" id="ENSCINT00000004736.3">
        <property type="protein sequence ID" value="ENSCINP00000004736.3"/>
        <property type="gene ID" value="ENSCING00000002318.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160993"/>
    <dbReference type="HOGENOM" id="CLU_007526_3_0_1"/>
    <dbReference type="InParanoid" id="F6PNW9"/>
    <dbReference type="OMA" id="LHGGHFE"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003995">
        <property type="term" value="F:acyl-CoA dehydrogenase activity"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033539">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA dehydrogenase"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd05154">
        <property type="entry name" value="ACAD10_11_N-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR041726">
        <property type="entry name" value="ACAD10_11_N"/>
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    <dbReference type="InterPro" id="IPR050741">
        <property type="entry name" value="Acyl-CoA_dehydrogenase"/>
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    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002575">
        <property type="entry name" value="Aminoglycoside_PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48083:SF13">
        <property type="entry name" value="ACYL-COA DEHYDROGENASE FAMILY MEMBER 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48083">
        <property type="entry name" value="MEDIUM-CHAIN SPECIFIC ACYL-COA DEHYDROGENASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01636">
        <property type="entry name" value="APH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0274">FAD</keyword>
    <keyword evidence="9" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoglycoside phosphotransferase" evidence="23">
        <location>
            <begin position="19"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase N-terminal" evidence="25">
        <location>
            <begin position="395"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="24">
        <location>
            <begin position="515"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="22">
        <location>
            <begin position="629"/>
            <end position="777"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00040622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00046026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00049140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF01636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000004736.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="789" mass="88755" checksum="C59B04A5D5DAAB88" modified="2025-10-08" version="3"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>F6Q0T6</accession>
    <accession>A0A1W2WE84</accession>
    <name>F6Q0T6_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized LOC100176980</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100176980</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
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                <person name="Richardson P."/>
                <person name="Lucas S."/>
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                <person name="Levine M."/>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">wild type</strain>
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    <reference evidence="7" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="7" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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            <name>heme</name>
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        <text evidence="2 6">Belongs to the cytochrome P450 family.</text>
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</entry>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="5">wild type</strain>
        </source>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
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                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
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                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Putnam N."/>
                <person name="Rash S."/>
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                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
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                <person name="Branno M."/>
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                <person name="Makabe K.W."/>
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                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
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                <person name="Nishizaka S."/>
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                <person name="Oishi K."/>
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                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
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                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">wild type</strain>
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        </citation>
        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
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                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
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                <person name="Terry A."/>
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                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
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                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the dephosphorylation of the nucleoside 5'-monophosphates deoxyadenosine monophosphate (dAMP), deoxycytidine monophosphate (dCMP), deoxyguanosine monophosphate (dGMP) and deoxythymidine monophosphate (dTMP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 2'-deoxyribonucleoside 5'-phosphate + H2O = a 2'-deoxyribonucleoside + phosphate</text>
            <dbReference type="Rhea" id="RHEA:36167"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:18274"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:65317"/>
            <dbReference type="EC" id="3.1.3.89"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the HDDC2 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.89" evidence="7"/>
    <dbReference type="RefSeq" id="XP_002125754.1">
        <property type="nucleotide sequence ID" value="XM_002125718.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6QWU8"/>
    <dbReference type="FunCoup" id="F6QWU8">
        <property type="interactions" value="262"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000013201"/>
    <dbReference type="Ensembl" id="ENSCINT00000013201.3">
        <property type="protein sequence ID" value="ENSCINP00000013201.3"/>
        <property type="gene ID" value="ENSCING00000006407.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100179541"/>
    <dbReference type="KEGG" id="cin:100179541"/>
    <dbReference type="GeneTree" id="ENSGT00390000009937"/>
    <dbReference type="HOGENOM" id="CLU_039453_2_1_1"/>
    <dbReference type="InParanoid" id="F6QWU8"/>
    <dbReference type="OMA" id="TWRLCLM"/>
    <dbReference type="OrthoDB" id="10254258at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002953">
        <property type="term" value="F:5'-deoxynucleotidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="HD domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.10">
        <property type="entry name" value="Hypothetical protein af1432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039356">
        <property type="entry name" value="YfbR/HDDC2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11845">
        <property type="entry name" value="5'-DEOXYNUCLEOTIDASE HDDC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11845:SF13">
        <property type="entry name" value="5'-DEOXYNUCLEOTIDASE HDDC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13023">
        <property type="entry name" value="HD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="47"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001638"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009999"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00032735"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000013201.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="193" mass="22303" checksum="04F823594635101F" modified="2011-07-27" version="1">MSTMTSDTMTSNRMTSDMIKFLTMVGKLKRTKRSGWVMRGVNDPESVSDHMYRMSIMAMLCNNSSTMDKTRCIKLCLIHDMAECIVGDITPYDNVSKEEKHAREKKAMQELSSLLPDEAATEIMELFEEYESQSTEEARYVKDLDRFEMILQARHYEEGEGMCLQEFYDSVDGKIKNQEIISLATTLVESRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>F6R3U4</accession>
    <name>F6R3U4_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">B30.2/SPRY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="FunCoup" id="F6R3U4">
        <property type="interactions" value="521"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000006044"/>
    <dbReference type="Ensembl" id="ENSCINT00000006044.3">
        <property type="protein sequence ID" value="ENSCINP00000006044.3"/>
        <property type="gene ID" value="ENSCING00000002961.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000167826"/>
    <dbReference type="HOGENOM" id="CLU_012140_0_0_1"/>
    <dbReference type="InParanoid" id="F6R3U4"/>
    <dbReference type="OMA" id="FFEMAPR"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000380">
        <property type="term" value="P:alternative mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd12884">
        <property type="entry name" value="SPRY_hnRNP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035778">
        <property type="entry name" value="SPRY_hnRNP_U"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12381:SF56">
        <property type="entry name" value="B30.2_SPRY DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12381">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN U FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13671">
        <property type="entry name" value="AAA_33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B30.2/SPRY" evidence="4">
        <location>
            <begin position="11"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="492"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="492"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="547"/>
            <end position="617"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000006044.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="644" mass="73092" checksum="6E3FC25A39F8AF68" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>F6R6M9</accession>
    <name>F6R6M9_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
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                <person name="Kobayashi K."/>
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                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
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                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
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                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
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                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
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                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
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                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000020005.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="228" mass="26414" checksum="87D1B18B2356D99B" modified="2012-04-18" version="2">MDTDRETLDPNKLANFAMNLKPGTLCNYATSVDHIQQIKPTKDIVENEKISIKPNQHWICPKCKVVFETRGKFDYHKRTHKERVFKRFSCSICAKLFPTKQSLQVHSVVHSQPQDRPFSCKTCSKSFQTKQNLQNHMNIHLDIKPFKCKKCNKSFREQSTLTKHLATHTKEKHHSCSTCNMKFSQASSLQRHVNKFHQNFDNFSGFLPALGAEIVANQLMLLENKLDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F6RHS4</accession>
    <name>F6RHS4_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01000523">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="EAAA01000524">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="EAAA01000525">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6RHS4"/>
    <dbReference type="Ensembl" id="ENSCINT00000025718.2">
        <property type="protein sequence ID" value="ENSCINP00000025472.2"/>
        <property type="gene ID" value="ENSCING00000013992.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01000000220949"/>
    <dbReference type="HOGENOM" id="CLU_180895_2_0_1"/>
    <dbReference type="InParanoid" id="F6RHS4"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003345743" evidence="2">
        <location>
            <begin position="24"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000025472.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="62" mass="7562" checksum="43F6651506DD125F" modified="2012-04-18" version="2">MNKSLFLLLLVIGLLVLTDTTDAWRRRRRGGRRRWWNQKAAKESEDVESIEEPKEDDRWYEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F6RIF9</accession>
    <name>F6RIF9_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Box C/D snoRNA protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Zinc finger HIT domain-containing protein 6</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">wild type</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Required for box C/D snoRNAs accumulation involved in snoRNA processing, snoRNA transport to the nucleolus and ribosome biogenesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Interacts with FBL, SNU13, NOP58, NUFIP1, RUVBL1, RUVBL2 and TAF9. Interacts (via HIT-type zinc finger) with the RUVBL1/RUVBL2 complex in the presence of ADP.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the BCD1 family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01003000">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6RIF9"/>
    <dbReference type="STRING" id="7719.ENSCINP00000013483"/>
    <dbReference type="Ensembl" id="ENSCINT00000013483.3">
        <property type="protein sequence ID" value="ENSCINP00000013483.3"/>
        <property type="gene ID" value="ENSCING00000012888.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000017201"/>
    <dbReference type="HOGENOM" id="CLU_2031785_0_0_1"/>
    <dbReference type="InParanoid" id="F6RIF9"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23023">
        <property type="entry name" value="zf-HIT_BCD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.190:FF:000001">
        <property type="entry name" value="box C/D snoRNA protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051639">
        <property type="entry name" value="BCD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007529">
        <property type="entry name" value="Znf_HIT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13483:SF3">
        <property type="entry name" value="BOX C_D SNORNA PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13483">
        <property type="entry name" value="BOX C_D SNORNA PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04438">
        <property type="entry name" value="zf-HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144232">
        <property type="entry name" value="HIT/MYND zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51083">
        <property type="entry name" value="ZF_HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="HIT-type" evidence="14">
        <location>
            <begin position="38"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00061949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00068630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00077531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000013483.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="122" mass="14090" checksum="ABC1079362CC445F" modified="2012-04-18" version="2">MVNSKDLHTMENFVNSATGNMEQNGQEQSLQKKEVLKCWSCKDKVAKYKCPKCSIRTCSVECVKHHKKLNECDGIRCKTEYVAKENFTETNLLSDYKFLEEVNRCACTNDRNVLPYPKSKNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>F6RIL1</accession>
    <name>F6RIL1_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">wild type</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01000540">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6RIL1"/>
    <dbReference type="STRING" id="7719.ENSCINP00000025455"/>
    <dbReference type="Ensembl" id="ENSCINT00000025701.2">
        <property type="protein sequence ID" value="ENSCINP00000025455.2"/>
        <property type="gene ID" value="ENSCING00000013982.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161150"/>
    <dbReference type="HOGENOM" id="CLU_1506685_0_0_1"/>
    <dbReference type="InParanoid" id="F6RIL1"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050498">
        <property type="entry name" value="Ycf3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44858">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44858:SF1">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE--PEPTIDE N-ACETYLGLUCOSAMINYLTRANSFERASE SPINDLY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13414">
        <property type="entry name" value="TPR_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="49"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="116"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000025455.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="179" mass="20236" checksum="D8A8ACE184521C17" modified="2012-04-18" version="2">MYVLLSKTLMKGKRYKEAITNLKNALELSTLYTYGATTSTANEAPPEAAEIHFLLGQCYTEQLQHTEALQAYNQALKVNPELAEAYYQRGLCRLKLDHSKGIQDLNRALALQPLLFEAYLSRAAYYSTKERYSKAILNCNEALRLRPKSVRALLCRGGLKFRISAYRHALTDLSAAIEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>F6RNF5</accession>
    <name>F6RNF5_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Dynein beta chain, ciliary-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC100180857</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dynein heavy chain family.</text>
    </comment>
    <dbReference type="STRING" id="7719.ENSCINP00000011920"/>
    <dbReference type="Ensembl" id="ENSCINT00000011920.3">
        <property type="protein sequence ID" value="ENSCINP00000011920.3"/>
        <property type="gene ID" value="ENSCING00000005721.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154076"/>
    <dbReference type="InParanoid" id="F6RNF5"/>
    <dbReference type="OMA" id="ICHRDED"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097729">
        <property type="term" value="C:9+2 motile cilium"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008569">
        <property type="term" value="F:minus-end-directed microtubule motor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060294">
        <property type="term" value="P:cilium movement involved in cell motility"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.2620:FF:000004">
        <property type="entry name" value="Dynein axonemal heavy chain 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.20:FF:000003">
        <property type="entry name" value="Dynein axonemal heavy chain 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.30:FF:000003">
        <property type="entry name" value="Dynein axonemal heavy chain 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000219">
        <property type="entry name" value="Dynein axonemal heavy chain 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.180.20:FF:000001">
        <property type="entry name" value="Dynein axonemal heavy chain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.100:FF:000007">
        <property type="entry name" value="Dynein axonemal heavy chain 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000945">
        <property type="entry name" value="Dynein axonemal heavy chain 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002141">
        <property type="entry name" value="Dynein heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.710:FF:000002">
        <property type="entry name" value="dynein heavy chain 17, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1120:FF:000002">
        <property type="entry name" value="Dynein heavy chain 9, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.130:FF:000001">
        <property type="entry name" value="Dynein, axonemal, heavy chain 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.100">
        <property type="entry name" value="Dynein heavy chain, N-terminal domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.180.20">
        <property type="entry name" value="Dynein heavy chain, N-terminal domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035699">
        <property type="entry name" value="AAA_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035706">
        <property type="entry name" value="AAA_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026983">
        <property type="entry name" value="DHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041589">
        <property type="entry name" value="DNAH3_AAA_lid_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042222">
        <property type="entry name" value="Dynein_2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043157">
        <property type="entry name" value="Dynein_AAA1S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041466">
        <property type="entry name" value="Dynein_AAA5_ext"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024743">
        <property type="entry name" value="Dynein_HC_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024317">
        <property type="entry name" value="Dynein_heavy_chain_D4_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013602">
        <property type="entry name" value="Dynein_heavy_linker"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013594">
        <property type="entry name" value="Dynein_heavy_tail"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042228">
        <property type="entry name" value="Dynein_linker_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45703:SF4">
        <property type="entry name" value="DYNEIN AXONEMAL HEAVY CHAIN 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45703">
        <property type="entry name" value="DYNEIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12774">
        <property type="entry name" value="AAA_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12775">
        <property type="entry name" value="AAA_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12780">
        <property type="entry name" value="AAA_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12781">
        <property type="entry name" value="AAA_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17857">
        <property type="entry name" value="AAA_lid_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08385">
        <property type="entry name" value="DHC_N1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08393">
        <property type="entry name" value="DHC_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17852">
        <property type="entry name" value="Dynein_AAA_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12777">
        <property type="entry name" value="MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-0969">Cilium</keyword>
    <keyword evidence="9 14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0243">Dynein</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="11" id="KW-0505">Motor protein</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="15">
        <location>
            <begin position="1851"/>
            <end position="1987"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="15">
        <location>
            <begin position="2129"/>
            <end position="2327"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="15">
        <location>
            <begin position="2456"/>
            <end position="2601"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="3043"/>
            <end position="3087"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="3273"/>
            <end position="3321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000011920.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="3522" mass="402215" checksum="110CD441B87FEAE0" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>F6SJR0</accession>
    <name>F6SJR0_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Peflin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">PEF protein with a long N-terminal hydrophobic domain</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Penta-EF hand domain-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasmic vesicle</location>
            <location evidence="9">COPII-coated vesicle membrane</location>
            <topology evidence="9">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="F6SJR0"/>
    <dbReference type="FunCoup" id="F6SJR0">
        <property type="interactions" value="144"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000027724"/>
    <dbReference type="Ensembl" id="ENSCINT00000027970.2">
        <property type="protein sequence ID" value="ENSCINP00000027724.2"/>
        <property type="gene ID" value="ENSCING00000015777.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155722"/>
    <dbReference type="HOGENOM" id="CLU_051357_1_1_1"/>
    <dbReference type="InParanoid" id="F6SJR0"/>
    <dbReference type="OMA" id="PTICPAY"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012507">
        <property type="term" value="C:ER to Golgi transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048306">
        <property type="term" value="F:calcium-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048208">
        <property type="term" value="P:COPII vesicle coating"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd16183">
        <property type="entry name" value="EFh_PEF_ALG-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000139">
        <property type="entry name" value="Peflin isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051426">
        <property type="entry name" value="Peflin/Sorcin_CaBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46212">
        <property type="entry name" value="PEFLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46212:SF10">
        <property type="entry name" value="PEFLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="13">
        <location>
            <begin position="18"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="13">
        <location>
            <begin position="85"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="13">
        <location>
            <begin position="121"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00041025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00041490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00042606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000027724.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="187" mass="22031" checksum="774C4CA781C833BE" modified="2012-04-18" version="1">PPSYNPQYQQQQPACPPGIDPTVFYWFQAVDQDRSGKINAAELRKALMNNKMKQFNPETCRLMIGMFDKNKDGTIDLREFSALWNYIQQWRQCFDSFDTDKSGNIDCGELHRAFHTFGYRLSMEFCKMIVRVFDKTSANTIDFDDFIQVCVMLHTLTDKFRQNDKNQSGYIQVHYEQFLEMVLDNTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>F6SMS7</accession>
    <name>F6SMS7_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Gelsolin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01001616">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="F6SMS7"/>
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    <dbReference type="STRING" id="7719.ENSCINP00000022359"/>
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        <property type="protein sequence ID" value="ENSCINP00000022359.2"/>
        <property type="gene ID" value="ENSCING00000022118.1"/>
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    <dbReference type="GeneTree" id="ENSGT00940000155591"/>
    <dbReference type="HOGENOM" id="CLU_002568_4_1_1"/>
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    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 3"/>
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        <property type="evidence" value="ECO:0007669"/>
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        <property type="entry name" value="gelsolin_S1_like"/>
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        <property type="entry name" value="gelsolin_S2_like"/>
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        <property type="entry name" value="Gelsolin-like_dom"/>
    </dbReference>
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        <property type="entry name" value="Villin/Gelsolin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11977:SF131">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Actin depolymerizing proteins"/>
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    <keyword evidence="1" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="14"/>
            <end position="96"/>
        </location>
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    <feature type="domain" description="Gelsolin-like" evidence="2">
        <location>
            <begin position="137"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000022359.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="193" mass="22001" checksum="1FB1922BB569AB12" modified="2012-04-18" version="2">MAPKNLKIWRIEDFEMVEQPKSSYGYFFTGDSYLVMNEYKDSDGNTAYDLHMWIGSKSSQDEYGSCAFHAVKLDDEYGGVPVQHRETEGYESSLFMGYFKPAIKYQEGGVASGFNHVEINDYSSVKRLLWVRGRRHVRANVVPLAWSSLNKSDCFVLDMGNTIYTWNGPKCNRFEALQATVVANDVRSNERAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>F6SW71</accession>
    <name>F6SW71_CIOIN</name>
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        <recommendedName>
            <fullName evidence="3 16">Guanylate cyclase</fullName>
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    <organism evidence="20 21">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
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            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">wild type</strain>
        </source>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>GTP = 3',5'-cyclic GMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:13665"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:57746"/>
            <dbReference type="EC" id="4.6.1.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.2" evidence="3 16"/>
    <dbReference type="EMBL" id="EAAA01001123">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000014922"/>
    <dbReference type="Ensembl" id="ENSCINT00000014922.3">
        <property type="protein sequence ID" value="ENSCINP00000014922.3"/>
        <property type="gene ID" value="ENSCING00000007273.3"/>
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    <dbReference type="HOGENOM" id="CLU_001072_1_3_1"/>
    <dbReference type="InParanoid" id="F6SW71"/>
    <dbReference type="OMA" id="XENSSNI"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0004383">
        <property type="term" value="F:guanylate cyclase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0016941">
        <property type="term" value="F:natriuretic peptide receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0017046">
        <property type="term" value="F:peptide hormone binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006182">
        <property type="term" value="P:cGMP biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0007168">
        <property type="term" value="P:receptor guanylyl cyclase signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="PBP1_NPR_GC-like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PK_GC-A_B"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="3"/>
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        <property type="entry name" value="Nucleotide cyclase"/>
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        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="A/G_cyclase"/>
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        <property type="entry name" value="A/G_cyclase_CS"/>
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        <property type="entry name" value="ANF_lig-bd_rcpt"/>
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    <dbReference type="InterPro" id="IPR001170">
        <property type="entry name" value="ANPR/GUC"/>
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    <dbReference type="InterPro" id="IPR050401">
        <property type="entry name" value="Cyclic_nucleotide_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11920:SF494">
        <property type="entry name" value="ATRIAL NATRIURETIC PEPTIDE RECEPTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11920">
        <property type="entry name" value="GUANYLYL CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00255">
        <property type="entry name" value="NATPEPTIDER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00452">
        <property type="entry name" value="GUANYLATE_CYCLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14 16" id="KW-0141">cGMP biosynthesis</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="13 15" id="KW-0456">Lyase</keyword>
    <keyword evidence="10 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="450"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="18">
        <location>
            <begin position="513"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="19">
        <location>
            <begin position="855"/>
            <end position="985"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU003431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000014922.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="988" mass="112894" checksum="D5C3E8494F328A0D" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>F6TB77</accession>
    <name>F6TB77_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">wild type</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001970">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6TB77"/>
    <dbReference type="FunCoup" id="F6TB77">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000006807"/>
    <dbReference type="Ensembl" id="ENSCINT00000006807.3">
        <property type="protein sequence ID" value="ENSCINP00000006807.3"/>
        <property type="gene ID" value="ENSCING00000003317.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157697"/>
    <dbReference type="HOGENOM" id="CLU_001485_16_1_1"/>
    <dbReference type="InParanoid" id="F6TB77"/>
    <dbReference type="OMA" id="DFDLWYQ"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005871">
        <property type="term" value="C:kinesin complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056524">
        <property type="entry name" value="KIF6/9_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968">
        <property type="entry name" value="CENTROMERE PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968:SF67">
        <property type="entry name" value="KINESIN MOTOR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23735">
        <property type="entry name" value="KIF9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0493">Microtubule</keyword>
    <keyword evidence="5 6" id="KW-0505">Motor protein</keyword>
    <keyword evidence="2 5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="8">
        <location>
            <begin position="5"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="700"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="702"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="96"/>
            <end position="103"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000006807.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="790" mass="90622" checksum="147BE52905595DFA" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>F6TGH1</accession>
    <name>F6TGH1_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ATP-binding cassette sub-family F member 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC101243528</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">wild type</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily. ABCF family. EF3 subfamily.</text>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="ENSCINP00000016145.3"/>
        <property type="gene ID" value="ENSCING00000007878.3"/>
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    <dbReference type="GeneTree" id="ENSGT00940000155604"/>
    <dbReference type="HOGENOM" id="CLU_000604_36_6_1"/>
    <dbReference type="InParanoid" id="F6TGH1"/>
    <dbReference type="OMA" id="CTHIADI"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 1"/>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="AAA+_ATPase"/>
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    <dbReference type="InterPro" id="IPR032781">
        <property type="entry name" value="ABC_tran_Xtn"/>
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        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
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        <property type="entry name" value="ABC_transporter-like_CS"/>
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    <dbReference type="InterPro" id="IPR050611">
        <property type="entry name" value="ABCF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
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    <dbReference type="PANTHER" id="PTHR19211:SF117">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY F MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211">
        <property type="entry name" value="ATP-BINDING TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12848">
        <property type="entry name" value="ABC_tran_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="ABC_TRANSPORTER_2"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
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        <location>
            <begin position="116"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="431"/>
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        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="53"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00073919"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
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    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000016145.3"/>
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    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>F6TJ44</accession>
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        <submittedName>
            <fullName evidence="7">RING finger protein 219</fullName>
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    <gene>
        <name evidence="7" type="primary">LOC100185382</name>
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    <organism evidence="7 8">
        <name type="scientific">Ciona intestinalis</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Phlebobranchia</taxon>
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            <taxon>Ciona</taxon>
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    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">wild type</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="F6TJ44"/>
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        <property type="protein sequence ID" value="ENSCINP00000021791.2"/>
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        <property type="component" value="Chromosome 2"/>
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    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0006513">
        <property type="term" value="P:protein monoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006275">
        <property type="term" value="P:regulation of DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="OBI1"/>
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        <property type="entry name" value="OBI1_RING-HC"/>
    </dbReference>
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        <property type="entry name" value="Znf_RING"/>
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        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
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    <dbReference type="PANTHER" id="PTHR14609:SF1">
        <property type="entry name" value="ORC UBIQUITIN LIGASE 1"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR14609">
        <property type="entry name" value="RING FINGER PROTEIN 219"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13923">
        <property type="entry name" value="zf-C3HC4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="19"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="92"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="217"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000021791.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="618" mass="69221" checksum="8EA1464E3E340984" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>F6TPM4</accession>
    <name>F6TPM4_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FAM86 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class I-like SAM-binding methyltransferase superfamily. EEF2KMT family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="F6TPM4"/>
    <dbReference type="FunCoup" id="F6TPM4">
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    </dbReference>
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    <dbReference type="Ensembl" id="ENSCINT00000005575.3">
        <property type="protein sequence ID" value="ENSCINP00000005575.3"/>
        <property type="gene ID" value="ENSCING00000002733.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164788"/>
    <dbReference type="HOGENOM" id="CLU_038942_0_1_1"/>
    <dbReference type="InParanoid" id="F6TPM4"/>
    <dbReference type="OMA" id="CWQASCD"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016279">
        <property type="term" value="F:protein-lysine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Methyltransf_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614">
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614:SF130">
        <property type="entry name" value="PROTEIN-LYSINE N-METHYLTRANSFERASE EEF2KMT"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FAM86 N-terminal" evidence="5">
        <location>
            <begin position="41"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF14904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000005575.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="327" mass="37317" checksum="C32443942E0C585C" modified="2012-04-18" version="2">MDGVCHISTQFLSCVPIKSFVWRKKYMVKDGRCEIHDIDHLQWSEDCQMKVLNKTCFHEIGKRFPPSKKYQQYFLKFLISKVEENNQPCCDQLCELYVATLQDQTCQQVYKSYYVPSANAYATLEESLSFISNGTTGLVTWTAALLLAEWCLSKQDFLRGKKIIELGSGIGFTGIVLLKAVEQLSYTFTDVHPNVLSVLKSNVAINSLENENVAIKQLKWGEQSTILEQPYDIVLAADVVFDPSIIPDLLHTISMLLCRNKDAILVLVSVVRAEKTFQCFLDGLDNIKGLVYEELVNEQTPEVFYFDRNCQWKILVAKSKQCLTSFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>F6U402</accession>
    <name>F6U402_CIOIN</name>
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        <recommendedName>
            <fullName evidence="5">Zinc finger RING-type eukaryotic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
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        <lineage>
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            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">wild type</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01001740">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6U402"/>
    <dbReference type="STRING" id="7719.ENSCINP00000023542"/>
    <dbReference type="Ensembl" id="ENSCINT00000023788.2">
        <property type="protein sequence ID" value="ENSCINP00000023542.2"/>
        <property type="gene ID" value="ENSCING00000012673.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00440000036236"/>
    <dbReference type="HOGENOM" id="CLU_1202199_0_0_1"/>
    <dbReference type="InParanoid" id="F6U402"/>
    <dbReference type="OMA" id="GCARAQI"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Zinc finger RING-type eukaryotic" evidence="5">
        <location>
            <begin position="60"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="117"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="191"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="117"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="143"/>
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        <source>
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        <source>
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    <evidence type="ECO:0000313" key="7">
        <source>
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    <sequence length="231" mass="25393" checksum="07AE07F49E3B32E2" modified="2012-04-18" version="2">MLGALKVWASGQKSSKYSPRNKRKSRPGSRPSSPVPLTCEPQESDVDNTEMVNYGHLLTCPLCDELFTHPVLLPCQHSLCYGCARAQILSDDAMMTSMIEEIADEDSAIEMSAVTSPTYTSSFTSSPPRSVTRKNSDPEFDLSPQSSNSRQSGFPDLENEDPQLMERESGNNNGGNKQSIVLTTKDLLHFKSSARDRTSSTRSLKSGHSTPRSGSFIESDGQRPGFKKFFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
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            <fullName evidence="12">Active breakpoint cluster region-related protein</fullName>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="10" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="10">wild type</strain>
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    <reference evidence="10" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <subcellularLocation>
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            <location evidence="1">Axon</location>
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</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>F6U8B5</accession>
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    <reference evidence="22" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">May contribute to UTP accumulation needed for blast transformation and proliferation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 17">
            <text>cytidine + ATP = CMP + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24674"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17562"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.48"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 17">
            <text>uridine + ATP = UMP + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16825"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16704"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.48"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="17">Pyrimidine metabolism; CTP biosynthesis via salvage pathway; CTP from cytidine: step 1/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 17">Pyrimidine metabolism; UMP biosynthesis via salvage pathway; UMP from uridine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Interacts with RNF19B.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 17">Belongs to the uridine kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.48" evidence="17"/>
    <dbReference type="AlphaFoldDB" id="F6U8B5"/>
    <dbReference type="FunCoup" id="F6U8B5">
        <property type="interactions" value="217"/>
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    <dbReference type="STRING" id="7719.ENSCINP00000007462"/>
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        <property type="protein sequence ID" value="ENSCINP00000007462.3"/>
        <property type="gene ID" value="ENSCING00000003622.3"/>
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    <dbReference type="GeneTree" id="ENSGT01020000230412"/>
    <dbReference type="HOGENOM" id="CLU_021278_0_1_1"/>
    <dbReference type="InParanoid" id="F6U8B5"/>
    <dbReference type="OMA" id="EQAACNE"/>
    <dbReference type="UniPathway" id="UPA00574">
        <property type="reaction ID" value="UER00637"/>
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    <dbReference type="UniPathway" id="UPA00579">
        <property type="reaction ID" value="UER00640"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
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    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:ATP binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:cytidine kinase activity"/>
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        <property type="term" value="F:uridine kinase activity"/>
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        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0044211">
        <property type="term" value="P:CTP salvage"/>
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        <property type="project" value="UniProtKB-UniPathway"/>
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    <dbReference type="GO" id="GO:0044206">
        <property type="term" value="P:UMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
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        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02023">
        <property type="entry name" value="UMPK"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Uridine-cytidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Uridine-cytidine kinase"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="P-loop_NTPase"/>
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        <property type="entry name" value="PRK/URK"/>
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        <property type="entry name" value="PRTase-like"/>
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        <property type="entry name" value="PRTase_dom"/>
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        <property type="entry name" value="Uridine_kinase-like"/>
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        <property type="entry name" value="PRK00129.1"/>
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    </dbReference>
    <dbReference type="NCBIfam" id="NF004018">
        <property type="entry name" value="PRK05480.1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="udk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10285">
        <property type="entry name" value="URIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00485">
        <property type="entry name" value="PRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14681">
        <property type="entry name" value="UPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00988">
        <property type="entry name" value="URIDINKINASE"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 17" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Phosphoribulokinase/uridine kinase" evidence="19">
        <location>
            <begin position="71"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="20">
        <location>
            <begin position="301"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022840"/>
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            <dbReference type="ARBA" id="ARBA00022843"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00047436"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00048909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00056790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00065923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU003825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF00485"/>
        </source>
    </evidence>
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            <dbReference type="Pfam" id="PF14681"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000007462.3"/>
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    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="505" mass="57194" checksum="6010DDAC1CC17131" modified="2012-04-18" version="2">MNGPTKERRLSSRSNSGEAEDKVGATDLGRRKRNASVSEPISVIRTSKRTIYTAGRPPWYNESGEMKEAFIIGLSGGSASGKTTVAEKIIQALDFPWVVLLSMDSFYKVLTHEQHETAERNEYNFDHVDAFDFDLCCETLRKLKQGRSVEIPMYDFTTHSRKKQTKQLYGANVVIFEGIMSFVRPELRELMDMKVFVDADSDERLARRLRRDITLRGRNLQGVLHQYNKFVKPAFEQFIEPTLQYADIVVPRGGSNEVAIDLIAQHVHTQLETVKKSLRKHNLAIAHTGQPLPSTLHVLESSKQVQGIHTILRNRDTNRDDFVFYSKRLLRLLFEFALSLLPHQTHIVETPQSTKYEGRKFSGTGLCGMSILRAGETMEPALFSVTKDIRLGKILIQTNARTEEPELHYIRVPRKISEDHVILMDATVATGAAAMMAIRVLLDHDVKEENILLVSLLMAETGVHSVAYAFPKVQIVTTAVDTHLNEHYHIIPGFGNFGDRYFGTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F6UF65</accession>
    <name>F6UF65_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA repair protein Rev1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01002955">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6UF65"/>
    <dbReference type="Ensembl" id="ENSCINT00000005228.2">
        <property type="protein sequence ID" value="ENSCINP00000005228.2"/>
        <property type="gene ID" value="ENSCING00000002566.2"/>
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    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1280">
        <property type="entry name" value="DNA repair protein Rev1, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031991">
        <property type="entry name" value="Rev1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038401">
        <property type="entry name" value="Rev1_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16727">
        <property type="entry name" value="REV1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA repair protein Rev1 C-terminal" evidence="1">
        <location>
            <begin position="70"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000005228.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="171" mass="19380" checksum="E77707DF761D409A" modified="2011-07-27" version="1">MLPLKAVTSPNKLITDMFSKIPKKEVGKVDPVAKRSLEDDVTICEGKNDNTKTERKISKPSLGGATTTAEVRDVIRKWTRAYSQEFPLDSDIEDIINYLCTLVNHVRNLESTELILRTIRRYIGFVIEQNENVEMQTAWLVAYSQILCAVNKKVENLYGGAQFQLPSLHLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>F6UPT3</accession>
    <name>F6UPT3_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 13 14">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism; bile acid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="F6UPT3"/>
    <dbReference type="FunCoup" id="F6UPT3">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000028684"/>
    <dbReference type="Ensembl" id="ENSCINT00000028930.2">
        <property type="protein sequence ID" value="ENSCINP00000028684.2"/>
        <property type="gene ID" value="ENSCING00000016619.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153141"/>
    <dbReference type="HOGENOM" id="CLU_018012_1_3_1"/>
    <dbReference type="InParanoid" id="F6UPT3"/>
    <dbReference type="OMA" id="FVFRSVM"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008395">
        <property type="term" value="F:steroid hydroxylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006699">
        <property type="term" value="P:bile acid biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042632">
        <property type="term" value="P:cholesterol homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000304">
        <property type="entry name" value="25-hydroxycholesterol 7-alpha-hydroxylase-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050529">
        <property type="entry name" value="CYP450_sterol_14alpha_dmase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024204">
        <property type="entry name" value="Cyt_P450_CYP7A1-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002403">
        <property type="entry name" value="Cyt_P450_E_grp-IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24304:SF4">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24304">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000047">
        <property type="entry name" value="Cytochrome_CYPVIIA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00465">
        <property type="entry name" value="EP450IV"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 13 14" id="KW-0349">Heme</keyword>
    <keyword evidence="9 13 14" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0753">Steroid metabolism</keyword>
    <feature type="binding site" evidence="15">
        <location>
            <position position="275"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="14">
        <location>
            <position position="432"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR000047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000047-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR000047-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000028684.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="488" mass="56438" checksum="8539450247F53572" modified="2012-04-18" version="2">MLSVILAFFVLILATYFLWNSRKRQQRDGEPNIVPYSIPYLGRAINFYKDPVKFLKECEEKYGSVFTLFMGGQYFTFVTDPFVQHIIVKEKKVLRFRNCLPPVLKHAFDYYPTAVDDEMMKLTHHYFQGKPLGLLVKQMNRNIQKWILDGNQGDAGDKWETVGLLSLVQRVMGSASFVSLFGENPKASDTLDVQRQLLNFLTVFPSLLRGLPLSLITRSREYRQDLWKSLTKSKVTKRKEIFPIIDEIVTLHEQNGENKLLPKRLLPVLVASQVNTNPSSFWILYYLLKNSEALAAVKDEMEKVLQERSKNPGNNFVCFTKEEMETMTTLDTESAINESLRLSISGGMLRKAATEYRLEIPDYGQTYTIRENDMVLVWTQVNQMDPEIFEEPEVFKYDRFLNEDGSLKTNFYKDGKLVKYAFQPFGDGSSKCPGRYWAVLELKQLVVNMLLYYDIKLIDSQEIKMDKGRVGLGALNPTKDALMSIRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>F6V5E8</accession>
    <name>F6V5E8_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="F6V5E8"/>
    <dbReference type="STRING" id="7719.ENSCINP00000012370"/>
    <dbReference type="Ensembl" id="ENSCINT00000012370.3">
        <property type="protein sequence ID" value="ENSCINP00000012370.3"/>
        <property type="gene ID" value="ENSCING00000005990.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000007020"/>
    <dbReference type="HOGENOM" id="CLU_677133_0_0_1"/>
    <dbReference type="InParanoid" id="F6V5E8"/>
    <dbReference type="OMA" id="NTRILHQ"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026652">
        <property type="entry name" value="CEP128"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46657">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 128 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46657:SF1">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 128 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="101"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="183"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="327"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="9"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="141"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="197"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000012370.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="477" mass="55326" checksum="8AA261CB1C3BADD5" modified="2012-04-18" version="2">MPSRKLHYDSSSSDSSYHTSYASRRRLPSPPPSGILRNTNRHVTIIDPAMMTRRVDRITDNLEDANRNLQSVDSKISSFKDVHDDSMSALTKLQEDLEGSIHRLRNTREKRSFTRDTGSMRRSELNRRSASTTRFGSRDASLNREYDSDTTARRRPSSSLGVGSRKFGDGDFSSTARDRLLDLSASQNRMEQDLNDEISRRLKSEKDTQRSIRDLADSRKNESASERVERRLKQIERGLEVDRNNATHAASQVAQDLLRRSQSKTSEDDSASRSRLLQMESGKRLVENELEATRRRLEQSEGGREALISQIGELRALINRSDKERGELQKQIQKQEAERKAQTEREAQDRLKLAASDRMERERTRQEGEIDELRAALKRSAGVVQLDDLRRDLEKSERQRQHLSDHIEVLTKDLEKKEQTNTRILHQLNELQRDFDESERDRDRVCHQLEDALLKVKEQTREAEKYTKNVQRMEEGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F6V7Z8</accession>
    <name>F6V7Z8_CIOIN</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Phlebobranchia</taxon>
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            <taxon>Ciona</taxon>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">wild type</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01002217">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6V7Z8"/>
    <dbReference type="Ensembl" id="ENSCINT00000023067.2">
        <property type="protein sequence ID" value="ENSCINP00000022821.2"/>
        <property type="gene ID" value="ENSCING00000012124.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000013511"/>
    <dbReference type="HOGENOM" id="CLU_867503_0_0_1"/>
    <dbReference type="InParanoid" id="F6V7Z8"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000022821.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="321" mass="36936" checksum="95CFEBC5ED55325A" modified="2012-04-18" version="2">MLKKFAVIIFTLACFTTFLTIQQTSRYHLSMKMTAIDARIRRQRAIHTDTKLTHKSGMAMEKSSSNSNNARQIDKLVQKYYNNLFATDEYKAVGPKKRKPDMNNLLIPFLFYNMGPNNLFRIFKESIPIIHMMNRTLVVPPFHHHPRMKRLNEEDQSDKTVEVDVDIFDQTYRSFVETDAKKTVDMKTLQYIIPAIPIKRFHSTCGNKINTLITCGIVSGKRASGLTHFKQVTNLTVGRVFKVEHLKDVSSDKEWKMNVMTAVQNTKGQKCVGLVLGSKCLGSRGAWFRHWRSFAPFVRHPPKIQVLANLFTENVLKGEPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>F6VBG0</accession>
    <name>F6VBG0_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidase S1 domain-containing protein</fullName>
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    <organism evidence="5 6">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
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    <reference evidence="6" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">wild type</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="STRING" id="7719.ENSCINP00000022549"/>
    <dbReference type="Ensembl" id="ENSCINT00000022795.2">
        <property type="protein sequence ID" value="ENSCINP00000022549.2"/>
        <property type="gene ID" value="ENSCING00000011927.2"/>
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    <dbReference type="InParanoid" id="F6VBG0"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Peptidase_S1_PA"/>
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    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252">
        <property type="entry name" value="ACROSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252:SF27">
        <property type="entry name" value="TRANSMEMBRANE PROTEASE SERINE 3-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
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    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
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        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003344509" description="Peptidase S1 domain-containing protein" evidence="3">
        <location>
            <begin position="21"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="4">
        <location>
            <begin position="105"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000022549.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>F6VSF8</accession>
    <name>F6VSF8_CIOIN</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
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                <person name="Hino K."/>
                <person name="Imai K.S."/>
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                <person name="Kano S."/>
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                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
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                <person name="Miura S."/>
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                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
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                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="4" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4">wild type</strain>
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    <reference evidence="4" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <keyword evidence="1 4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014090218" description="ZP domain-containing protein" evidence="4">
        <location>
            <begin position="21"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="369"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="ZP" evidence="5">
        <location>
            <begin position="50"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000028313.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="432" mass="48769" checksum="1502A38C32B2DC35" modified="2012-04-18" version="2">MMTFVRLACVILTCVASVYGQMVIVGGKDMNVTTETMKREFLSPGTVKVDCTPTVMCVSVSKQWLADTWQVHDPLDLSLNLRCDQSAGHNLTHVTLCTANQQLSTCGTEFEVNDTYVIYSNNITVRREMNVTSQVRRSFPWRCVYPVRILVGVKYIPIMSQVTHFLGKKTAKGEFRAGMFMFTNSRYTTIYQTPPSLNMDDKIRVRVKLIQGPSFTKMQLYRCWGTPSPLSSQPQQEYELINNFCPSPMSQEANVTIVSNGDLAHATWESSVFRFVNSTKLYLHCNVKLCFSGAPCVKTCTGNTLNRKRRDLGLDNAVVSLGPINPSREVPIVMQQPPVYDVIMDDVTQQTMIVNRQRENFLLGMPRTAVYITIGILVFISIVLAIVATICIVRRKRNSKRVVITSRKKHCDITRARSIAMSSNFEIPITVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F6W8Q0</accession>
    <name>F6W8Q0_CIOIN</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01002558">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6W8Q0"/>
    <dbReference type="Ensembl" id="ENSCINT00000026784.2">
        <property type="protein sequence ID" value="ENSCINP00000026538.2"/>
        <property type="gene ID" value="ENSCING00000014761.2"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 7"/>
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    <proteinExistence type="predicted"/>
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        <location>
            <begin position="1"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="37"/>
        </location>
    </feature>
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        <location>
            <begin position="55"/>
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        </location>
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            <begin position="140"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000026538.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="221" mass="26482" checksum="95121EBC47D33302" modified="2012-04-18" version="2">MADVDYYQSGNPRYDSRGYDDRYRGNQRDSWNERGYHDNQPQQQQGNHGYEQRGYQEQYTTPPTSGSNSNNERYERPTNNQRDTDYQGGYRREDERYDSRNEYQPQYEQRTNQQPNNERWNSINEQPNYNEYNQTNYNRMYDDQRPHNDPLPRPHDPLPNHTRPYDDPQPHNGPQSNYTRPHAPQPHPTHRDEGYRENAIQPKLLAQTAVSIFGVLQLCLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>F6WN54</accession>
    <name>F6WN54_CIOIN</name>
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        <recommendedName>
            <fullName evidence="5">Rab3 GTPase-activating protein catalytic subunit</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Tunicata</taxon>
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    <reference evidence="14" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">wild type</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">cis-Golgi network</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the Rab3-GAP catalytic subunit family.</text>
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    <dbReference type="EMBL" id="EAAA01002232">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6WN54"/>
    <dbReference type="FunCoup" id="F6WN54">
        <property type="interactions" value="731"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000018722"/>
    <dbReference type="Ensembl" id="ENSCINT00000018722.3">
        <property type="protein sequence ID" value="ENSCINP00000018722.3"/>
        <property type="gene ID" value="ENSCING00000009210.4"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000006705"/>
    <dbReference type="InParanoid" id="F6WN54"/>
    <dbReference type="OMA" id="KYAKHRR"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000786">
        <property type="term" value="P:positive regulation of autophagosome assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045700">
        <property type="entry name" value="Rab3GAP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045698">
        <property type="entry name" value="Rab3GAP1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026147">
        <property type="entry name" value="Rab3GAP1_conserved"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21422">
        <property type="entry name" value="RAB3 GTPASE-ACTIVATING PROTEIN CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21422:SF9">
        <property type="entry name" value="RAB3 GTPASE-ACTIVATING PROTEIN CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19533">
        <property type="entry name" value="Rab3-GAP_cat_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13890">
        <property type="entry name" value="Rab3-GTPase_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="623"/>
            <end position="763"/>
        </location>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Ueno K."/>
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                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
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                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="F6X0A3"/>
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        <property type="protein sequence ID" value="ENSCINP00000029464.2"/>
        <property type="gene ID" value="ENSCING00000017384.2"/>
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    <dbReference type="GeneTree" id="ENSGT00660000096720"/>
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        <property type="component" value="Chromosome 1"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003345735" description="Secreted protein" evidence="1">
        <location>
            <begin position="34"/>
            <end position="82"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000029464.2"/>
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        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="82" mass="9393" checksum="6E7320CB68B32FBA" modified="2012-04-18" version="2">MLHIYCFVLQMNKSALLTLLLVGLLVHTETASAHWNTKSLKDDLKLRDEGYYNAVPKDEYYDGYYGAVPKDDHYDGYYGAVP</sequence>
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<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>F6XUS6</accession>
    <name>F6XUS6_CIOIN</name>
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        <recommendedName>
            <fullName evidence="13">Acyl-CoA dehydrogenase family member 10</fullName>
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    <organism evidence="11 12">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="11">wild type</strain>
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    <reference evidence="11" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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    <reference evidence="11" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acyl-CoA dehydrogenase family.</text>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="STRING" id="7719.ENSCINP00000008481"/>
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        <property type="gene ID" value="ENSCING00000004111.4"/>
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    <dbReference type="HOGENOM" id="CLU_007526_2_0_1"/>
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        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003995">
        <property type="term" value="F:acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0033539">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA dehydrogenase"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd05154">
        <property type="entry name" value="ACAD10_11_N-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02603">
        <property type="entry name" value="HAD_sEH-N_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Acyl-CoA dehydrogenase fadE12"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
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        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
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    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="entry name" value="Putative phosphatase, domain 2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="AcylCo_DH-like_C"/>
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        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
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        <property type="entry name" value="Aminoglycoside_PTrfase"/>
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        <property type="entry name" value="HAD-like_sf"/>
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        <property type="entry name" value="HAD-SF_hydro_IA"/>
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        <property type="entry name" value="HAD-SF_ppase_IA/epoxid_hydro_N"/>
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        <property type="entry name" value="HAD_sf"/>
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        <property type="entry name" value="Kinase-like_dom_sf"/>
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        <property type="entry name" value="PGP-like_dom2"/>
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        <property type="entry name" value="HAD-1A3-hyp"/>
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        <property type="entry name" value="HAD-SF-IA-v3"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR47829">
        <property type="entry name" value="HYDROLASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G12880)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF01636">
        <property type="entry name" value="APH"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HADHALOGNASE"/>
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    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
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        <property type="entry name" value="Protein kinase-like (PK-like)"/>
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    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
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            <begin position="251"/>
            <end position="466"/>
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        <location>
            <begin position="625"/>
            <end position="744"/>
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    </feature>
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        <location>
            <begin position="748"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="7">
        <location>
            <begin position="861"/>
            <end position="1010"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000008481.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1013" mass="113405" checksum="49209E6A7EA7D405" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>F6XWG9</accession>
    <name>F6XWG9_CIOIN</name>
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        <recommendedName>
            <fullName evidence="9">Ubiquitin-conjugating enzyme E2 G2</fullName>
            <ecNumber evidence="2">2.3.2.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">E2 ubiquitin-conjugating enzyme G2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Ubiquitin carrier protein G2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Ubiquitin-protein ligase G2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">wild type</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Involved in endoplasmic reticulum-associated degradation (ERAD). Required for sterol-induced ubiquitination of 3-hydroxy-3-methylglutaryl coenzyme A reductase and its subsequent proteasomal degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.</text>
            <dbReference type="EC" id="2.3.2.23"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Interacts with AUP1 (via C-terminus); the interaction recruits UBE2G2 to lipid droplets. Interacts with ubiquitin ligases AMFR/gp78 and RNF139/TRC8; recruitment to lipid droplets by AUP1 facilitates interaction of UBE2G2 with AMFR and RNF139, leading to sterol-induced ubiquitination of 3-hydroxy-3-methylglutaryl coenzyme A reductase and its subsequent proteasomal degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="2"/>
    <dbReference type="EMBL" id="EAAA01002715">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6XWG9"/>
    <dbReference type="FunCoup" id="F6XWG9">
        <property type="interactions" value="341"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000015354"/>
    <dbReference type="Ensembl" id="ENSCINT00000015354.3">
        <property type="protein sequence ID" value="ENSCINP00000015354.3"/>
        <property type="gene ID" value="ENSCING00000007488.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158193"/>
    <dbReference type="HOGENOM" id="CLU_030988_10_1_1"/>
    <dbReference type="InParanoid" id="F6XWG9"/>
    <dbReference type="OMA" id="APDGMFT"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd23796">
        <property type="entry name" value="UBCc_UBE2G2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000008">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 G2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 14" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="15">
        <location>
            <begin position="4"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="13">
        <location>
            <position position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00055690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00063420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00073285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00076340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00079258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00079959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000015354.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="165" mass="18568" checksum="E519B5F74E031669" modified="2012-04-18" version="2">MVGSALKRLMAEYKQLSLNPPEGIVAGPINEDNFFEWEALILGPEDTPFEGGVFRTTLNFPTDYPMSPPKMKFTSDMFHPNIYPDGRVCISILHSPGDDPMGYETSAERWSPVQSIEKILLSVMSMLAEPNPESGANVDASKMWRDDQEQFVKRAEKLVRKSLDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>F6Y0R0</accession>
    <name>F6Y0R0_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized LOC100180999</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC100180999</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
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            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="ENSCING00000006742.3"/>
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        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="146"/>
            <end position="183"/>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="225"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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            <end position="38"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="1">
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            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>F6YF45</accession>
    <accession>A0A1W2WBM2</accession>
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        <recommendedName>
            <fullName evidence="11 12">U6 snRNA-associated Sm-like protein LSm4</fullName>
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        <name evidence="15" type="primary">LOC100184512</name>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Phlebobranchia</taxon>
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            <taxon>Ciona</taxon>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">wild type</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="12">Binds specifically to the 3'-terminal U-tract of U6 snRNA.</text>
    </comment>
    <comment type="function">
        <text evidence="9">Plays a role in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex that is involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex). The heptameric LSM2-8 complex binds specifically to the 3'-terminal U-tract of U6 snRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the precatalytic spliceosome (spliceosome B complex). Component of the U4/U6-U5 tri-snRNP complex, a building block of the precatalytic spliceosome (spliceosome B complex). The U4/U6-U5 tri-snRNP complex is composed of the U4, U6 and U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200, TXNL4A, SNRNP40, SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, SNRPG, DDX23, CD2BP2, PPIH, SNU13, EFTUD2, SART1 and USP39, plus LSM2, LSM3, LSM4, LSM5, LSM6, LSM7 and LSM8. LSM2, LSM3, LSM4, LSM5, LSM6, LSM7 and LSM8 form a heptameric, ring-shaped subcomplex (the LSM2-8 complex) that is part of the U4/U6-U5 tri-snRNP complex and the precatalytic spliceosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">LSm subunits form a heteromer with a doughnut shape.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the snRNP Sm proteins family.</text>
    </comment>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_002128105.5"/>
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        <property type="interactions" value="311"/>
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    <dbReference type="Ensembl" id="ENSCINT00000024527.2">
        <property type="protein sequence ID" value="ENSCINP00000024281.2"/>
        <property type="gene ID" value="ENSCING00000007519.3"/>
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    <dbReference type="KEGG" id="cin:100184512"/>
    <dbReference type="GeneTree" id="ENSGT00610000086173"/>
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    <dbReference type="OMA" id="VACDAWM"/>
    <dbReference type="OrthoDB" id="747253at2759"/>
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        <property type="component" value="Chromosome 8"/>
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        <property type="term" value="C:Lsm2-8 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="C:spliceosomal complex"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="C:spliceosomal tri-snRNP complex"/>
        <property type="evidence" value="ECO:0000318"/>
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        <property type="term" value="C:U6 snRNP"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017070">
        <property type="term" value="F:U6 snRNA binding"/>
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        <property type="project" value="GO_Central"/>
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        <property type="term" value="P:nuclear-transcribed mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
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        <property type="term" value="P:P-body assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="P:spliceosomal snRNP assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="LSm4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="U6 snRNA-associated Sm-like protein LSm4"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034101">
        <property type="entry name" value="Lsm4"/>
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        <property type="entry name" value="LSm4/Sm_D1/D3"/>
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        <property type="entry name" value="LSM_dom_sf"/>
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        <property type="entry name" value="Sm"/>
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    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23338">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="6 12" id="KW-0508">mRNA splicing</keyword>
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    <keyword evidence="8 12" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 12" id="KW-0747">Spliceosome</keyword>
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        <location>
            <begin position="2"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="80"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="90"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="116"/>
            <end position="127"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006850"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
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        <source>
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        <source>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00067757"/>
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        <source>
            <dbReference type="RuleBase" id="RU365049"/>
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    </evidence>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="Ensembl" id="ENSCINP00000024281.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="127" mass="14035" checksum="E66E29E53B2CD00B" modified="2012-04-18" version="2">MLPLSLLKTAVNHPMLVELKNGETYNGHLVSCDNWMNINLREVICTSRDGDHFWRMPECYIRGSTIKYLRIPDEVIDMVKEDSMSKGRGRVGPGRGRGKAVFGGRGRGGSGQGRGGAKKSSRGKSRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>F6YL52</accession>
    <name>F6YL52_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Receptor tyrosine-protein kinase erbB-2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100179961</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
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                <person name="Matassi G."/>
                <person name="Medina M."/>
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                <person name="Nishizaka S."/>
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                <person name="Oishi K."/>
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                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
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                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
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                <consortium name="Ensembl"/>
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</entry>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
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                <person name="Meinertzhagen I.A."/>
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                <person name="Putnam N."/>
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                <person name="Terry A."/>
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                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
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                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="6" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="6">wild type</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May play some role in mitochondrial processes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the OPA3 family.</text>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_004225795.4"/>
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    </dbReference>
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    <dbReference type="Ensembl" id="ENSCINT00000023412.2">
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        <property type="gene ID" value="ENSCING00000012402.2"/>
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    <dbReference type="GeneID" id="100185809"/>
    <dbReference type="GeneTree" id="ENSGT00390000009795"/>
    <dbReference type="HOGENOM" id="CLU_1474675_0_0_1"/>
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    <dbReference type="PANTHER" id="PTHR12499:SF0">
        <property type="entry name" value="OPTIC ATROPHY 3 PROTEIN"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="OPTIC ATROPHY 3 PROTEIN OPA3"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007584"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000023166.2"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    </evidence>
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    <accession>F6YTM1</accession>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
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            <taxon>Ciona</taxon>
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    <reference evidence="10" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">wild type</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="F6YTM1"/>
    <dbReference type="STRING" id="7719.ENSCINP00000007306"/>
    <dbReference type="Ensembl" id="ENSCINT00000007306.3">
        <property type="protein sequence ID" value="ENSCINP00000007306.3"/>
        <property type="gene ID" value="ENSCING00000003553.3"/>
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    <dbReference type="GeneTree" id="ENSGT00530000068687"/>
    <dbReference type="HOGENOM" id="CLU_003230_0_0_1"/>
    <dbReference type="InParanoid" id="F6YTM1"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 7"/>
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    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016758">
        <property type="term" value="F:hexosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030244">
        <property type="term" value="P:cellulose biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06421">
        <property type="entry name" value="CESA_CelA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036434">
        <property type="entry name" value="Beta_cellobiohydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050321">
        <property type="entry name" value="Glycosyltr_2/OpgH_subfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43867">
        <property type="entry name" value="CELLULOSE SYNTHASE CATALYTIC SUBUNIT A [UDP-FORMING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43867:SF2">
        <property type="entry name" value="CELLULOSE SYNTHASE CATALYTIC SUBUNIT A [UDP-FORMING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13641">
        <property type="entry name" value="Glyco_tranf_2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Glycosyl hydrolases family 6, cellulases"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
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    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
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    <feature type="transmembrane region" description="Helical" evidence="8">
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    <feature type="transmembrane region" description="Helical" evidence="8">
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    <feature type="transmembrane region" description="Helical" evidence="8">
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    <feature type="transmembrane region" description="Helical" evidence="8">
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    <feature type="transmembrane region" description="Helical" evidence="8">
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            <begin position="815"/>
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    <feature type="region of interest" description="Disordered" evidence="7">
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
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        <source>
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    </evidence>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
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                <person name="Hayashi H."/>
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                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
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                <person name="Morishita T."/>
                <person name="Miura S."/>
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                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="12">wild type</strain>
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            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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            <topology evidence="1">Multi-pass membrane protein</topology>
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        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
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                <person name="Branno M."/>
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                <person name="Makabe K.W."/>
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                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
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                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
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                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
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                <person name="Wiley G.B."/>
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                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="2" key="3">
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                <consortium name="Ensembl"/>
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            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="273"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000022476.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="368" mass="43349" checksum="3835D3F49A433043" modified="2012-04-18" version="2">MFVLFKQCGNISTNILPYLLFSRLKKGDMARRNTKHGWTDTQVFTYPLPPNKTQLFTKYRYVERPHTAAVTYLEKMLTKTSLTTQKVTHQDIENFGNNVYKNKVKMYEQEKHEAVERAVEETKREAKLQAEKVLAAALDKANVEKETALEKQKNYYEEIKKRIIFGRDCLEKERTKQLVVKMKKEKELALKEQWEKAERIKKETVVEALKEQKRVLRAESALERETAIARALSVARGKFDQKIEEEVEKVREECWLVGKEQLNRSKIIHKHEIERLHERIAVLTQESKKVEKKRDLAQRDFINIESDYSHFLDFTTPVSCHSDYLITPRKLKHGMAESRQEQQTQTNLIALDQKTQSVQTNVQILARR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>F6ZKJ6</accession>
    <name>F6ZKJ6_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Fanconi-associated nuclease</fullName>
            <ecNumber evidence="1">3.1.4.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Nuclease required for the repair of DNA interstrand cross-links (ICL). Acts as a 5'-3' exonuclease that anchors at a cut end of DNA and cleaves DNA successively at every third nucleotide, allowing to excise an ICL from one strand through flanking incisions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolytically removes 5'-nucleotides successively from the 3'-hydroxy termini of 3'-hydroxy-terminated oligonucleotides.</text>
            <dbReference type="EC" id="3.1.4.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAN1 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.1" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="F6ZKJ6"/>
    <dbReference type="STRING" id="7719.ENSCINP00000027539"/>
    <dbReference type="Ensembl" id="ENSCINT00000027785.1">
        <property type="protein sequence ID" value="ENSCINP00000027539.1"/>
        <property type="gene ID" value="ENSCING00000015602.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000018637"/>
    <dbReference type="HOGENOM" id="CLU_1296954_0_0_1"/>
    <dbReference type="InParanoid" id="F6ZKJ6"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004528">
        <property type="term" value="F:phosphodiesterase I activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036297">
        <property type="term" value="P:interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033315">
        <property type="entry name" value="Fan1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049138">
        <property type="entry name" value="Fan1_SAP_met"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15749">
        <property type="entry name" value="FANCONI-ASSOCIATED NUCLEASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15749:SF4">
        <property type="entry name" value="FANCONI-ASSOCIATED NUCLEASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21169">
        <property type="entry name" value="Fan1_SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0227">DNA damage</keyword>
    <keyword evidence="1" id="KW-0234">DNA repair</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0464">Manganese</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fanconi-associated nuclease 1 SAP subdomain metazoa" evidence="2">
        <location>
            <begin position="18"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU365033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF21169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000027539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="213" mass="23871" checksum="F4510969E463C88F" modified="2011-07-27" version="1">MLASHDFLQSSMNEILSLQEILGLLSAPDVKVLAKSFRLSVQGPKPKICDAIESMCKKQKSVFTTSSGQLTSTVTKQALSLLGTCYRINRNVKKVFNRLLLVFDVTKGTEDDTDFTISIHSSNNLQHRKHQQLSAVLMEGMGRVAYPRYEVNRPTPIFGNRDELLEYEIAYQFLIDAISASDVSQWKVADDVCTQATERTPPTPCNKQLPVFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>F6ZP02</accession>
    <name>F6ZP02_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pre-mRNA-splicing factor 38</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">wild type</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Required for pre-mRNA splicing.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the PRP38 family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002182">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6ZP02"/>
    <dbReference type="FunCoup" id="F6ZP02">
        <property type="interactions" value="715"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000006629"/>
    <dbReference type="Ensembl" id="ENSCINT00000006629.3">
        <property type="protein sequence ID" value="ENSCINP00000006629.3"/>
        <property type="gene ID" value="ENSCING00000003237.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165196"/>
    <dbReference type="HOGENOM" id="CLU_039466_1_0_1"/>
    <dbReference type="InParanoid" id="F6ZP02"/>
    <dbReference type="OMA" id="GEHFKYL"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005037">
        <property type="entry name" value="PRP38"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23142">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR 38A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03371">
        <property type="entry name" value="PRP38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5 7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0747">Spliceosome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="185"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="185"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="212"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="261"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="287"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000006629.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="323" mass="38561" checksum="364C96C8EDA45658" modified="2012-04-18" version="2">MANRTVKDAHTVKGTNPQYLIEKIIRSRIYDSRYWKEQCFALTAELLVDKAMDLKYIGGVYSGNIKPCPFLCLILKMLQIQPDKDIIVEFIRNEDFKYVRCLGAFYMRITGTSLDCYKYLEPLLNDFRKIKFQKREGNFVITHMDEFIDELLREERSCDVILPRIQKRQILEELEQIDPRISALDDDLDDLGESSDDDDEGPPLEIRERAPIHRRRSPSPRDRHRRSPSPKHRRSRSRSPRRRRRRKHCISRTPPRRHRSRERERDRDRERQRSPHRSHKDKDHESRSKKKSSKHSKKAKSEVDIEIQEANELRAKLGLKPLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>F6ZQI8</accession>
    <name>F6ZQI8_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glycoside hydrolase family 31 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">wild type</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the glycosyl hydrolase 31 family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01000017">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="EAAA01000018">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6ZQI8"/>
    <dbReference type="STRING" id="7719.ENSCINP00000009390"/>
    <dbReference type="Ensembl" id="ENSCINT00000009390.3">
        <property type="protein sequence ID" value="ENSCINP00000009390.3"/>
        <property type="gene ID" value="ENSCING00000004544.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000170567"/>
    <dbReference type="HOGENOM" id="CLU_017110_2_0_1"/>
    <dbReference type="InParanoid" id="F6ZQI8"/>
    <dbReference type="OMA" id="WAWDESQ"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048395">
        <property type="entry name" value="Glyco_hydro_31_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000322">
        <property type="entry name" value="Glyco_hydro_31_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052990">
        <property type="entry name" value="Sulfoquinovosidase_GH31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46959">
        <property type="entry name" value="SULFOQUINOVOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46959:SF2">
        <property type="entry name" value="SULFOQUINOVOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01055">
        <property type="entry name" value="Glyco_hydro_31_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21365">
        <property type="entry name" value="Glyco_hydro_31_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycoside hydrolase family 31 TIM barrel" evidence="3">
        <location>
            <begin position="2"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 31 C-terminal" evidence="4">
        <location>
            <begin position="242"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU361185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF21365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000009390.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="384" mass="44051" checksum="ABB2E0F299D8EBF0" modified="2012-04-18" version="2">MFQEADNNGYFIKSKSTGKTQLVDFGEMFCGTIDLTNPNAFEWYKQVVKDMLSFGFAGWMADFGGEYLPVDDTMYFDGTHAVEMHNLYSEMWARLNKEAVEEEGKMNDTFVFMRSGFGRSPGHTMTSWAGDQNVDFSYADGLASVIPAALSLGMSGMGIHHSDIGGYVSLICMVRTEELVLRWAEMNVFTPIMRTHEGNRPYRNWQIYTMDTTTESFARLTSLYHRLSPYHSFVAEETASKGIPAQRPLFLHYSDDPTTYDIQYQYMYGPDILVAPVLFEDKHDWSVYLPPDQWVYLWNGTEVVVPRSRDGTGMSVTVNAEMGNPPVFYRKGSPWSKLFQTFAKDEVKPGRTPEGLSLKLLLKEPNIFLFLLLRKMFWYVFFNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>F6ZSW4</accession>
    <name>F6ZSW4_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">wild type</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01000741">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6ZSW4"/>
    <dbReference type="Ensembl" id="ENSCINT00000019856.3">
        <property type="protein sequence ID" value="ENSCINP00000019856.3"/>
        <property type="gene ID" value="ENSCING00000009774.3"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1829657_0_0_1"/>
    <dbReference type="InParanoid" id="F6ZSW4"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000019856.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="141" mass="16218" checksum="E898640B58C5627F" modified="2012-04-18" version="2">MPNNQINSKENTTAPSVWKGAEVQLNRNKTTTAISSSNKPFTICQDVDVPSQEQATPLASRKLSKSVEVILTERKWKKHEESDFHRAIREQHGDADHNVVRMYPVEKVYSAVGEFQPEEILAACWLKKQREEEEQKRLQSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>F6ZUD0</accession>
    <name>F6ZUD0_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Mitotic checkpoint protein BUB3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC100186469</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="17" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="17">wild type</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Centromere</location>
            <location evidence="2">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the WD repeat BUB3 family.</text>
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        <property type="entry name" value="WD_REPEATS_REGION"/>
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    <keyword evidence="5" id="KW-0132">Cell division</keyword>
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    <keyword evidence="9" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="11" id="KW-0469">Meiosis</keyword>
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    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
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        <source>
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            <dbReference type="ARBA" id="ARBA00022838"/>
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            <dbReference type="ARBA" id="ARBA00023242"/>
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<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
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    </protein>
    <gene>
        <name evidence="9" type="primary">ap4</name>
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    <organism evidence="9 10">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
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    <reference evidence="10" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">wild type</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <comment type="subcellular location">
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            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="ENSCING00000014406.2"/>
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        <property type="component" value="Chromosome 14"/>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="evidence" value="ECO:0000318"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd11419">
        <property type="entry name" value="bHLHzip_TFAP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000036">
        <property type="entry name" value="Transcription factor AP-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052207">
        <property type="entry name" value="Max-like/E-box_TFs"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15741">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX ZIP TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15741:SF27">
        <property type="entry name" value="TRANSCRIPTION FACTOR AP-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="8">
        <location>
            <begin position="97"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="561"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="198"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000026074.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="684" mass="75693" checksum="7D87EC13F10D4472" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>F7A5V7</accession>
    <name>F7A5V7_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pleiotropic regulator 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">wild type</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Involved in pre-mRNA splicing as component of the spliceosome. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Identified in the spliceosome C complex. Component of the PRP19-CDC5L splicing complex composed of a core complex comprising a homotetramer of PRPF19, CDC5L, PLRG1 and BCAS2, and at least three less stably associated proteins CTNNBL1, CWC15 and HSPA8. Interacts (via its WD40 repeat domain) directly with CDC5L (via its C-terminal); the interaction is required for mRNA splicing but not for spliceosome assembly. Component of the minor spliceosome, which splices U12-type introns. Within this complex, interacts with CRIPT. Also interacts directly in the complex with BCAS2 and PRPF19. Interacts with USB1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the WD repeat PRL1/PRL2 family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001203">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F7A5V7"/>
    <dbReference type="FunCoup" id="F7A5V7">
        <property type="interactions" value="413"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000018919"/>
    <dbReference type="Ensembl" id="ENSCINT00000018919.3">
        <property type="protein sequence ID" value="ENSCINP00000018919.3"/>
        <property type="gene ID" value="ENSCING00000009307.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155316"/>
    <dbReference type="HOGENOM" id="CLU_000288_72_2_1"/>
    <dbReference type="InParanoid" id="F7A5V7"/>
    <dbReference type="OMA" id="FAMCFDQ"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000012">
        <property type="entry name" value="Putative pleiotropic regulator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045241">
        <property type="entry name" value="Prp46/PLRG1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19923:SF0">
        <property type="entry name" value="PLEIOTROPIC REGULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19923">
        <property type="entry name" value="WD40 REPEAT PROTEINPRL1/PRL2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 7" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="187"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="229"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="271"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="313"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="396"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00046238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00062641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00073631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000018919.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="501" mass="55915" checksum="FC5B1CB7F5BA6199" modified="2012-04-18" version="2">MADVAQDITKHSMHTLVFRSLKRSHDMFISDQAAPVPNNDAASKLSRSVKTVSEYGPVLHMAKDKKKTAKITSATNGTQGIYYIYICLILDLNFWLKWCFNTNSHKLVFLGDSSMLAIMDSNQKDISNGTAKAKSSANVTVNPDDIQRLHETNKALLAVNSRPIAPRKAQIMPKPRWHAPWKLYRVISGHLGWVRSVAFEPGNEWFVTGSNDRTIKIWDLASGKLKLSLTGHISSVRGLAVSARNPYLFSCGEDKMVKCWDLEQNKVIRHYHGHLSACYALDLHPTIDVLVTCGRDSSARVWDMRTKANIMTLSGHTNTVADVKCQAALPEVITASHDCTVRLWDLAAGKTRSCLTNHKKSIRSIAMHPTQYTFASGAPDNIKQWKFPDGDFIQNMSGHNAIVNCLALSSDGVLVSGADNGTVHLWDWRTGYNFQRTKAPVQPGSLDSESGVFAMAFDKSESRLVTCEADKTIKIYKEDDEASEESHPINWKPEIVRKKRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>F7ADC2</accession>
    <name>F7ADC2_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ceramide-1-phosphate transfer protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100181489</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GLTP family.</text>
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    <dbReference type="AlphaFoldDB" id="F7ADC2"/>
    <dbReference type="FunCoup" id="F7ADC2">
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    <dbReference type="STRING" id="7719.ENSCINP00000007561"/>
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        <property type="protein sequence ID" value="ENSCINP00000007561.3"/>
        <property type="gene ID" value="ENSCING00000003673.3"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
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        <property type="term" value="F:ceramide 1-phosphate binding"/>
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        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:1902388">
        <property type="term" value="F:ceramide 1-phosphate transfer activity"/>
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    <dbReference type="GO" id="GO:0035627">
        <property type="term" value="P:ceramide transport"/>
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        <property type="term" value="P:intermembrane lipid transfer"/>
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    <dbReference type="GO" id="GO:0032691">
        <property type="term" value="P:negative regulation of interleukin-1 beta production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="Ceramide-1-phosphate transfer protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3520.10">
        <property type="entry name" value="Glycolipid transfer protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036497">
        <property type="entry name" value="GLTP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014830">
        <property type="entry name" value="Glycolipid_transfer_prot_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10219:SF43">
        <property type="entry name" value="GLYCOLIPID TRANSFER PROTEIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10219">
        <property type="entry name" value="GLYCOLIPID TRANSFER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08718">
        <property type="entry name" value="GLTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110004">
        <property type="entry name" value="Glycolipid transfer protein, GLTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycolipid transfer protein" evidence="2">
        <location>
            <begin position="35"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000007561.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="226" mass="25825" checksum="7E41A57ADF729563" modified="2012-04-18" version="2">MSKSSVECPQTAAFSVMKMSKLFQECATSPKARQVDMDRYLDAWDELIRFLNSMGKAFTFVSSDVVEKVGILRDFRKSSNKEHYETIEKMILFEKENKLVNFKSAPSKTVTAYGCRTLLRLHRALKFLLILIGKLAHNEDEGKVSLMGYNAYHASPMAKYHPWIVQKAVGIAVYMLPDRTTFLKKMCQDLSDEEMAKTMGVCSESMDAVYEFTNALYSKYDLTEIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>F7ATR6</accession>
    <accession>A0A1W2WMI1</accession>
    <name>F7ATR6_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized LOC100179368</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC100179368</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01002742">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002130266.1">
        <property type="nucleotide sequence ID" value="XM_002130230.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F7ATR6"/>
    <dbReference type="Ensembl" id="ENSCINT00000011629.3">
        <property type="protein sequence ID" value="ENSCINP00000011629.3"/>
        <property type="gene ID" value="ENSCING00000005611.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100179368"/>
    <dbReference type="KEGG" id="cin:100179368"/>
    <dbReference type="GeneTree" id="ENSGT00390000002614"/>
    <dbReference type="HOGENOM" id="CLU_1408287_0_0_1"/>
    <dbReference type="InParanoid" id="F7ATR6"/>
    <dbReference type="OMA" id="TWFMAVR"/>
    <dbReference type="OrthoDB" id="10396837at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009545">
        <property type="entry name" value="Claudin-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06653">
        <property type="entry name" value="Claudin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="107"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="157"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000011629.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="193" mass="20815" checksum="36FDA79EC35CA3E2" modified="2011-07-27" version="1">MDRGRKPFVAALFAGALALILAVIGIATPEWTVARGNKIGLWTLTDENGVSHSWADHSYTEEFFYATWFMAVRGMMMAGVIIGSLGLLFNVFVVAKRFQTTSYGNILLSYYCLAFACLIVSVCVYSALICQPVNSSGVIIDNNAAAVEFVYSAGYGYSIWLTWLGAGIFLPAAGLAYEGGHQDALGKNLLIAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>F7AZQ8</accession>
    <name>F7AZQ8_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cadherin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Postsynaptic cell membrane</location>
            <topology evidence="10">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the calsyntenin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="F7AZQ8"/>
    <dbReference type="FunCoup" id="F7AZQ8">
        <property type="interactions" value="15"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000002052"/>
    <dbReference type="Ensembl" id="ENSCINT00000002052.3">
        <property type="protein sequence ID" value="ENSCINP00000002052.3"/>
        <property type="gene ID" value="ENSCING00000001093.3"/>
    </dbReference>
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        <name type="scientific">Ciona intestinalis</name>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
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                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <text evidence="1">Homodimer.</text>
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</entry>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
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                <person name="Haga S."/>
                <person name="Hayashi H."/>
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                <person name="Inaba K."/>
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                <person name="Kobayashi K."/>
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                <person name="Manohar C."/>
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                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
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                <person name="Nomoto H."/>
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                <person name="Rigoutsos I."/>
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                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
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            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
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                <person name="Inaba K."/>
            </authorList>
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            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">wild type</strain>
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    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <feature type="transmembrane region" description="Helical" evidence="1">
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            <begin position="166"/>
            <end position="183"/>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    </evidence>
    <sequence length="188" mass="21446" checksum="E9168307400F580A" modified="2012-04-18" version="2">MPLNRQRIFVHCFMSVCDPDIEGNCNVTGCATSGRRRRSTEMEKSTSKVITMGPINVETYSIQKAALKKSSDSGITADDLKINGLVKEPKIERFDSSQLIKAYCEKMECEYKCQMNRAGQLMCICPNNFYVDNDGKACVRSKYFSYRFPNLARHGEFLQSYSHSNILVLAFAVCFIVLLLKFINRNYI</sequence>
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    <accession>F7BIB7</accession>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
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                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
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                <person name="Nomoto H."/>
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                <person name="Suzuki M.M."/>
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                <person name="Yoshizaki F."/>
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                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="7" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">wild type</strain>
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    <reference evidence="7" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome 3"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
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        <property type="entry name" value="Myosin light chain kinase isoform-III"/>
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        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
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        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
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    </dbReference>
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        <property type="entry name" value="Kinase-like_dom_sf"/>
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        <property type="entry name" value="Prot_kinase_dom"/>
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    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
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    <dbReference type="PANTHER" id="PTHR24342:SF20">
        <property type="entry name" value="MYOSIN LIGHT CHAIN KINASE, SMOOTH MUSCLE"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR24342">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE 17"/>
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    </dbReference>
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        <property type="match status" value="1"/>
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    </evidence>
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        <source>
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        </source>
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    <reference evidence="10" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="De Tomaso A."/>
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                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
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                <person name="Hastings K.E."/>
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                <person name="Huang W."/>
                <person name="Kawashima T."/>
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                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
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                <person name="Larimer F."/>
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                <person name="Lucas S."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dynein intermediate chain family.</text>
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    <dbReference type="OMA" id="MHDRPEY"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005868">
        <property type="term" value="C:cytoplasmic dynein complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045504">
        <property type="term" value="F:dynein heavy chain binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045503">
        <property type="term" value="F:dynein light chain binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010970">
        <property type="term" value="P:transport along microtubule"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001896">
        <property type="entry name" value="Cytoplasmic dynein 1 intermediate chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000026">
        <property type="entry name" value="cytoplasmic dynein 1 intermediate chain 2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025956">
        <property type="entry name" value="DYNC1I1/DYNC1I2"/>
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    <dbReference type="InterPro" id="IPR050687">
        <property type="entry name" value="Dynein_IC"/>
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    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12442:SF22">
        <property type="entry name" value="CYTOPLASMIC DYNEIN 1 INTERMEDIATE CHAIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12442">
        <property type="entry name" value="DYNEIN INTERMEDIATE CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11540">
        <property type="entry name" value="Dynein_IC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 7" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="360"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="410"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="45"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="67"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000008987.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="528" mass="59431" checksum="C21930E5F4E57212" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>H2XJM2</accession>
    <accession>A0A1W2WGI1</accession>
    <name>H2XJM2_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Proline-rich nuclear receptor coactivator 2 B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100177436</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_002127241.1">
        <property type="nucleotide sequence ID" value="XM_002127205.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XJM2"/>
    <dbReference type="Ensembl" id="ENSCINT00000037290.1">
        <property type="protein sequence ID" value="ENSCINP00000029854.1"/>
        <property type="gene ID" value="ENSCING00000019348.1"/>
    </dbReference>
    <dbReference type="GeneID" id="100177436"/>
    <dbReference type="KEGG" id="cin:100177436"/>
    <dbReference type="GeneTree" id="ENSGT00530000063881"/>
    <dbReference type="HOGENOM" id="CLU_1885037_0_0_1"/>
    <dbReference type="InParanoid" id="H2XJM2"/>
    <dbReference type="OMA" id="TPSPNKE"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016071">
        <property type="term" value="P:mRNA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028322">
        <property type="entry name" value="PNRC-like_rgn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026780">
        <property type="entry name" value="PNRC1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15405">
        <property type="entry name" value="PROLINE-RICH NUCLEAR RECEPTOR COACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15365">
        <property type="entry name" value="PNRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0010">Activator</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="25"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="83"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000029854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="137" mass="15002" checksum="8DD3BF98EF4D8079" modified="2012-04-18" version="1">MAILEKCPPACLERLGLTGADAVSKNPKCQVVRPKQRTGRKTPSPNKEIRNSQPNSPLTPFWASHQQFTCHVPISPNHGLFNSGSYAGARFHSPPTADALPKPPQHWVNVPSCAQQMQSQQLSELSTRLKGMLKVPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>H2XKP7</accession>
    <accession>A0A1W2WGL0</accession>
    <name>H2XKP7_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cation efflux protein transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Zinc ion transporter mediating zinc entry from the cytosol into the lumen of organelles along the secretory pathway. By contributing to zinc ion homeostasis within the early secretory pathway, regulates the activation and folding of enzymes like alkaline phosphatases.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>Zn(2+)(in) = Zn(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29351"/>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homooligomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
            <location evidence="2">trans-Golgi network</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. SLC30A subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="H2XKP7"/>
    <dbReference type="FunCoup" id="H2XKP7">
        <property type="interactions" value="126"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000030229"/>
    <dbReference type="Ensembl" id="ENSCINT00000032875.1">
        <property type="protein sequence ID" value="ENSCINP00000030229.1"/>
        <property type="gene ID" value="ENSCING00000024330.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159571"/>
    <dbReference type="HOGENOM" id="CLU_013430_0_3_1"/>
    <dbReference type="InParanoid" id="H2XKP7"/>
    <dbReference type="OMA" id="KWRANER"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
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                <person name="Nomoto H."/>
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                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
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            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
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                <person name="Sasaki A."/>
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                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
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            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
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    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
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                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
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                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
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                <person name="Branno M."/>
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                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
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                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
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                <person name="Shin-i T."/>
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                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
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                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
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                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
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                <person name="Wiley G.B."/>
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                <person name="Zeller R.W."/>
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            <strain evidence="1">wild type</strain>
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            <authorList>
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        <scope>IDENTIFICATION</scope>
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    </evidence>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
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                <person name="Huang W."/>
                <person name="Kawashima T."/>
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                <person name="Martinez D."/>
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                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="18">wild type</strain>
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    </reference>
    <reference evidence="18" key="3">
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        <scope>IDENTIFICATION</scope>
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    <reference evidence="18" key="4">
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            <authorList>
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            <text>S-[NEDD8-protein]-yl-[E2 NEDD8-conjugating enzyme]-L-cysteine + [cullin]-L-lysine = [E2 NEDD8-conjugating enzyme]-L-cysteine + N(6)-[NEDD8-protein]-yl-[cullin]-L-lysine.</text>
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    <comment type="pathway">
        <text evidence="4">Protein modification; protein neddylation.</text>
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    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
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                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
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                <person name="Nomoto H."/>
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                <person name="Sasaki A."/>
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                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
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    <feature type="transmembrane region" description="Helical" evidence="1">
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            <begin position="58"/>
            <end position="75"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
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            <dbReference type="Ensembl" id="ENSCINP00000031398.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
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    <reference evidence="5" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
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                <person name="DeSantis R."/>
                <person name="Doyle S."/>
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                <person name="Imai K.S."/>
                <person name="Inaba K."/>
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                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
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                <person name="Medina M."/>
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                <person name="Mount S."/>
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                <person name="Miura S."/>
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                <person name="Oishi K."/>
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                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">wild type</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="4">
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        <source>
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    </evidence>
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        <source>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
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                <person name="Imai K.S."/>
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                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
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                <person name="Miura S."/>
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                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
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            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <dbReference type="STRING" id="7719.ENSCINP00000032190"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
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                <person name="Putnam N."/>
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                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
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                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
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                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
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            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1">wild type</strain>
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    <reference evidence="1" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <source>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
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                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
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                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
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                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
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                <person name="Lemaire P."/>
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                <person name="Meinertzhagen I.A."/>
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                <person name="Terry A."/>
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                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
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                <person name="Richardson P."/>
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                <person name="Sasakura Y."/>
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                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
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                <person name="Wiley G.B."/>
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                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
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            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002125150.1">
        <property type="nucleotide sequence ID" value="XM_002125114.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XW40"/>
    <dbReference type="Ensembl" id="ENSCINT00000034957.1">
        <property type="protein sequence ID" value="ENSCINP00000033874.1"/>
        <property type="gene ID" value="ENSCING00000018469.1"/>
    </dbReference>
    <dbReference type="GeneID" id="100185812"/>
    <dbReference type="KEGG" id="cin:100185812"/>
    <dbReference type="GeneTree" id="ENSGT00660000097255"/>
    <dbReference type="HOGENOM" id="CLU_1721713_0_0_1"/>
    <dbReference type="InParanoid" id="H2XW40"/>
    <dbReference type="OMA" id="EYWTADG"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003582">
        <property type="entry name" value="ShKT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01549">
        <property type="entry name" value="ShK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51670">
        <property type="entry name" value="SHKT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014093523" evidence="2">
        <location>
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        </location>
    </feature>
    <feature type="domain" description="ShKT" evidence="3">
        <location>
            <begin position="55"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000033874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="152" mass="17396" checksum="D316CAFA8AD16588" modified="2012-04-18" version="1">MKLFVMIMCVNYHIFVAESSKVNSNNDQVEKSPKFSKDVVFPPVPPEYWTADGECADNPTFVEDCPVWAEMGFCDNEEKGKQYVKWMRKHCMLSCKICEPTSQPLKIALKLCAGVLVLIWLAGFIRFPRVYSSRKCFKLLVSMGLATCIYFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>H2XW91</accession>
    <name>H2XW91_CIOIN</name>
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        <recommendedName>
            <fullName evidence="4">Prolyl 4-hydroxylase alpha subunit Fe(2+) 2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">wild type</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01000387">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XW91"/>
    <dbReference type="STRING" id="7719.ENSCINP00000033925"/>
    <dbReference type="Ensembl" id="ENSCINT00000035823.1">
        <property type="protein sequence ID" value="ENSCINP00000033925.1"/>
        <property type="gene ID" value="ENSCING00000022764.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163795"/>
    <dbReference type="HOGENOM" id="CLU_176518_0_0_1"/>
    <dbReference type="InParanoid" id="H2XW91"/>
    <dbReference type="OMA" id="KWIHAND"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031418">
        <property type="term" value="F:L-ascorbic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004656">
        <property type="term" value="F:procollagen-proline 4-dioxygenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.620:FF:000066">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045054">
        <property type="entry name" value="P4HA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044862">
        <property type="entry name" value="Pro_4_hyd_alph_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10869">
        <property type="entry name" value="PROLYL 4-HYDROXYLASE ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10869:SF244">
        <property type="entry name" value="PROLYL 4-HYDROXYLASE SUBUNIT ALPHA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13640">
        <property type="entry name" value="2OG-FeII_Oxy_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0847">Vitamin C</keyword>
    <feature type="domain" description="Prolyl 4-hydroxylase alpha subunit Fe(2+) 2OG dioxygenase" evidence="4">
        <location>
            <begin position="3"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000033925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="96" mass="11051" checksum="541B4E83DA75CC80" modified="2012-04-18" version="1">MERRIATFLTYFSDVEYGGNTPFVYQEVVAEPIKGSAIFWYDVFNDGSADERTEHAACPVVLGNKWAGNLWLTFGDQTFTKQCPLNNKTPREYRIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>H2XWR9</accession>
    <name>H2XWR9_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Probable D-lactate dehydrogenase, mitochondrial</fullName>
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        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100186045</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">wild type</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Involved in D-lactate, but not L-lactate catabolic process.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FAD-binding oxidoreductase/transferase type 4 family.</text>
    </comment>
    <dbReference type="EC" id="1.1.2.4" evidence="9"/>
    <dbReference type="EMBL" id="EAAA01002282">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XWR9"/>
    <dbReference type="FunCoup" id="H2XWR9">
        <property type="interactions" value="53"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000034103"/>
    <dbReference type="Ensembl" id="ENSCINT00000037045.1">
        <property type="protein sequence ID" value="ENSCINP00000034103.1"/>
        <property type="gene ID" value="ENSCING00000023597.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158705"/>
    <dbReference type="HOGENOM" id="CLU_017779_3_0_1"/>
    <dbReference type="InParanoid" id="H2XWR9"/>
    <dbReference type="OMA" id="GQGFEWA"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004458">
        <property type="term" value="F:D-lactate dehydrogenase (cytochrome) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008720">
        <property type="term" value="F:D-lactate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903457">
        <property type="term" value="P:lactate catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.45.10:FF:000001">
        <property type="entry name" value="D-lactate dehydrogenase mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2740:FF:000001">
        <property type="entry name" value="D-lactate dehydrogenase mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.465.10:FF:000030">
        <property type="entry name" value="probable D-lactate dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.45.10">
        <property type="entry name" value="Vanillyl-alcohol Oxidase, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004113">
        <property type="entry name" value="FAD-bd_oxidored_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016164">
        <property type="entry name" value="FAD-linked_Oxase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016171">
        <property type="entry name" value="Vanillyl_alc_oxidase_C-sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11748">
        <property type="entry name" value="D-LACTATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11748:SF111">
        <property type="entry name" value="D-LACTATE DEHYDROGENASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02913">
        <property type="entry name" value="FAD-oxidase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55103">
        <property type="entry name" value="FAD-linked oxidases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="12">
        <location>
            <begin position="45"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00053432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00072812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000034103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="470" mass="50547" checksum="61E8AEECE2A89FEB" modified="2012-04-18" version="1">FKRYKSISPSIDKLHSELSAIVGKKNAVSSLAAREHHNHDESHHTGGSPAIVVWPNSVQEVSEVARACYLNSVPMIPFGTGTGLEGGVIPKTESVTVNLTNMDAISDLNIEDFDVRVQPGVTRKALNREIRNTGLWFPVDPGADASICGMVATGASGTNAVRYGTIKHNVLNLEVVLADGTILNTGGSKRRCRKSSAGYNLTELFIGSEGTLGFITSATVRLHGMPESTLSAVCSFPSVQDAVNTSIQVLQYSIPIARIEFLDNVAIKAVNNFSKLENKIAPTLFLEFHGSEAGAKEQVNQVAEIAAANGGTQFKWAETVEERSKLWQARHDTFYALLALKPGNKGYSTDVCVPISQLPAAIEHSLSIVEKYGILCGIAGHVGDGNYHCLLMLDSDNESEVLRAKMAAEEIGRKAIFLGGSCTGEHGIGIGKRELLKEEVGLETLNVMKGIKNVLDPRNLMNPFKGLPEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>H2XX29</accession>
    <name>H2XX29_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="H2XX29"/>
    <dbReference type="STRING" id="7719.ENSCINP00000034213"/>
    <dbReference type="Ensembl" id="ENSCINT00000033402.1">
        <property type="protein sequence ID" value="ENSCINP00000034213.1"/>
        <property type="gene ID" value="ENSCING00000022322.1"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1237470_0_0_1"/>
    <dbReference type="InParanoid" id="H2XX29"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051022">
        <property type="entry name" value="Notch_Cell-Fate_Det"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24049">
        <property type="entry name" value="CRUMBS FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1 4" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="124"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="32"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="59"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000034213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="224" mass="24521" checksum="416754A839F8367E" modified="2012-04-18" version="1">MPGCRGNDVCASNPCPHDQYCNDIWEKFECIPLGGCASSPCLNQASCIPFNNGSYACSCSTYYMGNLCETYIPCLMVDCSTKGTNQVCVSNGLPGGTTCACKSGYFDLNDFCVPVSVATAGIGWWAYVVIGLVGLLVFALAIWCVRRRYFNLEQKGENGNLQNRKKLQTITPKLNGNNTNGRNNSSYEFDTITRTQLPSLPRPDILSEDVLIVDEEAFSNYAEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>H2XYG3</accession>
    <name>H2XYG3_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Complex I-39kD</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">NADH-ubiquinone oxidoreductase 39 kDa subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100176100</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="5">Complex I is composed of 45 different subunits. This a component of the hydrophobic protein fraction. Interacts with BLOC1S1. Interacts with SLC2A4. Interacts with CLOCK. Interacts with RAB5IF.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I NDUFA9 subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="H2XYG3"/>
    <dbReference type="FunCoup" id="H2XYG3">
        <property type="interactions" value="795"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000034697"/>
    <dbReference type="Ensembl" id="ENSCINT00000034253.1">
        <property type="protein sequence ID" value="ENSCINP00000034697.1"/>
        <property type="gene ID" value="ENSCING00000020573.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000006865"/>
    <dbReference type="HOGENOM" id="CLU_007383_6_4_1"/>
    <dbReference type="InParanoid" id="H2XYG3"/>
    <dbReference type="OMA" id="VHDSYLP"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd05271">
        <property type="entry name" value="NDUFA9_like_SDR_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051207">
        <property type="entry name" value="ComplexI_NDUFA9_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12126:SF11">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 9, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12126">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE 39 KDA SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00040720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00042000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00043145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00046455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000034697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="383" mass="43099" checksum="02A54CD9E6A87051" modified="2012-04-18" version="1">MRGLLICHSNSQALARLCIRPCTSFAQSRGVHDSYLPSGRGGRSSFSGVVATVFGPTGFIGLSVVNHLAAIGSSLVLPYRGTFERVQRHKLMSDLGQMHVREMFNSNASDDRVRELIEHSNVVVNLIGSTKPYRFYSMEEVNVTWPTRLAQLVAEKNDGTRLIHLSRLNCHSDEARKHSEILKQNFEAEQNLKEIYPGSTIVRCSSVHGKYDKFTTFWLSPRNLILFLPPGWVSLGGESTIIQPVCVSDVAEGVARLARHPDAPGQTFEFVGPDRFTLNEFLEYVYNCLGRDYYPEIVKLVLFLKKKHFKSLNNSHHNNITTTIMTSQMQTTDRTTGAPGLLELGISPVAYEEKRDNIIRKYVTNPLLHQVNEESQPMPTVQH</sequence>
</entry>
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    <accession>H2XYH2</accession>
    <name>H2XYH2_CIOIN</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">wild type</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01002897">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XYH2"/>
    <dbReference type="Ensembl" id="ENSCINT00000031316.1">
        <property type="protein sequence ID" value="ENSCINP00000034706.1"/>
        <property type="gene ID" value="ENSCING00000020935.1"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3359383_0_0_1"/>
    <dbReference type="InParanoid" id="H2XYH2"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000034706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="36" mass="4253" checksum="30E76CF208424510" modified="2012-04-18" version="1">MTFLFHIIKSNPPSQFIHVFHVIINHPILVKNNNQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>H2XZ09</accession>
    <name>H2XZ09_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">LamG-like jellyroll fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
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            <taxon>Metazoa</taxon>
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    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
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                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
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                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="H2XZ09"/>
    <dbReference type="Ensembl" id="ENSCINT00000034285.1">
        <property type="protein sequence ID" value="ENSCINP00000034893.1"/>
        <property type="gene ID" value="ENSCING00000023116.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000000513"/>
    <dbReference type="HOGENOM" id="CLU_067110_0_0_1"/>
    <dbReference type="InParanoid" id="H2XZ09"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006558">
        <property type="entry name" value="LamG-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13385">
        <property type="entry name" value="Laminin_G_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00560">
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        <location>
            <begin position="1"/>
            <end position="16"/>
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        <source>
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        <source>
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    <evidence type="ECO:0000313" key="8">
        <source>
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    </evidence>
    <sequence length="354" mass="37899" checksum="63A35FDB95EA6410" modified="2012-04-18" version="1">MLEFVLVVSFVGLVSCQDPNVYWSMDKTLGNQITPDIGNVTIFTFNTSGSFVNGYNGNALRLYQTSDTFNGVGAVADECVFEPANQCNGGLTAAAWLRPHAWSASARLFGTGTSILSIVGFAIRVRSSFLTRELYGEVVLINGTLSTATIDLDLVPLDTWTHLTMVWHETTGIKMYINGTEPTYISPSASSSVSGLLSSLQHFTFGVSSQLELDFDEVKLWYNPLTPTQVMRAVTNVPTTTTTDSMQTTPQTTTTDSMQTTPQTTTTDSMQTVGQTATTNTLQTTPPIIIVNGRACDSLCLGLAIGLPLGLMLIVLVVVCCCCCRNSNGCCACCCGGAGGDDDDEKERETDTAL</sequence>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    <dbReference type="Ensembl" id="ENSCINT00000032788.1">
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    </evidence>
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        <source>
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    </evidence>
    <sequence length="220" mass="25395" checksum="136939FA3CB52F0B" modified="2012-04-18" version="1">MKRMNWNQMKSNAIQEKSFWVSAKEEIFESPDLFTRLMATFGQKKVTKKAVEVEKPQKKVKELKVLDSKSAQNLSIFLGTLKVPYAQVKTMILGVTDDMDESLINNLLKQLPEQEMISAVSEYKKQYDDLVEAEQFLCTISEIKRLHPRLQHIRFIRQFDEMVSDIKPDIVAVTAACQDIMKSNKFKKFLELVLLIGNYMNSGSRNAQTLAFDISYLTKV</sequence>
</entry>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
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                <person name="Huang W."/>
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                <person name="Meinertzhagen I.A."/>
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                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
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                <person name="Imai K.S."/>
                <person name="Inaba K."/>
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                <person name="Kobayashi K."/>
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                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">wild type</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XZZ9"/>
    <dbReference type="Ensembl" id="ENSCINT00000035460.1">
        <property type="protein sequence ID" value="ENSCINP00000035233.1"/>
        <property type="gene ID" value="ENSCING00000022713.1"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2793220_0_0_1"/>
    <dbReference type="InParanoid" id="H2XZZ9"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000035233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="68" mass="8376" checksum="3F2BC7A995585620" modified="2012-04-18" version="1">MSYNYVYIYTGKHPRMLCTVANKICRELPTKRRRPGRENRTVRSSVYFWLSEMKYIIGFRKKFQVFLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>H2Y1D1</accession>
    <name>H2Y1D1_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
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                <person name="Chapman J."/>
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                <person name="De Tomaso A."/>
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                <person name="Hastings K.E."/>
                <person name="Ho I."/>
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                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
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                <person name="Meinertzhagen I.A."/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
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                <person name="Goodstein D.M."/>
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                <person name="Ho I."/>
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                <person name="Huang W."/>
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                <person name="Mineta K."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">UTP--glucose-1-phosphate uridylyltransferase catalyzing the conversion of glucose-1-phosphate into UDP-glucose, a crucial precursor for the production of glycogen.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>alpha-D-glucose 1-phosphate + UTP + H(+) = UDP-alpha-D-glucose + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19889"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.7.7.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:19890"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homooctamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UDPGP type 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.9" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="L7N0T4"/>
    <dbReference type="STRING" id="7719.ENSCINP00000015627"/>
    <dbReference type="Ensembl" id="ENSCINT00000015627.3">
        <property type="protein sequence ID" value="ENSCINP00000015627.3"/>
        <property type="gene ID" value="ENSCING00000007614.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153464"/>
    <dbReference type="HOGENOM" id="CLU_023632_2_0_1"/>
    <dbReference type="InParanoid" id="L7N0T4"/>
    <dbReference type="OMA" id="VECCMEV"/>
    <dbReference type="UniPathway" id="UPA00164"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003983">
        <property type="term" value="F:UTP:glucose-1-phosphate uridylyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005978">
        <property type="term" value="P:glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005977">
        <property type="term" value="P:glycogen metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006011">
        <property type="term" value="P:UDP-alpha-D-glucose metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.10.10:FF:000001">
        <property type="entry name" value="UTP--glucose-1-phosphate uridylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002618">
        <property type="entry name" value="UDPGP_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016267">
        <property type="entry name" value="UDPGP_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43511">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01704">
        <property type="entry name" value="UDPGP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000015627.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="237" mass="26456" checksum="E5BA7DE51815A35B" modified="2013-03-06" version="1">SKPCDFVMEVTDKTRADVKGGTLIEYENKLRLLEIAQVPKEHVDEFKSVSKFKIFNTNNLWMSMQSISELTTAGKMEMEVIVNNKRLANGTNVIQLETASGAGIKNFHNAIGINVPRSRFLPVKKTSDLLLVMSNLYELKNGSMVMSSKRQFPTTPLIKLGDEHFSKIHKFLGRFANIPDMLELDHLTVSGDVTFGKDVTLKGTVIIIANHGERIDIPSGAFLENKIVSGNLRILDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-12-07" modified="2026-01-28" version="114" xmlns="https://uniprot.org/uniprot">
    <accession>Q5VHU5</accession>
    <accession>A0A1W3JLU9</accession>
    <accession>F6SKQ3</accession>
    <name>Q5VHU5_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ammonium transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">amt-1</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2003-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Huang C.-H."/>
                <person name="Chen Y."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">wild type</strain>
        </source>
    </reference>
    <reference evidence="12" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ammonia transporter channel (TC 1.A.11.2) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001404">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AY455820">
        <property type="protein sequence ID" value="AAS80045.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009857502.1">
        <property type="nucleotide sequence ID" value="XM_009859200.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q5VHU5"/>
    <dbReference type="STRING" id="7719.ENSCINP00000008302"/>
    <dbReference type="Ensembl" id="ENSCINT00000008302.3">
        <property type="protein sequence ID" value="ENSCINP00000008302.3"/>
        <property type="gene ID" value="ENSCING00000004026.3"/>
    </dbReference>
    <dbReference type="GeneID" id="493859"/>
    <dbReference type="CTD" id="180729"/>
    <dbReference type="GeneTree" id="ENSGT00530000064546"/>
    <dbReference type="HOGENOM" id="CLU_000445_33_1_1"/>
    <dbReference type="OMA" id="GCDFKFS"/>
    <dbReference type="OrthoDB" id="534912at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008519">
        <property type="term" value="F:ammonium channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097272">
        <property type="term" value="P:ammonium homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072488">
        <property type="term" value="P:ammonium transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3430.10:FF:000010">
        <property type="entry name" value="Ammonium transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3430.10">
        <property type="entry name" value="Ammonium transporter AmtB like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029020">
        <property type="entry name" value="Ammonium/urea_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001905">
        <property type="entry name" value="Ammonium_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024041">
        <property type="entry name" value="NH4_transpt_AmtB-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00836">
        <property type="entry name" value="amt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11730">
        <property type="entry name" value="AMMONIUM TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11730:SF6">
        <property type="entry name" value="AMMONIUM TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00909">
        <property type="entry name" value="Ammonium_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111352">
        <property type="entry name" value="Ammonium transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="7 8" id="KW-0924">Ammonia transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="26"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="66"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="127"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="153"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="235"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="303"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="327"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="362"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="406"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="domain" description="Ammonium transporter AmtB-like" evidence="10">
        <location>
            <begin position="27"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="498"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="522"/>
            <end position="531"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AAS80045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000008302.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="531" mass="56848" checksum="AD4C9EB93E943CEF" modified="2004-12-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2008-01-15" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>A8WHA5</accession>
    <accession>A0A1W2VSV2</accession>
    <name>A8WHA5_CIOIN</name>
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        <submittedName>
            <fullName evidence="1 2">Class I cytokine receptor like factor-3</fullName>
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    </protein>
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        <name evidence="1 2" type="primary">clf-3</name>
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    <organism evidence="1">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
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            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="journal article" date="2007" name="BMC Evol. Biol." volume="7" first="120" last="120">
            <title>Evolution of Class I cytokine receptors.</title>
            <authorList>
                <person name="Liongue C."/>
                <person name="Ward A.C."/>
            </authorList>
            <dbReference type="PubMed" id="17640376"/>
            <dbReference type="DOI" id="10.1186/1471-2148-7-120"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="BN000968">
        <property type="protein sequence ID" value="CAL08000.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001107600.1">
        <property type="nucleotide sequence ID" value="NM_001114128.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A8WHA5"/>
    <dbReference type="STRING" id="7719.ENSCINP00000033844"/>
    <dbReference type="Ensembl" id="ENSCINT00000036882.1">
        <property type="protein sequence ID" value="ENSCINP00000033844.1"/>
        <property type="gene ID" value="ENSCING00000021539.1"/>
    </dbReference>
    <dbReference type="GeneID" id="100135650"/>
    <dbReference type="KEGG" id="cin:100135650"/>
    <dbReference type="CTD" id="100135650"/>
    <dbReference type="GeneTree" id="ENSGT00940000153469"/>
    <dbReference type="HOGENOM" id="CLU_057983_0_0_1"/>
    <dbReference type="OMA" id="HEWTPGF"/>
    <dbReference type="OrthoDB" id="9984427at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
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    </dbReference>
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        <property type="term" value="F:DNA clamp unloader activity"/>
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    </dbReference>
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        <property type="entry name" value="Immunoglobulins"/>
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    </dbReference>
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        <property type="entry name" value="Fibronectin type III"/>
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    </dbReference>
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    <keyword evidence="1" id="KW-0675">Receptor</keyword>
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        <source>
            <dbReference type="EMBL" id="CAL08000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000033844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="463" mass="51843" checksum="085DD675BE07F59A" modified="2008-01-15" version="1">MYEGVQEAIETVDSARQHKCELESREKHLKTAVCQVSATAEHARKEIKHHFESMNKEIACKIDLRKKSLLHEITLIEKTALIPLNECQHFLQERISEAGTLVSTGENILQCDYDNTSGKINFDSFISMAENLSLDSVPEVPLLSDVPSISFVFNEDFSSEICQALEHYGTVSKRSPVQISDLKPIPGGVVVTWSDNNLGGNEKSHRIYKLQLCHGRVNLPLQLKNTNETIFKDSYVGPNLTYSVKNLSSNSIYTFRVCQCSLMVNGDTIKQCSPWSVYQEKMTTMDPFLWSNSSDSVSYTLSDKGRSAIKKNSLDTVLYSARSSHIIGYSINFKIDVEGKSYHTNDCLALTVRRDADACSLHMKSGTLCLMADGHVWVNGTKSTTRFSHFCRGSVVAFDVSHIPSTEFGYIGKEIKQLRVSITVGEHCAVFNWHPLKAAAQLHSQDFAFAMLFRTPGWKVSIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>F6PLM3</accession>
    <name>F6PLM3_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Bromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
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            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="F6PLM3"/>
    <dbReference type="STRING" id="7719.ENSCINP00000009891"/>
    <dbReference type="Ensembl" id="ENSCINT00000009891.3">
        <property type="protein sequence ID" value="ENSCINP00000009891.3"/>
        <property type="gene ID" value="ENSCING00000017426.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000067985"/>
    <dbReference type="HOGENOM" id="CLU_943204_0_0_1"/>
    <dbReference type="InParanoid" id="F6PLM3"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140672">
        <property type="term" value="C:ATAC complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010484">
        <property type="term" value="F:histone H3 acetyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Bromodomain"/>
    </dbReference>
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        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037800">
        <property type="entry name" value="GCN5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45750">
        <property type="entry name" value="GH11602P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45750:SF3">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bromo" evidence="4">
        <location>
            <begin position="174"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="140"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="140"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="152"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000009891.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="295" mass="34442" checksum="47BC2F4B07FEB21D" modified="2012-04-18" version="2">MRICLRQNDDELDEEKSDSNSNIGSCDSELADSTFRRMYELELYDMEPPEQIDVLAALTYRVMNTYAVQIHIEDTTEKATTIYKKVLQQKKEKKLYEVPKKKESNEEPSIDPYFVKNPIKAEDVEDEGIDLVSRVKKRKMLSEKHREGGSESGEEEEEEEEEGLVCKRIIEKLIGYQYSELFRHPVDVTIVPDYYDIVTRPISLYEIVGKIDGTNPDVGLPFPRSTRRSSHNNYTYELLMDDMKLLVDNAALYNSPDSYTMKQARRLELSFVRLCKENFDTDELVTRLNSLFKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>F6PTX7</accession>
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        <recommendedName>
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        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
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    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="F6PTX7"/>
    <dbReference type="STRING" id="7719.ENSCINP00000011899"/>
    <dbReference type="Ensembl" id="ENSCINT00000011899.3">
        <property type="protein sequence ID" value="ENSCINP00000011899.3"/>
        <property type="gene ID" value="ENSCING00000005764.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155299"/>
    <dbReference type="HOGENOM" id="CLU_582124_0_0_1"/>
    <dbReference type="InParanoid" id="F6PTX7"/>
    <dbReference type="OMA" id="NSDCHWL"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24251:SF30">
        <property type="entry name" value="MEMBRANE FRIZZLED-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24251">
        <property type="entry name" value="OVOCHYMASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CUB" evidence="4">
        <location>
            <begin position="73"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="4">
        <location>
            <begin position="189"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="4">
        <location>
            <begin position="309"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000011899.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="473" mass="53517" checksum="464D314824B0AA5E" modified="2012-04-18" version="2">MESCCDYILLADGADQRQITRLNLEEINEALTFESTTSELTVTLVTDETQNRQGFMAAIFLVSEQRYPRTGSCGHTSTATETPNFFHSPNYPHDYNPNSDCHWLLVASERYRELQLRVVDLRVEDCCDFIEVRDGRNQNAPVLARLNRRPSNPFTSTHGALYLRLVSDSGNHYRGFNATYMLEPLDRRCGVTIPATVRPSQLKSENYPENYANDVTCSWVLVAPQDQKVQLTITDMEIEACCDYLELFDGEDQRLLRKISFDFISEDLVFTSSTNKLTVRFFTDASVTKRGFMSHFQAVQTSALPVDSCGSTVTVGDFYVEFTSPNYPNNYPPNSDCHWLLLSPDSNRQVTLDLSDARFEPCCDFLEIRDGRSIESPLLVRVENQLPDRRLYMSTGGALYLHFWSDSSGGYRGFTAAYKLEEPELLPYPELCYSTKRAYASGRTAIDVNEIMSCTTLGVNARCLKISATGTIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>F6PW95</accession>
    <accession>A0A1W3JEY4</accession>
    <name>F6PW95_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Paired box protein Pax-6</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">wild type</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the paired homeobox family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002992">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6PW95"/>
    <dbReference type="FunCoup" id="F6PW95">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000013350"/>
    <dbReference type="Ensembl" id="ENSCINT00000013350.3">
        <property type="protein sequence ID" value="ENSCINP00000013350.3"/>
        <property type="gene ID" value="ENSCING00000006503.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165785"/>
    <dbReference type="HOGENOM" id="CLU_042491_0_0_1"/>
    <dbReference type="InParanoid" id="F6PW95"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007423">
        <property type="term" value="P:sensory organ development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090596">
        <property type="term" value="P:sensory organ morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00131">
        <property type="entry name" value="PAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000003">
        <property type="entry name" value="Paired box protein Pax-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000069">
        <property type="entry name" value="Paired box protein Pax-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000516">
        <property type="entry name" value="Transcription factor Toy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043182">
        <property type="entry name" value="PAIRED_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001523">
        <property type="entry name" value="Paired_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043565">
        <property type="entry name" value="PAX_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45636:SF41">
        <property type="entry name" value="PAIRED BOX PROTEIN PAX-6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45636">
        <property type="entry name" value="PAIRED BOX PROTEIN PAX-6-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00292">
        <property type="entry name" value="PAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00027">
        <property type="entry name" value="PAIREDBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00351">
        <property type="entry name" value="PAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00034">
        <property type="entry name" value="PAIRED_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51057">
        <property type="entry name" value="PAIRED_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6 11 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 11 12" id="KW-0371">Homeobox</keyword>
    <keyword evidence="9 11 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0563">Paired box</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Paired" evidence="15">
        <location>
            <begin position="26"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="14">
        <location>
            <begin position="229"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="11">
        <location>
            <begin position="231"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="383"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="397"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="409"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022724"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00044108"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
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    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50071"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51057"/>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000013350.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="497" mass="55325" checksum="9084FC539DECC859" modified="2012-04-18" version="2">MATYDSCRQASSMALCFNRESLPHKGHSGMNQLGGMFVNGRPLPDSIRQKIVEFAHNGARPCDISRILQVSNGCVSKILARYYETGTIRPRAIGGSKPRVATPEVVNKIASYKRECPSIFAWEIRDRLLNEGICNNDNIPSVSSINRVLRNLNGDHQTGGFIETTGRDPKSGSQLGLEWSLTCNGWLPQQTMCQEQIAAEGLMGLKGKESVVESEAKDGSSEARLQLKRKLQRNRTSFTQIQVEALEKEFERTHYPDVFARERLATKIDLPEARIQVWFSNRRAKWRREEKIRSQRSSAASDGSDVMRRTNDQQSIGQCGVYSSVTQQHAQANHQLPAYHMPPEPYTGMNGYQMTSLPQTMKVEHFPPMHRMHEAPYLYGLSSGGPSNLTPPPHYTSYDHDRRVEEPSHNTIPTPPSSANQPSPDHDHHGSHMQPLDGGIYQASLSGMHVGHQHHALVGAQATDQSASSMIVPPMTALQNPANFHTAEGYWQSGVQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>F6PXM4</accession>
    <accession>A0A1W2WMV1</accession>
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        <submittedName>
            <fullName evidence="2">Vasohibin-1-like</fullName>
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    </protein>
    <gene>
        <name evidence="2" type="primary">LOC100183377</name>
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    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
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    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_002130450.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6PXM4"/>
    <dbReference type="STRING" id="7719.ENSCINP00000001079"/>
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        <property type="gene ID" value="ENSCING00000000588.3"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="entry name" value="VASH1"/>
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        <property type="entry name" value="VASOHIBIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15750">
        <property type="entry name" value="VASOHIBIN-1-LIKE ISOFORM X2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Vasohibin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <position position="131"/>
        </location>
    </feature>
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        <location>
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        <location>
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        <source>
            <dbReference type="PIRSR" id="PIRSR628131-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000001079.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="319" mass="37258" checksum="64DCD3FF705D07E5" modified="2011-07-27" version="1">MTNKVEALNPDDLEPDDDNVPFYINKNGFPVDDKTWEKMWRYAGKIYPDAKSRMEKIKNNENLNKIPVPTVPVIPARLPTKQCLKLVQNYISSLQYNHTGTQLFEIRKSRPLAGLMDKAKEMIREALPIKCLEAVILAIYLTNGIQNLDRFPIGFKSIFQGHRYYHVVLGVFYNGKYGALGLSRRKDLMDKPLAFKSLFDLIESYNEAYYKYTHQLKKVRIGGIVLHDMCSQEKIQWGILAVGYSHTSYVERQRQFGRYSRDMRSHFNYILPFAPTKDKLRREHTVFPGRSEQLQDIKRTTKSAILNRKRVADEYHVED</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>F6QI37</accession>
    <accession>A0A1W2WP73</accession>
    <name>F6QI37_CIOIN</name>
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        <recommendedName>
            <fullName evidence="5">Developmentally-regulated GTP-binding protein 2</fullName>
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        <alternativeName>
            <fullName evidence="6">Translation factor GTPase DRG2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100179994</name>
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    <organism evidence="9 10">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">wild type</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the conversion of GTP to GDP through hydrolysis of the gamma-phosphate bond in GTP. When hydroxylated at C-3 of 'Lys-21' by JMJD7, may bind to RNA and play a role in translation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Interacts with RWDD1; this interaction confers protection to polyubiquitination and proteolytic degradation. Interacts with JMJD7; this interaction is direct.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001036">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_004226696.4"/>
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        <property type="interactions" value="1143"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000004913"/>
    <dbReference type="Ensembl" id="ENSCINT00000004913.2">
        <property type="protein sequence ID" value="ENSCINP00000004913.2"/>
        <property type="gene ID" value="ENSCING00000002406.2"/>
    </dbReference>
    <dbReference type="GeneID" id="100179994"/>
    <dbReference type="KEGG" id="cin:100179994"/>
    <dbReference type="GeneTree" id="ENSGT00940000153340"/>
    <dbReference type="HOGENOM" id="CLU_044997_0_0_1"/>
    <dbReference type="InParanoid" id="F6QI37"/>
    <dbReference type="OMA" id="DVCDQVH"/>
    <dbReference type="OrthoDB" id="603at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01896">
        <property type="entry name" value="DRG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000016">
        <property type="entry name" value="Developmentally-regulated GTP-binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000740">
        <property type="entry name" value="Putative GTP-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1070">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045001">
        <property type="entry name" value="DRG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031662">
        <property type="entry name" value="GTP-binding_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006074">
        <property type="entry name" value="GTP1-OBG_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43127">
        <property type="entry name" value="DEVELOPMENTALLY-REGULATED GTP-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16897">
        <property type="entry name" value="MMR_HSR1_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02824">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00905">
        <property type="entry name" value="GTP1_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51880">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OBG-type G" evidence="7">
        <location>
            <begin position="63"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="TGS" evidence="8">
        <location>
            <begin position="288"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00057286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00063574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00072735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00080793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000004913.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="364" mass="40710" checksum="FE00C1ABFA482A33" modified="2011-07-27" version="1">MGILEKISEIEKEIQRTQKNKATEYHLGLLKAKLARYRAQLLEGSKTSGPKGEGFDVMKSGDARVALVGFPSVGKSTLLSTLTSTTSECASYEFTTLTCIPGVIEHNGASIQLLDLPGIIEGAAAGKGRGRQVISVARTADIVVMMLDALKGDIQKELLTKELEAVGIRLNKKRPNIYFKEKKGGGISITATCILTKISERMINLILHEYKIFNADVVFRDDCGTEEFIDVVVGKRKYMPCLYVYNKIDQISIEEVDRIAWQPHCVVISCNMLLNLEHLKEMIWRYLHLITVYTKKPGKKPDFNDGLILRSNCTVEHACHAIHRTLVQNFKYAQVWGHSAKYNGQHVGLSHKLSHEDVLLVTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>F6RKI1</accession>
    <name>F6RKI1_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">wild type</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM76 family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01000987">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6RKI1"/>
    <dbReference type="FunCoup" id="F6RKI1">
        <property type="interactions" value="647"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSCINT00000001184.3">
        <property type="protein sequence ID" value="ENSCINP00000001184.3"/>
        <property type="gene ID" value="ENSCING00000000643.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000169021"/>
    <dbReference type="HOGENOM" id="CLU_029220_1_0_1"/>
    <dbReference type="InParanoid" id="F6RKI1"/>
    <dbReference type="OMA" id="CACAYKR"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032017">
        <property type="entry name" value="FAM76"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46176">
        <property type="entry name" value="LD21662P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46176:SF1">
        <property type="entry name" value="LD21662P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16046">
        <property type="entry name" value="FAM76"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="164"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="164"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="200"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000001184.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="330" mass="38455" checksum="4C0C1A9DD4E21E46" modified="2012-04-18" version="2">MEQKGVQYACTKCMQWFDFDDLSADVQMCKEFLFNNTFPCRYCQTYIKVKVPGEKRICERCFKNRKEYGEPKICMFCTLNSAFIGSKCQRCASSKKKYGMPVNCQKCNLKAAFDHKKAHDCSKQIDGLLLCWKCYVSMQRYQKREKRKGRELLGEDHCLKSGKKSENLKLKSHNEKKHELRKQAQINSRNLNKHHSSSSLDDRIQKMEKRGTPPVSVSSPRRNEEKPGSNTSTPRILGTALPQMNDNIVLVTKLHEEINSLKKNIAKKDQVMLEKDKQISMLKVEHLRLERSLQTKLKTQEEEFFRKMEAMGIKNQELIKKVAQLSKKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>F6S0N6</accession>
    <name>F6S0N6_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Deaminated glutathione amidase</fullName>
            <ecNumber evidence="3">3.5.1.128</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Nitrilase homolog 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">wild type</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the carbon-nitrogen hydrolase superfamily. NIT1/NIT2 family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.128" evidence="3"/>
    <dbReference type="EMBL" id="EAAA01002659">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6S0N6"/>
    <dbReference type="FunCoup" id="F6S0N6">
        <property type="interactions" value="213"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000006878"/>
    <dbReference type="Ensembl" id="ENSCINT00000006878.3">
        <property type="protein sequence ID" value="ENSCINP00000006878.3"/>
        <property type="gene ID" value="ENSCING00000003359.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00550000075099"/>
    <dbReference type="HOGENOM" id="CLU_030130_1_2_1"/>
    <dbReference type="InParanoid" id="F6S0N6"/>
    <dbReference type="OMA" id="MRVAVCQ"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110050">
        <property type="term" value="F:deaminated glutathione amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07572">
        <property type="entry name" value="nit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.110.10:FF:000005">
        <property type="entry name" value="nitrilase homolog 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045254">
        <property type="entry name" value="Nit1/2_C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001110">
        <property type="entry name" value="UPF0012_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23088:SF27">
        <property type="entry name" value="DEAMINATED GLUTATHIONE AMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23088">
        <property type="entry name" value="NITRILASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01227">
        <property type="entry name" value="UPF0012"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CN hydrolase" evidence="6">
        <location>
            <begin position="2"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00066912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00074513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00080297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000006878.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="273" mass="30369" checksum="35CF54B7255A5E87" modified="2012-04-18" version="2">NPIIGVCQLSVGSDIEKNLQQAKDLVKECKELGAVFVFLPEACDYLCEDQKESINMAHTLTSKICIEYCKLAAELSVWISLGGIHRKCEGDPENKIRNSHIVINDVGEVINVYDKCHLFNVDIPNQVKLQKTDFVLPGEHIGKPIETPIGNIGALVCYDIRFPQISTELRKRGAEILTYPSAFTVPTGSAHWEVLLRARAIENQCYVIAAAQTGTHNSTRKSYGHSMVVDPWGTVVACAADKVGAITAKLDLNHLRKLRKEMPVFQHTRNDLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>F6S2J4</accession>
    <name>F6S2J4_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Bile salt export pump</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">wild type</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001523">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="EAAA01001524">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6S2J4"/>
    <dbReference type="STRING" id="7719.ENSCINP00000018558"/>
    <dbReference type="Ensembl" id="ENSCINT00000018558.3">
        <property type="protein sequence ID" value="ENSCINP00000018558.3"/>
        <property type="gene ID" value="ENSCING00000009135.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165732"/>
    <dbReference type="HOGENOM" id="CLU_000604_17_2_1"/>
    <dbReference type="InParanoid" id="F6S2J4"/>
    <dbReference type="OMA" id="MAYFDTY"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042626">
        <property type="term" value="F:ATPase-coupled transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18578">
        <property type="entry name" value="ABC_6TM_Pgp_ABCB1_D2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03249">
        <property type="entry name" value="ABC_MTABC3_MDL1_MDL2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000066">
        <property type="entry name" value="ABC transporter B family member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000121">
        <property type="entry name" value="ABC transporter B family member 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000479">
        <property type="entry name" value="Multidrug resistance protein 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394:SF18">
        <property type="entry name" value="ABC TRANSPORTER B FAMILY MEMBER 11-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394">
        <property type="entry name" value="ATP-DEPENDENT PERMEASE MDL1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="7"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="420"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="463"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="15">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="14">
        <location>
            <begin position="87"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="15">
        <location>
            <begin position="424"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="14">
        <location>
            <begin position="746"/>
            <end position="984"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="324"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="326"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000018558.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="989" mass="107828" checksum="3615F898A5732C04" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
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                <person name="Putnam N."/>
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                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
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                <person name="Meinertzhagen I.A."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
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                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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            <authorList>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
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                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
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                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Putnam N."/>
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                <person name="Saiga H."/>
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                <person name="Terry A."/>
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                <person name="Wang H.G."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="De Tomaso A."/>
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                <person name="Meinertzhagen I.A."/>
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                <person name="Yamada L."/>
                <person name="Wang H.G."/>
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    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000000141.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="341" mass="37094" checksum="B213F281B61B354A" modified="2012-04-18" version="2">MSAVVNTTPVIMTTPAVRPGNKLYAKEFKILGVIQIMLGTLSIITWTYALTLELTTSSRDVFVYISSGIWCGIFFLIAGILATVSSFNPNRCKVVAGMVMAIFAAIFAATMFGIEVAGSIIISSRSYYIYTPYSSYSCTPYYQASYYKSGTYIPYCSSPYRSSRYISSVYIPSKYISCPSSYSYIYNYYSQSQSIFGVRTSLATLHGFMAFFAIAELVVAIVHSVYCCKFSQTSNMAPQPVVQYTTTYPQTNFTGVAQQMPVTSIGQPIGGAYQNTVYPVTSVPYQASIYANQVAPVSSPPMSPVATGMSTASGSQGIQPPPNYSEADKTGKTGDNEFLVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>F6TBK2</accession>
    <name>F6TBK2_CIOIN</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
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    <organism evidence="1 2">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">wild type</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01002577">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6TBK2"/>
    <dbReference type="Ensembl" id="ENSCINT00000010055.3">
        <property type="protein sequence ID" value="ENSCINP00000010055.3"/>
        <property type="gene ID" value="ENSCING00000004869.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000067722"/>
    <dbReference type="HOGENOM" id="CLU_872985_0_0_1"/>
    <dbReference type="InParanoid" id="F6TBK2"/>
    <dbReference type="OMA" id="TINGHEC"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000010055.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="319" mass="35883" checksum="93DA2D048ACCD9D3" modified="2012-04-18" version="2">MSHIDLLRDLNLTVDDELSCSLSLLDQGIKEALGSTSADWLNILSQWKENVLEKETKDSDKALNTNSLHIPDLLSVPEGGNNVTAVIGKENHEKSEAYADSSKHAIVKHTPLKEPQIKQNMLKRKSDGCVTSLACDSKDVLRRWSSETSKKIKLIHEKSEFNPWVKNKWNNTPLKFANKRDNLQQQIPSQNKALNKTFLQRDNFKSDSCTADKLSSSMTPLKSAITKVKTPNGEQFMECTKQEMNHDGDSDEELLRIAELYEKQQKESSPTIENCSSNHLVVKTTGFKSANNKEIQSTSKSLENVQKVISVSNHENCTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F6THZ9</accession>
    <name>F6THZ9_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
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    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="F6THZ9"/>
    <dbReference type="Ensembl" id="ENSCINT00000014902.3">
        <property type="protein sequence ID" value="ENSCINP00000014902.3"/>
        <property type="gene ID" value="ENSCING00000007263.3"/>
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    <dbReference type="HOGENOM" id="CLU_113139_0_0_1"/>
    <dbReference type="InParanoid" id="F6THZ9"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.15.10.10">
        <property type="entry name" value="Bactericidal permeability-increasing protein, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000014902.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="149" mass="17058" checksum="547DF3039C2DDC4F" modified="2012-04-18" version="2">MKFNNIAAEYDLLKTGLTDINETYFGKVFNTSQAKRAYWLLIIAFSVVLFIWVRQGKPTTTEKPFDAPECIAKILNNTDNLQLKNISLDDISGEWKIPFSDVNYALLHLKMYQFAMATTTVQLTKFPNTVIRFGIEHGSFVIDGDYGFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>F6TN85</accession>
    <name>F6TN85_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ryncolin-4</fullName>
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    <gene>
        <name evidence="4" type="primary">LOC100175128</name>
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    <organism evidence="4 5">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
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    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_026695720.1">
        <property type="nucleotide sequence ID" value="XM_026839919.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6TN85"/>
    <dbReference type="STRING" id="7719.ENSCINP00000021040"/>
    <dbReference type="Ensembl" id="ENSCINT00000021040.3">
        <property type="protein sequence ID" value="ENSCINP00000021040.3"/>
        <property type="gene ID" value="ENSCING00000017001.2"/>
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    <dbReference type="GeneID" id="100175128"/>
    <dbReference type="KEGG" id="cin:100175128"/>
    <dbReference type="GeneTree" id="ENSGT00940000164607"/>
    <dbReference type="HOGENOM" id="CLU_038628_1_3_1"/>
    <dbReference type="InParanoid" id="F6TN85"/>
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    <dbReference type="OrthoDB" id="5968928at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
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    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="entry name" value="FReD"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="3.90.215.10:FF:000001">
        <property type="entry name" value="Tenascin isoform 1"/>
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    </dbReference>
    <dbReference type="Gene3D" id="3.90.215.10">
        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050373">
        <property type="entry name" value="Fibrinogen_C-term_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040941">
        <property type="entry name" value="GGGWT_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143:SF424">
        <property type="entry name" value="FIBRINOGEN C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143">
        <property type="entry name" value="FIBRINOGEN/TENASCIN/ANGIOPOEITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00186">
        <property type="entry name" value="FBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5030169459" evidence="2">
        <location>
            <begin position="20"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="3">
        <location>
            <begin position="46"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000021040.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="262" mass="30104" checksum="2BBB0B25A910EB0B" modified="2012-04-18" version="2">MQMALKYLLFLVLVGPALSIPTSQCIKLIAKLEARIAFLENRTCDNAEITPPQNCQDILNLGYTNNGIYKIFSNGETMKVYCDLTTDNGGWIVIQRRQDGLVDFYRNWADYKFGFGEMSSDFWIGLHNMHLLTREQNYELRIDMKDCNDVNKYATYGSFRVGGESALFKLQIEDYNGTAGDGLSVNNGAYFSTKDRDNDKFHLHCATHRTTGWWYRGNCGDANLNGPYSPCEWYEDSVASYYPFQDEFYGLKFIEMKIRPAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>F6TYS5</accession>
    <name>F6TYS5_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptidyl-prolyl cis-trans isomerase</fullName>
            <shortName evidence="5">PPIase</shortName>
            <ecNumber evidence="5">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">wild type</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Identified in the spliceosome C complex. Interacts with SNW1/SKIP. Interacts with CDC40/PRP17; this interaction leads to CDC40 isomerization. Interacts with RBM22.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the cyclophilin-type PPIase family.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="5"/>
    <dbReference type="EMBL" id="EAAA01002580">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6TYS5"/>
    <dbReference type="FunCoup" id="F6TYS5">
        <property type="interactions" value="1031"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000010020"/>
    <dbReference type="Ensembl" id="ENSCINT00000010020.3">
        <property type="protein sequence ID" value="ENSCINP00000010020.3"/>
        <property type="gene ID" value="ENSCING00000004845.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153189"/>
    <dbReference type="HOGENOM" id="CLU_012062_16_3_1"/>
    <dbReference type="InParanoid" id="F6TYS5"/>
    <dbReference type="OMA" id="ELYNDHA"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000008">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024936">
        <property type="entry name" value="Cyclophilin-type_PPIase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044666">
        <property type="entry name" value="Cyclophilin_A-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625:SF4">
        <property type="entry name" value="PEPTIDYLPROLYL ISOMERASE DOMAIN AND WD REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001467">
        <property type="entry name" value="Peptidylpro_ismrse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="6">
        <location>
            <begin position="8"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00062845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000010020.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="159" mass="17519" checksum="21C77BB393A1FC28" modified="2012-04-18" version="2">TWRPSHVIFETSAGTIEIELYWNHAPKTCANFAELCRRGYYNKTIFHRIITNFMIQGGDPTGTGRGGSSIYGGQFEDEFSRELKHTGAGVLSMANSGPNTNGSQFFITLAPTQWLDMKHTIFGRVSGGIKVVNLLGMVETSAQDRPVQDVVVRNAYAAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>F6U182</accession>
    <name>F6U182_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MOB kinase activator-like 4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
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                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
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                <person name="Endo T."/>
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            </authorList>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <property type="term" value="C:Z disc"/>
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        <property type="entry name" value="MOB_kinase_act_fam"/>
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            <begin position="1"/>
            <end position="22"/>
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    <feature type="binding site" evidence="1">
        <location>
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        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
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        <location>
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            <name>Zn(2+)</name>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
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        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    <sequence length="183" mass="20924" checksum="7CBE66BE7F1E9BF4" modified="2012-04-18" version="2">MVSSGSAMDPEQVVRRNPPGTKAEDFYKWSPQSFDEMDSTLAVQQYIQQTIRQDFTDTETILTAPPGQDEGVWKYEQLRQFCLELNGLAIKLQAECTPMTCSQMTATEQWIFLCAAHKTPKECPAIDYTRHTLDGAACLLNSNKYFPSRVSIKDSSVAKLGSVCRRVYRIFSHAYYHHRHIFD</sequence>
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    <accession>F6U1H7</accession>
    <accession>A0A1W2W2E5</accession>
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            <fullName evidence="5">IF rod domain-containing protein</fullName>
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        <name evidence="6" type="primary">LOC100176656</name>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
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    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">wild type</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="NM_001100432.2"/>
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        <property type="protein sequence ID" value="ENSCINP00000001638.3"/>
        <property type="gene ID" value="ENSCING00000000897.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100176656"/>
    <dbReference type="KEGG" id="cin:100176656"/>
    <dbReference type="GeneTree" id="ENSGT00940000168319"/>
    <dbReference type="HOGENOM" id="CLU_012560_9_2_1"/>
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    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 12"/>
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        <property type="term" value="C:intermediate filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="C:nuclear lamina"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="P:heterochromatin formation"/>
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            <begin position="30"/>
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        <location>
            <begin position="1"/>
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        <location>
            <begin position="445"/>
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        <location>
            <begin position="140"/>
            <end position="220"/>
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    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="245"/>
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    </feature>
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            <begin position="401"/>
            <end position="410"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51842"/>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000001638.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="465" mass="53772" checksum="173D2FECE267AF00" modified="2012-04-18" version="2">MATPKQQTRSRDKECPSTPLSPSISSRKDERDQLANLNNRMAAYVEKVRSLETENSRLQIKVTSYEETSTREVTSLKAMYEREISEARRLLDELSKEKAQLQIESNSFREKWEIELNKASALGHDNAVLEGKLKDSNSLLFSRENQLQCAKREREEAVEEAETLRSKLNDIQVELEASQKALDTETLMRVNFENKLQSLKEELEFKEKLYQEEVSEIRKRHTLSVTEIDEKVKGVYESQFEVALIELRDQQNADLEQMKEDLEGQYSSKIEELKKKLDYVRKSTMKGSEDCKAAEMKVERLQTTIKSLQNKNNNLNNRVQDLEGQVATEREIRFSAVLSIEQQRDEALQKITTMEKEYLELMDLKIKLDHEIGVYRKLLEEEETRLNLSPSPHVDGSDSKRRSRRKRKRVTTQSELAQKAKKQEIASATYSSETVITQKSVNVGEDVLHQQDDPAPEGNNSCSIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>F6U3K8</accession>
    <accession>A0A1W2W739</accession>
    <name>F6U3K8_CIOIN</name>
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        <recommendedName>
            <fullName evidence="4">2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100181040</name>
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    <organism evidence="5 6">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the IspD/TarI cytidylyltransferase family. IspD subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002125703.2">
        <property type="nucleotide sequence ID" value="XM_002125667.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6U3K8"/>
    <dbReference type="FunCoup" id="F6U3K8">
        <property type="interactions" value="8"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000023550"/>
    <dbReference type="Ensembl" id="ENSCINT00000023796.2">
        <property type="protein sequence ID" value="ENSCINP00000023550.2"/>
        <property type="gene ID" value="ENSCING00000012680.2"/>
    </dbReference>
    <dbReference type="GeneID" id="100181040"/>
    <dbReference type="KEGG" id="cin:100181040"/>
    <dbReference type="GeneTree" id="ENSGT00390000006412"/>
    <dbReference type="HOGENOM" id="CLU_033636_0_0_1"/>
    <dbReference type="InParanoid" id="F6U3K8"/>
    <dbReference type="OMA" id="RIDCITH"/>
    <dbReference type="OrthoDB" id="414267at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050518">
        <property type="term" value="F:2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047349">
        <property type="term" value="F:D-ribitol-5-phosphate cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035269">
        <property type="term" value="P:protein O-linked glycosylation via mannose"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02516">
        <property type="entry name" value="CDP-ME_synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000003">
        <property type="entry name" value="2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034683">
        <property type="entry name" value="IspD/TarI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018294">
        <property type="entry name" value="ISPD_synthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43015">
        <property type="entry name" value="D-RIBITOL-5-PHOSPHATE CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43015:SF1">
        <property type="entry name" value="D-RIBITOL-5-PHOSPHATE CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01128">
        <property type="entry name" value="IspD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01295">
        <property type="entry name" value="ISPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00069967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000023550.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="401" mass="45531" checksum="438B759F5DAB2EF9" modified="2012-04-18" version="2">MSLTKIEACVVIPASGSGERYNMHPPKQFECLGQKPIISHTISAFERIDCITHIVVTTPQDDDLQLMMESIVKKFHHIKVHLTKGANSRHRSIFEGLKCLKCILKSENIARDPVVIIHDGVRPFVDKNTIMDVIQNTKIYKAIGIVRPLVSTVISVDKDDFLKESLDRSLYWASEMPQGYMFSLIYEAYLKCSDEELNHGTECLKIASDYSAFKPKILKGGDNLWKITYQRDMAVAREFVSSHHLRVYIDENDKTHCCIQDLVSSENLNVLLFAKEELSASNLKSDNCLLFYSSLSLQSFESNLSFLIKSIENYTEDPNNWSDKYGSSNIVAIFCAQTTEMSSLNEVHTMLHRHSHKLLSHNVHLYCIIATQSTLSEPTLRCLNKILGDHEGTFAGQVFLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>F6U8M1</accession>
    <name>F6U8M1_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Homocysteine-responsive endoplasmic reticulum-resident ubiquitin-like domain member 2 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100175173</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">wild type</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="EAAA01001699">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6U8M1"/>
    <dbReference type="FunCoup" id="F6U8M1">
        <property type="interactions" value="428"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000019430"/>
    <dbReference type="Ensembl" id="ENSCINT00000019430.3">
        <property type="protein sequence ID" value="ENSCINP00000019430.3"/>
        <property type="gene ID" value="ENSCING00000009547.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000017671"/>
    <dbReference type="HOGENOM" id="CLU_058243_0_0_1"/>
    <dbReference type="InParanoid" id="F6U8M1"/>
    <dbReference type="OMA" id="YMQLMAA"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030968">
        <property type="term" value="P:endoplasmic reticulum unfolded protein response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000046">
        <property type="entry name" value="Homocysteine-responsive endoplasmic reticulum-resident ubiquitin-like domain member 2 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039751">
        <property type="entry name" value="HERPUD1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12943:SF27">
        <property type="entry name" value="HOMOCYSTEINE-INDUCED ENDOPLASMIC RETICULUM PROTEIN, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12943">
        <property type="entry name" value="HOMOCYSTEINE-RESPONSIVE ENDOPLASMIC RETICULUM-RESIDENT UNIQUITIN-LIKE DOMAIN HERPUD PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0834">Unfolded protein response</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="260"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="8">
        <location>
            <begin position="6"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="85"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="284"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="85"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="113"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="299"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000019430.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="343" mass="38911" checksum="8CB46FEF5DFDA8B2" modified="2012-04-18" version="2">MSGTKIRISIRAANQHQADQVFDCEKQWQVKKLKELIKESYAGKPKPEEQRLIYGGKYLLDHEVLGSVLKVSNLPNIVHLVTSISSNPDSIQPTQTSTNDTSTLPTELRQRHTASNSQPNPMTHWQQMYSQPYMQQFTSTQMTPEQYNQAWQQYQQQMAEYMQYYGMQVPGMHMPFTASVTQTTTPAAAVAHPEPNIAPNAPEPVRPAPDNNPPMNAQGGFAAADDDDPMRRDWLDVFFTMLRGCFFLSIVYFYSSFWRFIFILSLFVIIYIYQSGFFQVQRRHPVPQQPDNNNNGEMSTDDNDELPSEPDVPPPPSLSATAWCFVSTFFTSLIPQGPPVGAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F6UC10</accession>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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        <property type="entry name" value="LEUCINE-RICH REPEAT AND IQ DOMAIN-CONTAINING PROTEIN 1-RELATED"/>
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        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 9"/>
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        <property type="match status" value="2"/>
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        <property type="entry name" value="LRR_TYP"/>
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        <property type="entry name" value="L domain-like"/>
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        <property type="entry name" value="Outer arm dynein light chain 1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="LRR"/>
        <property type="match status" value="9"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
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    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="645"/>
            <end position="672"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000027144.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
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    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1374" mass="156455" checksum="41B307508763BFFF" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>F6UM41</accession>
    <name>F6UM41_CIOIN</name>
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        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
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            <taxon>Metazoa</taxon>
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            <taxon>Tunicata</taxon>
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            <taxon>Phlebobranchia</taxon>
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    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="F6UM41"/>
    <dbReference type="STRING" id="7719.ENSCINP00000003196"/>
    <dbReference type="Ensembl" id="ENSCINT00000003196.2">
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        <property type="gene ID" value="ENSCING00000001596.2"/>
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    <dbReference type="HOGENOM" id="CLU_1299346_0_0_1"/>
    <dbReference type="InParanoid" id="F6UM41"/>
    <dbReference type="OMA" id="TWAENIS"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050930">
        <property type="entry name" value="MFS_Vesicular_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23506">
        <property type="entry name" value="GH10249P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23506:SF26">
        <property type="entry name" value="MFS-TYPE TRANSPORTER SLC18B1"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="MFS general substrate transporter"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
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    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
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            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="60"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="160"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000003196.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="212" mass="22966" checksum="7BFAFC3C8A9CDE63" modified="2012-04-18" version="2">MFTLSSFFASNLDTFLNDEFDMSPVIVGVTMLIGSLCYGITSPIWGYIADRWKKNFLMEIGTLGEFVAMVLIGPALLLKSWFGIEKQTLPLLYAAICVKGSFTGAALMPTFTDMVDAASELKVQESLLAQYGMVSGLWNTAFSLGDISGPLLGGLLVGKFGFENTAAILGLCCLGLMVIKISERIIRRCCCKPTIVESNSEYESLLPNAPDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>F6V1E1</accession>
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        <submittedName>
            <fullName evidence="3">Uncharacterized LOC100178958</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100178958</name>
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    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
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        <lineage>
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            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01001917">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002119358.1">
        <property type="nucleotide sequence ID" value="XM_002119322.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6V1E1"/>
    <dbReference type="Ensembl" id="ENSCINT00000025171.2">
        <property type="protein sequence ID" value="ENSCINP00000024925.2"/>
        <property type="gene ID" value="ENSCING00000013618.2"/>
    </dbReference>
    <dbReference type="GeneID" id="100178958"/>
    <dbReference type="KEGG" id="cin:100178958"/>
    <dbReference type="HOGENOM" id="CLU_1325976_0_0_1"/>
    <dbReference type="InParanoid" id="F6V1E1"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014090249" evidence="2">
        <location>
            <begin position="23"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="159"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="159"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000024925.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="207" mass="23369" checksum="9B4299F3DAE8E43C" modified="2012-04-18" version="2">MKITETMIAFVVIFCVTSSCFGKSISKVSSNDLNEKANGHSIVKRQSNADLNLQNKKTRHMNELIRQYYISSILNEIKGKRDQRQPLTADEKATACRHAVQYCRVGQVEEDNGATYLVDQKRDIENGYPDMDNTEQNNSVPNEPCLSVSAIAKLLTNSRGNSANQRTIDTSQNGHTDGETFDMYTNGQKDLASHLFQFFQDQYDLTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F6V8C7</accession>
    <name>F6V8C7_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01002217">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6V8C7"/>
    <dbReference type="Ensembl" id="ENSCINT00000023065.2">
        <property type="protein sequence ID" value="ENSCINP00000022819.2"/>
        <property type="gene ID" value="ENSCING00000012122.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00580000082143"/>
    <dbReference type="HOGENOM" id="CLU_1079788_0_0_1"/>
    <dbReference type="InParanoid" id="F6V8C7"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="95"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="131"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="172"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="231"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000022819.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="258" mass="29224" checksum="1F92EA9E453E76AF" modified="2012-04-18" version="2">MNATKFEITPTTTVLSKLNHTEYQEWNLLGQKVWTSESKATWSPNKDVAWYTCEAIHITCIVVAFYILYALVHFRILRKAEVAKTSARLRKGGRWLENLCIMGVLTALLRFGNNQALLFTVDSTSRCSTLLNVSIALYNVTIHPIYTFLWVRLRIFYSNRALKHLYSKTVRFLSWVALLCFICVTMVCMVLLIVDRDNSASGRVCANTNSNDSSTVPANSSRPPHQQGVRVNIMTGLLGGCIQLSFLALFLYPIMDNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>F6V9F0</accession>
    <name>F6V9F0_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Serine palmitoyltransferase 1</fullName>
            <ecNumber evidence="5">2.3.1.50</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Long chain base biosynthesis protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Serine-palmitoyl-CoA transferase 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">wild type</strain>
        </source>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Component of the serine palmitoyltransferase multisubunit enzyme (SPT) that catalyzes the initial and rate-limiting step in sphingolipid biosynthesis by condensing L-serine and activated acyl-CoA (most commonly palmitoyl-CoA) to form long-chain bases. The SPT complex is also composed of SPTLC2 or SPTLC3 and SPTSSA or SPTSSB. Within this complex, the heterodimer with SPTLC2 or SPTLC3 forms the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC2-SPTSSA complex shows a strong preference for C16-CoA substrate, while the SPTLC1-SPTLC3-SPTSSA isozyme uses both C14-CoA and C16-CoA as substrates, with a slight preference for C14-CoA. The SPTLC1-SPTLC2-SPTSSB complex shows a strong preference for C18-CoA substrate, while the SPTLC1-SPTLC3-SPTSSB isozyme displays an ability to use a broader range of acyl-CoAs, without apparent preference. Required for adipocyte cell viability and metabolic homeostasis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-serine + hexadecanoyl-CoA + H(+) = 3-oxosphinganine + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:14761"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:58299"/>
            <dbReference type="EC" id="2.3.1.50"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:14762"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>dodecanoyl-CoA + L-serine + H(+) = 3-oxotetradecasphinganine + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:35679"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57375"/>
            <dbReference type="ChEBI" id="CHEBI:71008"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:35680"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>octadecanoyl-CoA + L-serine + H(+) = 3-oxoeicosasphinganine + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:33683"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57394"/>
            <dbReference type="ChEBI" id="CHEBI:65073"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:33684"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>tetradecanoyl-CoA + L-serine + H(+) = 3-oxohexadecasphinganine + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:35675"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57385"/>
            <dbReference type="ChEBI" id="CHEBI:71007"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:35676"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; sphingolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Sphingolipid metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.50" evidence="5"/>
    <dbReference type="EMBL" id="EAAA01002105">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6V9F0"/>
    <dbReference type="FunCoup" id="F6V9F0">
        <property type="interactions" value="40"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000008506"/>
    <dbReference type="Ensembl" id="ENSCINT00000008506.3">
        <property type="protein sequence ID" value="ENSCINP00000008506.3"/>
        <property type="gene ID" value="ENSCING00000012271.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00550000074872"/>
    <dbReference type="HOGENOM" id="CLU_015846_0_0_1"/>
    <dbReference type="InParanoid" id="F6V9F0"/>
    <dbReference type="OMA" id="LTKYGCG"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004758">
        <property type="term" value="F:serine C-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046513">
        <property type="term" value="P:ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>F6VBD5</accession>
    <name>F6VBD5_CIOIN</name>
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            <fullName evidence="9">MRH domain-containing protein</fullName>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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            <taxon>Tunicata</taxon>
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            <taxon>Phlebobranchia</taxon>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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        <property type="entry name" value="CATION-INDEPENDENT MANNOSE-6-PHOSPHATE RECEPTOR"/>
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</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>F6VBZ6</accession>
    <name>F6VBZ6_CIOIN</name>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Telomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the telombin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="F6VBZ6"/>
    <dbReference type="STRING" id="7719.ENSCINP00000027127"/>
    <dbReference type="Ensembl" id="ENSCINT00000027373.2">
        <property type="protein sequence ID" value="ENSCINP00000027127.2"/>
        <property type="gene ID" value="ENSCING00000001453.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000018285"/>
    <dbReference type="HOGENOM" id="CLU_517371_0_0_1"/>
    <dbReference type="InParanoid" id="F6VBZ6"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000783">
        <property type="term" value="C:nuclear telomere cap complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0098505">
        <property type="term" value="F:G-rich strand telomeric DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010521">
        <property type="term" value="F:telomerase inhibitor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032210">
        <property type="term" value="P:regulation of telomere maintenance via telomerase"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016233">
        <property type="term" value="P:telomere capping"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04497">
        <property type="entry name" value="hPOT1_OB1_like"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028389">
        <property type="entry name" value="POT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032042">
        <property type="entry name" value="POT1PC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011564">
        <property type="entry name" value="Telomer_end-bd_POT1/Cdc13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14513">
        <property type="entry name" value="PROTECTION OF TELOMERES 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14513:SF0">
        <property type="entry name" value="PROTECTION OF TELOMERES PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02765">
        <property type="entry name" value="POT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16686">
        <property type="entry name" value="POT1PC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00976">
        <property type="entry name" value="Telo_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0779">Telomere</keyword>
    <feature type="domain" description="Telomeric single stranded DNA binding POT1/Cdc13" evidence="10">
        <location>
            <begin position="11"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="303"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="353"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="492"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="303"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="315"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="364"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="401"/>
            <end position="410"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="418"/>
            <end position="429"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="441"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="495"/>
            <end position="504"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000027127.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="527" mass="58230" checksum="B8CC08979B734DDD" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>F6VDJ9</accession>
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        <recommendedName>
            <fullName evidence="10">Fatty acyl-CoA reductase</fullName>
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        </recommendedName>
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    <organism evidence="13 14">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">wild type</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the reduction of fatty acyl-CoA to fatty alcohols.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>18-methylnonadecanoyl-CoA + 2 NADPH + 2 H(+) = 18-methylnonadecan-1-ol + 2 NADP(+) + CoA</text>
            <dbReference type="Rhea" id="RHEA:81767"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:84914"/>
            <dbReference type="ChEBI" id="CHEBI:231999"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:81768"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a long-chain fatty acyl-CoA + 2 NADPH + 2 H(+) = a long-chain primary fatty alcohol + 2 NADP(+) + CoA</text>
            <dbReference type="Rhea" id="RHEA:52716"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:77396"/>
            <dbReference type="ChEBI" id="CHEBI:83139"/>
            <dbReference type="EC" id="1.2.1.84"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:52717"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>eicosanoyl-CoA + 2 NADPH + 2 H(+) = eicosan-1-ol + 2 NADP(+) + CoA</text>
            <dbReference type="Rhea" id="RHEA:81727"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57380"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:75627"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:81728"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>hexadecanoyl-CoA + 2 NADPH + 2 H(+) = hexadecan-1-ol + 2 NADP(+) + CoA</text>
            <dbReference type="Rhea" id="RHEA:36315"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16125"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.2.1.84"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:36316"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>octadecanoyl-CoA + 2 NADPH + 2 H(+) = octadecan-1-ol + 2 NADP(+) + CoA</text>
            <dbReference type="Rhea" id="RHEA:36319"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:32154"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57394"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.2.1.84"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:36320"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the fatty acyl-CoA reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.84" evidence="10"/>
    <dbReference type="EMBL" id="EAAA01000930">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6VDJ9"/>
    <dbReference type="STRING" id="7719.ENSCINP00000025913"/>
    <dbReference type="Ensembl" id="ENSCINT00000026159.2">
        <property type="protein sequence ID" value="ENSCINP00000025913.2"/>
        <property type="gene ID" value="ENSCING00000014293.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000006367"/>
    <dbReference type="HOGENOM" id="CLU_024661_0_2_1"/>
    <dbReference type="InParanoid" id="F6VDJ9"/>
    <dbReference type="OMA" id="FSECYNE"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102965">
        <property type="term" value="F:alcohol-forming long-chain fatty acyl-CoA reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080019">
        <property type="term" value="F:alcohol-forming very long-chain fatty acyl-CoA reductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035336">
        <property type="term" value="P:long-chain fatty-acyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd05236">
        <property type="entry name" value="FAR-N_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09071">
        <property type="entry name" value="FAR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000639">
        <property type="entry name" value="Fatty acyl-CoA reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026055">
        <property type="entry name" value="FAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033640">
        <property type="entry name" value="FAR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013120">
        <property type="entry name" value="FAR_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11011:SF45">
        <property type="entry name" value="FATTY ACYL-COA REDUCTASE CG8306-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11011">
        <property type="entry name" value="MALE STERILITY PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07993">
        <property type="entry name" value="NAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03015">
        <property type="entry name" value="Sterile"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="4 10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0521">NADP</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="363"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="481"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="domain" description="Thioester reductase (TE)" evidence="12">
        <location>
            <begin position="28"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acyl-CoA reductase C-terminal" evidence="11">
        <location>
            <begin position="373"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF03015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF07993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000025913.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="507" mass="57962" checksum="D29014AC1B0097D8" modified="2012-04-18" version="2">MLPSQPEAPADRTCPTIAEFYAGKTVALTGGTGFLGQGVIEKLLRCCPEIKKIILFIRHKRNVEPKDRLSSLVELPAFDNLWQLQPNFVEKLSFVSCDLEADDLGLSKEDRKTLQNEVNVFYHSAATLKFNEQLRLSFEVNVQCVRRLLKLCKGMHHLHAFVHVSTAYSHCNRYTIDEKVYDTSLDYHDLENSFRWMNDSMVEKITPDVLGNRPNTYTLTKALAEDAVCRESGNLPICIVRPSMIIPAWQEPMPGWCTNLYGPTAFFVAYGKGVLRSVIADKKIVADLIPVDLVVNGVIAAALNDIEAMVSIKHLVPIYNLTTGCHNPLYISDLVSPIKKKWYSTYPLDPLRMPSITVTCNKYLHNASIIMLQTIPAYIYDMVLAVCSKRRKMVKLNSKLNAGMSVMEYFFTNEWRWKQDNTTKLQKSLTPKDQKNFNFDARCFSWSEQIKHYVIGTRQFLVKENMNEYPKARKAVDRLRMMARCVNIVAAIGIYGYCWFVTNLCFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>F6VXD9</accession>
    <name>F6VXD9_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">wild type</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="EAAA01000984">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6VXD9"/>
    <dbReference type="STRING" id="7719.ENSCINP00000015667"/>
    <dbReference type="Ensembl" id="ENSCINT00000015667.3">
        <property type="protein sequence ID" value="ENSCINP00000015667.3"/>
        <property type="gene ID" value="ENSCING00000007645.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153818"/>
    <dbReference type="HOGENOM" id="CLU_035503_0_0_1"/>
    <dbReference type="InParanoid" id="F6VXD9"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030073">
        <property type="term" value="P:insulin secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039066">
        <property type="entry name" value="HNF-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006899">
        <property type="entry name" value="HNF-1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044869">
        <property type="entry name" value="HNF-1_POU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11568">
        <property type="entry name" value="HEPATOCYTE NUCLEAR FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11568:SF1">
        <property type="entry name" value="HEPATOCYTE NUCLEAR FACTOR 1-BETA-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04814">
        <property type="entry name" value="HNF-1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51936">
        <property type="entry name" value="POU_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0371">Homeobox</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="POU-specific atypical" evidence="4">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="3">
        <location>
            <begin position="75"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="1">
        <location>
            <begin position="77"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="164"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="15"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000015667.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="454" mass="50302" checksum="4E4D4E8B6140B4B2" modified="2011-07-27" version="1">MARHNIPQREVVDSTGLNQSHLSQHLNKGTPMKNQKRGLLYAWWTKKQDEVAAQFKIARSGMGAEQVEEAAGVSPRARRNRFKWGPASQRILYEAYQHQRNPTKEEREELVKKCNRAECNQRGVSPSHANGLGSNLVTEVRVYNWFANRRKEDAFRHKLAQDSYLGDQDPCQPSEGSPEHNGPPSKVQIMEQFPKAEPGLYGEQRDHEINRDLIKVEAHDRSHVPTPHATQNISLVTPSEASDSAQRIAQTSLPVVRSSRINQGCRSRAPPGVVITNLPLPDDLQRSDNFSSVSHFGALPSVNTLSPIMNSVFQVQQPSSSQEMDPAQMQSTLPPHSLLLSGIIASVSQAPTGQHMPVFTPLTPGHVVHEHHPLAVTQLHNHSAGGYGPPPTYHSNIKQDFSGYQQGTLAYTLTEQRRDPSISVQDVNSSQSTSLVLSNATVDTPTIVYQQPVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>F6W5F4</accession>
    <name>F6W5F4_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glycoside hydrolase family 31 TIM barrel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">wild type</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the glycosyl hydrolase 31 family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002402">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6W5F4"/>
    <dbReference type="STRING" id="7719.ENSCINP00000016087"/>
    <dbReference type="Ensembl" id="ENSCINT00000016087.3">
        <property type="protein sequence ID" value="ENSCINP00000016087.3"/>
        <property type="gene ID" value="ENSCING00000007859.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164162"/>
    <dbReference type="HOGENOM" id="CLU_000631_1_0_1"/>
    <dbReference type="InParanoid" id="F6W5F4"/>
    <dbReference type="OMA" id="WWHTEIA"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000322">
        <property type="entry name" value="Glyco_hydro_31_TIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22762">
        <property type="entry name" value="ALPHA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22762:SF133">
        <property type="entry name" value="P-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01055">
        <property type="entry name" value="Glyco_hydro_31_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycoside hydrolase family 31 TIM barrel" evidence="3">
        <location>
            <begin position="1"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU361185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000016087.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="199" mass="23306" checksum="81F133EE8374DDE0" modified="2012-04-18" version="2">MRDARIPFDIQYGDIDYMDAKKDFTYDPVKYAGLPAYVDQLHDWGMRYVIILDPGIKIEPGYKAYDEGMQQDIFMKNPDGTSPVLTEVWPGDTYHPDFTHSAASQWWTDQCRDFHDNQGVHFDALWIDMNEPANFQTDDPTKRELMNCTGIYNFPPYLPRILGYWVGMYDKTFCMDNIQEWGLHYNVHSLYGHTMSQAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>F6WAD4</accession>
    <name>F6WAD4_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Vesicle-fusing ATPase</fullName>
            <ecNumber evidence="7">3.6.4.6</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">wild type</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 7">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="7">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.6" evidence="7"/>
    <dbReference type="EMBL" id="EAAA01002949">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6WAD4"/>
    <dbReference type="FunCoup" id="F6WAD4">
        <property type="interactions" value="487"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000014222"/>
    <dbReference type="Ensembl" id="ENSCINT00000014222.3">
        <property type="protein sequence ID" value="ENSCINP00000014222.3"/>
        <property type="gene ID" value="ENSCING00000006914.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000064085"/>
    <dbReference type="HOGENOM" id="CLU_008037_2_1_1"/>
    <dbReference type="InParanoid" id="F6WAD4"/>
    <dbReference type="OMA" id="DGMHALN"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035494">
        <property type="term" value="P:SNARE complex disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000154">
        <property type="entry name" value="Vesicle-fusing ATPase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000026">
        <property type="entry name" value="vesicle-fusing ATPase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000166">
        <property type="entry name" value="vesicle-fusing ATPase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054419">
        <property type="entry name" value="NSF_ATPase_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039812">
        <property type="entry name" value="Vesicle-fus_ATPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23078:SF3">
        <property type="entry name" value="VESICLE-FUSING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23078">
        <property type="entry name" value="VESICULAR-FUSION PROTEIN NSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21964">
        <property type="entry name" value="NSF_ATPase_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <location>
            <begin position="252"/>
            <end position="387"/>
        </location>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU367045"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000014222.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="461" mass="50665" checksum="321643AB6CB06B04" modified="2012-04-18" version="2">IVNGPEILNKYVGESESNIRKLFEAAEEEQKKLGINSGLHIIIFDEIDAICKQRGSVSGASGVHDTVVNQLLSKIDGVEPLNNILVIGMTNRPDLIDDALLRPGRLEVKKEIGLPDENGRLEILNIHTEKMKSSDMLDDDVNLREIAQLTKNFSGAELAGLVGAAQSCAFVRHTKAGSKVEVDLDTIDELKVCRADFMAGLENDIKPAYGAKEEDIERFMRNGVLMWGRPVQTALDSGDLLVNQVRNSNKTPLVSVLITGAVGCGKTALATRIALDSGFPFLKICSPGELVGFSEAAKCQHLKKVFDDAYKSQLSCVVIDDIEKLIDYNPIGPRFSNTVTQALMVLFKNLPPPKRRLLVIGTSSNKDLLHEMGMEKCFSSHIHVPCLSKAQHITTTLELLDAFTPEERCSIERLISGRSAWVGIKSLIHMVGTAEQAESTMRVPTFLTLLEEEAHNPLIFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>F6WIL0</accession>
    <name>F6WIL0_CIOIN</name>
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        <recommendedName>
            <fullName evidence="3">ZP domain-containing protein</fullName>
        </recommendedName>
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    <organism evidence="4 5">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
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            <taxon>Ciona</taxon>
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    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">wild type</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01002394">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6WIL0"/>
    <dbReference type="STRING" id="7719.ENSCINP00000015655"/>
    <dbReference type="Ensembl" id="ENSCINT00000015655.3">
        <property type="protein sequence ID" value="ENSCINP00000015655.3"/>
        <property type="gene ID" value="ENSCING00000007638.3"/>
    </dbReference>
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    <dbReference type="HOGENOM" id="CLU_840792_0_0_1"/>
    <dbReference type="InParanoid" id="F6WIL0"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.4100">
        <property type="entry name" value="Zona pellucida, ZP-C domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.3210">
        <property type="entry name" value="Zona pellucida, ZP-N domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055355">
        <property type="entry name" value="ZP-C"/>
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        <property type="entry name" value="ZP-C_dom"/>
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        <property type="entry name" value="ZP-N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001507">
        <property type="entry name" value="ZP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11576:SF22">
        <property type="entry name" value="ONCOPROTEIN INDUCED TRANSCRIPT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11576">
        <property type="entry name" value="ZONA PELLUCIDA SPERM-BINDING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00100">
        <property type="entry name" value="Zona_pellucida"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23344">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51034">
        <property type="entry name" value="ZP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ZP" evidence="3">
        <location>
            <begin position="1"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="290"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000015655.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="331" mass="36589" checksum="E541B24EA2D96680" modified="2012-04-18" version="2">MDITVSACSVDNNALSLGTSAPGTQPIPEECRVKSEEDSHKFSFGLSDCSMRVEEDFWTIVYSNTVRLFADDSKDDETQNSNSPITRGAWFYVPVSCTFQKHGNLTTSFQPVEREVDLLSVPGMGSFSFFLDFYNSSDFSVAIGPYDYPLQFTVNSDIYFGLRAVGGGTDMQLFTVNCIAYPTADINDNTNSYTMIGNGCILDSTMVKHPAASSMENHFSIKAFQFRTIANNTGPIVVHIQCSVIVCVSNNSITRCSQGCVSDDQATRRRREIIRSSFAVNHRYKRQLIEDDDDSIPNNNDPNVTQSPVQQVNSSGDIQIIIEVNSNPSPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>F6WQS5</accession>
    <accession>A0A1W2WDV7</accession>
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        <recommendedName>
            <fullName evidence="4 5">Endoplasmic reticulum junction formation protein lunapark</fullName>
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    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100180661</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">wild type</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Plays a role in determining ER morphology.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer; homodimerization requires the C4-type zinc finger motif and decreases during mitosis in a phosphorylation-dependent manner.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="5">The C4-type zinc finger motif is necessary both for its ER three-way tubular junction localization and formation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the lunapark family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01000296">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002127399.1">
        <property type="nucleotide sequence ID" value="XM_002127363.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6WQS5"/>
    <dbReference type="FunCoup" id="F6WQS5">
        <property type="interactions" value="752"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000009459"/>
    <dbReference type="Ensembl" id="ENSCINT00000009459.3">
        <property type="protein sequence ID" value="ENSCINP00000009459.3"/>
        <property type="gene ID" value="ENSCING00000004582.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100180661"/>
    <dbReference type="KEGG" id="cin:100180661"/>
    <dbReference type="GeneTree" id="ENSGT00390000001859"/>
    <dbReference type="HOGENOM" id="CLU_036951_0_0_1"/>
    <dbReference type="InParanoid" id="F6WQS5"/>
    <dbReference type="OMA" id="CGYFNPS"/>
    <dbReference type="OrthoDB" id="1725934at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071782">
        <property type="term" value="C:endoplasmic reticulum tubular network"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098826">
        <property type="term" value="C:endoplasmic reticulum tubular network membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071788">
        <property type="term" value="P:endoplasmic reticulum tubular network maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071786">
        <property type="term" value="P:endoplasmic reticulum tubular network organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903373">
        <property type="term" value="P:positive regulation of endoplasmic reticulum tubular network organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040115">
        <property type="entry name" value="Lnp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019273">
        <property type="entry name" value="Lunapark_Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22166">
        <property type="entry name" value="ENDOPLASMIC RETICULUM JUNCTION FORMATION PROTEIN LUNAPARK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22166:SF12">
        <property type="entry name" value="ENDOPLASMIC RETICULUM JUNCTION FORMATION PROTEIN LUNAPARK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10058">
        <property type="entry name" value="Zn_ribbon_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="42"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="77"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="Lunapark zinc ribbon" evidence="8">
        <location>
            <begin position="232"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="141"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="285"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="102"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="141"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="350"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00047002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00049772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU367073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF10058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000009459.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="383" mass="43771" checksum="A016AD157F1399C3" modified="2011-07-27" version="1">MGSLLSRWKKSKTTTEVLEDIDKSISQLEKFRKSNQELRSKVIGNLILYSVLLYIVACVSLYFLITPTTWQHRCLQLLPLLCFPFIIYFLKRFLHWWFVRKISRNELELQALRDERKEILENVMETETYKKAKEILERFDPETKKKLEEERQRRENPTPSPTTPGTELRRRGAPMPTPAAPNTPGFSGTPATTPMRGPVNSNMTPMNGNMRGSLPPVATLPRPLIQQNRSSMEKIVEYFVGDGPSNRYALICRNCYSHNGMALKEEFEYIEYRCAYCRFFNPSRKQRPNAPRLPSHVTPKQNPGTPKPMDRDSGNDMSPPPSSKSSENGDAAVLPDPVESPVNVSENTEEPTKLELETKSEDKAEEPLIEEKAPSLKEIPATE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>F6WY52</accession>
    <name>F6WY52_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">wild type</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2"/>
    <dbReference type="EMBL" id="EAAA01002308">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6WY52"/>
    <dbReference type="STRING" id="7719.ENSCINP00000021348"/>
    <dbReference type="Ensembl" id="ENSCINT00000021348.3">
        <property type="protein sequence ID" value="ENSCINP00000021348.3"/>
        <property type="gene ID" value="ENSCING00000010806.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000169786"/>
    <dbReference type="HOGENOM" id="CLU_001645_8_0_1"/>
    <dbReference type="InParanoid" id="F6WY52"/>
    <dbReference type="OMA" id="DYNCENI"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00047">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000185">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000145">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase epsilon-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050348">
        <property type="entry name" value="Protein-Tyr_Phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134">
        <property type="entry name" value="RECEPTOR-TYPE TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134:SF527">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE NON-RECEPTOR TYPE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="5">
        <location>
            <begin position="1"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="6">
        <location>
            <begin position="89"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="5">
        <location>
            <begin position="236"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="6">
        <location>
            <begin position="386"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009580"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000021348.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="485" mass="55043" checksum="42409C256E6814A4" modified="2012-04-18" version="2">QDEVAVVVMLTNVTEGGKRKCEKYWPEPNETLTFGPFEVKAKEEGYYGCYVVRTFEVANKDNVYESVIRKLKHFQYVTWPDHGVPVSTTGFYRLHTHVMEEHNSAGNEAPIVVHCSAGVGRTGTFIGFDCLVRMIEDKAAANVFEVVLNMRMQRTDMVQTKAQYALLYKLITETYVLKNTDFAGEELDEKIANLTRTNRSTHKSGFEVEFSNTNIIEPLTTTKDIVVYNVIQSLFIFKYKVNSKRKTCRLVLLPPMQGDTDLTYYNASTILGYGKSETAIAAQGPIRHDMVEKFWLAITSLHVRTIVMLNNFFENDTVQCHDYFPTCVGDSKQYGDTTVRLASCDTSDNITSRKLQVIKDKIQAKMEINHYQLSGWPDKGVPRTCESVLDLAAKTQTSSQQQGGGVLVHCRDGSGRTGTFLAIINVIERIKNENQIDVFRTVKDLRDSRPEMVSNLQQYKFIYNTASAYMRSFATYDNFTPAVKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>F6X0L7</accession>
    <name>F6X0L7_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">PNK FHA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="entry name" value="P-loop_NTPase"/>
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    <dbReference type="InterPro" id="IPR013954">
        <property type="entry name" value="PNK3P"/>
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        <property type="entry name" value="Polynucleotide_phosphatase"/>
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        <property type="entry name" value="HAD-SF-IIIA"/>
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        <property type="entry name" value="BIFUNCTIONAL POLYNUCLEOTIDE PHOSPHATASE_KINASE"/>
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    <dbReference type="Pfam" id="PF13671">
        <property type="entry name" value="AAA_33"/>
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        <property type="entry name" value="FHA_2"/>
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        <property type="entry name" value="HAD-like"/>
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        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
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    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
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        <location>
            <begin position="4"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="172"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="Pfam" id="PF17913"/>
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    <evidence type="ECO:0000313" key="8">
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    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
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    </evidence>
    <sequence length="571" mass="64583" checksum="6F61D40D58D26987" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>F6XYE9</accession>
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        <recommendedName>
            <fullName evidence="11">Caspase family p20 domain-containing protein</fullName>
        </recommendedName>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">wild type</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the peptidase C14A family.</text>
    </comment>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="F6XYE9"/>
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        <property type="protein sequence ID" value="ENSCINP00000015083.3"/>
        <property type="gene ID" value="ENSCING00000007344.3"/>
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        <property type="component" value="Chromosome 8"/>
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    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
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        <property type="project" value="GO_Central"/>
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        <property type="term" value="F:cysteine-type endopeptidase activity"/>
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        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0043525">
        <property type="term" value="P:positive regulation of neuron apoptotic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="CDD" id="cd00032">
        <property type="entry name" value="CASc"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Caspase, apoptotic cysteine protease, putative"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="3.30.70.1470">
        <property type="entry name" value="Caspase-like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Caspase-like_dom_sf"/>
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        <property type="entry name" value="Caspase_his_AS"/>
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        <property type="entry name" value="AT03047P-RELATED"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="IL1BCENZYME"/>
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    <dbReference type="SMART" id="SM00115">
        <property type="entry name" value="CASc"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF52129">
        <property type="entry name" value="Caspase-like"/>
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        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS01121">
        <property type="entry name" value="CASPASE_HIS"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="CASPASE_P10"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS50208">
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="5" id="KW-0865">Zymogen</keyword>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="PROSITE" id="PS50208"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
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    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
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    <accession>F6Y5W9</accession>
    <name>F6Y5W9_CIOIN</name>
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        <recommendedName>
            <fullName evidence="2">SAM domain-containing protein</fullName>
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    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </evidence>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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                <consortium name="Ensembl"/>
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        <property type="entry name" value="DNAJ_1"/>
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        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="2">
        <location>
            <begin position="4"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000012873.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="342" mass="38567" checksum="BB1C13271E7CEDA4" modified="2012-04-18" version="2">MGKDYYKVLGISKSASEDEIKKAYRKLALKYHPDKNKSASAEEKFKEIAEAYEVLSDPEKKKMYDTHGEQGLNGGMSKDGDSYSYSFHGDPKATFEAFFGTSNPFASFFGGQNDVEDMMFENSDGSFGQGGSFFQSNFSRGSPRHRADNVSCQFSQRGQPTQDPPIHCDLKCSLEDIYKGGSRKMKITRKRLNPDGYSTRNEDKILNIDIKKGWKEGTKITFPKEGDEKPNTIPADIVFTLKDTEHDKFKRDGSNIIYTDTVTLKQALTGFTAMIPTLDNGRNIPLPCTDIIKPDTQKRIRGEGLPLPKQPHRRGDLLVNFNIVFPDYLTRQNKNVLKDVLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F6YHY2</accession>
    <name>F6YHY2_CIOIN</name>
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        <submittedName>
            <fullName evidence="3">P17/29C-like protein DDB_G0287399</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100185902</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
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    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6YHY2"/>
    <dbReference type="Ensembl" id="ENSCINT00000022968.2">
        <property type="protein sequence ID" value="ENSCINP00000022722.2"/>
        <property type="gene ID" value="ENSCING00000012065.2"/>
    </dbReference>
    <dbReference type="GeneID" id="100185902"/>
    <dbReference type="HOGENOM" id="CLU_1474672_0_0_1"/>
    <dbReference type="InParanoid" id="F6YHY2"/>
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        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5030169702" evidence="2">
        <location>
            <begin position="21"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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    </feature>
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        <location>
            <begin position="129"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="160"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="171"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000022722.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="183" mass="18771" checksum="CCF5ED7647DF4433" modified="2012-04-18" version="2">MTRTIKLLLILVCLFGYIEGKSLARPIMRHEISVNSRTTVHLLKRIRRSRAPEIGSAGLPSGHAGRPSKPDREGSVSGCDGTTDDEDCISRTTGSSGCRNRDQEGSASGWDGITDDEDCVVGSAVEPFSGSASGSGDEGESSASSGDCYGTTSDDEDCIPGGSTSGPIRPGSGGGSQEIGSGK</sequence>
</entry>
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        <recommendedName>
            <fullName evidence="2">MIT domain-containing protein</fullName>
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        <name type="scientific">Ciona intestinalis</name>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
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                <person name="Chapman J."/>
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                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
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                <person name="Francino P."/>
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                <person name="Hayashi H."/>
                <person name="Hino K."/>
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                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
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                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
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                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
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                <person name="Sasaki A."/>
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                <person name="Shoguchi E."/>
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                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
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            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
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            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
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    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="ENSCINP00000015038.3"/>
        <property type="gene ID" value="ENSCING00000007325.3"/>
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    <dbReference type="GeneTree" id="ENSGT00390000010868"/>
    <dbReference type="HOGENOM" id="CLU_088713_1_0_1"/>
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    <dbReference type="OMA" id="FYKASNP"/>
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        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061952">
        <property type="term" value="P:midbody abscission"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02683">
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        <property type="match status" value="1"/>
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        <property type="entry name" value="MITD, C-terminal phospholipase D-like domain"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="1.20.58.80">
        <property type="entry name" value="Phosphotransferase system, lactose/cellobiose-type IIA subunit"/>
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        <property type="entry name" value="MIT_domain_contain_protein1"/>
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    <dbReference type="InterPro" id="IPR045331">
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        <property type="match status" value="1"/>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="9"/>
            <end position="85"/>
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    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="59"/>
            <end position="86"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00745"/>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
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</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>F6YN50</accession>
    <name>F6YN50_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transcription factor protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">nf-il2</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">wild type</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bZIP family. NFIL3 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002192">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6YN50"/>
    <dbReference type="IntAct" id="F6YN50">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000016562"/>
    <dbReference type="Ensembl" id="ENSCINT00000016562.3">
        <property type="protein sequence ID" value="ENSCINP00000016562.3"/>
        <property type="gene ID" value="ENSCING00000008105.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000169039"/>
    <dbReference type="HOGENOM" id="CLU_466133_0_0_1"/>
    <dbReference type="InParanoid" id="F6YN50"/>
    <dbReference type="OMA" id="CYTWPED"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007623">
        <property type="term" value="P:circadian rhythm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14694">
        <property type="entry name" value="bZIP_NFIL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000025">
        <property type="entry name" value="nuclear factor interleukin-3-regulated protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047229">
        <property type="entry name" value="NFIL3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047106">
        <property type="entry name" value="NFIL3-like_bZIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15284:SF0">
        <property type="entry name" value="GH23983P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15284">
        <property type="entry name" value="NUCLEAR FACTOR INTERLEUKIN-3-REGULATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07716">
        <property type="entry name" value="bZIP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="7">
        <location>
            <begin position="17"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="322"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="322"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="347"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000016562.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="585" mass="66656" checksum="64D0E530C7A1F919" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>F6YNJ1</accession>
    <name>F6YNJ1_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">wild type</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the heat shock protein 90 family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001812">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="EAAA01001813">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6YNJ1"/>
    <dbReference type="FunCoup" id="F6YNJ1">
        <property type="interactions" value="511"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000023416"/>
    <dbReference type="Ensembl" id="ENSCINT00000023662.2">
        <property type="protein sequence ID" value="ENSCINP00000023416.2"/>
        <property type="gene ID" value="ENSCING00000012576.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01020000230401"/>
    <dbReference type="HOGENOM" id="CLU_006684_1_3_1"/>
    <dbReference type="InParanoid" id="F6YNJ1"/>
    <dbReference type="OMA" id="EMFFEIS"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034605">
        <property type="term" value="P:cellular response to heat"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="entry name" value="Heat shock protein 90 alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000357">
        <property type="entry name" value="Heat shock protein HSP 90-beta"/>
        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.790">
        <property type="entry name" value="Heat shock protein 90, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019805">
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    <dbReference type="InterPro" id="IPR020575">
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    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003555">
        <property type="entry name" value="PRK05218.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11528">
        <property type="entry name" value="HEAT SHOCK PROTEIN 90 FAMILY MEMBER"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00183">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00775">
        <property type="entry name" value="HEATSHOCK90"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF110942">
        <property type="entry name" value="HSP90 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00298">
        <property type="entry name" value="HSP90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0143">Chaperone</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0346">Stress response</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="637"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="193"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="637"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="123"/>
            <end position="124"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="333"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR002583-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000023416.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="666" mass="77018" checksum="A40EE7216226386A" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>F6YX18</accession>
    <name>F6YX18_CIOIN</name>
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        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">wild type</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002581">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6YX18"/>
    <dbReference type="STRING" id="7719.ENSCINP00000024552"/>
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        <property type="protein sequence ID" value="ENSCINP00000024552.2"/>
        <property type="gene ID" value="ENSCING00000004832.3"/>
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    <dbReference type="InParanoid" id="F6YX18"/>
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        <property type="component" value="Chromosome 8"/>
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    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0007417">
        <property type="term" value="P:central nervous system development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0034653">
        <property type="term" value="P:retinoic acid catabolic process"/>
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        <property type="project" value="GO_Central"/>
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    <dbReference type="Gene3D" id="1.10.630.10">
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        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
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    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
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        <property type="entry name" value="Cyt_P450_E_grp-IV"/>
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        <property type="match status" value="1"/>
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    </dbReference>
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        <location>
            <begin position="41"/>
            <end position="66"/>
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    <feature type="binding site" description="axial binding residue" evidence="7">
        <location>
            <position position="474"/>
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        <ligand>
            <name>heme</name>
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        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
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        <source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR602403-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000024552.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="524" mass="58803" checksum="C58DB82D4C529590" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F6ZF87</accession>
    <name>F6ZF87_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">GLIPR1-like protein 1</fullName>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
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                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
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                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
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                <person name="Zhang C."/>
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                <person name="Larimer F."/>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
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        </citation>
        <scope>IDENTIFICATION</scope>
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                <person name="Terry A."/>
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                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
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                <person name="De Tomaso A."/>
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                <person name="Yoshizaki F."/>
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                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
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                <person name="Detter C."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
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                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the sulfotransferase 1 family.</text>
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    <dbReference type="EC" id="2.8.2.-" evidence="3"/>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="GeneTree" id="ENSGT00940000163815"/>
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        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
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            <begin position="70"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
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    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
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    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
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    </evidence>
    <sequence length="329" mass="39107" checksum="CD8CD2F8D193FC9E" modified="2011-07-27" version="1">MEWVEESYRRSFKEISDFGKDVPDIQPLIDYFVESLTQPKLTEWKGYKVYGFGFNADTIKWVYDNWTPWQDDVIVASFPKTGTTWLRHIVRLLYFRHDENLLKMTEGMVMPHIYLETGIPLKFEFLDKLPWDRKVLSTHVPAALFNFEKIRNAGAKVIYTIRNPKDQLVSWHKMMVNFPINREGTNLKDHYPLEWGKFFDVAISGKQQMTNKEGEYYMEHLLSWYPHRNDENVLFVVFEDLKKDPLKEIRRIAKFLDVKISEDDLLNVTESSSFASMKKEYHWTEKVKFFRKGEVGDWKNHFTVAQSEKVDALVKQKLAGTDIKFTYEL</sequence>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01001701">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="RefSeq" id="XP_002131006.3">
        <property type="nucleotide sequence ID" value="XM_002130970.3"/>
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    <dbReference type="AlphaFoldDB" id="F6ZQF4"/>
    <dbReference type="Ensembl" id="ENSCINT00000023927.2">
        <property type="protein sequence ID" value="ENSCINP00000023681.2"/>
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    <dbReference type="GeneID" id="100177091"/>
    <dbReference type="KEGG" id="cin:100177091"/>
    <dbReference type="GeneTree" id="ENSGT00730000113521"/>
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    <dbReference type="InParanoid" id="F6ZQF4"/>
    <dbReference type="OMA" id="GLDCIQC"/>
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        <property type="component" value="Chromosome 3"/>
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        <property type="entry name" value="Snake_toxin-like_sf"/>
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    <dbReference type="SUPFAM" id="SSF57302">
        <property type="entry name" value="Snake toxin-like"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5030169757" evidence="1">
        <location>
            <begin position="18"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000023681.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    </evidence>
    <sequence length="139" mass="13933" checksum="E25227D41EFB200E" modified="2012-04-18" version="2">MYRIVIAVLLLANQGFALNCYQCGLTTETGSCSTGSISAANLQACPAGFNYCTTLSLINQNGGGSSTTTRGCSATPQAHSCIGAFGFYSCTGATCATDGCNSGTVQFVTPTGGGAGKKEATILAVVLSSILSFVVCSLN</sequence>
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<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>F6ZSK5</accession>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
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                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="ENSCINP00000019894.3"/>
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    <dbReference type="GeneID" id="113474829"/>
    <dbReference type="KEGG" id="cin:113474829"/>
    <dbReference type="GeneTree" id="ENSGT00440000035991"/>
    <dbReference type="HOGENOM" id="CLU_836664_0_0_1"/>
    <dbReference type="InParanoid" id="F6ZSK5"/>
    <dbReference type="OMA" id="CAICKEF"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 13"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
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    <feature type="chain" id="PRO_5030169762" evidence="2">
        <location>
            <begin position="19"/>
            <end position="332"/>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="302"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="304"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000019894.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="332" mass="35007" checksum="EE610B542355AABA" modified="2011-07-27" version="1">MKRLIVLFALVFITGATGYGFRHWPWYFPTVPPPVCEFCFSSDKCVGPADNLNAACKTCLNTYCASTITNLGQVGSSADCIARAWPTYCSASCGVYSAARTDKCVNCVRACKTKFDAMIPAKCPAITGDPCLSCESATACLTEAEVRNLTLTKPVCHQYVGSLCIQNAPVLTSACLISAWTNAASGCGVLTTAMRNRFTACGTCQAACQRAQTCARNCTTMTTYPTTIPCAACRVNCNGTTTACAPGACDPGCAPCDACFKNCEIGSFQIQNPCNCPSGRIGRRYGGYFGGGGYRGGYGYGRRGGGRRGGGRRGRGRGGRRGRGRGGRRGRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>F6ZZA5</accession>
    <name>F6ZZA5_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="F6ZZA5"/>
    <dbReference type="Ensembl" id="ENSCINT00000000542.3">
        <property type="protein sequence ID" value="ENSCINP00000000542.3"/>
        <property type="gene ID" value="ENSCING00000000294.3"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_180895_3_0_1"/>
    <dbReference type="InParanoid" id="F6ZZA5"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003347253" evidence="1">
        <location>
            <begin position="24"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000000542.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="63" mass="7551" checksum="CC7D6D04BA0830C4" modified="2011-07-27" version="1">MNKSALLLLLLIGLFVLAETTDAMYIWGRRTWGVNRRRRRRWNQQKEAEDIEESDEQVMSLLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>F6ZZG0</accession>
    <name>F6ZZG0_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cep57 centrosome localisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">wild type</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the translokin family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002273">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6ZZG0"/>
    <dbReference type="FunCoup" id="F6ZZG0">
        <property type="interactions" value="171"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000012510"/>
    <dbReference type="Ensembl" id="ENSCINT00000012510.3">
        <property type="protein sequence ID" value="ENSCINP00000012510.3"/>
        <property type="gene ID" value="ENSCING00000006051.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063695"/>
    <dbReference type="HOGENOM" id="CLU_034321_2_0_1"/>
    <dbReference type="InParanoid" id="F6ZZG0"/>
    <dbReference type="OMA" id="TQNNAEM"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043015">
        <property type="term" value="F:gamma-tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051756">
        <property type="entry name" value="Centrosomal_MT-associated"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025913">
        <property type="entry name" value="Cep57_CLD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024957">
        <property type="entry name" value="Cep57_MT-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19336:SF9">
        <property type="entry name" value="SPINDLE POLE BODY PROTEIN PPC89"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19336">
        <property type="entry name" value="UNCHARACTERIZED DUF1167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14073">
        <property type="entry name" value="Cep57_CLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06657">
        <property type="entry name" value="Cep57_MT_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cep57 centrosome localisation" evidence="10">
        <location>
            <begin position="47"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="Cep57 centrosome microtubule-binding" evidence="9">
        <location>
            <begin position="350"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="24"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="231"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="332"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="410"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="52"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="118"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="245"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF06657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF14073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000012510.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="503" mass="57594" checksum="882D3A6321602123" modified="2012-04-18" version="2">MATSLFDEPPSIASFQDYPTTRPFINSNLRRSPGKPVKAYPESSSKAVLSALKGLQDKIYNLELDRSKAEKNLKHLAAETSLYKASPVLNSRNKSNESLHNDTSEKVLMTPTEVESQLRNADTRCRLLERQLENMRRMVQCAEKERSDALERQVALERERGRVTAENREAQAKLEQLQKLEHRFEDLASRKNKSEVKVKELEEKLKLEEHQRKLLCDKAAQFQTEAEKNRILYQHEIPERDTNTKPKKKSTKKKKKANANGVKTKKPAVVKRPAKSAGQPHYRLNLADVPFIAGTSTSPSHAVSANFQRLLHDLKHHSPLYCNDNVVSERKISTGSSTTEEEDTRGRQRVHQSSVEEDDLTDLLVALQDEFGKMTFEHQVLAKQITSTQDRQTKREFELEMENLVSRMERKGEQISTVRRHRAQLSENRRNNSGKGKHPTHGVRSTSDPRGFRRPRSANPTSVGNSPMAPRVPPGEMRKKRLAFLKEMKTVRSNLRKDDLSWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>F7ADR7</accession>
    <name>F7ADR7_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Long-chain specific acyl-CoA dehydrogenase, mitochondrial</fullName>
            <ecNumber evidence="6">1.3.8.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="35" type="primary">LOC100180056</name>
    </gene>
    <organism evidence="35 36">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="36" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="35" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="35">wild type</strain>
        </source>
    </reference>
    <reference evidence="35" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="35" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Long-chain specific acyl-CoA dehydrogenase is one of the acyl-CoA dehydrogenases that catalyze the first step of mitochondrial fatty acid beta-oxidation, an aerobic process breaking down fatty acids into acetyl-CoA and allowing the production of energy from fats. The first step of fatty acid beta-oxidation consists in the removal of one hydrogen from C-2 and C-3 of the straight-chain fatty acyl-CoA thioester, resulting in the formation of trans-2-enoyl-CoA. Among the different mitochondrial acyl-CoA dehydrogenases, long-chain specific acyl-CoA dehydrogenase can act on saturated and unsaturated acyl-CoAs with 6 to 24 carbons with a preference for 8 to 18 carbons long primary chains.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>(5Z)-tetradecenoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E,5Z)-tetradecadienoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:47448"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:84650"/>
            <dbReference type="ChEBI" id="CHEBI:87701"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:47449"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>a long-chain 2,3-saturated fatty acyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = a long-chain (2E)-enoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:17721"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:83721"/>
            <dbReference type="ChEBI" id="CHEBI:83727"/>
            <dbReference type="EC" id="1.3.8.8"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:17722"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>decanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-decenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:48176"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:61406"/>
            <dbReference type="ChEBI" id="CHEBI:61430"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:48177"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>docosanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-docosenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:47228"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:65059"/>
            <dbReference type="ChEBI" id="CHEBI:74692"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:47229"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>dodecanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-dodecenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:47296"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57330"/>
            <dbReference type="ChEBI" id="CHEBI:57375"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:47297"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>eicosanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-eicosenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:47236"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57380"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:74691"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="28">
            <dbReference type="Rhea" id="RHEA:47237"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>hexanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-hexenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:43464"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:62077"/>
            <dbReference type="ChEBI" id="CHEBI:62620"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="29">
            <dbReference type="Rhea" id="RHEA:43465"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="30">
            <text>octadecanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-octadecenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:47240"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57394"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:71412"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="30">
            <dbReference type="Rhea" id="RHEA:47241"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>octanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-octenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:48180"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57386"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:62242"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:48181"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>oxidized [electron-transfer flavoprotein] + (9Z)-octadecenoyl-CoA + H(+) = (2E,9Z)-octadecadienoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:47300"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57387"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:77553"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:47301"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>oxidized [electron-transfer flavoprotein] + hexadecanoyl-CoA + H(+) = (2E)-hexadecenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:43448"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:61526"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:43449"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>tetracosanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-tetracosenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:47232"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:65052"/>
            <dbReference type="ChEBI" id="CHEBI:74693"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:47233"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>tetradecanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-tetradecenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:47316"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57385"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:61405"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="27">
            <dbReference type="Rhea" id="RHEA:47317"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 31">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism; mitochondrial fatty acid beta-oxidation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 31">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.8.8" evidence="6"/>
    <dbReference type="EMBL" id="EAAA01002978">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_026691638.1">
        <property type="nucleotide sequence ID" value="XM_026835837.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_026691639.1">
        <property type="nucleotide sequence ID" value="XM_026835838.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F7ADR7"/>
    <dbReference type="STRING" id="7719.ENSCINP00000019524"/>
    <dbReference type="Ensembl" id="ENSCINT00000019524.3">
        <property type="protein sequence ID" value="ENSCINP00000019524.3"/>
        <property type="gene ID" value="ENSCING00000009605.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100180056"/>
    <dbReference type="GeneTree" id="ENSGT00940000157652"/>
    <dbReference type="HOGENOM" id="CLU_018204_0_3_1"/>
    <dbReference type="InParanoid" id="F7ADR7"/>
    <dbReference type="OMA" id="MWEYPVA"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004466">
        <property type="term" value="F:long-chain fatty acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019254">
        <property type="term" value="P:carnitine metabolic process, CoA-linked"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033539">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA dehydrogenase"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042758">
        <property type="term" value="P:long-chain fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.110.10:FF:000002">
        <property type="entry name" value="Acyl-CoA dehydrogenase fadE12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.540.10:FF:000017">
        <property type="entry name" value="long-chain specific acyl-CoA dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.10:FF:000020">
        <property type="entry name" value="Long-chain specific acyl-CoA dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050741">
        <property type="entry name" value="Acyl-CoA_dehydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006089">
        <property type="entry name" value="Acyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48083:SF20">
        <property type="entry name" value="LONG-CHAIN SPECIFIC ACYL-COA DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48083">
        <property type="entry name" value="MEDIUM-CHAIN SPECIFIC ACYL-COA DEHYDROGENASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00072">
        <property type="entry name" value="ACYL_COA_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00073">
        <property type="entry name" value="ACYL_COA_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0007">Acetylation</keyword>
    <keyword evidence="10 31" id="KW-0274">FAD</keyword>
    <keyword evidence="11" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="9 31" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="15" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="16" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="14 31" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="36" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase N-terminal" evidence="34">
        <location>
            <begin position="42"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="33">
        <location>
            <begin position="157"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="32">
        <location>
            <begin position="269"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00014123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00045155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00047893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00047916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00048020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00048086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00048187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00048725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00048877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00049038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00049140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00049192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00049224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="32">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="33">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="34">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="35">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000019524.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="36">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="421" mass="47163" checksum="E5D7748BF47D7CCF" modified="2012-04-18" version="2">MKLRPGQINRLFSTSSAAASEFRKEGFQSGRFTDIGTRQIFNEDHDMFRESVRKFYQEEVLPYHDQWEEEGQVSRELWLKAGDMGILGVNTPEEHGGIGGDWLSAAIVHEEQGYVNCSGPGFALHSDIVMRYITKYGTPEQIEKYIPDMTAGKKIGAIAMTEPGAGSDLQGVRTFAKKDGDDWVLNGSKVFITNGWMADVVIVVAITDKEAKSAAHGISLFLVDGDNPGFIKGKKLKKMGLRAQDTAELFFEDCRLPSSALLGGKAGLNAGFYQLMQELPQERLLISNLCMAACEFMFEETRDYVKQRKAFGKTLSKLQTIKHTLAEIKTETAVARGFLDRCNDLHNQRKLDTSTASMAKYYVTDLNNTVAYRCVQLHGGWGYMWEYPICKAYVDARVQTIYGGSNEIMKELISRDIEKDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>F7AH61</accession>
    <name>F7AH61_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12 14">Uracil-DNA glycosylase</fullName>
            <shortName evidence="12">UDG</shortName>
            <ecNumber evidence="12 14">3.2.2.27</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">wild type</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12 14">Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19 12 14">
            <text>Hydrolyzes single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil.</text>
            <dbReference type="EC" id="3.2.2.27"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a 2'-deoxyuridine in double-stranded DNA + H2O = a 2'-deoxyribose 5'-monophosphate in double-stranded DNA + uracil</text>
            <dbReference type="Rhea" id="RHEA:81455"/>
            <dbReference type="Rhea" id="RHEA-COMP:14231"/>
            <dbReference type="Rhea" id="RHEA-COMP:17071"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17568"/>
            <dbReference type="ChEBI" id="CHEBI:133902"/>
            <dbReference type="ChEBI" id="CHEBI:139095"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:81456"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a 2'-deoxyuridine in single-stranded DNA + H2O = a 2'-deoxyribose 5'-monophosphate in single-stranded DNA + uracil</text>
            <dbReference type="Rhea" id="RHEA:81459"/>
            <dbReference type="Rhea" id="RHEA-COMP:12847"/>
            <dbReference type="Rhea" id="RHEA-COMP:19684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17568"/>
            <dbReference type="ChEBI" id="CHEBI:133902"/>
            <dbReference type="ChEBI" id="CHEBI:139095"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:81460"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Interacts with RPA2 subunit of the RPA trimer; this interaction mediates UNG2 recruitment to RPA-coated single-stranded DNA at stalled replication forks. Interacts with PCNA; this interaction mediates UNG2 recruitment to S-phase replication foci. Interacts (via N-terminus) with FAM72A.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 12 14">Belongs to the uracil-DNA glycosylase (UDG) superfamily. UNG family.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.27" evidence="12 14"/>
    <dbReference type="EMBL" id="EAAA01002293">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F7AH61"/>
    <dbReference type="FunCoup" id="F7AH61">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000005659"/>
    <dbReference type="Ensembl" id="ENSCINT00000005659.3">
        <property type="protein sequence ID" value="ENSCINP00000005659.3"/>
        <property type="gene ID" value="ENSCING00000002767.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000003405"/>
    <dbReference type="HOGENOM" id="CLU_032162_2_1_1"/>
    <dbReference type="InParanoid" id="F7AH61"/>
    <dbReference type="OMA" id="PDNGYLM"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004844">
        <property type="term" value="F:uracil DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097510">
        <property type="term" value="P:base-excision repair, AP site formation via deaminated base removal"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd10027">
        <property type="entry name" value="UDG-F1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.470.10:FF:000004">
        <property type="entry name" value="Uracil-DNA glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.470.10">
        <property type="entry name" value="Uracil-DNA glycosylase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00148">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002043">
        <property type="entry name" value="UDG_fam1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018085">
        <property type="entry name" value="Ura-DNA_Glyclase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005122">
        <property type="entry name" value="Uracil-DNA_glycosylase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036895">
        <property type="entry name" value="Uracil-DNA_glycosylase-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003588">
        <property type="entry name" value="PRK05254.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003589">
        <property type="entry name" value="PRK05254.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003591">
        <property type="entry name" value="PRK05254.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003592">
        <property type="entry name" value="PRK05254.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00628">
        <property type="entry name" value="ung"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11264">
        <property type="entry name" value="URACIL-DNA GLYCOSYLASE"/>
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        <location>
            <begin position="109"/>
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        <location>
            <begin position="1"/>
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        </location>
    </feature>
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        <location>
            <begin position="1"/>
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        </location>
    </feature>
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        <location>
            <position position="124"/>
        </location>
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        <source>
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        <source>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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        <source>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">wild type</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="ENSCING00000012263.2"/>
    </dbReference>
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        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000022981.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="75" mass="9082" checksum="7F6F2CF56136AD0B" modified="2012-04-18" version="2">MVKNIAKILENICLSCSSVGSCWCYNWDLLHCEQQWIQVRRIIWLIIQTSYHLYIMFGPKKLWSLKRWLSLYSTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>F7ANP5</accession>
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        <recommendedName>
            <fullName evidence="3">Carboxylic ester hydrolase</fullName>
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    <organism evidence="5 6">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
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                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
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                <person name="Hayashi H."/>
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                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
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                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
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                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
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                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
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            </authorList>
            <dbReference type="PubMed" id="18854010"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">wild type</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="1 3">Belongs to the type-B carboxylesterase/lipase family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="ENSCINP00000015297.3"/>
        <property type="gene ID" value="ENSCING00000007447.3"/>
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        <property type="component" value="Chromosome 2"/>
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    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="CarbesteraseB"/>
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        <property type="entry name" value="Carboxylesterase_B_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019819">
        <property type="entry name" value="Carboxylesterase_B_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45237">
        <property type="entry name" value="POSSIBLE PARA-NITROBENZYL ESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45237:SF2">
        <property type="entry name" value="POSSIBLE PARA-NITROBENZYL ESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="COesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00122">
        <property type="entry name" value="CARBOXYLESTERASE_B_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00941">
        <property type="entry name" value="CARBOXYLESTERASE_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carboxylesterase type B" evidence="4">
        <location>
            <begin position="4"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000015297.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="245" mass="26288" checksum="557FEEB8ADB0D60C" modified="2012-04-18" version="2">MTECPTVTTAYGKVRGKVLKSLGVSGSKEVYKFAGVPFAKPPVGALRFEPPQKPDSWDGVKETTKSGPIPMQDARANAKLLPYLMAHVDALETGFERSEDCLYLDVYTPSTSDSSSLPVMVWIYGGGLQNGFGNIYHAHCLCALYDVVVVVPTYRVNICGFLTLGADTKWPGNIGMLDQVAALKWTRDNVKGFGGDPSNVTIFGESAGAFSVGLHVVSPMSKGLFHRAISHSGTAHFPVLLSRSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>F7AVJ2</accession>
    <name>F7AVJ2_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NACHT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01002045">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F7AVJ2"/>
    <dbReference type="Ensembl" id="ENSCINT00000023297.2">
        <property type="protein sequence ID" value="ENSCINP00000023051.2"/>
        <property type="gene ID" value="ENSCING00000012314.2"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1590120_0_0_1"/>
    <dbReference type="InParanoid" id="F7AVJ2"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051191">
        <property type="entry name" value="DCAF12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19860">
        <property type="entry name" value="DDB1- AND CUL4-ASSOCIATED FACTOR 12-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19860:SF18">
        <property type="entry name" value="DUF4062 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000023051.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="168" mass="19245" checksum="EC8339CADC9C3005" modified="2012-04-18" version="2">MRRCIKTLREATWKTKCTSSEMLEDVDTYKGLVETFQSSLSLHPCVVVIDSVNDIKYGMHEDIGQVKGLGWLPVVIPLTCHIIISTRRSDLTYKAIQQRGDVTIVKMPSIDLQTRENILQTYFEDNVVNILGRDHLSKVWANRLSERPLYLATLSHLIMSCRDSHDIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>F7AZA1</accession>
    <accession>A0A1W2WC58</accession>
    <name>F7AZA1_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">N-alpha-acetyltransferase 20</fullName>
            <ecNumber evidence="5">2.3.1.254</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Methionine N-acetyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">N-acetyltransferase 5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">N-terminal acetyltransferase B complex catalytic subunit NAA20</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">N-terminal acetyltransferase B complex catalytic subunit NAT5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">NatB catalytic subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC100179502</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Catalytic subunit of the NatB complex which catalyzes acetylation of the N-terminal methionine residues of peptides beginning with Met-Asp, Met-Glu, Met-Asn and Met-Gln. Proteins with cell cycle functions are overrepresented in the pool of NatB substrates. Required for maintaining the structure and function of actomyosin fibers and for proper cellular migration.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>N-terminal L-methionyl-L-asparaginyl-[protein] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-methionyl-L-asparaginyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50484"/>
            <dbReference type="Rhea" id="RHEA-COMP:12694"/>
            <dbReference type="Rhea" id="RHEA-COMP:12695"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:133356"/>
            <dbReference type="ChEBI" id="CHEBI:133358"/>
            <dbReference type="EC" id="2.3.1.254"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>N-terminal L-methionyl-L-aspartyl-[protein] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-methionyl-L-aspartyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50480"/>
            <dbReference type="Rhea" id="RHEA-COMP:12692"/>
            <dbReference type="Rhea" id="RHEA-COMP:12693"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:133045"/>
            <dbReference type="ChEBI" id="CHEBI:133063"/>
            <dbReference type="EC" id="2.3.1.254"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>N-terminal L-methionyl-L-glutaminyl-[protein] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-methionyl-L-glutaminyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50492"/>
            <dbReference type="Rhea" id="RHEA-COMP:12698"/>
            <dbReference type="Rhea" id="RHEA-COMP:12699"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:133361"/>
            <dbReference type="ChEBI" id="CHEBI:133362"/>
            <dbReference type="EC" id="2.3.1.254"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>N-terminal L-methionyl-L-glutamyl-[protein] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-methionyl-L-glutamyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50488"/>
            <dbReference type="Rhea" id="RHEA-COMP:12696"/>
            <dbReference type="Rhea" id="RHEA-COMP:12697"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:133359"/>
            <dbReference type="ChEBI" id="CHEBI:133360"/>
            <dbReference type="EC" id="2.3.1.254"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the N-terminal acetyltransferase B (NatB) complex which is composed of NAA20 and NAA25.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the acetyltransferase family. ARD1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.254" evidence="5"/>
    <dbReference type="RefSeq" id="XP_002128688.1">
        <property type="nucleotide sequence ID" value="XM_002128652.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F7AZA1"/>
    <dbReference type="FunCoup" id="F7AZA1">
        <property type="interactions" value="420"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000021531"/>
    <dbReference type="Ensembl" id="ENSCINT00000021531.3">
        <property type="protein sequence ID" value="ENSCINP00000021531.3"/>
        <property type="gene ID" value="ENSCING00000010954.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100179502"/>
    <dbReference type="KEGG" id="cin:100179502"/>
    <dbReference type="GeneTree" id="ENSGT00550000075046"/>
    <dbReference type="HOGENOM" id="CLU_013985_7_1_1"/>
    <dbReference type="InParanoid" id="F7AZA1"/>
    <dbReference type="OMA" id="EQHPSMR"/>
    <dbReference type="OrthoDB" id="10264728at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031416">
        <property type="term" value="C:NatB complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120518">
        <property type="term" value="F:protein N-terminal-methionine acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004596">
        <property type="term" value="F:protein-N-terminal amino-acid acetyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032956">
        <property type="term" value="P:regulation of actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000065">
        <property type="entry name" value="N-terminal acetyltransferase complex ARD1 subunit homolog"/>
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        <property type="entry name" value="NatB_acetyltransferase_subunit"/>
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    <dbReference type="PANTHER" id="PTHR45910">
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    <dbReference type="PANTHER" id="PTHR45910:SF1">
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        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="1" id="KW-0808">Transferase</keyword>
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            <end position="156"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51186"/>
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    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000021531.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
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</entry>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
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            <begin position="41"/>
            <end position="67"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000026973.2"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
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            <fullName evidence="2">Uncharacterized protein</fullName>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
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                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
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                <person name="Miura S."/>
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                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01002659">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F7BMI8"/>
    <dbReference type="Ensembl" id="ENSCINT00000024676.2">
        <property type="protein sequence ID" value="ENSCINP00000024430.2"/>
        <property type="gene ID" value="ENSCING00000003381.3"/>
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    <dbReference type="GeneTree" id="ENSGT00390000010270"/>
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        <property type="component" value="Chromosome 8"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
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            <begin position="315"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000024430.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="349" mass="39136" checksum="11C24897F78699BD" modified="2012-04-18" version="2">MNFNRDSSVYNRRRAFMNRSCSGYLKGGITSTSKDSLDDDIDDATSETSSVQSTNSTRSYKSWRQAHKRRRREKLERTQSLFGDATSRDYREERRRQIDPFTFDIRRTPIHSMEGSVDPGLKEDSRNISSSSVDIDIPRHDVTTDNYDVGQFNELSTIIQSPVEPFYSSSSLDSLSEGESGKPKISEDDVIRHQKWPTSEEHKETRDWLISKSSPPPQNEYVIKKIPNVIEKSKEITKRSKEPNLALAFGAGGLNELSRIFESRQATGEHSEVFNQISDVNNSVDISIPKVISSTPSYLEQPASNGPASTVPGAEDGKTEEKDSDVLHGQGNEHPEKQQPNEGAGHISP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>H2XKQ3</accession>
    <name>H2XKQ3_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
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        <lineage>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
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                <person name="Satou Y."/>
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                <person name="Chapman J."/>
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                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
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                <person name="Hastings K.E."/>
                <person name="Ho I."/>
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                <person name="Huang W."/>
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                <person name="Putnam N."/>
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                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
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                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
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                <person name="Detter C."/>
                <person name="Doggett N."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Ueno K."/>
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                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Yamada L."/>
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        <property type="term" value="F:carbohydrate binding"/>
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        <property type="term" value="F:pattern recognition receptor activity"/>
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        <property type="term" value="P:immune response"/>
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    <dbReference type="CDD" id="cd00037">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
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    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
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        <property type="entry name" value="C-type_lectin/snaclec_domain"/>
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        <property type="entry name" value="C-type_lectin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22803">
        <property type="entry name" value="MANNOSE, PHOSPHOLIPASE, LECTIN RECEPTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="C_TYPE_LECTIN_1"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5030172489" evidence="3">
        <location>
            <begin position="19"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="4">
        <location>
            <begin position="25"/>
            <end position="142"/>
        </location>
    </feature>
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        <location>
            <begin position="91"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="91"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000030886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="242" mass="27304" checksum="1D0E2E43245F371E" modified="2012-04-18" version="1">MKLFAFCCILSCLSIAVGQITWHQLGTSEYYIHDGTAVNHSAAVNACASMNATLARVKTTQVQQFLVQNKKKRTSYYIGLERINPTQTGFQWNDGTSVTSGETQWSGTGSNPDNSGGRENCVQLSKQWNDISCGTTMKYICERHADIIANGSSVKVVSSTYNSYTMLDVMYKMCFDTAPYKVEASVRMLHESLPYRFNVIPTVVQPHFFRIKLERVDIYSGWSPSEIKISWKAYFNTTVQIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
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            <fullName evidence="2">Uncharacterized protein</fullName>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Ciona</taxon>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01001424">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XNB3"/>
    <dbReference type="Ensembl" id="ENSCINT00000031043.1">
        <property type="protein sequence ID" value="ENSCINP00000031146.1"/>
        <property type="gene ID" value="ENSCING00000019898.1"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1163381_0_0_1"/>
    <dbReference type="InParanoid" id="H2XNB3"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="133"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="133"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000031146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="239" mass="27233" checksum="F34EC0AD9C8975FF" modified="2012-04-18" version="1">MEHLTRDEVTLYNDSGTSELEKLKILNCMVDRLLVDRENGKHGSSLDAKLHHQQQISTYKQSDIKRIPVEAVAGEDHWGAKKYLNSGFDFVNEYFAQSDELSTKTNSMKHVLASHKSSYQVVQSNNELFQTPKNETKVNMVSSEGRSESDNDSDGSDVESVKSTLQTHRPCYEPQNSELVVFSPEIITREIHELEITIKKDHKRTPLYSEAENYKLHDEYSTPMHLNIEASTTAVDNLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>H2XP47</accession>
    <name>H2XP47_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">G protein gamma domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">wild type</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G protein gamma family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002729">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XP47"/>
    <dbReference type="STRING" id="7719.ENSCINP00000031430"/>
    <dbReference type="Ensembl" id="ENSCINT00000035921.1">
        <property type="protein sequence ID" value="ENSCINP00000031430.1"/>
        <property type="gene ID" value="ENSCING00000018384.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01100000263525"/>
    <dbReference type="HOGENOM" id="CLU_168377_3_1_1"/>
    <dbReference type="InParanoid" id="H2XP47"/>
    <dbReference type="OMA" id="FCIQNAN"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005834">
        <property type="term" value="C:heterotrimeric G-protein complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031681">
        <property type="term" value="F:G-protein beta-subunit binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00068">
        <property type="entry name" value="GGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.260.10:FF:000001">
        <property type="entry name" value="Guanine nucleotide-binding protein subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.260.10">
        <property type="entry name" value="Transducin (heterotrimeric G protein), gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001770">
        <property type="entry name" value="G-protein_gamma"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015898">
        <property type="entry name" value="G-protein_gamma-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036284">
        <property type="entry name" value="GGL_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13809">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN GAMMA SUBUNIT"/>
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        <source>
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    <accession>H2XQ22</accession>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">wild type</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="Ensembl" id="ENSCINT00000032422.1">
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        <property type="gene ID" value="ENSCING00000021725.1"/>
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        <property type="component" value="Chromosome 8"/>
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    </feature>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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</entry>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">wild type</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01000789">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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            <taxon>Tunicata</taxon>
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            <taxon>Phlebobranchia</taxon>
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            <taxon>Ciona</taxon>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="H2XS85"/>
    <dbReference type="Ensembl" id="ENSCINT00000034162.1">
        <property type="protein sequence ID" value="ENSCINP00000032519.1"/>
        <property type="gene ID" value="ENSCING00000024263.1"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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        <source>
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    </evidence>
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        <source>
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    <sequence length="81" mass="8979" checksum="18E5594F2B1C62A2" modified="2012-04-18" version="1">MSLLIDIVLDVAPLEMDTIQENGLYYVLSLALKEMSFIKECSSCKTAFISSDENELLQRAAAVLTCLKSHTANHNLSPMMI</sequence>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
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                <person name="Haga S."/>
                <person name="Hayashi H."/>
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                <person name="Inaba K."/>
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                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">wild type</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="H2XSB1"/>
    <dbReference type="Ensembl" id="ENSCINT00000036764.1">
        <property type="protein sequence ID" value="ENSCINP00000032545.1"/>
        <property type="gene ID" value="ENSCING00000022171.1"/>
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        <property type="component" value="Chromosome 4"/>
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    <proteinExistence type="predicted"/>
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    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000032545.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    </evidence>
    <sequence length="27" mass="3514" checksum="B1B376C339F2921D" modified="2012-04-18" version="1">MFFVYYKTRQEIRIKMCPIFLHFILYL</sequence>
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<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>H2XSX9</accession>
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        <recommendedName>
            <fullName evidence="8">Elongation factor 1-alpha</fullName>
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    <organism evidence="10 11">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ciona</taxon>
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    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
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                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
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                <person name="Kobayashi K."/>
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                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
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                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
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                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
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                <person name="Shin-i T."/>
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                <person name="Suzuki M.M."/>
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                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="10">wild type</strain>
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    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <text evidence="8">This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.</text>
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            <location evidence="1">Cytoplasm</location>
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    <comment type="similarity">
        <text evidence="2 8">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
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        <source>
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    <sequence length="460" mass="50583" checksum="800D4EF6B21F9DE9" modified="2012-04-18" version="1">KMKDKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRAIEKFEKEAAEMGKGSFKYAWVLDKLKAERERGITIDIALWKFETNNYSITIIDAPGHRDFIKNMITGTSQADCAVLIVAAGVGEFEAGISKNGQTREHALLAFTLGVKQIIVAVNKMDSTEPKYSQKRFEEISKEVTTYLKKVGYNPKAVAFVPISGWHGDNMLEESENMSWYKGWSREQDKKTLTGKTLFNALDSIAPPKRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGILKPGVVATFAPVNLSTEVKSVEMHHESMTEALPGDNVGFNVKNVSVKDIKRGYVAGDSKNDPPKQAETFTAQVIILNHPGQIHAGYAPVLDCHTAHIACKFQELKEKIDRRSGKKLEDAPKAVKSGDAAIVVLKPQKPMCVEAFQEYPPLGRFAVRDMRNTVAVGVIKSVEKKEVAGKETKSAKKVGKK</sequence>
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    <accession>H2XT33</accession>
    <name>H2XT33_CIOIN</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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            <taxon>Eukaryota</taxon>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="ENSCINP00000032817.1"/>
        <property type="gene ID" value="ENSCING00000021522.1"/>
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            <begin position="1"/>
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            <begin position="1"/>
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        <location>
            <begin position="128"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="142"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000032817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="162" mass="18457" checksum="EECBE8423662EDCB" modified="2012-04-18" version="1">MRNEEPQMKKTKWDDAGNYRDDPSESANMEAAFTATASATSNRRNSNLITLSTQDSEPIPTTASAPSATETPSKNRFNPISHKDSDDKNSWHSETLKRKPQSSYNEYSDSNPTHQFQNFAPTPELPKHQSQNGSQYKNQQESYHEHHEPAEEYRPSSSSHHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>H2XUA8</accession>
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        <recommendedName>
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        <name type="scientific">Ciona intestinalis</name>
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    <reference evidence="5" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
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                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
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                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
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                <person name="Hayashi H."/>
                <person name="Hino K."/>
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                <person name="Inaba K."/>
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                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
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                <person name="Manohar C."/>
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                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
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                <person name="Miura S."/>
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                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
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                <person name="Sasaki A."/>
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                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="H2XUA8"/>
    <dbReference type="Ensembl" id="ENSCINT00000032499.1">
        <property type="protein sequence ID" value="ENSCINP00000033242.1"/>
        <property type="gene ID" value="ENSCING00000022267.1"/>
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    <dbReference type="InParanoid" id="H2XUA8"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
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    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
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        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003578172" description="Ig-like domain-containing protein" evidence="2">
        <location>
            <begin position="20"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="19"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="97"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="97"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000033242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="180" mass="19471" checksum="BEF4E5C305BF1E44" modified="2012-04-18" version="1">MTIMYFIIVTSAIIICVQANLNVTSGFDSPMTFDPDSNFTLRCRFRSDSDVTISWLRDGDVIAVGLNNGSETDEATGVGGARIGGDFTPLSIYKYTPSTSTETKTSRSVGRSNDDDTRHNRVSSVIFREDGDQVGELRVVGSNLEDGGIITCSASNARGERVEHAGRIVIKGPMSIRQLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>H2XUN8</accession>
    <name>H2XUN8_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">wild type</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001864">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XUN8"/>
    <dbReference type="STRING" id="7719.ENSCINP00000033372"/>
    <dbReference type="Ensembl" id="ENSCINT00000032241.1">
        <property type="protein sequence ID" value="ENSCINP00000033372.1"/>
        <property type="gene ID" value="ENSCING00000022067.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164512"/>
    <dbReference type="HOGENOM" id="CLU_085098_1_2_1"/>
    <dbReference type="InParanoid" id="H2XUN8"/>
    <dbReference type="OMA" id="IDCGFCA"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000062">
        <property type="entry name" value="Terribly reduced optic lobes, isoform AI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051221">
        <property type="entry name" value="LDLR-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22722:SF5">
        <property type="entry name" value="LOW-DENSITY LIPOPROTEIN RECEPTOR-RELATED PROTEIN 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22722">
        <property type="entry name" value="LOW-DENSITY LIPOPROTEIN RECEPTOR-RELATED PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00261">
        <property type="entry name" value="LDLRECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003577495" evidence="11">
        <location>
            <begin position="26"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="182"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="53"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="73"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="80"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="92"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="120"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="132"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="152"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="159"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000033372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="203" mass="22032" checksum="88D0FCCAB868B619" modified="2012-04-18" version="1">MNNSYTPKAWIIFCLAIITIVCVTSMPECKCKRKQFACSVSNKCTCIPVAWKCDGDTDCGDASDEMDCVIPTCRPSSFQCDNGKCIMAEWKCDGENDCGDDSDEVSCPIQPCDESTEKTCGSGDCVPKRWWCDGDEDCSDGSDETDCNRRTCGGTEYACTSGECVSSAFNCDGDFDCRDGSDEECEEPADPDVIPSNSNFTAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>H2XW81</accession>
    <name>H2XW81_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily. MetX family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="H2XW81"/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
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                <person name="Branno M."/>
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                <person name="Francino P."/>
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                <person name="Hino K."/>
                <person name="Imai K.S."/>
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                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
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                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
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                <person name="Inaba K."/>
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            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">wild type</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Chapman J."/>
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                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
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                <person name="Yoshizaki F."/>
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                <person name="Zhang C."/>
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                <person name="Richardson P."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
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                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">wild type</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <source>
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        <source>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
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                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
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                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <source>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
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                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
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                <person name="Huang W."/>
                <person name="Kawashima T."/>
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                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
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                <person name="Branno M."/>
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                <person name="Hayashi H."/>
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                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
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            <authorList>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
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                <person name="Medina M."/>
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                <person name="Miura S."/>
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                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
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                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
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            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
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    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
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                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
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                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
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                <person name="Lindquist E."/>
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        <source>
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        </source>
    </evidence>
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    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="H2Y2L4"/>
    <dbReference type="Ensembl" id="ENSCINT00000033709.1">
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        <property type="gene ID" value="ENSCING00000021503.1"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
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    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="161"/>
            <end position="184"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000036149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="228" mass="26787" checksum="47FC40FB7D2F0B15" modified="2012-04-18" version="1">KHKRQGGRKTRQNRKSGERRRSKSRGLRRRTKKKKNRSRNDDVFSEVKNIQNLWVNQAEERFDDDSYFRYYPGAFLDSVDSRSHIRIVNRLPTPAYDVTERNNLDSIFKFNWTPFDGSEENYLSTRQRYRPYTGYDVMNIRSDNWSDDANSGRRSTTQNNALVMTSLSVLLVSIIVSFLTDVIWMDYDVTTANKHKSFPPELSGRICDNRILQSRSKQIISPVADVNF</sequence>
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    <accession>H2Y2L7</accession>
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        <recommendedName>
            <fullName evidence="5">Protein cramped-like</fullName>
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            <fullName evidence="7">Cramped chromatin regulator homolog 1</fullName>
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        <alternativeName>
            <fullName evidence="6">Hematological and neurological expressed 1-like protein</fullName>
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    <organism evidence="10 11">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">wild type</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the cramped family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001262">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2Y2L7"/>
    <dbReference type="STRING" id="7719.ENSCINP00000036152"/>
    <dbReference type="Ensembl" id="ENSCINT00000036145.1">
        <property type="protein sequence ID" value="ENSCINP00000036152.1"/>
        <property type="gene ID" value="ENSCING00000023400.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000003337"/>
    <dbReference type="HOGENOM" id="CLU_1340196_0_0_1"/>
    <dbReference type="InParanoid" id="H2Y2L7"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000439">
        <property type="entry name" value="Cramped chromatin regulator homolog 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055315">
        <property type="entry name" value="Cramped-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21677">
        <property type="entry name" value="CRAMPED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21677:SF1">
        <property type="entry name" value="PROTEIN CRAMPED-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SANT" evidence="9">
        <location>
            <begin position="112"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
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        </location>
    </feature>
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        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00076971"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00081828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="205" mass="22825" checksum="49B63B4ED47D6F53" modified="2012-04-18" version="1">MDTDVKDKSDESSQSDAPNIGDPVEGRVWRRQSLTKPSKKSANGPVLRATVKPVKRFRPDSSTAPGPTPDATPLKVDASISCSNDDKVNSENGKQQRTEALINTPTSMKKKRVREQWSVEDKNHFFDAVAIHGKDFEAIQKAIAQKHRKKGGESVVKTKDQVRHLYYRTWTTVSKLLDGEVSSISSASNKTNQELFAVINYGELR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>H2Y2U6</accession>
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    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
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            <taxon>Metazoa</taxon>
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            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
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                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">wild type</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="EAAA01000775">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2Y3B3"/>
    <dbReference type="STRING" id="7719.ENSCINP00000036398"/>
    <dbReference type="Ensembl" id="ENSCINT00000032073.1">
        <property type="protein sequence ID" value="ENSCINP00000036398.1"/>
        <property type="gene ID" value="ENSCING00000018221.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000168216"/>
    <dbReference type="HOGENOM" id="CLU_1264071_0_0_1"/>
    <dbReference type="InParanoid" id="H2Y3B3"/>
    <dbReference type="OMA" id="ISSWFAD"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005216">
        <property type="term" value="F:monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050927">
        <property type="entry name" value="TRPM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13800:SF12">
        <property type="entry name" value="TRANSIENT RECEPTOR POTENTIAL CATION CHANNEL SUBFAMILY M MEMBER-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13800">
        <property type="entry name" value="TRANSIENT RECEPTOR POTENTIAL CATION CHANNEL, SUBFAMILY M, MEMBER 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="47"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="134"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="157"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="198"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="7">
        <location>
            <begin position="60"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000036398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="219" mass="25402" checksum="B15EA679F8A78DF4" modified="2012-04-18" version="1">FREIPDQSTSENDDLESVNSEVPNNELLKKDNKGKTKSTWRRKLVQFYRAPVVTFWLHVLSYLVYLLLFAYVILFNFYPQPATDVSLSNISVQEFVLWAWVCVLVVDEVYEVLQRDSKHIMGKISSWFADPWNKLDVVAIALFFIGIGLRFQTEVEASRTLLAISFMVYCWRILNIFLVHEALGPKLIMVGKMIKDMLFFLFILIIFLVSYGVASQAIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2026-01-28" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>Q8T885</accession>
    <accession>A0A1W2VNK3</accession>
    <name>Q8T885_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA-directed RNA polymerase III subunit RPC9</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">DNA-directed RNA polymerase III subunit rpc9</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">Ci-CGRP-RCP</name>
        <name evidence="16" type="synonym">cgrp-rcp</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2002-03" db="EMBL/GenBank/DDBJ databases">
            <title>Molecular cloning of ascidian CGRP-RCP-like protein.</title>
            <authorList>
                <person name="Inaba K."/>
                <person name="Kho K."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Testis</tissue>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">wild type</strain>
        </source>
    </reference>
    <reference evidence="16" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Accessory protein for the calcitonin gene-related peptide (CGRP) receptor. It modulates CGRP responsiveness in a variety of tissues.</text>
    </comment>
    <comment type="function">
        <text evidence="12">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III (Pol III) which synthesizes small non-coding RNAs including 5S rRNA, snRNAs, tRNAs and miRNAs from at least 500 distinct genomic loci. With POLR3H/RPC8 forms a mobile stalk that protrudes from Pol III core and functions primarily in transcription initiation. Pol III plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF-kappa-B through the RIG-I pathway.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of the RNA polymerase III complex consisting of 17 subunits: a ten-subunit horseshoe-shaped catalytic core composed of POLR3A/RPC1, POLR3B/RPC2, POLR1C/RPAC1, POLR1D/RPAC2, POLR3K/RPC10, POLR2E/RPABC1, POLR2F/RPABC2, POLR2H/RPABC3, POLR2K/RPABC4 and POLR2L/RPABC5; a mobile stalk composed of two subunits POLR3H/RPC8 and CRCP/RPC9, protruding from the core and functioning primarily in transcription initiation; and additional subunits homologous to general transcription factors of the RNA polymerase II machinery, POLR3C/RPC3-POLR3F/RPC6-POLR3G/RPC7 heterotrimer required for transcription initiation and POLR3D/RPC4-POLR3E/RPC5 heterodimer involved in both transcription initiation and termination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the eukaryotic RPC9 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001751">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AB081313">
        <property type="protein sequence ID" value="BAB85994.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001027775.1">
        <property type="nucleotide sequence ID" value="NM_001032603.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8T885"/>
    <dbReference type="STRING" id="7719.ENSCINP00000007376"/>
    <dbReference type="Ensembl" id="ENSCINT00000007376.3">
        <property type="protein sequence ID" value="ENSCINP00000007376.3"/>
        <property type="gene ID" value="ENSCING00000003588.3"/>
    </dbReference>
    <dbReference type="GeneID" id="445803"/>
    <dbReference type="KEGG" id="cin:445803"/>
    <dbReference type="CTD" id="445803"/>
    <dbReference type="GeneTree" id="ENSGT00390000014189"/>
    <dbReference type="HOGENOM" id="CLU_092529_5_0_1"/>
    <dbReference type="OMA" id="NTECKNQ"/>
    <dbReference type="OrthoDB" id="1746530at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006384">
        <property type="term" value="P:transcription initiation at RNA polymerase III promoter"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.40:FF:000002">
        <property type="entry name" value="DNA-directed RNA polymerase III subunit RPC9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010997">
        <property type="entry name" value="HRDC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006590">
        <property type="entry name" value="RNA_pol_Rpb4/RPC9_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005574">
        <property type="entry name" value="Rpb4/RPC9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038324">
        <property type="entry name" value="Rpb4/RPC9_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038846">
        <property type="entry name" value="RPC9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15561">
        <property type="entry name" value="CALCITONIN GENE-RELATED PEPTIDE-RECEPTOR COMPONENT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15561:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03874">
        <property type="entry name" value="RNA_pol_Rpb4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00657">
        <property type="entry name" value="RPOL4c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47819">
        <property type="entry name" value="HRDC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="RNA polymerase Rpb4/RPC9 core" evidence="14">
        <location>
            <begin position="1"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006898"/>
        </source>
    </evidence>
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        <source>
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        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00043924"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00044007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045808"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00073026"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="BAB85994.1"/>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000007376.3"/>
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    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    </evidence>
    <sequence length="124" mass="14079" checksum="583E271B6497FDEF" modified="2002-06-01" version="1">MEVLNILNEYANEQKLSKNKNSRNNQNLSTITYETIKYLSETPAGLQNQACIETTLKALLPFGLTKAEKLQLVNLRPTTAVEISLIVEESEERITEEQIDEIIEIVSRLPAPEEEQTAENMEET</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-06-07" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1W5BQ23</accession>
    <accession>F6Y0R8</accession>
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        <submittedName>
            <fullName evidence="3">Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein</fullName>
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        <name evidence="3" type="primary">LOC100181766</name>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
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    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
        </source>
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    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome 2"/>
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        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
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</entry>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="F6PJS1"/>
    <dbReference type="Ensembl" id="ENSCINT00000028968.2">
        <property type="protein sequence ID" value="ENSCINP00000028722.2"/>
        <property type="gene ID" value="ENSCING00000016655.2"/>
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    <dbReference type="HOGENOM" id="CLU_1187310_0_0_1"/>
    <dbReference type="InParanoid" id="F6PJS1"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003344300" evidence="1">
        <location>
            <begin position="25"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000028722.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="234" mass="26481" checksum="959D257AD7293507" modified="2012-04-18" version="2">MKTAAFSVVTCVFILFLFCNDVIGQGFPNFQKGQYLPRYPSGIPNRPQAPFLAPKQDTIADMAETFWKQNNCVYPPKEFYQMFRSACDVIITEQTFYAGICHEFFNLSQPLCGLDQATDDEIQTMTTIQDLQSNIGDDEICDTLSNMRRSEPDMFLHPTGRDSWFEPAYNLLFSSDTCQSVCSLDNVHPICFGLLLNFDYVMSTRQRAADDVAEDNVTNDPIGDVMNASDDVMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>F6PNS6</accession>
    <name>F6PNS6_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">S-adenosylmethionine synthase</fullName>
            <ecNumber evidence="11">2.5.1.6</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">wild type</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the formation of S-adenosylmethionine from methionine and ATP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>L-methionine + ATP + H2O = S-adenosyl-L-methionine + phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21080"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="2.5.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
        <text evidence="11">Binds 1 potassium ion per subunit. The potassium ion interacts primarily with the substrate.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="11">Binds 2 magnesium ions per subunit. The magnesium ions interact primarily with the substrate.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 11">Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the AdoMet synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.6" evidence="11"/>
    <dbReference type="EMBL" id="EAAA01000929">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6PNS6"/>
    <dbReference type="FunCoup" id="F6PNS6">
        <property type="interactions" value="23"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000016717"/>
    <dbReference type="Ensembl" id="ENSCINT00000016717.3">
        <property type="protein sequence ID" value="ENSCINP00000016717.3"/>
        <property type="gene ID" value="ENSCING00000008174.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183185"/>
    <dbReference type="HOGENOM" id="CLU_041802_0_1_1"/>
    <dbReference type="InParanoid" id="F6PNS6"/>
    <dbReference type="OMA" id="ASYMARY"/>
    <dbReference type="UniPathway" id="UPA00315">
        <property type="reaction ID" value="UER00080"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004478">
        <property type="term" value="F:methionine adenosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006556">
        <property type="term" value="P:S-adenosylmethionine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18079">
        <property type="entry name" value="S-AdoMet_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.10:FF:000001">
        <property type="entry name" value="S-adenosylmethionine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.10:FF:000003">
        <property type="entry name" value="S-adenosylmethionine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.10:FF:000004">
        <property type="entry name" value="S-adenosylmethionine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00086">
        <property type="entry name" value="S_AdoMet_synth1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022631">
        <property type="entry name" value="ADOMET_SYNTHASE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022630">
        <property type="entry name" value="S-AdoMet_synt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022629">
        <property type="entry name" value="S-AdoMet_synt_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022628">
        <property type="entry name" value="S-AdoMet_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002133">
        <property type="entry name" value="S-AdoMet_synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022636">
        <property type="entry name" value="S-AdoMet_synthetase_sfam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01034">
        <property type="entry name" value="metK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11964">
        <property type="entry name" value="S-ADENOSYLMETHIONINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02773">
        <property type="entry name" value="S-AdoMet_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02772">
        <property type="entry name" value="S-AdoMet_synt_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00438">
        <property type="entry name" value="S-AdoMet_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000497">
        <property type="entry name" value="MAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55973">
        <property type="entry name" value="S-adenosylmethionine synthetase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00376">
        <property type="entry name" value="ADOMET_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00377">
        <property type="entry name" value="ADOMET_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 11" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="9 11" id="KW-0630">Potassium</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="S-adenosylmethionine synthetase N-terminal" evidence="13">
        <location>
            <begin position="21"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="S-adenosylmethionine synthetase central" evidence="14">
        <location>
            <begin position="132"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="S-adenosylmethionine synthetase C-terminal" evidence="15">
        <location>
            <begin position="256"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000016717.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="400" mass="44180" checksum="96A9997C2EE4A474" modified="2012-04-18" version="2">MPETMISQNGFNGDGHEEETFLFTSESVGEGHPDKLCDQVSDAILDAHLAIDPNAKVACETFAKTGMVLVGGEITSKAVIDYQKVIRDTIKHIGYDDSSKGFDCKTCNVLLAVEQQSPEIADAVHIGKKEEDIGAGDQGLMFGYATDETEECMPLTVTLAHRLNEKLSELRRDGTLAWLRPDSKTQVTIEHRLEHGAVVPIRVHTIVISTQHDEQISLEKMRKEILEKIIKAVVPSQYLDENTIYHIQPSGKFITGGPMGDAGLTGRKIIVDTYGGWGAHGGGAFSGKDFSKVDRSAAYAARWVAKSLIKAGLCRRVLVQVSYAIGISEPLSVYVSSYGTSKLHDKELLQVINDNFDLRPGIIVRELNLRRPIYHETACYGHFGRKQFSWETPKTLVIRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>F6Q2A0</accession>
    <accession>A0A1W2W9R3</accession>
    <name>F6Q2A0_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized LOC100186076</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC100186076</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">wild type</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="protein sequence ID" value="ENSCINP00000001358.3"/>
        <property type="gene ID" value="ENSCING00000013176.2"/>
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    <dbReference type="KEGG" id="cin:100186076"/>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    </evidence>
    <sequence length="307" mass="33650" checksum="DFC82FFF8AA57E86" modified="2011-07-27" version="1">MAAKMIFFVVAAAIAYALSLIFAYLNTAKVKGLYYSDNGEVDAKYYQEASPIGTAFGIVWPIIYLWNLAGIIYMLVSLWLPEIKSPLKTNPPILNNVFFAGYIMSFSTTIGWLFAFDREILGLALALLILAVASVYIAIGSCCKALAHNGASLTRLPDQKKSVEWTVRIVIQNGLSTFATWLTVATLLNLADVILYQDSFGITPAVQRGLISIQNGSTVVLIILLAIIIAWFILDNFIWDKFTRFIFTPYAVLILALSGIIAKLRPVTQSNPQNLIIASVELGIVVIALLAKISLVTYRCVTRGTAV</sequence>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
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        </lineage>
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    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
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                <person name="Inaba K."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">wild type</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
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    <comment type="similarity">
        <text evidence="2 7">Belongs to the actin family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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    <dbReference type="Ensembl" id="ENSCINT00000018112.3">
        <property type="protein sequence ID" value="ENSCINP00000018112.3"/>
        <property type="gene ID" value="ENSCING00000008902.3"/>
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    <dbReference type="GeneTree" id="ENSGT00950000182960"/>
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    <dbReference type="InParanoid" id="F6QBJ1"/>
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    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0051286">
        <property type="term" value="C:cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030427">
        <property type="term" value="C:site of polarized growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd10224">
        <property type="entry name" value="ASKHA_NBD_actin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Actin, muscle"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Putative actin-related protein 5"/>
        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="entry name" value="Actin"/>
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        <property type="entry name" value="Actin/actin-like_CS"/>
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        <property type="entry name" value="Actin_CS"/>
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        <property type="entry name" value="ATPase_NBD"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Actin"/>
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        <property type="entry name" value="ACTINS_ACT_LIKE"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006752"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000018112.3"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="378" mass="42004" checksum="6F0185F687D00223" modified="2012-04-18" version="2">MLDDDIAALVIDNGSGMCKAGFAGDDAPRAVFPSLVGRPKHQGVMVGMGQKDSYVGDEAQSKRGILSLKYPIEHGVVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNAPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRQDLAGRDLTNYLMKILTERGYSFTTTVTAEREIVRDIKEKLCYVALDYDQELATAATSSSLEKSYELPDGQVITIGNERFRCPEAMFQPTMLGMESTGIHETTYNSIMKCDVDIRKDLYANTVLSGGSTMYPGIGDRMQKEITSLAPSTMRIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>F6QBL6</accession>
    <name>F6QBL6_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Integrin beta</fullName>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
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        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
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                <person name="DeSantis R."/>
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                <person name="Francino P."/>
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                <person name="Hayashi H."/>
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                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
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                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">wild type</strain>
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    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    <comment type="similarity">
        <text evidence="2 15">Belongs to the integrin beta chain family.</text>
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</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
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            <taxon>Phlebobranchia</taxon>
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    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">wild type</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the CDC50/LEM3 family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01000500">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6QH95"/>
    <dbReference type="FunCoup" id="F6QH95">
        <property type="interactions" value="647"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000016913"/>
    <dbReference type="Ensembl" id="ENSCINT00000016913.3">
        <property type="protein sequence ID" value="ENSCINP00000016913.3"/>
        <property type="gene ID" value="ENSCING00000008282.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000004660"/>
    <dbReference type="HOGENOM" id="CLU_025025_1_0_1"/>
    <dbReference type="InParanoid" id="F6QH95"/>
    <dbReference type="OMA" id="AWKPLYT"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015247">
        <property type="term" value="F:aminophospholipid flippase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005045">
        <property type="entry name" value="CDC50/LEM3_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10926:SF0">
        <property type="entry name" value="CDC50, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10926">
        <property type="entry name" value="CELL CYCLE CONTROL PROTEIN 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03381">
        <property type="entry name" value="CDC50"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DUF284_TM_euk"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="32"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="313"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRNR" id="PIRNR015840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000016913.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="360" mass="40631" checksum="6169938BF76664D3" modified="2012-04-18" version="2">VMEKEKRHIPDNTAFKQQSMPAWSPIISAKSALPVFFIISLAFVPIGVVLLVTSQSVVEHQHDYTDCVSVENPGVPCGLLRMNQSQMTQPCTCILNITLETSMEGNVYMYYGLTNFFQNHRRYVKSRDDDQLIGQHKSSAQVYSCAPYHLAVENGTLRTIAPCGAIANSLFNDTFTLSQGDIEVPFLRTGIAWPTDKSAKFNNPPPTTDLEEAFKIYAKPPNWQKPVTFLDRNNTDNNGYENEAFIVWMRPAAFPHFRKPYGRLNRLGSEYNNGLPSGQYKVTINYNFPVTSFGGRKRIILSTTTWMGGKNNFLGIAYITFGTICFFGGLVLTAVHLHARNTREFYLHKCLFVAQQNCHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>F6QKD8</accession>
    <name>F6QKD8_CIOIN</name>
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        <recommendedName>
            <fullName evidence="5">BACK domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="F6QKD8"/>
    <dbReference type="STRING" id="7719.ENSCINP00000025258"/>
    <dbReference type="Ensembl" id="ENSCINT00000025504.2">
        <property type="protein sequence ID" value="ENSCINP00000025258.2"/>
        <property type="gene ID" value="ENSCING00000002846.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157891"/>
    <dbReference type="HOGENOM" id="CLU_004253_3_1_1"/>
    <dbReference type="InParanoid" id="F6QKD8"/>
    <dbReference type="OMA" id="EPQVHNA"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632:SF3">
        <property type="entry name" value="KELCH-LIKE PROTEIN 32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632">
        <property type="entry name" value="LD33804P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="4"/>
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        <property type="entry name" value="KELCHREPEAT"/>
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        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
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    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000025258.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="235" mass="26025" checksum="ADD2C88AAF854FC9" modified="2012-04-18" version="2">MPILLEHVRLPLMPSDYLVERVDQESLVKNNDLCKDFLIEAMKYHLLSPEHRAMMKISRTRPRTPKGMPKSMLVVGGQAPKAIRSVECYDFKEDRWRQVSEMNSRRCRAGVSVFQGCVWAVGGFNGSLRVRTVDKYDPMTDCWTAGPSMEARRSTLGAAVLQNMLYAVGGFDGSSGLNTAEVYDPKTNEWRSIAPMNTRRSSVGVAVLGGLLYAVGGYDGVSRTLLGVGSNVSQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>F6QKI0</accession>
    <name>F6QKI0_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
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                <person name="Doyle S."/>
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                <person name="Kobayashi K."/>
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                <person name="Makabe K.W."/>
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                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
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                <person name="Nishizaka S."/>
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                <person name="Tassy O."/>
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                <person name="Doggett N."/>
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                <person name="Richardson P."/>
                <person name="Lucas S."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
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                <person name="Chin-Bow S."/>
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                <person name="Oishi K."/>
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                <person name="Sasaki A."/>
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                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
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        <scope>IDENTIFICATION</scope>
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                <consortium name="Ensembl"/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="158" mass="16916" checksum="C1219A1D4DCA7F25" modified="2012-04-18" version="2">MFNWFVNSFVYYGISLNAGALAGDIFVNNTLNGVMEIGSYVLCILLMDRIGRRILLSGMMFLSGIGLIISLVVNEYKGSNQGLETLSLVFAFAAKIGISGSFAIIYNLTSELYPTVVRSNGVGAGSLMARVGGIIAPFLIALQDDVTWLPNAIFGIFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>F6R4R6</accession>
    <name>F6R4R6_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">HRDC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">wild type</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the exosome component 10/RRP6 family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001160">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6R4R6"/>
    <dbReference type="STRING" id="7719.ENSCINP00000004852"/>
    <dbReference type="Ensembl" id="ENSCINT00000004852.3">
        <property type="protein sequence ID" value="ENSCINP00000004852.3"/>
        <property type="gene ID" value="ENSCING00000002381.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015408"/>
    <dbReference type="HOGENOM" id="CLU_042387_2_0_1"/>
    <dbReference type="InParanoid" id="F6R4R6"/>
    <dbReference type="OMA" id="LIPCESS"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003727">
        <property type="term" value="F:single-stranded RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000467">
        <property type="term" value="P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071044">
        <property type="term" value="P:histone mRNA catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071040">
        <property type="term" value="P:nuclear polyadenylation-dependent antisense transcript catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071039">
        <property type="term" value="P:nuclear polyadenylation-dependent CUT catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071035">
        <property type="term" value="P:nuclear polyadenylation-dependent rRNA catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071036">
        <property type="term" value="P:nuclear polyadenylation-dependent snoRNA catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071037">
        <property type="term" value="P:nuclear polyadenylation-dependent snRNA catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071051">
        <property type="term" value="P:poly(A)-dependent snoRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071038">
        <property type="term" value="P:TRAMP-dependent tRNA surveillance pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06147">
        <property type="entry name" value="Rrp6p_like_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.80:FF:000001">
        <property type="entry name" value="Putative exosome component 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.80">
        <property type="entry name" value="HRDC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002562">
        <property type="entry name" value="3'-5'_exonuclease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010997">
        <property type="entry name" value="HRDC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002121">
        <property type="entry name" value="HRDC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044876">
        <property type="entry name" value="HRDC_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045092">
        <property type="entry name" value="Rrp6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049559">
        <property type="entry name" value="Rrp6p-like_exo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12124:SF47">
        <property type="entry name" value="EXOSOME COMPONENT 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12124">
        <property type="entry name" value="POLYMYOSITIS/SCLERODERMA AUTOANTIGEN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01612">
        <property type="entry name" value="DNA_pol_A_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00570">
        <property type="entry name" value="HRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00474">
        <property type="entry name" value="35EXOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00341">
        <property type="entry name" value="HRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47819">
        <property type="entry name" value="HRDC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50967">
        <property type="entry name" value="HRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HRDC" evidence="7">
        <location>
            <begin position="206"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00043957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000004852.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="292" mass="33539" checksum="16B45357D2E0009D" modified="2012-04-18" version="2">MDELKEASEIAVDLEAHSYRSYQGITCLMQLSTRTKDYIVDTIALRAELNILNQVFANPNIIKVFHGADSDIIWLQRDFGIYVVNLFDTGQAARALGLQRHSLDYLLTHYCNVQADKKYQLADWRIRPLPKEMLLYAQGDTHYLLYVYDMMRLDLVKTGDPGLLHKVIDKSRDICCLKYEKPITNDTSHLVLLEKHKRRGGKKDFRPQQIEALRLIFAWRDGLARQEDESCGYVLPNHMLLQIAEILPREAQGVLACCNPIPPLLRQHVLAVHQLVVEARNKVFKSDNGGLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>F6R9Y6</accession>
    <accession>A0A1W2WN60</accession>
    <name>F6R9Y6_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">L-proline trans-4-hydroxylase-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC100185132</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="EAAA01000069">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002131674.1">
        <property type="nucleotide sequence ID" value="XM_002131638.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6R9Y6"/>
    <dbReference type="STRING" id="7719.ENSCINP00000025181"/>
    <dbReference type="Ensembl" id="ENSCINT00000025427.2">
        <property type="protein sequence ID" value="ENSCINP00000025181.2"/>
        <property type="gene ID" value="ENSCING00000013799.2"/>
    </dbReference>
    <dbReference type="GeneID" id="100185132"/>
    <dbReference type="KEGG" id="cin:100185132"/>
    <dbReference type="GeneTree" id="ENSGT00390000006287"/>
    <dbReference type="HOGENOM" id="CLU_048953_5_1_1"/>
    <dbReference type="InParanoid" id="F6R9Y6"/>
    <dbReference type="OMA" id="RIDHEMV"/>
    <dbReference type="OrthoDB" id="445007at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008775">
        <property type="entry name" value="Phytyl_CoA_dOase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20883">
        <property type="entry name" value="PHYTANOYL-COA DIOXYGENASE DOMAIN CONTAINING 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20883:SF51">
        <property type="entry name" value="PHYTANOYL-COA HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05721">
        <property type="entry name" value="PhyH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000025181.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="287" mass="32825" checksum="28619516FB49837B" modified="2012-04-18" version="2">MYKFVEGQPLIVTEKMKEDFWRDGYIIVKQLLTKGEIQKVVGSLEAPDSAVMAESYEQDDGFGRNVRMVLWNHPGNDVTGMVNRCEKMVNTCEKLLGDDVYHYHSKFVMKEPHTGGAFQWHQDYGYWYLNGVLFPDMISVQIGVDRMDKENGCLQVLRGSHRMGRVEHGRIGQQAGADLERVAEAEKVLDKVSVELDQGDALFFHCNLLHTSSANNSSRRRWAMICCYNSVNNDPVKKHHHASYTPLHKVSNSAIMDCKNLNDLSGKWFVNPNKTGREYLPTEPRKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>F6RM19</accession>
    <name>F6RM19_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mitochondrial mRNA-processing protein COX24 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="F6RM19"/>
    <dbReference type="Ensembl" id="ENSCINT00000024172.2">
        <property type="protein sequence ID" value="ENSCINP00000023926.2"/>
        <property type="gene ID" value="ENSCING00000012926.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000009859"/>
    <dbReference type="HOGENOM" id="CLU_1510080_0_0_1"/>
    <dbReference type="InParanoid" id="F6RM19"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000023926.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="178" mass="20863" checksum="A137FCEF82F4B562" modified="2012-04-18" version="2">MLLGFRNVLSRVSPIILKQTSQNCTQVNKSLPYLFGENKLSPLISKPQETNHVILSRVDSVGFIPNKFDELIIASSIMSRISCPSNQPLPTHNKYNIDSTEHGKVINEGVDVLDVFDEPNTSNQTPLYAHKSDIRWRRRRMRRHRLLKWRKKYKHLVKLRLKQKLERRKVLLDNDLVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>F6S0X0</accession>
    <name>F6S0X0_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ATP-dependent RNA helicase</fullName>
            <ecNumber evidence="7">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">wild type</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">RNA helicase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="domain">
        <text evidence="7">The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the DEAD box helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="7"/>
    <dbReference type="EMBL" id="EAAA01002177">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6S0X0"/>
    <dbReference type="FunCoup" id="F6S0X0">
        <property type="interactions" value="195"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000018842"/>
    <dbReference type="Ensembl" id="ENSCINT00000018842.3">
        <property type="protein sequence ID" value="ENSCINP00000018842.3"/>
        <property type="gene ID" value="ENSCING00000009272.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00550000075041"/>
    <dbReference type="HOGENOM" id="CLU_003041_26_2_1"/>
    <dbReference type="InParanoid" id="F6S0X0"/>
    <dbReference type="OMA" id="AVHIKAD"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025313">
        <property type="entry name" value="SPB4-like_CTE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24031">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13959">
        <property type="entry name" value="CTE_SPB4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01178">
        <property type="entry name" value="DUF4217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
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                <person name="DeSantis R."/>
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                <person name="Makabe K.W."/>
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                <person name="Mochizuki Y."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Huang W."/>
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                <person name="Lemaire P."/>
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                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4">wild type</strain>
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        <scope>IDENTIFICATION</scope>
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                <consortium name="Ensembl"/>
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            <end position="338"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="602"/>
            <end position="639"/>
        </location>
    </feature>
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        <location>
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            <end position="728"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="81"/>
            <end position="91"/>
        </location>
    </feature>
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        <location>
            <begin position="128"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="186"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="303"/>
            <end position="316"/>
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    </feature>
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        <location>
            <begin position="322"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="602"/>
            <end position="612"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="618"/>
            <end position="639"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50106"/>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000002382.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="729" mass="81894" checksum="10A7EC00EF4DA881" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>F6SY72</accession>
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        <recommendedName>
            <fullName evidence="9">Fork-head domain-containing protein</fullName>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
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            <taxon>Phlebobranchia</taxon>
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        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">wild type</strain>
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    <reference evidence="10" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="10" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <location evidence="1 7">Nucleus</location>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="evidence" value="ECO:0000318"/>
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        <property type="match status" value="1"/>
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        <location>
            <begin position="38"/>
            <end position="116"/>
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    </evidence>
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            <dbReference type="Proteomes" id="UP000008144"/>
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</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>F6SZ23</accession>
    <name>F6SZ23_CIOIN</name>
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            <fullName evidence="2">receptor protein-tyrosine kinase</fullName>
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    <organism evidence="14 15">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9 16">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.10.1" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="F6SZ23"/>
    <dbReference type="STRING" id="7719.ENSCINP00000002343"/>
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        <property type="protein sequence ID" value="ENSCINP00000002343.3"/>
        <property type="gene ID" value="ENSCING00000001202.3"/>
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    <dbReference type="HOGENOM" id="CLU_015827_0_0_1"/>
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        <property type="term" value="C:basal plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="GO_Central"/>
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        <property type="term" value="C:receptor complex"/>
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    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="F:transmembrane receptor protein tyrosine kinase activity"/>
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        <property type="project" value="GO_Central"/>
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        <property type="term" value="P:epidermal growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="P:negative regulation of apoptotic process"/>
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        <property type="project" value="GO_Central"/>
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        <property type="term" value="P:neuron differentiation"/>
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        <property type="term" value="P:positive regulation of epithelial cell proliferation"/>
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        <property type="entry name" value="PTKc_EGFR_like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Receptor protein-tyrosine kinase"/>
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        <property type="entry name" value="Receptor protein-tyrosine kinase"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
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        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
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        <property type="entry name" value="Egfr_JX_dom"/>
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        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
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    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
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    <dbReference type="InterPro" id="IPR050122">
        <property type="entry name" value="RTK"/>
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    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
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        <property type="entry name" value="EPIDERMAL GROWTH FACTOR RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416">
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        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
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        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
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        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
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    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
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        <location>
            <begin position="40"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="123"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="464"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="723"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="470"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="775"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00011902"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00051243"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
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        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000002343.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="880" mass="97936" checksum="B83BA98884B7F609" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>F6T0L9</accession>
    <name>F6T0L9_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    <dbReference type="FunCoup" id="F6T0L9">
        <property type="interactions" value="90"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000017835"/>
    <dbReference type="Ensembl" id="ENSCINT00000017835.3">
        <property type="protein sequence ID" value="ENSCINP00000017835.3"/>
        <property type="gene ID" value="ENSCING00000008753.3"/>
    </dbReference>
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    <dbReference type="HOGENOM" id="CLU_020460_0_0_1"/>
    <dbReference type="InParanoid" id="F6T0L9"/>
    <dbReference type="OMA" id="WSEFFEN"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000038">
        <property type="entry name" value="TBC1 domain family member 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000011">
        <property type="entry name" value="TBC1 domain family member 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957:SF337">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="2">
        <location>
            <begin position="51"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000017835.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="398" mass="47117" checksum="E486F5C9F9E064F0" modified="2012-04-18" version="2">MESNGTSAVSDLSHDTKQAGVNSYVEQWNDYFEGHENLIEYLKYKTYSHSLRRCHFRSVCWRIFLHCLPHNTSEWPAALVKSRAEYDVIKERNITIPNSEKQPSNIEVENPLSQNDDSTWLQYFRDKELRNDIERDVRRTFPEMDYFSKEDVRHAMENILFCYAREHTNLSYRQGMHELLAPILFVLHCDLQGAFHTQEMGELPLPTFFRPIIQTVFQQKYLENDAYTMFCQLMRSTNPWYSINEIEPELPLECAKNNDPIPTVPFQPSEENNAGPPLEITNKLNRIHNDLLATYDHELYYHISRLEIIPQVYGLRWVRLLFGREFDLQDLLVLWDTMFADSSALDLVDYIFVALMVNIREQLLAADYCTCMRILMKYPPIEDIFDIVHLAIYYRDPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F6T0Z8</accession>
    <name>F6T0Z8_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01002034">
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        <property type="molecule type" value="Genomic_DNA"/>
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    <proteinExistence type="predicted"/>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="35"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="130" mass="14378" checksum="7C33024D2F3D29BC" modified="2012-04-18" version="2">MSTPRSNRHSTDILKLPSLCTRSNSAPVNRRPSNDGATSSDADSGFQASRATEDMDESSNFKPRDGAVEQVNSTRVDPCCKISLDSATSGEKIAKSRKERKDEIDITTLAKIEEFENFAREKLSRQHSKT</sequence>
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    <reference evidence="5" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
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                <person name="Francino P."/>
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                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="F6T646"/>
    <dbReference type="STRING" id="7719.ENSCINP00000015740"/>
    <dbReference type="Ensembl" id="ENSCINT00000015740.3">
        <property type="protein sequence ID" value="ENSCINP00000015740.3"/>
        <property type="gene ID" value="ENSCING00000007683.3"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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        <location>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
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    </evidence>
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    </evidence>
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        <source>
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    <sequence length="171" mass="18562" checksum="52D688FD12AE8EC9" modified="2012-04-18" version="2">MLSWFNYTEAMWIGLQENGEGGWSWTDGRPVSFTYWNEGEPNDAGEDGEDCVEMYGNSGKWNDIPCSNQHGYMCRVPKILGTDANGNNIHFTTTETNKPIKANTTGIVVGVVIGVLLAIIVLLSLAYYCSRNSSPSVSKLAEDASDFSSGIVNKVYSLTGSTSTPKEGDEV</sequence>
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    <accession>F6TNK4</accession>
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        <recommendedName>
            <fullName evidence="3">BTB domain-containing protein</fullName>
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    <reference evidence="5" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
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                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
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                <person name="Doyle S."/>
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                <person name="Hayashi H."/>
                <person name="Hino K."/>
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                <person name="Kobayashi M."/>
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                <person name="Makabe K.W."/>
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                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
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                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
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                <person name="Doggett N."/>
                <person name="Glavina T."/>
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                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
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    <feature type="domain" description="BTB" evidence="3">
        <location>
            <begin position="33"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
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    </evidence>
    <sequence length="418" mass="48126" checksum="CA08AA2E74DB716B" modified="2012-04-18" version="2">MATNDEHVIQYKGDHEGRVLTKLNRLRKRKVFTDVTFKLEDGEVSAQKSILAGYSDYYEKMFTSGFKEGFNKDIEIKEMKASILDLLFTFIYLQVIDISDKTVCQLYEASDYLQLNDVKGYCVSFFNDALAVGNCLSFRSFAQRYQLLELVGKCDKFIVDNLELVSKEFQFMELSVNEAEALIALKQKQDSCQDSIFRTILNWIKHDFKQRQQFIEQLFQLIDVKKLSTAFLEEVVKESEFYHLYFPCSILQSPALTFYLLHFYNHKIISLTLKATFEFMIVGGRGTEKLVQIYDVVGKHLREIRFYPQSTLYERWASTSVKINNHVYTTGGHRSNVVECLNLNQVDGDWYKVASMNETRWGAASSVLNDQMCVAGGRDSGNNVLSSVELYNPVVNIWTNIASMQTERLLHALVSCNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>F6TT65</accession>
    <name>F6TT65_CIOIN</name>
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        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
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    </protein>
    <organism evidence="5 6">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
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            <taxon>Phlebobranchia</taxon>
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            <taxon>Ciona</taxon>
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    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">wild type</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="EAAA01001919">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6TT65"/>
    <dbReference type="STRING" id="7719.ENSCINP00000008858"/>
    <dbReference type="Ensembl" id="ENSCINT00000008858.3">
        <property type="protein sequence ID" value="ENSCINP00000008858.3"/>
        <property type="gene ID" value="ENSCING00000004287.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000016876"/>
    <dbReference type="HOGENOM" id="CLU_1274765_0_0_1"/>
    <dbReference type="InParanoid" id="F6TT65"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043024">
        <property type="term" value="F:ribosomal small subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037387">
        <property type="entry name" value="PTCD3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055063">
        <property type="entry name" value="Rib_mS39_PPR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16276">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS39"/>
        <property type="match status" value="1"/>
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    </dbReference>
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    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
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    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
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        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000008858.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="217" mass="24866" checksum="8B4744B7B84D5E84" modified="2012-04-18" version="2">MSMRIHRFFRFSTRKYHTSNLLSNKEASTNLVYDAKSIIEKQLQASVQVPKKKIWHEKAVLEALEGSTNLIHLPNHTLETPDYWPKGTLSKKTDTAALKSGKAAAEYIIQNHPEYFQDDWDATNVETWMKNFDEDLPALEDGAKSKDNVEKKEEKVFSDSVDSLNELLELKKVEKSGEMYLRLHKTSVEVPTSTTNQLLGLLAYTRCSTDELKSTKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>F6UFS0</accession>
    <name>F6UFS0_CIOIN</name>
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        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
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            <taxon>Tunicata</taxon>
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            <taxon>Phlebobranchia</taxon>
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    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">wild type</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="EAAA01002677">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">wild type</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Meinertzhagen I.A."/>
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                <person name="Putnam N."/>
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                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
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                <person name="Mochizuki Y."/>
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                <person name="Tassy O."/>
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    </reference>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
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                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
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                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">wild type</strain>
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    <reference evidence="9" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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                <person name="Chapman J."/>
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                <person name="De Tomaso A."/>
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                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
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                <person name="Hastings K.E."/>
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                <person name="Terry A."/>
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                <person name="Azumi K."/>
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                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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            <strain evidence="2">wild type</strain>
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                <consortium name="Ensembl"/>
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        </citation>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
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                <person name="Imai K.S."/>
                <person name="Inaba K."/>
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                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
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                <person name="Makabe K.W."/>
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                <person name="Matassi G."/>
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                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
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                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
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                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
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                <person name="Tokuoka M."/>
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                <person name="Zhang C."/>
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                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">wild type</strain>
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    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <source>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
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                <person name="Putnam N."/>
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                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
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        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
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        <property type="entry name" value="SH2_dom_sf"/>
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    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24418">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 13 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="2 12" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="4 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 14" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="SH3" evidence="16">
        <location>
            <begin position="7"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="15">
        <location>
            <begin position="76"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="192"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="219"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00051245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU362096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000007054.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="454" mass="51768" checksum="BA8155C63AA8EBD8" modified="2012-04-18" version="2">LLFQTIPNGTTCEGVFDFNTGNKGDLPFKKGDTLVVIQRTRDPNWYRAQNSKGESGMVPLNYITQHKEVKLTAMPWFHGKITRSDAEDLLTPRKDGLFLVRESTNYPGDYTMCVSFAGEVEHYRVIFKDNKVTIDEEEYFENLTKLVEHYERDADGLVCTLTQPLIKKGGHEYSVDTEEFRKSGWAINFTDLKLGTILGKGDFGDVYEGTYKDQKVAVKSVKDRSKAAQAFLAEASVMTQLHHKNLIKLLGVVIPQTMSNDPIYVVLEFMTKGAMLEYLRSRGRALVTSNDLWKFAKDIALGMEYLENKQLVHRDLAARNILISEECVAKVSDFGLARDASFNLQGGKFPVKWTAPEALKEGKFSNKSDVWSYGITLWEMWSFGRVPYPRVPLKDLLAKVQSGYRMDCPERCDKIVYQLMLKCWLKDVNERLSFKQIVHELDVIKPKLLPAINP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>F6XM64</accession>
    <name>F6XM64_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NOSIC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="F6XM64"/>
    <dbReference type="STRING" id="7719.ENSCINP00000005742"/>
    <dbReference type="Ensembl" id="ENSCINT00000005742.3">
        <property type="protein sequence ID" value="ENSCINP00000005742.3"/>
        <property type="gene ID" value="ENSCING00000002806.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00550000075069"/>
    <dbReference type="HOGENOM" id="CLU_026337_0_0_1"/>
    <dbReference type="InParanoid" id="F6XM64"/>
    <dbReference type="OMA" id="DPEYDQQ"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000244">
        <property type="term" value="P:spliceosomal tri-snRNP complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.4070:FF:000003">
        <property type="entry name" value="U4/U6 small nuclear ribonucleoprotein PRP31"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Nop_dom"/>
    </dbReference>
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        <property type="entry name" value="Nop_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012976">
        <property type="entry name" value="NOSIC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027105">
        <property type="entry name" value="Prp31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13904">
        <property type="entry name" value="PRE-MRNA SPLICING FACTOR PRP31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13904:SF0">
        <property type="entry name" value="U4_U6 SMALL NUCLEAR RIBONUCLEOPROTEIN PRP31"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Nop"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NOSIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89124">
        <property type="entry name" value="Nop domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NOSIC" evidence="1">
        <location>
            <begin position="96"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000005742.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="220" mass="24455" checksum="5651D3606B7B3BA1" modified="2012-04-18" version="2">MSLADELLADLEDAGIDEDAVVDDNGEGDGIDDIEDVGVSMDTGQDSIDPNSIHSVARLSSTDQFKRVVQGIEDYCNKPQSSVAAGIIESNPEYKLIVESNNLTMEIDNEVNIIHKFTRDYYSKRFPELDSLVPTPLEYIRTVKELGNDLEKYKNNEVLQQVMSNATIMVISVTASTTQGKLLSEEELNRVFEACTMAEELMSAKLKILTYVESRMAFIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>F6XT75</accession>
    <name>F6XT75_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HECT-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.26</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">wild type</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="3"/>
    <dbReference type="EMBL" id="EAAA01000623">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6XT75"/>
    <dbReference type="FunCoup" id="F6XT75">
        <property type="interactions" value="75"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000015931"/>
    <dbReference type="Ensembl" id="ENSCINT00000015931.3">
        <property type="protein sequence ID" value="ENSCINP00000015931.3"/>
        <property type="gene ID" value="ENSCING00000007771.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156723"/>
    <dbReference type="HOGENOM" id="CLU_014403_0_0_1"/>
    <dbReference type="InParanoid" id="F6XT75"/>
    <dbReference type="OMA" id="GHLCKDA"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00078">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2160.10:FF:000008">
        <property type="entry name" value="Apoptosis-resistant E3 ubiquitin protein ligase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050409">
        <property type="entry name" value="E3_ubiq-protein_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058738">
        <property type="entry name" value="PH-like_AREL1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254:SF340">
        <property type="entry name" value="APOPTOSIS-RESISTANT E3 UBIQUITIN PROTEIN LIGASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254">
        <property type="entry name" value="HECT DOMAIN UBIQUITIN-PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25916">
        <property type="entry name" value="AREL1_PH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="HECT" evidence="7">
        <location>
            <begin position="325"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="6">
        <location>
            <position position="635"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000015931.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="668" mass="75417" checksum="0652A037AC709E23" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>F6Y7Q5</accession>
    <name>F6Y7Q5_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tyrosine 3-monooxygenase</fullName>
            <ecNumber evidence="5">1.14.16.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Tyrosine 3-hydroxylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">th</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">wild type</strain>
        </source>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 16">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Perinuclear region</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.</text>
    </comment>
    <dbReference type="EC" id="1.14.16.2" evidence="5"/>
    <dbReference type="EMBL" id="EAAA01001566">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6Y7Q5"/>
    <dbReference type="STRING" id="7719.ENSCINP00000014787"/>
    <dbReference type="Ensembl" id="ENSCINT00000014787.3">
        <property type="protein sequence ID" value="ENSCINP00000014787.3"/>
        <property type="gene ID" value="ENSCING00000007206.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182885"/>
    <dbReference type="HOGENOM" id="CLU_023198_0_1_1"/>
    <dbReference type="InParanoid" id="F6Y7Q5"/>
    <dbReference type="OMA" id="QKYALTI"/>
    <dbReference type="UniPathway" id="UPA00747">
        <property type="reaction ID" value="UER00733"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043204">
        <property type="term" value="C:perikaryon"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004511">
        <property type="term" value="F:tyrosine 3-monooxygenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050890">
        <property type="term" value="P:cognition"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006585">
        <property type="term" value="P:dopamine biosynthetic process from tyrosine"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007617">
        <property type="term" value="P:mating behavior"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042427">
        <property type="term" value="P:serotonin biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001963">
        <property type="term" value="P:synaptic transmission, dopaminergic"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03345">
        <property type="entry name" value="eu_TyrOH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.800.10">
        <property type="entry name" value="Aromatic amino acid hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001273">
        <property type="entry name" value="ArAA_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018301">
        <property type="entry name" value="ArAA_hydroxylase_Fe/CU_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036951">
        <property type="entry name" value="ArAA_hydroxylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036329">
        <property type="entry name" value="Aro-AA_hydroxylase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019774">
        <property type="entry name" value="Aromatic-AA_hydroxylase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041903">
        <property type="entry name" value="Eu_TyrOH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019773">
        <property type="entry name" value="Tyrosine_3-monooxygenase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11473">
        <property type="entry name" value="AROMATIC AMINO ACID HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11473:SF15">
        <property type="entry name" value="TYROSINE 3-MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00351">
        <property type="entry name" value="Biopterin_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000336">
        <property type="entry name" value="TH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00372">
        <property type="entry name" value="FYWHYDRXLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56534">
        <property type="entry name" value="Aromatic aminoacid monoxygenases, catalytic and oligomerization domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00367">
        <property type="entry name" value="BH4_AAA_HYDROXYL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51410">
        <property type="entry name" value="BH4_AAA_HYDROXYL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0127">Catecholamine biosynthesis</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12 15" id="KW-0408">Iron</keyword>
    <keyword evidence="9 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="10" id="KW-0530">Neurotransmitter biosynthesis</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Biopterin-dependent aromatic amino acid hydroxylase family profile" evidence="18">
        <location>
            <begin position="105"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="10"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="284"/>
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        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
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        <location>
            <position position="289"/>
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        <ligand>
            <name>Fe cation</name>
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            <name>Fe cation</name>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
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            <dbReference type="ARBA" id="ARBA00004700"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00032379"/>
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        <source>
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        <source>
            <dbReference type="PIRSR" id="PIRSR601273-2"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS51410"/>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000014787.3"/>
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    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    </evidence>
    <sequence length="429" mass="48441" checksum="94A1357AA3107E64" modified="2012-04-18" version="2">MTSTSRRGSLIRDAENAEKENASSSSSKSGFWKKCEIVVTSAGNTMSQICEKLKNEDVIVSYIETRNGTLIKNVAGNSNTSFLITCIGNDNDITTGLKRLESIGCKATIVNGTERTAEWFPRHVTELELCRGTKTDYEPDKDSNHPGFNDPVYVERRNYISNIAHFYKHGTDIPTVDYTNEDRQTWSVVYKTLKRLHATHACKVYKDNFQRLEKECGYSPNKIPQLQTVSEFLKEQTGFQLQPAPGIITPRDFLASLAFKVFQCTQYIRHPASPMHSPEPDCCHELIGHIPMLLDPTFALYSQQIGLASLGVSDSDITKLATLYWFTVEFGLCKENNVLKAYGAGLMSSYGELQHALSDVPMHLPLQAEKTCLQPYEDSVYQPIYFVSESFDEAFNQVRAFSQHCTKRGFDITYNENDGYIQTVPYINL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>F6YGU8</accession>
    <name>F6YGU8_CIOIN</name>
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        <recommendedName>
            <fullName evidence="18">SSD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">wild type</strain>
        </source>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>cholesterol(in) = cholesterol(out)</text>
            <dbReference type="Rhea" id="RHEA:39747"/>
            <dbReference type="ChEBI" id="CHEBI:16113"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the patched family.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="ENSCINP00000008700.3"/>
        <property type="gene ID" value="ENSCING00000004214.3"/>
    </dbReference>
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    <dbReference type="HOGENOM" id="CLU_002359_0_0_1"/>
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    <dbReference type="OMA" id="WWFDVES"/>
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        <property type="component" value="Chromosome 2"/>
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        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0015485">
        <property type="term" value="F:cholesterol binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005319">
        <property type="term" value="F:lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="term" value="P:cholesterol homeostasis"/>
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        <property type="project" value="GO_Central"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:cholesterol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:intestinal cholesterol absorption"/>
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        <property type="project" value="GO_Central"/>
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        <property type="entry name" value="Multidrug efflux transporter AcrB transmembrane domain"/>
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        <property type="entry name" value="HMGCR/SNAP/NPC1-like_SSD"/>
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        <property type="entry name" value="NPC1-like"/>
    </dbReference>
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        <property type="entry name" value="NPC1_MLD"/>
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    <dbReference type="InterPro" id="IPR032190">
        <property type="entry name" value="NPC1_N"/>
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        <property type="entry name" value="SSD"/>
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    <dbReference type="NCBIfam" id="TIGR00917">
        <property type="entry name" value="2A060601"/>
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        <property type="entry name" value="NPC INTRACELLULAR CHOLESTEROL TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NPC INTRACELLULAR CHOLESTEROL TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22314">
        <property type="entry name" value="NPC1_MLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16414">
        <property type="entry name" value="NPC1_N"/>
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    <dbReference type="Pfam" id="PF12349">
        <property type="entry name" value="Sterol-sensing"/>
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    <keyword evidence="16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="10 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="14" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="12" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="5 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="248"/>
            <end position="274"/>
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            <begin position="1223"/>
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        <source>
            <dbReference type="SAM" id="Coils"/>
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    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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            <dbReference type="PROSITE" id="PS50156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000008700.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="1275" mass="141657" checksum="8E315B66F32BD7B1" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>F6YI57</accession>
    <name>F6YI57_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Hsp70-interacting protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">wild type</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">One HIP oligomer binds the ATPase domains of at least two HSC70 molecules dependent on activation of the HSC70 ATPase by HSP40. Stabilizes the ADP state of HSC70 that has a high affinity for substrate protein. Through its own chaperone activity, it may contribute to the interaction of HSC70 with various target proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homotetramer. Interacts with Hsc70 as well as DNAJ homologs and Hsp90.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM10 family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001429">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6YI57"/>
    <dbReference type="FunCoup" id="F6YI57">
        <property type="interactions" value="853"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000010745"/>
    <dbReference type="Ensembl" id="ENSCINT00000010745.3">
        <property type="protein sequence ID" value="ENSCINP00000010745.3"/>
        <property type="gene ID" value="ENSCING00000005227.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000170505"/>
    <dbReference type="HOGENOM" id="CLU_026202_2_0_1"/>
    <dbReference type="InParanoid" id="F6YI57"/>
    <dbReference type="OMA" id="NATIREC"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902494">
        <property type="term" value="C:catalytic complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030544">
        <property type="term" value="F:Hsp70 protein binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14438">
        <property type="entry name" value="Hip_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000112">
        <property type="entry name" value="FAM10 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="6.10.250.3420:FF:000001">
        <property type="entry name" value="Hsc70-interacting protein-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034649">
        <property type="entry name" value="Hip_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45883">
        <property type="entry name" value="HSC70-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45883:SF2">
        <property type="entry name" value="HSC70-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18253">
        <property type="entry name" value="HipN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 6" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="Hsp70-interacting protein N-terminal" evidence="8">
        <location>
            <begin position="4"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="6">
        <location>
            <begin position="154"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="40"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="260"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="74"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="265"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00037033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00064040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF18253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000010745.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="282" mass="31839" checksum="4A111D9112EF9236" modified="2012-04-18" version="2">ITKDKLSMLEQFIKLIKFNTSILHTPELKFLKDWLVDDMKATLPDPPATEANGDKPSYAEAASGKSAWSKKESSEEEEEEEDEESDLDIDMEGVIPGDTDPPQDMGDDSKEATEEEQDEAMKMRRLGMEAMSNGDNEGAIEHFTAGIKLDNTKTVLFVKRGTAYLRLAKPNAATRDAKKALEINPDSAAAYKVLGKADKFLGNWAEACHNFEVAQKIDYDDEIHDLLKEIKPKATKIREHTMARERKKKEKEIAARIKRVKKAQKAQEKAKKEQEERERVTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>F6YM31</accession>
    <name>F6YM31_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RNA/RNP complex-1-interacting phosphatase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Dual specificity protein phosphatase 11</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Phosphatase that interacts with RNA/RNP complex 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">wild type</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Possesses RNA 5'-triphosphatase and diphosphatase activities, but displays a poor protein-tyrosine phosphatase activity. In addition, has phosphatase activity with ATP, ADP and O-methylfluorescein phosphate (in vitro). Binds to RNA. May participate in nuclear mRNA metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Monomer. May interact with SFRS7 and SFRS9/SRP30C.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001132">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6YM31"/>
    <dbReference type="FunCoup" id="F6YM31">
        <property type="interactions" value="45"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000020998"/>
    <dbReference type="Ensembl" id="ENSCINT00000020998.3">
        <property type="protein sequence ID" value="ENSCINP00000020998.3"/>
        <property type="gene ID" value="ENSCING00000014482.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000168004"/>
    <dbReference type="HOGENOM" id="CLU_057587_0_0_1"/>
    <dbReference type="InParanoid" id="F6YM31"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004721">
        <property type="term" value="F:phosphoprotein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004651">
        <property type="term" value="F:polynucleotide 5'-phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17665">
        <property type="entry name" value="DSP_DUSP11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000064">
        <property type="entry name" value="RNA/RNP complex-1-interacting phosphatase homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051029">
        <property type="entry name" value="mRNA_Capping_Enz/RNA_Phosphat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10367:SF9">
        <property type="entry name" value="DUAL-SPECIFICITY PHOSPHATASE 11 (RNA_RNP COMPLEX 1-INTERACTING)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10367">
        <property type="entry name" value="MRNA-CAPPING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="13">
        <location>
            <begin position="34"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="14">
        <location>
            <begin position="103"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="176"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="300"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="210"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="220"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="304"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="339"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00054725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00065987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00068666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00076572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00080235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000020998.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="348" mass="41620" checksum="5D51A8CE3AC476A4" modified="2012-04-18" version="2">IMPRIPDRWLDYEKMGQVIEGTDILAMKVPLYEGITRNLPKKEHFNWKIAVDTAEERGHRIGTVVDLTYSTRYYQPRDFTNNGISHHKIFVPGQIIPPPKVVSDFTSIMSKFEKNKQSDNEVIAVHCTHGLNRTGYLICRYLIEEKKFKPKDAIEKFNSARGHKIERENYLKDLISLEKEKPVKTEQSPQKTKDNPPKPNNNSQKPKVSKTTDEVDLKLKHQNKLKHKLRKPDRDGYFRHNERQQNYYHHQPYPQQYKQQWYPDYHQERFNQYNHQGHNRGESWRKEPFNAFHKSFQQAPHYSHPHESGYNRGESRRKGPFNAFQESFQQAQHHYPPNRQWSNYSNWN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>F6YWP5</accession>
    <accession>A0A1W2WKR8</accession>
    <name>F6YWP5_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Delta(24)-sterol reductase</fullName>
            <ecNumber evidence="5">1.3.1.72</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="25">24-dehydrocholesterol reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="26">3-beta-hydroxysterol Delta-24-reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="29" type="primary">LOC100181233</name>
    </gene>
    <organism evidence="29 30">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="30" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="29" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="29">wild type</strain>
        </source>
    </reference>
    <reference evidence="29" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="29" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="24">Catalyzes the reduction of the delta-24 double bond of sterol intermediates during cholesterol biosynthesis. In addition to its cholesterol-synthesizing activity, can protect cells from oxidative stress by reducing caspase 3 activity during apoptosis induced by oxidative stress. Also protects against amyloid-beta peptide-induced apoptosis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>5alpha-cholest-8-en-3beta-ol + NADP(+) = zymosterol + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36399"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16608"/>
            <dbReference type="ChEBI" id="CHEBI:18252"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.3.1.72"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="23">
            <dbReference type="Rhea" id="RHEA:36401"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>lanosterol + NADPH + H(+) = 24,25-dihydrolanosterol + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:33919"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16521"/>
            <dbReference type="ChEBI" id="CHEBI:28113"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:33920"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the FAD-binding oxidoreductase/transferase type 4 family.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.72" evidence="5"/>
    <dbReference type="EMBL" id="EAAA01002575">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002130771.1">
        <property type="nucleotide sequence ID" value="XM_002130735.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6YWP5"/>
    <dbReference type="FunCoup" id="F6YWP5">
        <property type="interactions" value="8"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000024565"/>
    <dbReference type="Ensembl" id="ENSCINT00000024811.2">
        <property type="protein sequence ID" value="ENSCINP00000024565.2"/>
        <property type="gene ID" value="ENSCING00000008369.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100181233"/>
    <dbReference type="KEGG" id="cin:100181233"/>
    <dbReference type="GeneTree" id="ENSGT00390000008338"/>
    <dbReference type="HOGENOM" id="CLU_025883_4_0_1"/>
    <dbReference type="InParanoid" id="F6YWP5"/>
    <dbReference type="OMA" id="WVGRSAF"/>
    <dbReference type="OrthoDB" id="415825at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000246">
        <property type="term" value="F:Delta24(24-1) sterol reductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050614">
        <property type="term" value="F:Delta24-sterol reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008203">
        <property type="term" value="P:cholesterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008202">
        <property type="term" value="P:steroid metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.465.10:FF:000032">
        <property type="entry name" value="Delta(24)-sterol reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040165">
        <property type="entry name" value="Diminuto-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10801">
        <property type="entry name" value="24-DEHYDROCHOLESTEROL REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10801:SF0">
        <property type="entry name" value="DELTA(24)-STEROL REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0153">Cholesterol metabolism</keyword>
    <keyword evidence="12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="13" id="KW-0274">FAD</keyword>
    <keyword evidence="9" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="17" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="18" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="19 27" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0521">NADP</keyword>
    <keyword evidence="16" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="21" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="20" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="10 27" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 27" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="27">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="28">
        <location>
            <begin position="48"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00019086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00051033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00052927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00056986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00078485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00080612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000024565.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="513" mass="59609" checksum="5A7AAFDFEC80F22D" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>F6YX67</accession>
    <name>F6YX67_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">NEDD8-activating enzyme E1 catalytic subunit</fullName>
            <ecNumber evidence="8 11">6.2.1.64</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100183602</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">wild type</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Catalytic subunit of the dimeric E1 enzyme, which activates NEDD8.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 11">
            <text>ATP + [NEDD8 protein] + [E1 NEDD8-activating enzyme]-L-cysteine = AMP + diphosphate + [E1 NEDD8-activating enzyme]-S-[NEDD8 protein]-yl-L-cysteine.</text>
            <dbReference type="EC" id="6.2.1.64"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 11">Protein modification; protein neddylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the ubiquitin-activating E1 family. UBA3 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.64" evidence="8 11"/>
    <dbReference type="EMBL" id="EAAA01000397">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6YX67"/>
    <dbReference type="FunCoup" id="F6YX67">
        <property type="interactions" value="1249"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000012570"/>
    <dbReference type="Ensembl" id="ENSCINT00000012570.3">
        <property type="protein sequence ID" value="ENSCINP00000012570.3"/>
        <property type="gene ID" value="ENSCING00000006095.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00550000074831"/>
    <dbReference type="HOGENOM" id="CLU_013325_13_1_1"/>
    <dbReference type="InParanoid" id="F6YX67"/>
    <dbReference type="OMA" id="PYLENYM"/>
    <dbReference type="UniPathway" id="UPA00885"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019781">
        <property type="term" value="F:NEDD8 activating enzyme activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045116">
        <property type="term" value="P:protein neddylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01488">
        <property type="entry name" value="Uba3_RUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.520:FF:000001">
        <property type="entry name" value="NEDD8-activating enzyme E1 catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.290.20:FF:000001">
        <property type="entry name" value="NEDD8-activating enzyme E1 catalytic subunit, variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.520">
        <property type="entry name" value="Ubiquitin activating enzymes (Uba3). Chain: B, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.20">
        <property type="entry name" value="Ubiquitin-like 2 activating enzyme e1b. Chain: B, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014929">
        <property type="entry name" value="E2-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023318">
        <property type="entry name" value="Ub_act_enz_dom_a_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030468">
        <property type="entry name" value="Uba3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033127">
        <property type="entry name" value="UBQ-activ_enz_E1_Cys_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953:SF6">
        <property type="entry name" value="NEDD8-ACTIVATING ENZYME E1 CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08825">
        <property type="entry name" value="E2_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01181">
        <property type="entry name" value="E2_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00865">
        <property type="entry name" value="UBIQUITIN_ACTIVAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="E2 binding" evidence="12">
        <location>
            <begin position="352"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="10">
        <location>
            <position position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU368009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000012570.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="446" mass="49769" checksum="5846CE80E8C05969" modified="2012-04-18" version="2">MEVDDCDDGARWSGRWNHLQKILERNGPFVHPDFEPSPDILDFMLNSCKVLVVGAGGLGCELLKDLSLMGLRHIHVIDMDTIDLSNLNRQFLFRQCDIGKPKAEVAASFINKRVKGSKTFSHFCRIQDHDSSFYKQFHIVVCGLDSIVARRWINGMLISLLEYDEDGNLDPSSLIPLVDGGTEGFKGNARVILPGMNACMDCTLDLYPPQVNFPMCTIAHTPRQPEHCIEYVKIFQWEKEKPFGDIAIDGDDPDHIKWICKAASTRADEYGIQGVNYRLTQGVIKRIIPAVASTNAVIAAACANEVFKLATSCSIPLNNYMVFNDTNGVYTYTFEAERNEQCLACNNTPRNLSFKPDALLREVVDFLCQDNSLQMKSPGLTTTIEGLNKTLYMKNIPSLEAATRPNLSKSLKDLGIVDGQEVIVADVTTPNSLRFKIKFNDDVTSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F6YXS6</accession>
    <name>F6YXS6_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="F6YXS6"/>
    <dbReference type="Ensembl" id="ENSCINT00000000589.3">
        <property type="protein sequence ID" value="ENSCINP00000000589.3"/>
        <property type="gene ID" value="ENSCING00000000325.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000009287"/>
    <dbReference type="HOGENOM" id="CLU_1437477_0_0_1"/>
    <dbReference type="InParanoid" id="F6YXS6"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="75"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="158"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="33"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="159"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="180"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000000589.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="189" mass="20547" checksum="5235A29FDB1ED117" modified="2012-04-18" version="2">MGASPPPRMPLLKHKPPVHADQPTNTLKTRHVSGSAASSTTTTSSISQDNIQPASIEDMVQHLNGLVGLTREEIELLNENPGNPPSYPSSTTGSVMSSYPPSVEPPRSRKPSALQGGIARPSSLPGRRIDRIHLSGRSRTPMMMPPSYEMKYKIPLDLQPSQFLQPPDTSSDEEDFDSFKINSGCETET</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>F6ZA28</accession>
    <name>F6ZA28_CIOIN</name>
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        <recommendedName>
            <fullName evidence="1">Calcineurin-like phosphoesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="F6ZA28"/>
    <dbReference type="Ensembl" id="ENSCINT00000026960.2">
        <property type="protein sequence ID" value="ENSCINP00000026714.2"/>
        <property type="gene ID" value="ENSCING00000014879.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000010260"/>
    <dbReference type="HOGENOM" id="CLU_025443_3_3_1"/>
    <dbReference type="InParanoid" id="F6ZA28"/>
    <dbReference type="OMA" id="HENGRTQ"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07385">
        <property type="entry name" value="MPP_YkuE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051158">
        <property type="entry name" value="Metallophosphoesterase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31302:SF0">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN WITH METALLOPHOSPHOESTERASE DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31302">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN WITH METALLOPHOSPHOESTERASE DOMAIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="1">
        <location>
            <begin position="2"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000026714.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="198" mass="22167" checksum="9A9BCF6EA3ACAC85" modified="2012-04-18" version="2">MAGDLTDATVDAMGEAAEPLSRIIAPYGKYFSTGNHEYYTGDVENWFKLLESFDFHILHNSNVKIHDKSDDKQWICMAGVDDIQADQIGYTGHGMNLKQAYEGCDEKHSTILVAHQPKAAKFALDSDYKIQLVLSGHTHGGQMYPIIWLAYFLNPYLSGLYQHGASSYVYVSQGSVYYGFPLRLGSYPEIPNIVLRSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>F6ZGS5</accession>
    <accession>A0A1W2W7D7</accession>
    <name>F6ZGS5_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">NADH-cytochrome b5 reductase</fullName>
            <ecNumber evidence="13">1.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC100176772</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>2 Fe(III)-[cytochrome b5] + NADH = 2 Fe(II)-[cytochrome b5] + NAD(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46680"/>
            <dbReference type="Rhea" id="RHEA-COMP:10438"/>
            <dbReference type="Rhea" id="RHEA-COMP:10439"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.6.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12 13">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.6.2.2" evidence="13"/>
    <dbReference type="RefSeq" id="XP_002126207.1">
        <property type="nucleotide sequence ID" value="XM_002126171.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6ZGS5"/>
    <dbReference type="FunCoup" id="F6ZGS5">
        <property type="interactions" value="357"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000012527"/>
    <dbReference type="Ensembl" id="ENSCINT00000012527.3">
        <property type="protein sequence ID" value="ENSCINP00000012527.3"/>
        <property type="gene ID" value="ENSCING00000016482.2"/>
    </dbReference>
    <dbReference type="GeneID" id="100176772"/>
    <dbReference type="KEGG" id="cin:100176772"/>
    <dbReference type="GeneTree" id="ENSGT00940000153962"/>
    <dbReference type="HOGENOM" id="CLU_003827_9_2_1"/>
    <dbReference type="InParanoid" id="F6ZGS5"/>
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        <property type="term" value="P:sterol biosynthetic process"/>
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        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
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        <property type="entry name" value="Translation factors"/>
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        <property type="entry name" value="CBR-like"/>
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        <property type="entry name" value="Cbr1-like_FAD-bd_dom"/>
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        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
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        <property type="entry name" value="NADH-CYTOCHROME B5 REDUCTASE"/>
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        <property type="entry name" value="NADH-CYTOCHROME B5 REDUCTASE"/>
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        <property type="entry name" value="FAD_binding_6"/>
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        <property type="entry name" value="NAD_binding_1"/>
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    <dbReference type="PRINTS" id="PR00406">
        <property type="entry name" value="CYTB5RDTASE"/>
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        <property type="entry name" value="FPNCR"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12 13" id="KW-0274">FAD</keyword>
    <keyword evidence="4 12 13" id="KW-0285">Flavoprotein</keyword>
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    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
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    <keyword evidence="9 13" id="KW-0520">NAD</keyword>
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    <keyword evidence="11" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="8" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="10" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="14" id="KW-0812">Transmembrane</keyword>
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        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
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    <feature type="domain" description="FAD-binding FR-type" evidence="15">
        <location>
            <begin position="47"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
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        <location>
            <position position="100"/>
        </location>
        <ligand>
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        <location>
            <position position="101"/>
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        <location>
            <position position="116"/>
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        <ligand>
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        <location>
            <position position="118"/>
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        <ligand>
            <name>FAD</name>
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        <location>
            <position position="121"/>
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        <ligand>
            <name>FAD</name>
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        <location>
            <position position="133"/>
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        <ligand>
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            <position position="134"/>
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            <position position="135"/>
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        <ligand>
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            <position position="192"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00006105"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022630"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023011"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
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        <source>
            <dbReference type="PIRSR" id="PIRSR601834-1"/>
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    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU361226"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000012527.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="308" mass="34403" checksum="340DC8EEAB12AEF2" modified="2012-04-18" version="2">MVADATNQTSSSVFTFGITAAVIAVTLAYLKKKRDENAVKKTLLDPEVKYALKLIAKERITHDTRKFVFALPSENHILGLTVGKHIFLSARVDGKLVVRPYTPVTSDDDKGHMDLVIKVYFKDTHPKFPEGGKMSQYLEALPIGGTIDVRGPNGLIEYRTKSVFSVRYEKNGFSYAKRAKEVAMIAGGTGITPMLQIIRQCLKDPSDHTKLSLLYANQTEDDILVRKELEECAKADPNHFKLWYTLDRPPADWKYSTGFIDEDMIRDNLPAPGIDTLVLLCGPPPMIKFACLPNLEKVGHQSGRICTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>F6ZJE6</accession>
    <name>F6ZJE6_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AIMP2 thioredoxin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="F6ZJE6"/>
    <dbReference type="FunCoup" id="F6ZJE6">
        <property type="interactions" value="185"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000027815"/>
    <dbReference type="Ensembl" id="ENSCINT00000028061.2">
        <property type="protein sequence ID" value="ENSCINP00000027815.2"/>
        <property type="gene ID" value="ENSCING00000015854.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015826"/>
    <dbReference type="HOGENOM" id="CLU_966295_0_0_1"/>
    <dbReference type="InParanoid" id="F6ZJE6"/>
    <dbReference type="OMA" id="LCQHYRV"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017101">
        <property type="term" value="C:aminoacyl-tRNA synthetase multienzyme complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042360">
        <property type="entry name" value="AIMP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041503">
        <property type="entry name" value="AIMP2_thioredoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13438">
        <property type="entry name" value="AMINOACYL TRNA SYNTHASE COMPLEX-INTERACTING MULTIFUNCTIONAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13438:SF2">
        <property type="entry name" value="AMINOACYL TRNA SYNTHASE COMPLEX-INTERACTING MULTIFUNCTIONAL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18569">
        <property type="entry name" value="Thioredoxin_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AIMP2 thioredoxin-like" evidence="4">
        <location>
            <begin position="82"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF18569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000027815.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="237" mass="26768" checksum="E93762C7D7F41C85" modified="2012-04-18" version="2">MYSAKPHFEYNSTNVQLPDVMYKASSMVTICDNTGDNNVTQELTSLKQRQSDLLRGLDDMIERVNKMKSMVTTTTNKGNNAEFRDIVIHACPSQPPLSLKYLIKMLRQRFKVLVKVHGHSSVTNHMTSFLTNEETTNRNHYDIIITLIWKSAPDDPITHLHPSSSPIVGESNLARLVGRMLGYYDNTPTSFLIITTTDVIYDACDVINNIGSTKKQRQDALQTLGGVLNNNQWFMGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>F6ZNG2</accession>
    <name>F6ZNG2_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">wild type</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01002660">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="F6ZNG2"/>
    <dbReference type="STRING" id="7719.ENSCINP00000006941"/>
    <dbReference type="Ensembl" id="ENSCINT00000006941.3">
        <property type="protein sequence ID" value="ENSCINP00000006941.3"/>
        <property type="gene ID" value="ENSCING00000003384.3"/>
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    <dbReference type="GeneTree" id="ENSGT00940000162287"/>
    <dbReference type="HOGENOM" id="CLU_951657_0_0_1"/>
    <dbReference type="InParanoid" id="F6ZNG2"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 8"/>
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    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:003297">
        <property type="entry name" value="Si:ch211-266d19.4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Classic Zinc Finger"/>
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    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
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    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24393:SF34">
        <property type="entry name" value="PR_SET DOMAIN 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24393">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="5"/>
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    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="5"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="20"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="136"/>
            <end position="164"/>
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    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="165"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="203"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="260"/>
            <end position="288"/>
        </location>
    </feature>
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        <location>
            <begin position="36"/>
            <end position="55"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="46"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="8">
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            <dbReference type="PROSITE" id="PS50157"/>
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            <dbReference type="Ensembl" id="ENSCINP00000006941.3"/>
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    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="293" mass="32965" checksum="0A76219867716082" modified="2012-04-18" version="2">MFENAAMLQHHVMSTHPQPFWCTVCGEGFSQASQLQSHLQVRHSDNNNNQQPTNPITAVIQSTNQSQGVSSNRPMGLVGQQIDLNQLPDNLNKSCLECGAVIDKTIKRHVCHGVKFQQQTALPGVAAVALATERPHLCTKCGKGFKTATHLKQHVRCVHTNDRPHKCTQCSKTFARMSDLNRHRRGVHERDKGTSKSLRMLSLKCTQCGATFPETIQLKRHVLTVHMNQLPCYKCADCQEGFADESQFKSHICYEEERPHVCHICSKRFSAPTQLRRHVRSAHNPGNPQTTHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>F7A2D4</accession>
    <name>F7A2D4_CIOIN</name>
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        <recommendedName>
            <fullName evidence="7">BPTI/Kunitz inhibitor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">wild type</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the venom Kunitz-type family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002467">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F7A2D4"/>
    <dbReference type="STRING" id="7719.ENSCINP00000015829"/>
    <dbReference type="Ensembl" id="ENSCINT00000015829.3">
        <property type="protein sequence ID" value="ENSCINP00000015829.3"/>
        <property type="gene ID" value="ENSCING00000007714.3"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1028678_0_0_1"/>
    <dbReference type="InParanoid" id="F7A2D4"/>
    <dbReference type="OMA" id="MNRCELF"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00109">
        <property type="entry name" value="Kunitz-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.410.10">
        <property type="entry name" value="Pancreatic trypsin inhibitor Kunitz domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002223">
        <property type="entry name" value="Kunitz_BPTI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036880">
        <property type="entry name" value="Kunitz_BPTI_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020901">
        <property type="entry name" value="Prtase_inh_Kunz-CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050098">
        <property type="entry name" value="TFPI/VKTCI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000716">
        <property type="entry name" value="Thyroglobulin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036857">
        <property type="entry name" value="Thyroglobulin_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10083:SF217">
        <property type="entry name" value="BOOPHILIN-H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10083">
        <property type="entry name" value="KUNITZ-TYPE PROTEASE INHIBITOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00014">
        <property type="entry name" value="Kunitz_BPTI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00086">
        <property type="entry name" value="Thyroglobulin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00759">
        <property type="entry name" value="BASICPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00131">
        <property type="entry name" value="KU"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57362">
        <property type="entry name" value="BPTI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57610">
        <property type="entry name" value="Thyroglobulin type-1 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00280">
        <property type="entry name" value="BPTI_KUNITZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50279">
        <property type="entry name" value="BPTI_KUNITZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0800">Toxin</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
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            <end position="271"/>
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        <location>
            <begin position="168"/>
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        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50279"/>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000015829.3"/>
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    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    </evidence>
    <sequence length="271" mass="30487" checksum="1EA6570B549FDCD4" modified="2012-04-18" version="2">MKMTSTLLKLAVTVAVVVVSGCMASPVPADDVGCFAARNAVFLDIRRLLRGEWPYCDEGGLWKNVQCGINSCYCAYVDTGAQIQNLNLRTLYYSYDQLMGMCPIRFQANRDDACRVPHLNYPCNTSAPKYIYNQESGECEETPSSRCYGFMSLSECQSQCAPVVHDRCSHPYDIGHSCSDHTPTGRYFHNPSMNRCELFYYVGCGGNTNNYRTLIECERTCLPQPIVTITVQVEGRNTHSLEVPSWVLDDYSLVSDNVNQISQVIMDRVRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">wild type</strain>
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    <reference evidence="5" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <dbReference type="InParanoid" id="F7AEP7"/>
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        <property type="component" value="Chromosome 11"/>
    </dbReference>
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        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="MATRIN 3/NUCLEAR PROTEIN 220-RELATED"/>
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    </dbReference>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Di Gregorio A."/>
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                <person name="Goodstein D.M."/>
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                <person name="Hastings K.E."/>
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                <person name="Huang W."/>
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                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
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                <person name="Haga S."/>
                <person name="Hayashi H."/>
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                <person name="Imai K.S."/>
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                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
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                <person name="Nomoto H."/>
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                <person name="Oishi K."/>
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                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
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                <person name="Levine M."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
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    <feature type="chain" id="PRO_5003353394" evidence="1">
        <location>
            <begin position="24"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000029035.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="118" mass="13571" checksum="16C4508E726EE836" modified="2011-07-27" version="1">MRNTVFTVVLVVVCFHTLYGSDAQLSELNQDASNRHRRSQPTYHLIDTCMRRELDNTIRGGVEVPWTYRATYFLVQTLYANCNVMSLQPEGLIACTNRTVNFEVERFGRIVADCMAVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F7AUD7</accession>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
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                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
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                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
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                <person name="Richardson P."/>
                <person name="Lucas S."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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            <strain evidence="2">wild type</strain>
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    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
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                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
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        <source>
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<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
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            <taxon>Eukaryota</taxon>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">wild type</strain>
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    <reference evidence="11" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="11" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
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    <comment type="similarity">
        <text evidence="2 8">Belongs to the TGF-beta family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="Ensembl" id="ENSCINT00000008362.3">
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        <property type="gene ID" value="ENSCING00000004054.3"/>
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    <dbReference type="HOGENOM" id="CLU_020515_4_2_1"/>
    <dbReference type="InParanoid" id="F7B7A4"/>
    <dbReference type="OMA" id="NDYHHSK"/>
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        <property type="component" value="Chromosome 2"/>
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        <property type="project" value="GO_Central"/>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0007178">
        <property type="term" value="P:cell surface receptor protein serine/threonine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="entry name" value="TGF-b_propeptide"/>
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        <property type="entry name" value="BMP-LIKE PROTEIN UNC-129"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="INHIBINA"/>
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    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
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    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
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    </evidence>
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        <source>
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        <source>
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    </evidence>
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        <source>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51362"/>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F7BFZ7</accession>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
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            <strain evidence="2">wild type</strain>
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F7BFZ7"/>
    <dbReference type="Ensembl" id="ENSCINT00000026935.2">
        <property type="protein sequence ID" value="ENSCINP00000026689.2"/>
        <property type="gene ID" value="ENSCING00000014860.2"/>
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    <dbReference type="GeneTree" id="ENSGT00390000004507"/>
    <dbReference type="HOGENOM" id="CLU_1325978_0_0_1"/>
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    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 6"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="103"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="27"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
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            <begin position="53"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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            <begin position="103"/>
            <end position="132"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000026689.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
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            <fullName evidence="3">Coiled-coil domain-containing protein 24</fullName>
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        <name type="scientific">Ciona intestinalis</name>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">wild type</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="F7BM33"/>
    <dbReference type="STRING" id="7719.ENSCINP00000024447"/>
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        <property type="entry name" value="CCDC24"/>
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        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 24"/>
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        <property type="entry name" value="CCDC24"/>
        <property type="match status" value="1"/>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000024447.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="227" mass="26200" checksum="68C4C11694FC5163" modified="2012-04-18" version="2">MEVNGERLEPFKPPFSVLELIRNTVAPEELPVIHSYLGESTIEYCKDLRNEMETLLEIWRDIGDRTEMPTVSKPLSNPLPDPPIMREMLKKEIQILVKGVQDRHTVSGDQTLNENIFENNFILHFSYFSERPSTPIRHYTSETSYTTSPVKLKHVRSNINVKDIDKVALILKEAIQKECAALEHDIEFLQKCVEEEHDLVASPPATPMQEPSLNKLREVRKLLENQV</sequence>
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    <accession>H2XJF8</accession>
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        <recommendedName>
            <fullName evidence="9">BZIP domain-containing protein</fullName>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
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    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
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                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
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                <person name="Harafuji N."/>
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                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
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                <person name="Inaba K."/>
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                <person name="Lee B.I."/>
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                <person name="Manohar C."/>
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                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
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                <person name="Richardson P."/>
                <person name="Lucas S."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
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                <person name="Ueno K."/>
                <person name="Yamada L."/>
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                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
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                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">wild type</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="10" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
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        <text evidence="2">Belongs to the bZIP family. ATF subfamily.</text>
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    <dbReference type="PANTHER" id="PTHR45996">
        <property type="entry name" value="AGAP001464-PB"/>
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    <dbReference type="PANTHER" id="PTHR45996:SF3">
        <property type="entry name" value="CREB-H TRANSCRIPTION FACTOR HOMOLOG LET-607"/>
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    <dbReference type="Pfam" id="PF00170">
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    <dbReference type="SMART" id="SM00338">
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS50217">
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        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
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                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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    </reference>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="10">wild type</strain>
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>S-methyl-5'-thioadenosine + phosphate = 5-(methylsulfanyl)-alpha-D-ribose 1-phosphate + adenine</text>
            <dbReference type="Rhea" id="RHEA:11852"/>
            <dbReference type="ChEBI" id="CHEBI:16708"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58533"/>
            <dbReference type="EC" id="2.4.2.28"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:11853"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>adenosine + H2O + H(+) = inosine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:24408"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16335"/>
            <dbReference type="ChEBI" id="CHEBI:17596"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
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            <dbReference type="Rhea" id="RHEA:24409"/>
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    <comment type="catalytic activity">
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            <text>adenosine + phosphate = alpha-D-ribose 1-phosphate + adenine</text>
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            <dbReference type="ChEBI" id="CHEBI:16335"/>
            <dbReference type="ChEBI" id="CHEBI:16708"/>
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            <dbReference type="ChEBI" id="CHEBI:57720"/>
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            <dbReference type="Rhea" id="RHEA:27643"/>
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            <text>inosine + phosphate = alpha-D-ribose 1-phosphate + hypoxanthine</text>
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            <dbReference type="ChEBI" id="CHEBI:17368"/>
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    <comment type="similarity">
        <text evidence="2">Belongs to the purine nucleoside phosphorylase YfiH/LACC1 family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
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                <person name="Lee B.I."/>
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                <person name="Manohar C."/>
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                <person name="Mochizuki Y."/>
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                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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    <reference evidence="11" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="11">wild type</strain>
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    <reference evidence="11" key="3">
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        <scope>IDENTIFICATION</scope>
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    <reference evidence="11" key="4">
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            <authorList>
                <consortium name="Ensembl"/>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="molecule type" value="Genomic_DNA"/>
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            <begin position="1"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000030336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="253" mass="28605" checksum="2668D794DA42D955" modified="2012-04-18" version="1">MFSTLHEKLSPKNRMNEGTSKKWLSNTERAVGEFRTLSSRLADHVATVELDRTASPTSHASMDDVSTSYKIENTPTIFEDKSRKDVIRRKEQRTNKSMSPEVRRRGGVCINAPYSHTTGRIPDNKRFSQNSSSESEMDLFGSVDSLDKTIMELQSGFHFSGPNILSTTTGNHPPGLSPRFLRKYNVTKTTLPHNNYHKVQTRPTSLPQTENQDSVFNSLDKKPYSCWLDDSSNERNATGKMIASWQDMPTTVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>H2XLK3</accession>
    <name>H2XLK3_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
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    </protein>
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        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
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                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
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                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
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                <person name="Harafuji N."/>
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                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
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                <person name="Makabe K.W."/>
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                <person name="Mochizuki Y."/>
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                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
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                <person name="Oishi K."/>
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                <person name="Sasaki A."/>
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                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
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    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
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                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
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        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the helicase family. RLR subfamily.</text>
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    <dbReference type="STRING" id="7719.ENSCINP00000031621"/>
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        <property type="gene ID" value="ENSCING00000009744.3"/>
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    <dbReference type="GeneTree" id="ENSGT00940000153173"/>
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        <property type="term" value="P:antiviral innate immune response"/>
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        <property type="term" value="P:cytoplasmic pattern recognition receptor signaling pathway"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
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        <property type="entry name" value="RIG-I-like receptor, C-terminal regulatory domain"/>
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        <property type="entry name" value="RIG-I-like_C"/>
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        <property type="entry name" value="RLR_C_sf"/>
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        <property type="entry name" value="RLR_Helicase"/>
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    <dbReference type="PANTHER" id="PTHR14074:SF16">
        <property type="entry name" value="ANTIVIRAL INNATE IMMUNE RESPONSE RECEPTOR RIG-I"/>
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    <dbReference type="PANTHER" id="PTHR14074">
        <property type="entry name" value="HELICASE WITH DEATH DOMAIN-RELATED"/>
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    <dbReference type="Pfam" id="PF00271">
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        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF18119">
        <property type="entry name" value="RIG-I_C"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF11648">
        <property type="entry name" value="RIG-I_C-RD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
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    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51789">
        <property type="entry name" value="RLR_CTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
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    <keyword evidence="12" id="KW-0391">Immunity</keyword>
    <keyword evidence="5" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="16">
        <location>
            <begin position="14"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="17">
        <location>
            <begin position="360"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="domain" description="RLR CTR" evidence="18">
        <location>
            <begin position="546"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006866"/>
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    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00049390"/>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="PROSITE" id="PS51789"/>
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    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000031621.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>H2XQ36</accession>
    <name>H2XQ36_CIOIN</name>
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        <recommendedName>
            <fullName evidence="7">Zinc transporter ZIP13</fullName>
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        <alternativeName>
            <fullName evidence="8">Solute carrier family 39 member 13</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Zrt- and Irt-like protein 13</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">wild type</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>Zn(2+)(in) = Zn(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29351"/>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="EAAA01002595">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XQ36"/>
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        <property type="interactions" value="69"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000031770"/>
    <dbReference type="Ensembl" id="ENSCINT00000031616.1">
        <property type="protein sequence ID" value="ENSCINP00000031770.1"/>
        <property type="gene ID" value="ENSCING00000023228.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157349"/>
    <dbReference type="HOGENOM" id="CLU_015114_0_2_1"/>
    <dbReference type="InParanoid" id="H2XQ36"/>
    <dbReference type="OMA" id="SCIAIHA"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006882">
        <property type="term" value="P:intracellular zinc ion homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071577">
        <property type="term" value="P:zinc ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003689">
        <property type="entry name" value="ZIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16950">
        <property type="entry name" value="ZINC TRANSPORTER SLC39A7 HISTIDINE-RICH MEMBRANE PROTEIN KE4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16950:SF16">
        <property type="entry name" value="ZINC TRANSPORTER ZIP13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02535">
        <property type="entry name" value="Zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0864">Zinc transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="16"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="57"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="94"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="261"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="295"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="139"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="149"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00042542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00042972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000031770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="312" mass="33611" checksum="24B33A65F62EC85E" modified="2012-04-18" version="1">MKSANSTLLDYSRFEATVLALLSAGLVGMSGIFPLIILPKINGGLNKKDGSVQLQRLLSFAVGGLLGDVFLHLLPESWDQLNNNSESGGSHTHWPAVGNGLWVLIGLISFCLLEKLFPDDKPEMSDDLTVSEHTDVFATSKQNGSKNTKHYETNKERSSSTQIKTSGYLNLLANCVDNFTHGLAVGGSYLVSRRVGVLTTLAILLHEIPHEVGDFAILLRAGFDRWQAAKLQILTAAGGLLGAAVALLAESAKSAGDRVSWILPFTSGGFIYIALCTCVPDLLKEKSPRESCIQILYIILAIVTMHLVSYVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>H2XQ52</accession>
    <name>H2XQ52_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the nephronectin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="H2XQ52"/>
    <dbReference type="STRING" id="7719.ENSCINP00000031786"/>
    <dbReference type="Ensembl" id="ENSCINT00000032969.1">
        <property type="protein sequence ID" value="ENSCINP00000031786.1"/>
        <property type="gene ID" value="ENSCING00000018863.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163310"/>
    <dbReference type="HOGENOM" id="CLU_644873_0_0_1"/>
    <dbReference type="InParanoid" id="H2XQ52"/>
    <dbReference type="OMA" id="TENGNCQ"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000031">
        <property type="entry name" value="neurogenic locus notch homolog protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013032">
        <property type="entry name" value="EGF-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000998">
        <property type="entry name" value="MAM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051277">
        <property type="entry name" value="SEZ6_CSMD_C4BPB_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45656">
        <property type="entry name" value="PROTEIN CBR-CLEC-78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45656:SF4">
        <property type="entry name" value="PROTEIN CBR-CLEC-78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12661">
        <property type="entry name" value="hEGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00629">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00137">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50060">
        <property type="entry name" value="MAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2 6" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0768">Sushi</keyword>
    <feature type="domain" description="Sushi" evidence="10">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="10">
        <location>
            <begin position="65"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="10">
        <location>
            <begin position="124"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="10">
        <location>
            <begin position="183"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="MAM" evidence="9">
        <location>
            <begin position="243"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="8">
        <location>
            <begin position="389"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="35"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="94"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="415"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000031786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="426" mass="47721" checksum="1BA202CF029935AB" modified="2012-04-18" version="1">MTCFNPDLICDPLEAFENGDILGRRGHGETVTYRCNFGYHLDGDSRRTCWADKKWTGRAPRCRRGCRPPRPTDTGYANSFDNFYEVGAVVNYVCRIGYIISGDAQSRCQENGWWSELQFRCRIIVCDEPPLVANSRRNGTSFEFGRRVRYICNQGYELASESGWLLCDTTGEWFGTVPRCDPVSCLAPQNPVNGRVITNGVQFTYGNRVRYSCSRQFKLVGPSESTCNADRNWSPAPTCEVDIDCNFQDTSRATCGWLNNGVAWDQVIHGADVYYHARFSRGNVGTTSKSILISPFVSASSNQCFKFHYRLVQISSSSDFVVNIIDGNSRSQIWSCNESDELNAWLNTDIAILPRQTNYQIEIVVAKSTVSSIRIDIDNLKVTENGNCQNECEPVSPCLNGGQCLNRIGSFKCICPAGYRGLTCSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>H2XRV3</accession>
    <name>H2XRV3_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Fibronectin type-III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
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                <person name="Lindquist E."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="5">wild type</strain>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <location evidence="1">Membrane</location>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <source>
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    </evidence>
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            <dbReference type="Proteomes" id="UP000008144"/>
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    <accession>H2XTB6</accession>
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            <fullName evidence="9">Transmembrane anterior posterior transformation protein 1 homolog</fullName>
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        <name type="scientific">Ciona intestinalis</name>
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        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
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            <taxon>Phlebobranchia</taxon>
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    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">wild type</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAPT1 family.</text>
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    <dbReference type="EMBL" id="EAAA01002629">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="STRING" id="7719.ENSCINP00000032900"/>
    <dbReference type="Ensembl" id="ENSCINT00000033542.1">
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        <property type="gene ID" value="ENSCING00000023254.1"/>
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        <property type="project" value="GO_Central"/>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008010">
        <property type="entry name" value="Tatp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13317">
        <property type="entry name" value="TRANSMEMBRANE ANTERIOR POSTERIOR TRANSFORMATION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13317:SF4">
        <property type="entry name" value="TRANSMEMBRANE ANTERIOR POSTERIOR TRANSFORMATION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DUF747"/>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
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                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
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                <person name="Rigoutsos I."/>
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                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
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                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <dbReference type="STRING" id="7719.ENSCINP00000033107"/>
    <dbReference type="Ensembl" id="ENSCINT00000035961.1">
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        <property type="component" value="Unassembled WGS sequence"/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
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                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="15" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="15">wild type</strain>
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    <reference evidence="15" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="15" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <cofactor evidence="1">
            <name>Mg(2+)</name>
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        <text evidence="3">Belongs to the XPG/RAD2 endonuclease family. XPG subfamily.</text>
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</entry>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
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                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
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                <person name="Yoshizaki F."/>
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                <person name="Hyatt P.D."/>
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                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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    </reference>
    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
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                <person name="Suzuki M.M."/>
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                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
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                <person name="Richardson P."/>
                <person name="Lucas S."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="protein sequence ID" value="ENSCINP00000034201.1"/>
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    <dbReference type="OMA" id="NDMIAYW"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002018">
        <property type="entry name" value="CarbesteraseB"/>
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        <property type="entry name" value="CARBOXYLIC ESTER HYDROLASE"/>
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    <dbReference type="PANTHER" id="PTHR45570:SF1">
        <property type="entry name" value="CARBOXYLIC ESTER HYDROLASE"/>
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        <property type="match status" value="1"/>
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        <source>
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    </evidence>
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        <source>
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        <source>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
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                <person name="Tassy O."/>
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                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="3" key="2">
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                <consortium name="Ensembl"/>
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            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <dbReference type="STRING" id="7719.ENSCINP00000034389"/>
    <dbReference type="Ensembl" id="ENSCINT00000031454.1">
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        <property type="entry name" value="Thioredoxin-like_sf"/>
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            <dbReference type="Pfam" id="PF00085"/>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    <sequence length="429" mass="48469" checksum="8FD2C15EE7E8A7AE" modified="2012-04-18" version="1">MSVHSSPQTRSAPQPTPIITHPPIHDLPTGSLVHLQYLLSISEISLVLYYAPWCERSFSSVKELNKTAQVFHKQVSFVAINCWWDQGECRGNNNFQSFPHLYLHHRSLKTPVHYNGPHTHQYFARFVSKVLSPFTYLGTLDVVEGYLNNGKTSIIASAGVNLTSNELLQQFLQISLQSLSKFPSWAIQMGLITSPFLHTTLGMKEGYMRIYNGGRGVGEISLNSTWEVVEGWMEKKKDPIVVPRILSSGQKSNLFFQVLNKGPTLVAFLPSLDDVTVFEEINTIYNKNCHQVSTHCSSIVTSQPSICNVCLQRDSHPRTPHNEEIFFRRLLKLGYFEALATQSHSCVQSRSFYDKHRTRLNICCGNIHKNHTKINGLGCRSNCTLQFLMVDSTQYPALATAVKVDDTKSNAVIIDIHNEMEYLMKGSFT</sequence>
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        <name evidence="2" type="primary">LOC100181390</name>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
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                <person name="Miura S."/>
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                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
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    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <evidence type="ECO:0000256" key="1">
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        <source>
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        </source>
    </evidence>
    <sequence length="109" mass="12867" checksum="EA75EC6547E3ADF9" modified="2012-04-18" version="1">MNPNYRGYNQNTPRNYFTPSPNQRWQRNSSFDSNYSHRPNYSPQPNYSPHTPRYGTPPFRGWGNNRRSSGYHGNKPTNQRYGVEVYYNPSMTQDPWKDLPNYKPDVSAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>H2XXV2</accession>
    <name>H2XXV2_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">wild type</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01001046">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XXV2"/>
    <dbReference type="Ensembl" id="ENSCINT00000036034.1">
        <property type="protein sequence ID" value="ENSCINP00000034486.1"/>
        <property type="gene ID" value="ENSCING00000022775.1"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2653765_0_0_1"/>
    <dbReference type="InParanoid" id="H2XXV2"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000034486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="76" mass="8802" checksum="6D3F3499E82CF170" modified="2012-04-18" version="1">MHILHLTHVRTYCTSFKLFNWISNKKTQSVHSIFTIAVTPLVKKYNYSECAAGKICCFNLLCLNLHLKFGINDQKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>H2XYC6</accession>
    <name>H2XYC6_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Beta-1,4-glucuronyltransferase 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">I-beta-1,3-N-acetylglucosaminyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Poly-N-acetyllactosamine extension enzyme</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">wild type</strain>
        </source>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>3-O-[beta-D-Xyl-(1-&gt;4)-Rib-ol-P-Rib-ol-P-3-beta-D-GalNAc-(1-&gt;3)-beta-D-GlcNAc-(1-&gt;4)-(O-6-P-alpha-D-Man)]-Thr-[protein] + UDP-alpha-D-glucuronate = 3-O-[beta-D-GlcA-(1-&gt;3)-beta-D-Xyl-(1-&gt;4)-Rib-ol-P-Rib-ol-P-3-beta-D-GalNAc-(1-&gt;3)-beta-D-GlcNAc-(1-&gt;4)-(O-6-P-alpha-D-Man)]-Thr-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46860"/>
            <dbReference type="Rhea" id="RHEA-COMP:15023"/>
            <dbReference type="Rhea" id="RHEA-COMP:17482"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58052"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:142405"/>
            <dbReference type="ChEBI" id="CHEBI:177336"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyltransferase 49 family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001686">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XYC6"/>
    <dbReference type="FunCoup" id="H2XYC6">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000034660"/>
    <dbReference type="Ensembl" id="ENSCINT00000034552.1">
        <property type="protein sequence ID" value="ENSCINP00000034660.1"/>
        <property type="gene ID" value="ENSCING00000023356.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157679"/>
    <dbReference type="HOGENOM" id="CLU_019238_5_0_1"/>
    <dbReference type="InParanoid" id="H2XYC6"/>
    <dbReference type="OMA" id="CEMHIAG"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015020">
        <property type="term" value="F:glucuronosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035269">
        <property type="term" value="P:protein O-linked glycosylation via mannose"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043189">
        <property type="entry name" value="B4GAT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46420">
        <property type="entry name" value="BETA-1,4-GLUCURONYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46420:SF1">
        <property type="entry name" value="BETA-1,4-GLUCURONYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13896">
        <property type="entry name" value="Glyco_transf_49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="12" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="15" id="KW-0464">Manganese</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00030723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00032175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00032181"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00033291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00047852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000034660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="366" mass="42645" checksum="133A8E281D8B5427" modified="2012-04-18" version="1">NDVTLVTQCSVNNLYHLIETLQLWKGPISVALFVPGSSIIHAELAIKFIKQCFPLRKVQFHLVYPEHHTPTFDGNNLEIWVGLLFANDRLRCKDLFETLSSIEGENYATWGIPYPQNTLRNVAKLAVQTEYLIVLDIDVMPSYGLRENFLEKVLRNRGRNKTAETKIAYIPPAFELKINEQMPENKALLLTAWKSNQIRPFHYETCPSCHKPARYDEWKAISTNLNLGIAYEAEWQPSWEPFYIAHTSHTPDYDERFKQYGYDRISQNCELRLCGFTFVVMANSFVVHKGFKERGKFHHEKEVENKRNFNLFQNVFIPEMTKKYSSSGVDCLDSGRRKGKAKITFRQHFNKNTKEKLLRNGDQVEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>H2XZA3</accession>
    <name>H2XZA3_CIOIN</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
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    </protein>
    <organism evidence="1 2">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="H2XZA3"/>
    <dbReference type="STRING" id="7719.ENSCINP00000034987"/>
    <dbReference type="Ensembl" id="ENSCINT00000031341.1">
        <property type="protein sequence ID" value="ENSCINP00000034987.1"/>
        <property type="gene ID" value="ENSCING00000022033.1"/>
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    <dbReference type="OMA" id="HTIIITC"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026983">
        <property type="entry name" value="DHC"/>
    </dbReference>
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        <property type="entry name" value="DYNEIN HEAVY CHAIN 1, AXONEMAL-LIKE PROTEIN"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR46961:SF19">
        <property type="entry name" value="DYNEIN HEAVY CHAIN 5, AXONEMAL"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000034987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="126" mass="14548" checksum="61CC0B3DFD504FAD" modified="2012-04-18" version="1">MTWLNLVQLSRLPQFSQLLNQISRNDKGWKAWFDEEAPEEATIPEGYSSSLDTFRKLLLIRAWCPDRITAQSLHFIASTMGERYTEAVLLDYEKMLLESEKTSPMICFLTMGSDPTDNIERLARGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>H2Y0F0</accession>
    <name>H2Y0F0_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ion transport domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">wild type</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="EAAA01001458">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2Y0F0"/>
    <dbReference type="STRING" id="7719.ENSCINP00000035384"/>
    <dbReference type="Ensembl" id="ENSCINT00000034160.1">
        <property type="protein sequence ID" value="ENSCINP00000035384.1"/>
        <property type="gene ID" value="ENSCING00000024181.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161879"/>
    <dbReference type="HOGENOM" id="CLU_1192205_0_0_1"/>
    <dbReference type="InParanoid" id="H2Y0F0"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036128">
        <property type="term" value="C:CatSper complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005227">
        <property type="term" value="F:calcium-activated cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030317">
        <property type="term" value="P:flagellated sperm motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028744">
        <property type="entry name" value="CatSper4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47077:SF1">
        <property type="entry name" value="CATION CHANNEL SPERM-ASSOCIATED PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47077">
        <property type="entry name" value="ION_TRANS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
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    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="102"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="133"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="168"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="6">
        <location>
            <begin position="103"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000035384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="233" mass="27014" checksum="E4966D009C2D3D2C" modified="2012-04-18" version="1">MSRKWQAKVGSIIKTQQVSNLLSSPAANHFEEEEEEEEIQQDVSRRFCFESQEDKRIRRNRKQGLQEQLAEVEVDINEVAEKDDDDIAASVSMMVAFVLNSVYFKGMIFVVIGINAFHIALQTVETIAIPYSYIFFFVDNLIMGIFICEIVLKWYSGFVIFWKDYWNLLDFIVIFVLHLGPWLAFFSNTRLLRILRVIRAFRSLKTVTSLTGLSLVVETIMQSVTDMANIMML</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>H2Y0F6</accession>
    <accession>A0A1W3JFW3</accession>
    <name>H2Y0F6_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
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    </protein>
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        <name evidence="2" type="primary">LOC100183101</name>
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    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
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            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01001496">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009857663.1">
        <property type="nucleotide sequence ID" value="XM_009859361.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2Y0F6"/>
    <dbReference type="Ensembl" id="ENSCINT00000034293.1">
        <property type="protein sequence ID" value="ENSCINP00000035390.1"/>
        <property type="gene ID" value="ENSCING00000019311.1"/>
    </dbReference>
    <dbReference type="GeneID" id="100183101"/>
    <dbReference type="KEGG" id="cin:100183101"/>
    <dbReference type="HOGENOM" id="CLU_2385493_0_0_1"/>
    <dbReference type="InParanoid" id="H2Y0F6"/>
    <dbReference type="OrthoDB" id="5950777at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042407">
        <property type="entry name" value="NCBP2-AS2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41161">
        <property type="entry name" value="PROTEIN NCBP2AS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41161:SF1">
        <property type="entry name" value="PROTEIN NCBP2AS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="62"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="65"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000035390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="94" mass="10645" checksum="D6D2041E83595478" modified="2012-04-18" version="1">MPFRRLLGLLLNNPQLIERLSETKAIRIAAQKAVGLYNNAVAKGLSATNKLEGNIKEKVADFPKSKSSNESSFLSNFKQEMEEEYSRMKKDKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>H2Y1B2</accession>
    <name>H2Y1B2_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Ras-related protein Rab-2A</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">wild type</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Secretory vesicle</location>
            <location evidence="1">Acrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Golgi apparatus membrane</location>
            <topology evidence="13">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001782">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2Y1B2"/>
    <dbReference type="FunCoup" id="H2Y1B2">
        <property type="interactions" value="887"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000035696"/>
    <dbReference type="Ensembl" id="ENSCINT00000036515.1">
        <property type="protein sequence ID" value="ENSCINP00000035696.1"/>
        <property type="gene ID" value="ENSCING00000019877.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153886"/>
    <dbReference type="HOGENOM" id="CLU_041217_23_1_1"/>
    <dbReference type="InParanoid" id="H2Y1B2"/>
    <dbReference type="OMA" id="TNATHAC"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001669">
        <property type="term" value="C:acrosomal vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01866">
        <property type="entry name" value="Rab2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000275">
        <property type="entry name" value="Putative ras-related protein Rab-2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0636">Prenylation</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00037794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000035696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="198" mass="22561" checksum="8428A82B95A560D4" modified="2012-04-18" version="1">MSYAYLFKYIIIGDTGVGKSCLLLQFTDKRFQPVHDLTIGVEFGARMITIDGRQIKLQIWDTAGQESFRSITRSYYRGAAGALLVYDITRRDTFNHLTTWLEDARQHSSSNMVIMLIGNKSDLEARREVKKEEGEAFAREHGLIFMETSAKTAANVEEAFINTAKEIYEKIQEGVFDINNEQFLSSYAVLIHPCNSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>H2Y1V9</accession>
    <accession>A0A1W2WHM5</accession>
    <name>H2Y1V9_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Claudin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100179400</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">wild type</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell junction</location>
            <location evidence="8">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the claudin family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01000642">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002131091.1">
        <property type="nucleotide sequence ID" value="XM_002131055.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2Y1V9"/>
    <dbReference type="STRING" id="7719.ENSCINP00000035894"/>
    <dbReference type="Ensembl" id="ENSCINT00000035958.1">
        <property type="protein sequence ID" value="ENSCINP00000035894.1"/>
        <property type="gene ID" value="ENSCING00000020534.1"/>
    </dbReference>
    <dbReference type="GeneID" id="100179400"/>
    <dbReference type="KEGG" id="cin:100179400"/>
    <dbReference type="GeneTree" id="ENSGT00940000171465"/>
    <dbReference type="HOGENOM" id="CLU_076370_2_2_1"/>
    <dbReference type="InParanoid" id="H2Y1V9"/>
    <dbReference type="OMA" id="IRIQHEY"/>
    <dbReference type="OrthoDB" id="8795554at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070830">
        <property type="term" value="P:bicellular tight junction assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006187">
        <property type="entry name" value="Claudin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017974">
        <property type="entry name" value="Claudin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004031">
        <property type="entry name" value="PMP22/EMP/MP20/Claudin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12002">
        <property type="entry name" value="CLAUDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00822">
        <property type="entry name" value="PMP22_Claudin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01077">
        <property type="entry name" value="CLAUDIN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01346">
        <property type="entry name" value="CLAUDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0965">Cell junction</keyword>
    <keyword evidence="3 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0796">Tight junction</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="82"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="112"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="160"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU060637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000035894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="214" mass="23531" checksum="8081781D6F5A93CB" modified="2012-04-18" version="1">MASGLHIAVCILYVIAFILEVVTVFLPYWKRNDLEDTLNDSIVRHEGLWIRCQTFATGNWDCDDFNRFFLGLPTELQAARGFSLSSLFISFIGICLAVAATVLSVKSLQNKLTIASGGASMLAGLLIIAAVSWYANDIRIQHEYLTRLRLTGGSGTRYTFGYALFLGWVGGALQVVGSIMALCISCGNSDEEDIPPTYRYHPTKSTLAPSQEYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>H2Y2A7</accession>
    <name>H2Y2A7_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">STAT transcription factor protein interaction domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">wild type</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01001610">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2Y2A7"/>
    <dbReference type="Ensembl" id="ENSCINT00000032205.1">
        <property type="protein sequence ID" value="ENSCINP00000036042.1"/>
        <property type="gene ID" value="ENSCING00000024295.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244905"/>
    <dbReference type="HOGENOM" id="CLU_656463_0_0_1"/>
    <dbReference type="InParanoid" id="H2Y2A7"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.20">
        <property type="entry name" value="STAT transcription factor, all-alpha domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.532.10">
        <property type="entry name" value="STAT transcription factor, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001217">
        <property type="entry name" value="STAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036535">
        <property type="entry name" value="STAT_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013800">
        <property type="entry name" value="STAT_TF_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015988">
        <property type="entry name" value="STAT_TF_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013799">
        <property type="entry name" value="STAT_TF_prot_interaction"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11801">
        <property type="entry name" value="SIGNAL TRANSDUCER AND ACTIVATOR OF TRANSCRIPTION"/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
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                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1">wild type</strain>
        </source>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000036111.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    </evidence>
    <sequence length="176" mass="19304" checksum="3F145B8E4F81DDC5" modified="2012-04-18" version="1">MWPYYDIMHRLMTAQPHRQIAIVAPGLSNLKEDTSDSDVLENEFLIQNISGMSTMGGVSTIGGVSTMCSMSTQGNMVTCGLSSCSNTTTDVVTCGIIPSTITLHGRPDKTQTFQDEMEEEISSLMNTTTTTTPSSVDAHQLFFLSVAQETKNWSDLDKAKLKLEIRKLILSIQFGQ</sequence>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Wang H.G."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">wild type</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="entry name" value="TRIM-NHL_E3_ligases"/>
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        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE NHLRC1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE NHLRC1-RELATED"/>
        <property type="match status" value="1"/>
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        <location>
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    <evidence type="ECO:0000256" key="1">
        <source>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
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    <evidence type="ECO:0000313" key="6">
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>H2Y2M1</accession>
    <name>H2Y2M1_CIOIN</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">wild type</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01000201">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2Y2M1"/>
    <dbReference type="Ensembl" id="ENSCINT00000033005.1">
        <property type="protein sequence ID" value="ENSCINP00000036156.1"/>
        <property type="gene ID" value="ENSCING00000023755.1"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1197186_0_0_1"/>
    <dbReference type="InParanoid" id="H2Y2M1"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000036156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="232" mass="26581" checksum="ABFC613B87E6F855" modified="2012-04-18" version="1">MSRKVECSFVDYKALQLPCYKNDEVWMCPTVFLRQILFPNQVLKKLEDAVTKILKSSKNMTKNEVKSLREWLEYDCQQNTLSKIRIASLDVSSLSFSTTEMKEKYKTIVDEISRHSALNNVEAKRETTFQTNSNVSGKSDVQIREDNLKTGLNIEREAATPQKDFISVIDETNLMEEGQRFCLQAKDTKVSRIESNPKPCKPAPNKTLGEEFVNGYQLNQADEPTPQKTFPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>H2Y3D2</accession>
    <name>H2Y3D2_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Mediator of RNA polymerase II transcription subunit 7</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">wild type</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the Mediator complex subunit 7 family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001183">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2Y3D2"/>
    <dbReference type="STRING" id="7719.ENSCINP00000036417"/>
    <dbReference type="Ensembl" id="ENSCINT00000036721.1">
        <property type="protein sequence ID" value="ENSCINP00000036417.1"/>
        <property type="gene ID" value="ENSCING00000024109.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165241"/>
    <dbReference type="HOGENOM" id="CLU_1900889_0_0_1"/>
    <dbReference type="InParanoid" id="H2Y3D2"/>
    <dbReference type="OMA" id="CNLIDET"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037212">
        <property type="entry name" value="Med7/Med21-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009244">
        <property type="entry name" value="Mediatior_Med7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044888">
        <property type="entry name" value="Mediatior_Med7_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21428">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21428:SF11">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05983">
        <property type="entry name" value="Med7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140718">
        <property type="entry name" value="Mediator hinge subcomplex-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 6" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU364060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000036417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="133" mass="14999" checksum="A8718DD0BA17E740" modified="2012-04-18" version="1">MHHLINEYRPHQARETLKVMMALQKKNMIDVSETLCKNMDKASEILHQCSQELAAMKVKTEASTILPNNSNHSEGIDSMDIGSVLGFDSSSVIDQSNSMMEVDSGYRENSHNEEDTQDEKDIIMCSFVDGMKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>H2Y3D5</accession>
    <name>H2Y3D5_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">cysteine--tRNA ligase</fullName>
            <ecNumber evidence="3">6.1.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Cysteinyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">wild type</strain>
        </source>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">In addition to its role as an aminoacyl-tRNA synthetase, has also cysteine persulfide synthase activity. Produces reactive persulfide species such as cysteine persulfide (CysSSH) from substrate cysteine and mediate direct incorporation of CysSSH into proteins during translations, resulting in protein persulfides and polysulfides. CysSSHs behave as potent antioxidants and cellular protectants.</text>
    </comment>
    <comment type="function">
        <text evidence="12">Mitochondrial cysteine-specific aminoacyl-tRNA synthetase that catalyzes the ATP-dependent ligation of cysteine to tRNA(Cys).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>2 L-cysteine = S-sulfanyl-L-cysteine + L-alanine</text>
            <dbReference type="Rhea" id="RHEA:78543"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:58591"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:78544"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>S-disulfanyl-L-cysteine + tRNA(Cys) + ATP = (S)-disulfanyl-L-cysteinyl-tRNA(Cys) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:78651"/>
            <dbReference type="Rhea" id="RHEA-COMP:9661"/>
            <dbReference type="Rhea" id="RHEA-COMP:19120"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:229465"/>
            <dbReference type="ChEBI" id="CHEBI:229521"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:78652"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>S-sulfanyl-L-cysteine + L-cysteine = S-disulfanyl-L-cysteine + L-alanine</text>
            <dbReference type="Rhea" id="RHEA:78627"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:58591"/>
            <dbReference type="ChEBI" id="CHEBI:229465"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:78628"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>S-sulfanyl-L-cysteine + tRNA(Cys) + ATP = (S)-sulfanyl-L-cysteinyl-tRNA(Cys) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:78647"/>
            <dbReference type="Rhea" id="RHEA-COMP:9661"/>
            <dbReference type="Rhea" id="RHEA-COMP:19119"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58591"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:229520"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:78648"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>tRNA(Cys) + L-cysteine + ATP = L-cysteinyl-tRNA(Cys) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:17773"/>
            <dbReference type="Rhea" id="RHEA-COMP:9661"/>
            <dbReference type="Rhea" id="RHEA-COMP:9679"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78517"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.16"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="18">
            <dbReference type="Rhea" id="RHEA:17775"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.16" evidence="3"/>
    <dbReference type="EMBL" id="EAAA01000793">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2Y3D5"/>
    <dbReference type="FunCoup" id="H2Y3D5">
        <property type="interactions" value="169"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000036420"/>
    <dbReference type="Ensembl" id="ENSCINT00000034804.1">
        <property type="protein sequence ID" value="ENSCINP00000036420.1"/>
        <property type="gene ID" value="ENSCING00000020797.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000006347"/>
    <dbReference type="HOGENOM" id="CLU_013528_0_3_1"/>
    <dbReference type="InParanoid" id="H2Y3D5"/>
    <dbReference type="OMA" id="HAWPASE"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004817">
        <property type="term" value="F:cysteine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006423">
        <property type="term" value="P:cysteinyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00672">
        <property type="entry name" value="CysRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000027">
        <property type="entry name" value="Cysteine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1910">
        <property type="entry name" value="Cysteine-tRNA ligase, C-terminal anti-codon recognition domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00041">
        <property type="entry name" value="Cys_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015803">
        <property type="entry name" value="Cys-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024909">
        <property type="entry name" value="Cys-tRNA/MSH_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032678">
        <property type="entry name" value="tRNA-synt_1_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00435">
        <property type="entry name" value="cysS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10890:SF27">
        <property type="entry name" value="CYSTEINE--TRNA LIGASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10890">
        <property type="entry name" value="CYSTEINYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01406">
        <property type="entry name" value="tRNA-synt_1e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00983">
        <property type="entry name" value="TRNASYNTHCYS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="tRNA synthetases class I catalytic" evidence="20">
        <location>
            <begin position="33"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="506"/>
            <end position="528"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00043868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00049046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF01406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000036420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="528" mass="60525" checksum="0D81FEB80F5F5BF1" modified="2012-04-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-06-07" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1W2W8V2</accession>
    <accession>F6U3H2</accession>
    <name>A0A1W2W8V2_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PITH domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100176678</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="5">wild type</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <text evidence="1">Belongs to the PITHD1 family.</text>
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            <begin position="1"/>
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    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00025788"/>
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        <source>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="PROSITE" id="PS51532"/>
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    <sequence length="209" mass="23753" checksum="F7EE05C537E3237B" modified="2017-06-07" version="1">MCDHEDHDHGAHGHDHSHDHEDKSAHFSLYLKINLTQLECLNESEEGSGKKVFKAWDERLDRERYVESDADPELLFNIPFTGNVKLKSIALLGGENDSHPKVMKLFKNIPNMSLDQTAKEADQAFELPQSYTDVLQLPAKIARFSNINCLSIFFPECYGGEITKIYYIGLTGDFMQAQRQEVLITNYELAANPADHKQKLNNMSGHMIS</sequence>
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    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">wild type</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uS3 family.</text>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 12"/>
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        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Ribosomal_uS3m"/>
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        <property type="entry name" value="MITOCHONDRIAL 28S RIBOSOMAL PROTEIN S24"/>
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        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US3M"/>
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        <property type="entry name" value="MRP-S24"/>
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    <proteinExistence type="inferred from homology"/>
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        <source>
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        <source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000025588.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    <sequence length="163" mass="18885" checksum="14C234F525AEBD46" modified="2017-06-07" version="1">MIRNTNSLRALVPRFQHQPVCQFHASPACNKATAGRHKVSKMLNKPLTYDQAFKPNLIGVKKGWLSVHTGNLRGEQGSYQFTYEDELIRKFCVGTFYRMLPTDIVIKRRFNVIEITLLCNTVVTSKVLFLKAYSERLLSAFLKCHIKMHVFKLNNEDAIYKYI</sequence>
</entry>
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    <accession>A0A1W3JHR7</accession>
    <accession>F6UES8</accession>
    <name>A0A1W3JHR7_CIOIN</name>
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            <taxon>Ciona</taxon>
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    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
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                <person name="Doggett N."/>
                <person name="Glavina T."/>
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                <person name="Richardson P."/>
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                <person name="Kohara Y."/>
                <person name="Levine M."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_018672569.1">
        <property type="nucleotide sequence ID" value="XM_018817024.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018672570.1">
        <property type="nucleotide sequence ID" value="XM_018817025.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_026695803.1">
        <property type="nucleotide sequence ID" value="XM_026840002.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1W3JHR7"/>
    <dbReference type="Ensembl" id="ENSCINT00000005252.3">
        <property type="protein sequence ID" value="ENSCINP00000005252.3"/>
        <property type="gene ID" value="ENSCING00000002578.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100185655"/>
    <dbReference type="KEGG" id="cin:100185655"/>
    <dbReference type="InParanoid" id="A0A1W3JHR7"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000920">
        <property type="entry name" value="Myelin_P0-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13869:SF24">
        <property type="entry name" value="BASEMENT MEMBRANE-SPECIFIC HEPARAN SULFATE PROTEOGLYCAN CORE PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13869">
        <property type="entry name" value="MYELIN P0 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="459"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="560"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="568"/>
            <end position="590"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000005252.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="590" mass="65426" checksum="A3BDDBEAC153C7D8" modified="2017-06-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-06-07" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1W5BM90</accession>
    <accession>F7AAN8</accession>
    <name>A0A1W5BM90_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Prostaglandin reductase 1</fullName>
            <ecNumber evidence="4">1.3.1.48</ecNumber>
            <ecNumber evidence="5">1.3.1.74</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">15-oxoprostaglandin 13-reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Dithiolethione-inducible gene 1 protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Leukotriene B4 12-hydroxydehydrogenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">NAD(P)H-dependent alkenal/one oxidoreductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="36" type="primary">LOC100179413</name>
    </gene>
    <organism evidence="36 37">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="37" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="36" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="36" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="31">
            <text>(5S,12S)-dihydroxy-(6E,10E,12E,14Z)-eicosatetraenoate + NADP(+) = 12-oxo-(5S)-hydroxy-(6E,8E,10E,14Z)-eicosatetraenoate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:51212"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:133974"/>
            <dbReference type="ChEBI" id="CHEBI:133975"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="31">
            <dbReference type="Rhea" id="RHEA:51213"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>13,14-dihydro-15-oxo-PGF2alpha + NADP(+) = 15-oxoprostaglandin F2alpha + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50588"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:133374"/>
            <dbReference type="ChEBI" id="CHEBI:133409"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="27">
            <dbReference type="Rhea" id="RHEA:50590"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="32">
            <text>13,14-dihydro-15-oxo-prostaglandin E1 + NADP(+) = 15-oxoprostaglandin E1 + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50584"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57401"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:133408"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="32">
            <dbReference type="Rhea" id="RHEA:50586"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>13,14-dihydro-15-oxo-prostaglandin F1alpha + NADP(+) = 15-oxoprostaglandin F1alpha + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50592"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:79072"/>
            <dbReference type="ChEBI" id="CHEBI:133411"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="24">
            <dbReference type="Rhea" id="RHEA:50594"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>20-hydroxy-leukotriene B4 + NADP(+) = 12-oxo-20-hydroxy-leukotriene B4 + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:51208"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57460"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:133346"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="29">
            <dbReference type="Rhea" id="RHEA:51209"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>4-hydroxynonanal + NADP(+) = (E)-4-hydroxynon-2-enal + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:64736"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58968"/>
            <dbReference type="ChEBI" id="CHEBI:156112"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="28">
            <dbReference type="Rhea" id="RHEA:64738"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="30">
            <text>6-trans-leukotriene B4 + NADP(+) = 12-oxo-(5S)-hydroxy-(6E,8E,10E,14Z)-eicosatetraenoate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:51204"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:90723"/>
            <dbReference type="ChEBI" id="CHEBI:133974"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="30">
            <dbReference type="Rhea" id="RHEA:51205"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="33">
            <text>an n-alkanal + NADP(+) = an alk-2-enal + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13737"/>
            <dbReference type="ChEBI" id="CHEBI:12834"/>
            <dbReference type="ChEBI" id="CHEBI:13757"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.3.1.74"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="33">
            <dbReference type="Rhea" id="RHEA:13739"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>decanal + NADP(+) = (2E)-decenal + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50612"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:31457"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:133455"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="21">
            <dbReference type="Rhea" id="RHEA:50614"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>dodecanal + NADP(+) = (2E)-dodecenal + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50784"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:27836"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:133741"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="25">
            <dbReference type="Rhea" id="RHEA:50786"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="34">
            <text>hexanal + NADP(+) = (E)-hex-2-enal + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50776"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28913"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:88528"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="34">
            <dbReference type="Rhea" id="RHEA:50778"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>leukotriene B4 + NADP(+) = 12-oxo-leukotriene B4 + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50608"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57461"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:133309"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:50609"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>nonan-2-one + NADP(+) = (3E)-nonen-2-one + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50616"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:77927"/>
            <dbReference type="ChEBI" id="CHEBI:133457"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="26">
            <dbReference type="Rhea" id="RHEA:50618"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>octanal + NADP(+) = (2E)-octenal + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50780"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17935"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:61748"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="20">
            <dbReference type="Rhea" id="RHEA:50782"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>pentan-2-one + NADP(+) = (E)-pent-3-en-2-one + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50788"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16472"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:145276"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="22">
            <dbReference type="Rhea" id="RHEA:50790"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Monomer or homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NADP-dependent oxidoreductase L4BD family.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.48" evidence="4"/>
    <dbReference type="EC" id="1.3.1.74" evidence="5"/>
    <dbReference type="RefSeq" id="XP_002129777.1">
        <property type="nucleotide sequence ID" value="XM_002129741.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1W5BM90"/>
    <dbReference type="FunCoup" id="A0A1W5BM90">
        <property type="interactions" value="6"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSCINT00000004207.2">
        <property type="protein sequence ID" value="ENSCINP00000004207.2"/>
        <property type="gene ID" value="ENSCING00000002075.2"/>
    </dbReference>
    <dbReference type="GeneID" id="100179413"/>
    <dbReference type="KEGG" id="cin:100179413"/>
    <dbReference type="eggNOG" id="KOG1196">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154810"/>
    <dbReference type="InParanoid" id="A0A1W5BM90"/>
    <dbReference type="OMA" id="WMSDIPQ"/>
    <dbReference type="OrthoDB" id="809632at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047522">
        <property type="term" value="F:15-oxoprostaglandin 13-reductase [NAD(P)+] activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032440">
        <property type="term" value="F:2-alkenal reductase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006693">
        <property type="term" value="P:prostaglandin metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd08294">
        <property type="entry name" value="leukotriene_B4_DH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000121">
        <property type="entry name" value="Prostaglandin reductase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041694">
        <property type="entry name" value="ADH_N_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045010">
        <property type="entry name" value="MDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014190">
        <property type="entry name" value="PTGR1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43205">
        <property type="entry name" value="PROSTAGLANDIN REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43205:SF7">
        <property type="entry name" value="PROSTAGLANDIN REDUCTASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16884">
        <property type="entry name" value="ADH_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0007">Acetylation</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="15" id="KW-0379">Hydroxylation</keyword>
    <keyword evidence="14" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-0521">NADP</keyword>
    <keyword evidence="13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-0644">Prostaglandin metabolism</keyword>
    <keyword evidence="37" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="35">
        <location>
            <begin position="15"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00020651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00031851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00032255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00032297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00033119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00047461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00047617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00047871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00047878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00047903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00048066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00048290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00048387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00048591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00048953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00049068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00049070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="ARBA" id="ARBA00049179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="ARBA" id="ARBA00049368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="35">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="36">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000004207.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="37">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="325" mass="35390" checksum="25CE5FB1A9990575" modified="2017-06-07" version="1">MVRSKYFTKAQEFDGMPKLTDFKIIDEEIDDNLKEGEVVGEALCASVDPFIRAYPVPPGAPIIGFQVLKVTKSMNKKYPVGCKVMGFSGWRTICKLSDADVWYKVNEGQNPTVSLGALGMPGMTAYCGFLDICKPQAGETVMVNGCAGAVGNLVGQIAKIKGCKVIGCCGSDEKVKFAKSLGFDQVFNYKTCKFYPKTLKELAPDGIDCFFDNVGGQLSSDIMGCMNKGGRIAVCGAITSYNSKQPPMATMVQGLMVFKLLTMRGFLVQQYKDKFPEALVEMQQWIKEGKIKIHETPSSGFENIPRSFIDMLSGGNIGKAVVVMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>F6PKR4</accession>
    <name>F6PKR4_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">wild type</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="EAAA01001541">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6PKR4"/>
    <dbReference type="STRING" id="7719.ENSCINP00000006262"/>
    <dbReference type="Ensembl" id="ENSCINT00000006262.3">
        <property type="protein sequence ID" value="ENSCINP00000006262.3"/>
        <property type="gene ID" value="ENSCING00000003089.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000172181"/>
    <dbReference type="HOGENOM" id="CLU_145026_3_0_1"/>
    <dbReference type="InParanoid" id="F6PKR4"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000425">
        <property type="entry name" value="Myosin light chain kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47633">
        <property type="entry name" value="IMMUNOGLOBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000006262.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="101" mass="11261" checksum="535D64ED34256D8E" modified="2012-04-18" version="2">MNLNKGANFNLAMKPVTIQERGAAKFTCATVGKPEPEIAWYKDGIRIKANNKYSMRNQYGVCTLTIYDCRARDAGMYKCEANNQVGRAISEANLSVLPRLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>F6PUP9</accession>
    <name>F6PUP9_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="24">Receptor protein-tyrosine kinase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">wild type</strain>
        </source>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="F6PUP9"/>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>F6QGB1</accession>
    <name>F6QGB1_CIOIN</name>
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        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
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                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
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                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
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                <person name="Roe B.A."/>
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        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Probable subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Endoplasmic reticulum membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Golgi apparatus membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="8">The PAL motif is required for normal active site conformation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the peptidase A22A family.</text>
    </comment>
    <dbReference type="EC" id="3.4.23.-" evidence="8"/>
    <dbReference type="EMBL" id="EAAA01001313">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6RA92"/>
    <dbReference type="FunCoup" id="F6RA92">
        <property type="interactions" value="396"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000004803"/>
    <dbReference type="Ensembl" id="ENSCINT00000004803.3">
        <property type="protein sequence ID" value="ENSCINP00000004803.3"/>
        <property type="gene ID" value="ENSCING00000002361.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157923"/>
    <dbReference type="HOGENOM" id="CLU_022975_3_0_1"/>
    <dbReference type="InParanoid" id="F6RA92"/>
    <dbReference type="OMA" id="TTNLMMF"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904115">
        <property type="term" value="C:axon cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070765">
        <property type="term" value="C:gamma-secretase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042500">
        <property type="term" value="F:aspartic endopeptidase activity, intramembrane cleaving"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034205">
        <property type="term" value="P:amyloid-beta formation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019896">
        <property type="term" value="P:axonal transport of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055074">
        <property type="term" value="P:calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006509">
        <property type="term" value="P:membrane protein ectodomain proteolysis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022008">
        <property type="term" value="P:neurogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007219">
        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061053">
        <property type="term" value="P:somite development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001964">
        <property type="term" value="P:startle response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.100:FF:000001">
        <property type="entry name" value="Presenilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.100">
        <property type="entry name" value="Presenilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001108">
        <property type="entry name" value="Peptidase_A22A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006639">
        <property type="entry name" value="Preselin/SPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042524">
        <property type="entry name" value="Presenilin_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10202">
        <property type="entry name" value="PRESENILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10202:SF13">
        <property type="entry name" value="PRESENILIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01080">
        <property type="entry name" value="Presenilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01072">
        <property type="entry name" value="PRESENILIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00730">
        <property type="entry name" value="PSN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 8" id="KW-0914">Notch signaling pathway</keyword>
    <keyword evidence="8" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="46"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="128"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="158"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="183"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="207"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="372"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="399"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="320"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="320"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000004803.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="432" mass="47882" checksum="B8FD4A0BCE4D142E" modified="2012-04-18" version="2">MSNSAVSTTNANQSNNTTDSPARRIRDEAEDAEEELRYGASTVISLFIPVTLCMLVVVATITSVSYYTDQSQQQFLVYTPFHTTNAAPAQVVWESIANSLIMIGVIVVMTVFLVLLYKYRCYKVIHGWLILSTLLLVFLFAYLYLAEVLRAYNTAVDYITVVIVLWNFGVVGMICIHWKGPLLLQQAYLIIVSSLMALVFIKYLPEWTAWVILGFISIYDLLAVLCPKGPLRMLVETAQSRNESLFPALIYSSGMMWTVGMAQNAPAPNQQPRGNGSFSTLGVWVHSDNTSSTSQGSDTGFDVAWQRRHNRNSGEVIITSNETRNQARQQLGPPPEGSEEAEDKGVKLGLGDFIFYSVLVGKASATGDWNTTIACFVAILIGLCLTLILLAIYRKALPALPISITFGLVFYFSTDYVVRPYMDSINSAQVYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>F6RBK2</accession>
    <name>F6RBK2_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosome biogenesis protein WDR12 homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">wild type</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Required for maturation of ribosomal RNAs and formation of the large ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
            <location evidence="6">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
            <location evidence="6">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the WD repeat WDR12/YTM1 family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002968">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6RBK2"/>
    <dbReference type="FunCoup" id="F6RBK2">
        <property type="interactions" value="201"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000023978"/>
    <dbReference type="Ensembl" id="ENSCINT00000024224.2">
        <property type="protein sequence ID" value="ENSCINP00000023978.2"/>
        <property type="gene ID" value="ENSCING00000012967.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00930000150950"/>
    <dbReference type="HOGENOM" id="CLU_000288_57_0_1"/>
    <dbReference type="InParanoid" id="F6RBK2"/>
    <dbReference type="OMA" id="DHKYVEF"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070545">
        <property type="term" value="C:PeBoW complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043021">
        <property type="term" value="F:ribonucleoprotein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000466">
        <property type="term" value="P:maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03029">
        <property type="entry name" value="WDR12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012972">
        <property type="entry name" value="NLE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028599">
        <property type="entry name" value="WDR12/Ytm1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19855:SF11">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN WDR12"/>
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        <location>
            <begin position="342"/>
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        <source>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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        <source>
            <dbReference type="HAMAP-Rule" id="MF_03029"/>
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    </evidence>
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            <dbReference type="Pfam" id="PF08154"/>
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    </evidence>
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            <dbReference type="Ensembl" id="ENSCINP00000023978.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="10">
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            <dbReference type="Proteomes" id="UP000008144"/>
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    <sequence length="427" mass="47653" checksum="C10F40E79960E314" modified="2012-04-18" version="2">MAHFQIKLFTQQKQFSVTDNTFSVPGSSTCTELNDLVNKLLSEDKDESTRSDFDFLVNDEFIRTSLVDHVETKGLSSESVIDVEYLQRKQAPELKHSFQQPDWISSIKTTEKFIITGSYDCAVTVWDRVTTKQLVSGIGHSEPVKSIAIINYNSDEKKQKISFVSTSQDQHAVIWTYNSSTNQLSSLFCCKGHSKSVECAASNEKKSIFATGSWDSTIKVWSSSVTPEEDTQEVINSSKKKFKSGKKSLTRTAKVTLSGHTEVVSALTWLNENEICSGSWDHTIRIWDIDTAVEKSHMRGMKSILSLDYSHLSRLVVTGNTDRHVRLWDPRSGEGAVVKCNLTSHIGWVTSVHWAPNDSHTLVSGSLDNCVKMWDTRSPKAPLYDLQAHTDKVMSVDWSLPDIVASGGADCKVFTFTVNTGAVDNVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>F6REB5</accession>
    <name>F6REB5_CIOIN</name>
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        <recommendedName>
            <fullName evidence="17">VWFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">wild type</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the calcium channel subunit alpha-2/delta family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01000074">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000025169"/>
    <dbReference type="Ensembl" id="ENSCINT00000025415.2">
        <property type="protein sequence ID" value="ENSCINP00000025169.2"/>
        <property type="gene ID" value="ENSCING00000013789.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166626"/>
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    <dbReference type="InParanoid" id="F6REB5"/>
    <dbReference type="OMA" id="EIHRIHA"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005891">
        <property type="term" value="C:voltage-gated calcium channel complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005245">
        <property type="term" value="F:voltage-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Calcium voltage-gated channel auxiliary subunit alpha2delta 3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051173">
        <property type="entry name" value="Ca_channel_alpha-2/delta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013680">
        <property type="entry name" value="VDCC_a2/dsu"/>
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    <dbReference type="InterPro" id="IPR013608">
        <property type="entry name" value="VWA_N"/>
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    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="STOLID, ISOFORM H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10166">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL SUBUNIT ALPHA-2/DELTA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08473">
        <property type="entry name" value="VGCC_alpha2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08399">
        <property type="entry name" value="VWA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="4" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16" id="KW-0407">Ion channel</keyword>
    <keyword evidence="12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="10" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="domain" description="VWFA" evidence="17">
        <location>
            <begin position="199"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00007060"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000025169.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="1003" mass="115334" checksum="D335608A50E206D7" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>F6RFZ9</accession>
    <name>F6RFZ9_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">wild type</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01002972">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6RFZ9"/>
    <dbReference type="Ensembl" id="ENSCINT00000024212.2">
        <property type="protein sequence ID" value="ENSCINP00000023966.2"/>
        <property type="gene ID" value="ENSCING00000012956.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154315"/>
    <dbReference type="HOGENOM" id="CLU_1242564_0_0_1"/>
    <dbReference type="InParanoid" id="F6RFZ9"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000023966.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="223" mass="26069" checksum="D47F6CC7BC9F0D71" modified="2012-04-18" version="2">MLSVCLKCLPGAKRSTYVCTKKVLKIFCTSNRTLFLSKKNNENPTTCEAIPDPIRMRRSQCIQELCNHYVNSPDYATKSEPGVKSWPHHFITASHVLETYLLLRHHKGAKTLMHRFANIDPDRSEVPEISSFTVQHEHSLEELSFMTQFAFTLKRAGYHEVHQDIIIECLKKQHKYGNIELSVNFEDYEFARFWIYGRNKILEQTSDSEYLWKAMFSDDNVKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>F6RJV3</accession>
    <name>F6RJV3_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Electron transfer flavoprotein subunit alpha</fullName>
            <shortName evidence="9">Alpha-ETF</shortName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">wild type</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Heterodimeric electron transfer flavoprotein that accepts electrons from several mitochondrial dehydrogenases, including acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase). Required for normal mitochondrial fatty acid oxidation and normal amino acid metabolism.</text>
    </comment>
    <comment type="function">
        <text evidence="9">The electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases, including five acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9 10">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="9 10">Binds 1 FAD per dimer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Heterodimer composed of ETFA and ETFB. Identified in a complex that contains ETFA, ETFB and ETFRF1. Interaction with ETFRF1 promotes dissociation of the bound FAD and loss of electron transfer activity. Interacts with TASOR.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Heterodimer of an alpha and a beta subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the ETF alpha-subunit/FixB family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01000453">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6RJV3"/>
    <dbReference type="FunCoup" id="F6RJV3">
        <property type="interactions" value="208"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000013164"/>
    <dbReference type="Ensembl" id="ENSCINT00000013164.3">
        <property type="protein sequence ID" value="ENSCINP00000013164.3"/>
        <property type="gene ID" value="ENSCING00000006311.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000013422"/>
    <dbReference type="HOGENOM" id="CLU_034178_0_1_1"/>
    <dbReference type="InParanoid" id="F6RJV3"/>
    <dbReference type="OMA" id="WRPYAEQ"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045251">
        <property type="term" value="C:electron transfer flavoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009887">
        <property type="term" value="P:animal organ morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033539">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA dehydrogenase"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031998">
        <property type="term" value="P:regulation of fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd01715">
        <property type="entry name" value="ETF_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000041">
        <property type="entry name" value="Electron transfer flavoprotein alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1220:FF:000001">
        <property type="entry name" value="Electron transfer flavoprotein, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014730">
        <property type="entry name" value="ETF_a/b_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001308">
        <property type="entry name" value="ETF_a/FixB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033947">
        <property type="entry name" value="ETF_alpha_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014731">
        <property type="entry name" value="ETF_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018206">
        <property type="entry name" value="ETF_asu_C_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43153">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43153:SF1">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01012">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00766">
        <property type="entry name" value="ETF_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000089">
        <property type="entry name" value="Electra_flavoP_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00893">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00696">
        <property type="entry name" value="ETF_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5 9 10" id="KW-0274">FAD</keyword>
    <keyword evidence="4 9" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Electron transfer flavoprotein alpha/beta-subunit N-terminal" evidence="11">
        <location>
            <begin position="25"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="251"/>
            <end position="252"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="265"/>
            <end position="269"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="282"/>
            <end position="289"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="303"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00046201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR000089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000089-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000013164.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="334" mass="34929" checksum="5D018D7D36B01C6A" modified="2012-04-18" version="2">VEKSIIAINLPNISLLSSCQRLQSTLVVAEQSDGALTPITLNAVTAACKIGGDVSCLVAGASCSKAVEQLKSVAGVSKIIVAEDASYKGYMPESLTPLILQIQSQNNYSHILAGASAFGKNLLPRVAAKLDVDPISDIIDIKSPDTFVRTIYAGNAIQTVKTSSSVKVVTVRGTSFEAAAETGGSSSVDDAPKVEGSPLSAWVSQELSKSDRPELTAARIVISGGRGMKNGENFDMLYKLADKMNAAVGASRAAVDAGYVPNDMQVGQTGKIVAPELYIAVGISGAIQHLAGMKDSKTIVAINKDPEAPIFQVSDYGLVEDLFKAIPQMDDLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>F6RNR5</accession>
    <name>F6RNR5_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">lysophospholipase</fullName>
            <ecNumber evidence="3">3.1.1.5</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">wild type</strain>
        </source>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>1-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + H2O = sn-glycerol 3-phosphocholine + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40807"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:28610"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:40808"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>1-hexadecanoyl-sn-glycero-3-phosphate + H2O = sn-glycerol 3-phosphate + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:49092"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57518"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:49093"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>1-hexadecanoyl-sn-glycero-3-phosphocholine + H2O = sn-glycerol 3-phosphocholine + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40435"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:72998"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:40436"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a 1-acyl-sn-glycero-3-phosphocholine + H2O = sn-glycerol 3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15177"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="3.1.1.5"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:15178"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type III membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NTE family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.5" evidence="3"/>
    <dbReference type="EMBL" id="EAAA01001370">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6RNR5"/>
    <dbReference type="FunCoup" id="F6RNR5">
        <property type="interactions" value="49"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000013452"/>
    <dbReference type="Ensembl" id="ENSCINT00000013452.3">
        <property type="protein sequence ID" value="ENSCINP00000013452.3"/>
        <property type="gene ID" value="ENSCING00000006539.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000168388"/>
    <dbReference type="HOGENOM" id="CLU_000960_1_0_1"/>
    <dbReference type="InParanoid" id="F6RNR5"/>
    <dbReference type="OMA" id="CTLITCN"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004622">
        <property type="term" value="F:phosphatidylcholine lysophospholipase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd07225">
        <property type="entry name" value="Pat_PNPLA6_PNPLA7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000010">
        <property type="entry name" value="neuropathy target esterase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1090.10:FF:000001">
        <property type="entry name" value="neuropathy target esterase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000510">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050301">
        <property type="entry name" value="NTE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056556">
        <property type="entry name" value="NTE1_P-loop_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14226:SF29">
        <property type="entry name" value="NEUROPATHY TARGET ESTERASE SWS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14226">
        <property type="entry name" value="NEUROPATHY TARGET ESTERASE/SWISS CHEESE D.MELANOGASTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24179">
        <property type="entry name" value="NTE_Ploop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 16" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="10 16" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="17">
        <location>
            <begin position="60"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="17">
        <location>
            <begin position="172"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="PNPLA" evidence="18">
        <location>
            <begin position="520"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXGXXG" evidence="16">
        <location>
            <begin position="524"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXSXG" evidence="16">
        <location>
            <begin position="551"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DGA/G" evidence="16">
        <location>
            <begin position="673"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="16">
        <location>
            <position position="553"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="16">
        <location>
            <position position="673"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000013452.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="947" mass="106855" checksum="0F2AFB173CA70811" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>F6RQJ6</accession>
    <name>F6RQJ6_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
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                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
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    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Chapman J."/>
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                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
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                <person name="Meinertzhagen I.A."/>
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                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="25" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
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                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
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                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="18">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="F6S801"/>
    <dbReference type="STRING" id="7719.ENSCINP00000026946"/>
    <dbReference type="Ensembl" id="ENSCINT00000027192.2">
        <property type="protein sequence ID" value="ENSCINP00000026946.2"/>
        <property type="gene ID" value="ENSCING00000015059.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160615"/>
    <dbReference type="HOGENOM" id="CLU_270507_0_0_1"/>
    <dbReference type="InParanoid" id="F6S801"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005112">
        <property type="term" value="F:Notch binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0007219">
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000146">
        <property type="entry name" value="Putative neurogenic locus notch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000472">
        <property type="entry name" value="Uncharacterized protein, isoform A"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.740.10:FF:000001">
        <property type="entry name" value="vitamin K-dependent protein S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.740.10">
        <property type="entry name" value="Coagulation Factor IX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017857">
        <property type="entry name" value="Coagulation_fac-like_Gla_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001774">
        <property type="entry name" value="DSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035972">
        <property type="entry name" value="GLA-like_dom_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000294">
        <property type="entry name" value="GLA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051022">
        <property type="entry name" value="Notch_Cell-Fate_Det"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24049">
        <property type="entry name" value="CRUMBS FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24049:SF22">
        <property type="entry name" value="DROSOPHILA CRUMBS HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00594">
        <property type="entry name" value="Gla"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00001">
        <property type="entry name" value="GLABLOOD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00051">
        <property type="entry name" value="DSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SMART" id="SM00069">
        <property type="entry name" value="GLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57630">
        <property type="entry name" value="GLA-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="GLA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50998">
        <property type="entry name" value="GLA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="10" id="KW-0221">Differentiation</keyword>
    <keyword evidence="16 18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 18" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="4" id="KW-0301">Gamma-carboxyglutamic acid</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15 20" id="KW-0472">Membrane</keyword>
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    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="8 21" id="KW-0732">Signal</keyword>
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    <keyword evidence="14 20" id="KW-1133">Transmembrane helix</keyword>
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            <end position="23"/>
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    <feature type="chain" id="PRO_5003341339" description="Delta-like protein" evidence="21">
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            <begin position="24"/>
            <end position="1203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="1033"/>
            <end position="1054"/>
        </location>
    </feature>
    <feature type="domain" description="Gla" evidence="23">
        <location>
            <begin position="225"/>
            <end position="271"/>
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    </feature>
    <feature type="domain" description="EGF-like" evidence="22">
        <location>
            <begin position="271"/>
            <end position="309"/>
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    </feature>
    <feature type="domain" description="EGF-like" evidence="22">
        <location>
            <begin position="311"/>
            <end position="348"/>
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    </feature>
    <feature type="domain" description="EGF-like" evidence="22">
        <location>
            <begin position="350"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="22">
        <location>
            <begin position="388"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="22">
        <location>
            <begin position="426"/>
            <end position="463"/>
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    </feature>
    <feature type="domain" description="EGF-like" evidence="22">
        <location>
            <begin position="466"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="22">
        <location>
            <begin position="520"/>
            <end position="556"/>
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    </feature>
    <feature type="domain" description="EGF-like" evidence="22">
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            <begin position="566"/>
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    </feature>
    <feature type="domain" description="EGF-like" evidence="22">
        <location>
            <begin position="604"/>
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    </feature>
    <feature type="domain" description="EGF-like" evidence="22">
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    <feature type="domain" description="EGF-like" evidence="22">
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            <end position="716"/>
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    </feature>
    <feature type="domain" description="EGF-like" evidence="22">
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            <begin position="718"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="22">
        <location>
            <begin position="782"/>
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        </location>
    </feature>
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        <location>
            <begin position="1178"/>
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        </location>
    </feature>
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        <location>
            <begin position="1178"/>
            <end position="1190"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="1191"/>
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        </location>
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        <location>
            <begin position="280"/>
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    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="299"/>
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    <feature type="disulfide bond" evidence="18">
        <location>
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    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="376"/>
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    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="414"/>
            <end position="423"/>
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    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="453"/>
            <end position="462"/>
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    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="508"/>
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    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="546"/>
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    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
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            <end position="601"/>
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    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="630"/>
            <end position="639"/>
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    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="668"/>
            <end position="677"/>
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    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="706"/>
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    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="749"/>
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    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="808"/>
            <end position="817"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
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        <source>
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    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
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    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022976"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
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    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
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    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000026946.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="26">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1203" mass="134396" checksum="C1CEB548BA57F4F9" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>F6SLY8</accession>
    <name>F6SLY8_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
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                <person name="Larimer F."/>
                <person name="Detter C."/>
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                <person name="Glavina T."/>
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                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
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            <strain evidence="2">wild type</strain>
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    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <dbReference type="EMBL" id="EAAA01001611">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="F6SLY8"/>
    <dbReference type="Ensembl" id="ENSCINT00000022619.2">
        <property type="protein sequence ID" value="ENSCINP00000022373.2"/>
        <property type="gene ID" value="ENSCING00000011792.2"/>
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            <begin position="1"/>
            <end position="24"/>
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        <location>
            <begin position="25"/>
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        <source>
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<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>F6SSG7</accession>
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        <recommendedName>
            <fullName evidence="19">Cellular tumor antigen p53</fullName>
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    <organism evidence="16 17">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
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    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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                <person name="Inaba K."/>
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            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="16">wild type</strain>
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    </reference>
    <reference evidence="18" key="3">
        <citation type="journal article" date="2016" name="Protein Sci." volume="25" first="410" last="422">
            <title>Structural investigations of the p53/p73 homologs from the tunicate species Ciona intestinalis reveal the sequence requirements for the formation of a tetramerization domain.</title>
            <authorList>
                <person name="Heering J."/>
                <person name="Jonker H.R."/>
                <person name="Lohr F."/>
                <person name="Schwalbe H."/>
                <person name="Dotsch V."/>
            </authorList>
            <dbReference type="PubMed" id="26473758"/>
            <dbReference type="DOI" id="10.1002/pro.2830"/>
        </citation>
        <scope>STRUCTURE BY NMR OF 374-419</scope>
    </reference>
    <reference evidence="16" key="4">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="5">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="11">Binds 1 zinc ion per subunit.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the p53 family.</text>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="PDB" id="2MW4">
        <property type="method" value="NMR"/>
        <property type="chains" value="A/B/C/D=374-419"/>
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    <dbReference type="PDBsum" id="2MW4"/>
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        <property type="protein sequence ID" value="ENSCINP00000004447.2"/>
        <property type="gene ID" value="ENSCING00000002175.2"/>
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    <dbReference type="GeneTree" id="ENSGT00950000183153"/>
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    <dbReference type="InParanoid" id="F6SSG7"/>
    <dbReference type="OMA" id="HKKGEPC"/>
    <dbReference type="EvolutionaryTrace" id="F6SSG7"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 3"/>
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    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:1990841">
        <property type="term" value="F:promoter-specific chromatin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
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        <property type="project" value="GO_Central"/>
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        <property type="entry name" value="p53/RUNT-type_TF_DNA-bd_sf"/>
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        <property type="entry name" value="p53_DNA-bd"/>
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        <property type="entry name" value="p53_tumour_suppressor"/>
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        <property type="entry name" value="P53 PROTEIN LONG FORM VARIANT 1"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00870">
        <property type="entry name" value="P53"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="P53_tetramer"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="P53SUPPRESSR"/>
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        <property type="entry name" value="p53 tetramerization domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
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    <keyword evidence="8" id="KW-0010">Activator</keyword>
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    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="p53 DNA-binding" evidence="14">
        <location>
            <begin position="141"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="p53 tetramerisation" evidence="15">
        <location>
            <begin position="375"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="332"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="221"/>
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        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="283"/>
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        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="287"/>
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        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
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    <feature type="site" description="Interaction with DNA" evidence="12">
        <location>
            <position position="162"/>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
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        <source>
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        <source>
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        <source>
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        <source>
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    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0008006" key="19">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="419" mass="47437" checksum="F9648AC704FC01D3" modified="2011-07-27" version="1">MAVADTSELNFPDSQESFSDFWMNTLSENNELPSWQTDLNQEYDQCKETVDVLQLDTTKANDIEFPVSEFLTSSQASQQSIGDLFAQSLPSTQCGGNSQVTTVSKHEYPDSGYVMTLPNNSVLAELQTSSLQTEVSLLPNNEYPGIYNFEINFGEKTESAPKSAPFTYSYSLQKLFVKMNENCPIKFRCSPQPPSGCVIRAIPVFEKPNNVTEIVTRCFNHRNECRTESSDSNTPNSHLIRVESKSNNIQYCLTHEGRECVVVPYEPPHSGSEYMALLYRFMCLSSCRTETGINRRPLLTIFNLESETGELLGKRVVSTRICACPGRDRTQEEEKKNVTSQNKSRKRLCKSATNSKSIPVVQKNEENKVDNDDDGDVVYTLNIRGKRKFEKVKEYKEALDLLDYVQPDVKKACCQRNQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
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    <accession>A0A1W2WHY5</accession>
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            <fullName evidence="2">Coiled-coil domain-containing protein 169-like</fullName>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
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            <taxon>Phlebobranchia</taxon>
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            <taxon>Ciona</taxon>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01001303">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002131441.1">
        <property type="nucleotide sequence ID" value="XM_002131405.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6SVR6"/>
    <dbReference type="Ensembl" id="ENSCINT00000014931.2">
        <property type="protein sequence ID" value="ENSCINP00000014931.2"/>
        <property type="gene ID" value="ENSCING00000007276.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100180557"/>
    <dbReference type="KEGG" id="cin:100180557"/>
    <dbReference type="GeneTree" id="ENSGT00940000165436"/>
    <dbReference type="HOGENOM" id="CLU_083726_1_0_1"/>
    <dbReference type="InParanoid" id="F6SVR6"/>
    <dbReference type="OrthoDB" id="6615663at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028022">
        <property type="entry name" value="DUF4600"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28671">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 169"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28671:SF3">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 169"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15372">
        <property type="entry name" value="DUF4600"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="10"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="107"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000014931.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="214" mass="25534" checksum="6669C00D7F0D5894" modified="2011-07-27" version="1">MERGMEDYSLEEMEAELEHERQMKKMLDQSLLGLKITVEDLEKKYQQIDEEGNEWKTRYETQQEMNTQLELQIKDLKEKLKPNHRTNSKNRKDQVPVSVKNMEGLSESGLKGLIKQMEKERSSLQNQLRDLEWRLDQESKALYKASEGRKKYLTELADAEFRLDSYMKLQAKKQAQKENNGINMHKRILPPLPKRVSVNKRNIQENRIPRRFQN</sequence>
</entry>
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    <accession>F6T7Y2</accession>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
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    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
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        <lineage>
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    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01000350">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6T7Y2"/>
    <dbReference type="STRING" id="7719.ENSCINP00000015492"/>
    <dbReference type="Ensembl" id="ENSCINT00000015492.3">
        <property type="protein sequence ID" value="ENSCINP00000015492.3"/>
        <property type="gene ID" value="ENSCING00000007562.3"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1980820_0_0_1"/>
    <dbReference type="InParanoid" id="F6T7Y2"/>
    <dbReference type="OMA" id="HAACLFI"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003342246" evidence="2">
        <location>
            <begin position="22"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000015492.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="126" mass="13903" checksum="0D8CEAFD38563A2F" modified="2012-04-18" version="2">MYASVGSILPLLLFGNFLAIALPKESLEAALGVTGAEALAKYTSPVGTLWTLIPSNYHNHAACLFIFLTLLLLLAARFAARSMPTLSRKRKRSLVERKSYVQNHVKTKKAALYKEYLQQSVAAHDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>F6TDV1</accession>
    <accession>A0A1W2WJL3</accession>
    <name>F6TDV1_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Serum response factor-binding protein 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC100183338</name>
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    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
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            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
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    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_002130181.1">
        <property type="nucleotide sequence ID" value="XM_002130145.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6TDV1"/>
    <dbReference type="STRING" id="7719.ENSCINP00000001689"/>
    <dbReference type="Ensembl" id="ENSCINT00000001689.3">
        <property type="protein sequence ID" value="ENSCINP00000001689.3"/>
        <property type="gene ID" value="ENSCING00000000933.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100183338"/>
    <dbReference type="KEGG" id="cin:100183338"/>
    <dbReference type="GeneTree" id="ENSGT00390000006478"/>
    <dbReference type="HOGENOM" id="CLU_962955_0_0_1"/>
    <dbReference type="InParanoid" id="F6TDV1"/>
    <dbReference type="OMA" id="SKLLEIX"/>
    <dbReference type="OrthoDB" id="3364872at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037393">
        <property type="entry name" value="Bud22/SRFB1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23325">
        <property type="entry name" value="SERUM RESPONSE FACTOR-BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23325:SF1">
        <property type="entry name" value="SERUM RESPONSE FACTOR-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="221"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="242"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000001689.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="289" mass="33184" checksum="004884000C4FCF3C" modified="2012-04-18" version="2">MDFNNEMILMRKDLKRAKVHVISKLVRRNKSLKSKNAPEHLKQKNISRANRLLQEIEIIKDMKPDVIFKHLVTNDRSGNLDEMALRVLSRIGTISFIEPKISQLKLLLHDFEANRKKEEVSLNAESAVLEKSSELPWRQTNVVKQPETNQNILPHPEKCKTVLSLSEKVFENLGEKFLDENEIVDDLSQDPFFKGEDTLVSGSCATTLSEDISKSLLKVKKDRKRNRPGQNARDKLYGKTNSSKKGKPKKVKTKESVSLHPSWQASQVKKKENIIQKFQGTKIRFSDSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>F6THC9</accession>
    <name>F6THC9_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Propionyl-CoA carboxylase beta chain, mitochondrial</fullName>
            <ecNumber evidence="3">6.4.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Propanoyl-CoA:carbon dioxide ligase subunit beta</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">wild type</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>butanoyl-CoA + hydrogencarbonate + ATP = (2S)-ethylmalonyl-CoA + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:59520"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57371"/>
            <dbReference type="ChEBI" id="CHEBI:60909"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:59521"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>propanoyl-CoA + hydrogencarbonate + ATP = (S)-methylmalonyl-CoA + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23720"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57327"/>
            <dbReference type="ChEBI" id="CHEBI:57392"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.4.1.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:23721"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Metabolic intermediate metabolism; propanoyl-CoA degradation; succinyl-CoA from propanoyl-CoA: step 1/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">The holoenzyme is a dodecamer composed of 6 PCCA/alpha subunits and 6 PCCB/beta subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AccD/PCCB family.</text>
    </comment>
    <dbReference type="EC" id="6.4.1.3" evidence="3"/>
    <dbReference type="EMBL" id="EAAA01002404">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6THC9"/>
    <dbReference type="FunCoup" id="F6THC9">
        <property type="interactions" value="182"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000016122"/>
    <dbReference type="Ensembl" id="ENSCINT00000016122.3">
        <property type="protein sequence ID" value="ENSCINP00000016122.3"/>
        <property type="gene ID" value="ENSCING00000007866.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157741"/>
    <dbReference type="HOGENOM" id="CLU_018822_6_0_1"/>
    <dbReference type="InParanoid" id="F6THC9"/>
    <dbReference type="OMA" id="ENTSYMF"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004658">
        <property type="term" value="F:propionyl-CoA carboxylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009062">
        <property type="term" value="P:fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000017">
        <property type="entry name" value="Propionyl-CoA carboxylase subunit beta 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000016">
        <property type="entry name" value="Propionyl-CoA carboxylase, beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051047">
        <property type="entry name" value="AccD/PCCB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034733">
        <property type="entry name" value="AcCoA_carboxyl_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011763">
        <property type="entry name" value="COA_CT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011762">
        <property type="entry name" value="COA_CT_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43842">
        <property type="entry name" value="PROPIONYL-COA CARBOXYLASE BETA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43842:SF2">
        <property type="entry name" value="PROPIONYL-COA CARBOXYLASE BETA CHAIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01039">
        <property type="entry name" value="Carboxyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50989">
        <property type="entry name" value="COA_CT_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50980">
        <property type="entry name" value="COA_CT_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CoA carboxyltransferase N-terminal" evidence="9">
        <location>
            <begin position="29"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="CoA carboxyltransferase C-terminal" evidence="10">
        <location>
            <begin position="291"/>
            <end position="531"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00041138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00042797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000016122.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="537" mass="58659" checksum="692D5C64DF9AC663" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>F6TX05</accession>
    <name>F6TX05_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Ueno K."/>
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                <person name="Matsumoto J."/>
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                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01001435">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="ENSCING00000011512.2"/>
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        <property type="component" value="Chromosome 2"/>
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        <property type="entry name" value="AB_hydrolase_fold"/>
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        <property type="entry name" value="CarbesteraseB"/>
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        <property type="entry name" value="ACETYLCHOLINESTERASE 1-LIKE"/>
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        <property type="entry name" value="CARBOXYLIC ESTER HYDROLASE"/>
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        <property type="entry name" value="COesterase"/>
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        <property type="entry name" value="alpha/beta-Hydrolases"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carboxylesterase type B" evidence="1">
        <location>
            <begin position="23"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000021961.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="251" mass="27939" checksum="6D5500E2B11122C2" modified="2012-04-18" version="2">GEIVSNAYIALAGTRPGLDYDYSDILSQELTDLVFACPNRALGRFASVSSTAAKVSGQAPLYLYNSAYPFGGEACIEWNSVDGVCGYAFHSSEIIFVFKSPEGYGVTFTDNDDLASDIFSTYWSRFAVDGNPNSNLRPQLAEVDYWPPYSSENNWNNILIDIPKSVVQTDYREQFCDMLDATGFYINLTGPYQPRSVLSQPVLSQKFQRKISLQDHGLPEPYNGAGSAIYPSFFRVLILPLIYFASQFCAY</sequence>
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    <accession>F6X6I9</accession>
    <name>F6X6I9_CIOIN</name>
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        <recommendedName>
            <fullName evidence="6">Fibrinogen C-terminal domain-containing protein</fullName>
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    <organism evidence="7 8">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">wild type</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01002226">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6X6I9"/>
    <dbReference type="Ensembl" id="ENSCINT00000023036.2">
        <property type="protein sequence ID" value="ENSCINP00000022790.2"/>
        <property type="gene ID" value="ENSCING00000012104.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163443"/>
    <dbReference type="HOGENOM" id="CLU_066147_0_0_1"/>
    <dbReference type="InParanoid" id="F6X6I9"/>
    <dbReference type="OMA" id="NYVASSC"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070492">
        <property type="term" value="F:oligosaccharide binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.215.10">
        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040941">
        <property type="entry name" value="GGGWT_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16146">
        <property type="entry name" value="INTELECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16146:SF46">
        <property type="entry name" value="INTELECTIN-1A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0430">Lectin</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="6">
        <location>
            <begin position="43"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="88"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="92"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000022790.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="303" mass="33944" checksum="E8AF5F604523C030" modified="2012-04-18" version="2">EVGYNLCLNSIPYHAIATATNKWDSQQYLEFNSSCNDLRGIEHKDKVYMITDSDGLGYPVYCDMTTNGGGWTLVASIHENDINGKCTTGDRWSSEHGNSAEHPHGDGNWENNNTFGDVTHAASDDYKNAAYFQLNGSDVMIWHVPNNTLLQRYKSSSYFRYRTNNGFLSKQGGNLQTLFQKFPLKDNKYPKGDNGPSSSVVFDKGSKKNTCNFGTSTQGEVEPGYIQFRTINYEQSAFALCLGVKYRTNNYNTEHACVGGTSYHDGPSDTNLYCGDYSALEFPISGWNATQQLKTSVFMVFYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
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    <name>F6X972_CIOIN</name>
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        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
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        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ciona</taxon>
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    <reference evidence="7" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="F6XSX0"/>
    <dbReference type="STRING" id="7719.ENSCINP00000003968"/>
    <dbReference type="Ensembl" id="ENSCINT00000003968.3">
        <property type="protein sequence ID" value="ENSCINP00000003968.3"/>
        <property type="gene ID" value="ENSCING00000017940.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155894"/>
    <dbReference type="HOGENOM" id="CLU_1524593_0_0_1"/>
    <dbReference type="InParanoid" id="F6XSX0"/>
    <dbReference type="OMA" id="PARCIMQ"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016316">
        <property type="term" value="F:phosphatidylinositol-3,4-bisphosphate 4-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039034">
        <property type="entry name" value="INPP4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12187">
        <property type="entry name" value="AGAP000124-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12187:SF11">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-3,4-BISPHOSPHATE 4-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000003968.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="176" mass="19757" checksum="B4FD24A681E7EBCC" modified="2012-04-18" version="2">MQEFNNSINEKALGKMYNYYKRFRAFHLDRSKLPRDTEARAVLVDRLSRPLNALDEIMTLLESNVKPRHGKPHLATSGAGVLTLVAEFCNRLGGCHVIMCNNGVHRSTMACCLEQSLILARCHGLPPRQLNSAAFQFSHMGARTYCGIKNPETRDKTVKSAPRLFRPTTLETVSTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>F6Y8I1</accession>
    <name>F6Y8I1_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the IAP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="F6Y8I1"/>
    <dbReference type="STRING" id="7719.ENSCINP00000021657"/>
    <dbReference type="Ensembl" id="ENSCINT00000021903.2">
        <property type="protein sequence ID" value="ENSCINP00000021657.2"/>
        <property type="gene ID" value="ENSCING00000011291.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164164"/>
    <dbReference type="HOGENOM" id="CLU_016347_2_0_1"/>
    <dbReference type="InParanoid" id="F6Y8I1"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00022">
        <property type="entry name" value="BIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1170.10:FF:000002">
        <property type="entry name" value="Baculoviral IAP repeat containing 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1170.10">
        <property type="entry name" value="Inhibitor Of Apoptosis Protein (2mihbC-IAP-1), Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001370">
        <property type="entry name" value="BIR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050784">
        <property type="entry name" value="IAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10044:SF139">
        <property type="entry name" value="DEATH-ASSOCIATED INHIBITOR OF APOPTOSIS 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10044">
        <property type="entry name" value="INHIBITOR OF APOPTOSIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00653">
        <property type="entry name" value="BIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00238">
        <property type="entry name" value="BIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57924">
        <property type="entry name" value="Inhibitor of apoptosis (IAP) repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50143">
        <property type="entry name" value="BIR_REPEAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="7">
        <location>
            <begin position="215"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="146"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="147"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000021657.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="262" mass="29462" checksum="3CB1D70632C2D60A" modified="2012-04-18" version="2">YFFTGPGDLVKCWYCGNKLKNFDVEDEPWMEHAKWFPQCEFLLQNKGHAFVANIQTSYPRNVHVVNLPSVESGPTFLPSPIQNHETLATQQRQLLEEGMTQDYVQQAVEIFGLVAVERVMKRLIESSGCYYQTSEGLINAVLESSETTTTTTTTTNTQDTSTPTQSGHYFFPVDSDVRIPVQRTNFIQPSIPMHGTDNSDNISSGQSNSNSQLCCKVCLNRDATIAFNPCGHLCVCQSCSPRLNACPICRRSIQQKIRIYFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>F6YIX5</accession>
    <name>F6YIX5_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex</fullName>
            <ecNumber evidence="4">2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>(R)-lipoate</name>
            <dbReference type="ChEBI" id="CHEBI:83088"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the 2-oxoacid dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="F6YIX5"/>
    <dbReference type="FunCoup" id="F6YIX5">
        <property type="interactions" value="177"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000003336"/>
    <dbReference type="Ensembl" id="ENSCINT00000003336.3">
        <property type="protein sequence ID" value="ENSCINP00000003336.3"/>
        <property type="gene ID" value="ENSCING00000001641.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156046"/>
    <dbReference type="InParanoid" id="F6YIX5"/>
    <dbReference type="OMA" id="TIKQKPW"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045254">
        <property type="term" value="C:pyruvate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006086">
        <property type="term" value="P:pyruvate decarboxylation to acetyl-CoA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06849">
        <property type="entry name" value="lipoyl_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000010">
        <property type="entry name" value="Acetyltransferase component of pyruvate dehydrogenase complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.10:FF:000003">
        <property type="entry name" value="Acetyltransferase component of pyruvate dehydrogenase complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.320.10">
        <property type="entry name" value="E3-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003016">
        <property type="entry name" value="2-oxoA_DH_lipoyl-BS"/>
    </dbReference>
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    <dbReference type="PANTHER" id="PTHR23151:SF90">
        <property type="entry name" value="DIHYDROLIPOYLLYSINE-RESIDUE ACETYLTRANSFERASE COMPONENT OF PYRUVATE DEHYDROGENASE COMPLEX, MITOCHONDRIAL-RELATED"/>
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    <dbReference type="Pfam" id="PF00198">
        <property type="entry name" value="2-oxoacid_dh"/>
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    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="5">
        <location>
            <begin position="29"/>
            <end position="105"/>
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    </feature>
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        <location>
            <begin position="158"/>
            <end position="195"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00007317"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022823"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
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    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU003423"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50968"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51826"/>
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    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000003336.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="476" mass="51708" checksum="848A43077C3434B4" modified="2012-04-18" version="2">MAAVRILRICFNKFNFSRTIYTKSSCCSPIQIQMPALSPTMEEGTITKWLISEGDAVEIGDAMCEVETDKAVVTMEANEDGTLAKILIPDGTRGVKINSPIAILAEEGEDLLEASKFDPPPISFHPPTSSVEEVVTETSQIHATNTPNILELGKMGDKISPAVRQMLNQFNIEVTNIHGTGPKGIRLKGDVIKYIAQKGLNPVHQHVSTPTKQVTTPPTKATEVKKDSVATKPPVQVHEGDYEDLDLSSVRKVIAKRLTESKQTIPHAYSTIDCSINKVLDLRRQLAKDGVKVSLNDFIIKCVASTLRRVPEVNVVWRGHETKHSDTIDISIAVATDGGLITPIITGADRKGLSAISEEIRELASKARSGKLQPHEYQGGSFTISNLGMFGVKEFTAVINPPQSCIMAVGGTRVRPASSPVDLDDDVISDVTESATDSVMTVTMSSDARVVDDELASKFLSTFKQNMENPLYMGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F6YJH9</accession>
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            <fullName evidence="2">Uncharacterized protein</fullName>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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            <taxon>Eukaryota</taxon>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="F6YJH9"/>
    <dbReference type="Ensembl" id="ENSCINT00000022736.2">
        <property type="protein sequence ID" value="ENSCINP00000022490.2"/>
        <property type="gene ID" value="ENSCING00000011879.2"/>
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        <property type="component" value="Chromosome 7"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="169"/>
        </location>
    </feature>
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            <begin position="8"/>
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        </location>
    </feature>
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            <begin position="40"/>
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        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="145"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000022490.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="169" mass="17690" checksum="46058621DF8C71DD" modified="2012-04-18" version="2">MGCGGSKASTAAVAATTAAKHAPPPPTNGTTEITATVKPTLREEPPKDSSPEPIKKTTEEAPVKSEAEYTDANGTERERSATPKQDEPAAPEPAEPVAEVETREKTPTQETKPDEATAKPAEITSDEKPAEEQAAEEQKPEEPAEAPVESNEPEQEAAAPPAEEQAAES</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>F6YKX8</accession>
    <accession>A0A1W2WJ45</accession>
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        <recommendedName>
            <fullName evidence="5">60S ribosomal protein L3-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100177118</name>
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    <organism evidence="7 8">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
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                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
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                <person name="Doyle S."/>
                <person name="Francino P."/>
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                <person name="Haga S."/>
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                <person name="Imai K.S."/>
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                <person name="Makabe K.W."/>
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                <person name="Medina M."/>
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                <person name="Mount S."/>
                <person name="Morishita T."/>
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                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
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            </authorList>
            <dbReference type="PubMed" id="18854010"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">wild type</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Heart- and skeletal muscle-specific component of the ribosome, which regulates muscle function. Component of the large ribosomal subunit in striated muscle cells: replaces the RPL3 paralog in the ribosome in these cells. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. Inhibits myotube growth and muscle function.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL3 family.</text>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="Ensembl" id="ENSCINT00000013831.3">
        <property type="protein sequence ID" value="ENSCINP00000013831.3"/>
        <property type="gene ID" value="ENSCING00000006731.3"/>
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    <dbReference type="GeneID" id="100177118"/>
    <dbReference type="KEGG" id="cin:100177118"/>
    <dbReference type="GeneTree" id="ENSGT00390000017606"/>
    <dbReference type="HOGENOM" id="CLU_033361_2_1_1"/>
    <dbReference type="InParanoid" id="F6YKX8"/>
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    <dbReference type="OrthoDB" id="1611972at2759"/>
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        <location>
            <begin position="1"/>
            <end position="36"/>
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    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="18"/>
            <end position="31"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006540"/>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00055008"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="Ensembl" id="ENSCINP00000013831.3"/>
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            <dbReference type="Proteomes" id="UP000008144"/>
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    <sequence length="411" mass="46477" checksum="F7B00C5CB82B18BF" modified="2012-04-18" version="2">MSHRKFSAPRHGSLGFLPRKRSSRHRGKVKAFPRDDPSKPCHLTAFISYKAGMTHVVREVDRPGSKVNKKEVVDAVTILEAPPMIVVGVIGYIETPRGLRTLKTLFAEHLSDECKRRFYKNWYACKKKAFTKSCRKWQDEEGKRQIDRDFNQMKKYCKVIRVIAHTQMKLLPITQKKSHIMEIQVNGGTIPEKVDYARDLLEKPVPVSNIFAKDEMIDIIGVNKGHGEKGVTSRWHTKKLPRKTHKGLRKVACIGAWHPARVAYSVARSGQKGYHHRTEVNKKIYRLGAGIHKEGGKLIKNNAATETDITDKSITPMGGFPHYGEVKNDFVMIKGCCIGPKKRVLTLRKSLITHTKRAALEKINLKWIDTSSKFGHGRFQTHEEKRAFMGPLKKDRLAAAAAAATTTTTTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>F6YPI9</accession>
    <name>F6YPI9_CIOIN</name>
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        <recommendedName>
            <fullName evidence="8">Transporter</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">wild type</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002875">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="ENSCINP00000011403.3"/>
        <property type="gene ID" value="ENSCING00000005514.3"/>
    </dbReference>
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        <property type="component" value="Chromosome 9"/>
    </dbReference>
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        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="F:gamma-aminobutyric acid:sodium:chloride symporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd11496">
        <property type="entry name" value="SLC6sbd-TauT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000175">
        <property type="entry name" value="Na/ntran_symport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037272">
        <property type="entry name" value="SNS_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF037979">
        <property type="entry name" value="Na_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SODIUM/CHLORIDE DEPENDENT TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616:SF289">
        <property type="entry name" value="TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00209">
        <property type="entry name" value="SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00176">
        <property type="entry name" value="NANEUSMPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161070">
        <property type="entry name" value="SNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00610">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00754">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50267">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0915">Sodium</keyword>
    <keyword evidence="8" id="KW-0769">Symport</keyword>
    <keyword evidence="3 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="16"/>
            <end position="34"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="46"/>
            <end position="67"/>
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    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="88"/>
            <end position="115"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="179"/>
            <end position="198"/>
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    <feature type="transmembrane region" description="Helical" evidence="9">
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            <begin position="210"/>
            <end position="231"/>
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    <feature type="transmembrane region" description="Helical" evidence="9">
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            <begin position="251"/>
            <end position="268"/>
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    <feature type="transmembrane region" description="Helical" evidence="9">
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            <begin position="289"/>
            <end position="315"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="9">
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            <begin position="348"/>
            <end position="373"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="394"/>
            <end position="421"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="9">
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            <begin position="427"/>
            <end position="446"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="9">
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            <begin position="467"/>
            <end position="490"/>
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    <feature type="transmembrane region" description="Helical" evidence="9">
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            <begin position="502"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
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        <location>
            <position position="25"/>
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        <ligand>
            <name>Na(+)</name>
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        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
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        <location>
            <position position="264"/>
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        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
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        <ligand>
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        <location>
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        <ligand>
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        <source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU003732"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>F6Z1X8</accession>
    <accession>A0A1W2WEH0</accession>
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        <submittedName>
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        <name evidence="10" type="primary">LOC100180823</name>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Tunicata</taxon>
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            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
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    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">wild type</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
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    <comment type="similarity">
        <text evidence="2 9">Belongs to the cytochrome P450 family.</text>
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        <property type="project" value="InterPro"/>
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        <ligandPart>
            <name>Fe</name>
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    </evidence>
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        <source>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
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                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <location>
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        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
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        <location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
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        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000011134.3"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>F6ZF56</accession>
    <name>F6ZF56_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Nucleoporin Nup54 alpha-helical domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">wild type</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the NUP54 family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001481">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6ZF56"/>
    <dbReference type="FunCoup" id="F6ZF56">
        <property type="interactions" value="1328"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000017696"/>
    <dbReference type="Ensembl" id="ENSCINT00000017696.3">
        <property type="protein sequence ID" value="ENSCINP00000017696.3"/>
        <property type="gene ID" value="ENSCING00000008671.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165029"/>
    <dbReference type="HOGENOM" id="CLU_033371_0_0_1"/>
    <dbReference type="InParanoid" id="F6ZF56"/>
    <dbReference type="OMA" id="MMQTRLH"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044613">
        <property type="term" value="C:nuclear pore central transport channel"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006607">
        <property type="term" value="P:NLS-bearing protein import into nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006999">
        <property type="term" value="P:nuclear pore organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036228">
        <property type="term" value="P:protein localization to nuclear inner membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.490:FF:000003">
        <property type="entry name" value="nucleoporin p54 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.490">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024864">
        <property type="entry name" value="Nup54/Nup57/Nup44"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025712">
        <property type="entry name" value="Nup54_alpha-helical_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040985">
        <property type="entry name" value="Nup54_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13000">
        <property type="entry name" value="NUCLEOPORIN P54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13000:SF0">
        <property type="entry name" value="NUCLEOPORIN P54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13874">
        <property type="entry name" value="Nup54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18437">
        <property type="entry name" value="Nup54_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="7" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0811">Translocation</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Nucleoporin Nup54 alpha-helical" evidence="10">
        <location>
            <begin position="316"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="domain" description="Nup54 C-terminal interacting" evidence="11">
        <location>
            <begin position="468"/>
            <end position="505"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00060798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF13874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF18437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000017696.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="521" mass="57277" checksum="B810A2A6E1989CF8" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>F6ZIX6</accession>
    <name>F6ZIX6_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Arsenite methyltransferase</fullName>
            <ecNumber evidence="4">2.1.1.137</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100181209</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>arsenic triglutathione + 2 [thioredoxin]-dithiol + 2 S-adenosyl-L-methionine + H2O = dimethylarsinous acid + 2 [thioredoxin]-disulfide + 3 glutathione + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:69464"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:23808"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:183640"/>
            <dbReference type="EC" id="2.1.1.137"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>arsenic triglutathione + 3 [thioredoxin]-dithiol + 3 S-adenosyl-L-methionine = trimethylarsine + 3 [thioredoxin]-disulfide + 3 glutathione + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:69432"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:27130"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:183640"/>
            <dbReference type="EC" id="2.1.1.137"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>arsenic triglutathione + [thioredoxin]-dithiol + S-adenosyl-L-methionine + 2 H2O = methylarsonous acid + [thioredoxin]-disulfide + 3 glutathione + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:69460"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17826"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:183640"/>
            <dbReference type="EC" id="2.1.1.137"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the methyltransferase superfamily. Arsenite methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.137" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="F6ZIX6"/>
    <dbReference type="STRING" id="7719.ENSCINP00000027827"/>
    <dbReference type="Ensembl" id="ENSCINT00000028073.2">
        <property type="protein sequence ID" value="ENSCINP00000027827.2"/>
        <property type="gene ID" value="ENSCING00000015865.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000001742"/>
    <dbReference type="HOGENOM" id="CLU_052868_0_1_1"/>
    <dbReference type="InParanoid" id="F6ZIX6"/>
    <dbReference type="OMA" id="FVMAYIE"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030791">
        <property type="term" value="F:arsenite methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018872">
        <property type="term" value="P:arsonoacetate metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009404">
        <property type="term" value="P:toxin metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.5.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026669">
        <property type="entry name" value="Arsenite_MeTrfase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025714">
        <property type="entry name" value="Methyltranfer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43675">
        <property type="entry name" value="ARSENITE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43675:SF8">
        <property type="entry name" value="ARSENITE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13847">
        <property type="entry name" value="Methyltransf_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase" evidence="9">
        <location>
            <begin position="71"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00034487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00034521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00034545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000027827.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="343" mass="38521" checksum="AC3516ABB62AF360" modified="2012-04-18" version="2">MSNCVGTIASVKKYYGQTLRSTCDLKSSLSSSVCEALRPPPHIKRAITMVHDDVASRYFGCGLAIPFDQLEGVKILDLGCGSGRDCYVMSQLVGEDGYVTGVDMTKEQLDIARKYVDHHREKFGFKKSNVKFVEGFVEDMKEIEDSSIDIAISNCVVNLCEDKESVYKEVYRVLKPGGEFYFSDIYADSDVTEAAKNNEVLWGEGMGGALNWRTFHKLTSSVGFECPRIMTSFPVQMKHPEECINIITGDVKYVSVLYRMFKLDSDVIKTPCNVTYKGTIKYNQDELKFDLHLTFHTDDITTIDGITAATLRTSRFFQHFKFSEPVANGPEPVGLENPFDVIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>F6ZJ76</accession>
    <name>F6ZJ76_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Zinc finger protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">zf(c2h2)-11</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="F6ZJ76"/>
    <dbReference type="STRING" id="7719.ENSCINP00000015848"/>
    <dbReference type="Ensembl" id="ENSCINT00000015848.3">
        <property type="protein sequence ID" value="ENSCINP00000015848.3"/>
        <property type="gene ID" value="ENSCING00000007722.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286939"/>
    <dbReference type="HOGENOM" id="CLU_541386_0_0_1"/>
    <dbReference type="InParanoid" id="F6ZJ76"/>
    <dbReference type="OMA" id="HESKHIG"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000478">
        <property type="entry name" value="Zinc finger protein 133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000688">
        <property type="entry name" value="zinc finger protein 197 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24408:SF58">
        <property type="entry name" value="TRANSCRIPTION FACTOR (TFIIIA), PUTATIVE (AFU_ORTHOLOGUE AFUA_1G05150)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24408">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="9">
        <location>
            <begin position="24"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="213"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="243"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="272"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="300"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="328"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="357"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="386"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="414"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="136"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="140"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000015848.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="504" mass="56843" checksum="E707EF07C5348BCB" modified="2012-04-18" version="2">MAQTLIAKRILSYINEMRMNKRFCDVEFITRCGKVFAAHSCVLTCVSPCFETWILNGNYDKETRVTKVLLPESVHASTFSVLLDLIYTCNVKVRKLNNCNQNSLDELTLNVNVELEETASELSLSFDENVLKNINMSSDKTSHESKNTESRQRKSIRLSDPSDDLLLESVEPDEVSLEFDVGSLHNEVTASASNEVQKLHNDGKQNVTAGKKVSCDICSRLFTNINGLERHKKRVHHGKTSPIQCSECKKLFKSKTKLSKHQLTPCLGKQKSQCYICKKTFASKYIMGLHLKIHTGDELFKCHVCNKKFPYKSSLTNHLILHNHQNYFLCSVCGKSFSQKVRAKEHESKHIGVKSSLSCSECNSKFTQKSALARHVSSVHNQHRPYVCETCGRCFARKNKLLEHTRTHTGEKPYVCSLCGSSYKANRSLKIHKQRRHGIKDLVVDQELSPKTATVTILSSPPGVVRSELTSIATLDLMSPQTLDVNKPCQTLKDNPHSLQGELN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>F6ZN98</accession>
    <name>F6ZN98_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
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                <person name="Rigoutsos I."/>
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                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
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                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
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                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="5">wild type</strain>
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        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Chapman J."/>
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                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
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                <person name="Saiga H."/>
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                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
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                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
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                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
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                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
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                <person name="Rigoutsos I."/>
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                <person name="Shoguchi E."/>
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                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">wild type</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the malectin family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01000735">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002127786.1">
        <property type="nucleotide sequence ID" value="XM_002127750.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6ZSN0"/>
    <dbReference type="FunCoup" id="F6ZSN0">
        <property type="interactions" value="28"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000019885"/>
    <dbReference type="Ensembl" id="ENSCINT00000019885.3">
        <property type="protein sequence ID" value="ENSCINP00000019885.3"/>
        <property type="gene ID" value="ENSCING00000009792.4"/>
    </dbReference>
    <dbReference type="GeneID" id="100183899"/>
    <dbReference type="KEGG" id="cin:100183899"/>
    <dbReference type="GeneTree" id="ENSGT00390000016504"/>
    <dbReference type="HOGENOM" id="CLU_065446_1_0_1"/>
    <dbReference type="InParanoid" id="F6ZSN0"/>
    <dbReference type="OMA" id="PNPYSMD"/>
    <dbReference type="OrthoDB" id="10013439at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.430">
        <property type="entry name" value="Galactose-binding lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021720">
        <property type="entry name" value="Malectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039155">
        <property type="entry name" value="MLEC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13460:SF0">
        <property type="entry name" value="MALECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11721">
        <property type="entry name" value="Malectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0732">Signal</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014090579" evidence="12">
        <location>
            <begin position="21"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="260"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="Malectin" evidence="13">
        <location>
            <begin position="23"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="194"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="194"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="204"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF11721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000019885.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="281" mass="31073" checksum="F56D39784121BD16" modified="2012-04-18" version="2">MAVDILLLLTLICTISSVSASEVVYAVNCGGDSHVDSFGINYLGDPLQVGTKSDYGRRQTVTRAPPSDNILYQTERWHDSTFSYNIPVQKDGDYVLVLKFAEVYFYGPNLKVFNVQLNDHSIVQDLDIFNLVGHSTAYDEIIPLSIKKGKLSVTGEFSTFNGVLKLDFMKGAADNPKICAFYLMKGTVADVPKLPELKHRPTSQDEETGEEEEPVEDDESEDTEDQPEQRKTSKTQSSRKSKLKSGPPAADPYAEDSGSLVMPIMVAFGLFIPTVLCLCRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>F6ZTT3</accession>
    <name>F6ZTT3_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Smoothened</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor Fz/Smo family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="F6ZTT3"/>
    <dbReference type="STRING" id="7719.ENSCINP00000002708"/>
    <dbReference type="Ensembl" id="ENSCINT00000002708.3">
        <property type="protein sequence ID" value="ENSCINP00000002708.3"/>
        <property type="gene ID" value="ENSCING00000001387.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157206"/>
    <dbReference type="HOGENOM" id="CLU_007873_3_1_1"/>
    <dbReference type="InParanoid" id="F6ZTT3"/>
    <dbReference type="OMA" id="WYEGIEG"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005113">
        <property type="term" value="F:patched binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007417">
        <property type="term" value="P:central nervous system development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071679">
        <property type="term" value="P:commissural neuron axon guidance"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007389">
        <property type="term" value="P:pattern specification process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007224">
        <property type="term" value="P:smoothened signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07451">
        <property type="entry name" value="CRD_SMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.2000.10">
        <property type="entry name" value="Frizzled cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015526">
        <property type="entry name" value="Frizzled/SFRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000539">
        <property type="entry name" value="Frizzled/Smoothened_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020067">
        <property type="entry name" value="Frizzled_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036790">
        <property type="entry name" value="Frizzled_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041771">
        <property type="entry name" value="SMO_CRD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11309">
        <property type="entry name" value="FRIZZLED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11309:SF35">
        <property type="entry name" value="PROTEIN SMOOTHENED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01534">
        <property type="entry name" value="Frizzled"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01392">
        <property type="entry name" value="Fz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00489">
        <property type="entry name" value="FRIZZLED"/>
    </dbReference>
    <dbReference type="SMART" id="SM00063">
        <property type="entry name" value="FRI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01330">
        <property type="entry name" value="Frizzled"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63501">
        <property type="entry name" value="Frizzled cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50038">
        <property type="entry name" value="FZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="192"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="228"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="278"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="325"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="364"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="413"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="domain" description="FZ" evidence="11">
        <location>
            <begin position="26"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="12">
        <location>
            <begin position="192"/>
            <end position="441"/>
        </location>
    </feature>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
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                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
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                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
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                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
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    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000015811.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="203" mass="22555" checksum="6D80BFB9CC5D6239" modified="2011-07-27" version="1">MDFVITSVQQVTDGNQNQSLPGDITVSTENEFRSNTPQESWSFSSCFPKCGINSRYFCTCGLGVALLLAGSCCLILYFVQSRLLLIPALALFIVGIVVSSIGEWYCRIKIRHRQVQARRERERISDEDCERVAQAVQRQIAAQAAANDQPPSYFTLAALPTVSSLSNDAYVTDEEEAPPYTAYDVTKDVLPSYSEATNKLHKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>F7ALF0</accession>
    <accession>A0A1W2W0G4</accession>
    <name>F7ALF0_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Caltractin-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100186461</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_002120146.1">
        <property type="nucleotide sequence ID" value="XM_002120110.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F7ALF0"/>
    <dbReference type="STRING" id="7719.ENSCINP00000022975"/>
    <dbReference type="Ensembl" id="ENSCINT00000023221.2">
        <property type="protein sequence ID" value="ENSCINP00000022975.2"/>
        <property type="gene ID" value="ENSCING00000012260.2"/>
    </dbReference>
    <dbReference type="GeneID" id="100186461"/>
    <dbReference type="KEGG" id="cin:100186461"/>
    <dbReference type="GeneTree" id="ENSGT00940000173312"/>
    <dbReference type="HOGENOM" id="CLU_108639_0_0_1"/>
    <dbReference type="InParanoid" id="F7ALF0"/>
    <dbReference type="OMA" id="KGFSMFD"/>
    <dbReference type="OrthoDB" id="343296at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000003">
        <property type="entry name" value="Calmodulin A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050230">
        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF59">
        <property type="entry name" value="EF-HAND SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="72"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="108"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="182"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="9"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000022975.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="217" mass="24699" checksum="6585814F0A489BE3" modified="2012-04-18" version="2">MPSKKKRKSISEDKKAKAPDFQYPTEAIPSNIPDIPPPGQALLTVLQSNEVNEKQFLGLKLPTKIFEQLTIQEIRDLKVVFDAFDLDNSSTIDIKELHRAMKILGFQVSRNEVRDMMADVDTQNRGEISFADFLLFIIERQSDSRDILEEIRHGFKMFVDPDEGLVTATSLKKVCRDAGVKFNDKEIANMMEVADTNGDEVIDEDEFLAIMLKTNLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>F7AQJ2</accession>
    <name>F7AQJ2_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">wild type</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001360">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F7AQJ2"/>
    <dbReference type="STRING" id="7719.ENSCINP00000022127"/>
    <dbReference type="Ensembl" id="ENSCINT00000022373.2">
        <property type="protein sequence ID" value="ENSCINP00000022127.2"/>
        <property type="gene ID" value="ENSCING00000011618.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160615"/>
    <dbReference type="HOGENOM" id="CLU_004826_12_3_1"/>
    <dbReference type="InParanoid" id="F7AQJ2"/>
    <dbReference type="OMA" id="HGGTCNL"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000012">
        <property type="entry name" value="Delta-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000066">
        <property type="entry name" value="FAT atypical cadherin 4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000321">
        <property type="entry name" value="Protein delta homolog 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000057">
        <property type="entry name" value="protocadherin Fat 1 isoform X2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000255">
        <property type="entry name" value="Sushi, nidogen and EGF-like domains 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013032">
        <property type="entry name" value="EGF-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051830">
        <property type="entry name" value="NOTCH_homolog"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24033">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24033:SF193">
        <property type="entry name" value="NETRIN-G1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12661">
        <property type="entry name" value="hEGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
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</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    <dbReference type="Ensembl" id="ENSCINT00000022361.1">
        <property type="protein sequence ID" value="ENSCINP00000022115.1"/>
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        </location>
    </feature>
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        <location>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
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        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000022115.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="84" mass="9213" checksum="0CB3AFD172313C4A" modified="2011-07-27" version="1">VASNDCADNVDGNNDEPESSYIDVVSICEKSPHITHTEHDNEPMEKSNDSPRGEDSAKESLPQAIRAVHPSKTCGELSDHVLRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>F7AUQ0</accession>
    <name>F7AUQ0_CIOIN</name>
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            <fullName evidence="16">Sodium channel protein</fullName>
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    <organism evidence="20 21">
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        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
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    <reference evidence="21" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
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                <person name="Haga S."/>
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                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
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                <person name="Nomoto H."/>
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                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
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                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
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                <person name="Levine M."/>
                <person name="Satoh N."/>
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            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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            <dbReference type="PubMed" id="18854010"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">wild type</strain>
        </source>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 16">Cell membrane</location>
            <topology evidence="1 16">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the sodium channel (TC 1.A.1.10) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01000378">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="EAAA01000379">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000011383"/>
    <dbReference type="Ensembl" id="ENSCINT00000011383.3">
        <property type="protein sequence ID" value="ENSCINP00000011383.3"/>
        <property type="gene ID" value="ENSCING00000005490.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000167131"/>
    <dbReference type="HOGENOM" id="CLU_000540_5_0_1"/>
    <dbReference type="InParanoid" id="F7AUQ0"/>
    <dbReference type="OMA" id="GEEDMKH"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001518">
        <property type="term" value="C:voltage-gated sodium channel complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005248">
        <property type="term" value="F:voltage-gated sodium channel activity"/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>IDENTIFICATION</scope>
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                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">wild type</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="EAAA01000589">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001239016.1">
        <property type="nucleotide sequence ID" value="NM_001252087.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XL41"/>
    <dbReference type="Ensembl" id="ENSCINT00000036931.1">
        <property type="protein sequence ID" value="ENSCINP00000030373.1"/>
        <property type="gene ID" value="ENSCING00000022164.1"/>
    </dbReference>
    <dbReference type="GeneID" id="100179524"/>
    <dbReference type="KEGG" id="cin:100179524"/>
    <dbReference type="GeneTree" id="ENSGT00390000002043"/>
    <dbReference type="HOGENOM" id="CLU_990305_0_0_1"/>
    <dbReference type="InParanoid" id="H2XL41"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 10"/>
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    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="Cell_adhesion_signaling"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11640:SF164">
        <property type="entry name" value="MAM DOMAIN-CONTAINING GLYCOSYLPHOSPHATIDYLINOSITOL ANCHOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11640">
        <property type="entry name" value="NEPHRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5010392985" description="Ig-like domain-containing protein" evidence="6">
        <location>
            <begin position="20"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="7">
        <location>
            <begin position="38"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="7">
        <location>
            <begin position="154"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
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        </source>
    </evidence>
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            <dbReference type="Ensembl" id="ENSCINP00000030373.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="281" mass="31549" checksum="7B04D5C02D986CAD" modified="2012-04-18" version="1">MKCLVILFAAFATVVVTTASENRIQVKTIFNEVTARYEGDDVELYCEYSIASGVDLPVTVTWKVGDGPRYASSIYECTGYTNSAKTCNTEQVFDPAYEGRIKFEQNYLTLSSVQGSDNRNYWCQVALEGSSQHSDEEFDMKSVVLVVPDSGTMPKFNTVIVQEDSVQQLGKEMLLTCECLQSSIYCEDIYWYKGPVDSENSNYDLIYSRVTQHYASSPGTYIQYQSYGDWEHRAVLSGDSLYVTMLKASDAGRYWCEGRTAAYKSIGEWGTDAHSTVVEIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>H2XL64</accession>
    <name>H2XL64_CIOIN</name>
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        <recommendedName>
            <fullName evidence="14">RecF/RecN/SMC N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">wild type</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SMC family. SMC6 subfamily.</text>
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    <dbReference type="EMBL" id="EAAA01000862">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="H2XL64"/>
    <dbReference type="FunCoup" id="H2XL64">
        <property type="interactions" value="300"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000030396"/>
    <dbReference type="Ensembl" id="ENSCINT00000035783.1">
        <property type="protein sequence ID" value="ENSCINP00000030396.1"/>
        <property type="gene ID" value="ENSCING00000021570.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00550000074816"/>
    <dbReference type="HOGENOM" id="CLU_009063_0_1_1"/>
    <dbReference type="InParanoid" id="H2XL64"/>
    <dbReference type="OMA" id="MCHDHFY"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030915">
        <property type="term" value="C:Smc5-Smc6 complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="P-loop_NTPase"/>
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        <property type="entry name" value="RecF/RecN/SMC_N"/>
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    <dbReference type="PANTHER" id="PTHR19306">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES 5,6 SMC5, SMC6"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR19306:SF6">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 6"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
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    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="3"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="644"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="328"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="644"/>
            <end position="659"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023054"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02463"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000030396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="1036" mass="119546" checksum="29E1CBA790E3E81F" modified="2012-04-18" version="1"> </sequence>
</entry>
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    <accession>H2XL95</accession>
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        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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            <taxon>Metazoa</taxon>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="H2XL95"/>
    <dbReference type="Ensembl" id="ENSCINT00000034998.1">
        <property type="protein sequence ID" value="ENSCINP00000030427.1"/>
        <property type="gene ID" value="ENSCING00000019482.1"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2866976_0_0_1"/>
    <dbReference type="InParanoid" id="H2XL95"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000030427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="64" mass="7349" checksum="7CD3852AE190DBAF" modified="2012-04-18" version="1">MKVTCFSIVWSENDSRCNTGVLFHTPRANYEFPNKKHCDLILHCLLLDKFPLGSCVTSYPWGNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>H2XLA2</accession>
    <name>H2XLA2_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">26S proteasome non-ATPase regulatory subunit 9</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">26S proteasome regulatory subunit p27</fullName>
        </alternativeName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
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            <taxon>Phlebobranchia</taxon>
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            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Acts as a chaperone during the assembly of the 26S proteasome, specifically of the base subcomplex of the PA700/19S regulatory complex (RC). During the base subcomplex assembly is part of an intermediate PSMD9:PSMC6:PSMC3 module, also known as modulator trimer complex; PSMD9 is released during the further base assembly process.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Interacts with PSMC3. Part of a transient complex (modulator) containing PSMD9, PSMC6 and PSMC3 formed during the assembly of the 26S proteasome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the proteasome subunit p27 family.</text>
    </comment>
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    <dbReference type="FunCoup" id="H2XLA2">
        <property type="interactions" value="734"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000030434"/>
    <dbReference type="Ensembl" id="ENSCINT00000035915.1">
        <property type="protein sequence ID" value="ENSCINP00000030434.1"/>
        <property type="gene ID" value="ENSCING00000022473.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000004147"/>
    <dbReference type="HOGENOM" id="CLU_073146_2_1_1"/>
    <dbReference type="InParanoid" id="H2XLA2"/>
    <dbReference type="OMA" id="DWGGRGM"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="project" value="GO_Central"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PDZ_6"/>
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        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035269">
        <property type="entry name" value="PSMD9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12651">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12651:SF1">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 9"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nas2 N-terminal" evidence="8">
        <location>
            <begin position="21"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="7">
        <location>
            <begin position="127"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00054581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00062195"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF18265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000030434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="206" mass="22840" checksum="A393D281BC33C26C" modified="2012-04-18" version="1">RSKTQNSNSGKSETTSMAEYLKDLIRKKDKLESDIQSWFDVLKSQGNVGMMTSLVDAGGFPRSDVDVVQVRTARHNIVCLQNDHRDVMEQIKRGLEEYHSQIGRDHVVQVQQPTPEVMEGFATLDLVSRDSPAEEAGLKVGDVIIEFGSVTSRNFQSIREIGEVVKHSVGRSLNIIVKRGETKLNILLTPHSWGGRGLLGCNVVAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>H2XMR4</accession>
    <name>H2XMR4_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
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            <taxon>Phlebobranchia</taxon>
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            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">wild type</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    <sequence length="39" mass="4574" checksum="741C413A1006B3C8" modified="2012-04-18" version="1">MHMERSIVGITFAHFIKLEQSIETYYNRVVFGNPTSSNR</sequence>
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        <name type="scientific">Ciona intestinalis</name>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
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                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
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                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
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                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
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                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="protein sequence ID" value="ENSCINP00000031553.1"/>
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        <location>
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        <location>
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            <begin position="9"/>
            <end position="25"/>
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            <begin position="27"/>
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            <begin position="78"/>
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            <begin position="92"/>
            <end position="101"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000031553.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    <sequence length="197" mass="21504" checksum="555F488F0C9B8F83" modified="2012-04-18" version="1">MDNLDDVLGDLLGELEGLTQQLEDGPPTKPTQPSHPPPPPNKPIANKPPPQIPHKEDIDLNSMLDDLCLFEENLASQTIEDTNKPSNTGRDSLPRDFDDGGQKFNTLKRAGQGEKSFTHGGFVNYHDDNEDISLEGLTNDIEQEQSKQPGITEEELATLSPVLLELDGLVNSLCQSPPATPIPATYPSNKYTPPMTP</sequence>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
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            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
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    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
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                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
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                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
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                <person name="Doggett N."/>
                <person name="Glavina T."/>
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                <person name="Richardson P."/>
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                <person name="Kohara Y."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <reaction evidence="17 23">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 23">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
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    <dbReference type="AlphaFoldDB" id="H2XPX4"/>
    <dbReference type="STRING" id="7719.ENSCINP00000031708"/>
    <dbReference type="Ensembl" id="ENSCINT00000035912.1">
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        <property type="gene ID" value="ENSCING00000018308.1"/>
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    <dbReference type="GeneTree" id="ENSGT00940000163130"/>
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    <dbReference type="InParanoid" id="H2XPX4"/>
    <dbReference type="OMA" id="CFERICN"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
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    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Kinase-like_dom_sf"/>
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        <property type="entry name" value="Prot_kinase_dom"/>
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    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
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    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
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    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
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        <property type="entry name" value="TESTIS-SPECIFIC SERINE_THREONINE-PROTEIN KINASE 1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 18 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="11" id="KW-0221">Differentiation</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 18 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 19" id="KW-0723">Serine/threonine-protein kinase</keyword>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
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                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
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                <person name="Richardson P."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Wasserscheid J."/>
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                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
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                <person name="Zeller R.W."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>H2XQP5</accession>
    <name>H2XQP5_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="H2XQP5"/>
    <dbReference type="Ensembl" id="ENSCINT00000032207.1">
        <property type="protein sequence ID" value="ENSCINP00000031979.1"/>
        <property type="gene ID" value="ENSCING00000018607.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162538"/>
    <dbReference type="HOGENOM" id="CLU_153566_0_0_1"/>
    <dbReference type="InParanoid" id="H2XQP5"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24064">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 22 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="46"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="79"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="106"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="1"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000031979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="139" mass="15255" checksum="CEE001B95A857AEC" modified="2012-04-18" version="1">MLVGSFLFGSTADRFGRKPTIIATFIGTLGCMVGVTFSTTVEMYTVFRTGTALFLAGASIGSFVYVIEIVPQKWVGAFGLMYQGIFTCGYMNLSALAFIWRDWHEIMGVATILSAPYLIIALVIPESPRWQFTNGKDKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>H2XQZ3</accession>
    <name>H2XQZ3_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="H2XQZ3"/>
    <dbReference type="Ensembl" id="ENSCINT00000030501.1">
        <property type="protein sequence ID" value="ENSCINP00000032077.1"/>
        <property type="gene ID" value="ENSCING00000019362.1"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3241870_0_0_1"/>
    <dbReference type="InParanoid" id="H2XQZ3"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000032077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="43" mass="5313" checksum="237078E5E978F65C" modified="2012-04-18" version="1">MFDPFIHYMQNNNVIKHLKFIYCDINIKTNKTNKIFPKFHVTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>H2XR83</accession>
    <name>H2XR83_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized LOC104266776</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC104266776</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="H2XR83"/>
    <dbReference type="Ensembl" id="ENSCINT00000035581.1">
        <property type="protein sequence ID" value="ENSCINP00000032167.1"/>
        <property type="gene ID" value="ENSCING00000019634.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01140000286787"/>
    <dbReference type="HOGENOM" id="CLU_2849007_0_0_1"/>
    <dbReference type="InParanoid" id="H2XR83"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003578122" evidence="1">
        <location>
            <begin position="23"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000032167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
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                <person name="Tassy O."/>
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                <person name="Zhang C."/>
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                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
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                <person name="Richardson P."/>
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                <person name="Ogasawara M."/>
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                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
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                <person name="Lindquist E."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="De Tomaso A."/>
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                <person name="Hastings K.E."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
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                <person name="Wiley G.B."/>
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                <person name="Hastings K.E."/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
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                <person name="Campbell R.K."/>
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                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
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                <person name="Huang W."/>
                <person name="Kawashima T."/>
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                <person name="Martinez D."/>
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                <person name="Wang H.G."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Yamada L."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Ogasawara M."/>
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                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
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                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
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                <person name="Lindquist E."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
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        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003112">
        <property type="entry name" value="Olfac-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050605">
        <property type="entry name" value="Olfactomedin-like_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23192:SF85">
        <property type="entry name" value="GLIOMEDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23192">
        <property type="entry name" value="OLFACTOMEDIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02191">
        <property type="entry name" value="OLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51132">
        <property type="entry name" value="OLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Olfactomedin-like" evidence="4">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000034152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="121" mass="14147" checksum="8BA6164C884DD2C7" modified="2012-04-18" version="1">MVGKLNPVTLRMVRPVIRTNIQKREVANAFIICQVLYTVSSYSDTNAYISYSYDLNRRIKQRLKIPFKNLYKKNVMIDYNPRNRYIYAWDDGYQVAYKSTVDQSGGRLVNGRIIAYNGTFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>H2XWY7</accession>
    <name>H2XWY7_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Myosin-7B</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Myosin cardiac muscle beta chain</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Myosin heavy chain 7B, cardiac muscle beta isoform</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="primary">LOC100176037</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">wild type</strain>
        </source>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Involved in muscle contraction.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Myofibril</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 19">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01000727">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000034171"/>
    <dbReference type="Ensembl" id="ENSCINT00000031161.1">
        <property type="protein sequence ID" value="ENSCINP00000034171.1"/>
        <property type="gene ID" value="ENSCING00000023791.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160705"/>
    <dbReference type="HOGENOM" id="CLU_000192_8_0_1"/>
    <dbReference type="InParanoid" id="H2XWY7"/>
    <dbReference type="OMA" id="MNEYRGK"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030016">
        <property type="term" value="C:myofibril"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032982">
        <property type="term" value="C:myosin filament"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01377">
        <property type="entry name" value="MYSc_class_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.820:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000003">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000006">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000013">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000008">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.4820:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.530:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.360:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000019">
        <property type="entry name" value="Myosin heavy chain, isoform G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000010">
        <property type="entry name" value="Myosin heavy chain, isoform G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000024">
        <property type="entry name" value="Myosin heavy chain, isoform J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.720:FF:000001">
        <property type="entry name" value="Myosin heavy chain, muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.370">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.360">
        <property type="entry name" value="Myosin S1 fragment, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008989">
        <property type="entry name" value="Myosin_S1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002928">
        <property type="entry name" value="Myosin_tail"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004009">
        <property type="entry name" value="SH3_Myosin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014751">
        <property type="entry name" value="XRCC4-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45615:SF27">
        <property type="entry name" value="MYOSIN HEAVY CHAIN, MUSCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45615">
        <property type="entry name" value="MYOSIN HEAVY CHAIN, NON-MUSCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02736">
        <property type="entry name" value="Myosin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01576">
        <property type="entry name" value="Myosin_tail_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90257">
        <property type="entry name" value="Myosin rod fragments"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51844">
        <property type="entry name" value="SH3_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 19" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="7 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 20" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 19" id="KW-0505">Motor protein</keyword>
    <keyword evidence="12" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="9 19" id="KW-0518">Myosin</keyword>
    <keyword evidence="6 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0787">Thick filament</keyword>
    <feature type="domain" description="Myosin N-terminal SH3-like" evidence="23">
        <location>
            <begin position="32"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="Myosin motor" evidence="22">
        <location>
            <begin position="86"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1577"/>
            <end position="1612"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1901"/>
            <end position="1927"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="20">
        <location>
            <begin position="840"/>
            <end position="1156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="1591"/>
            <end position="1602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="1603"/>
            <end position="1612"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <begin position="179"/>
            <end position="186"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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    <dbReference type="EMBL" id="EAAA01000233">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XYQ6"/>
    <dbReference type="Ensembl" id="ENSCINT00000035028.1">
        <property type="protein sequence ID" value="ENSCINP00000034790.1"/>
        <property type="gene ID" value="ENSCING00000023592.1"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3073822_0_0_1"/>
    <dbReference type="InParanoid" id="H2XYQ6"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="27"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000034790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="53" mass="5992" checksum="91855EB4FA1BF61E" modified="2012-04-18" version="1">MQCIEMEPVYQRQTEDNGSSKEPKKPFFKILCVILVLLICGIAILLSIIALIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>H2XZV0</accession>
    <name>H2XZV0_CIOIN</name>
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        <recommendedName>
            <fullName evidence="1">Aldehyde dehydrogenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
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            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01002402">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XZV0"/>
    <dbReference type="STRING" id="7719.ENSCINP00000035184"/>
    <dbReference type="Ensembl" id="ENSCINT00000034401.1">
        <property type="protein sequence ID" value="ENSCINP00000035184.1"/>
        <property type="gene ID" value="ENSCING00000021180.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158815"/>
    <dbReference type="HOGENOM" id="CLU_005391_1_4_1"/>
    <dbReference type="InParanoid" id="H2XZV0"/>
    <dbReference type="OMA" id="ETESIMW"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016620">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="ALdehyde deHydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Aldehyde dehydrogenase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11699">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="1">
        <location>
            <begin position="3"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000035184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="173" mass="18847" checksum="1EACCAC2AC3D46A3" modified="2012-04-18" version="1">MKKIGDPMDVDTDHGPQINKYQQDRILAMIDQGVKDGCTLLCGGKKVEGLKGHYVQPTVMVGMSDHMEIAKEEIFGPVQLIFKFKTIAEVIERSNASQYGLVAGVFTNNINNAMTVARCARVGTVWVNCFFALNANAPFGGCKQSGSGRDLGEEALDAYTEVKTVYIKTPPLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>H2Y1F2</accession>
    <name>H2Y1F2_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Repulsive guidance molecule C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
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            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
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            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the repulsive guidance molecule (RGM) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="H2Y1F2"/>
    <dbReference type="STRING" id="7719.ENSCINP00000035736"/>
    <dbReference type="Ensembl" id="ENSCINT00000034636.1">
        <property type="protein sequence ID" value="ENSCINP00000035736.1"/>
        <property type="gene ID" value="ENSCING00000021238.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183112"/>
    <dbReference type="HOGENOM" id="CLU_032775_1_0_1"/>
    <dbReference type="InParanoid" id="H2Y1F2"/>
    <dbReference type="OMA" id="DLHYHST"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0015026">
        <property type="term" value="F:coreceptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030509">
        <property type="term" value="P:BMP signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1000.10">
        <property type="entry name" value="Mog1/PsbP, alpha/beta/alpha sandwich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040287">
        <property type="entry name" value="RGM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009496">
        <property type="entry name" value="RGM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010536">
        <property type="entry name" value="RGM_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31428:SF6">
        <property type="entry name" value="REPULSIVE GUIDANCE MOLECULE B HOMOLOG DRAG-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31428">
        <property type="entry name" value="RGM DOMAIN FAMILY MEMBER DRAG-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06534">
        <property type="entry name" value="RGM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06535">
        <property type="entry name" value="RGM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Repulsive guidance molecule N-terminal" evidence="11">
        <location>
            <begin position="2"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="domain" description="Repulsive guidance molecule C-terminal" evidence="10">
        <location>
            <begin position="93"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="46"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="249"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="51"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF06534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF06535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000035736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="345" mass="38759" checksum="766AD9C816994637" modified="2012-04-18" version="1">AEQTRQRCRALRKYRRCIDKNHLPCRGNLFFHSVEHINSRQLEEYSCTNSKTKNPPRTTATPRTKPPPQNTTTMPPKRHFPEDEAAVEGKSAFRHCGMFGDPHLRTFHDEFFTCNVVKAWPLIDNDYLVVMATNIPVTNGSAVTATTKITVLIKASSPCTEQKFYLANNSKLSPSFTDGTISSGSVSVDEVIPGRHVEIQVRFIGTTIIIRKVGSFLTFAIRMPHEFIEGLDPSRQLCVQGCPSRHRIDLKASNKGHGNTLLPGTKGSRRNAAIPPVPKAEQKKIFTEEEARQKCQQIMLAETSPIDEFYFDSCVFDLLTTGDVNFTLSARAALEDVRRTHTLRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>H2Y1W0</accession>
    <name>H2Y1W0_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Myotubularin phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class myotubularin subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="H2Y1W0"/>
    <dbReference type="STRING" id="7719.ENSCINP00000035895"/>
    <dbReference type="Ensembl" id="ENSCINT00000036049.1">
        <property type="protein sequence ID" value="ENSCINP00000035895.1"/>
        <property type="gene ID" value="ENSCING00000024080.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000169809"/>
    <dbReference type="HOGENOM" id="CLU_052409_0_0_1"/>
    <dbReference type="InParanoid" id="H2Y1W0"/>
    <dbReference type="OMA" id="HAYPANV"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030564">
        <property type="entry name" value="Myotubularin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010569">
        <property type="entry name" value="Myotubularin-like_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10807:SF110">
        <property type="entry name" value="FI17948P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10807">
        <property type="entry name" value="MYOTUBULARIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06602">
        <property type="entry name" value="Myotub-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51339">
        <property type="entry name" value="PPASE_MYOTUBULARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myotubularin phosphatase" evidence="2">
        <location>
            <begin position="197"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000035895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="374" mass="43106" checksum="167FF1BE54664F4D" modified="2012-04-18" version="1">MAESGFKSYVDIAEKEHKRNEKLTEAYKKVYKQPQQELEGHLLPGEIAIAHSKKVIKYSAHDDPSKGISGTLVCTNFRVTFITTEKPKYDLVEINNSLLAPQDILLNNVEHVYVEYRNKRKKLTPGSKVKDGIERLELHCKDFRIVAFNFKLTIKGEEKTIIKAILHHAYPANVTLLFAFDFQHNQDVNAIISTPLYRNHRDWMESLKRCNNDQSWRVSSINENFTVSPTMGAYMVVPKSLTDNDLSNIVHQYMNDRIPQWCWSFVNGCSIVRSSELRSESEVGGQMEVKFHAAIQSTGSESRNIRVIDLSKQCPNPIQIQQSFIKLRNLCMPASESEYWESDGHWFSLVHNSKWLHHVNTMLSVANDVCMWMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>H2Y2H3</accession>
    <name>H2Y2H3_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="H2Y2H3"/>
    <dbReference type="STRING" id="7719.ENSCINP00000036108"/>
    <dbReference type="MEROPS" id="S01.079"/>
    <dbReference type="Ensembl" id="ENSCINT00000036843.1">
        <property type="protein sequence ID" value="ENSCINP00000036108.1"/>
        <property type="gene ID" value="ENSCING00000024704.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244883"/>
    <dbReference type="HOGENOM" id="CLU_1969751_0_0_1"/>
    <dbReference type="InParanoid" id="H2Y2H3"/>
    <dbReference type="OMA" id="HICETIS"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000054">
        <property type="entry name" value="Complement C1r subcomponent"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050127">
        <property type="entry name" value="Serine_Proteases_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24264:SF54">
        <property type="entry name" value="PEPTIDASE S1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24264">
        <property type="entry name" value="TRYPSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0720">Serine protease</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Peptidase S1" evidence="9">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000036108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="127" mass="13848" checksum="150B4BE7D5007102" modified="2012-04-18" version="1">MKFVSDNECTGVGESQFCATYAAGGVDACQGDSGGPLVCSNNSIFHLHGVTSWGVGCAGVEQPGVYTRVTGYLGWITHVMNTYQELHMENPSTFKKHGNYFHICETISCRNVFKQVYGLREAGNITR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>H2Y2I3</accession>
    <name>H2Y2I3_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Granzyme M</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Met-ase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Natural killer cell granular protease</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">wild type</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Cleaves peptide substrates after methionine, leucine, and norleucine. Physiological substrates include EZR, alpha-tubulins and the apoptosis inhibitor BIRC5/Survivin. Promotes caspase activation and subsequent apoptosis of target cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="EAAA01002412">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2Y2I3"/>
    <dbReference type="STRING" id="7719.ENSCINP00000036118"/>
    <dbReference type="Ensembl" id="ENSCINT00000033020.1">
        <property type="protein sequence ID" value="ENSCINP00000036118.1"/>
        <property type="gene ID" value="ENSCING00000020515.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153216"/>
    <dbReference type="HOGENOM" id="CLU_006842_7_0_1"/>
    <dbReference type="InParanoid" id="H2Y2I3"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000146">
        <property type="entry name" value="Serine protease 53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252">
        <property type="entry name" value="ACROSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252:SF7">
        <property type="entry name" value="HYALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0720">Serine protease</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Peptidase S1" evidence="13">
        <location>
            <begin position="15"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00054080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00067130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00078807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00079711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000036118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="256" mass="28758" checksum="CF49B277C81230F2" modified="2012-04-18" version="1">CGVQANQPAFPTGRIVGGSEAVPHSWPWAVSINLLRSKYNFWKCGGTIIHNDWIVTAAHCFANKPTTNYVVAVGRHDQNDLTSLPGDVYEIKRIVYHPDYDDPSNKFDVALLELKRSITYRREVAPACLPNPDVIPSTGGQCWLVGWGFTSNEATSPRNILKQAVQVIESPSTCQQVYKRYYYDPNTNLCSKRSESENVNMQCRGDSGGPLVCFENERWVLYGVSNFGPKTNCVYFNLYSRVGALNSFSIKRFVNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>H2Y2U4</accession>
    <name>H2Y2U4_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">MICOS complex subunit MIC13</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">wild type</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the mitochondrial contact site and cristae organizing system (MICOS) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Mitochondrion inner membrane</location>
            <topology evidence="1 9">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the MICOS complex subunit Mic13 family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001098">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2Y2U4"/>
    <dbReference type="STRING" id="7719.ENSCINP00000036229"/>
    <dbReference type="Ensembl" id="ENSCINT00000032021.1">
        <property type="protein sequence ID" value="ENSCINP00000036229.1"/>
        <property type="gene ID" value="ENSCING00000021457.1"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2290665_0_0_1"/>
    <dbReference type="InParanoid" id="H2Y2U4"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061617">
        <property type="term" value="C:MICOS complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044284">
        <property type="term" value="C:mitochondrial crista junction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042407">
        <property type="term" value="P:cristae formation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026769">
        <property type="entry name" value="Mic13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31816">
        <property type="entry name" value="MICOS COMPLEX SUBUNIT MIC13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31816:SF3">
        <property type="entry name" value="MICOS COMPLEX SUBUNIT MIC13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15884">
        <property type="entry name" value="QIL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000036229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="101" mass="10768" checksum="05574D72B61D09CE" modified="2012-04-18" version="1">MALWSATKFVAKGGVLGGAVYLTTKNGIWGENPDTGIRAIKQFSKSVDGTLGGEYMGKVTQLPQLNATEKWNKAVSYSIDCVADSPAIAAKAYKTIKDKII</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>H2Y2Z0</accession>
    <name>H2Y2Z0_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="H2Y2Z0"/>
    <dbReference type="Ensembl" id="ENSCINT00000031801.1">
        <property type="protein sequence ID" value="ENSCINP00000036275.1"/>
        <property type="gene ID" value="ENSCING00000019489.1"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1277235_0_0_1"/>
    <dbReference type="InParanoid" id="H2Y2Z0"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="81"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000036275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="216" mass="25244" checksum="33F527FD4C735C31" modified="2012-04-18" version="1">MQKHSDDMTAKMEKWASGASNYEKQVIYKMLAQVRPNNTHSPSTPCTFNQDGADQFVEANVPSPEYDQPYEQYPSLTDYSKRKKRNVHSAPIKKMGGLVTSSTQHPPRDQRIYRLKGQDTRVDPSPEEERHLNGYMNQLQQSQFHQSKTDRKYARVPMHTYMPRHSTGAQDYQCKVSFFQSVRSPFKNHFVIHPDFTSETVKKRKPCSHTITHFRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>H2Y3U6</accession>
    <name>H2Y3U6_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Dynein heavy chain C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01002906">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2Y3U6"/>
    <dbReference type="STRING" id="7719.ENSCINP00000036582"/>
    <dbReference type="Ensembl" id="ENSCINT00000034265.1">
        <property type="protein sequence ID" value="ENSCINP00000036582.1"/>
        <property type="gene ID" value="ENSCING00000023883.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155282"/>
    <dbReference type="HOGENOM" id="CLU_000038_1_3_1"/>
    <dbReference type="InParanoid" id="H2Y3U6"/>
    <dbReference type="OMA" id="MGTPPVF"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.280:FF:000038">
        <property type="entry name" value="AT13908p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.720:FF:000001">
        <property type="entry name" value="dynein heavy chain 7, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.490.20:FF:000001">
        <property type="entry name" value="dynein heavy chain 7, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.280:FF:000037">
        <property type="entry name" value="Dynein, axonemal, heavy chain 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.720">
        <property type="entry name" value="Region D6 of dynein motor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041658">
        <property type="entry name" value="AAA_lid_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042219">
        <property type="entry name" value="AAA_lid_11_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026983">
        <property type="entry name" value="DHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041228">
        <property type="entry name" value="Dynein_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043160">
        <property type="entry name" value="Dynein_C_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22878:SF66">
        <property type="entry name" value="DYNEIN AXONEMAL HEAVY CHAIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22878">
        <property type="entry name" value="DYNEIN HEAVY CHAIN 6, AXONEMAL-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18198">
        <property type="entry name" value="AAA_lid_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18199">
        <property type="entry name" value="Dynein_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynein heavy chain AAA lid" evidence="1">
        <location>
            <begin position="37"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain C-terminal" evidence="2">
        <location>
            <begin position="182"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000036582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="486" mass="55442" checksum="1F479807BF71E7F2" modified="2012-04-18" version="1">MTNEPPKGLKSNILRSYLSDPISDPEFFTSCKQPVAFKRLLFGLCFFHALVQERRQFGPLGWNIPYEFNETDLRISVRQLHMFLNQYEFVPISALKYLAGECNYGGRVTDDWDRRTLNSVLQRGYSDNVVNADTWSFDENELYHRPPEGDWESYLEFCKNLPLLAEPQVFGMHANADITKDQNATQELFNSILLTLARASGGGGDSNDTLVYNVSGDILEKLPPNFDTEAALRRYPTEYKQSMNTVLVQEMVRFNVLTTTIRNSLVNVQKAIKGLVVMSGDLEEVLNSILQGKIPQMWMKKSYPSLKPLGGYTNDFLERLNFLQTWYENGPPPQFWVSGFFFTQAFLTGVQQNFARKYTIPIDLLGFDYVVLEDKEYKTPPDDGAYIYGLYVEGARWDRKAKRLAESIPKALHDPMPKILLVPSKKSDIPERPSYLAPVYKTTERRGVLSTTGHSTNFVIGMSLNSDKPPEHWIGRGVALICQLDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>F6PIV0</accession>
    <name>F6PIV0_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">dolichyl-phosphate-mannose--protein mannosyltransferase</fullName>
            <ecNumber evidence="5">2.4.1.109</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">wild type</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TMTC family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.109" evidence="5"/>
    <dbReference type="EMBL" id="EAAA01001353">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="F6PIV0"/>
    <dbReference type="STRING" id="7719.ENSCINP00000020208"/>
    <dbReference type="Ensembl" id="ENSCINT00000020208.3">
        <property type="protein sequence ID" value="ENSCINP00000020208.3"/>
        <property type="gene ID" value="ENSCING00000010088.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157538"/>
    <dbReference type="HOGENOM" id="CLU_011615_6_0_1"/>
    <dbReference type="InParanoid" id="F6PIV0"/>
    <dbReference type="OMA" id="CRITHRC"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 2"/>
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        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:dolichyl-phosphate-mannose-protein mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
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        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="TMTC_DUF1736"/>
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        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44395">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44395:SF1">
        <property type="entry name" value="PROTEIN O-MANNOSYL-TRANSFERASE TMTC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08409">
        <property type="entry name" value="TMTC_DUF1736"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="12 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 13" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="6"/>
            <end position="23"/>
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    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="95"/>
            <end position="112"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="118"/>
            <end position="137"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="149"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1736" evidence="15">
        <location>
            <begin position="30"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="13">
        <location>
            <begin position="217"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007882"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022803"/>
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    </evidence>
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        <source>
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    </evidence>
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            <dbReference type="Pfam" id="PF08409"/>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000020208.3"/>
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    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    </evidence>
    <sequence length="290" mass="33208" checksum="3B0E60B291B52E4C" modified="2012-04-18" version="2">MKLKPLFLRITACVGMGICLIYARMRLMQGALPHFTGFDNPASSEKFPARHLTYNHLLSLNSLLLLNPYNLLCDWSMGTVPIITSFFDVRNVGALYFWVLYIIIGLHAVFSHTFVSRVASFCISVITLPFLPASNLFIPVGFVVAERILYIPSIGFCLLVALGFFKLQLKLKKKFLVWFLFSCLIAAHSMKTIHRNFEWISERHLFKSGLKITQRNAKIWNNVGHTYEDENDWKTALWFFKQATLVQPDDIGAHMNSGRMFENMNDFVAAEKCYKEAKSHLPKPVPGQSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>F6PRI9</accession>
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            <fullName evidence="6">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
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            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">wild type</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
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    <dbReference type="EMBL" id="EAAA01002669">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="F6PRI9"/>
    <dbReference type="Ensembl" id="ENSCINT00000013081.3">
        <property type="protein sequence ID" value="ENSCINP00000013081.3"/>
        <property type="gene ID" value="ENSCING00000006347.3"/>
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    <dbReference type="HOGENOM" id="CLU_023132_3_2_1"/>
    <dbReference type="InParanoid" id="F6PRI9"/>
    <dbReference type="OMA" id="STICWTG"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 8"/>
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    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17399">
        <property type="entry name" value="MFS_MFSD7"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049680">
        <property type="entry name" value="FLVCR1-2_SLC49-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10924">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10924:SF6">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 49 MEMBER A3"/>
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    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="162"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="71"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="85"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000025008.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="223" mass="24912" checksum="AC0DDE77A30B4E15" modified="2011-07-27" version="1">MSEDEQKSYKRKLSENDDRPNLLSGSEKKKKKRENSSNSSRPKYYPLSERQQLALLMQMTAEEKPASDVDSTPTSSSRRTPGSSGRKDKVNKRNERGETPLHLATIRGDMHAIVDLIKQGADINVQDYAGWTPLHEACNHGYSEIAKFLLESGANVNSKGLDDDYPLHDAVINAHSDLVHLLLKHGANPLLPNKHGERPLDLGDDESIRKMMQAEVILSDESD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>F6QJ41</accession>
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        <recommendedName>
            <fullName evidence="7">alpha-1,2-Mannosidase</fullName>
            <ecNumber evidence="7">3.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100179038</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
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            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Ca(2+)</name>
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        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
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    <comment type="similarity">
        <text evidence="2 7">Belongs to the glycosyl hydrolase 47 family.</text>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:ubiquitin-dependent glycoprotein ERAD pathway"/>
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        <property type="entry name" value="6hp_glycosidase-like_sf"/>
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        <property type="entry name" value="Glyco_hydro_47"/>
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        <property type="entry name" value="PA_dom_sf"/>
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        <property type="entry name" value="PA_domain"/>
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        <property type="entry name" value="Seven-hairpin_glycosidases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45679">
        <property type="entry name" value="ER DEGRADATION-ENHANCING ALPHA-MANNOSIDASE-LIKE PROTEIN 2"/>
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    </dbReference>
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        <property type="entry name" value="ER DEGRADATION-ENHANCING ALPHA-MANNOSIDASE-LIKE PROTEIN 3"/>
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    </dbReference>
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        <property type="entry name" value="Glyco_hydro_47"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Seven-hairpin glycosidases"/>
        <property type="match status" value="1"/>
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    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
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        <location>
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        <location>
            <position position="457"/>
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        <ligand>
            <name>Ca(2+)</name>
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        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR601382-2"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU361193"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF02225"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000018401.3"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="752" mass="83793" checksum="81EAE68A07AC6020" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>F6QJA2</accession>
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            <fullName evidence="7">Peroxisomal sarcosine oxidase-like</fullName>
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        <name evidence="7" type="primary">LOC100183557</name>
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        <name type="scientific">Ciona intestinalis</name>
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    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MSOX/MTOX family.</text>
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        <property type="protein sequence ID" value="ENSCINP00000020711.3"/>
        <property type="gene ID" value="ENSCING00000021687.1"/>
    </dbReference>
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    <dbReference type="InParanoid" id="F6QJA2"/>
    <dbReference type="OMA" id="WPMLWAH"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050031">
        <property type="term" value="F:L-pipecolate oxidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008115">
        <property type="term" value="F:sarcosine oxidase activity"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0033514">
        <property type="term" value="P:L-lysine catabolic process to acetyl-CoA via L-pipecolate"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045170">
        <property type="entry name" value="MTOX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10961">
        <property type="entry name" value="PEROXISOMAL SARCOSINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10961:SF46">
        <property type="entry name" value="PEROXISOMAL SARCOSINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="6">
        <location>
            <begin position="14"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000020711.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="312" mass="35845" checksum="49EBCD7261039503" modified="2012-04-18" version="2">SFFTFTQYQRSHTRGSSHGQSRIIRKLYVDQHHAQLMDEAYQLWNELQTSSGIDFFKINGHIAVCKKSVGKIKKLVASCEKYGIEHKVLDGSHVNAEFPGLKFNDDFEAVYEQTGGVLNADKCLQAIQEELIKFGGELHENEKVLNIEPINASRVNVQTSTAEYDSASVVLCCGSWTNKLLKPLDLQLPLKACKVQVTYWKEKVPNIYSKFPSLIYYKVDAVSMYGLPCEAYPGYFKFCFHYHFVVDPDDEEDLEKHPSSEEAKMRMEEIKEVVKNHLPYLVPEPKVKEPCMLTTTPDTDYILDRHPRHKNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>F6QY65</accession>
    <name>F6QY65_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">snRNA-activating protein complex subunit 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="F6QY65"/>
    <dbReference type="STRING" id="7719.ENSCINP00000020376"/>
    <dbReference type="Ensembl" id="ENSCINT00000020376.3">
        <property type="protein sequence ID" value="ENSCINP00000020376.3"/>
        <property type="gene ID" value="ENSCING00000014913.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000018691"/>
    <dbReference type="HOGENOM" id="CLU_067254_2_0_1"/>
    <dbReference type="InParanoid" id="F6QY65"/>
    <dbReference type="OMA" id="LWREANF"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019185">
        <property type="term" value="C:snRNA-activating protein complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042795">
        <property type="term" value="P:snRNA transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042796">
        <property type="term" value="P:snRNA transcription by RNA polymerase III"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019188">
        <property type="entry name" value="SNAPC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15131">
        <property type="entry name" value="SMALL NUCLEAR RNA ACTIVATING COMPLEX, POLYPEPTIDE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15131:SF3">
        <property type="entry name" value="SNRNA-ACTIVATING PROTEIN COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09808">
        <property type="entry name" value="SNAPC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000020376.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="216" mass="25534" checksum="64F961B1FAFF103C" modified="2012-04-18" version="2">MKLQKKRVPRRFLYPATGMLSDVETLLQKFVDTKSIRFEEFSRLWREANFTLIHAGREGMREKREFLDESFKIILKFVLPPYSLQVRVGAVYTMYSLYLTQKHEPKLKVRMPLSCWKDFVELHQEVTSHKHLDADYVMMTMVKKYQMFEFVAYPSPLSMEDLYNQDSEEDLLTESTSNSAAEFTLLSRLLDEDSIGQLTAVHDKYHQLKCQLNALR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>F6QZI8</accession>
    <name>F6QZI8_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Sterol regulatory element-binding protein cleavage-activating protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">LOC100181007</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="20">Escort protein required for cholesterol as well as lipid homeostasis. Regulates export of the SCAP-SREBP complex from the endoplasmic reticulum to the Golgi upon low cholesterol, thereby regulating the processing of sterol regulatory element-binding proteins (SREBPs) SREBF1/SREBP1 and SREBF2/SREBP2. At high sterol concentrations, formation of a ternary complex with INSIG (INSIG1 or INSIG2) leads to mask the ER export signal in SCAP, promoting retention of the complex in the endoplasmic reticulum. Low sterol concentrations trigger release of INSIG, a conformational change in the SSD domain of SCAP, unmasking of the ER export signal, promoting recruitment into COPII-coated vesicles and transport of the SCAP-SREBP to the Golgi: in the Golgi, SREBPs are then processed, releasing the transcription factor fragment of SREBPs from the membrane, its import into the nucleus and up-regulation of LDLR, INSIG1 and the mevalonate pathway. Binds cholesterol via its SSD domain.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">COPII-coated vesicle membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Golgi apparatus membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the WD repeat SCAP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="F6QZI8"/>
    <dbReference type="FunCoup" id="F6QZI8">
        <property type="interactions" value="84"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000016846"/>
    <dbReference type="Ensembl" id="ENSCINT00000016846.3">
        <property type="protein sequence ID" value="ENSCINP00000016846.3"/>
        <property type="gene ID" value="ENSCING00000008250.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158130"/>
    <dbReference type="HOGENOM" id="CLU_006510_0_0_1"/>
    <dbReference type="InParanoid" id="F6QZI8"/>
    <dbReference type="OMA" id="QECCKIA"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012507">
        <property type="term" value="C:ER to Golgi transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032936">
        <property type="term" value="C:SREBP-SCAP complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032934">
        <property type="term" value="F:sterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008203">
        <property type="term" value="P:cholesterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045540">
        <property type="term" value="P:regulation of cholesterol biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032933">
        <property type="term" value="P:SREBP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057042">
        <property type="entry name" value="Beta-prop_SCAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053958">
        <property type="entry name" value="HMGCR/SNAP/NPC1-like_SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030225">
        <property type="entry name" value="SCAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000731">
        <property type="entry name" value="SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46378">
        <property type="entry name" value="STEROL REGULATORY ELEMENT-BINDING PROTEIN CLEAVAGE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46378:SF1">
        <property type="entry name" value="STEROL REGULATORY ELEMENT-BINDING PROTEIN CLEAVAGE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Beta-prop_SCAP"/>
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        <property type="entry name" value="Sterol-sensing"/>
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        <property type="entry name" value="WD40"/>
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        <property type="entry name" value="WD_REPEATS_2"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0153">Cholesterol metabolism</keyword>
    <keyword evidence="19" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="13" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="15 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="18" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="16" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="8 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 22" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7 21" id="KW-0853">WD repeat</keyword>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="46"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="76"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="111"/>
            <end position="132"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="153"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="178"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="277"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="442"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="600"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="domain" description="SSD" evidence="23">
        <location>
            <begin position="45"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="21">
        <location>
            <begin position="780"/>
            <end position="820"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007410"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019541"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022548"/>
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        <source>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00045958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000016846.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="945" mass="106962" checksum="3893085C410FDF48" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>F6R1K9</accession>
    <accession>A0A1W2W4H7</accession>
    <name>F6R1K9_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">3-oxo-5-alpha-steroid 4-dehydrogenase</fullName>
            <ecNumber evidence="18">1.3.1.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">LOC100176622</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">wild type</strain>
        </source>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Converts testosterone (T) into 5-alpha-dihydrotestosterone (DHT) and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology.</text>
    </comment>
    <comment type="function">
        <text evidence="18">Converts testosterone into 5-alpha-dihydrotestosterone and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>17beta-hydroxy-5alpha-androstan-3-one + NADP(+) = testosterone + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50820"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16330"/>
            <dbReference type="ChEBI" id="CHEBI:17347"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.3.1.22"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="17">
            <dbReference type="Rhea" id="RHEA:50822"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>5alpha-pregnane-3,20-dione + NADP(+) = progesterone + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21952"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17026"/>
            <dbReference type="ChEBI" id="CHEBI:28952"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.3.1.22"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="16">
            <dbReference type="Rhea" id="RHEA:21954"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15 18">
            <text>a 3-oxo-5alpha-steroid + NADP(+) = a 3-oxo-Delta(4)-steroid + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54384"/>
            <dbReference type="ChEBI" id="CHEBI:13601"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:47909"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.3.1.22"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Microsome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 18">Belongs to the steroid 5-alpha reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.22" evidence="18"/>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002121013.1">
        <property type="nucleotide sequence ID" value="XM_002120977.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6R1K9"/>
    <dbReference type="Ensembl" id="ENSCINT00000001520.3">
        <property type="protein sequence ID" value="ENSCINP00000001520.3"/>
        <property type="gene ID" value="ENSCING00000000832.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100176622"/>
    <dbReference type="KEGG" id="cin:100176622"/>
    <dbReference type="GeneTree" id="ENSGT00950000182886"/>
    <dbReference type="HOGENOM" id="CLU_065395_1_0_1"/>
    <dbReference type="InParanoid" id="F6R1K9"/>
    <dbReference type="OMA" id="IASNFHF"/>
    <dbReference type="OrthoDB" id="5788137at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047751">
        <property type="term" value="F:3-oxo-5-alpha-steroid 4-dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
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    <dbReference type="GO" id="GO:0003865">
        <property type="term" value="F:3-oxo-5-alpha-steroid 4-dehydrogenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0006702">
        <property type="term" value="P:androgen biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0008584">
        <property type="term" value="P:male gonad development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="entry name" value="Steroid 5-alpha reductase, putative"/>
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        <property type="entry name" value="3-oxo-5-alpha-steroid_4-DH"/>
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        <property type="entry name" value="3-oxo-5_a-steroid_4-DH_C"/>
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    <dbReference type="InterPro" id="IPR039357">
        <property type="entry name" value="SRD5A/TECR"/>
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    <dbReference type="PANTHER" id="PTHR10556">
        <property type="entry name" value="3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10556:SF37">
        <property type="entry name" value="3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE 2"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF02544">
        <property type="entry name" value="Steroid_dh"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="5_alpha-SR2"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS50244">
        <property type="entry name" value="S5A_REDUCTASE"/>
        <property type="match status" value="1"/>
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    <keyword evidence="5" id="KW-0221">Differentiation</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0492">Microsome</keyword>
    <keyword evidence="8" id="KW-0521">NADP</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0726">Sexual differentiation</keyword>
    <keyword evidence="4 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="52"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="79"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="110"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="142"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="3-oxo-5-alpha-steroid 4-dehydrogenase C-terminal" evidence="19">
        <location>
            <begin position="109"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00007742"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022928"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00045549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048164"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00048292"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00049397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRNR" id="PIRNR015596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF02544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000001520.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="258" mass="29515" checksum="417C832F85F75362" modified="2012-04-18" version="2">MFELETDLRIIKWMSGSIFMAGILSLAAYQVGVPSGYGKHTKKSKFTVDARLAWVIQECPSFFLPVLVTYSVGVENINFSNLLIISAFAFHYFQRSFIYPMLMNRMSTPLPLRIVFMSFTFTFINGYAISKYEAAHVSLPENWINSFQFLTGMLMWASGLLINIHSDHVLRNLRKPNEVGYKIPRGGAFEYVSGANFSGEIFEWFGLLIASNFHFPLICFSLSTSLTIGTRALFHHKFYLSKFEDYPKNRKAVIPFVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>F6R4H7</accession>
    <name>F6R4H7_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">Origin recognition complex subunit 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the origin recognition complex (ORC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the ORC2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="F6R4H7"/>
    <dbReference type="STRING" id="7719.ENSCINP00000004858"/>
    <dbReference type="Ensembl" id="ENSCINT00000004858.2">
        <property type="protein sequence ID" value="ENSCINP00000004858.2"/>
        <property type="gene ID" value="ENSCING00000002383.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015228"/>
    <dbReference type="HOGENOM" id="CLU_018596_1_0_1"/>
    <dbReference type="InParanoid" id="F6R4H7"/>
    <dbReference type="OMA" id="YDFELAY"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005664">
        <property type="term" value="C:nuclear origin of replication recognition complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007220">
        <property type="entry name" value="ORC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056772">
        <property type="entry name" value="RecA-like_ORC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056773">
        <property type="entry name" value="WHD_ORC2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14052">
        <property type="entry name" value="ORIGIN RECOGNITION COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ORIGIN RECOGNITION COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04084">
        <property type="entry name" value="RecA-like_ORC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24882">
        <property type="entry name" value="WHD_ORC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Origin recognition complex subunit 2 RecA-like" evidence="7">
        <location>
            <begin position="2"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="Origin recognition complex subunit 2 winged-helix" evidence="8">
        <location>
            <begin position="245"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU368084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF24882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000004858.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="315" mass="35690" checksum="D82EFFD47B430C06" modified="2012-04-18" version="2">AKLFDGYKSCFREWMFYLCSNFNLLLFGLGSKRQIIHSFCDSMLKNQNKLILNGFFPGLTIKSSILVWAKIVCNIMKCTVIHGNIKKYMETTAAHSQDEMLNTITKHYNSDDEKSDHLFLIIHNIDGAPLRTSNSQGALCKLADIKRIHIIASIDHINAPLAWDQSKLTRFRWLWHDVTTYASYTAETSYEGSLLLSSAGAGLGARGAALALSGLLHVAKSLTSNARGVFKILIEHQLEEKDEEETQEAFSFTDLYRICRERFLVNSELTLKAHLTEFVDHKLVNIHKGSDGGEYLSVPLDPTTLKEYLEYDFNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>F6R4U4</accession>
    <name>F6R4U4_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Claudin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">wild type</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell junction</location>
            <location evidence="8">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the claudin family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01000514">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6R4U4"/>
    <dbReference type="STRING" id="7719.ENSCINP00000016825"/>
    <dbReference type="Ensembl" id="ENSCINT00000016825.3">
        <property type="protein sequence ID" value="ENSCINP00000016825.3"/>
        <property type="gene ID" value="ENSCING00000008241.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160672"/>
    <dbReference type="HOGENOM" id="CLU_076370_1_2_1"/>
    <dbReference type="InParanoid" id="F6R4U4"/>
    <dbReference type="OMA" id="AMSYDKK"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
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        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0070830">
        <property type="term" value="P:bicellular tight junction assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006187">
        <property type="entry name" value="Claudin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017974">
        <property type="entry name" value="Claudin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004031">
        <property type="entry name" value="PMP22/EMP/MP20/Claudin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12002">
        <property type="entry name" value="CLAUDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00822">
        <property type="entry name" value="PMP22_Claudin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01077">
        <property type="entry name" value="CLAUDIN"/>
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        <property type="entry name" value="CLAUDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="2 8" id="KW-0796">Tight junction</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="9"/>
            <end position="27"/>
        </location>
    </feature>
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        <location>
            <begin position="83"/>
            <end position="105"/>
        </location>
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        <location>
            <begin position="117"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="164"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU060637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000016825.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="187" mass="20048" checksum="A6BA61FB4CE74D22" modified="2012-04-18" version="2">MAISSCQIVGYLVGILTWLGALLVVTLPDWKHSYVSERSFSGVLVVRGLWTTCTVKESGSVECINISEPVFALPTLIKGCRTLSVLGTVLAFCAVAITPLGMRCTRIGGDAKTKLRIARCCAVLFGLAAILIGIAVSWYAEDVAKDFHNPLSLRKLPPVSDLKYVYGNALYIGWATMATGAISSTFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>F6RPK9</accession>
    <accession>A0A1W2WNV1</accession>
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        <submittedName>
            <fullName evidence="2">Uncharacterized LOC100184570</fullName>
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    </protein>
    <gene>
        <name evidence="2" type="primary">LOC100184570</name>
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    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01000565">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002131084.1">
        <property type="nucleotide sequence ID" value="XM_002131048.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6RPK9"/>
    <dbReference type="Ensembl" id="ENSCINT00000025662.2">
        <property type="protein sequence ID" value="ENSCINP00000025416.2"/>
        <property type="gene ID" value="ENSCING00000013960.2"/>
    </dbReference>
    <dbReference type="GeneID" id="100184570"/>
    <dbReference type="KEGG" id="cin:100184570"/>
    <dbReference type="GeneTree" id="ENSGT00390000002585"/>
    <dbReference type="HOGENOM" id="CLU_1916337_0_0_1"/>
    <dbReference type="InParanoid" id="F6RPK9"/>
    <dbReference type="OMA" id="ELTSYIW"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="14"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000025416.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="138" mass="15843" checksum="3C8ECDBDFBE8F707" modified="2011-07-27" version="1">MTGMQRSSTTLNKLWLPVGIVVFVGLTFGLLYGMDLISGRGSRKFTYKQYLEQSLTCADQFSIRLLKQTNFFVDQMMPMQKFCREWEIYVTCRQRVASYTELTSYIWNNGTLVQAMCSGNITDQEPMDAGMLNFISVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>F6RVH7</accession>
    <accession>A0A1W2WDK1</accession>
    <name>F6RVH7_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized LOC100179010</fullName>
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        <name evidence="1" type="primary">LOC100179010</name>
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    <organism evidence="1 2">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
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            <taxon>Cionidae</taxon>
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        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_002125599.1">
        <property type="nucleotide sequence ID" value="XM_002125563.3"/>
    </dbReference>
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    <dbReference type="STRING" id="7719.ENSCINP00000027777"/>
    <dbReference type="Ensembl" id="ENSCINT00000028023.2">
        <property type="protein sequence ID" value="ENSCINP00000027777.2"/>
        <property type="gene ID" value="ENSCING00000015824.2"/>
    </dbReference>
    <dbReference type="GeneID" id="100179010"/>
    <dbReference type="KEGG" id="cin:100179010"/>
    <dbReference type="GeneTree" id="ENSGT00940000166373"/>
    <dbReference type="HOGENOM" id="CLU_061199_2_2_1"/>
    <dbReference type="InParanoid" id="F6RVH7"/>
    <dbReference type="OMA" id="RDLDAWH"/>
    <dbReference type="OrthoDB" id="272681at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040632">
        <property type="entry name" value="Sulfotransfer_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36978:SF4">
        <property type="entry name" value="P-LOOP CONTAINING NUCLEOSIDE TRIPHOSPHATE HYDROLASE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36978">
        <property type="entry name" value="P-LOOP CONTAINING NUCLEOTIDE TRIPHOSPHATE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17784">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000027777.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="271" mass="31574" checksum="CF707021B9D9EBF8" modified="2012-04-18" version="2">MKIICAGLSKTGTKTMQSALQELGYNVYDFMENFTILGDEWCKILTKGGTTEEFRQMYEDVDVVSDLPGAYYWDEIFKAFPDAKVILMVRESEDAWYKSLLTQLEAQQSIVFRLMPYISIAGWRMSTYFALMAPSIWGVKYESNLFRLPEVNEMQMRMTYRRHNAYVMQATPKDQLLVYNVKEGWGPICNFLGVPVPDKPFPRKNVRGKIVEKLMKKDPFFIRVQREIMFSISVITVVLSYGVYQAGKLAWYNDWSSTWSQITDYVSSFYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F6SE45</accession>
    <name>F6SE45_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="F6SE45"/>
    <dbReference type="Ensembl" id="ENSCINT00000012725.3">
        <property type="protein sequence ID" value="ENSCINP00000012725.3"/>
        <property type="gene ID" value="ENSCING00000006162.3"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2189828_0_0_1"/>
    <dbReference type="InParanoid" id="F6SE45"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000012725.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="109" mass="12946" checksum="215794C81F5F608A" modified="2011-07-27" version="1">PNCKYNKKWKVKRSLFAIKHPEFAKNKEEREYRTELRKIILSLRDPNNKDDTILDQLHKESQLVSLPKTGTILRTKKLFTLTVAKFLKLQKDYHLRVKKPGSFIIVKVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>F6SG74</accession>
    <name>F6SG74_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">wild type</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bZIP family. CNC subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002274">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6SG74"/>
    <dbReference type="IntAct" id="F6SG74">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000012430"/>
    <dbReference type="Ensembl" id="ENSCINT00000012430.3">
        <property type="protein sequence ID" value="ENSCINP00000012430.3"/>
        <property type="gene ID" value="ENSCING00000006014.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182892"/>
    <dbReference type="HOGENOM" id="CLU_027958_0_0_1"/>
    <dbReference type="InParanoid" id="F6SG74"/>
    <dbReference type="OMA" id="CKLPCST"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14719">
        <property type="entry name" value="bZIP_BACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.880.10">
        <property type="entry name" value="Transcription factor, Skn-1-like, DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043321">
        <property type="entry name" value="bZIP_BACH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004826">
        <property type="entry name" value="bZIP_Maf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047167">
        <property type="entry name" value="NFE2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008917">
        <property type="entry name" value="TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24411">
        <property type="entry name" value="NUCLEAR FACTOR ERYTHROID 2-RELATED FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24411:SF55">
        <property type="entry name" value="SEGMENTATION PROTEIN CAP'N'COLLAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03131">
        <property type="entry name" value="bZIP_Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47454">
        <property type="entry name" value="A DNA-binding domain in eukaryotic transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="9">
        <location>
            <begin position="273"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="451"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="286"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008157"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000012430.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="508" mass="56401" checksum="EA8CC58DC302C241" modified="2012-04-18" version="2">QKLFQETSDPTAVAQCADGSKACKNSTISRKVVMASKTTDPCSEGTECCVETKNPYTSTCEDDVKNSKGDCPLMAKLSLNSKPTSSDVKPSTATPIPDIKPITSVLPPGAETINLSSLPKAQQQMMLQHFLQQQNYRLPCTKTVPSCDQPSYNSQQIRYPTKVPYDPSSYDRPMCTISYSQSSHVNKCGSNYNSDTDEALSADDDSERSFYMQGKCAGNYSSENCGKLRKQSDVVLPFSINEITKLPRTQLQELLNRNPSLSPQQITAIHEIRRRGKNRIAAQRCRKRKMDCIRSLQCQLEQLREEHLNLMGERRTCQDKSLKLAEMFQKRYEQVFSDFKLCKQSSETSEACPETENSDSAPGDHIVASMADYFKMESDNARELAERFCKLPCSTLPPFSLCSVMSQSCPMMSSPCSMMSQQDVVVVCDGDDSVADMCAKPCSPSGCSTNSYTSELTEPHPEVTTVQEGAEKAPSLQGVTHNPPTSPKKMDVDTPRVYVTKETEFRPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>F6SHN0</accession>
    <accession>A0A1W2WF94</accession>
    <name>F6SHN0_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Beta-hexosaminidase</fullName>
            <ecNumber evidence="12">3.2.1.52</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC100178428</name>
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    <organism evidence="18 19">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">wild type</strain>
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    <reference evidence="18" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <reaction evidence="1">
            <text>Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.</text>
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    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>N-acetyl-beta-D-6-sulfogalactosaminyl-(1-&gt;4)-alpha-L-iduronyl-(1-&gt;3)-N-acetyl-D-6-sulfogalactosamine + H2O = alpha-L-iduronyl-(1-&gt;3)-N-acetyl-D-6-sulfogalactosamine + N-acetyl-D-6-sulfogalactosamine</text>
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            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:152567"/>
            <dbReference type="ChEBI" id="CHEBI:152568"/>
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    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N-acetyl-beta-D-galactosaminyl-(1-&gt;4)-beta-D-3-sulfogalactosyl-(1-&gt;4)-beta-D-glucosyl-(1&lt;-&gt;1')-ceramide + H2O = a beta-D-3-sulfogalactosyl-(1-&gt;4)-beta-D-glucosyl-(1&lt;-&gt;1')-ceramide + N-acetyl-beta-D-galactosamine</text>
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            <dbReference type="ChEBI" id="CHEBI:28497"/>
            <dbReference type="ChEBI" id="CHEBI:90163"/>
            <dbReference type="ChEBI" id="CHEBI:90164"/>
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        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:48277"/>
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    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a ganglioside GM2 (d18:1(4E)) + H2O = a ganglioside GM3 (d18:1(4E)) + N-acetyl-beta-D-galactosamine</text>
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            <dbReference type="ChEBI" id="CHEBI:60065"/>
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        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:47941"/>
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    <comment type="catalytic activity">
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            <text>a ganglioside GM2 + H2O = a ganglioside GM3 + N-acetyl-beta-D-galactosamine</text>
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            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28497"/>
            <dbReference type="ChEBI" id="CHEBI:79210"/>
            <dbReference type="ChEBI" id="CHEBI:79218"/>
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        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:47969"/>
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    <comment type="catalytic activity">
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            <text>beta-D-GalNAc-(1-&gt;4)-alpha-L-IdoA-(1-&gt;3)-beta-D-GalNAc-4-sulfate-(1-&gt;4)-alpha-L-IdoA-(1-&gt;3)-D-GalNAc-4-sulfate + H2O = alpha-L-IdoA-(1-&gt;3)-beta-D-GalNAc-4-sulfate-(1-&gt;4)-alpha-L-IdoA-(1-&gt;3)-D-GalNAc-4-sulfate + N-acetyl-D-galactosamine</text>
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            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28037"/>
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            <dbReference type="ChEBI" id="CHEBI:152566"/>
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        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:64373"/>
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    <comment type="similarity">
        <text evidence="2 12">Belongs to the glycosyl hydrolase 20 family.</text>
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        <property type="entry name" value="beta-N-acetylhexosaminidase-like domain"/>
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    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="3 15" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
            <end position="17"/>
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    <feature type="disulfide bond" evidence="14">
        <location>
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            <end position="532"/>
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        <source>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F6SL96</accession>
    <name>F6SL96_CIOIN</name>
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        <recommendedName>
            <fullName evidence="3">NACHT domain-containing protein</fullName>
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    <organism evidence="4 5">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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            <taxon>Tunicata</taxon>
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            <taxon>Phlebobranchia</taxon>
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    <reference evidence="5" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">wild type</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01002305">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6SL96"/>
    <dbReference type="Ensembl" id="ENSCINT00000027737.2">
        <property type="protein sequence ID" value="ENSCINP00000027491.2"/>
        <property type="gene ID" value="ENSCING00000015554.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00980000202028"/>
    <dbReference type="HOGENOM" id="CLU_1242573_0_0_1"/>
    <dbReference type="InParanoid" id="F6SL96"/>
    <dbReference type="OMA" id="HAFEWIL"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051261">
        <property type="entry name" value="NLR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24106">
        <property type="entry name" value="NACHT, LRR AND CARD DOMAINS-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="NACHT" evidence="3">
        <location>
            <begin position="81"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000027491.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="218" mass="24673" checksum="9AB45624916ED287" modified="2012-04-18" version="2">MKQAIEEVIEAIAVKRKVNAIYSHQNHDTPVVTPRLSKVSFYEGGFLPDGDVYTPSTDRSLIGGQGDLTFEELIQLWKEKRYIALIGDPGCGKTVLSDRLETRCGCVCFHLKFMNLNYDSATLITLKELLLDFAHPELDQHTRDLAFEWVMKNQSKCSIMLDGYDQARWAMKENPPKVSYVTKSCVSDVISNLCCGNFLPMTQVLVTSRPYMMLPLPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>F6SLJ7</accession>
    <name>F6SLJ7_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Casein kinase substrate phosphoprotein PP28 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="F6SLJ7"/>
    <dbReference type="FunCoup" id="F6SLJ7">
        <property type="interactions" value="512"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000027477"/>
    <dbReference type="Ensembl" id="ENSCINT00000027723.1">
        <property type="protein sequence ID" value="ENSCINP00000027477.1"/>
        <property type="gene ID" value="ENSCING00000015540.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000018509"/>
    <dbReference type="HOGENOM" id="CLU_084870_1_0_1"/>
    <dbReference type="InParanoid" id="F6SLJ7"/>
    <dbReference type="OMA" id="HYAKLTM"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019380">
        <property type="entry name" value="Casein_kinase_sb_PP28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039876">
        <property type="entry name" value="HAP28"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22055">
        <property type="entry name" value="28 KDA HEAT- AND ACID-STABLE PHOSPHOPROTEIN PDGF-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10252">
        <property type="entry name" value="PP28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Casein kinase substrate phosphoprotein PP28" evidence="2">
        <location>
            <begin position="55"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="124"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="80"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000027477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="155" mass="17926" checksum="D428545DFB170A77" modified="2011-07-27" version="1">MPRGGKRSQKGRARQFTSVDELESQRKQEQNWRKKQDSSSDEDTSEEEEKSDINNPNRVINKTKKASQLDEIVTTPTTDLSRREKEQIAKQEAQRRYQQLHAQGKTDEARADLARLAIIRKQREEAAQKREAEKKAKEERLHASKEASLNAIKGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>F6SPI7</accession>
    <name>F6SPI7_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Leucine-rich repeat protein SHOC-2</fullName>
        </recommendedName>
        <alternativeName>
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        </alternativeName>
        <alternativeName>
            <fullName evidence="4 5">protein Sur-8 homolog</fullName>
        </alternativeName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="F6SPI7"/>
    <dbReference type="FunCoup" id="F6SPI7">
        <property type="interactions" value="42"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000010077"/>
    <dbReference type="Ensembl" id="ENSCINT00000010077.3">
        <property type="protein sequence ID" value="ENSCINP00000010077.3"/>
        <property type="gene ID" value="ENSCING00000004875.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164558"/>
    <dbReference type="HOGENOM" id="CLU_000288_18_23_1"/>
    <dbReference type="InParanoid" id="F6SPI7"/>
    <dbReference type="OMA" id="HNAIPSI"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000116">
        <property type="entry name" value="Leucine-rich repeat-containing protein 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000193">
        <property type="entry name" value="Leucine-rich repeat-containing protein 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF54">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52075">
        <property type="entry name" value="Outer arm dynein light chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>F6SQ23</accession>
    <accession>A0A1W2W560</accession>
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        <submittedName>
            <fullName evidence="7">Transmembrane protein 45B</fullName>
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        <name evidence="7" type="primary">LOC100185183</name>
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    <organism evidence="7 8">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Tunicata</taxon>
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    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">wild type</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM45 family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001532">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="F6SQ23"/>
    <dbReference type="Ensembl" id="ENSCINT00000018500.3">
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        <property type="gene ID" value="ENSCING00000009116.3"/>
    </dbReference>
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    <dbReference type="KEGG" id="cin:100185183"/>
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        <property type="component" value="Chromosome 2"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="entry name" value="DUF716"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042127">
        <property type="entry name" value="TMEM45"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16007">
        <property type="entry name" value="EPIDIDYMAL MEMBRANE PROTEIN E9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16007:SF15">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 45B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04819">
        <property type="entry name" value="DUF716"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
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            <end position="23"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">wild type</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Introduces a single-strand break via transesterification at a target site in duplex DNA. Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand.</text>
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    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.</text>
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        </reaction>
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    <comment type="similarity">
        <text evidence="2 7">Belongs to the type IA topoisomerase family.</text>
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    <dbReference type="EC" id="5.6.2.1" evidence="3 7"/>
    <dbReference type="EMBL" id="EAAA01001916">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6ST44"/>
    <dbReference type="FunCoup" id="F6ST44">
        <property type="interactions" value="507"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000016196"/>
    <dbReference type="Ensembl" id="ENSCINT00000016196.3">
        <property type="protein sequence ID" value="ENSCINP00000016196.3"/>
        <property type="gene ID" value="ENSCING00000007910.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156516"/>
    <dbReference type="HOGENOM" id="CLU_002929_1_0_1"/>
    <dbReference type="InParanoid" id="F6ST44"/>
    <dbReference type="OMA" id="VIHNVYS"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 4"/>
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    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0003917">
        <property type="term" value="F:DNA topoisomerase type I (single strand cut, ATP-independent) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="evidence" value="ECO:0000318"/>
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        <property type="entry name" value="DNA topoisomerase"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Topo_IA"/>
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        <property type="entry name" value="Topo_IA_2"/>
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    <dbReference type="InterPro" id="IPR023406">
        <property type="entry name" value="Topo_IA_AS"/>
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    <dbReference type="InterPro" id="IPR013497">
        <property type="entry name" value="Topo_IA_cen"/>
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    <dbReference type="InterPro" id="IPR013824">
        <property type="entry name" value="Topo_IA_cen_sub1"/>
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    <dbReference type="InterPro" id="IPR013825">
        <property type="entry name" value="Topo_IA_cen_sub2"/>
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    <dbReference type="InterPro" id="IPR013826">
        <property type="entry name" value="Topo_IA_cen_sub3"/>
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    <dbReference type="InterPro" id="IPR023405">
        <property type="entry name" value="Topo_IA_core_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003602">
        <property type="entry name" value="Topo_IA_DNA-bd_dom"/>
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    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_dom"/>
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    <dbReference type="InterPro" id="IPR034144">
        <property type="entry name" value="TOPRIM_TopoIII"/>
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    <dbReference type="InterPro" id="IPR056452">
        <property type="entry name" value="Zn_ribbon_TOP3B"/>
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    <dbReference type="PANTHER" id="PTHR11390:SF20">
        <property type="entry name" value="DNA TOPOISOMERASE 3-BETA-1"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR11390">
        <property type="entry name" value="PROKARYOTIC DNA TOPOISOMERASE"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF01131">
        <property type="entry name" value="Topoisom_bac"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF01751">
        <property type="entry name" value="Toprim"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF23546">
        <property type="entry name" value="Zn_ribbon_TOP3B"/>
        <property type="match status" value="1"/>
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    <dbReference type="PRINTS" id="PR00417">
        <property type="entry name" value="PRTPISMRASEI"/>
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    <dbReference type="SMART" id="SM00437">
        <property type="entry name" value="TOP1Ac"/>
        <property type="match status" value="1"/>
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    <dbReference type="SMART" id="SM00436">
        <property type="entry name" value="TOP1Bc"/>
        <property type="match status" value="1"/>
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    <dbReference type="SMART" id="SM00493">
        <property type="entry name" value="TOPRIM"/>
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    <dbReference type="SUPFAM" id="SSF56712">
        <property type="entry name" value="Prokaryotic type I DNA topoisomerase"/>
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        <property type="entry name" value="TOPO_IA_1"/>
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        <property type="entry name" value="TOPO_IA_2"/>
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    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
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    <keyword evidence="5 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0799">Topoisomerase</keyword>
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        <location>
            <begin position="1"/>
            <end position="118"/>
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    <feature type="domain" description="Topo IA-type catalytic" evidence="10">
        <location>
            <begin position="136"/>
            <end position="559"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="788"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="788"/>
            <end position="806"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009446"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012891"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362092"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50880"/>
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    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS52039"/>
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    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000016196.3"/>
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    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="820" mass="92374" checksum="807995910A8ECA92" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>F6T7K5</accession>
    <name>F6T7K5_CIOIN</name>
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        <recommendedName>
            <fullName evidence="3">Mammalian ependymin-related protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100184676</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">wild type</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Binds anionic lipids and gangliosides at acidic pH.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ependymin family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01000979">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6T7K5"/>
    <dbReference type="FunCoup" id="F6T7K5">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSCINT00000015708.3">
        <property type="protein sequence ID" value="ENSCINP00000015708.3"/>
        <property type="gene ID" value="ENSCING00000007666.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164413"/>
    <dbReference type="HOGENOM" id="CLU_097673_1_0_1"/>
    <dbReference type="InParanoid" id="F6T7K5"/>
    <dbReference type="OMA" id="NQTCCKK"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001299">
        <property type="entry name" value="Ependymin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10697">
        <property type="entry name" value="MAMMALIAN EPENDYMIN-RELATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10697:SF1">
        <property type="entry name" value="MAMMALIAN EPENDYMIN-RELATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00811">
        <property type="entry name" value="Ependymin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00317">
        <property type="entry name" value="EPENDYMIN"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003342236" description="Mammalian ependymin-related protein 1" evidence="4">
        <location>
            <begin position="19"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000015708.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="196" mass="22043" checksum="353672D837846AF0" modified="2012-04-18" version="2">MKLLVLFSFFACVALAAAQTPCVSPGVFEAKAYAFDHHERNFVIGKISYDENQERVRLVDAEYENSNLGQYDTLYAFKEKTQYRVDMKTKECTKSPLTGDYFPFGIFPKSTYTGSFILGSLSGPGTGVIVQEWYYKNADGGSVWVQLYTEHGCLPIQAIFIGHDSAGEEIDYHYEYMDVTLGVQDPAVFDVPKECL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>F6UG85</accession>
    <name>F6UG85_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Echinoderm microtubule-associated protein-like 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100176758</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
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            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
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                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
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                <person name="Larimer F."/>
                <person name="Detter C."/>
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                <person name="Richardson P."/>
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                <person name="Kohara Y."/>
                <person name="Levine M."/>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
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                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
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                <person name="Kano S."/>
                <person name="Kobayashi K."/>
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                <person name="Makabe K.W."/>
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                <person name="Mochizuki Y."/>
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    <sequence length="371" mass="42927" checksum="2DA32541D41CAAB3" modified="2012-04-18" version="2">MLTVPPPFVYNTTATIPNVCTSILPVAQDKWGSTCRPNYSGWQPVAAVIILVLAGLNLVKEAFQLFSQRLSYFRSTTNIIEMALYILSILVVINVNDFSRATGMREPWQWQCGAAALFFSWLGLLLFIQNFPRFGIFVLMFNDVLWTFASFFAVFVLFILGFALSFYVLLMNQVAFSQWWKSIIKTSVMMIGEFEYDGIFNSVPTMVSYQAVTYTLFVVFMIIMSIIIMNLLVGLAVDDIKGVQDNAELERLAMQVKLALEVEFTLPQIFRRRFIRQSRSFFPNLHRRKHWIVRWFHSEEHLNHRTIHDALNPEKSDLQLIDTKVETMGRKLGDLRSMVHDMQERNKGLESMLKAIATNLNVKWEENNDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>F6UK45</accession>
    <name>F6UK45_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">wild type</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9 10">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001669">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="EAAA01001670">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6UK45"/>
    <dbReference type="FunCoup" id="F6UK45">
        <property type="interactions" value="210"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000003405"/>
    <dbReference type="Ensembl" id="ENSCINT00000003405.3">
        <property type="protein sequence ID" value="ENSCINP00000003405.3"/>
        <property type="gene ID" value="ENSCING00000001680.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154801"/>
    <dbReference type="HOGENOM" id="CLU_001485_11_0_1"/>
    <dbReference type="InParanoid" id="F6UK45"/>
    <dbReference type="OMA" id="HNANCEV"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904115">
        <property type="term" value="C:axon cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032839">
        <property type="term" value="C:dendrite cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005871">
        <property type="term" value="C:kinesin complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008574">
        <property type="term" value="F:plus-end-directed microtubule motor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098957">
        <property type="term" value="P:anterograde axonal transport of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098971">
        <property type="term" value="P:anterograde dendritic transport of neurotransmitter receptor complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030951">
        <property type="term" value="P:establishment or maintenance of microtubule cytoskeleton polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007292">
        <property type="term" value="P:female gamete generation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007097">
        <property type="term" value="P:nuclear migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048489">
        <property type="term" value="P:synaptic vesicle transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01369">
        <property type="entry name" value="KISc_KHC_KIF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000067">
        <property type="entry name" value="Kinesin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1590">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968">
        <property type="entry name" value="CENTROMERE PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968:SF36">
        <property type="entry name" value="KINESIN HEAVY CHAIN ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3 10" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7 9 10" id="KW-0505">Motor protein</keyword>
    <keyword evidence="4 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="12">
        <location>
            <begin position="12"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="344"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="429"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="606"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="91"/>
            <end position="98"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000003405.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="860" mass="99057" checksum="F90A93466E4725A4" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>F6UU12</accession>
    <name>F6UU12_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ZP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
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                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
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                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
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                <person name="Doggett N."/>
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                <person name="Richardson P."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="9">wild type</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
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                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
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                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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            <authorList>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
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                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
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                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
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                <person name="Mochizuki Y."/>
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                <person name="Hyatt P.D."/>
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                <person name="Doggett N."/>
                <person name="Glavina T."/>
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                <person name="Richardson P."/>
                <person name="Lucas S."/>
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                <person name="Levine M."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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            <authorList>
                <consortium name="Ensembl"/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
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                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
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                <person name="Hayashi H."/>
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                <person name="Mochizuki Y."/>
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                <person name="Yagi K."/>
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                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
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                <person name="Doggett N."/>
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                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01002412">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6VAL7"/>
    <dbReference type="STRING" id="7719.ENSCINP00000022564"/>
    <dbReference type="Ensembl" id="ENSCINT00000022810.2">
        <property type="protein sequence ID" value="ENSCINP00000022564.2"/>
        <property type="gene ID" value="ENSCING00000007012.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000167607"/>
    <dbReference type="HOGENOM" id="CLU_017594_1_0_1"/>
    <dbReference type="InParanoid" id="F6VAL7"/>
    <dbReference type="OMA" id="IWITNDE"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd16018">
        <property type="entry name" value="Enpp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002591">
        <property type="entry name" value="Phosphodiest/P_Trfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10151">
        <property type="entry name" value="ECTONUCLEOTIDE PYROPHOSPHATASE/PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10151:SF126">
        <property type="entry name" value="ECTONUCLEOTIDE PYROPHOSPHATASE_PHOSPHODIESTERASE FAMILY MEMBER 7-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01663">
        <property type="entry name" value="Phosphodiest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003343666" evidence="1">
        <location>
            <begin position="20"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000022564.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="235" mass="26564" checksum="4F2834AE54900737" modified="2012-04-18" version="2">MQALLRCLLSLAFFSTATTVATKHHKLLLISFDGFRYDYANEEPMFPAFQYLNANGVRAKYLQGAFPTLTSPSHMSIATGLYTESHNVVHNCHYNMSVGHQGSDFLSTLPVNYWWDTGVEPIWIAAVNQGLKSGGYMFPGSYATINGVSATKKVLQKQFMPYSQRSWQNRISTVVGWLKDEDMDFVALYFEEPDTLSHELGPDSKRVRYDVKSWVSNTIMHLLKKVEDAGLTDDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>F6VUH2</accession>
    <accession>A0A1W3JNB9</accession>
    <name>F6VUH2_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Leiomodin-3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">tmod</name>
        <name evidence="9" type="synonym">TMOD</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2012" name="Gene" volume="510" first="78" last="86">
            <title>Actin capping proteins, CapZ (beta-actinin) and tropomodulin in amphioxus striated muscle.</title>
            <authorList>
                <person name="Bao Y."/>
                <person name="Kake T."/>
                <person name="Hanashima A."/>
                <person name="Nomiya Y."/>
                <person name="Kubokawa K."/>
                <person name="Kimura S."/>
            </authorList>
            <dbReference type="PubMed" id="22951807"/>
            <dbReference type="DOI" id="10.1016/j.gene.2012.07.081"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
            <location evidence="6">Myofibril</location>
            <location evidence="6">Sarcomere</location>
            <location evidence="6">M line</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tropomodulin family.</text>
    </comment>
    <dbReference type="EMBL" id="AB736190">
        <property type="protein sequence ID" value="BAM34033.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001265917.1">
        <property type="nucleotide sequence ID" value="NM_001278988.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009861361.1">
        <property type="nucleotide sequence ID" value="XM_009863059.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_026694228.1">
        <property type="nucleotide sequence ID" value="XM_026838427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6VUH2"/>
    <dbReference type="STRING" id="7719.ENSCINP00000000066"/>
    <dbReference type="Ensembl" id="ENSCINT00000000066.3">
        <property type="protein sequence ID" value="ENSCINP00000000066.3"/>
        <property type="gene ID" value="ENSCING00000000042.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100175342"/>
    <dbReference type="KEGG" id="cin:100175342"/>
    <dbReference type="CTD" id="43633"/>
    <dbReference type="GeneTree" id="ENSGT00940000169280"/>
    <dbReference type="HOGENOM" id="CLU_031052_0_1_1"/>
    <dbReference type="OMA" id="PYQRDKL"/>
    <dbReference type="OrthoDB" id="2163268at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031430">
        <property type="term" value="C:M band"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030016">
        <property type="term" value="C:myofibril"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005523">
        <property type="term" value="F:tropomyosin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030239">
        <property type="term" value="P:myofibril assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051694">
        <property type="term" value="P:pointed-end actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000078">
        <property type="entry name" value="Leiomodin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004934">
        <property type="entry name" value="TMOD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10901">
        <property type="entry name" value="TROPOMODULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10901:SF6">
        <property type="entry name" value="TROPOMODULIN, ISOFORM N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03250">
        <property type="entry name" value="Tropomodulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="43"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="54"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00070923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAM34033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000000066.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="366" mass="41627" checksum="90D09E00451C863F" modified="2011-07-27" version="1">MAEVYEFIKQEQEKYLNINEDEILKDLTEDEIEQLTLELEDLDPDNQLLPAGFRQKDQTKKDPTGPLDREKLITYIEEEAKNVEDKEDLVPYEAGIKRGKVFEKTEASEKSLPPSHLDPELEECLNSATEAELTDIAAILGMHTMMNTDQFYSSLMETKIVNKTGYTSTTKCEMKVTALSEPPNPTDVEETLSRIRDNDKSLTAVNLNNIKHIPIHTLVDYCEAIKVNTCVTSWSLASTRTNDSVAFAIADMLKINRTLKVLNVESNFISGNGILAILATLEDNDVLEELRIDNQRAQFGNKVENEMCEILENNKTLLKFGYQFNLQGPRGKVADCLTRNYDTKRKTRVNYNPSSVVSKTFIKKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>F6VXL3</accession>
    <name>F6VXL3_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ATP-dependent DNA helicase</fullName>
            <ecNumber evidence="10">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100187076</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">wild type</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the helicase family. RecQ subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="10"/>
    <dbReference type="EMBL" id="EAAA01000240">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6VXL3"/>
    <dbReference type="FunCoup" id="F6VXL3">
        <property type="interactions" value="505"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000008258"/>
    <dbReference type="Ensembl" id="ENSCINT00000008258.3">
        <property type="protein sequence ID" value="ENSCINP00000008258.3"/>
        <property type="gene ID" value="ENSCING00000004012.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157013"/>
    <dbReference type="InParanoid" id="F6VXL3"/>
    <dbReference type="OMA" id="EYLVAWC"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18794">
        <property type="entry name" value="SF2_C_RecQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:001149">
        <property type="entry name" value="ATP-dependent DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001544">
        <property type="entry name" value="ATP-dependent DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001975">
        <property type="entry name" value="ATP-dependent DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004589">
        <property type="entry name" value="DNA_helicase_ATP-dep_RecQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032284">
        <property type="entry name" value="RecQ_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018982">
        <property type="entry name" value="RQC_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00614">
        <property type="entry name" value="recQ_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710:SF105">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE Q1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710">
        <property type="entry name" value="DNA HELICASE RECQ FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16124">
        <property type="entry name" value="RecQ_Zn_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00956">
        <property type="entry name" value="RQC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 10" id="KW-0347">Helicase</keyword>
    <keyword evidence="4 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="11">
        <location>
            <begin position="101"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="12">
        <location>
            <begin position="301"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU364117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000008258.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="622" mass="71126" checksum="5AA8AE0ADAD2B501" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>F6VZA9</accession>
    <name>F6VZA9_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">LanC-like protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100181052</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LanC-like protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="F6VZA9"/>
    <dbReference type="Ensembl" id="ENSCINT00000003139.3">
        <property type="protein sequence ID" value="ENSCINP00000003139.3"/>
        <property type="gene ID" value="ENSCING00000001571.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063186"/>
    <dbReference type="HOGENOM" id="CLU_036244_0_1_1"/>
    <dbReference type="InParanoid" id="F6VZA9"/>
    <dbReference type="OMA" id="YQEGCGE"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031179">
        <property type="term" value="P:peptide modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04794">
        <property type="entry name" value="euk_LANCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000012">
        <property type="entry name" value="LanC-like protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007822">
        <property type="entry name" value="LANC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020464">
        <property type="entry name" value="LanC-like_prot_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12736">
        <property type="entry name" value="LANC-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12736:SF7">
        <property type="entry name" value="LANC-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05147">
        <property type="entry name" value="LANC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01951">
        <property type="entry name" value="LANCEUKARYTE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01950">
        <property type="entry name" value="LANCSUPER"/>
    </dbReference>
    <dbReference type="SMART" id="SM01260">
        <property type="entry name" value="LANC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
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                <person name="Sasakura Y."/>
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                <person name="Shin-i T."/>
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                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <reaction evidence="8">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
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        <text evidence="2 9">Belongs to the TCP-1 chaperonin family.</text>
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</entry>
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    <reference evidence="10" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">wild type</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="interaction">
        <interactant intactId="EBI-26590857">
            <id>F6WQK5</id>
        </interactant>
        <interactant intactId="EBI-26590938">
            <id>F6WD51</id>
            <label>atf3</label>
        </interactant>
        <organismsDiffer>false</organismsDiffer>
        <experiments>2</experiments>
    </comment>
    <comment type="interaction">
        <interactant intactId="EBI-26590857">
            <id>F6WQK5</id>
        </interactant>
        <interactant intactId="EBI-26590909">
            <id>Q4H3V8</id>
            <label>Ci-ATF4/5</label>
        </interactant>
        <organismsDiffer>false</organismsDiffer>
        <experiments>2</experiments>
    </comment>
    <comment type="interaction">
        <interactant intactId="EBI-26590857">
            <id>F6WQK5</id>
        </interactant>
        <interactant intactId="EBI-26590887">
            <id>F6SWN3</id>
        </interactant>
        <organismsDiffer>false</organismsDiffer>
        <experiments>2</experiments>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bZIP family. C/EBP subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001685">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6WQK5"/>
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    <dbReference type="Ensembl" id="ENSCINT00000023995.1">
        <property type="protein sequence ID" value="ENSCINP00000023749.1"/>
        <property type="gene ID" value="ENSCING00000012799.1"/>
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        <property type="component" value="Chromosome 3"/>
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        <property type="entry name" value="bZIP_CEBPG"/>
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        <property type="entry name" value="bZIP"/>
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        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031106">
        <property type="entry name" value="C/EBP"/>
    </dbReference>
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        <property type="entry name" value="CCAAT/ENHANCER BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23334:SF69">
        <property type="entry name" value="CCAAT_ENHANCER-BINDING PROTEIN GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07716">
        <property type="entry name" value="bZIP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="8">
        <location>
            <begin position="70"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="41"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="155"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="90"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="162"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000023749.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="182" mass="20073" checksum="C65FDCF1D6D0CAA6" modified="2011-07-27" version="1">MNNQNDTGTPSGSGNQDTNVNFGDPGQTAEPLIKFKYPQRPQTVPAVQNFGGKAPPPSKAIVKGELEGDQDDYVKRRQRNNIAVKKSREKSREKSQITSERIDQLKEENCVLENKVEVLNQELKVLKQVFMDHAKGFSGSGIDLPDLEQLEKLLGHKLTDKPGTSQMHDNKTNGPSCSGSKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>F6WRV9</accession>
    <name>F6WRV9_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Folylpolyglutamate synthase</fullName>
            <ecNumber evidence="17">6.3.2.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Folylpoly-gamma-glutamate synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Tetrahydrofolylpolyglutamate synthase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Catalyzes conversion of folates to polyglutamate derivatives allowing concentration of folate compounds in the cell and the intracellular retention of these cofactors, which are important substrates for most of the folate-dependent enzymes that are involved in one-carbon transfer reactions involved in purine, pyrimidine and amino acid synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 17">
            <text>(6S)-5,6,7,8-tetrahydrofolyl-(gamma-L-Glu)(n) + L-glutamate + ATP = (6S)-5,6,7,8-tetrahydrofolyl-(gamma-L-Glu)(n+1) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10580"/>
            <dbReference type="Rhea" id="RHEA-COMP:14738"/>
            <dbReference type="Rhea" id="RHEA-COMP:14740"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:141005"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.17"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>a monovalent cation</name>
            <dbReference type="ChEBI" id="CHEBI:60242"/>
        </cofactor>
        <text evidence="17">A monovalent cation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4 17">Cofactor biosynthesis; tetrahydrofolylpolyglutamate biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 17">Belongs to the folylpolyglutamate synthase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.17" evidence="17"/>
    <dbReference type="AlphaFoldDB" id="F6WRV9"/>
    <dbReference type="FunCoup" id="F6WRV9">
        <property type="interactions" value="138"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000004320"/>
    <dbReference type="Ensembl" id="ENSCINT00000004320.3">
        <property type="protein sequence ID" value="ENSCINP00000004320.3"/>
        <property type="gene ID" value="ENSCING00000002123.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000016526"/>
    <dbReference type="HOGENOM" id="CLU_015869_0_3_1"/>
    <dbReference type="InParanoid" id="F6WRV9"/>
    <dbReference type="OMA" id="ESLDCCM"/>
    <dbReference type="UniPathway" id="UPA00850"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004326">
        <property type="term" value="F:tetrahydrofolylpolyglutamate synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046901">
        <property type="term" value="P:tetrahydrofolylpolyglutamate biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Folylpolyglutamate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Folylpolyglutamate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.20">
        <property type="entry name" value="Mur ligase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="entry name" value="Mur-like, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001645">
        <property type="entry name" value="Folylpolyglutamate_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018109">
        <property type="entry name" value="Folylpolyglutamate_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023600">
        <property type="entry name" value="Folylpolyglutamate_synth_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036615">
        <property type="entry name" value="Mur_ligase_C_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01499">
        <property type="entry name" value="folC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11136:SF5">
        <property type="entry name" value="FOLYLPOLYGLUTAMATE SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11136">
        <property type="entry name" value="FOLYLPOLYGLUTAMATE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038895">
        <property type="entry name" value="FPGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="MurD-like peptide ligases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53244">
        <property type="entry name" value="MurD-like peptide ligases, peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01011">
        <property type="entry name" value="FOLYLPOLYGLU_SYNT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01012">
        <property type="entry name" value="FOLYLPOLYGLU_SYNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 17" id="KW-0436">Ligase</keyword>
    <keyword evidence="13 19" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="10 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 17" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="19">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="305"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="319"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00047493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRNR" id="PIRNR038895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR038895-1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR038895-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000004320.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="500" mass="56455" checksum="049ACE9ACE0D7655" modified="2012-04-18" version="2">DTKYEEAVQTLNELQCYKMLSAKQKLEGLGLLLMKEFLHRVDVSEKDINDLRVIHVTGTKGKGSTCAFVESILRKHGVKTGFYSSPHLLEVRERIRLNGRPISTDLFTKYFWEVHELLKISEDDYEAKIPSYFYMLTILAFYIFKHEKVGAAVIEVGIGGAFDATNVISRPICCGVAALGIDHTEYLGNTIEKIAWQKGGIFKPGTPAFTLPQPTEGMAVLKQRANEFNVDLKIVPNFDSYPRSDDLTLGLSGDHQKLNASLATQLANCWLQRTSMSPIMTSPNLLFPLNEPFRQGLHSCVWNGRGQVIKFRSVSFYLDGAHTVRSLTCATKWFMEESSTEEFHLKTSCEKILAFNVTNNRDAKQFLEILFPCGFSRSLFVPNIASHAPSRFNDQNNLKIPIENQIFRIFANRDAWKEVCQSRGTPTNNDEAQTFLSVADALSSVTEGRDEELWKTLQSMTSQEDYPTMKLRGKHLQVFVTGSLHLVGAFLMILKPDINE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>F6WXI1</accession>
    <name>F6WXI1_CIOIN</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">wild type</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01001736">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6WXI1"/>
    <dbReference type="Ensembl" id="ENSCINT00000019054.2">
        <property type="protein sequence ID" value="ENSCINP00000019054.2"/>
        <property type="gene ID" value="ENSCING00000012703.1"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2195976_0_0_1"/>
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        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000019054.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="108" mass="12892" checksum="8A66A7CB424A128A" modified="2011-07-27" version="1">MGYYKWACLKRSVCTQKQEMTSWKTFKTRTRFLLDCNQRKRETMRFKTSPYYVLLNCTARSDYKEVLKRTNNFQAQRVINFSKTRYNSSSSSDKRSDAARLDFVLPGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>F6WXJ5</accession>
    <accession>A0A1W2WKT7</accession>
    <name>F6WXJ5_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">L-serine ammonia-lyase</fullName>
            <ecNumber evidence="3">4.3.1.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">L-serine deaminase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">L-threonine dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100178137</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-serine = pyruvate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:19169"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="EC" id="4.3.1.17"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the serine/threonine dehydratase family.</text>
    </comment>
    <dbReference type="EC" id="4.3.1.17" evidence="3"/>
    <dbReference type="RefSeq" id="XP_002129370.1">
        <property type="nucleotide sequence ID" value="XM_002129334.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6WXJ5"/>
    <dbReference type="FunCoup" id="F6WXJ5">
        <property type="interactions" value="27"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000019051"/>
    <dbReference type="Ensembl" id="ENSCINT00000019051.3">
        <property type="protein sequence ID" value="ENSCINP00000019051.3"/>
        <property type="gene ID" value="ENSCING00000009373.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100178137"/>
    <dbReference type="KEGG" id="cin:100178137"/>
    <dbReference type="GeneTree" id="ENSGT00600000084626"/>
    <dbReference type="HOGENOM" id="CLU_021152_4_1_1"/>
    <dbReference type="InParanoid" id="F6WXJ5"/>
    <dbReference type="OMA" id="QTQHLGQ"/>
    <dbReference type="OrthoDB" id="4418812at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003941">
        <property type="term" value="F:L-serine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004794">
        <property type="term" value="F:threonine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006565">
        <property type="term" value="P:L-serine catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006567">
        <property type="term" value="P:L-threonine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04886">
        <property type="entry name" value="ACT_ThrD-II-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01562">
        <property type="entry name" value="Thr-dehyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000007">
        <property type="entry name" value="L-threonine dehydratase catabolic TdcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044561">
        <property type="entry name" value="ACT_ThrD-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050147">
        <property type="entry name" value="Ser/Thr_Dehydratase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000634">
        <property type="entry name" value="Ser/Thr_deHydtase_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005789">
        <property type="entry name" value="Thr_deHydtase_catblc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01127">
        <property type="entry name" value="ilvA_1Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48078:SF19">
        <property type="entry name" value="ACT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48078">
        <property type="entry name" value="THREONINE DEHYDRATASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00165">
        <property type="entry name" value="DEHYDRATASE_SER_THR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="9">
        <location>
            <begin position="52"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00041766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00042605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000019051.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="444" mass="48092" checksum="B832CB2745A2821E" modified="2011-07-27" version="1">MSKVNMPENLQTTSRDVDVADDVILDPNCDPDNPVVVDYDRVVDAKQCIGRGVVRTPCTWSRMSHNLGIDIFFKKEFMQFTGSFKDRGALYSLLKLSDEQRRGGVVTTSAGNHAQALAYQGRKLNIPVTVVMPMQAPLVKVNSCKDLQATVILHGQRFDEAKSYAMAYGKKHNLLYINGYDHPDILAGQGTIGLEILEDVPDVDCIVVPIGGGGLVAGIAAAAKHIRPEIKIIGVESQKCPSWTSAVNNGKPLPCPISKQGAKDSITDGLSVIEVGVNSYATAHPLMDKMVTVSEDYVCTAILQLMEFEKSVVEGAGAAGFAAIIAGLLPELHGLKVVTVLCGGNIDITTLGRVIDRALMMQGRLCRFNCIISDRAGGLAEILGIVADGGASIKDIMHDRMSLPSFVYKTSVRLTVETRDKNHARSLREKLEEHYGSDLNWKAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>F6XIM3</accession>
    <name>F6XIM3_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Gonadotropin-releasing hormone receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">gnrhr2</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">wild type</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="EAAA01001754">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001027656.2">
        <property type="nucleotide sequence ID" value="NM_001032484.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6XIM3"/>
    <dbReference type="STRING" id="7719.ENSCINP00000007614"/>
    <dbReference type="Ensembl" id="ENSCINT00000007614.3">
        <property type="protein sequence ID" value="ENSCINP00000007614.3"/>
        <property type="gene ID" value="ENSCING00000003693.3"/>
    </dbReference>
    <dbReference type="GeneID" id="445658"/>
    <dbReference type="KEGG" id="cin:445658"/>
    <dbReference type="CTD" id="114814"/>
    <dbReference type="GeneTree" id="ENSGT00940000166987"/>
    <dbReference type="HOGENOM" id="CLU_009579_15_1_1"/>
    <dbReference type="InParanoid" id="F6XIM3"/>
    <dbReference type="OMA" id="THSFRAD"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032870">
        <property type="term" value="P:cellular response to hormone stimulus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd15195">
        <property type="entry name" value="7tmA_GnRHR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000623">
        <property type="entry name" value="Gonadotropin-releasing hormone receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24241:SF59">
        <property type="entry name" value="ADIPOKINETIC HORMONE RECEPTOR, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24241">
        <property type="entry name" value="NEUROPEPTIDE RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="45"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="79"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="122"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="155"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="208"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="265"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="304"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="8">
        <location>
            <begin position="46"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000007614.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="401" mass="45388" checksum="797743B3B6017E3B" modified="2011-07-27" version="1">MTTAAFTQTYADRIYPSTATNTFLPLCQSHDTILQTWFQFDTLHLVRVLVTWLLFCLSMAGNMFVLWSLRGSKSRHFIMFYLALSDLIYTIFVMPSDAVWNTTMEWLAGDVMCRLCQMMKQFGMYASSFMVVVIGMDRVTAILSPLTHEGQRKRGYCMVLAAWTTSLLCCIPAGALFSLLTVETCEGINVYQCVDFNIVKDRSLLRPYYFFTMCMSFLLPLICTLVSYSLIVCEISNMKERDRVLMGRRKSVNTASIQRAKNRTILMRTLITLTFLVCWGPYYGKGIYDWFQKPAIGPPAPLDTAMYIVMYLNPVLHPIVFGVFMKEIRSKFKKTSCIAGLKQKREQVRSGSVSRLSLTSYATGLTQVQDLPNGAAETTVLTGATQQVPSIDESVHTKAPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F6XIV1</accession>
    <name>F6XIV1_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">wild type</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01001603">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6XIV1"/>
    <dbReference type="STRING" id="7719.ENSCINP00000021694"/>
    <dbReference type="Ensembl" id="ENSCINT00000021940.2">
        <property type="protein sequence ID" value="ENSCINP00000021694.2"/>
        <property type="gene ID" value="ENSCING00000011323.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000016768"/>
    <dbReference type="HOGENOM" id="CLU_1820128_0_0_1"/>
    <dbReference type="InParanoid" id="F6XIV1"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024868">
        <property type="entry name" value="FJX1/FJ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13147">
        <property type="entry name" value="FOUR-JOINTED BOX PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13147:SF5">
        <property type="entry name" value="FOUR-JOINTED BOX PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02072">
        <property type="entry name" value="4JOINTEDBOX1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000021694.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="142" mass="16699" checksum="31E823CBC043EC0F" modified="2012-04-18" version="2">KNLEAAGWRPLAVVSITLFIPDLQTVEFPPYLKDCINCKLDKEYNEELTPEESNFLTQWSDMILLDYVTGDVDRVIGHVHNLKWDDRSFNRPVHNLMKDQEGSLYFFDNESAFGHSYRLLARYQTLHDVTLKRVCVFSRRTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>F6XPL3</accession>
    <name>F6XPL3_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">wild type</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Microtubule-based anterograde translocator for membranous organelles. Plus end-directed microtubule sliding activity in vitro. Plays a role in primary cilia formation. Plays a role in centriole cohesion and subdistal appendage organization and function. Regulates the formation of the subdistal appendage via recruitment of DCTN1 to the centriole. Also required for ciliary basal feet formation and microtubule anchoring to mother centriole.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Heterodimer of KIF3A and KIF3B. Interacts with CIMAP3. Interacts with CLN3. Interacts with DCTN1. Interacts with FLCN. Interacts with AP3B1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11 12">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001464">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6XPL3"/>
    <dbReference type="FunCoup" id="F6XPL3">
        <property type="interactions" value="219"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000017449"/>
    <dbReference type="Ensembl" id="ENSCINT00000017449.3">
        <property type="protein sequence ID" value="ENSCINP00000017449.3"/>
        <property type="gene ID" value="ENSCING00000008554.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156386"/>
    <dbReference type="HOGENOM" id="CLU_001485_22_3_1"/>
    <dbReference type="InParanoid" id="F6XPL3"/>
    <dbReference type="OMA" id="NMRKHIE"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904115">
        <property type="term" value="C:axon cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005871">
        <property type="term" value="C:kinesin complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016939">
        <property type="term" value="C:kinesin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008089">
        <property type="term" value="P:anterograde axonal transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002244">
        <property type="term" value="P:hematopoietic progenitor cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007219">
        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045494">
        <property type="term" value="P:photoreceptor cell maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035845">
        <property type="term" value="P:photoreceptor cell outer segment organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd01371">
        <property type="entry name" value="KISc_KIF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000028">
        <property type="entry name" value="Kinesin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969">
        <property type="entry name" value="CHROMOSOME-ASSOCIATED KINESIN KIF4A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969:SF21">
        <property type="entry name" value="KINESIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3 12" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7 11 12" id="KW-0505">Motor protein</keyword>
    <keyword evidence="4 11 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="14">
        <location>
            <begin position="8"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="399"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="413"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="93"/>
            <end position="100"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00058965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00065526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
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                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    <dbReference type="AlphaFoldDB" id="F6YS42"/>
    <dbReference type="Ensembl" id="ENSCINT00000026968.2">
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    <sequence length="427" mass="48783" checksum="EC9B327F6FD6F2B6" modified="2012-04-18" version="2">MGGRTDSQCVKRFNTIKRAVSMGRALIYNPSTTYSNNVHKIRKTLLEKISAETTPVKTEKDNDNDKNKDKENGDDGPPEPDAPTDTPHKHLIKISSKPKQKVLPAQDALLNMSSNLSSREVLELYIELMKTRRGRRGGKMGIAGKMNHEDIQINFELKKFLEERYKPMKRQSRKLENDNLGFCLKEITKNSKSISSKDIFIYYIKALNINAARALESIRIKKDKQREGKVTLRSVLRNEIEAGNKAVGEMRKKMSKRNADSLTSSDITSDNTDITKQQSTSNKADSDVTNKSRAFARNLYLPLCAPNRATTKAYYALVKDRERRVTQAKTILRLDDATDETSGGKFKTVDLLADMRARYDYKVFRSRFNALFTWSTFLRSLVPYRRTGNDRVLRPAIRIKRPYYDVTIPSAKRHKPNDSTIDNESSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
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    <reference evidence="7" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
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                <person name="Hastings K.E."/>
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                <person name="Meinertzhagen I.A."/>
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                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
    </reference>
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            <location evidence="1">Cytoplasm</location>
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    <comment type="similarity">
        <text evidence="2">Belongs to the MLF family.</text>
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    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
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    <name>F6ZLC8_CIOIN</name>
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    <reference evidence="6" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
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                <person name="Hastings K.E."/>
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                <person name="Makabe K.W."/>
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                <person name="Mochizuki Y."/>
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                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
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                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
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                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">wild type</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
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    <dbReference type="EMBL" id="EAAA01001633">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6ZLC8"/>
    <dbReference type="STRING" id="7719.ENSCINP00000010460"/>
    <dbReference type="Ensembl" id="ENSCINT00000010460.3">
        <property type="protein sequence ID" value="ENSCINP00000010460.3"/>
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    <dbReference type="InParanoid" id="F6ZLC8"/>
    <dbReference type="OMA" id="CESTHTC"/>
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        <property type="term" value="F:calcium ion binding"/>
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        <location>
            <begin position="182"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="193"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
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    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="238" mass="26095" checksum="A1FA2ED8768C063B" modified="2012-04-18" version="2">MENIHSFACSVCSDMDCEDCYNSAHCHWVPGNVTSHNKNGYCEENTNNTTQVKKTAPLAIHNYTMCFQQCSIFTSCNTCLSNYDDISVCLWSTQVNKCMSVVESILQCSAGLCGHVVQLGSQCPTPCSYFTYCNHCLLNEGCGWVGNDDGSGHGECTAGTYLAPIDLQAVDSSQWWFISCPQENECLNGHASCQNQDNEDCQDTHDAFECVCAPGYTRDNITLLCKPHCQSGCVKGDC</sequence>
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<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>F6ZR39</accession>
    <name>F6ZR39_CIOIN</name>
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        <recommendedName>
            <fullName evidence="1">Sulfotransferase</fullName>
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        <lineage>
            <taxon>Eukaryota</taxon>
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    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sulfotransferase 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="1"/>
    <dbReference type="EMBL" id="EAAA01001711">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6ZR39"/>
    <dbReference type="STRING" id="7719.ENSCINP00000023662"/>
    <dbReference type="Ensembl" id="ENSCINT00000023908.2">
        <property type="protein sequence ID" value="ENSCINP00000023662.2"/>
        <property type="gene ID" value="ENSCING00000012751.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164976"/>
    <dbReference type="HOGENOM" id="CLU_028381_4_0_1"/>
    <dbReference type="InParanoid" id="F6ZR39"/>
    <dbReference type="OMA" id="VETECYE"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001517">
        <property type="term" value="F:N-acetylglucosamine 6-O-sulfotransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006044">
        <property type="term" value="P:N-acetylglucosamine metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006790">
        <property type="term" value="P:sulfur compound metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051135">
        <property type="entry name" value="Gal/GlcNAc/GalNAc_ST"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10704">
        <property type="entry name" value="CARBOHYDRATE SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10704:SF71">
        <property type="entry name" value="CARBOHYDRATE SULFOTRANSFERASE 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
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        <location>
            <begin position="23"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU361155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000023662.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="203" mass="23454" checksum="1969C98E8DC94933" modified="2012-04-18" version="2">MVAMKVIYLCRLEWITPLLLSKQSIVKIVHLVRDPRAIINSRIPQRETNSAVNKARFGVIRFYAHQICDQLSSNVGYADTVVGSIVPRKSYLRIRHEDFALEPIQVSENIYKFLSLPFREGMRRWVLDATTSNTQTSELKTGDQGTKRNSKEIVSAWRGKLSFEEVDVIQEICKDSLNKLGYKIFNTEKELQNVQELHFKPKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
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    <gene>
        <name evidence="15" type="primary">LOC100182530</name>
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    <organism evidence="15 16">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
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    <reference evidence="16" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">wild type</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine and L-5-hydroxytryptophan to serotonin.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 13 14">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="9">Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the group II decarboxylase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.28" evidence="10"/>
    <dbReference type="EMBL" id="EAAA01002073">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009858556.1">
        <property type="nucleotide sequence ID" value="XM_009860254.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_026690313.1">
        <property type="nucleotide sequence ID" value="XM_026834512.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6ZTZ7"/>
    <dbReference type="FunCoup" id="F6ZTZ7">
        <property type="interactions" value="4"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000019588"/>
    <dbReference type="Ensembl" id="ENSCINT00000019588.3">
        <property type="protein sequence ID" value="ENSCINP00000019588.3"/>
        <property type="gene ID" value="ENSCING00000009636.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100182530"/>
    <dbReference type="KEGG" id="cin:100182530"/>
    <dbReference type="GeneTree" id="ENSGT00940000156004"/>
    <dbReference type="HOGENOM" id="CLU_011856_3_1_1"/>
    <dbReference type="InParanoid" id="F6ZTZ7"/>
    <dbReference type="OMA" id="IPFEMEY"/>
    <dbReference type="OrthoDB" id="639767at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004058">
        <property type="term" value="F:aromatic-L-amino-acid decarboxylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042423">
        <property type="term" value="P:catecholamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006584">
        <property type="term" value="P:catecholamine metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042427">
        <property type="term" value="P:serotonin biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06450">
        <property type="entry name" value="DOPA_deC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000025">
        <property type="entry name" value="Histidine decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1340.10">
        <property type="entry name" value="dopa decarboxylase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010977">
        <property type="entry name" value="Aromatic_deC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002129">
        <property type="entry name" value="PyrdxlP-dep_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021115">
        <property type="entry name" value="Pyridoxal-P_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11999:SF167">
        <property type="entry name" value="AROMATIC-L-AMINO-ACID DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11999">
        <property type="entry name" value="GROUP II PYRIDOXAL-5-PHOSPHATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00282">
        <property type="entry name" value="Pyridoxal_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00800">
        <property type="entry name" value="YHDCRBOXLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00392">
        <property type="entry name" value="DDC_GAD_HDC_YDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0127">Catecholamine biosynthesis</keyword>
    <keyword evidence="5" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="7 14" id="KW-0456">Lyase</keyword>
    <keyword evidence="6 13 14" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="13">
        <location>
            <position position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00040968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00041275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR602129-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000019588.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="492" mass="55814" checksum="C136515C05807822" modified="2012-04-18" version="2">MDGNNSDEEPRLGIEPETFRHAATNMVDYIINYHRDIHKRQTFPDVEPGFMQARLPKEAPDYPESWQEVFSDIETVVMDGMTHWQSPGFFSYYPATTSYPSMLADMLCNGISCVRFSWASSPSATELETVMMDWLAKAIGLPECFIHGGHGPGGGVIQGSASESTLMALMAARNKTIRQELSRDKSLRTHDIVARMVAYSSQCTHSCMDRAGVFALVEVRKLPVGKDGVMRGSVLKEAVMKDKDDGRIPMFVCASIGTTPCCTFDDLEEIGKICEEQEIWCHVDAAYAGAALICPEFRYICKGVERVTSFNFNPHKWLMVQIDCSAMWVRNSDDLINSAEVNPLFLHHKAQDSAIDYRHWQIPLGRPFRSLKLWFVLRMVGIEGLRSNIRRGVQEAKHLERLIRSDERFEILFPVTLGLVCFKFKHPGLLLEEENSLNERLYQKIHNDKRILLVLAMVNGVYFIRVCTGSTHCSIAQVNKCWNVIKEMAEQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F6ZV67</accession>
    <name>F6ZV67_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="F6ZV67"/>
    <dbReference type="Ensembl" id="ENSCINT00000026682.2">
        <property type="protein sequence ID" value="ENSCINP00000026436.2"/>
        <property type="gene ID" value="ENSCING00000014690.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000168786"/>
    <dbReference type="HOGENOM" id="CLU_1795796_0_0_1"/>
    <dbReference type="InParanoid" id="F6ZV67"/>
    <dbReference type="OMA" id="NRTEEYH"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038826">
        <property type="entry name" value="CCDC178"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35088">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 178"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35088:SF1">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 178"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="8"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000026436.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="144" mass="16785" checksum="8DF502490281E337" modified="2012-04-18" version="2">MSQRLEVKTNLEENVKEEMEKNKMLAQRYRELQSEHIDLKNRLMDLYDSKVKLETSIKDHKQLVTLQEKLNVALKQYYHDRGEFNKAGLEKFHVRSHDNTDKMSKVQGGLEVALDNISLFLKSQIDGSAANRVHQAAMDWLTTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>F6ZX85</accession>
    <name>F6ZX85_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">VWFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
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    <dbReference type="AlphaFoldDB" id="F7A6L3"/>
    <dbReference type="Ensembl" id="ENSCINT00000010188.3">
        <property type="protein sequence ID" value="ENSCINP00000010188.3"/>
        <property type="gene ID" value="ENSCING00000004950.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165506"/>
    <dbReference type="HOGENOM" id="CLU_016785_9_3_1"/>
    <dbReference type="InParanoid" id="F7A6L3"/>
    <dbReference type="OMA" id="INIWWRS"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016706">
        <property type="term" value="F:2-oxoglutarate-dependent dioxygenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="Lysine-specific demethylase 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041667">
        <property type="entry name" value="Cupin_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12461">
        <property type="entry name" value="HYPOXIA-INDUCIBLE FACTOR 1 ALPHA INHIBITOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12461:SF42">
        <property type="entry name" value="JMJC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13621">
        <property type="entry name" value="Cupin_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="JmjC" evidence="3">
        <location>
            <begin position="104"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="313"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000010188.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="371" mass="43084" checksum="1ECED89894C6768B" modified="2012-04-18" version="2">MQPIGSHMPPMPIEERDDLPNPIEFYDNYVKPGVPVVFKGIAKKFPNFNDMKSDEYLREKHGDFEVLVETAKKEDRNNPPRTMNLSNYLSIYEKEDVYVVQTMFPPEAFTKEAFVPKCLLCQGFLDRLNMVIMWLSSGGTKSVLHNDSFENLNCVLDGTKEFVMIDRKHADLVPIDNPDRGFSSVDVEKVDMHKYPTLGKLPWYIANMEAGDCFYIPKNWFHHVNSSQTRNLAINIWWRSSKEFKHREECEKSLADIPEYDTLWNYRLNNGIKIEDLVFADAFSENGTTTLINLLKITYKTLPKEMEAKQKREALEKLQELFYQADANKDKLLSKDEITSTASSLVQDFLTDEAVLEEEVDELYDEVKEEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>F7AEN4</accession>
    <name>F7AEN4_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Transforming growth factor beta superfamily signaling ligand</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">nodal</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the TGF-beta family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_026693618.1">
        <property type="nucleotide sequence ID" value="XM_026837817.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F7AEN4"/>
    <dbReference type="STRING" id="7719.ENSCINP00000002425"/>
    <dbReference type="Ensembl" id="ENSCINT00000002425.3">
        <property type="protein sequence ID" value="ENSCINP00000002425.3"/>
        <property type="gene ID" value="ENSCING00000001251.3"/>
    </dbReference>
    <dbReference type="GeneID" id="778979"/>
    <dbReference type="CTD" id="4838"/>
    <dbReference type="GeneTree" id="ENSGT00940000160223"/>
    <dbReference type="HOGENOM" id="CLU_672592_0_0_1"/>
    <dbReference type="InParanoid" id="F7AEN4"/>
    <dbReference type="OMA" id="ILYYEDG"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007178">
        <property type="term" value="P:cell surface receptor protein serine/threonine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd13759">
        <property type="entry name" value="TGF_beta_NODAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.90.10:FF:000026">
        <property type="entry name" value="Nodal homolog 3-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001839">
        <property type="entry name" value="TGF-b_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015615">
        <property type="entry name" value="TGF-beta-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017948">
        <property type="entry name" value="TGFb_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848">
        <property type="entry name" value="TGF-BETA FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848:SF302">
        <property type="entry name" value="TGF-BETA FAMILY PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00019">
        <property type="entry name" value="TGF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00204">
        <property type="entry name" value="TGFB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00250">
        <property type="entry name" value="TGF_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51362">
        <property type="entry name" value="TGF_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 10" id="KW-0339">Growth factor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5030169805" evidence="12">
        <location>
            <begin position="22"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="domain" description="TGF-beta family profile" evidence="13">
        <location>
            <begin position="293"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="192"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="198"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000002425.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="415" mass="47884" checksum="AC4E0BC3C1120BA1" modified="2011-07-27" version="1">MISMFNIAAFVFTMTSIFVNGESVTSSNPTNAEALLRHMQQRSNAVRSKYMMELYRAKNTGMPTNNALKRSDIVRSFRSIESYKVGSKLVVVFDLSSVPRERIRGSELQVDRSLSDVTMIELWEGINAESRRKIGTMRYIRTNKSFDTTSQIRRWHRGDSPVTPFHASGAILPLTPLLVVYSNNHHYDLIEASESRMNRQRRSSRGRHSNKYRKRRYRVNKNKTRKSTLRHNDVEITSHAQQKSRRISNDFELSTSSADDVTIQSGNEYDVTDEWRKQQSDDVKVVKAKHPVSPQRKHPFIISTPAPTVGATHCRKVSFEIDFEKIGWGSWIVYPKKYNAYRCEGQCRTPMRSDSKPTNHAYMQSIIATKRPDLHIPQPCCVPTKLKPLSILYYEDGEVKQRDHEDMVVDECGCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>F7AZ47</accession>
    <name>F7AZ47_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="31">Notch</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="31" type="primary">n</name>
    </gene>
    <organism evidence="31 32">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="32" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="31" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="31">wild type</strain>
        </source>
    </reference>
    <reference evidence="31" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="31" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NOTCH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="26">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002966">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="F7AZ47">
        <property type="interactions" value="17"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000007252"/>
    <dbReference type="Ensembl" id="ENSCINT00000007252.3">
        <property type="protein sequence ID" value="ENSCINP00000007252.3"/>
        <property type="gene ID" value="ENSCING00000003521.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00980000198606"/>
    <dbReference type="HOGENOM" id="CLU_000576_0_0_1"/>
    <dbReference type="InParanoid" id="F7AZ47"/>
    <dbReference type="OMA" id="TCHEQRD"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098588">
        <property type="term" value="C:bounding membrane of organelle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0030855">
        <property type="term" value="P:epithelial cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048732">
        <property type="term" value="P:gland development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051241">
        <property type="term" value="P:negative regulation of multicellular organismal process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0050877">
        <property type="term" value="P:nervous system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007219">
        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902895">
        <property type="term" value="P:positive regulation of miRNA transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051240">
        <property type="term" value="P:positive regulation of multicellular organismal process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003002">
        <property type="term" value="P:regionalization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050793">
        <property type="term" value="P:regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="25"/>
    </dbReference>
    <dbReference type="CDD" id="cd21701">
        <property type="entry name" value="JMTM_Notch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000185">
        <property type="entry name" value="basement membrane-specific heparan sulfate proteoglycan core protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000123">
        <property type="entry name" value="Crumbs homolog 1 (Drosophila)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000246">
        <property type="entry name" value="EGF-like repeat and discoidin I-like domain-containing protein 3"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="1.25.40.20:FF:000005">
        <property type="entry name" value="Neurogenic locus notch 1"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="2.10.25.10:FF:000004">
        <property type="entry name" value="Neurogenic locus notch 1"/>
        <property type="match status" value="7"/>
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    <dbReference type="FunFam" id="3.30.300.320:FF:000001">
        <property type="entry name" value="Neurogenic locus notch 1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="neurogenic locus notch homolog protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000060">
        <property type="entry name" value="Neurogenic locus notch protein 1"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="2.10.25.10:FF:000173">
        <property type="entry name" value="Neurogenic locus notch protein 2"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="2.10.25.10:FF:000125">
        <property type="entry name" value="Neurogenic locus notch protein-like"/>
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    <dbReference type="FunFam" id="2.10.25.10:FF:000109">
        <property type="entry name" value="Notch homolog 4, [Drosophila]"/>
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        <property type="entry name" value="Notch, isoform B"/>
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    <dbReference type="FunFam" id="2.10.25.10:FF:000434">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="2.10.25.10:FF:000143">
        <property type="entry name" value="Protein crumbs 1"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="Protein crumbs homolog 2"/>
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        <property type="entry name" value="protein delta homolog 2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Putative neurogenic locus notch"/>
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    <dbReference type="FunFam" id="2.10.25.10:FF:000373">
        <property type="entry name" value="sushi, nidogen and EGF-like domain-containing protein 1"/>
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    <dbReference type="FunFam" id="2.10.25.10:FF:000006">
        <property type="entry name" value="Versican core protein-like isoform 1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="33"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013032">
        <property type="entry name" value="EGF-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008297">
        <property type="entry name" value="Notch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035993">
        <property type="entry name" value="Notch-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR051355">
        <property type="entry name" value="Notch/Slit_guidance"/>
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    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024600">
        <property type="entry name" value="Notch_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000800">
        <property type="entry name" value="Notch_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010660">
        <property type="entry name" value="Notch_NOD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011656">
        <property type="entry name" value="Notch_NODP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45836:SF23">
        <property type="entry name" value="NEUROGENIC LOCUS NOTCH HOMOLOG PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45836">
        <property type="entry name" value="SLIT HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="18"/>
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    <dbReference type="Pfam" id="PF07645">
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        <property type="match status" value="1"/>
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        <property type="match status" value="8"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="3"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00010">
        <property type="entry name" value="EGFBLOOD"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01452">
        <property type="entry name" value="LNOTCHREPEAT"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01983">
        <property type="entry name" value="NOTCH"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="6"/>
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    <dbReference type="SMART" id="SM01334">
        <property type="entry name" value="DUF3454"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="35"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="29"/>
    </dbReference>
    <dbReference type="SMART" id="SM00004">
        <property type="entry name" value="NL"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM01338">
        <property type="entry name" value="NOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01339">
        <property type="entry name" value="NODP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="24"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="3"/>
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    <dbReference type="SUPFAM" id="SSF90193">
        <property type="entry name" value="Notch domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="4"/>
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    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="4"/>
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    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="21"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="34"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="27"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="34"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50258">
        <property type="entry name" value="LNR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="18" id="KW-0010">Activator</keyword>
    <keyword evidence="15 25" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="23" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="11" id="KW-0221">Differentiation</keyword>
    <keyword evidence="17 24 26" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 26" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="21" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="23" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0914">Notch signaling pathway</keyword>
    <keyword evidence="22" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-0675">Receptor</keyword>
    <keyword evidence="32" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 28" id="KW-0732">Signal</keyword>
    <keyword evidence="19" id="KW-0804">Transcription</keyword>
    <keyword evidence="14" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="28">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003348901" evidence="28">
        <location>
            <begin position="26"/>
            <end position="2549"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="29">
        <location>
            <begin position="24"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="29">
        <location>
            <begin position="63"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="29">
        <location>
            <begin position="141"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="29">
        <location>
            <begin position="179"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="29">
        <location>
            <begin position="224"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="29">
        <location>
            <begin position="263"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="29">
        <location>
            <begin position="301"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="29">
        <location>
            <begin position="341"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="29">
        <location>
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</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>F7B2X5</accession>
    <accession>A0A1W2W8E1</accession>
    <name>F7B2X5_CIOIN</name>
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            <fullName evidence="15">Vesicular integral-membrane protein VIP36</fullName>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
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    <reference evidence="16" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Endomembrane system</location>
            <topology evidence="11">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_002126496.1">
        <property type="nucleotide sequence ID" value="XM_002126460.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F7B2X5"/>
    <dbReference type="FunCoup" id="F7B2X5">
        <property type="interactions" value="495"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000005331"/>
    <dbReference type="Ensembl" id="ENSCINT00000005331.3">
        <property type="protein sequence ID" value="ENSCINP00000005331.3"/>
        <property type="gene ID" value="ENSCING00000002618.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100176828"/>
    <dbReference type="KEGG" id="cin:100176828"/>
    <dbReference type="GeneTree" id="ENSGT00940000155596"/>
    <dbReference type="HOGENOM" id="CLU_041093_0_0_1"/>
    <dbReference type="InParanoid" id="F7B2X5"/>
    <dbReference type="OMA" id="GCTADIR"/>
    <dbReference type="OrthoDB" id="270293at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
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    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
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        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment"/>
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    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
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    <dbReference type="GO" id="GO:0005537">
        <property type="term" value="F:D-mannose binding"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06901">
        <property type="entry name" value="lectin_VIP36_VIPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000017">
        <property type="entry name" value="Vesicular integral-membrane protein VIP36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051136">
        <property type="entry name" value="Intracellular_Lectin-GPT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005052">
        <property type="entry name" value="Lectin_leg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035664">
        <property type="entry name" value="VIP36_lectin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12223:SF45">
        <property type="entry name" value="RE50040P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12223">
        <property type="entry name" value="VESICULAR MANNOSE-BINDING LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03388">
        <property type="entry name" value="Lectin_leg-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51328">
        <property type="entry name" value="L_LECTIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5" id="KW-0430">Lectin</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0732">Signal</keyword>
    <keyword evidence="2 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014090653" evidence="13">
        <location>
            <begin position="27"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="306"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="L-type lectin-like" evidence="14">
        <location>
            <begin position="37"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00046288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000005331.3"/>
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    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="340" mass="38211" checksum="A4BFADD2F4604E10" modified="2012-04-18" version="2">MERCRIFYKFSINIIVFLCLLVASDGQDSSQMAPEGGFLKREYSLTKPYSSNGMTMPFWDFRDRTMLTNNFIRLTSNHQAEVGSVWNKIPLYLRDWEMQVQFKVHGGGRTLGADGFAIWYTKERMTPGPVFGSRDEFHGLGVFFDTYKNGPQAVTFPQITAMVGNGSVKYDHMTDGKSNAIGSCLASFRNKDYDTLVSIKYVKETLQIRLDIDGLGEWRECFSMPGVKLPTGYFIGMSAATGDLADNHDLINVKFFHMDAGRSAEEEAEDWSTVLPGVSYLKPPIDNRDDVGSGDFRNSSVSGFKLFLLVVCILLGVGVCVVVGVVVFQKRQAQNRKRFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>F7B3X8</accession>
    <name>F7B3X8_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cyclin-C</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Binds to and activates cyclin-dependent kinase CDK8 that phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cyclin family. Cyclin C subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="F7B3X8"/>
    <dbReference type="FunCoup" id="F7B3X8">
        <property type="interactions" value="602"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000005055"/>
    <dbReference type="Ensembl" id="ENSCINT00000005055.3">
        <property type="protein sequence ID" value="ENSCINP00000005055.3"/>
        <property type="gene ID" value="ENSCING00000002487.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155625"/>
    <dbReference type="HOGENOM" id="CLU_034754_1_1_1"/>
    <dbReference type="InParanoid" id="F7B3X8"/>
    <dbReference type="OMA" id="CLLHPPH"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990508">
        <property type="term" value="C:CKM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd20513">
        <property type="entry name" value="CYCLIN_CCNC_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20514">
        <property type="entry name" value="CYCLIN_CCNC_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000015">
        <property type="entry name" value="Putative cyclin-c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000017">
        <property type="entry name" value="Putative cyclin-c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043198">
        <property type="entry name" value="Cyclin/Ssn8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031658">
        <property type="entry name" value="Cyclin_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10026">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16899">
        <property type="entry name" value="Cyclin_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028758">
        <property type="entry name" value="Cyclin, C/H/G types"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0195">Cyclin</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Cyclin-like" evidence="12">
        <location>
            <begin position="46"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="12">
        <location>
            <begin position="157"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="266"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="273"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000005055.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="285" mass="32967" checksum="3F1B13F48F46F2EE" modified="2012-04-18" version="2">MAGNFWQSSHCKQWILSPEFLVRERELDLQVMGEVDYQKLMMFFANVIQSIGEQLKLRQQVIATATVYFKRFYSKHSLNSCDPLLLAPTCIFLSSKVEEFGVISNSRLISVVTTVIKSKYSYAFPNEFNYRIHHVWECEFYLLELMDCCLVVFHPYRPLVQYVNALGMADALLPIAWRIANDSLRTDVILLYPPFQIALACLHMACVVQNQEAATFQWFADLNVDMEKIIEITNLILKLYELWKAFDEKSEIPGLLKKMPKVLLSQQRDGKNDSNSGRNNVSVKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>F7BFC8</accession>
    <name>F7BFC8_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="F7BFC8"/>
    <dbReference type="STRING" id="7719.ENSCINP00000011018"/>
    <dbReference type="Ensembl" id="ENSCINT00000011018.3">
        <property type="protein sequence ID" value="ENSCINP00000011018.3"/>
        <property type="gene ID" value="ENSCING00000005366.3"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_863191_0_0_1"/>
    <dbReference type="InParanoid" id="F7BFC8"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005221">
        <property type="term" value="F:intracellularly cyclic nucleotide-activated monoatomic cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050866">
        <property type="entry name" value="CNG_cation_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45638">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-GATED CATION CHANNEL SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45638:SF11">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-GATED CATION CHANNEL SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0407">Ion channel</keyword>
    <keyword evidence="1" id="KW-0406">Ion transport</keyword>
    <keyword evidence="1" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="3">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="141"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="220"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="221"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="250"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000011018.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="322" mass="36138" checksum="9B16672A89E2EAF1" modified="2012-04-18" version="2">MNLSSSTLRRLIGKLERLVYLPGQFVYKSGEPAREVYIVGSGVLQNIREIDGHVNLVLDGEIFGEENFLSMHQTLKRSSNVTACGYTELYMLRAADVIEVLNLEVSSHKSIGSTAKKSTAKTQSISGVDVSLESTRYTTITQSDDRSRWTHSSSPDSALPPSPGRKQEEELKLPKNIGLIVRPDIANKLNLIYQQNKEHLKYSLDTFWNQEISTFQQQLTETDKQRDRRVNDVIRDKDDVTENDVTTDPSTSSAESLATSEKKQNNLFTRTVTYSGKNRKRTVLPVSATNSTKSNSKYRRPSAIQETPLDVYGSLVSLNTLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>F7BGU9</accession>
    <name>F7BGU9_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">wild type</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01000473">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="EAAA01000474">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F7BGU9"/>
    <dbReference type="STRING" id="7719.ENSCINP00000026656"/>
    <dbReference type="Ensembl" id="ENSCINT00000026902.2">
        <property type="protein sequence ID" value="ENSCINP00000026656.2"/>
        <property type="gene ID" value="ENSCING00000021742.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154894"/>
    <dbReference type="HOGENOM" id="CLU_1044002_0_0_1"/>
    <dbReference type="InParanoid" id="F7BGU9"/>
    <dbReference type="OMA" id="WVCAIEA"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015701">
        <property type="term" value="P:bicarbonate transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051453">
        <property type="term" value="P:regulation of intracellular pH"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.930.10">
        <property type="entry name" value="Mannitol-specific EII, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016152">
        <property type="entry name" value="PTrfase/Anion_transptr"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55804">
        <property type="entry name" value="Phoshotransferase/anion transport protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000026656.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="267" mass="30909" checksum="2EF6F00583FDC428" modified="2012-04-18" version="2">MRRRIRQQQQSSSTRETVNSVNYDDVISAERLTEEVGEHKMIPMSNLSVNSLVQNRRQSAGNFRRRMSRDDLQDEEDEFYHRELILKIRKTLPLKNFRDEIRARRDLEKFLVQPVVVLDVPHTSLDKIVDELLSKLQKDLDVDEKITSQAGKSFFTFQETYQDAMASVDHLKETIQGVSYKPDGADYEETWITALCTLPMVNKSHVAIARLRNPLNLGNKCEEVQFVIAIIAPKKEKFTKNAVEIGRTFSSMMCNRGFRHSLMVAET</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>H2XJS6</accession>
    <name>H2XJS6_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Hemocyanin B chain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100183593</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">wild type</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Melanosome membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the tyrosinase family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01000692">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001029009.2">
        <property type="nucleotide sequence ID" value="NM_001033837.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XJS6"/>
    <dbReference type="STRING" id="7719.ENSCINP00000029908"/>
    <dbReference type="Ensembl" id="ENSCINT00000037124.1">
        <property type="protein sequence ID" value="ENSCINP00000029908.1"/>
        <property type="gene ID" value="ENSCING00000018886.1"/>
    </dbReference>
    <dbReference type="GeneID" id="100183593"/>
    <dbReference type="GeneTree" id="ENSGT00940000165243"/>
    <dbReference type="HOGENOM" id="CLU_342867_0_0_1"/>
    <dbReference type="InParanoid" id="H2XJS6"/>
    <dbReference type="OMA" id="YMLEMDK"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042438">
        <property type="term" value="P:melanin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1280.10">
        <property type="entry name" value="Di-copper center containing domain from catechol oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1520">
        <property type="entry name" value="Hemocyanin, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008922">
        <property type="entry name" value="Di-copper_centre_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013788">
        <property type="entry name" value="Hemocyanin/hexamerin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000896">
        <property type="entry name" value="Hemocyanin/hexamerin_mid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005203">
        <property type="entry name" value="Hemocyanin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037020">
        <property type="entry name" value="Hemocyanin_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002227">
        <property type="entry name" value="Tyrosinase_Cu-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11511">
        <property type="entry name" value="LARVAL STORAGE PROTEIN/PHENOLOXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11511:SF4">
        <property type="entry name" value="PHENOLOXIDASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03723">
        <property type="entry name" value="Hemocyanin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00372">
        <property type="entry name" value="Hemocyanin_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00187">
        <property type="entry name" value="HAEMOCYANIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48056">
        <property type="entry name" value="Di-copper centre-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00498">
        <property type="entry name" value="TYROSINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0470">Melanin biosynthesis</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Tyrosinase copper-binding" evidence="9">
        <location>
            <begin position="236"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="23"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS00498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000029908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="702" mass="81075" checksum="1677F3164C7F12A4" modified="2012-04-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>H2XJU6</accession>
    <name>H2XJU6_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01002869">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XJU6"/>
    <dbReference type="Ensembl" id="ENSCINT00000030545.1">
        <property type="protein sequence ID" value="ENSCINP00000029928.1"/>
        <property type="gene ID" value="ENSCING00000021426.1"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1368975_0_0_1"/>
    <dbReference type="InParanoid" id="H2XJU6"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="13"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="179"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="179"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000029928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="200" mass="22861" checksum="C0E66E84F424F55A" modified="2012-04-18" version="1">MQIFREKHVFKLRGRRKKRIPSSENSDSTSDEIAHLPQNKGTRPKLIPLSMADLISNSLAKSSDYNLVDQKTGVSRIKTPSKGYWSDHDAYTCVPQKKSGQSLAPINYKTFTGAHRPTRSYRQESPLVGETWLADLPTNGLEKNKYKFIPTQLRTQPLNLPKPVMSRHRLESLTQLDLRHDSSKKESKIQHEMTSLFQSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>H2XK95</accession>
    <accession>A0A1W2WE48</accession>
    <name>H2XK95_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ribonuclease P protein subunit p30</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">RNase P subunit 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100177882</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Component of ribonuclease P, a ribonucleoprotein complex that generates mature tRNA molecules by cleaving their 5'-ends. Also a component of the MRP ribonuclease complex, which cleaves pre-rRNA sequences.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of nuclear RNase P and RNase MRP ribonucleoproteins. RNase P consists of a catalytic RNA moiety and about 10 protein subunits; POP1, POP4, POP5, POP7, RPP14, RPP21, RPP25, RPP30, RPP38 and RPP40. Within the RNase P complex, POP1, POP7 and RPP25 form the 'finger' subcomplex, POP5, RPP14, RPP40 and homodimeric RPP30 form the 'palm' subcomplex, and RPP21, POP4 and RPP38 form the 'wrist' subcomplex. All subunits of the RNase P complex interact with the catalytic RNA. Several subunits of RNase P are also part of the RNase MRP complex. RNase MRP consists of a catalytic RNA moiety and about 8 protein subunits; POP1, POP7, RPP25, RPP30, RPP38, RPP40 and possibly also POP4 and POP5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic/archaeal RNase P protein component 3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002129371.1">
        <property type="nucleotide sequence ID" value="XM_002129335.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XK95"/>
    <dbReference type="FunCoup" id="H2XK95">
        <property type="interactions" value="781"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000030077"/>
    <dbReference type="Ensembl" id="ENSCINT00000035535.1">
        <property type="protein sequence ID" value="ENSCINP00000030077.1"/>
        <property type="gene ID" value="ENSCING00000019714.1"/>
    </dbReference>
    <dbReference type="GeneID" id="100177882"/>
    <dbReference type="KEGG" id="cin:100177882"/>
    <dbReference type="GeneTree" id="ENSGT00390000000883"/>
    <dbReference type="HOGENOM" id="CLU_048451_2_1_1"/>
    <dbReference type="InParanoid" id="H2XK95"/>
    <dbReference type="OMA" id="CYGPGIT"/>
    <dbReference type="OrthoDB" id="17948at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005655">
        <property type="term" value="C:nucleolar ribonuclease P complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000031">
        <property type="entry name" value="ribonuclease P protein subunit p30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002738">
        <property type="entry name" value="RNase_P_p30"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13031:SF0">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13031">
        <property type="entry name" value="RIBONUCLEASE P SUBUNIT P30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01876">
        <property type="entry name" value="RNase_P_p30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="PHP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="5" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00053284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00065838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00068480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00075070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000030077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="270" mass="30354" checksum="1FF33C021AD48BAF" modified="2012-04-18" version="1">MSSIPIPPHGKLYDLNIQYESNCLMETTSKANMAVRLGYEVVAINQNVSSVAKETKAKRMKLMLPEPPPLLHLDAESKSYLKINKKNFQQYSRITIELCELGDLHKVRDVLSTGLYDIIAVLPRTDRLFHSACTELHIDIICIDGSEKLPYLPKHATVQAAISRGINFEVCYAPMIRDTTMRRLTINNVQRLVESSKGKNLILSSGALHQMEMRGPYDVANLSTLFGIKENLHIHCVSNNCRSAIVHSFTRKTAKCAVYVQQLNETIATI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>H2XKL3</accession>
    <name>H2XKL3_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="H2XKL3"/>
    <dbReference type="Ensembl" id="ENSCINT00000036349.1">
        <property type="protein sequence ID" value="ENSCINP00000030195.1"/>
        <property type="gene ID" value="ENSCING00000024601.1"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3175141_0_0_1"/>
    <dbReference type="InParanoid" id="H2XKL3"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000030195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="47" mass="5389" checksum="D746FF01F8FD7281" modified="2012-04-18" version="1">MFIVFIKSNMLKIFQTYSPILSTARLMKVKCSCKVVSHGKCMLICFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>H2XLZ0</accession>
    <name>H2XLZ0_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <location>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
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                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
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                <person name="Zhang C."/>
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                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
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                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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            <authorList>
                <consortium name="Ensembl"/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
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                <person name="Doyle S."/>
                <person name="Francino P."/>
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                <person name="Hayashi H."/>
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                <person name="Imai K.S."/>
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                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
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                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
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                <person name="Miura S."/>
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                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
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                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
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                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
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                <person name="Harafuji N."/>
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                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
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                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">wild type</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="entry name" value="Tropomyosin"/>
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    <keyword evidence="2" id="KW-0472">Membrane</keyword>
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    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
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        <location>
            <begin position="15"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000032228.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    </evidence>
    <sequence length="115" mass="13678" checksum="897FB90D361DB59A" modified="2012-04-18" version="1">MEVMGPWIWAWVGPFYLYRQIFPLLPLISFFSNLNRKYKEVSSNLHLSLQCIKKYESMNEKLKEKEIQTEDELCTLQKRLYESENLRAIAENSLNNLAQQVGVLEDELYEVKMKV</sequence>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
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    <reference evidence="7" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">wild type</strain>
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    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the TRAFAC class dynamin-like GTPase superfamily. GB1/RHD3 GTPase family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="ENSCINP00000032288.1"/>
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    <dbReference type="GeneTree" id="ENSGT00940000165594"/>
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    <dbReference type="InParanoid" id="H2XRK4"/>
    <dbReference type="OMA" id="ACERNST"/>
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    </dbReference>
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        <source>
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        <source>
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</entry>
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            <fullName evidence="1">Uncharacterized protein</fullName>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
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                <person name="Doyle S."/>
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                <person name="Hayashi H."/>
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                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
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                <person name="Mochizuki Y."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
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                <person name="Di Gregorio A."/>
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                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
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                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
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                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
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            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1">wild type</strain>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
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                <person name="Di Gregorio A."/>
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                <person name="Goodstein D.M."/>
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                <person name="Hastings K.E."/>
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                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
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                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
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                <person name="Zeller R.W."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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                <consortium name="Ensembl"/>
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        <text evidence="5">Acts as component of the GARP complex that is involved in retrograde transport from early and late endosomes to the trans-Golgi network (TGN).</text>
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    <comment type="subunit">
        <text evidence="5">Component of the Golgi-associated retrograde protein (GARP) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Golgi apparatus</location>
            <location evidence="5">trans-Golgi network</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the VPS51 family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001768">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XTL8"/>
    <dbReference type="STRING" id="7719.ENSCINP00000033002"/>
    <dbReference type="Ensembl" id="ENSCINT00000031065.1">
        <property type="protein sequence ID" value="ENSCINP00000033002.1"/>
        <property type="gene ID" value="ENSCING00000018597.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000001738"/>
    <dbReference type="HOGENOM" id="CLU_071975_0_0_1"/>
    <dbReference type="InParanoid" id="H2XTL8"/>
    <dbReference type="OMA" id="MDEMATD"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
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        <property type="term" value="C:GARP complex"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="P:Golgi organization"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016159">
        <property type="entry name" value="Cullin_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039481">
        <property type="entry name" value="EXOC2/Sec5_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014812">
        <property type="entry name" value="Vps51"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15954">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 51 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15954:SF4">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 51 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15469">
        <property type="entry name" value="Sec5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74788">
        <property type="entry name" value="Cullin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exocyst complex component EXOC2/Sec5 N-terminal" evidence="7">
        <location>
            <begin position="38"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="97"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU368010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF15469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000033002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="335" mass="38374" checksum="BE1BAEF992006F03" modified="2012-04-18" version="1">MENDEDERRSRKKDMLKMYYGVNGADSNTAVGNDPLDIDSPHFHAENYLRKIKQEKSLAELMDAENDMAKQIRTLDSEMQTLVYENYNKFISATDTIRNMKRDFRQMENEMEKLTTNMSSITELNSEINETLKENRQKISKLSGVHSLLKKLQFLFELPAKLQKCLETGAFSTAVSYYMKTKSVLQHYQHMESFSGIERDCQAIINEIVAKLKQTFHDKHSSAQQLAECVGLLLRLGEPPGLLCDEYLQHSRHKLDEDLQELESYLVEEGVAIKSENSNSSHHLLIDVLEFIDRGSNGFLSNLSLTIAAYENLFIKQTEITSMTAALDGTYSPMM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>H2XU72</accession>
    <name>H2XU72_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="H2XU72"/>
    <dbReference type="Ensembl" id="ENSCINT00000035666.1">
        <property type="protein sequence ID" value="ENSCINP00000033206.1"/>
        <property type="gene ID" value="ENSCING00000023257.1"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2164025_0_0_1"/>
    <dbReference type="InParanoid" id="H2XU72"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003576998" evidence="4">
        <location>
            <begin position="21"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="84"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="91"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="62"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="74"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000033206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="111" mass="11902" checksum="F852400751AF240B" modified="2012-04-18" version="1">MFHVSAIVCIFLLCSNNVRGQELVNSQEGESRMDGTTQAPTTTTTEDYSYYQSCTGADEFDCGENICILRSKLCDGNFDCPDSNDEDGCPTTTAPNTTTAATTAQSTTTEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>H2XUS0</accession>
    <name>H2XUS0_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="H2XUS0"/>
    <dbReference type="Ensembl" id="ENSCINT00000036596.1">
        <property type="protein sequence ID" value="ENSCINP00000033404.1"/>
        <property type="gene ID" value="ENSCING00000023052.1"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2412578_0_0_1"/>
    <dbReference type="InParanoid" id="H2XUS0"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000033404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="92" mass="10711" checksum="C4D008F6B35A3E53" modified="2012-04-18" version="1">MNRTSKLWYDSIHLSDEGLSHMLKQIEGQLLNDQKVQVRIKSEFESNAARQTEKTSQTGKLRFPSLLQYSNIQFYIFLINSDCSNVCFCCKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>H2XV67</accession>
    <name>H2XV67_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>H2XVN4</accession>
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            <fullName evidence="11">General transcription factor IIH polypeptide 3</fullName>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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            <taxon>Eukaryota</taxon>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
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            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="12">wild type</strain>
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    <reference evidence="12" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="11">Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Part of a TFIID-containing RNA polymerase II pre-initiation complex that is composed of TBP and at least GTF2A1, GTF2A2, GTF2E1, GTF2E2, GTF2F1, GTF2H2, GTF2H3, GTF2H4, GTF2H5, GTF2B, TCEA1, ERCC2, ERCC3, TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13. Component of the 7-subunit TFIIH core complex composed of XPB/ERCC3, XPD/ERCC2, GTF2H1, GTF2H2, GTF2H3, GTF2H4 and GTF2H5, which is active in NER. The core complex associates with the 3-subunit CDK-activating kinase (CAK) module composed of CCNH/cyclin H, CDK7 and MNAT1 to form the 10-subunit holoenzyme (holo-TFIIH) active in transcription. Interacts with RARA; the interaction requires prior phosphorylation of RARA on 'Ser-369' which then enhances interaction of RARA with CDK7.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the TFB4 family.</text>
    </comment>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_002120767.5"/>
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        <property type="gene ID" value="ENSCING00000023995.1"/>
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    <keyword evidence="9 11" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3 11" id="KW-0479">Metal-binding</keyword>
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    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="7 11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6 11" id="KW-0862">Zinc</keyword>
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    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000033718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="309" mass="34442" checksum="7996A977E3701002" modified="2012-04-18" version="1">MANEEERQLLVVIFDVNPVWWGIKSLQGQAQITKCLDCLLVFVNSYLMLRHDNKLAIIASHSTKSVFLYPAQESSHSPDPSKASISDQAGGDCRYEHFAKVDDSVTDKFKELMRDGCEKNDIRRDSLIAGSMAIALCYIHRMQIESQGNTKLNARILVIKAADDAASQYMSFMNVIFAAQKERVVVDACILGQDSGLLQQACDMTSGMYLKVPHQDAFLQYLLTAFLTEAELRKESLVLAQSSRPREVDYRAACFCHRSLVDVGFVCSVCLSIFCQFSPICSTCETAFKPPVLASKSRKKRRALPANGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>H2XVP8</accession>
    <name>H2XVP8_CIOIN</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="H2XVP8"/>
    <dbReference type="Ensembl" id="ENSCINT00000032233.1">
        <property type="protein sequence ID" value="ENSCINP00000033732.1"/>
        <property type="gene ID" value="ENSCING00000023372.1"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3260256_0_0_1"/>
    <dbReference type="InParanoid" id="H2XVP8"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000033732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="42" mass="4480" checksum="E9FE7CBD0BD50E4A" modified="2012-04-18" version="1">MFSTKVVASVTSYGSDDHPLNKTSLSPLISIPTAILAFFILP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>H2XVS3</accession>
    <accession>A0A1W5BG60</accession>
    <name>H2XVS3_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Zinc finger protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">zf(c2h2)-17</name>
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    <organism evidence="14 15">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">wild type</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the requiem/DPF family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01000804">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="RefSeq" id="XP_018669421.1">
        <property type="nucleotide sequence ID" value="XM_018813876.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XVS3"/>
    <dbReference type="FunCoup" id="H2XVS3">
        <property type="interactions" value="326"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000033757"/>
    <dbReference type="Ensembl" id="ENSCINT00000033603.1">
        <property type="protein sequence ID" value="ENSCINP00000033757.1"/>
        <property type="gene ID" value="ENSCING00000018662.1"/>
    </dbReference>
    <dbReference type="GeneID" id="778805"/>
    <dbReference type="CTD" id="778805"/>
    <dbReference type="GeneTree" id="ENSGT00940000155070"/>
    <dbReference type="HOGENOM" id="CLU_038980_0_1_1"/>
    <dbReference type="InParanoid" id="H2XVS3"/>
    <dbReference type="OMA" id="NMIISVR"/>
    <dbReference type="OrthoDB" id="1903104at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 11"/>
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    <dbReference type="GO" id="GO:0071565">
        <property type="term" value="C:nBAF complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd15619">
        <property type="entry name" value="PHD1_d4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15530">
        <property type="entry name" value="PHD2_d4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000005">
        <property type="entry name" value="zinc finger protein isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="DPF1-3_N"/>
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    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45888:SF5">
        <property type="entry name" value="D4, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45888">
        <property type="entry name" value="HL01030P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14051">
        <property type="entry name" value="DPF1-3_N"/>
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    </dbReference>
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        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
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    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="186"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="12">
        <location>
            <begin position="277"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="12">
        <location>
            <begin position="334"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="133"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="216"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="142"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010539"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
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    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
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    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000033757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="399" mass="45459" checksum="FBEC6E9667F24FB5" modified="2012-04-18" version="1">MSGNDGFYKEALEHCRTFNSRLMTERRVRLPYLDSQTRVAQSDCCLWFKRRHRGPGLKPNQVYCYPSKRWKKRPRFTPLVDPSYEAMAAANDYKSMLRSSDVPHHLINENSNDSSSMNTITSAMIDADNEMLDNVSNNEDNGAIDEDEDFEDFENGKSKKKGRKSQFKAATRSGRKSDVTTDDKYHTCDYCGKKYKNKTGLNYHVQHVHQDELDADEEYTPSGRHSPSSIMSDDHHSTSSRTSAIAAQKAVAAAVADISEVEIIEPRPKRKRQTQSNNYCDFCLGDADENKKTGESEELVSCSDCGRSGHPTCLQFTDIMTMNVKKYSWQCIECKSCHVCGTSDNDEQLLFCDDCDRGYHMYCLQPRMENPPEGSWICNLCENDRKEREKLASHGTLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>H2XW20</accession>
    <name>H2XW20_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">D-galactoside-specific lectin-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC101242233</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="H2XW20"/>
    <dbReference type="Ensembl" id="ENSCINT00000032104.1">
        <property type="protein sequence ID" value="ENSCINP00000033854.1"/>
        <property type="gene ID" value="ENSCING00000018796.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154285"/>
    <dbReference type="HOGENOM" id="CLU_078349_1_0_1"/>
    <dbReference type="InParanoid" id="H2XW20"/>
    <dbReference type="OMA" id="NYGRTEN"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.740">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000922">
        <property type="entry name" value="Lectin_gal-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043159">
        <property type="entry name" value="Lectin_gal-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46780">
        <property type="entry name" value="PROTEIN EVA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02140">
        <property type="entry name" value="SUEL_Lectin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50228">
        <property type="entry name" value="SUEL_LECTIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SUEL-type lectin" evidence="1">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="SUEL-type lectin" evidence="1">
        <location>
            <begin position="71"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000033854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="163" mass="18295" checksum="117024361B87A75D" modified="2012-04-18" version="1">RPNTNIQSTSCASTSSNEIIKDACQGQNGCRLDARNSIFGDPCRGTPKYIYMSYTCIYPTCSNPIPTVRVICERQRRIINCGTGKRIGVLTGFYGRTNKLTFIAIRCPSRAIRSSRCKSRSSFRRIHNTCHGKQRCAITATNTVFGNPCNGTYKYIKMSYVCY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>H2XXA5</accession>
    <name>H2XXA5_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cadherin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">wild type</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="EAAA01002977">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XXA5"/>
    <dbReference type="Ensembl" id="ENSCINT00000032368.1">
        <property type="protein sequence ID" value="ENSCINP00000034289.1"/>
        <property type="gene ID" value="ENSCING00000022817.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165135"/>
    <dbReference type="HOGENOM" id="CLU_1113464_0_0_1"/>
    <dbReference type="InParanoid" id="H2XXA5"/>
    <dbReference type="OMA" id="ICISIID"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000020">
        <property type="entry name" value="Dachsous cadherin-related 1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000007">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050174">
        <property type="entry name" value="Protocadherin/Cadherin-CA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028:SF328">
        <property type="entry name" value="CADHERIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028">
        <property type="entry name" value="CADHERIN-87A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 10" id="KW-0106">Calcium</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Cadherin" evidence="11">
        <location>
            <begin position="2"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="11">
        <location>
            <begin position="107"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000034289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="250" mass="28340" checksum="78DD78910EEC5C60" modified="2012-04-18" version="1">MVNISESAAIHDVISLDRFLASDADIGNNSQLRYSISENDFFMLEQLVDETNTMHLQLKLLRKLDYERNSFQRLTLSARDRGVPIRSSHVQLRIRVTDDNDNDPIFEQLEYSVTLLENLPSGHVIARVQAHDADSGAAGRLRYSISRRNKATARNLVNINEINGDVVLRQKLDRESHNGLRVLIEARDSTPTNQRIGRTWLVIRVDDVNDNIPVISVNYILASNENTVYVKEDAMVGSYIAYVTAKDDDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>H2XY19</accession>
    <name>H2XY19_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">TNFR-Cys domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">wild type</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001764">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XY19"/>
    <dbReference type="STRING" id="7719.ENSCINP00000034553"/>
    <dbReference type="Ensembl" id="ENSCINT00000037056.1">
        <property type="protein sequence ID" value="ENSCINP00000034553.1"/>
        <property type="gene ID" value="ENSCING00000019827.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153259"/>
    <dbReference type="HOGENOM" id="CLU_1153885_0_0_1"/>
    <dbReference type="InParanoid" id="H2XY19"/>
    <dbReference type="OMA" id="SIFHIAY"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043123">
        <property type="term" value="P:positive regulation of canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046330">
        <property type="term" value="P:positive regulation of JNK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.10">
        <property type="entry name" value="Tumor Necrosis Factor Receptor, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001368">
        <property type="entry name" value="TNFR/NGFR_Cys_rich_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047526">
        <property type="entry name" value="TNR19/27/EDAR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12120">
        <property type="entry name" value="TNFR-CYS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12120:SF10">
        <property type="entry name" value="TNFR-CYS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00652">
        <property type="entry name" value="TNFR_NGFR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50050">
        <property type="entry name" value="TNFR_NGFR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="2 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003577907" description="TNFR-Cys domain-containing protein" evidence="12">
        <location>
            <begin position="19"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="145"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="repeat" description="TNFR-Cys" evidence="9">
        <location>
            <begin position="65"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="TNFR-Cys" evidence="13">
        <location>
            <begin position="65"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="209"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="209"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000034553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="241" mass="26811" checksum="CA2B951BE8060800" modified="2012-04-18" version="1">MNFSICVLLISIFHIAYGDEECVGEHEYVDKMGSCQQCEMCPPGYQPGKYHCGYGSKDIDYKCVACDSGTFSIATDYKLCVTCHPCRIWNRSTAQVCTSTSNARCGSCYTHFFRPLKSDGDFDDECHTCKPNSKKPECLNKENGWMSTGIIVIGVIAILFFILTLIVCVLYIRGRNNRATKPNEDAESNTTRIGSGLIRMLTGRCSHDTITSSSQTPLTTPDSQDIFAEKPRSQVNSPRMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>H2XY40</accession>
    <name>H2XY40_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01000229">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XY40"/>
    <dbReference type="Ensembl" id="ENSCINT00000034899.1">
        <property type="protein sequence ID" value="ENSCINP00000034574.1"/>
        <property type="gene ID" value="ENSCING00000019278.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000004915"/>
    <dbReference type="HOGENOM" id="CLU_2372113_0_0_1"/>
    <dbReference type="InParanoid" id="H2XY40"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040394">
        <property type="entry name" value="FBX25/32"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13123">
        <property type="entry name" value="LD30288P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13123:SF7">
        <property type="entry name" value="LD30288P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>H2Y1D6</accession>
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            <taxon>Eukaryota</taxon>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">wild type</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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        <property type="component" value="Chromosome 12"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000035720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="86" mass="9490" checksum="1D8D68C6CC08D6F0" modified="2012-04-18" version="1">FPLSAVLSPTGSCVLFKVFSFSALAVDFVSGSDNFSSFLTLKVRDNFRALELCFSLFVLSVDFFCASFDFFKDFKDKGGVLTSEVL</sequence>
</entry>
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    <accession>H2Y2U8</accession>
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        <recommendedName>
            <fullName evidence="10">Kinetochore protein NDC80</fullName>
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    </protein>
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        <name evidence="14" type="primary">LOC100185658</name>
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    <organism evidence="14 15">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Ciona</taxon>
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    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
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                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
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                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Putnam N."/>
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                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
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                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
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                <person name="Wiley G.B."/>
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                <person name="Roe B.A."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">wild type</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="10">Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the NDC80 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Chromosome</location>
            <location evidence="10">Centromere</location>
            <location evidence="10">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the NDC80/HEC1 family.</text>
    </comment>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_002120265.4"/>
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        <property type="interactions" value="123"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000036233"/>
    <dbReference type="Ensembl" id="ENSCINT00000030997.1">
        <property type="protein sequence ID" value="ENSCINP00000036233.1"/>
        <property type="gene ID" value="ENSCING00000024240.1"/>
    </dbReference>
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    <dbReference type="KEGG" id="cin:100185658"/>
    <dbReference type="GeneTree" id="ENSGT00390000018386"/>
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    <dbReference type="OMA" id="NKSWLMT"/>
    <dbReference type="OrthoDB" id="7459479at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031262">
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0051315">
        <property type="term" value="P:attachment of mitotic spindle microtubules to kinetochore"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Ndc80_CH"/>
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        <property type="entry name" value="Ndc80_sf"/>
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    <dbReference type="PANTHER" id="PTHR10643">
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        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR10643:SF2">
        <property type="entry name" value="KINETOCHORE PROTEIN NDC80 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ndc80_HEC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3 10" id="KW-0132">Cell division</keyword>
    <keyword evidence="9 10" id="KW-0137">Centromere</keyword>
    <keyword evidence="2 10" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 10" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="4 10" id="KW-0498">Mitosis</keyword>
    <keyword evidence="7 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="49"/>
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        <location>
            <begin position="428"/>
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        <location>
            <begin position="22"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00007050"/>
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        <source>
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        <source>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU368072"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF03801"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000036233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="596" mass="68567" checksum="E32BB45B60031E60" modified="2012-04-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
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    </protein>
    <organism evidence="6 7">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="H2Y2V4"/>
    <dbReference type="STRING" id="7719.ENSCINP00000036239"/>
    <dbReference type="Ensembl" id="ENSCINT00000034692.1">
        <property type="protein sequence ID" value="ENSCINP00000036239.1"/>
        <property type="gene ID" value="ENSCING00000023493.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000173714"/>
    <dbReference type="HOGENOM" id="CLU_000288_21_7_1"/>
    <dbReference type="InParanoid" id="H2Y2V4"/>
    <dbReference type="OMA" id="HEMHIVH"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051403">
        <property type="term" value="P:stress-activated MAPK cascade"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF288">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="13"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000036239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="209" mass="24078" checksum="2A237F1FEDBFE7FB" modified="2012-04-18" version="1">MKTFRNFQKSELSDVVSQVGFGGFGRVFHRKWRRGVQVIDVAEKVLIEHRIEERYARTILQEAEMLCMIKHQHVVKLFGVKHTDCSFSLIMEFVKHGSCTDFFKSVHLSREHLWKLKYRIALQVSGTMEYLHTMLPKRMLHLDLKSANVLLDATLNVKLCDFGLSQVHTLSRLATRRSVLSNRGDVTQHHVGGGTITHMAPEHLANPNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>H2Y2X4</accession>
    <name>H2Y2X4_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Gypsy retrotransposon integrase-like protein 1</fullName>
            <ecNumber evidence="2">3.1.26.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">zf(cchc)-26</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the beta type-B retroviral polymerase family. HERV class-II K(HML-2) pol subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.4" evidence="2"/>
    <dbReference type="STRING" id="7719.ENSCINP00000036259"/>
    <dbReference type="Ensembl" id="ENSCINT00000032173.1">
        <property type="protein sequence ID" value="ENSCINP00000036259.1"/>
        <property type="gene ID" value="ENSCING00000022489.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000169353"/>
    <dbReference type="HOGENOM" id="CLU_000384_42_12_1"/>
    <dbReference type="InParanoid" id="H2Y2X4"/>
    <dbReference type="OMA" id="ARECTAM"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00303">
        <property type="entry name" value="retropepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09274">
        <property type="entry name" value="RNase_HI_RT_Ty3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.10.10:FF:000007">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 17.6-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.370:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 17.6-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000032">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 297-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.70:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon gypsy-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.270:FF:000020">
        <property type="entry name" value="Transposon Tf2-6 polyprotein-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005162">
        <property type="entry name" value="Retrotrans_gag_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041373">
        <property type="entry name" value="RT_RNaseH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03732">
        <property type="entry name" value="Retrotrans_gag"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17917">
        <property type="entry name" value="RT_RNaseH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
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                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
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                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
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                <person name="Doggett N."/>
                <person name="Glavina T."/>
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                <person name="Richardson P."/>
                <person name="Lucas S."/>
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                <person name="Levine M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">wild type</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    </evidence>
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        <source>
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    </evidence>
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            <taxon>Ciona</taxon>
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    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2006" name="Peptides" volume="27" first="2535" last="2546">
            <title>Three insulin-relaxin-like genes in Ciona intestinalis.</title>
            <authorList>
                <person name="Olinski R.P."/>
                <person name="Dahlberg C."/>
                <person name="Thorndyke M."/>
                <person name="Hallbook F."/>
            </authorList>
            <dbReference type="PubMed" id="16920224"/>
            <dbReference type="DOI" id="10.1016/j.peptides.2006.06.008"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
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            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="7">wild type</strain>
        </source>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
            <location evidence="3">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the insulin family.</text>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="ABG00258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="gene ID" value="ENSCING00000003666.2"/>
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    <dbReference type="KEGG" id="cin:100170003"/>
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        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="P:cell population proliferation"/>
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        <property type="project" value="GO_Central"/>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="P:negative regulation of apoptotic process"/>
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    <feature type="compositionally biased region" description="Pro residues" evidence="4">
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        <source>
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        <source>
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    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000406"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="EMBL" id="ABG00258.1"/>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000007548.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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<entry dataset="TrEMBL" created="2005-08-30" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
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    <reference evidence="6" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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    <reference evidence="4" key="2">
        <citation type="journal article" date="2003" name="Dev. Genes Evol." volume="213" first="211" last="212">
            <title>Genomewide surveys of developmentally relevant genes in Ciona intestinalis.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Satoh N."/>
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            <dbReference type="DOI" id="10.1007/s00427-003-0330-z"/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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    <reference evidence="4" key="3">
        <citation type="journal article" date="2004" name="Development" volume="131" first="4047" last="4058">
            <title>Gene expression profiles of transcription factors and signaling molecules in the ascidian embryo: towards a comprehensive understanding of gene networks.</title>
            <authorList>
                <person name="Imai K.S."/>
                <person name="Hino K."/>
                <person name="Yagi K."/>
                <person name="Satoh N."/>
                <person name="Satou Y."/>
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            <dbReference type="DOI" id="10.1242/dev.01270"/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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    <reference evidence="4" key="4">
        <citation type="submission" date="2005-04" db="EMBL/GenBank/DDBJ databases">
            <title>Expressed genes in Ciona intestinalis.</title>
            <authorList>
                <person name="Satou Y."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="5" key="5">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">wild type</strain>
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    </reference>
    <reference evidence="5" key="6">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="1">Belongs to the PUR DNA-binding protein family.</text>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="EMBL" id="BAE06663.1"/>
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            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
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            <taxon>Ciona</taxon>
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    <reference evidence="15" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2003" name="Dev. Genes Evol." volume="213" first="211" last="212">
            <title>Genomewide surveys of developmentally relevant genes in Ciona intestinalis.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Satoh N."/>
            </authorList>
            <dbReference type="PubMed" id="12736827"/>
            <dbReference type="DOI" id="10.1007/s00427-003-0330-z"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="journal article" date="2004" name="Development" volume="131" first="4047" last="4058">
            <title>Gene expression profiles of transcription factors and signaling molecules in the ascidian embryo: towards a comprehensive understanding of gene networks.</title>
            <authorList>
                <person name="Imai K.S."/>
                <person name="Hino K."/>
                <person name="Yagi K."/>
                <person name="Satoh N."/>
                <person name="Satou Y."/>
            </authorList>
            <dbReference type="PubMed" id="15269171"/>
            <dbReference type="DOI" id="10.1242/dev.01270"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2005-04" db="EMBL/GenBank/DDBJ databases">
            <title>Expressed genes in Ciona intestinalis.</title>
            <authorList>
                <person name="Satou Y."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="14" key="5">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">wild type</strain>
        </source>
    </reference>
    <reference evidence="14" key="6">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the Antp homeobox family.</text>
    </comment>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="molecule type" value="mRNA"/>
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        <property type="gene ID" value="ENSCING00000006962.3"/>
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        <property type="entry name" value="HOMEOTIC PROTEIN SEX COMBS REDUCED"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00025">
        <property type="entry name" value="ANTENNAPEDIA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00032">
        <property type="entry name" value="ANTENNAPEDIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4 8 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 8 9" id="KW-0371">Homeobox</keyword>
    <keyword evidence="7 8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="12">
        <location>
            <begin position="107"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="8">
        <location>
            <begin position="109"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="51"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="170"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BAE06501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000014292.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="335" mass="38091" checksum="F853A80EB2574EF0" modified="2005-08-30" version="1">MSIIDQTELDFHNRGYVTNGIIKDRDVIDQGLPFFPTHHESAAALNLADMHGRGKEQAISSSKSSPEDDKTDEEYQAMGSRRSSNGPPVVYPWMKRIHVSQVINGLESGKRPRTAYTRHQVLELEKEFHYNRYLTRRRRIEIAHGLCLSERQVKIWFQNRRMKWKKDHKLPNTKVRNPPTSSLPILPTPSPPMTSLNHMLPNLSLPGSQSSPHNYDKSPPMTPTGGEYPIPNHALMHQPMTSRELNDAIGDSPTTEASYKSHDRRELDAMTSFQECNPPRNDHHPLMFDATGIPHSTFYGGVMSIPNSWNGDHSRAYISANQNSPYPSYPLSTFQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-07-10" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>Q4H3P5</accession>
    <name>DRI_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName>Protein dead ringer homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">Ci-DRIL1/2</name>
    </gene>
    <organism>
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Development" volume="131" first="4047" last="4058">
            <title>Gene expression profiles of transcription factors and signaling molecules in the ascidian embryo: towards a comprehensive understanding of gene networks.</title>
            <authorList>
                <person name="Imai K.S."/>
                <person name="Hino K."/>
                <person name="Yagi K."/>
                <person name="Satoh N."/>
                <person name="Satou Y."/>
            </authorList>
            <dbReference type="PubMed" id="15269171"/>
            <dbReference type="DOI" id="10.1242/dev.01270"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [MRNA]</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Transcription factor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AB210377">
        <property type="protein sequence ID" value="BAE06382.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001071682.1">
        <property type="nucleotide sequence ID" value="NM_001078214.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q4H3P5"/>
    <dbReference type="SMR" id="Q4H3P5"/>
    <dbReference type="FunCoup" id="Q4H3P5">
        <property type="interactions" value="5"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000036568"/>
    <dbReference type="Ensembl" id="ENSCINT00000037143.1">
        <property type="protein sequence ID" value="ENSCINP00000036568.1"/>
        <property type="gene ID" value="ENSCING00000020478.1"/>
    </dbReference>
    <dbReference type="GeneID" id="778579"/>
    <dbReference type="KEGG" id="cin:778579"/>
    <dbReference type="CTD" id="778579"/>
    <dbReference type="eggNOG" id="KOG2744">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000169348"/>
    <dbReference type="HOGENOM" id="CLU_026952_2_1_1"/>
    <dbReference type="InParanoid" id="Q4H3P5"/>
    <dbReference type="OMA" id="FECEREK"/>
    <dbReference type="OrthoDB" id="10044343at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd16867">
        <property type="entry name" value="ARID_ARID3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.60:FF:000007">
        <property type="entry name" value="AT-rich interactive domain-containing protein 3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.60">
        <property type="entry name" value="ARID DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045147">
        <property type="entry name" value="ARI3A/B/C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001606">
        <property type="entry name" value="ARID_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036431">
        <property type="entry name" value="ARID_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023334">
        <property type="entry name" value="REKLES_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15348">
        <property type="entry name" value="AT-RICH INTERACTIVE DOMAIN-CONTAINING PROTEIN ARID DOMAIN- CONTAINING PROTEIN DEAD RINGER PROTEIN B-CELL REGULATOR OF IGH TRANSCRIPTION BRIGHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15348:SF0">
        <property type="entry name" value="PROTEIN DEAD RINGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01388">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01014">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00501">
        <property type="entry name" value="BRIGHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46774">
        <property type="entry name" value="ARID-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51011">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51486">
        <property type="entry name" value="REKLES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0539">Nucleus</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <feature type="chain" id="PRO_0000295164" description="Protein dead ringer homolog">
        <location>
            <begin position="1"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="domain" description="ARID" evidence="2">
        <location>
            <begin position="249"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="REKLES" evidence="3">
        <location>
            <begin position="473"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="45"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="190"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="459"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="49"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="195"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="207"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="459"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="487"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="518"/>
            <end position="527"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="571" mass="63854" checksum="56E654247FE7E014" modified="2005-08-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-06-07" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1W5BJR6</accession>
    <accession>F6TBI8</accession>
    <name>A0A1W5BJR6_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Replication factor C subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100181422</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Subunit of the replication factor C (RFC) complex which acts during elongation of primed DNA templates by DNA polymerases delta and epsilon, and is necessary for ATP-dependent loading of proliferating cell nuclear antigen (PCNA) onto primed DNA. This subunit binds ATP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the activator 1 small subunits family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002130063.1">
        <property type="nucleotide sequence ID" value="XM_002130027.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018671143.1">
        <property type="nucleotide sequence ID" value="XM_018815598.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1W5BJR6"/>
    <dbReference type="FunCoup" id="A0A1W5BJR6">
        <property type="interactions" value="533"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000010059"/>
    <dbReference type="Ensembl" id="ENSCINT00000010059.3">
        <property type="protein sequence ID" value="ENSCINP00000010059.3"/>
        <property type="gene ID" value="ENSCING00000004870.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100181422"/>
    <dbReference type="KEGG" id="cin:100181422"/>
    <dbReference type="eggNOG" id="KOG0991">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00550000075050"/>
    <dbReference type="InParanoid" id="A0A1W5BJR6"/>
    <dbReference type="OMA" id="SCNYSSQ"/>
    <dbReference type="OrthoDB" id="4199794at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005663">
        <property type="term" value="C:DNA replication factor C complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18140">
        <property type="entry name" value="HLD_clamp_RFC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.272.10:FF:000006">
        <property type="entry name" value="Replication factor C subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000012">
        <property type="entry name" value="Replication factor C subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000107">
        <property type="entry name" value="Replication factor C subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.272.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008921">
        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050238">
        <property type="entry name" value="DNA_Rep/Repair_Clamp_Loader"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013748">
        <property type="entry name" value="Rep_factorC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047854">
        <property type="entry name" value="RFC_lid"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001679">
        <property type="entry name" value="PRK00440.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669">
        <property type="entry name" value="REPLICATION FACTOR C / DNA POLYMERASE III GAMMA-TAU SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669:SF5">
        <property type="entry name" value="REPLICATION FACTOR C SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08542">
        <property type="entry name" value="Rep_fac_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48019">
        <property type="entry name" value="post-AAA+ oligomerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="9">
        <location>
            <begin position="49"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00053246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000010059.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="336" mass="37593" checksum="76D0DE0B9E82C797" modified="2017-06-07" version="1">MEAMECTESSAKPAYELPWVEKYRPTKLSDVVGNEATVSRLEVFANEGNVPNIIIAGPPGAGKTTSIMCLARTMLGASYDVAVLELNASNDRGIDVVRNKIKMFAQKKVTLPKGKHKIIILDEADSMTSGAQQALRRTMEIYSKTTRFALACNQSDKIIEPIQSRCAVLRYSKLSDEQILSRLMEVIDKEGVRYDDSGLEALLFTAQGDMRQALNNLQSTHQGFSFINSDNVFKVCDEPHPLLLKTMLDHCVKADLDEAYKTINHLWAMGYSPDDIITNIFRVLKTHDIPEYVKLEFIKLVAETHMRIVQGVNSLLQLSALVARMCKICMKEQESA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>F6PIG9</accession>
    <accession>A0A1W2WH61</accession>
    <name>F6PIG9_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Vacuolar protein sorting-associated protein 28 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100182534</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the ESCRT-I complex (endosomal sorting complex required for transport I), a regulator of vesicular trafficking process.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the ESCRT-I complex (endosomal sorting complex required for transport I) which consists of TSG101, VPS28, a VPS37 protein (VPS37A to -D) and MVB12A or MVB12B in a 1:1:1:1 stoichiometry. Interacts with TSG101, VPS37B, VPS37C, MVB12A and MVB12B. Component of an ESCRT-I complex (endosomal sorting complex required for transport I) which consists of TSG101, VPS28, VPS37A and UBAP1 in a 1:1:1:1 stoichiometry. Interacts with VPS36; the interaction mediates the association with the ESCRT-II complex. Interacts with SNF8 and VPS25. Interacts with CEP55.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 7">Belongs to the VPS28 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002131096.1">
        <property type="nucleotide sequence ID" value="XM_002131060.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6PIG9"/>
    <dbReference type="FunCoup" id="F6PIG9">
        <property type="interactions" value="907"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000020236"/>
    <dbReference type="Ensembl" id="ENSCINT00000020236.3">
        <property type="protein sequence ID" value="ENSCINP00000020236.3"/>
        <property type="gene ID" value="ENSCING00000010116.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100182534"/>
    <dbReference type="KEGG" id="cin:100182534"/>
    <dbReference type="GeneTree" id="ENSGT00390000007486"/>
    <dbReference type="HOGENOM" id="CLU_076417_2_0_1"/>
    <dbReference type="InParanoid" id="F6PIG9"/>
    <dbReference type="OMA" id="CDEFPTV"/>
    <dbReference type="OrthoDB" id="2671at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000813">
        <property type="term" value="C:ESCRT I complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905603">
        <property type="term" value="P:regulation of blood-brain barrier permeability"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1130:FF:000001">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 28 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1440.200:FF:000001">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 28 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037202">
        <property type="entry name" value="ESCRT_assembly_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007143">
        <property type="entry name" value="Vps28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017899">
        <property type="entry name" value="VPS28_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037206">
        <property type="entry name" value="VPS28_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017898">
        <property type="entry name" value="VPS28_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038358">
        <property type="entry name" value="VPS28_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12937">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING 28, ISOFORM 2 VPS28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12937:SF0">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 28 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03997">
        <property type="entry name" value="VPS28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017535">
        <property type="entry name" value="VPS28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140111">
        <property type="entry name" value="Endosomal sorting complex assembly domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140427">
        <property type="entry name" value="VPS28 C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51310">
        <property type="entry name" value="VPS28_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51313">
        <property type="entry name" value="VPS28_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0967">Endosome</keyword>
    <keyword evidence="4 6 7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6 7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="VPS28 N-terminal" evidence="9">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="VPS28 C-terminal" evidence="8">
        <location>
            <begin position="112"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00062290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR017535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000020236.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="208" mass="23926" checksum="377DC04903DA44E0" modified="2011-07-27" version="1">MSTVRPELYQEVKLYSNAREREKYDNLAELYSIIKTLQALEKAYIKDSITAKDYTGACSKMLEQYKVAFRQVKTEVSTLDEFVKRYKLEDCHAALERIREDRPITIPDDGGNTSKCVADIVSLFITVMDKLRLDIRAMDELHPDLKDLSESMSRMTTLPMNFEGRTKVQAWLTTFAGMAASDDLTDPQARQMLFDMDSAYNAFNRFLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>F6PL48</accession>
    <name>F6PL48_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Kinesin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="F6PL48"/>
    <dbReference type="STRING" id="7719.ENSCINP00000006248"/>
    <dbReference type="Ensembl" id="ENSCINT00000006248.3">
        <property type="protein sequence ID" value="ENSCINP00000006248.3"/>
        <property type="gene ID" value="ENSCING00000003082.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000169691"/>
    <dbReference type="HOGENOM" id="CLU_001485_2_9_1"/>
    <dbReference type="InParanoid" id="F6PL48"/>
    <dbReference type="OMA" id="PENAWAG"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000245">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47117">
        <property type="entry name" value="STAR-RELATED LIPID TRANSFER PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0505">Motor protein</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="4">
        <location>
            <begin position="4"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="105"/>
            <end position="112"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000006248.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="221" mass="25108" checksum="258207E3A7DAB4CC" modified="2012-04-18" version="2">MSDNLKVAVRLRPFNEREKSRSSRLIINMNGGTTIISDPDDKYPDKKFTFDFSYWSHDGYRERDDGYLEPADQRYADQTKVFDDLGRGVLENAWKGFNCSLFAYGQTGSGKSYSMVGFDSNKGIIPITCEELFKGIAERKQTDEDEKEAEYQVSVSMLEIYNETVKDLLNISSFKKGGLKVREHPQKGFYVEQLQVSPVSDYAAIEGRIAEGTRNRTLAAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>F6PMS1</accession>
    <accession>A0A1W2WBH6</accession>
    <name>F6PMS1_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized LOC100181757</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC100181757</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01000153">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002128085.1">
        <property type="nucleotide sequence ID" value="XM_002128049.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6PMS1"/>
    <dbReference type="Ensembl" id="ENSCINT00000025320.2">
        <property type="protein sequence ID" value="ENSCINP00000025074.2"/>
        <property type="gene ID" value="ENSCING00000013717.2"/>
    </dbReference>
    <dbReference type="GeneID" id="100181757"/>
    <dbReference type="KEGG" id="cin:100181757"/>
    <dbReference type="HOGENOM" id="CLU_1065410_0_0_1"/>
    <dbReference type="InParanoid" id="F6PMS1"/>
    <dbReference type="OMA" id="MAACFWR"/>
    <dbReference type="OrthoDB" id="10571656at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030133">
        <property type="term" value="C:transport vesicle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028066">
        <property type="entry name" value="TMEM187"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15066">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 187"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15066:SF0">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 187"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15100">
        <property type="entry name" value="TMEM187"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="171"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="201"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="228"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000025074.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="260" mass="30284" checksum="6D92A0069D51C4FD" modified="2012-04-18" version="2">MAIPFDLQVLAPFSVLSITVIWTGIFDQSYTSSGSVLKELEGSTATNIWTFVCYFFMAACFWRVYEAWKYSNQVKITRVDVYFVSVLNGLALLQFAAHFLNYQSHFEPAKNVTTILRMSAMQDWSMLPFLAWNVSWSRHVRRGWTPWHNWMMCGLASISYFLTLIHRTSFFFLLGIAVTLSGIAVYETYKIHFYRAGIKPLFYVILSLHLIFYTASLLDFIILDFGWIFTYLSVTAFALMTALTNRLYIKILKEVNEHKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>F6PWH7</accession>
    <accession>A0A1W2WLP9</accession>
    <name>F6PWH7_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Methionyl-tRNA formyltransferase, mitochondrial</fullName>
            <ecNumber evidence="3">2.1.2.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100179457</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Methionyl-tRNA formyltransferase that formylates methionyl-tRNA in mitochondria and is crucial for translation initiation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-methionyl-tRNA(fMet) + (6R)-10-formyltetrahydrofolate = N-formyl-L-methionyl-tRNA(fMet) + (6S)-5,6,7,8-tetrahydrofolate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24380"/>
            <dbReference type="Rhea" id="RHEA-COMP:9952"/>
            <dbReference type="Rhea" id="RHEA-COMP:9953"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:78530"/>
            <dbReference type="ChEBI" id="CHEBI:78844"/>
            <dbReference type="ChEBI" id="CHEBI:195366"/>
            <dbReference type="EC" id="2.1.2.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:24381"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Fmt family.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.9" evidence="3"/>
    <dbReference type="RefSeq" id="XP_002129780.1">
        <property type="nucleotide sequence ID" value="XM_002129744.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6PWH7"/>
    <dbReference type="FunCoup" id="F6PWH7">
        <property type="interactions" value="31"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000001377"/>
    <dbReference type="Ensembl" id="ENSCINT00000001377.3">
        <property type="protein sequence ID" value="ENSCINP00000001377.3"/>
        <property type="gene ID" value="ENSCING00000000755.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100179457"/>
    <dbReference type="KEGG" id="cin:100179457"/>
    <dbReference type="GeneTree" id="ENSGT00390000017828"/>
    <dbReference type="HOGENOM" id="CLU_033347_0_0_1"/>
    <dbReference type="InParanoid" id="F6PWH7"/>
    <dbReference type="OMA" id="GASPIHE"/>
    <dbReference type="OrthoDB" id="10268103at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004479">
        <property type="term" value="F:methionyl-tRNA formyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071951">
        <property type="term" value="P:conversion of methionyl-tRNA to N-formyl-methionyl-tRNA"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd08646">
        <property type="entry name" value="FMT_core_Met-tRNA-FMT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12230:FF:000003">
        <property type="entry name" value="methionyl-tRNA formyltransferase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005793">
        <property type="entry name" value="Formyl_trans_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002376">
        <property type="entry name" value="Formyl_transf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036477">
        <property type="entry name" value="Formyl_transf_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041711">
        <property type="entry name" value="Met-tRNA-FMT_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11138">
        <property type="entry name" value="METHIONYL-TRNA FORMYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11138:SF5">
        <property type="entry name" value="METHIONYL-TRNA FORMYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02911">
        <property type="entry name" value="Formyl_trans_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00551">
        <property type="entry name" value="Formyl_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53328">
        <property type="entry name" value="Formyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Formyl transferase N-terminal" evidence="11">
        <location>
            <begin position="88"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="Formyl transferase C-terminal" evidence="12">
        <location>
            <begin position="213"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00052555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00057846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000001377.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="336" mass="38096" checksum="3A3F983BD68F5C08" modified="2012-04-18" version="2">MNFVKQFRKYTTIKQPPYKILFFGSDCFSLVTLKKLHNAYINGSLITKLEVMCLPHSKIRKSVVHEFATNHQIPIYYWGNFEFGGEFDLGVVVSFGKMMPQRIIDSFSLGMLNVHGSLLPQLRGSSPVSRAIQGGITSTGVSIFQIKSDGFDHGKILSYSSPVVIDDHVNSDELTQKLAYIGADLCLEVLWNFPERIANAKEQDRKLISYAPKINVQETFINWESMTPETILKNFRAFGYQKDYFLRCEYNGQMIKFLQLSSCTVEQTAFVSGNCPPGMVSYHKPSKTLLVKCCSGHVACTKLLYGKILSSADFYNGFWQKVKKQGKNVQFLSNKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>F6PZH8</accession>
    <name>F6PZH8_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Gap junction protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">wild type</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">A connexon is composed of a hexamer of connexins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Gap junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 9">Cell membrane</location>
            <topology evidence="2 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the connexin family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002036">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6PZH8"/>
    <dbReference type="STRING" id="7719.ENSCINP00000009550"/>
    <dbReference type="Ensembl" id="ENSCINT00000009550.3">
        <property type="protein sequence ID" value="ENSCINP00000009550.3"/>
        <property type="gene ID" value="ENSCING00000013396.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286949"/>
    <dbReference type="HOGENOM" id="CLU_037388_4_0_1"/>
    <dbReference type="InParanoid" id="F6PZH8"/>
    <dbReference type="OMA" id="FLLVMYP"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005922">
        <property type="term" value="C:connexin complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005243">
        <property type="term" value="F:gap junction channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007267">
        <property type="term" value="P:cell-cell signaling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.80">
        <property type="entry name" value="Gap junction channel protein cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000500">
        <property type="entry name" value="Connexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019570">
        <property type="entry name" value="Connexin_CCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017990">
        <property type="entry name" value="Connexin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013092">
        <property type="entry name" value="Connexin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038359">
        <property type="entry name" value="Connexin_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11984">
        <property type="entry name" value="CONNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11984:SF53">
        <property type="entry name" value="GAP JUNCTION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00029">
        <property type="entry name" value="Connexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00206">
        <property type="entry name" value="CONNEXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00037">
        <property type="entry name" value="CNX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01089">
        <property type="entry name" value="Connexin_CCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00408">
        <property type="entry name" value="CONNEXINS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0965">Cell junction</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0303">Gap junction</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="181"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="239"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="Connexin N-terminal" evidence="12">
        <location>
            <begin position="42"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="Connexin cysteine-rich" evidence="13">
        <location>
            <begin position="194"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="107"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="108"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000009550.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="336" mass="38504" checksum="54E42C1D69009A4D" modified="2012-04-18" version="2">MAWHIVEKLLEQVAEHSTLVGKFWVTFFFVLRFLMVVSIADTVFGDEQGSFVCNTLTPGCANICFNDFSPISLIRLWALQILSVSLPTIIFMVYTAHKMTKISQAKKLRQDEMDKKKKMREEAKKLKEEARKKAMADRRRRLEESGPPVKNEKKEKEPEKDKDDDKVVATKLDSDTPSRLMLAYVVQVVCRLAVEIGFMVLQFNIYVFKFWVPELFKCARWPCPNVVDCFVSRPKEKTIMLWVMFATGLIMIVLNLIELYHLGARKILEAWKQRGQDITKEYRVSSNMPHFGTHGGHGGGGRYPRGYPQAVGIRVGYARSSMSGEGGNDYDGDRFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>F6QPL4</accession>
    <name>F6QPL4_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Zinc finger protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">wild type</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="EAAA01002841">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6QPL4"/>
    <dbReference type="SMR" id="F6QPL4"/>
    <dbReference type="FunCoup" id="F6QPL4">
        <property type="interactions" value="77"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000013585"/>
    <dbReference type="Ensembl" id="ENSCINT00000013585.3">
        <property type="protein sequence ID" value="ENSCINP00000013585.3"/>
        <property type="gene ID" value="ENSCING00000006615.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000012556"/>
    <dbReference type="HOGENOM" id="CLU_084946_0_0_1"/>
    <dbReference type="InParanoid" id="F6QPL4"/>
    <dbReference type="OMA" id="FYRYSQF"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008988">
        <property type="term" value="F:rRNA (adenine-N6-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041370">
        <property type="entry name" value="Mlase_EEF1AKMT1/ZCCHC4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039846">
        <property type="entry name" value="ZCCHC4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017921">
        <property type="entry name" value="Znf_CTCHY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037275">
        <property type="entry name" value="Znf_CTCHY_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13493:SF3">
        <property type="entry name" value="RRNA N6-ADENOSINE-METHYLTRANSFERASE ZCCHC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13493">
        <property type="entry name" value="ZINC FINGER CCHC DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10237">
        <property type="entry name" value="N6-adenineMlase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161245">
        <property type="entry name" value="Zinc hairpin stack"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51270">
        <property type="entry name" value="ZF_CTCHY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CTCHY-type" evidence="7">
        <location>
            <begin position="169"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="6">
        <location>
            <begin position="240"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000013585.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="286" mass="33855" checksum="174AD45687CE8A93" modified="2012-04-18" version="2">MPRLHEYIQSTSNISSILLDIDNRYKMFWSNEQFCRFNMFNRHFFNPTSKLSFKKFLKSAEHPRNIVVVVDPPFGGLVDCVCESLKWISRFAFSLFGQNSTQLLTTLWLFPYFMEKRIKNQLQLTMLDYRVDYENHRKFKEAKHGRYMSPVRIFTNIKNKTVRLPSPDYSYRFCELCQRFVAKDNLHCKLCNDCPSKDGRTYRHCNICQICVKPGRKHCKTCDRCQPPLHSCNPHTSDGCHSCGEATHKRKDCPLLLNHQKTEVSLKKNKLLKFKSRKRKSFFSNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>F6QQY6</accession>
    <name>F6QQY6_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Helicase ATP-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">wild type</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01000610">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6QQY6"/>
    <dbReference type="STRING" id="7719.ENSCINP00000013247"/>
    <dbReference type="Ensembl" id="ENSCINT00000013247.3">
        <property type="protein sequence ID" value="ENSCINP00000013247.3"/>
        <property type="gene ID" value="ENSCING00000006431.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162718"/>
    <dbReference type="HOGENOM" id="CLU_092257_0_0_1"/>
    <dbReference type="InParanoid" id="F6QQY6"/>
    <dbReference type="OMA" id="WRRWIEN"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050628">
        <property type="entry name" value="SNF2_RAD54_helicase_TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626:SF50">
        <property type="entry name" value="TRANSCRIPTION TERMINATION FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626">
        <property type="entry name" value="TRANSCRIPTION TERMINATION FACTOR 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="4">
        <location>
            <begin position="1"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000013247.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="216" mass="25167" checksum="952A26C328133B89" modified="2025-10-08" version="3">MILAPASLIFHWKNEINNRCHKDLLSIHLYHGKDRERDAEKLAEFDVVITTYDVVRRTHPKPPKQTGLTTDTKPVTIDTKSDPLEHALFLIKWRRVILDEAHQIRNFKSQTSMAACALNAHSRWAMSGTPVQNQESDMYAMIKFLHCSPFDEHKLWKNQVSNNTTRGQQRLKTLVSCLVLRREKNQRGLDGKPLVKLPERKFEVHKLKLNDVEREV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>F6QZM4</accession>
    <accession>A0A1W2WMX2</accession>
    <name>F6QZM4_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Ubiquitin-conjugating enzyme E2 R2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100177981</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002130526.1">
        <property type="nucleotide sequence ID" value="XM_002130490.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6QZM4"/>
    <dbReference type="FunCoup" id="F6QZM4">
        <property type="interactions" value="303"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000016841"/>
    <dbReference type="Ensembl" id="ENSCINT00000016841.3">
        <property type="protein sequence ID" value="ENSCINP00000016841.3"/>
        <property type="gene ID" value="ENSCING00000008245.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100177981"/>
    <dbReference type="KEGG" id="cin:100177981"/>
    <dbReference type="GeneTree" id="ENSGT00940000167430"/>
    <dbReference type="HOGENOM" id="CLU_030988_1_2_1"/>
    <dbReference type="InParanoid" id="F6QZM4"/>
    <dbReference type="OMA" id="WNPTQNI"/>
    <dbReference type="OrthoDB" id="19692at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd23803">
        <property type="entry name" value="UBCc_UBE2R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000051">
        <property type="entry name" value="ubiquitin-conjugating enzyme E2 R2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="5">
        <location>
            <begin position="10"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="3">
        <location>
            <position position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000016841.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="230" mass="26294" checksum="82620CBE47A91357" modified="2011-07-27" version="1">MTNMPLPMASSAKALAKELMNLQEEPVEGFKITLVEENNLYDWQVAIFGPPGTLYQGGYFKAHIRFPGDYPYSPPSFRFLSHIWHPNVYENGEVCISILHAPIDDPQAGELPQERWNPTQNVRTILMSVISLLNEPNTFSPANVDASIMFRDWRERKSTKYEDYVKQQVRDSQREAERDGVVVPTTVEEYCVKTTVSHDSDSALNVEEFYDEDMEFCGDDDEDAPDVDTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>F6R5F9</accession>
    <name>F6R5F9_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Fibrocystin-L</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">wild type</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="EAAA01000516">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000016814"/>
    <dbReference type="Ensembl" id="ENSCINT00000016814.3">
        <property type="protein sequence ID" value="ENSCINP00000016814.3"/>
        <property type="gene ID" value="ENSCING00000008228.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157594"/>
    <dbReference type="HOGENOM" id="CLU_000057_1_0_1"/>
    <dbReference type="InParanoid" id="F6R5F9"/>
    <dbReference type="OMA" id="RSFPQKM"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00102">
        <property type="entry name" value="IPT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00603">
        <property type="entry name" value="IPT_PCSR"/>
        <property type="match status" value="9"/>
    </dbReference>
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        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="13"/>
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    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
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        <property type="entry name" value="CEMIP_beta-hel_dom"/>
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        <property type="entry name" value="Cupredoxin"/>
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        <property type="entry name" value="Fibrocystin"/>
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        <property type="entry name" value="G8_domain"/>
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        <property type="entry name" value="Ig-like_fold"/>
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        <property type="entry name" value="Ig_E-set"/>
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        <property type="entry name" value="IPT_dom"/>
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        <property type="entry name" value="PA14/GLEYA"/>
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        <property type="entry name" value="PbH1"/>
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    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
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    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
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        <property type="entry name" value="FIBROCYSTIN-L ISOFORM 2 PRECURSOR-RELATED"/>
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        <property type="entry name" value="POLYCYSTIC KIDNEY AND HEPATIC DISEASE 1 (AUTOSOMAL RECESSIVE)-LIKE 1"/>
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        <property type="entry name" value="CEMIP_beta-hel"/>
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    <dbReference type="Pfam" id="PF10162">
        <property type="entry name" value="G8"/>
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    <dbReference type="Pfam" id="PF01833">
        <property type="entry name" value="TIG"/>
        <property type="match status" value="12"/>
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    <dbReference type="SMART" id="SM01225">
        <property type="entry name" value="G8"/>
        <property type="match status" value="2"/>
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    <dbReference type="SMART" id="SM00429">
        <property type="entry name" value="IPT"/>
        <property type="match status" value="11"/>
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    <dbReference type="SMART" id="SM00710">
        <property type="entry name" value="PbH1"/>
        <property type="match status" value="8"/>
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    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="12"/>
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    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
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        <property type="match status" value="2"/>
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        <property type="entry name" value="PA14"/>
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    <proteinExistence type="predicted"/>
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    <keyword evidence="11" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
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    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
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        <location>
            <begin position="3018"/>
            <end position="3153"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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        <source>
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        <source>
            <dbReference type="PROSITE" id="PS51820"/>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000016814.3"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>F6RCK1</accession>
    <name>F6RCK1_CIOIN</name>
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        <recommendedName>
            <fullName evidence="16">SSD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">wild type</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>cholesterol(in) = cholesterol(out)</text>
            <dbReference type="Rhea" id="RHEA:39747"/>
            <dbReference type="ChEBI" id="CHEBI:16113"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the patched family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001866">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="F6RCK1">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000018303"/>
    <dbReference type="Ensembl" id="ENSCINT00000018303.3">
        <property type="protein sequence ID" value="ENSCINP00000018303.3"/>
        <property type="gene ID" value="ENSCING00000009011.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159904"/>
    <dbReference type="HOGENOM" id="CLU_002359_0_0_1"/>
    <dbReference type="InParanoid" id="F6RCK1"/>
    <dbReference type="OMA" id="QVFPYTI"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015485">
        <property type="term" value="F:cholesterol binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005319">
        <property type="term" value="F:lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042632">
        <property type="term" value="P:cholesterol homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008203">
        <property type="term" value="P:cholesterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030301">
        <property type="term" value="P:cholesterol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030299">
        <property type="term" value="P:intestinal cholesterol absorption"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015918">
        <property type="term" value="P:sterol transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1640.10">
        <property type="entry name" value="Multidrug efflux transporter AcrB transmembrane domain"/>
        <property type="match status" value="2"/>
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    <dbReference type="InterPro" id="IPR053958">
        <property type="entry name" value="HMGCR/SNAP/NPC1-like_SSD"/>
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    <dbReference type="InterPro" id="IPR004765">
        <property type="entry name" value="NPC1-like"/>
    </dbReference>
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        <property type="entry name" value="NPC1_MLD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032190">
        <property type="entry name" value="NPC1_N"/>
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    <dbReference type="InterPro" id="IPR000731">
        <property type="entry name" value="SSD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00917">
        <property type="entry name" value="2A060601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45727">
        <property type="entry name" value="NPC INTRACELLULAR CHOLESTEROL TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45727:SF3">
        <property type="entry name" value="NPC1-LIKE INTRACELLULAR CHOLESTEROL TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22314">
        <property type="entry name" value="NPC1_MLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16414">
        <property type="entry name" value="NPC1_N"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF12349">
        <property type="entry name" value="Sterol-sensing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82866">
        <property type="entry name" value="Multidrug efflux transporter AcrB transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50156">
        <property type="entry name" value="SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
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    <keyword evidence="6" id="KW-0732">Signal</keyword>
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    <keyword evidence="11" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="287"/>
            <end position="309"/>
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    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="358"/>
            <end position="380"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
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    <feature type="transmembrane region" description="Helical" evidence="15">
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    <feature type="transmembrane region" description="Helical" evidence="15">
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    <feature type="transmembrane region" description="Helical" evidence="15">
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    <feature type="transmembrane region" description="Helical" evidence="15">
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    <feature type="transmembrane region" description="Helical" evidence="15">
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    <feature type="transmembrane region" description="Helical" evidence="15">
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    <feature type="transmembrane region" description="Helical" evidence="15">
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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        <source>
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        <source>
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            <fullName evidence="1">Fibrinogen C-terminal domain-containing protein</fullName>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
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    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <consortium name="Ensembl"/>
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            <authorList>
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    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000028183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="197" mass="21651" checksum="BF76E2ADCAAE6AD8" modified="2011-07-27" version="1">MTQPLELGVEVRDVGRHLTGDTRTMKTMVTIKVEDGDDQPPAFEPCTKVAGKCVNPVYKTTINRFARSNKVLVLEPAQLYAVDPDLQIRQTIKYSLLAVNPEGLESYFTVEPSTGGVRLRRSLPETGIPEDIEVFVQASQSDNERMSAIATVQIHVQGRMSVSTKPEILTTNLTQVQAVVNSIDDLSLVRTLAGNIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F6S750</accession>
    <name>F6S750_CIOIN</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01001285">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6S750"/>
    <dbReference type="Ensembl" id="ENSCINT00000014992.3">
        <property type="protein sequence ID" value="ENSCINP00000014992.3"/>
        <property type="gene ID" value="ENSCING00000007308.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000068144"/>
    <dbReference type="HOGENOM" id="CLU_1242548_0_0_1"/>
    <dbReference type="InParanoid" id="F6S750"/>
    <dbReference type="OMA" id="NCEAITE"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="58"/>
            <end position="82"/>
        </location>
    </feature>
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        <location>
            <begin position="146"/>
            <end position="223"/>
        </location>
    </feature>
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        <location>
            <begin position="7"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="188"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000014992.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="223" mass="24168" checksum="4B4861AD8AFADAA1" modified="2012-04-18" version="2">MTDDDIDMGHNVHGKDKQAAPDSVLGKGCELKRHNDNCEAITELNASSQVRPIESRVVASHSDVTSTSINGNGPISKQESSGITEACLNTSNAEKSVEQIVVEPSFVSFEGQKVYKASTPYRSPVTSTISSQECSSFRNKVTIVHKKPDFPSTSPEPQGKMKLKKEVIAKRQASSPRVKTPDVKDAKSYTSTVTLSLKPSAKVRTPTENKEDLSFYTNPGANP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>F6S8Y1</accession>
    <accession>A0A1W2WH96</accession>
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        <recommendedName>
            <fullName evidence="3">Glyoxylate reductase/hydroxypyruvate reductase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100184381</name>
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    <organism evidence="7 8">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
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    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">wild type</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_002131111.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6S8Y1"/>
    <dbReference type="STRING" id="7719.ENSCINP00000002690"/>
    <dbReference type="Ensembl" id="ENSCINT00000002690.3">
        <property type="protein sequence ID" value="ENSCINP00000002690.3"/>
        <property type="gene ID" value="ENSCING00000001383.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100184381"/>
    <dbReference type="KEGG" id="cin:100184381"/>
    <dbReference type="GeneTree" id="ENSGT00940000162740"/>
    <dbReference type="HOGENOM" id="CLU_019796_1_2_1"/>
    <dbReference type="InParanoid" id="F6S8Y1"/>
    <dbReference type="OMA" id="MNVLYYN"/>
    <dbReference type="OrthoDB" id="298012at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="F:glyoxylate reductase (NADPH) activity"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="F:hydroxypyruvate reductase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Glyoxylate/hydroxypyruvate reductase B"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00073306"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003719"/>
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    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00389"/>
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    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
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    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000002690.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="328" mass="36283" checksum="11FA1ABA88D9CA2C" modified="2011-07-27" version="1">MSKPLVLCSYLDRSAGRWNGVTDFFVNIFEKRFKIIWMDDFEQDVAKYAKDVVAIASFIKRPPVTEELIKKLPNLKIVANIGVGYNHLDVPMIRSFGVKVSNTPLVLDDATSDLGMALLLNAGRQLHSNITLLRSPETTQIDTNYMTNDVSGTTIGILGMGRIGYKVAQRAKAFNMKILYHNRSRRDEGEEANIGAQYYSNLNEMLPHCDYVMVTLPLTAETQKIMGSAQFKLMKSTAILVNISRGGTVDQDALVHALTHGEIQFAALDVTEPEPLPRDHKLLSLENVIITPHMGSGTLNTRIAMAQKAFDNVVAAVDGTALPSEVFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>F6S9V9</accession>
    <name>F6S9V9_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">wild type</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01000826">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6S9V9"/>
    <dbReference type="STRING" id="7719.ENSCINP00000002671"/>
    <dbReference type="Ensembl" id="ENSCINT00000002671.3">
        <property type="protein sequence ID" value="ENSCINP00000002671.3"/>
        <property type="gene ID" value="ENSCING00000001370.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162823"/>
    <dbReference type="HOGENOM" id="CLU_549743_0_0_1"/>
    <dbReference type="InParanoid" id="F6S9V9"/>
    <dbReference type="OMA" id="ISAYNVP"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000003">
        <property type="entry name" value="Transmembrane serine protease 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252">
        <property type="entry name" value="ACROSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252:SF7">
        <property type="entry name" value="HYALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 6" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="CUB" evidence="7">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="7">
        <location>
            <begin position="99"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="8">
        <location>
            <begin position="241"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="153"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000002671.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="496" mass="54834" checksum="60A5C60952F12740" modified="2012-04-18" version="2">MIRRSQRDIEAGNKLLLRVVSLQMETLPNCVNDYLRIFTTTYNDVTQTFENRRLVRQICSHQGGDIVLSQDSLLIVFHSDSSIESSGFHLDLRSISDSCGGSVLTDGTIIFSPNYPNRYSPGLQCIWTITAPSSYYYVEVEFTKLQLNDSEGCRLDYLAIQAGNTSQRLCGIKTNSPRLQFNQSNLTLTFRTRLRRNNPTTGKGFSATVRFLPRYGRARPSECGVQSILPFGVGGPNTDRIVGGSEAIPGSWPWLVRIREANCGGALLSDRWILTAAHCLVGTAPRSIRTYDVNATIFSALMGRHDIQLPSINEQILRISKVVIHPEYRRFYPSRSRPFAFGVSGVPLNDIALIKLSTPVVFTPAIHPVCLPNQHVSVGYLCVVAGWGSRGSQRFSRRTPLHFAQVPILDNDICSSWPALHGAVTSTNLCAGYPGGGVDSCWGDSGGPLMCRGGDGVYYLHGVVSWGKGCGKPRQPGMYTKVRSYIDWIRRTTTND</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>F6SE21</accession>
    <name>F6SE21_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">cGMP-dependent protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.12</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.12"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cGMP subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.12" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="F6SE21"/>
    <dbReference type="FunCoup" id="F6SE21">
        <property type="interactions" value="6"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000000752"/>
    <dbReference type="Ensembl" id="ENSCINT00000000752.3">
        <property type="protein sequence ID" value="ENSCINP00000000752.3"/>
        <property type="gene ID" value="ENSCING00000000405.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166710"/>
    <dbReference type="HOGENOM" id="CLU_000288_73_2_1"/>
    <dbReference type="InParanoid" id="F6SE21"/>
    <dbReference type="OMA" id="YDENREF"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004691">
        <property type="term" value="F:cAMP-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030553">
        <property type="term" value="F:cGMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004692">
        <property type="term" value="F:cGMP-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000210">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002374">
        <property type="entry name" value="cGMP_dep_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353:SF37">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE CATALYTIC SUBUNIT PRKX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-DEPENDENT PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000559">
        <property type="entry name" value="cGMP-dep_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0140">cGMP</keyword>
    <keyword evidence="11" id="KW-0142">cGMP-binding</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="18">
        <location>
            <begin position="18"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="18">
        <location>
            <begin position="144"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="293"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="19">
        <location>
            <begin position="561"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="15">
        <location>
            <position position="419"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="323"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR000559-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR000559-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000000752.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="611" mass="69448" checksum="11E41A1909F8B924" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>F6SIZ4</accession>
    <name>F6SIZ4_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Poly [ADP-ribose] polymerase</fullName>
            <shortName evidence="7">PARP</shortName>
            <ecNumber evidence="7">2.4.2.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">wild type</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ARTD/PARP family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.-" evidence="7"/>
    <dbReference type="EMBL" id="EAAA01001957">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="EAAA01001958">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6SIZ4"/>
    <dbReference type="Ensembl" id="ENSCINT00000003792.3">
        <property type="protein sequence ID" value="ENSCINP00000003792.3"/>
        <property type="gene ID" value="ENSCING00000001882.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164121"/>
    <dbReference type="HOGENOM" id="CLU_012160_2_0_1"/>
    <dbReference type="InParanoid" id="F6SIZ4"/>
    <dbReference type="OMA" id="SHENCNS"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003950">
        <property type="term" value="F:NAD+ poly-ADP-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010629">
        <property type="term" value="P:negative regulation of gene expression"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.228.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.220.10">
        <property type="entry name" value="Leucine Aminopeptidase, subunit E, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002589">
        <property type="entry name" value="Macro_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043472">
        <property type="entry name" value="Macro_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052056">
        <property type="entry name" value="Mono-ARTD/PARP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012317">
        <property type="entry name" value="Poly(ADP-ribose)pol_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14453">
        <property type="entry name" value="PARP/ZINC FINGER CCCH TYPE DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14453:SF67">
        <property type="entry name" value="POLY [ADP-RIBOSE] POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01661">
        <property type="entry name" value="Macro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00644">
        <property type="entry name" value="PARP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00506">
        <property type="entry name" value="A1pp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56399">
        <property type="entry name" value="ADP-ribosylation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52949">
        <property type="entry name" value="Macro domain-like"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS51154">
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            <end position="226"/>
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    <feature type="domain" description="PARP catalytic" evidence="8">
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        <source>
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            <dbReference type="PROSITE" id="PS51154"/>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    </evidence>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
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                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
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                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
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                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
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                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
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    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000026923.2"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="202" mass="23241" checksum="9CD596A11AF57C53" modified="2012-04-18" version="2">MTDKGNHVFYLNDSSGPQTANRRLGSWMKVDMKGVKVKCNNDTNIKNKTMIIQVNFAHVMLEKCGNIYNRRGITYMRNTGLYWKRFNKIALLQDARISNVEKKWDIFNKLNLTSTQHNSTQITDFCIVEGRLFVVNEIGIVSERLGISDAFPDGTGWTELNNAEIDAKPQQIWISCFSCHWRQKTLVWTIGYGGTLTRLIIE</sequence>
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        <name type="scientific">Ciona intestinalis</name>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
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                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MTFR1 family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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        <location>
            <begin position="142"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="142"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="164"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    </evidence>
    <sequence length="181" mass="19777" checksum="881632371814E5E1" modified="2012-04-18" version="2">MNEGKENQNTNKMDDVRAGNRRSLVRMIGAHLPLSITPRPRFEILPNGARLIDDDQRKTLFSLSDICQLIDIPDVFGRSQSCEDLKEISKQPDTTIMTSSSMTSLPPDTMETKITALEDELSMLRSQIAMLVSNQTKQQTDLSTSFNSTISCAGEPASSSTPIKAPPPPGIPPPCMPPPPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
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    <name>F6SXP9_CIOIN</name>
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        <submittedName>
            <fullName evidence="14">Netrin receptor UNC5B-a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC100176438</name>
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    <organism evidence="14 15">
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        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
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            <taxon>Ciona</taxon>
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    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="14" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="14">wild type</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the immunoglobulin superfamily. DCC family.</text>
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    <comment type="similarity">
        <text evidence="3">Belongs to the unc-5 family.</text>
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        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
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        <property type="entry name" value="Ig_sub2"/>
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        <property type="entry name" value="SEMA DOMAIN-CONTAINING PROTEIN"/>
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        <property type="entry name" value="TSP_1"/>
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        <property type="entry name" value="Unc5_NetrinR_N"/>
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        <property type="entry name" value="IGc2"/>
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        <source>
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        <source>
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    <evidence type="ECO:0000256" key="4">
        <source>
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        <source>
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        <source>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
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        <recommendedName>
            <fullName evidence="18">Histone-lysine N-methyltransferase eggless</fullName>
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    <organism evidence="16 17">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
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            <taxon>Ciona</taxon>
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    <reference evidence="17" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="PubMed" id="18854010"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">wild type</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="16" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:histone H3K9 methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:heterochromatin organization"/>
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        <property type="project" value="GO_Central"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0010629">
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    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
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        <location>
            <begin position="290"/>
            <end position="364"/>
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            <begin position="916"/>
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        <location>
            <begin position="569"/>
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            <begin position="572"/>
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        <location>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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            <dbReference type="ARBA" id="ARBA00022833"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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            <dbReference type="PROSITE" id="PS50280"/>
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            <dbReference type="PROSITE" id="PS50867"/>
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            <dbReference type="PROSITE" id="PS50868"/>
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        <source>
            <dbReference type="PROSITE" id="PS50982"/>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000019485.3"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    </evidence>
    <sequence length="932" mass="106394" checksum="DE4E28EECEA7EEC4" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>F6T949</accession>
    <name>F6T949_CIOIN</name>
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        <recommendedName>
            <fullName evidence="7">Peptidase M41 domain-containing protein</fullName>
        </recommendedName>
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    <organism evidence="8 9">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
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            <taxon>Phlebobranchia</taxon>
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            <taxon>Ciona</taxon>
        </lineage>
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    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">wild type</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="EAAA01000343">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6T949"/>
    <dbReference type="STRING" id="7719.ENSCINP00000015464"/>
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        <property type="protein sequence ID" value="ENSCINP00000015464.3"/>
        <property type="gene ID" value="ENSCING00000007547.3"/>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="InterPro"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:metalloendopeptidase activity"/>
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        <property type="project" value="InterPro"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="ATP-dependent zinc metalloprotease FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Peptidase M41"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Peptidase_M41-like"/>
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        <property type="entry name" value="ATP-DEPENDENT ZINC METALLOPROTEASE YME1L1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="METALLOPROTEASE M41 FTSH"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="FtsH protease domain-like"/>
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    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M41" evidence="7">
        <location>
            <begin position="48"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF01434"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000015464.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="264" mass="29558" checksum="21100B4F96601CF3" modified="2012-04-18" version="2">MTGADISNIVNQAALRAAKLNKQRVDQDDLEYALDKAKMGPELKSRIRSESELRNTAHHEAGHALVAYYTPAAHTIYKATIRQRGPALGHVSLIPEKESEQSLSKAQIISMMDVSMGGRVAEELLLGKEHVSTGASSDINNATESAYKLVCMYGMSEKLGLMTYDMQHLSQETKRAVEIEVKKLLEKSYEKAKEMIVSKSYEHKLLAEALLRYETLTMDEIKILLDSKDINSIENMRLHTQQQKILQKSELEKLKDALAPGLLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>F6U981</accession>
    <name>F6U981_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CCR4-NOT transcription complex subunit 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Kohara Y."/>
                <person name="Levine M."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="F6URE2"/>
    <dbReference type="Ensembl" id="ENSCINT00000004698.3">
        <property type="protein sequence ID" value="ENSCINP00000004698.3"/>
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    <dbReference type="OMA" id="SWHASKH"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
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    <proteinExistence type="predicted"/>
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        <location>
            <begin position="60"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="213"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="274"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000004698.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="289" mass="32600" checksum="4FC802CDA8F6AD63" modified="2012-04-18" version="2">MRDEAIQLVKASQQNLVMEIERGIPPAHPSYPLRHERHGYYITKPSYTYQEAPRRTIENEYDSSRDELVTSQSSLHRFDDDYTTPDVATTQAPYPPSGRNERLEQPSTSDVTQGPPVKLRARKARDRGVPTERPRSWHASKHPDDTTYMHDDRKNMRSPGYPGSPASQPRFHLLSQPQRRSKGWYESGDLKSAIRGDVNGSKDSLISEHSQHSYRSGRLSVSSSRSSGTMSSSRGSLDNLDKPHRADIGSNRATSKRKSPSKPVAGIVTQQKNVFEKLSHHPPYEEGGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>F6V276</accession>
    <name>F6V276_CIOIN</name>
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        <recommendedName>
            <fullName evidence="7">Tetraspanin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">wild type</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the tetraspanin (TM4SF) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01000454">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="EAAA01000455">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6V276"/>
    <dbReference type="Ensembl" id="ENSCINT00000012935.3">
        <property type="protein sequence ID" value="ENSCINP00000012935.3"/>
        <property type="gene ID" value="ENSCING00000006267.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165843"/>
    <dbReference type="HOGENOM" id="CLU_055524_10_1_1"/>
    <dbReference type="InParanoid" id="F6V276"/>
    <dbReference type="OMA" id="YSKSDAY"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03127">
        <property type="entry name" value="tetraspanin_LEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1450.10">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000301">
        <property type="entry name" value="Tetraspanin_animals"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008952">
        <property type="entry name" value="Tetraspanin_EC2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282:SF551">
        <property type="entry name" value="RE08073P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002419">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00259">
        <property type="entry name" value="TMFOUR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48652">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="148"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="83"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="84"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR002419-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000012935.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="183" mass="19312" checksum="9F9A9B4CF0DBA2AB" modified="2012-04-18" version="2">MFVVGFFGCCGAIKENKCLLGTYFTCLLIIFLAQVGIGIWALVSQSSIETEIKKGLNSTLPLDYSKSDAYANTVVAVQQSFKCCGLVTGCTDWIGGNTTGCSCTPVITNSTVCAVPSSGTCINGDTPGGAIYTSDCYDAIIKFINDNIYLIAGIGLGIGLAEIFGMIFALVMCRSEKSGYETY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>F6V771</accession>
    <name>F6V771_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">CAAX prenyl protease</fullName>
            <ecNumber evidence="9">3.4.24.84</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100176674</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">wild type</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Proteolytically removes the C-terminal three residues of farnesylated proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 9">
            <text>Hydrolyzes the peptide bond -P2-(S-farnesyl or geranylgeranyl)C-P1'-P2'-P3'-COOH where P1' and P2' are amino acids with aliphatic side chains and P3' is any C-terminal residue.</text>
            <dbReference type="EC" id="3.4.24.84"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8 9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8 9">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Endoplasmic reticulum membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the peptidase M48A family.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.84" evidence="9"/>
    <dbReference type="EMBL" id="EAAA01002308">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6V771"/>
    <dbReference type="FunCoup" id="F6V771">
        <property type="interactions" value="398"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000012053"/>
    <dbReference type="MEROPS" id="M48.003"/>
    <dbReference type="Ensembl" id="ENSCINT00000012053.3">
        <property type="protein sequence ID" value="ENSCINP00000012053.3"/>
        <property type="gene ID" value="ENSCING00000005845.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000002053"/>
    <dbReference type="HOGENOM" id="CLU_025947_2_0_1"/>
    <dbReference type="InParanoid" id="F6V771"/>
    <dbReference type="OMA" id="HITHRTL"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071586">
        <property type="term" value="P:CAAX-box protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07343">
        <property type="entry name" value="M48A_Zmpste24p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2010.10">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027057">
        <property type="entry name" value="CAXX_Prtase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001915">
        <property type="entry name" value="Peptidase_M48"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10120">
        <property type="entry name" value="CAAX PRENYL PROTEASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01435">
        <property type="entry name" value="Peptidase_M48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 9" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1 9" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8 9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M48" evidence="11">
        <location>
            <begin position="21"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="82"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="97"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="137"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="220"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="216"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR627057-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR627057-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU366005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000012053.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="278" mass="32217" checksum="1B01441661F4F351" modified="2012-04-18" version="2">MVLLHIYPEFIAPLFDKYNPLPDGELKTKINELAQSLDFPLQKLYVVEGSKRSAHSNAYMYGFHKNKRIVLFDTLLEDYTPVGRTDQTNSDSTEVPESDREESNSGKGEDVNKTDKDCETKKIGCNTEEVVAVLGHELGHWKFSHMLKNIVIMQGSILFSFFVFGQLMNNKQIFAAFGFIDQQPILIRLYIVFSFIFSPINEIESFLMHILSRRFEFQADEFAVNLGKREKLKSALLKLFKDNLAFPVADWMYSARHYSHPPLLERLRAMDQYKPKQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>F6W2L5</accession>
    <accession>A0A1W2W8V4</accession>
    <name>F6W2L5_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Fumarylacetoacetate hydrolase domain-containing protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100181820</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">wild type</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAH family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002200">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="EAAA01002201">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_026690421.1">
        <property type="nucleotide sequence ID" value="XM_026834620.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6W2L5"/>
    <dbReference type="FunCoup" id="F6W2L5">
        <property type="interactions" value="5"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000016606"/>
    <dbReference type="Ensembl" id="ENSCINT00000016606.3">
        <property type="protein sequence ID" value="ENSCINP00000016606.3"/>
        <property type="gene ID" value="ENSCING00000012146.2"/>
    </dbReference>
    <dbReference type="GeneID" id="100181820"/>
    <dbReference type="KEGG" id="cin:100181820"/>
    <dbReference type="GeneTree" id="ENSGT00940000166311"/>
    <dbReference type="HOGENOM" id="CLU_028458_3_2_1"/>
    <dbReference type="InParanoid" id="F6W2L5"/>
    <dbReference type="OMA" id="CNKIVAP"/>
    <dbReference type="OrthoDB" id="411064at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050163">
        <property type="term" value="F:oxaloacetate tautomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006107">
        <property type="term" value="P:oxaloacetate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.850.10:FF:000002">
        <property type="entry name" value="2-hydroxyhepta-2,4-diene-1,7-dioate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.850.10">
        <property type="entry name" value="Fumarylacetoacetase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051121">
        <property type="entry name" value="FAH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011234">
        <property type="entry name" value="Fumarylacetoacetase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036663">
        <property type="entry name" value="Fumarylacetoacetase_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42796:SF4">
        <property type="entry name" value="FUMARYLACETOACETATE HYDROLASE DOMAIN-CONTAINING PROTEIN 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42796">
        <property type="entry name" value="FUMARYLACETOACETATE HYDROLASE DOMAIN-CONTAINING PROTEIN 2A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01557">
        <property type="entry name" value="FAA_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56529">
        <property type="entry name" value="FAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fumarylacetoacetase-like C-terminal" evidence="3">
        <location>
            <begin position="106"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000016606.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="315" mass="34854" checksum="96CEBE112DD2A77E" modified="2012-04-18" version="2">MLPLHLLRPLIAVTLHTRAFSLSLQRNMRLLQFLQDGKQSVGAELQDTSIVNLSSSNPQIPNSLVKFLESGESNFNLAKKALESSTKQFVQRSDIKILSPITNCEKVICVGMNYKDHCEEQNMPIPTEPVIFSKFPSSIIGDGDDIIIPKISDSVDWEVELAVIIGKEGKHIKESNALDYVAGYTVAHDVSARDWQMKRNSKQWLLGKTFDTFCPLGPVMVTKDEIKDPHNLSLECLVNGEVMQSSNTNQLVFSIETCIAWISRVFTLRPGDVLLTGTPPGVGVFRNPPVFLKAGDTVVCDIEGIGKITNKMVAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F6WN88</accession>
    <accession>A0A1W3JQM2</accession>
    <name>F6WN88_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Myosin heavy chain, striated muscle-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC100183561</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="136"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000010202.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="249" mass="27657" checksum="FD9854CEA178EEF4" modified="2012-04-18" version="2">MQDLQTDTSTCQWGSLGRLSKLKTLSLPINSYLPPETAVSGIDACLQTDLRGGTTSSNNCPTISNSSFNGDSTITTGDVRSVNALKDEQAKCKFFGGGADECTNTAHPRHNHGESSSSGYVTQDTLLSSPGTKGARNSDRSRRDNSSVEDNYEWDSEYAMESEILEALKNFESLKSSTSVSEELRRQLCKFTVNAESDDSSMGVDATYEAIKNDSEMREEDLKKEDIERRCAILKREFLHFRRLQQDEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>F6XPI8</accession>
    <name>F6XPI8_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">palmitoyl-protein hydrolase</fullName>
            <ecNumber evidence="2">3.1.2.22</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">wild type</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily. AB hydrolase 2 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.22" evidence="2"/>
    <dbReference type="EMBL" id="EAAA01000054">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6XPI8"/>
    <dbReference type="STRING" id="7719.ENSCINP00000005474"/>
    <dbReference type="Ensembl" id="ENSCINT00000005474.3">
        <property type="protein sequence ID" value="ENSCINP00000005474.3"/>
        <property type="gene ID" value="ENSCING00000002678.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000173516"/>
    <dbReference type="HOGENOM" id="CLU_2418630_0_0_1"/>
    <dbReference type="InParanoid" id="F6XPI8"/>
    <dbReference type="OMA" id="CGMEPTI"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008474">
        <property type="term" value="F:palmitoyl-(protein) hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050565">
        <property type="entry name" value="LYPA1-2/EST-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003140">
        <property type="entry name" value="PLipase/COase/thioEstase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10655:SF17">
        <property type="entry name" value="LYSOPHOSPHOLIPASE-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10655">
        <property type="entry name" value="LYSOPHOSPHOLIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02230">
        <property type="entry name" value="Abhydrolase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phospholipase/carboxylesterase/thioesterase" evidence="4">
        <location>
            <begin position="8"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000005474.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="92" mass="10009" checksum="AE65F2184C703A09" modified="2012-04-18" version="2">MSVSPQFFLHNSRVPATASVIFLHGLGDTGAGWYHGFDELRKNHVRYIFPNAPSISVTMNGGFVMPAWYDLKGLGPNTVEDKKGIEASAAKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>F6Y1Y0</accession>
    <name>F6Y1Y0_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">ATP-dependent DNA ligase family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">wild type</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent DNA ligase family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001718">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6Y1Y0"/>
    <dbReference type="STRING" id="7719.ENSCINP00000011712"/>
    <dbReference type="Ensembl" id="ENSCINT00000011712.3">
        <property type="protein sequence ID" value="ENSCINP00000011712.3"/>
        <property type="gene ID" value="ENSCING00000005638.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157783"/>
    <dbReference type="HOGENOM" id="CLU_110945_0_0_1"/>
    <dbReference type="InParanoid" id="F6Y1Y0"/>
    <dbReference type="OMA" id="CEARYLM"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003910">
        <property type="term" value="F:DNA ligase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3260.10">
        <property type="entry name" value="DNA ligase, ATP-dependent, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.30">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050191">
        <property type="entry name" value="ATP-dep_DNA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012310">
        <property type="entry name" value="DNA_ligase_ATP-dep_cent"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016059">
        <property type="entry name" value="DNA_ligase_ATP-dep_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012308">
        <property type="entry name" value="DNA_ligase_ATP-dep_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036599">
        <property type="entry name" value="DNA_ligase_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45674:SF4">
        <property type="entry name" value="DNA LIGASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45674">
        <property type="entry name" value="DNA LIGASE 1/3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01068">
        <property type="entry name" value="DNA_ligase_A_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04675">
        <property type="entry name" value="DNA_ligase_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117018">
        <property type="entry name" value="ATP-dependent DNA ligase DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56091">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00697">
        <property type="entry name" value="DNA_LIGASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA ligase ATP-dependent N-terminal" evidence="9">
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            <end position="45"/>
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        <location>
            <begin position="123"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000011712.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="217" mass="24234" checksum="44A72223CE0C488D" modified="2012-04-18" version="2">MSQKVSLVKSLLVACKHCEARYLMRSLGGKLRIGLAEQSVLVAIANAAYLTPPGQDFPPAIINAGEKIPAETLKSKMDEKALIVKTTYSELPNYDIVIKTMLEHGVDKLPDHCRLTPGIPLKPMLAHPTKGVSEVFNRFDKSEFTCEYKYDGERAQIHLLEGGEVRIYSRNQENNTSKYPDIISRVPAMLKDEVTSCVIDSEAVAWDTDKKQILPFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>F6Y4Z5</accession>
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        <recommendedName>
            <fullName evidence="4">ER membrane protein complex subunit 10</fullName>
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    <gene>
        <name evidence="12" type="primary">LOC100181673</name>
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    <organism evidence="12 13">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
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    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">wild type</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="3">Component of the ER membrane protein complex (EMC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EMC10 family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002030">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009858388.1">
        <property type="nucleotide sequence ID" value="XM_009860086.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6Y4Z5"/>
    <dbReference type="FunCoup" id="F6Y4Z5">
        <property type="interactions" value="75"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000019944"/>
    <dbReference type="Ensembl" id="ENSCINT00000019944.3">
        <property type="protein sequence ID" value="ENSCINP00000019944.3"/>
        <property type="gene ID" value="ENSCING00000009824.3"/>
    </dbReference>
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    <dbReference type="InParanoid" id="F6Y4Z5"/>
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    <dbReference type="OrthoDB" id="1894652at2759"/>
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        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072546">
        <property type="term" value="C:EMC complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd22209">
        <property type="entry name" value="EMC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21397">
        <property type="entry name" value="CHROMATIN COMPLEXES SUBUNIT BAP18-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21397:SF4">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21203">
        <property type="entry name" value="ECM10"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="6 11" id="KW-0732">Signal</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
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            <begin position="1"/>
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        </location>
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        <location>
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        </location>
    </feature>
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        <location>
            <begin position="197"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000019944.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="217" mass="24212" checksum="84F6CEEDE4DA11C1" modified="2012-04-18" version="2">MWCNPIFVLLFECSYLLSFAATSQDVGSGYLLSLEHSFGDTFTKRGDIYFPNVKGENAVVQNEIALSMEEIKELEKLAINDDFYRVRVSTKVLGAAPSSEDSYVVGIMPAKQLLEANLSTKIDILTDGIGNIISIGITPSSFGIMQVEENEFDSSVTVSMPTTATVPDTYGYLNKMEMERRKKEIADKQPTSFFGKYWMYILPVVVFVMMSNAAQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>F6Y7T8</accession>
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        <recommendedName>
            <fullName evidence="11">RRM domain-containing protein</fullName>
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        <name type="scientific">Ciona intestinalis</name>
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        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
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            <taxon>Chordata</taxon>
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            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
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    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">wild type</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the splicing factor SR family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001565">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6Y7T8"/>
    <dbReference type="FunCoup" id="F6Y7T8">
        <property type="interactions" value="23"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000014783"/>
    <dbReference type="Ensembl" id="ENSCINT00000014783.3">
        <property type="protein sequence ID" value="ENSCINP00000014783.3"/>
        <property type="gene ID" value="ENSCING00000011362.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156213"/>
    <dbReference type="HOGENOM" id="CLU_012062_34_2_1"/>
    <dbReference type="InParanoid" id="F6Y7T8"/>
    <dbReference type="OMA" id="DANRYRR"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000381">
        <property type="term" value="P:regulation of alternative mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12337">
        <property type="entry name" value="RRM1_SRSF4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000028">
        <property type="entry name" value="Putative serine/arginine-rich splicing factor 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:001067">
        <property type="entry name" value="Serine/arginine-rich splicing factor 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050374">
        <property type="entry name" value="RRT5_SRSF_SR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23003">
        <property type="entry name" value="RNA RECOGNITION MOTIF RRM DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23003:SF51">
        <property type="entry name" value="SERINE-ARGININE PROTEIN 55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 9" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="11">
        <location>
            <begin position="5"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="11">
        <location>
            <begin position="118"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="74"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="180"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="93"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="194"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="243"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000014783.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="283" mass="32127" checksum="8AA563B56A1A5DC0" modified="2012-04-18" version="2">MSYRSRVYIGRLSHRARENDVEHFFRGYGKINDLMLKNGFGFVIFDDERDADDAIHDLNGKSLCGERVMLEIAKGTPRGPGAPPDSRRESSSRRSSSSSYYQQQQPRSSTKGIPNHGYRLIVENLSSRVTWQDLKDYMRQCGEVVYADANRYRRNEGVVEFSSRKEMKYAIEKLNGTEINGRHIKLTPNIKSPSRSRSRSGSRGRKSGRYSRSRSRSGSRDKSKRSSSRSRSRSPKKAGKRSASKDNHNGVDKRKHSPHSDGSYRSRSRTGSPDGRKSASRSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>F6YHS8</accession>
    <name>F6YHS8_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">26S proteasome non-ATPase regulatory subunit 12</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">26S proteasome regulatory subunit RPN5</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">wild type</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the 19S proteasome regulatory particle complex. The 26S proteasome consists of a 20S core particle (CP) and two 19S regulatory subunits (RP). The regulatory particle is made of a lid composed of 9 subunits including PSMD12, a base containing 6 ATPases and few additional components. Interacts with ERCC6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the proteasome subunit p55 family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01000145">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6YHS8"/>
    <dbReference type="FunCoup" id="F6YHS8">
        <property type="interactions" value="959"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000010751"/>
    <dbReference type="Ensembl" id="ENSCINT00000010751.3">
        <property type="protein sequence ID" value="ENSCINP00000010751.3"/>
        <property type="gene ID" value="ENSCING00000005232.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153510"/>
    <dbReference type="HOGENOM" id="CLU_033860_2_0_1"/>
    <dbReference type="InParanoid" id="F6YHS8"/>
    <dbReference type="OMA" id="AENEMFK"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008541">
        <property type="term" value="C:proteasome regulatory particle, lid subcomplex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000159">
        <property type="entry name" value="26S proteasome non-ATPase regulatory subunit 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054559">
        <property type="entry name" value="PSMD12-CSN4-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040134">
        <property type="entry name" value="PSMD12/CSN4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040896">
        <property type="entry name" value="RPN5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10855:SF1">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10855">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 12/COP9 SIGNALOSOME COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22241">
        <property type="entry name" value="PSMD12-CSN4_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18098">
        <property type="entry name" value="RPN5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="5" id="KW-0647">Proteasome</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="PCI" evidence="10">
        <location>
            <begin position="242"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00065326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00068189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00075108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000010751.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="458" mass="53271" checksum="D21C1DDB4E5E3737" modified="2012-04-18" version="2">IQFKMSGDGKLEKMEVDYSETVDKLIPECETIAKDGNLEEALEKLYGLEKQTRVACDMVSNGKIMVCAVKICFEAKKWEMLNDIIVVLTKKRSQLKLAVAKMVQECYTYVEKTPDLETKLKLIDTLRVVTEGKIYVENERARLTMKLAKIKENQGDIGEAATILQELQVETFGSMERKEKIEFILEQMRLCLAKKDYIRVQIISKKISIRFFENKDEDVQQLKLRYYQMMIELDLADGNYLSTCKHFKAVYDTPLIETDKIKWKQALKSVVLYVILAPHDNEQSDMLERVRTEKKLEEIPKYKELLECFVSQELMQLEKFMALYEHELREGESGSPCTDVFLHTEDGQKRWKDFANRVVEHNIRIMAKYYTRITSKRMSNLLDLSVEEAEEYLAKLVVNKTVYAKIDRLAGVVTFTRPRDPSDVLNEWSHSVNKLMGLVNKATHLIAKEEMIHSLKAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>F6YVH4</accession>
    <name>F6YVH4_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">unspecific monooxygenase</fullName>
            <ecNumber evidence="5">1.14.14.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">wild type</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Microsome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EC" id="1.14.14.1" evidence="5"/>
    <dbReference type="EMBL" id="EAAA01002101">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="F6YVH4"/>
    <dbReference type="STRING" id="7719.ENSCINP00000012842"/>
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        <property type="protein sequence ID" value="ENSCINP00000012842.3"/>
        <property type="gene ID" value="ENSCING00000006203.3"/>
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    <dbReference type="GeneTree" id="ENSGT00950000183037"/>
    <dbReference type="HOGENOM" id="CLU_001570_22_0_1"/>
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        <property type="component" value="Chromosome 5"/>
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        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen"/>
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        <property type="term" value="P:estrogen metabolic process"/>
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        <property type="term" value="P:steroid catabolic process"/>
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        <property type="term" value="P:toxin metabolic process"/>
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    <dbReference type="GO" id="GO:0042178">
        <property type="term" value="P:xenobiotic catabolic process"/>
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        <property type="project" value="GO_Central"/>
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        <property type="entry name" value="CYP1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="cytochrome P450 2J6-like"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="1.10.630.10">
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        <property type="entry name" value="Cyt_P450"/>
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        <property type="entry name" value="Cyt_P450_CS"/>
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        <property type="entry name" value="Cyt_P450_E_grp-I"/>
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    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
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    <dbReference type="PANTHER" id="PTHR24289:SF21">
        <property type="entry name" value="CYTOCHROME P450 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24289">
        <property type="entry name" value="STEROID 17-ALPHA-HYDROXYLASE/17,20 LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EP450I"/>
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        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 13 14" id="KW-0349">Heme</keyword>
    <keyword evidence="11 13 14" id="KW-0408">Iron</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
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    <keyword evidence="12 14" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="10 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="13">
        <location>
            <position position="457"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000012842.3"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>F6ZAA5</accession>
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        <recommendedName>
            <fullName evidence="3">Sulfotransferase</fullName>
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    <organism evidence="5 6">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
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            <taxon>Ciona</taxon>
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    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">wild type</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the sulfotransferase 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="3"/>
    <dbReference type="EMBL" id="EAAA01002439">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6ZAA5"/>
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        <property type="protein sequence ID" value="ENSCINP00000014736.3"/>
        <property type="gene ID" value="ENSCING00000007185.3"/>
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    <dbReference type="HOGENOM" id="CLU_027239_1_2_1"/>
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    <dbReference type="OMA" id="IVETHES"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051923">
        <property type="term" value="P:sulfation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11783">
        <property type="entry name" value="SULFOTRANSFERASE SULT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Sulfotransferase" evidence="4">
        <location>
            <begin position="5"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000014736.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="259" mass="30482" checksum="ACED641708785D34" modified="2012-04-18" version="2">MPWKNDVIIAAYAKTGTTWVRNIVSHLIYRQNPQAMEMYKNLHAFLVYLETGTLLDKLPWKRRILATHIPAKLLNMKRIKGSGAKIICPIRNPKDQIVSWYNMAKKLPVPKNHELVKSLYPRTWEEFIEVYTQGQQPLASKVGEWYPDYLLSWYQHKEDENVLFVVYEDMKKDPVKEIQKIADFLDIPISESDLDEVVHETSFNTMRRQSAQIEKKLDFYRKGEVGDWKNHLTVAQSEMIDAKIQEKLGHTDLKFTYEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>F6ZBN8</accession>
    <name>F6ZBN8_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CAP-Gly domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01001168">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6ZBN8"/>
    <dbReference type="STRING" id="7719.ENSCINP00000026477"/>
    <dbReference type="Ensembl" id="ENSCINT00000026723.2">
        <property type="protein sequence ID" value="ENSCINP00000026477.2"/>
        <property type="gene ID" value="ENSCING00000023530.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000168425"/>
    <dbReference type="HOGENOM" id="CLU_083201_0_0_1"/>
    <dbReference type="InParanoid" id="F6ZBN8"/>
    <dbReference type="OMA" id="SCKERYE"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.190">
        <property type="entry name" value="CAP Gly-rich-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036859">
        <property type="entry name" value="CAP-Gly_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000938">
        <property type="entry name" value="CAP-Gly_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01302">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01052">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74924">
        <property type="entry name" value="Cap-Gly domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50245">
        <property type="entry name" value="CAP_GLY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CAP-Gly" evidence="1">
        <location>
            <begin position="143"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000026477.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="292" mass="33008" checksum="8B99E6B17940D67F" modified="2012-04-18" version="2">MAERYSRDMIPTRYLILVNDLCFTKKNVPYRPKKFESHIQEYLKGTILQEVFFASMPTTNTTRITCIVLDTREIIECDENDVDALTEEIMGLLMAISSCKERYDVFRNKERLADGLDMEAGAEVVVRLPAMSGEVLGRLRYKGKVHHKVGTYFGVELINAKGKGNTNGTIRKHHYFSCPEDCGIFLGLNRVSSRDYDQLRTPIPAPRRHPRSQRSMISNSYSDLSATRDYSSPPSGSSQVSVCEPVIGVSEAELSTLNIHIDMKVYIFTEVNGHESQELGSVKFIGRLPNEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>F6ZJB9</accession>
    <name>F6ZJB9_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">MICOS complex subunit MIC60</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Mitofilin</fullName>
        </alternativeName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the mitochondrial contact site and cristae organizing system (MICOS) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Mitochondrion inner membrane</location>
            <topology evidence="7">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the MICOS complex subunit Mic60 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="F6ZJB9"/>
    <dbReference type="FunCoup" id="F6ZJB9">
        <property type="interactions" value="171"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000027819"/>
    <dbReference type="Ensembl" id="ENSCINT00000028065.2">
        <property type="protein sequence ID" value="ENSCINP00000027819.2"/>
        <property type="gene ID" value="ENSCING00000015859.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000002313"/>
    <dbReference type="HOGENOM" id="CLU_021851_2_0_1"/>
    <dbReference type="InParanoid" id="F6ZJB9"/>
    <dbReference type="OMA" id="TNCADIE"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061617">
        <property type="term" value="C:MICOS complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042407">
        <property type="term" value="P:cristae formation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019133">
        <property type="entry name" value="MIC60"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15415:SF7">
        <property type="entry name" value="MICOS COMPLEX SUBUNIT MIC60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15415">
        <property type="entry name" value="MITOFILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09731">
        <property type="entry name" value="Mitofilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="73"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="135"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000027819.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="519" mass="58782" checksum="FD458973090721CE" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>F6ZL08</accession>
    <name>F6ZL08_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">HTTM-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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    <reference evidence="9" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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            <strain evidence="9">wild type</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Chapman J."/>
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                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
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                <person name="Putnam N."/>
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                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
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                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
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                <person name="Makabe K.W."/>
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                <person name="Yoshizaki F."/>
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                <person name="Hyatt P.D."/>
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                <person name="Richardson P."/>
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    <sequence length="308" mass="34551" checksum="B51EBF0B0E3568DB" modified="2012-04-18" version="2">ETEPLYDRVDDVTMAKPTSPPSGDSTQELFHGSYARLYAQKPDARPRYQRQASTTSNYRSRAYSATSRASGGGGTLRKTIVHKNAERHLRISRTVLTYVCVFAVCWLPQRIVAVVYVVEGMVGLGGHACHAVFTATRILSFLSVLLNPIVYAMTQREIRHFLRRKLAGMCKCIHPGRESNPDPQNRDVNVSFANARRDFSVVADHNITSDEISMKRERHARNKERWSRLMHRLGGKNLRLSLTGSGFTSPGEKQSDGTIDEDEEKGQSSKGQSSSTSFKLIGGWEDWQQMIDMAEVNKGQTTNEETIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>F7AA68</accession>
    <accession>A0A1W2W1F3</accession>
    <name>F7AA68_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Sperm-associated antigen 8-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC100177416</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="RefSeq" id="XP_002121318.1">
        <property type="nucleotide sequence ID" value="XM_002121282.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F7AA68"/>
    <dbReference type="STRING" id="7719.ENSCINP00000016212"/>
    <dbReference type="Ensembl" id="ENSCINT00000016212.3">
        <property type="protein sequence ID" value="ENSCINP00000016212.3"/>
        <property type="gene ID" value="ENSCING00000007919.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100177416"/>
    <dbReference type="KEGG" id="cin:100177416"/>
    <dbReference type="GeneTree" id="ENSGT00640000091617"/>
    <dbReference type="HOGENOM" id="CLU_118848_0_0_1"/>
    <dbReference type="InParanoid" id="F7AA68"/>
    <dbReference type="OMA" id="EYCSTTQ"/>
    <dbReference type="OrthoDB" id="2120499at2759"/>
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        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026124">
        <property type="entry name" value="Sperm-assoc_Ag8"/>
    </dbReference>
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        <property type="entry name" value="SPERM-ASSOCIATED ANTIGEN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15510:SF5">
        <property type="entry name" value="SPERM-ASSOCIATED ANTIGEN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22584">
        <property type="entry name" value="CFAP143"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="188"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000016212.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="201" mass="23224" checksum="C5E4B6C117B0C7C3" modified="2011-07-27" version="1">MSLFEGRNEIRFNNSGGKCLLENWVEERATASLEPTDVNSECTSSAQLHRHGHKGLLTLSLDANAEKLTTVRESYQKPKFPEVRTRGKKEEMIERMLYNKVGEETHREFNPPPPPMEYVTIKQQDFDKPDFKSTVPPPTKDHNVNTDQCVTYWSTHTNKIHGVSQVKTRDTPFKKNTAFSKPISEYLDQTKPHDEEQYPNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>F7ACY4</accession>
    <name>F7ACY4_CIOIN</name>
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        <recommendedName>
            <fullName evidence="8">Telomere length and silencing protein 1 homolog</fullName>
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    </protein>
    <organism evidence="6 7">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
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            <taxon>Phlebobranchia</taxon>
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            <taxon>Ciona</taxon>
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    <reference evidence="7" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">wild type</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TLS1 family.</text>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="STRING" id="7719.ENSCINP00000019545"/>
    <dbReference type="Ensembl" id="ENSCINT00000019545.3">
        <property type="protein sequence ID" value="ENSCINP00000019545.3"/>
        <property type="gene ID" value="ENSCING00000009616.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000009787"/>
    <dbReference type="HOGENOM" id="CLU_053736_1_0_1"/>
    <dbReference type="InParanoid" id="F7ACY4"/>
    <dbReference type="OMA" id="NIKTGGM"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010756">
        <property type="entry name" value="Tls1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13486:SF2">
        <property type="entry name" value="SPLICING FACTOR C9ORF78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13486">
        <property type="entry name" value="TELOMERE LENGTH AND SILENCING PROTEIN 1 TLS1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07052">
        <property type="entry name" value="Hep_59"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
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            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="233"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
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            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="233"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="256"/>
            <end position="288"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000019545.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
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                <person name="Doyle S."/>
                <person name="Francino P."/>
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                <person name="Kobayashi K."/>
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                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
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                <person name="Richardson P."/>
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                <person name="Kohara Y."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Makabe K.W."/>
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                <person name="Mochizuki Y."/>
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                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
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                <person name="Zhang C."/>
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                <person name="Doggett N."/>
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                <person name="Richardson P."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
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            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">wild type</strain>
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    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
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                <consortium name="Ensembl"/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
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                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
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                <person name="Haga S."/>
                <person name="Hayashi H."/>
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                <person name="Imai K.S."/>
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                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
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                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
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                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
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                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
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                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
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                <person name="Kobayashi K."/>
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                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
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                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
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                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
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                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
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                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
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            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="12">wild type</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <text evidence="3">Belongs to the GORAB family.</text>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">wild type</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a di-trans,poly-cis-dolichyl phosphate + UDP-alpha-D-glucose = a di-trans,poly-cis-dolichyl beta-D-glucosyl phosphate + UDP</text>
            <dbReference type="Rhea" id="RHEA:15401"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19502"/>
            <dbReference type="ChEBI" id="CHEBI:57525"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.4.1.117"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:15402"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyltransferase 2 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.117" evidence="4"/>
    <dbReference type="EMBL" id="EAAA01000434">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F7B391"/>
    <dbReference type="FunCoup" id="F7B391">
        <property type="interactions" value="1234"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000005302"/>
    <dbReference type="Ensembl" id="ENSCINT00000005302.3">
        <property type="protein sequence ID" value="ENSCINP00000005302.3"/>
        <property type="gene ID" value="ENSCING00000002601.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153481"/>
    <dbReference type="HOGENOM" id="CLU_033536_9_1_1"/>
    <dbReference type="InParanoid" id="F7B391"/>
    <dbReference type="OMA" id="HMVNTDA"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004581">
        <property type="term" value="F:dolichyl-phosphate beta-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04188">
        <property type="entry name" value="DPG_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035518">
        <property type="entry name" value="DPG_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10859:SF91">
        <property type="entry name" value="DOLICHYL-PHOSPHATE BETA-GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10859">
        <property type="entry name" value="GLYCOSYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="13">
        <location>
            <begin position="70"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000005302.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="322" mass="36834" checksum="17FBB1614C15368E" modified="2025-10-08" version="3">MHCYICILYYILVIQSNGILLNDRLYIVCHVTVSTMATYLRYESENYFKNSKGEKERFPSLMDEESVDLSVIVPSYNEEKRLPLMMEEAILFLEAKCEKSSNYKYEIIIVDDGSKDSTTKVGQSFVEKYGSEKIRVLTLEKNRGKGGAVRLGMMSARGEHLLFADADGATKFSDIEKVETKLNDIDTKPHGRALTVGSRAHLEKNSIASRSVFRTFLMKCFHLIVWLLCARSVKDSQCGFKLFTRNAARILFHNLHVERWAFDVELLYVAEELNIPIAEVAVTWTEIDGSKVDPLFSGLQMGKDIVLLWLQYQLGLWVIKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>F7B7M7</accession>
    <name>F7B7M7_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">PX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="F7B7M7"/>
    <dbReference type="FunCoup" id="F7B7M7">
        <property type="interactions" value="80"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000027235"/>
    <dbReference type="Ensembl" id="ENSCINT00000027481.2">
        <property type="protein sequence ID" value="ENSCINP00000027235.2"/>
        <property type="gene ID" value="ENSCING00000015325.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000175582"/>
    <dbReference type="HOGENOM" id="CLU_020563_1_1_1"/>
    <dbReference type="InParanoid" id="F7B7M7"/>
    <dbReference type="OMA" id="TIPHVKL"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07277">
        <property type="entry name" value="PX_RUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004012">
        <property type="entry name" value="Run_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037213">
        <property type="entry name" value="Run_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037916">
        <property type="entry name" value="SNX29_PX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47194:SF3">
        <property type="entry name" value="SORTING NEXIN 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47194">
        <property type="entry name" value="SORTING NEXIN-29-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02759">
        <property type="entry name" value="RUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00593">
        <property type="entry name" value="RUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140741">
        <property type="entry name" value="RUN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50826">
        <property type="entry name" value="RUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RUN" evidence="3">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="PX" evidence="2">
        <location>
            <begin position="304"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="119"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="121"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000027235.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="423" mass="48454" checksum="B7D302758176D453" modified="2012-04-18" version="2">EITFWNFIKNHLSKDEIERFENLTNIRSDIGRGRAWLRCSLNEHSLERYFYIFLSDPDILRQYYEEEALLLDDEISSTLPSMAAGLASIYFSINFDVASLDGSAIKPSSSSLHMEEIVSPTRESATPSVGTHTSSGTHASESELRQTLIVTLQAKDEILEKNRSMRKLLEAEMLQTSSLRKENGKLLEEVARGKMAQVEAGRENEVLRSQLKKYVSAVQTMSVEDVSGGEEVYERKLVEMAGMHAELVEFNEHIHTRLKSAINLLRYMRSELVDLRGPMPTDDLVGRIEADDIIGILESSSSPARISVWMPSVLLKGRGPDASHFYQVFIRAGDDEWNVYRRYSEFHEFHLQIAKKFPVVETFNFPPKKLVGNKSRQFVEERRRSLQSYIRLMINNIIRNHEKIISNPTKTTLVTLLPFFSET</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>F7B7S8</accession>
    <name>F7B7S8_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">Carbonic anhydrase</fullName>
            <ecNumber evidence="2 6">4.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100177506</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">wild type</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Reversible hydration of carbon dioxide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>hydrogencarbonate + H(+) = CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:10748"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="EC" id="4.2.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the alpha-carbonic anhydrase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.1" evidence="2 6"/>
    <dbReference type="EMBL" id="EAAA01001126">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F7B7S8"/>
    <dbReference type="STRING" id="7719.ENSCINP00000003284"/>
    <dbReference type="Ensembl" id="ENSCINT00000003284.3">
        <property type="protein sequence ID" value="ENSCINP00000003284.3"/>
        <property type="gene ID" value="ENSCING00000014543.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000167899"/>
    <dbReference type="HOGENOM" id="CLU_039326_1_2_1"/>
    <dbReference type="InParanoid" id="F7B7S8"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004089">
        <property type="term" value="F:carbonate dehydratase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.200.10:FF:000003">
        <property type="entry name" value="Carbonic anhydrase 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.200.10">
        <property type="entry name" value="Alpha carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001148">
        <property type="entry name" value="CA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036398">
        <property type="entry name" value="CA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023561">
        <property type="entry name" value="Carbonic_anhydrase_a-class"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018338">
        <property type="entry name" value="Carbonic_anhydrase_a-class_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952:SF124">
        <property type="entry name" value="CARBONIC ANHYDRASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00194">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01057">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51069">
        <property type="entry name" value="Carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00162">
        <property type="entry name" value="ALPHA_CA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51144">
        <property type="entry name" value="ALPHA_CA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025086613" description="Carbonic anhydrase" evidence="6">
        <location>
            <begin position="18"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-carbonic anhydrase" evidence="7">
        <location>
            <begin position="18"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000003284.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="305" mass="33944" checksum="9DE2BBD64DEB22DF" modified="2012-04-18" version="2">MKVTVIALLLVIDQAIGATWVYSQGPKGPSNWLNSYAACGTRAQSPISIEHENSVANKNLGTFDRSVFTSLPASMELVNNGHALQVNMDGSYTISDRSVLPNDYKAVQFHLHWAAANKSEGSEHWLNGKQYFAELHVVHYNTKYASIGEAVNKPDGLAVLGVFVDINDETNEHFNLLLNPINRVQIPNQEMTYTSKFSVANFLPTDLSEYYRYRGSLTTPPCFDSVVWTVFKEPIHMSRDQARVLQTSLYDGINSTAKVGGNFRLPQPINGREVSRPYKSGANPIPLPIFLQITAMLAVVKTLYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>F7B7Y1</accession>
    <name>F7B7Y1_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Dedicator of cytokinesis protein 7</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">wild type</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the DOCK family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001377">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="F7B7Y1">
        <property type="interactions" value="266"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000015250"/>
    <dbReference type="Ensembl" id="ENSCINT00000015250.3">
        <property type="protein sequence ID" value="ENSCINP00000015250.3"/>
        <property type="gene ID" value="ENSCING00000007422.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159313"/>
    <dbReference type="HOGENOM" id="CLU_000624_0_0_1"/>
    <dbReference type="InParanoid" id="F7B7Y1"/>
    <dbReference type="OMA" id="TFYHLGC"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035023">
        <property type="term" value="P:regulation of Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08696">
        <property type="entry name" value="C2_Dock-C"/>
        <property type="match status" value="1"/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
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                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
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                <person name="Harafuji N."/>
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                <person name="Ho I."/>
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                <person name="Huang W."/>
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                <person name="Martinez D."/>
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                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
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                <person name="Suzuki M.M."/>
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                <person name="Zhang C."/>
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                <person name="Richardson P."/>
                <person name="Lucas S."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4">wild type</strain>
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        <scope>IDENTIFICATION</scope>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
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                <person name="Wiley G.B."/>
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                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="5" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">wild type</strain>
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    <reference evidence="5" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
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    <evidence type="ECO:0000313" key="2">
        <source>
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        <source>
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        <source>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
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                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
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                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
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                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
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                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
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                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        </citation>
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    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
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    </reference>
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    <dbReference type="Ensembl" id="ENSCINT00000036082.1">
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    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    <sequence length="113" mass="12573" checksum="4D885B61AFAE63F3" modified="2012-04-18" version="1">ISVVDNPLTEEDLVDYLENTLRTSFNITADAAKYCNKIISKSYDLPILPPPSQPSTTGSTIELMEAISESNSTDLLNAMEVLSHEVPHSDEDSFEFVPDFKISHQLLNEPLPH</sequence>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01002671">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XKC7"/>
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            <dbReference type="Ensembl" id="ENSCINP00000030109.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    </evidence>
    <sequence length="352" mass="40877" checksum="552543CD07384DB0" modified="2012-04-18" version="1">VLLLHTRIFVFKITLFSLQAEDEKPKISLNMDPVDVKTWGLPLDSKYPDVVQINEANFKPKVLESRDAWIVVVFKDKLHGKWWHHAGHVKGAVWYGSVDIEKQENFAKLLQQAPESTAENIGNGVAVIFPFGVTPKEKIKFTGKKKKRPLIVKSPTKAEEIISSSIPNQIERMNFKPSRMKKFQDWVVDSYYKSSPPRFPVLCITDEPEPTIPVSMLVLSNYFSRFFSFALVRKEDVSRMRLQFKEVPEPKVYPHYLMLMGKEPSKEEMDRGSFGMHFNIFPFLADKYGRSELFTSVVKFLFTANEDYRNELPGRRSDEAISEARMSLVRDSLRNRVELVNKHFRKQKNVEL</sequence>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
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                <person name="Huang W."/>
                <person name="Kawashima T."/>
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                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Putnam N."/>
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                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
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                <person name="Doyle S."/>
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                <person name="Hayashi H."/>
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                <person name="Inaba K."/>
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                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
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                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
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                <person name="Nomoto H."/>
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                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
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                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
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            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <property type="protein sequence ID" value="ENSCINP00000030139.1"/>
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    </evidence>
    <sequence length="86" mass="9831" checksum="D0722C78E017808C" modified="2012-04-18" version="1">MFYLGEDENWYLDRFGILFTNNSRYNNTAVLFDSPRALLRVTAYVTYLSSHGGATTVYNTGVLLHTPCACLQVTSYVTLWVILHNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>H2XKI4</accession>
    <name>H2XKI4_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">wild type</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01001449">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XKI4"/>
    <dbReference type="Ensembl" id="ENSCINT00000032318.1">
        <property type="protein sequence ID" value="ENSCINP00000030166.1"/>
        <property type="gene ID" value="ENSCING00000020360.1"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1776799_0_0_1"/>
    <dbReference type="InParanoid" id="H2XKI4"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000030166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="146" mass="16686" checksum="0B41A2C6D8327480" modified="2012-04-18" version="1">MTAKLSKYEKRLCILLFLSTMTNPLSTLSTLFSHYTPDHHCKIQYPQNEFSSSNISVVSSLKNKYLEVAIPDEEPNLFGRRGMSRCYEYNMTTEKQQSVLKKLRNGTVEYISNRSMSENIVTCKHGSTFNFKDGETSAIYEVICGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>H2XKZ3</accession>
    <name>H2XKZ3_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01000228">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XKZ3"/>
    <dbReference type="Ensembl" id="ENSCINT00000031328.1">
        <property type="protein sequence ID" value="ENSCINP00000030325.1"/>
        <property type="gene ID" value="ENSCING00000023514.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00930000151043"/>
    <dbReference type="HOGENOM" id="CLU_1431033_0_0_1"/>
    <dbReference type="InParanoid" id="H2XKZ3"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051177">
        <property type="entry name" value="CIK-Related_Protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12984:SF15">
        <property type="entry name" value="PROTEIN-ASSOCIATING WITH THE CARBOXYL-TERMINAL DOMAIN OF EZRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12984">
        <property type="entry name" value="SCY1-RELATED S/T PROTEIN KINASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="12"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000030325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="190" mass="21587" checksum="D1A9505452D41F15" modified="2012-04-18" version="1">MWNAAWDWYYGSNDEEIFADCKFNKAYEGYDGKKTSKGKLLIRHGTLANGDSVNVFTKVWRKNKSPCPLDLCAKLLRVLRHPNILRFVGCIPVESEIHLITELASPISTIDDLDANEICSGLYDIALALQFMHDKAKVSHNNVRFESIYVTKDGVWKLGDFEHACPFAEANEEQMKVAYKSNSIIPPEEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>H2XL56</accession>
    <name>H2XL56_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CUB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
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                <person name="Martinez D."/>
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                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
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                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
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                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
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                <person name="Suzuki M.M."/>
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                <person name="Yoshizaki F."/>
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                <person name="Zhang C."/>
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                <person name="Larimer F."/>
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                <person name="Doggett N."/>
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                <person name="Richardson P."/>
                <person name="Lucas S."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
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                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">wild type</strain>
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    <reference evidence="5" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <text evidence="1">Belongs to the CCZ1 family.</text>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satake M."/>
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                <person name="Wang H.G."/>
                <person name="Awazu S."/>
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                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
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    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="80"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="383"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000031778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="441" mass="49124" checksum="FB6936B58CBA94C5" modified="2012-04-18" version="1">MGSHNKRMKKRRELDALLSGGRSKQGYEQLQPQSTSHHADIVFSSSSRDGDNFNSHLNYGTRKMGRAISIRKRACCMKRLYHICLPACMVAMIVTCVVISVGIVWLQLRLKSEVDGVKMRISKLEGWSRDEPASLADLETLVSSMQLEVTNIKQQIIQPLQLKLAGLMHQVEQQSSRQITQTFQDSINTLQQNVVELTTDVTHVHQSVTGLETSTQQLHAQTTQNSDDIARISSLVTDYKQEIESMENKPQRYAMASTSEGFYNSSINAIQSRLKLLEDNSATTNMLQPLSSKQTLMEVEISIIKNTVASVNESIMTLLDDNHGLQTQISSLEQTTENLRQSFLNEGESLSLPLTPETGGSNLNPQNIETNANVENVLNESVFPSHGTDHRVGDTTTSFTELPTKGQPEENDTVVGNLNELTEVATDYENSRTDLSHKTET</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>H2XRA0</accession>
    <name>H2XRA0_CIOIN</name>
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        <recommendedName>
            <fullName evidence="11">CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase</fullName>
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    </protein>
    <organism evidence="13 14">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">wild type</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 11">Functions in the biosynthesis of the anionic phospholipids phosphatidylglycerol and cardiolipin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>a CDP-1,2-diacyl-sn-glycerol + sn-glycerol 3-phosphate = a 1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycero-3'-phosphate) + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12593"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:58332"/>
            <dbReference type="ChEBI" id="CHEBI:60110"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="EC" id="2.7.8.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Phospholipid metabolism; phosphatidylglycerol biosynthesis; phosphatidylglycerol from CDP-diacylglycerol: step 1/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the CDP-alcohol phosphatidyltransferase class-II family.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.5" evidence="11"/>
    <dbReference type="EMBL" id="EAAA01000446">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XRA0"/>
    <dbReference type="STRING" id="7719.ENSCINP00000032184"/>
    <dbReference type="Ensembl" id="ENSCINT00000035013.1">
        <property type="protein sequence ID" value="ENSCINP00000032184.1"/>
        <property type="gene ID" value="ENSCING00000017979.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000002373"/>
    <dbReference type="HOGENOM" id="CLU_030471_1_2_1"/>
    <dbReference type="InParanoid" id="H2XRA0"/>
    <dbReference type="OMA" id="WLWDARY"/>
    <dbReference type="UniPathway" id="UPA00084">
        <property type="reaction ID" value="UER00503"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008444">
        <property type="term" value="F:CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032049">
        <property type="term" value="P:cardiolipin biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd09135">
        <property type="entry name" value="PLDc_PGS1_euk_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09137">
        <property type="entry name" value="PLDc_PGS1_euk_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Endonuclease Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="PGS1"/>
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        <property type="entry name" value="PLipase_D/transphosphatidylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12586:SF1">
        <property type="entry name" value="CDP-DIACYLGLYCEROL--GLYCEROL-3-PHOSPHATE 3-PHOSPHATIDYLTRANSFERASE, MITOCHONDRIAL"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR12586">
        <property type="entry name" value="CDP-DIACYLGLYCEROL--SERINE O-PHOSPHATIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Phospholipase_D_PSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56024">
        <property type="entry name" value="Phospholipase D/nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50035">
        <property type="entry name" value="PLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 11" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="7 11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 11" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="9 11" id="KW-1208">Phospholipid metabolism</keyword>
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    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PLD phosphodiesterase" evidence="12">
        <location>
            <begin position="133"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003537"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>H2XRB6</accession>
    <name>H2XRB6_CIOIN</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
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    <organism evidence="1 2">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
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            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="H2XRB6"/>
    <dbReference type="Ensembl" id="ENSCINT00000034310.1">
        <property type="protein sequence ID" value="ENSCINP00000032200.1"/>
        <property type="gene ID" value="ENSCING00000025171.1"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2351906_0_0_1"/>
    <dbReference type="InParanoid" id="H2XRB6"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000032200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="97" mass="10650" checksum="FC1245152BA92AB8" modified="2012-04-18" version="1">CEPPIVSGRCLAPNRYNSQPPLYLSKIVVVSYCNRPAALREIVACCSRLSLEVLSICTLGMRPLSCSKSSHFAGASLLRASLYANVIWEKHWLKALG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>H2XRQ4</accession>
    <name>H2XRQ4_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="H2XRQ4"/>
    <dbReference type="Ensembl" id="ENSCINT00000031967.1">
        <property type="protein sequence ID" value="ENSCINP00000032338.1"/>
        <property type="gene ID" value="ENSCING00000017904.1"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3224251_0_0_1"/>
    <dbReference type="InParanoid" id="H2XRQ4"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000032338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="44" mass="5020" checksum="EE6E815F4F6384CD" modified="2012-04-18" version="1">MLKANVKSRKCVITSSIAHSNLYHSVTQINRKLINTSMLVRSYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>H2XS53</accession>
    <name>H2XS53_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Arsenite methyltransferase</fullName>
            <ecNumber evidence="4">2.1.1.137</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100179278</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>arsenic triglutathione + 2 [thioredoxin]-dithiol + 2 S-adenosyl-L-methionine + H2O = dimethylarsinous acid + 2 [thioredoxin]-disulfide + 3 glutathione + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:69464"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:23808"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:183640"/>
            <dbReference type="EC" id="2.1.1.137"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>arsenic triglutathione + 3 [thioredoxin]-dithiol + 3 S-adenosyl-L-methionine = trimethylarsine + 3 [thioredoxin]-disulfide + 3 glutathione + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:69432"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:27130"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:183640"/>
            <dbReference type="EC" id="2.1.1.137"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>arsenic triglutathione + [thioredoxin]-dithiol + S-adenosyl-L-methionine + 2 H2O = methylarsonous acid + [thioredoxin]-disulfide + 3 glutathione + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:69460"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17826"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:183640"/>
            <dbReference type="EC" id="2.1.1.137"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the methyltransferase superfamily. Arsenite methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.137" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="H2XS53"/>
    <dbReference type="STRING" id="7719.ENSCINP00000032487"/>
    <dbReference type="Ensembl" id="ENSCINT00000031051.1">
        <property type="protein sequence ID" value="ENSCINP00000032487.1"/>
        <property type="gene ID" value="ENSCING00000022553.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000001742"/>
    <dbReference type="HOGENOM" id="CLU_052868_0_1_1"/>
    <dbReference type="InParanoid" id="H2XS53"/>
    <dbReference type="OMA" id="EPACEDY"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030791">
        <property type="term" value="F:arsenite methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018872">
        <property type="term" value="P:arsonoacetate metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009404">
        <property type="term" value="P:toxin metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.5.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026669">
        <property type="entry name" value="Arsenite_MeTrfase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025714">
        <property type="entry name" value="Methyltranfer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43675">
        <property type="entry name" value="ARSENITE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43675:SF8">
        <property type="entry name" value="ARSENITE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13847">
        <property type="entry name" value="Methyltransf_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase" evidence="9">
        <location>
            <begin position="74"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00034487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00034521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00034545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000032487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="316" mass="35552" checksum="7C016D1881EFF296" modified="2012-04-18" version="1">MSCCPAPNITDEVKEYYGKTLQSTNDLQTSMCTTPPQAVAKHVREALKLVHNDVTSKYYGYVSYVAIRNNPTPKLLDLGSGSGRDCYALAKLVGEDGFVTGVDMTDEQLAVANKYIDYHQQSFGYSKPNTKFVKGFIEKLGEAGIEDTSMDIVISNCVINLVKDKEAVLKEAYRVLKYGGELYFSDVYADCVLSEEIRNHKVLWGECVSGALYWKELYEIAESVGFETPRVVSANVVEVTKPELKKVTGDARFASVLYRLFKESTQVVYNGGITGYEENFKLDREYFFTRDTPTNVDPVTSQILKQSRFNEEFSMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>H2XSC5</accession>
    <name>H2XSC5_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Carboxypeptidase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
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                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S10 family.</text>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Chapman J."/>
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                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
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                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
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                <person name="Hyatt P.D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
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                <person name="Doyle S."/>
                <person name="Francino P."/>
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                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
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                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
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                <person name="Miura S."/>
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                <person name="Nishizaka S."/>
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                <person name="Ohta F."/>
                <person name="Oishi K."/>
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                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
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                <person name="Suzuki M.M."/>
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                <person name="Yoshizaki F."/>
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                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>IDENTIFICATION</scope>
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                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
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                <person name="Satake M."/>
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                <person name="Awazu S."/>
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                <person name="Mount S."/>
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                <person name="Tassy O."/>
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                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
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                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
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                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
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                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
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                <person name="De Tomaso A."/>
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                <person name="Gelpke M."/>
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                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
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                <person name="Terry A."/>
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                <person name="Ueno K."/>
                <person name="Yamada L."/>
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                <person name="Wasserscheid J."/>
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        </source>
    </evidence>
    <sequence length="511" mass="56744" checksum="0D371718C126F61A" modified="2012-04-18" version="1">EPYTCTGNFPDDFAELWQRSGLDKEHVPVVKPRAKRPATPTADDAKTTPVNDQKEKNYGCFALAQNGFTNRYHKNVTVRQVEFDVEDTKQMQINHAKELFIRSWKVEEKVLGILKMCILERLQCINLWNVGLTEHTLKILSSLLPSLPSIKSLVLDGNPIPGSEPYYLVMGEDSPIQHLSLRNNQITDQGAEMIGKALGTTFTQNRCLATLNLSFNNISDAGAKHIAQGIRMNRVLLSLSLASNNISDEGCQYICNALQRFSLTHEEVVQRRKLLSQGGFRGGSGLGSRRTDSKDRPSSNRSGSQMRTSRGSSKKKPDAGKKDAEKTPKKDEKGKKGEIKQKKGISRKKTVVKIEKMSVTSETPKGTKGKKSGGAGGKDGGKRAMTLSVTQDSENPDLPEFVHPLMDHQAEHTEGKVFIPGCMTLVNLNLSRNKIGKQGMEYLLSMIQYQANRHRILAATPTAPGLLKLHIQRNAVSEQHPTYVKIQETMVTRDPTYKPEAKTPEDDIQSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>H2XV16</accession>
    <name>H2XV16_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">E2F/DP family winged-helix DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">wild type</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the E2F/DP family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002293">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XV16"/>
    <dbReference type="FunCoup" id="H2XV16">
        <property type="interactions" value="621"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000033500"/>
    <dbReference type="Ensembl" id="ENSCINT00000031528.1">
        <property type="protein sequence ID" value="ENSCINP00000033500.1"/>
        <property type="gene ID" value="ENSCING00000024912.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000169012"/>
    <dbReference type="HOGENOM" id="CLU_032091_2_0_1"/>
    <dbReference type="InParanoid" id="H2XV16"/>
    <dbReference type="OMA" id="GHEKSLG"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090575">
        <property type="term" value="C:RNA polymerase II transcription regulator complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14660">
        <property type="entry name" value="E2F_DD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000008">
        <property type="entry name" value="E2F transcription factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015633">
        <property type="entry name" value="E2F"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037241">
        <property type="entry name" value="E2F-DP_heterodim"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032198">
        <property type="entry name" value="E2F_CC-MB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003316">
        <property type="entry name" value="E2F_WHTH_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12081">
        <property type="entry name" value="TRANSCRIPTION FACTOR E2F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12081:SF18">
        <property type="entry name" value="TRANSCRIPTION FACTOR E2F2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16421">
        <property type="entry name" value="E2F_CC-MB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02319">
        <property type="entry name" value="WHD_E2F_TDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01372">
        <property type="entry name" value="E2F_TDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144074">
        <property type="entry name" value="E2F-DP heterodimerization region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="E2F/DP family winged-helix DNA-binding" evidence="6">
        <location>
            <begin position="9"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000033500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="269" mass="29710" checksum="9F506B886C9B1362" modified="2012-04-18" version="1">MADLASTSRHEKSLGLLTTKFVHLLKNAQNGVLDLKMAADELAVRQKRRIYDITNVLEGIGLIEKRSKNSIQWKGAGPECNDGDIAEKINILRQEIQSLEQTELDLDRQKTGVHQSLRNVTDDVDNNKLAYVTHEDLCRCFPGDTLLAIQAPSGTQLEVPIPEMADPNKRYQVHLKSTSGPVSVLLVNKDTSSPAVVVPVPPPPQAETAKPTELPLPSMQTDTNFLDDFPCSDTFEPLLSLSPLPSDQDYYFNLDMSEGACDLFDISLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>H2XV31</accession>
    <accession>A0A1W2W647</accession>
    <name>H2XV31_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein mL54</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100185381</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">wild type</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the mitochondrion-specific ribosomal protein mL54 family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01000459">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002125406.1">
        <property type="nucleotide sequence ID" value="XM_002125370.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XV31"/>
    <dbReference type="FunCoup" id="H2XV31">
        <property type="interactions" value="35"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000033515"/>
    <dbReference type="Ensembl" id="ENSCINT00000031127.1">
        <property type="protein sequence ID" value="ENSCINP00000033515.1"/>
        <property type="gene ID" value="ENSCING00000019896.1"/>
    </dbReference>
    <dbReference type="GeneID" id="100185381"/>
    <dbReference type="KEGG" id="cin:100185381"/>
    <dbReference type="GeneTree" id="ENSGT00390000001201"/>
    <dbReference type="HOGENOM" id="CLU_143073_0_0_1"/>
    <dbReference type="InParanoid" id="H2XV31"/>
    <dbReference type="OMA" id="WLFEMNV"/>
    <dbReference type="OrthoDB" id="10252718at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
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    <reference evidence="13" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2006" name="Dev. Biol." volume="292" first="546" last="554">
            <title>Systematic analysis of embryonic expression profiles of zinc finger genes in Ciona intestinalis.</title>
            <authorList>
                <person name="Miwata K."/>
                <person name="Chiba T."/>
                <person name="Horii R."/>
                <person name="Yamada L."/>
                <person name="Kubo A."/>
                <person name="Miyamura D."/>
                <person name="Satoh N."/>
                <person name="Satou Y."/>
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            <dbReference type="PubMed" id="16519883"/>
            <dbReference type="DOI" id="10.1016/j.ydbio.2006.01.024"/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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    <reference evidence="12" key="3">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GLI C2H2-type zinc-finger protein family.</text>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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    <reference evidence="2" key="2">
        <citation type="journal article" date="2003" name="Dev. Genes Evol." volume="213" first="211" last="212">
            <title>Genomewide surveys of developmentally relevant genes in Ciona intestinalis.</title>
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                <person name="Satou Y."/>
                <person name="Satoh N."/>
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            <dbReference type="DOI" id="10.1007/s00427-003-0330-z"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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    <reference evidence="2" key="3">
        <citation type="journal article" date="2004" name="Development" volume="131" first="4047" last="4058">
            <title>Gene expression profiles of transcription factors and signaling molecules in the ascidian embryo: towards a comprehensive understanding of gene networks.</title>
            <authorList>
                <person name="Imai K.S."/>
                <person name="Hino K."/>
                <person name="Yagi K."/>
                <person name="Satoh N."/>
                <person name="Satou Y."/>
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            <dbReference type="DOI" id="10.1242/dev.01270"/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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    <reference evidence="2" key="4">
        <citation type="submission" date="2005-04" db="EMBL/GenBank/DDBJ databases">
            <title>Expressed genes in Ciona intestinalis.</title>
            <authorList>
                <person name="Satou Y."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="3" key="5">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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            <strain evidence="3">wild type</strain>
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    <reference evidence="3" key="6">
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                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <source>
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    </evidence>
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    <accession>F6VI10</accession>
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        <name type="scientific">Ciona intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Phlebobranchia</taxon>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">wild type</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="ENSCING00000007998.3"/>
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        <property type="project" value="GOC"/>
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        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
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        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Rgp1"/>
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        <property type="entry name" value="REDUCED GROWTH PHENOTYPE 1 RGP1, YEAST -RELATED"/>
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        <property type="entry name" value="Rgp1"/>
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            <dbReference type="Ensembl" id="ENSCINP00000016376.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="386" mass="42996" checksum="04C2BCEF49E9CD03" modified="2017-06-07" version="1">MIEVVAKLRRGSVYFAGETIHCSLTFTNVPDTEPGNNTESETIAWASVQLHCFCSISEARVVLPPALMKNMLAKRKDVGPSTSFVPYKDEQGHCVLQTPVKILFCDLKLAPGESKVFKFQEAIPSDTPPTYRGPSCKYSYKLTVGTQRVGAPIKLLRIPVRVLVVYGLTEYQINEDQIPSNPFIEEKKTKGQLLDIATDVLSTITSRRSYKNYKIANSNGLMGVFSLMKTSAKIGEDIVGVFNFADGTVPCLQYMVCLQSEEILAEECQHSPSQGNAIISYAKHIEFCLFANQTHVILPIPLHVTPNFLTELVCLKWRLHFEFVTATSPLKGLDLETGQQRDRMWQGPGKIKTEVTSWDIPVQVLPTMPTQAENVAASHTLPLLRF</sequence>
</entry>
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    <accession>A0A1W5BJW2</accession>
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    <name>A0A1W5BJW2_CIOIN</name>
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        <recommendedName>
            <fullName evidence="9">RNA polymerase II subunit A C-terminal domain phosphatase SSU72</fullName>
            <shortName evidence="9">CTD phosphatase SSU72</shortName>
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        <name evidence="10" type="primary">LOC100187321</name>
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        <name type="scientific">Ciona intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
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            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
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                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">wild type</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Protein phosphatase that catalyzes the dephosphorylation of the C-terminal domain of RNA polymerase II. Plays a role in RNA processing and termination.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the SSU72 phosphatase family.</text>
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    <dbReference type="EC" id="3.1.3.16" evidence="9"/>
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        <property type="component" value="Chromosome 1"/>
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    <dbReference type="GO" id="GO:0008420">
        <property type="term" value="F:RNA polymerase II CTD heptapeptide repeat phosphatase activity"/>
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    <dbReference type="GO" id="GO:0031124">
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        <property type="project" value="UniProtKB-ARBA"/>
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    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000039">
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="3 9" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 9" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU369031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000009069.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="192" mass="22329" checksum="66E45E1C97DD0D28" modified="2017-06-07" version="1">MPKLSFAVVCSSNMNRSMEAHRCMSKRGYAVESFGSGNQVKLPGSSADRPNVYSFSTKYDDILTDLKQKDRNLYTQNGVLNMLDRNRRIKDRPQRFQDYVGQFDVIITLEERVYDQIMEDFNTRESETMEAVHVINLDIQDNHEEATIGAFVVCELSKKLEGSDDLDNDIDELLQDFEERSKRRIIHSVHFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>F6PU48</accession>
    <name>F6PU48_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
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    </protein>
    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
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            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01002998">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6PU48"/>
    <dbReference type="Ensembl" id="ENSCINT00000024114.1">
        <property type="protein sequence ID" value="ENSCINP00000023868.1"/>
        <property type="gene ID" value="ENSCING00000012890.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000067290"/>
    <dbReference type="HOGENOM" id="CLU_1639168_0_0_1"/>
    <dbReference type="InParanoid" id="F6PU48"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="35"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="101"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000023868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="162" mass="18332" checksum="8D4693BA4C8FD726" modified="2011-07-27" version="1">MATPSQRTFRPSYRNPNGDIQFENKTASAARSRDQLITCMLILSLSLAALSSSFTIHLAIENYKRMLENKELLMKIDLLNKEVFNPEKLTAANAINDTSTASWHSVKETPKQTTDSLSDAEVTNGTKAKFKCCDNAQTKTTLYPHRYYVAKFREIGDSLREF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>F6Q662</accession>
    <name>F6Q662_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">wild type</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the actin family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001884">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6Q662"/>
    <dbReference type="STRING" id="7719.ENSCINP00000018172"/>
    <dbReference type="Ensembl" id="ENSCINT00000018172.3">
        <property type="protein sequence ID" value="ENSCINP00000018172.3"/>
        <property type="gene ID" value="ENSCING00000008948.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156048"/>
    <dbReference type="HOGENOM" id="CLU_027965_0_2_1"/>
    <dbReference type="InParanoid" id="F6Q662"/>
    <dbReference type="OMA" id="KWIAKTE"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd10224">
        <property type="entry name" value="ASKHA_NBD_actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000131">
        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000291">
        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000047">
        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000058">
        <property type="entry name" value="Putative actin-related protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020902">
        <property type="entry name" value="Actin/actin-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004001">
        <property type="entry name" value="Actin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00190">
        <property type="entry name" value="ACTIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00406">
        <property type="entry name" value="ACTINS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00432">
        <property type="entry name" value="ACTINS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01132">
        <property type="entry name" value="ACTINS_ACT_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000018172.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="370" mass="41100" checksum="A1F5689632AFA85B" modified="2012-04-18" version="2">MSDSEKIKPSGVRNGSGLVKSGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIIKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHNVPIYEGYALPHAIARLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFEQEMATAASSTSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWITKQEYDEAGPSIVHRKCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>F6QHE0</accession>
    <name>F6QHE0_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Suppressor of cytokine signaling</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">socs7</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">wild type</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001766">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6QHE0"/>
    <dbReference type="STRING" id="7719.ENSCINP00000020168"/>
    <dbReference type="Ensembl" id="ENSCINT00000020168.3">
        <property type="protein sequence ID" value="ENSCINP00000020168.3"/>
        <property type="gene ID" value="ENSCING00000010048.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156314"/>
    <dbReference type="HOGENOM" id="CLU_535222_0_0_1"/>
    <dbReference type="InParanoid" id="F6QHE0"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035591">
        <property type="term" value="F:signaling adaptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008286">
        <property type="term" value="P:insulin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009968">
        <property type="term" value="P:negative regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd10388">
        <property type="entry name" value="SH2_SOCS7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035866">
        <property type="entry name" value="SOCS7_SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001496">
        <property type="entry name" value="SOCS_box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036036">
        <property type="entry name" value="SOCS_box-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10155">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 3-KINASE REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10155:SF5">
        <property type="entry name" value="SUPPRESSOR OF CYTOKINE SIGNALING 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07525">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00253">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00969">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158235">
        <property type="entry name" value="SOCS box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50225">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0341">Growth regulation</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="3" id="KW-0734">Signal transduction inhibitor</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="SH2" evidence="8">
        <location>
            <begin position="333"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="domain" description="SOCS box" evidence="9">
        <location>
            <begin position="447"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="13"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000020168.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="509" mass="58006" checksum="DE34EFD16849AF6F" modified="2011-07-27" version="1">MNQFATSAGPGARRKEFQHTRRYGKASEEKKNSEHESELRVPRMNVRRANERNYNGVSCNSDENVYGEIDVNPIVSVSFPTKSVVQAAKENDALLNFNLKISTNDIKNHTESAIDHSGFHPCTTSRLESDPEIGSNFFCHDSIPAQNNNFNDMLLLEQPKFKVRPRRHVYHTIELPDRNDISDGTPSNNHKVKIISSPPIPPKPHYLRSGFNSKLPPKLPPKVVNANAAMKRKQLNEEYFENIRKSMDFNLSESDEAGLQEEAISITIPGTETHPYWTISDTTTNTTQNLTTETSSTQLETPLIKNTPLTLDVHKQRRQEFVDSLKALKQCGWYWGNMSWEDAESMLAQRPGEEGVFLVRDSQDPLHILTLTIRSVELSIHHVRVEHTEGKFQLYDPDRGVSRGAANCVRHPNIVTFIKLAMKHSRSGTFIYFIKTRNMGVPPVQIRLLTPISRFTCVKSLKYYVRFTIRDSVPYDSITNLPIPHDLKEYLLNSPYFDATEDLQCALLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>F6QIR4</accession>
    <name>F6QIR4_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">protein kinase C</fullName>
            <ecNumber evidence="4">2.7.11.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">LOC100175164</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.13"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.13" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="F6QIR4"/>
    <dbReference type="FunCoup" id="F6QIR4">
        <property type="interactions" value="94"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000018412"/>
    <dbReference type="Ensembl" id="ENSCINT00000018412.3">
        <property type="protein sequence ID" value="ENSCINP00000018412.3"/>
        <property type="gene ID" value="ENSCING00000009071.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000168432"/>
    <dbReference type="InParanoid" id="F6QIR4"/>
    <dbReference type="OMA" id="ECIPEIA"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004697">
        <property type="term" value="F:diacylglycerol-dependent serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd11622">
        <property type="entry name" value="HR1_PKN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11623">
        <property type="entry name" value="HR1_PKN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05589">
        <property type="entry name" value="STKc_PKN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.160:FF:000002">
        <property type="entry name" value="Putative serine/threonine-protein kinase N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.160:FF:000003">
        <property type="entry name" value="Putative serine/threonine-protein kinase N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000058">
        <property type="entry name" value="Putative serine/threonine-protein kinase N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000038">
        <property type="entry name" value="serine/threonine-protein kinase N2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.160">
        <property type="entry name" value="HR1 repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011072">
        <property type="entry name" value="HR1_rho-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036274">
        <property type="entry name" value="HR1_rpt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037313">
        <property type="entry name" value="PKN_HR1_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02185">
        <property type="entry name" value="HR1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00742">
        <property type="entry name" value="Hr1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46585">
        <property type="entry name" value="HR1 repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51860">
        <property type="entry name" value="REM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13 17 19" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="REM-1" evidence="23">
        <location>
            <begin position="12"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="REM-1" evidence="23">
        <location>
            <begin position="98"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="21">
        <location>
            <begin position="615"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="22">
        <location>
            <begin position="875"/>
            <end position="943"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="511"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="908"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="19">
        <location>
            <begin position="56"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="565"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="644"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000018412.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="943" mass="107763" checksum="245F1CD615CBACC8" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>F6R2I4</accession>
    <accession>A0A1W2WIV2</accession>
    <name>F6R2I4_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tyrosinase copper-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">wild type</strain>
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    <reference evidence="7" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Melanosome membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tyrosinase family.</text>
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    <dbReference type="EMBL" id="EAAA01000472">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="F6R2I4"/>
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    <dbReference type="Ensembl" id="ENSCINT00000018374.3">
        <property type="protein sequence ID" value="ENSCINP00000018374.3"/>
        <property type="gene ID" value="ENSCING00000009054.3"/>
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    <dbReference type="GeneID" id="100179878"/>
    <dbReference type="KEGG" id="cin:100179878"/>
    <dbReference type="GeneTree" id="ENSGT00940000155336"/>
    <dbReference type="HOGENOM" id="CLU_053703_0_0_1"/>
    <dbReference type="InParanoid" id="F6R2I4"/>
    <dbReference type="OMA" id="FAYKERY"/>
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    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 10"/>
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        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:tyrosinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="P:melanin biosynthetic process"/>
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        <property type="entry name" value="hemocyanin A-type, units Ode to Odg-like"/>
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        <property type="entry name" value="Di-copper center containing domain from catechol oxidase"/>
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        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Di-copper_centre_dom_sf"/>
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        <property type="entry name" value="TSP1_rpt"/>
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    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050316">
        <property type="entry name" value="Tyrosinase/Hemocyanin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002227">
        <property type="entry name" value="Tyrosinase_Cu-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11474">
        <property type="entry name" value="TYROSINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11474:SF126">
        <property type="entry name" value="TYROSINASE-LIKE PROTEIN TYR-1-RELATED"/>
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        <property type="entry name" value="TSP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TYROSINASE"/>
    </dbReference>
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        <property type="entry name" value="Di-copper centre-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00497">
        <property type="entry name" value="TYROSINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0186">Copper</keyword>
    <keyword evidence="5" id="KW-0470">Melanin biosynthesis</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosinase copper-binding" evidence="6">
        <location>
            <begin position="119"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009928"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000018374.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="422" mass="49856" checksum="014168A6D4D5A05D" modified="2012-04-18" version="2">MFGDWSTWSSCIVSEWGGVQHRGRLCKTKLGRSANNRCVGAGSQSRSCSKVTRDFRIRKNLLDLTDEELHDFIQGFTKFKQDHSLFGYLNIAKWHGWPNRCPWYKHRIEEHKNGHCSWHNHPMFLPWHRLIMVQLELGLSRHMKNKTLGIPYWDWTDPTYKGIPDLVKNPTIYDPILKEYVPNPFYRTYVPSHPLVNNQTLYNYRAVERAGFLRHNLMLKNIIQAVNMPNYEFFDMTEIDAHGQVHNCMCVSKGGNCTYSMLKTAYSCFDSAFFLHHSQIDRVYALYQKLRHVLGTQDWTKESFLDAYKREHFFDFFNRPDVSGSWDWPMSPFCNASMNPSYVTLNENSWTVANSYYYQELFGYKYDSFDFARRDWKLLLKDLKQSYESKYYGKVVPYFSHLGKDLGEISHQSIFFTGDCTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F6R4L9</accession>
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    <gene>
        <name evidence="1" type="primary">LOC100183850</name>
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    <organism evidence="1 2">
        <name type="scientific">Ciona intestinalis</name>
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        <lineage>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">wild type</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01001986">
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_002130603.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6R4L9"/>
    <dbReference type="Ensembl" id="ENSCINT00000020087.3">
        <property type="protein sequence ID" value="ENSCINP00000020087.3"/>
        <property type="gene ID" value="ENSCING00000009967.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100183850"/>
    <dbReference type="KEGG" id="cin:100183850"/>
    <dbReference type="HOGENOM" id="CLU_1414708_0_0_1"/>
    <dbReference type="InParanoid" id="F6R4L9"/>
    <dbReference type="OMA" id="VYQHRLK"/>
    <dbReference type="OrthoDB" id="10146684at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000020087.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="192" mass="22250" checksum="83F58244B2848890" modified="2011-07-27" version="1">MALNRPRIDTAAVTKTSVVLGHHREPKTTTYNEVFELGRAFKSAPKPKRYELSCVAEIFHKTEKQYPRSIAQASFGKQSPKTTCTVYQHRLKALKTIMGIHSINSVPIGPQPSNYERENVTDTSQMKTDYVDRHHILRRWVDIQTSNIPTLPRINQQSEPWYRGQTEAAHQFKWPSRDADAIYQVRFNKNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>F6S034</accession>
    <name>F6S034_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glutaminase</fullName>
            <ecNumber evidence="3">3.5.1.2</ecNumber>
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    </protein>
    <organism evidence="6 7">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
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            <taxon>Ciona</taxon>
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    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-glutamine + H2O = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="3.5.1.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glutaminase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.2" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="F6S034"/>
    <dbReference type="STRING" id="7719.ENSCINP00000022393"/>
    <dbReference type="Ensembl" id="ENSCINT00000022639.2">
        <property type="protein sequence ID" value="ENSCINP00000022393.2"/>
        <property type="gene ID" value="ENSCING00000011807.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000010463"/>
    <dbReference type="HOGENOM" id="CLU_1405407_0_0_1"/>
    <dbReference type="InParanoid" id="F6S034"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="entry name" value="DD-peptidase/beta-lactamase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015868">
        <property type="entry name" value="Glutaminase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12544">
        <property type="entry name" value="GLUTAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12544:SF29">
        <property type="entry name" value="GLUTAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04960">
        <property type="entry name" value="Glutaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="beta-lactamase/transpeptidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000022393.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="194" mass="21446" checksum="E0E136A482D17132" modified="2012-04-18" version="2">MMRRRKSHHTVSLALCTVDGQRLSFGDKETPHPLNACATMFNYCLAHAQNGPEVMLHYLGKEPKPEDKGELTFNSDGKVWNPLTKSGAFMTSCLVFREMAVEERLDALHAFYDTLSGHEPLCCDNLSYNFKRSYAHEEIGAAYNLSSTQRLPRGTSEFIAEALDFHFQSSSTAMTSDACAVSAATLANNGICPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>F6S5Q9</accession>
    <name>F6S5Q9_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">wild type</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="EAAA01000049">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6S5Q9"/>
    <dbReference type="Ensembl" id="ENSCINT00000023555.2">
        <property type="protein sequence ID" value="ENSCINP00000023309.2"/>
        <property type="gene ID" value="ENSCING00000012506.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234625"/>
    <dbReference type="HOGENOM" id="CLU_1202183_0_0_1"/>
    <dbReference type="InParanoid" id="F6S5Q9"/>
    <dbReference type="OMA" id="NIANIIC"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049680">
        <property type="entry name" value="FLVCR1-2_SLC49-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10924">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10924:SF27">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 49 MEMBER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="31"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="73"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="161"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="211"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="35"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000023309.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="231" mass="26064" checksum="06B11F37DD87B2EE" modified="2012-04-18" version="2">MDKNQQERNEINSDLEPLINKKSQELKLYWYRWYILLQFALFSLLQTWASSMWSPIVKSVEASLNFTQHDFDLLADWGVIGVMCFIPVNMWMLVKYGMRVAVLSGMFCVFLGCGIKCLPLPTDQLKYAIHAGQILNGIGGSPVLSAPTYISNTWFPASERVFATGAMMMLGNLGGTTGYMLGPYLVEQPNNSSGLNGTSNVEAMVKEIKTYLYINFGFVFVVFFMMLTFFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>F6SH91</accession>
    <name>F6SH91_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SH3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="F6SH91"/>
    <dbReference type="Ensembl" id="ENSCINT00000024401.2">
        <property type="protein sequence ID" value="ENSCINP00000024155.2"/>
        <property type="gene ID" value="ENSCING00000013087.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183088"/>
    <dbReference type="HOGENOM" id="CLU_073617_1_0_1"/>
    <dbReference type="InParanoid" id="F6SH91"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050384">
        <property type="entry name" value="Endophilin_SH3RF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
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        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14167:SF116">
        <property type="entry name" value="CAP, ISOFORM AC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14167">
        <property type="entry name" value="SH3 DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00499">
        <property type="entry name" value="P67PHOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="3">
        <location>
            <begin position="11"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="3">
        <location>
            <begin position="77"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="3">
        <location>
            <begin position="145"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000024155.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="206" mass="23234" checksum="DD4B537CE39A3B26" modified="2011-07-27" version="1">MMSSDMPEPSTNQSTAKVLFNFTPGVPAELELLAGDFVEVEKIVDDFWYFGTNLKDGTQGQFPKDFVTFIQLPTTKPSEKLLFALDSFFPETSEDLPFEKGDIIVMESKIDESWVMGRCNNQRGMFPLNYTMEFRKSTKLKSRNEVLSQVQATMSLTAQLDNELSFKAGEIIDVCKYVDDAWAEGRIGDRTGVFPLSFTTKISINS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>F6SJ97</accession>
    <name>F6SJ97_CIOIN</name>
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        <recommendedName>
            <fullName evidence="1">Methyltransferase domain-containing protein</fullName>
        </recommendedName>
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    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01002242">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6SJ97"/>
    <dbReference type="Ensembl" id="ENSCINT00000027981.2">
        <property type="protein sequence ID" value="ENSCINP00000027735.2"/>
        <property type="gene ID" value="ENSCING00000015787.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165123"/>
    <dbReference type="HOGENOM" id="CLU_072082_1_0_1"/>
    <dbReference type="InParanoid" id="F6SJ97"/>
    <dbReference type="OMA" id="AILWETM"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025714">
        <property type="entry name" value="Methyltranfer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026913">
        <property type="entry name" value="METTL24"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32026">
        <property type="entry name" value="METHYLTRANSFERASE-LIKE PROTEIN 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32026:SF10">
        <property type="entry name" value="METHYLTRANSFERASE-LIKE PROTEIN 24-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13383">
        <property type="entry name" value="Methyltransf_22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransferase" evidence="1">
        <location>
            <begin position="3"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000027735.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="239" mass="27298" checksum="014540565352509B" modified="2012-04-18" version="2">MQYNCSHGEYLGGNPGNGDGAYWLCSEPRFWPRETDKEYKCLMYSFGVGGDFTFDDEIANRGCEVHSFDPTEQLEDGLVRESGVTFHKIGISATDTDKDGNGWKMRTLKTLIKELGHNGRYIDYLKMDTESYEEGLMQQVLDNDLAKCVRHYAQEIHLFGPISSPGKLAKCRHLYRAMTQLNDHGWRLYNTTDNCRYLKSPDPHARQLQVKPSMVNSGNAILWETMFVNFAATEPCKVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>F6SJF0</accession>
    <name>F6SJF0_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">tRNA-dihydrouridine synthase</fullName>
            <ecNumber evidence="9">1.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100186307</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">wild type</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9 11">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the dus family.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.-" evidence="9"/>
    <dbReference type="EMBL" id="EAAA01000908">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6SJF0"/>
    <dbReference type="FunCoup" id="F6SJF0">
        <property type="interactions" value="442"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000003786"/>
    <dbReference type="Ensembl" id="ENSCINT00000003786.3">
        <property type="protein sequence ID" value="ENSCINP00000003786.3"/>
        <property type="gene ID" value="ENSCING00000001869.3"/>
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    <dbReference type="GeneTree" id="ENSGT00550000074907"/>
    <dbReference type="HOGENOM" id="CLU_013299_4_2_1"/>
    <dbReference type="InParanoid" id="F6SJF0"/>
    <dbReference type="OMA" id="QRPHHDI"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017150">
        <property type="term" value="F:tRNA dihydrouridine synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102266">
        <property type="term" value="F:tRNA-dihydrouridine20a synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102267">
        <property type="term" value="F:tRNA-dihydrouridine20b synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02801">
        <property type="entry name" value="DUS_like_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000159">
        <property type="entry name" value="tRNA-dihydrouridine synthase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
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        <property type="entry name" value="DUS-like_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001269">
        <property type="entry name" value="DUS_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018517">
        <property type="entry name" value="tRNA_hU_synthase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11082">
        <property type="entry name" value="TRNA-DIHYDROURIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <ligand>
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        <source>
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        <source>
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    </evidence>
    <sequence length="309" mass="34762" checksum="09A266D6DE4409BA" modified="2012-04-18" version="2">LIKKMTNAVMELLTDPKPTKICAPMVRYSKHAFRLLVRKYDCDIAYTPMIVAKSFLESDSCRQNEFITSKDDTPLIVQFAAKNAKEFSEASELIYPFASGVGLNCGCPQRWAMAEGYGAKLLQKPEEIVDFIRFTKALVSDEKFTISVKIRLHEELARTVEMCRGLEKAGASFVSVHGRTSKERHQPVHYDAIKILKESVSIPIVANGGVNTYKDFDKVHKMTGANGVMVAQGLLNNPALFQGYKTTPLSCVKDWLDISLSYGTPFTPFHNHLIYMLDNVTSRAEKRIFNNLQSTPAVLEFLNKYFVDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>F6SPI0</accession>
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            <fullName evidence="4">Uncharacterized protein</fullName>
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        <name type="scientific">Ciona intestinalis</name>
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    <reference evidence="5" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">wild type</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="match status" value="2"/>
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        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="2"/>
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        <property type="match status" value="5"/>
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    </feature>
    <feature type="repeat" description="ANK" evidence="3">
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    </feature>
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    </evidence>
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        <source>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    </evidence>
    <sequence length="264" mass="29325" checksum="ECF96C8A8FCA73DD" modified="2012-04-18" version="2">MRLVQDIFHVSKLGLLIPVQDIIDRDGIGVLSQYDKDGHSPIHWACLGGHNHVVRYFAQIKAPLDIVANNQLGAQPIHWACVNGHVSTVDILLQAGISIDSVDNKGCTPLIIASQYGRTMLAGFLMGKGARLQITDKEGDNALMWAAFKGKCELTRLLIYSGFNPKQKDNFGQTSLHLASLSGDLLTVQLLCEQDGVDINLEDHNGNTPLKLAKGRQFSLDLLEKLKMLLCFSLLQLVCMDIRVIYLKFCLTQWNFEPCTYFLG</sequence>
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<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>F6TDF7</accession>
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        <recommendedName>
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    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
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                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
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                <person name="Meinertzhagen I.A."/>
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                <person name="Putnam N."/>
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                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
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                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
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                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
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                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
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                <person name="Miura S."/>
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                <person name="Nomoto H."/>
                <person name="Ohta F."/>
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                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
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                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <dbReference type="STRING" id="7719.ENSCINP00000001697"/>
    <dbReference type="Ensembl" id="ENSCINT00000001697.3">
        <property type="protein sequence ID" value="ENSCINP00000001697.3"/>
        <property type="gene ID" value="ENSCING00000000939.3"/>
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    <dbReference type="InParanoid" id="F6TDF7"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19756">
        <property type="entry name" value="Bbox2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR050143">
        <property type="entry name" value="TRIM/RBCC"/>
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    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
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        <property type="entry name" value="Znf_B-box"/>
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    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
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    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
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    <dbReference type="PANTHER" id="PTHR24103">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
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        <property type="entry name" value="ZF_RING_1"/>
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        <property type="entry name" value="ZF_RING_2"/>
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    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
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    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="62"/>
            <end position="109"/>
        </location>
    </feature>
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        <location>
            <begin position="141"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="188"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000001697.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="248" mass="28665" checksum="EC051D4986952248" modified="2012-04-18" version="2">MTMRSENMYTTIRTNDFLSSQGRSSTISKGYRSISKAITDTTRGRTSRATEFFDELPKELQCPKCGDLFDRPVLLSCSHTLCMDCAEDAIEYAELELKQRRRFSCPICRSAIKYNATGIESLPRNINIEEAVARFKKEGKSHYPVCDQHAAFKMEMLMWCEECKVNICPLCAVGDEHKQHKITPMQVVEKFQRIKRRLKNTKDALNEEIDVLGESIVKQQKIVYDLNEKAKELRLKVIFSTQIFLLLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>F6TJA8</accession>
    <accession>A0A1W2W838</accession>
    <name>F6TJA8_CIOIN</name>
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        <submittedName>
            <fullName evidence="3">Coiled-coil domain-containing protein 134</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100179157</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01001519">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002126605.2">
        <property type="nucleotide sequence ID" value="XM_002126569.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6TJA8"/>
    <dbReference type="FunCoup" id="F6TJA8">
        <property type="interactions" value="42"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000021785"/>
    <dbReference type="Ensembl" id="ENSCINT00000022031.2">
        <property type="protein sequence ID" value="ENSCINP00000021785.2"/>
        <property type="gene ID" value="ENSCING00000011389.2"/>
    </dbReference>
    <dbReference type="GeneID" id="100179157"/>
    <dbReference type="KEGG" id="cin:100179157"/>
    <dbReference type="GeneTree" id="ENSGT00390000020164"/>
    <dbReference type="HOGENOM" id="CLU_099195_0_0_1"/>
    <dbReference type="InParanoid" id="F6TJA8"/>
    <dbReference type="OMA" id="GIGFCNQ"/>
    <dbReference type="OrthoDB" id="5854099at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026321">
        <property type="entry name" value="CC134"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14735">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 134"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14735:SF1">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 134"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15002">
        <property type="entry name" value="ERK-JNK_inhib"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014090155" evidence="2">
        <location>
            <begin position="21"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="198"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="203"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000021785.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="223" mass="25796" checksum="8FAD5FE139DD2C05" modified="2011-07-27" version="1">MGSRVLLAVCTLVLALYCHGENEDNSELRLYRKLFRVKRKEHIAIVQKLLLMDDIEKHAAFIQMSMETIVKTIQESQQVLKSENYTPGRTFPTDTSAKEALSLILENTALFTDLIVRFPKLGHYFFEQNKMSWLRVMAPAITTCSATGVYDGDHQLILHSLQQELGIGEKDPLYINPFLQESKQGRPLTAEEREQIYREEKREKKKQKLKKRTGPRLTNKSEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>F6U095</accession>
    <name>F6U095_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
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    </protein>
    <organism evidence="8 9">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
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    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in the cell surface expression of neuronal nicotinic receptors. Binds RNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the unc-50 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="F6U095"/>
    <dbReference type="FunCoup" id="F6U095">
        <property type="interactions" value="1078"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000025619"/>
    <dbReference type="Ensembl" id="ENSCINT00000025865.2">
        <property type="protein sequence ID" value="ENSCINP00000025619.2"/>
        <property type="gene ID" value="ENSCING00000002962.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000018553"/>
    <dbReference type="HOGENOM" id="CLU_066239_0_0_1"/>
    <dbReference type="InParanoid" id="F6U095"/>
    <dbReference type="OMA" id="ETAIWEM"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007881">
        <property type="entry name" value="UNC-50"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12841">
        <property type="entry name" value="PROTEIN UNC-50 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12841:SF6">
        <property type="entry name" value="PROTEIN UNC-50 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05216">
        <property type="entry name" value="UNC-50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="78"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="109"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="158"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="184"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00046103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000025619.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="223" mass="26130" checksum="6A650583EB9C61EC" modified="2012-04-18" version="2">LLETISYTVGEQHGRNKLCTTAGEKSYKYLRRLIRFRQMDFEFALWQMMHLFIAPQKVYRNFQYRKQTKNQFARDDPAFLVLLSLCFCVTSIGFGLVMKLSALKIFKLLLWVVLVDCIFVGVIIATAMWFLTNRFLVHISRVGQDIEWAYAFDVHLNAFFPVLMILHCLQLFLINLLALIDKEYFISTLVGNTFWLIALGYYIYITFLGYSGKSFINNDVSYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>F6UQ45</accession>
    <name>F6UQ45_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">BACK domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="F6UQ45"/>
    <dbReference type="Ensembl" id="ENSCINT00000028917.2">
        <property type="protein sequence ID" value="ENSCINP00000028671.2"/>
        <property type="gene ID" value="ENSCING00000020855.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165174"/>
    <dbReference type="HOGENOM" id="CLU_1380857_0_0_1"/>
    <dbReference type="InParanoid" id="F6UQ45"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632:SF3">
        <property type="entry name" value="KELCH-LIKE PROTEIN 32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632">
        <property type="entry name" value="LD33804P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000028671.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="198" mass="22295" checksum="6BFD1EEB60C281ED" modified="2012-04-18" version="2">MNWMKFDLASNESYLSELLKFVDFYEMSPKMLEDMVSVEPLIRNLRGTDCSFHDALVQRAKTTETSLISLGGNNSLTKVTKLDLQTKQWSSLPDLPVGRLSAAAVVINDVLYHLAGDLQIDGKVTNIVYRMKLKEKVLKWEKVASMNVERYVFGAAVINGVIFVLGGTDENNKSVSTGEYYVVPLNKWIQLKPMKVAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>F6VJS2</accession>
    <name>F6VJS2_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Voltage-dependent L-type calcium channel subunit beta-2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Calcium channel voltage-dependent subunit beta 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Beta subunit of voltage-dependent calcium channels which contributes to the function of the calcium channel by increasing peak calcium current. Plays a role in shifting voltage dependencies of activation and inactivation of the channel. May modulate G protein inhibition. May contribute to beta-adrenergic augmentation of Ca(2+) influx in cardiomyocytes, thereby regulating increases in heart rate and contractile force. Involved in membrane targeting of the alpha-1 subunit CACNA1C.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of a calcium channel complex consisting of a pore-forming alpha subunit (CACNA1S) and the ancillary subunits CACNB1 or CACNB2, CACNG1 and CACNA2D1. The channel complex contains alpha, beta, gamma and delta subunits in a 1:1:1:1 ratio, i.e. it contains either CACNB1 or CACNB2. Interacts with CACNA1C. Interacts with RRAD; interaction may be involved in beta-adrenergic regulation of heart rate and contractile force. Interaction with RRAD regulates the trafficking of CACNA1C to the cell membrane. Interacts with TMIGD2. Interacts with CAMK2D. Interacts with CBARP. Interacts with CAMK2A.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the calcium channel beta subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="F6VJS2"/>
    <dbReference type="FunCoup" id="F6VJS2">
        <property type="interactions" value="8"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000002872"/>
    <dbReference type="Ensembl" id="ENSCINT00000002872.3">
        <property type="protein sequence ID" value="ENSCINP00000002872.3"/>
        <property type="gene ID" value="ENSCING00000001461.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182837"/>
    <dbReference type="HOGENOM" id="CLU_021995_0_0_1"/>
    <dbReference type="InParanoid" id="F6VJS2"/>
    <dbReference type="OMA" id="DSNFSHH"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005891">
        <property type="term" value="C:voltage-gated calcium channel complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008331">
        <property type="term" value="F:high voltage-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006816">
        <property type="term" value="P:calcium ion transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd11863">
        <property type="entry name" value="SH3_CACNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000432">
        <property type="entry name" value="Voltage-dependent L-type calcium channel subunit beta-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046937">
        <property type="entry name" value="CAB1-4_N_A-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008145">
        <property type="entry name" value="GK/Ca_channel_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005444">
        <property type="entry name" value="VDCC_L_b2su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000584">
        <property type="entry name" value="VDCC_L_bsu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11824">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00625">
        <property type="entry name" value="Guanylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12052">
        <property type="entry name" value="VGCC_beta4Aa_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01626">
        <property type="entry name" value="LCACHANNELB"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01628">
        <property type="entry name" value="LCACHANNELB2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00072">
        <property type="entry name" value="GuKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="5" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="7" id="KW-0407">Ion channel</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <keyword evidence="7" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="domain" description="SH3" evidence="13">
        <location>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
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                <person name="Rash S."/>
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                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
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                <person name="Detter C."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Yamada L."/>
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                <person name="Wiley G.B."/>
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                <person name="Roe B.A."/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="7" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="7">wild type</strain>
        </source>
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    <reference evidence="7" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">mRNA cap-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation. In the eIF-3 complex, eif3d specifically recognizes and binds the 7-methylguanosine cap of a subset of mRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex.</text>
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        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="5">The RNA gate region regulates mRNA cap recognition to prevent promiscuous mRNA-binding before assembly of eif3d into the full eukaryotic translation initiation factor 3 (eIF-3) complex.</text>
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        <text evidence="5">Belongs to the eIF-3 subunit D family.</text>
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        <property type="component" value="Chromosome 4"/>
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        <property type="entry name" value="EIF-3_zeta"/>
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        <location>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
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            <fullName evidence="9">Cas1p 10 TM acyl transferase domain-containing protein</fullName>
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        <name type="scientific">Ciona intestinalis</name>
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            <taxon>Ciona</taxon>
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    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">wild type</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PC-esterase family. CASD1 subfamily.</text>
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    <dbReference type="EMBL" id="EAAA01000098">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="project" value="GO_Central"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="match status" value="1"/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
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                <person name="Doyle S."/>
                <person name="Francino P."/>
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                <person name="Kobayashi K."/>
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                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
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                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
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                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
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                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="PubMed" id="18854010"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Matsumoto J."/>
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                <person name="Wiley G.B."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="9">wild type</strain>
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        <text evidence="6">Component of the large ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. Binds to G-rich structures in 28S rRNA and in mRNAs. Plays a regulatory role in the translation apparatus; inhibits cell-free translation of mRNAs.</text>
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        <text evidence="1">Belongs to the universal ribosomal protein uL30 family.</text>
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    </dbReference>
    <dbReference type="FunFam" id="3.30.1390.20:FF:000003">
        <property type="entry name" value="60S ribosomal protein L7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1390.20">
        <property type="entry name" value="Ribosomal protein L30, ferredoxin-like fold domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036919">
        <property type="entry name" value="Ribo_uL30_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039699">
        <property type="entry name" value="Ribosomal_uL30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018038">
        <property type="entry name" value="Ribosomal_uL30_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005998">
        <property type="entry name" value="Ribosomal_uL30_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035808">
        <property type="entry name" value="Ribosomal_uL30_euk_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016082">
        <property type="entry name" value="Ribosomal_uL30_ferredoxin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012988">
        <property type="entry name" value="Ribosomal_uL30_N_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01310">
        <property type="entry name" value="uL30_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11524">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11524:SF16">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00327">
        <property type="entry name" value="Ribosomal_L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08079">
        <property type="entry name" value="Ribosomal_L30_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55129">
        <property type="entry name" value="Ribosomal protein L30p/L7e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00634">
        <property type="entry name" value="RIBOSOMAL_L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL30 N-terminal eukaryotes" evidence="8">
        <location>
            <begin position="12"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL30-like ferredoxin-like fold" evidence="7">
        <location>
            <begin position="88"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00040575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00041271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00045415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000014791.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="246" mass="28470" checksum="3073B25364529F8C" modified="2012-04-18" version="2">SNMVASKKLPRVPESLLKKRKEYQAQKAKQAQAAILNKKKNKEKRKIIFKRAEQYVKEYRIKERDGIRLARQARTHNNYYVPDEPKLAFVIRIRGINQVSPKVKKVLQLFRLRQIHNGVFIKLNKPTLQMLRIAEPYIAWGYPNLKTVRELIYKRGHGKVNKSRIPITDNNIIEDSLGKKGIICVEDLIHEIFTVGPNFKAAAAFLWPFKLSSPRGGFQKVTNHFVEGGDCGNREDKINALVRQMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>F6YJB7</accession>
    <accession>A0A1W5BEW1</accession>
    <name>F6YJB7_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="11">DNA repair protein complementing XP-A cells homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100179498</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the XPA family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_018670863.1">
        <property type="nucleotide sequence ID" value="XM_018815318.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6YJB7"/>
    <dbReference type="FunCoup" id="F6YJB7">
        <property type="interactions" value="478"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000003306"/>
    <dbReference type="Ensembl" id="ENSCINT00000003306.3">
        <property type="protein sequence ID" value="ENSCINP00000003306.3"/>
        <property type="gene ID" value="ENSCING00000001643.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100179498"/>
    <dbReference type="KEGG" id="cin:100179498"/>
    <dbReference type="GeneTree" id="ENSGT00390000002721"/>
    <dbReference type="HOGENOM" id="CLU_053731_1_0_1"/>
    <dbReference type="InParanoid" id="F6YJB7"/>
    <dbReference type="OMA" id="EFGEDTY"/>
    <dbReference type="OrthoDB" id="68328at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000110">
        <property type="term" value="C:nucleotide-excision repair factor 1 complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901255">
        <property type="term" value="P:nucleotide-excision repair involved in interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000715">
        <property type="term" value="P:nucleotide-excision repair, DNA damage recognition"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070914">
        <property type="term" value="P:UV-damage excision repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd21076">
        <property type="entry name" value="DBD_XPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.530.10:FF:000001">
        <property type="entry name" value="DNA repair protein complementing XP-A cells"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.530.10">
        <property type="entry name" value="XPA C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000465">
        <property type="entry name" value="XPA/RAD14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022656">
        <property type="entry name" value="XPA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037129">
        <property type="entry name" value="XPA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022652">
        <property type="entry name" value="Znf_XPA_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00598">
        <property type="entry name" value="rad14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10142">
        <property type="entry name" value="DNA REPAIR PROTEIN COMPLEMENTING XP-A CELLS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10142:SF0">
        <property type="entry name" value="DNA REPAIR PROTEIN COMPLEMENTING XP-A CELLS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05181">
        <property type="entry name" value="XPA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01286">
        <property type="entry name" value="XPA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="Putative DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="XPA C-terminal" evidence="10">
        <location>
            <begin position="141"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF05181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000003306.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="279" mass="31872" checksum="D829CF709847E23D" modified="2011-07-27" version="1">MSNDIEEKQPNVECLVDNDIDASPSKHVVTKTRAAQLARSERNRQKALLLRQSKLANQQINKKDGKTSVGCTSVVDSGAGFLIEENSQVEAKKMKVTEEPAPVIAGDLTICIECDKPFLDSYLSSTYDHPVCDKCKDTEDKHSVITKSEAKETYLLSEVDLMKREPLLKCVVRRNPRNNRWGDMKLYLKLQVEKRALEVWGSEEAIEDEHEVRRKKKEDAKIKKFNKNMKQLRKEVRSSLYSVKVQGHQHSYGEEQHIGGDEYNKTCSTCGHQVTFEKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>F6YTZ8</accession>
    <name>F6YTZ8_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="235"/>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    </evidence>
    <sequence length="268" mass="28712" checksum="8B22050AD6623D7C" modified="2012-04-18" version="2">NEWMTALASGAVAGFCVDVTLFPVDTVKTRLQSSQGFIKAGGFKRIYSGIGPAALGSAPGAAVFFTAYDTTRNLLKKPDRSFGHNLAVDMTAASVGEVVACLIRVPVEVIKQRSQVTKNANSFRIFLSCIRTEGVRGMYRGYRSTVVREIPFSLIQFPLWEFLKVTVAKYRGKESPDAIESAVCGFFSGGIAAAVTTPLDVAKTRIILAQTNDSAAAGNILPVLREVWSTGGARALFAGVLPRVVWISVGGFVFLGMYDTTKSLLNGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>F6Z4P5</accession>
    <name>F6Z4P5_CIOIN</name>
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        <recommendedName>
            <fullName evidence="3">C-type lectin domain-containing protein</fullName>
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    <organism evidence="4 5">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
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            <taxon>Phlebobranchia</taxon>
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            <taxon>Ciona</taxon>
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    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">wild type</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="F6Z4P5"/>
    <dbReference type="Ensembl" id="ENSCINT00000020987.3">
        <property type="protein sequence ID" value="ENSCINP00000020987.3"/>
        <property type="gene ID" value="ENSCING00000013197.2"/>
    </dbReference>
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        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="F:carbohydrate binding"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00037">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050111">
        <property type="entry name" value="C-type_lectin/snaclec_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22803">
        <property type="entry name" value="MANNOSE, PHOSPHOLIPASE, LECTIN RECEPTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003351913" description="C-type lectin domain-containing protein" evidence="2">
        <location>
            <begin position="19"/>
            <end position="207"/>
        </location>
    </feature>
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        <location>
            <begin position="169"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="3">
        <location>
            <begin position="23"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="207" mass="22779" checksum="CD7382566E825DF0" modified="2012-04-18" version="2">MKLLALILILQSLEVVLGSWYSLATTEYFVNASGKIVKSYKAAVEACALMNATLALVKTEQVHSFLVEKIGNVTSIPYSFYIGLKRSNVSTTGFQWNDGANETASNTLWLKGEPNNNYRIENCVQMGLREPRKIILYKWNDVFCNAPGRYICQRSGAITPTASKSPAPYYVIAIVGIIFVAAAGCALFYFKKKGNPASEKQVTNDAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>F6ZAE8</accession>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
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    <sequence length="404" mass="47168" checksum="34047B3F6C511700" modified="2012-04-18" version="2">MGNANGTDRKRRKWKDKKKICTKVRKESNQDDERSYHGDKYDRLKRALALEKDVHGKKPRLKKTKVRRVQNESVCSDESSAAKIRNRRKNNRPKRGKVNKEKNKQDKFHKPEAVGQNRASQFEREFKMGAKLSPSKRCIKWLCEQPSVSDWSKGATPSTVSVQCTAGTSKYVKLALPNKKRGENSEGKNVPAASGKRCKKDRMRKGKTRKRTAGSKICLRKNSCIFKKSAKKRKHKKKRKDVNVKTICKECVRNKWKSRLEQTRKKSKFIYEKKHRTGHYLSREKGSTSLGWAILQVIARLENHNKAYPLKILEQLKSKWKFTELNLVGLNSALEWMRKHKLLLCKVTRNRDDQFQVNKSNPSFEKRLKKLCKDDKQVPRLQFQSGIPHCVSINTQLKQKHHLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>F6ZRF8</accession>
    <name>F6ZRF8_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">wild type</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01001487">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6ZRF8"/>
    <dbReference type="Ensembl" id="ENSCINT00000028470.2">
        <property type="protein sequence ID" value="ENSCINP00000028224.2"/>
        <property type="gene ID" value="ENSCING00000016223.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161814"/>
    <dbReference type="HOGENOM" id="CLU_1239760_0_0_1"/>
    <dbReference type="InParanoid" id="F6ZRF8"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00037">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050801">
        <property type="entry name" value="Ca-Dep_Lectins_ImmuneDev"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22801:SF63">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22801">
        <property type="entry name" value="LITHOSTATHINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003352575" description="C-type lectin domain-containing protein" evidence="2">
        <location>
            <begin position="17"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="155"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="3">
        <location>
            <begin position="23"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000028224.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="223" mass="25443" checksum="CEBA5C6D9EF517B9" modified="2012-04-18" version="2">MKALLVLVSIVHLTFGHSCFSPEGNYDYYAMSQNRQQTLNIRQAENKCKSLDGCLAFSNLAEVERFLLMKNKSNRTAWYIGLTKTERGWTWSDGSLLNDNLSNWQINKQLTNIDNCVALTQWSNYKWIVRSCSRVINFVCQRAIVVETSILHEEAFYPVVGLCGLLALCCVGLLIMYKRKSTAGDRANLVNNEVAVVHRNVIYQAETIGNYEEVSPRYDEVCH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>F6ZTN4</accession>
    <name>F6ZTN4_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein Spindly-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100182525</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="F6ZTN4"/>
    <dbReference type="FunCoup" id="F6ZTN4">
        <property type="interactions" value="9"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000023089"/>
    <dbReference type="Ensembl" id="ENSCINT00000023335.2">
        <property type="protein sequence ID" value="ENSCINP00000023089.2"/>
        <property type="gene ID" value="ENSCING00000012346.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00510000047951"/>
    <dbReference type="HOGENOM" id="CLU_031713_1_0_1"/>
    <dbReference type="InParanoid" id="F6ZTN4"/>
    <dbReference type="OMA" id="NANECNQ"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000940">
        <property type="term" value="C:outer kinetochore"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043515">
        <property type="term" value="F:kinetochore binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000132">
        <property type="term" value="P:establishment of mitotic spindle orientation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007080">
        <property type="term" value="P:mitotic metaphase chromosome alignment"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034501">
        <property type="term" value="P:protein localization to kinetochore"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051149">
        <property type="entry name" value="Spindly/BICDR_Dynein_Adapter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32123">
        <property type="entry name" value="BICD FAMILY-LIKE CARGO ADAPTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32123:SF9">
        <property type="entry name" value="PROTEIN SPINDLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="2"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="101"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="317"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000023089.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="461" mass="54146" checksum="E48761B702214735" modified="2012-04-18" version="2">MANSFQNEIESLKETIEQNNKDLYNAGVQGKALLERNEQLENEIQQLHIEYTKTNEELEQEKYSLKSRIDVKKSEKRLENSILEEKQSLRIDVDRKCEGLDKLHRAEISAIKHQMKELQNELDESRLIQRQQEDKINKQNELLDDVEARRRQIHKYVIDSCTTELELATEVEKLKIEIIKLRKESMQHQNLNERYELQVAAMQKELFDLNEQKRNVEKDRETYYNQLERCRQEKRDVQSELDAVMFQASDPNSRGNSLFGEVEDKRVIAEKNLISAQIKLKCLKKRFDQQVSYALHLKTKIAQLWQTRGGSAEQDRIRRLEVTLSTTRNDYEKAMSKISKLEVQKSLYKQAEPNNKYHDTKYECHYDVTSIVTSQLFDKSTKVTSELKETKKELDETQLFLMAETNKVSEAERALYRSRCDCDTLRAKVVKLNLEIAQLKLEHGSGGRVKSAVKKGNREII</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>F6ZUU0</accession>
    <name>F6ZUU0_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">ATP-dependent RNA helicase</fullName>
            <ecNumber evidence="11">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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    <reference evidence="15" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
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        <text evidence="11">RNA helicase.</text>
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    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
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            <dbReference type="ChEBI" id="CHEBI:15377"/>
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        <text evidence="8">Interacts with 28S rRNA. Interacts with double-stranded RNA substrates in vitro; the interaction stimulates ATPase activity.</text>
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        <text evidence="11">The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.</text>
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        <text evidence="6">Belongs to the DEAD box helicase family. DDX55/SPB4 subfamily.</text>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
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                <person name="Huang W."/>
                <person name="Kawashima T."/>
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                <person name="Meinertzhagen I.A."/>
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                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
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                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Sasakura Y."/>
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                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
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                <person name="Wiley G.B."/>
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        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
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        <property type="entry name" value="Small_GTP-bd"/>
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        <property type="entry name" value="Transl_B-barrel_sf"/>
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        <property type="entry name" value="small_GTP"/>
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        <property type="entry name" value="RIBOSOME-RELEASING FACTOR 2, MITOCHONDRIAL"/>
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        <property type="entry name" value="Translation proteins"/>
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        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
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    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="5">
        <location>
            <begin position="27"/>
            <end position="315"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
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        <source>
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    <sequence length="736" mass="82083" checksum="4C22AB2F5DCF382F" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>F7AG43</accession>
    <accession>A0A1W2W1U1</accession>
    <name>F7AG43_CIOIN</name>
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        <submittedName>
            <fullName evidence="6">V-type proton ATPase subunit E-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100178177</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">wild type</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the V-ATPase E subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001313">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002121757.1">
        <property type="nucleotide sequence ID" value="XM_002121721.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F7AG43"/>
    <dbReference type="FunCoup" id="F7AG43">
        <property type="interactions" value="368"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000026021"/>
    <dbReference type="Ensembl" id="ENSCINT00000026267.2">
        <property type="protein sequence ID" value="ENSCINP00000026021.2"/>
        <property type="gene ID" value="ENSCING00000014372.2"/>
    </dbReference>
    <dbReference type="GeneID" id="100178177"/>
    <dbReference type="KEGG" id="cin:100178177"/>
    <dbReference type="GeneTree" id="ENSGT00390000002730"/>
    <dbReference type="HOGENOM" id="CLU_073641_2_0_1"/>
    <dbReference type="InParanoid" id="F7AG43"/>
    <dbReference type="OMA" id="QHMMAFI"/>
    <dbReference type="OrthoDB" id="10263003at2759"/>
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        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033178">
        <property type="term" value="C:proton-transporting two-sector ATPase complex, catalytic domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2320.30">
        <property type="entry name" value="ATP synthase, E subunit, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00311">
        <property type="entry name" value="ATP_synth_E_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038495">
        <property type="entry name" value="ATPase_E_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002842">
        <property type="entry name" value="ATPase_V1_Esu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45715">
        <property type="entry name" value="ATPASE H+-TRANSPORTING V1 SUBUNIT E1A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="vATP-synt_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160527">
        <property type="entry name" value="V-type ATPase subunit E-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="88"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00045737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000026021.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="226" mass="26024" checksum="DD813CED49DED65A" modified="2012-04-18" version="1">MGLSEVEVKKQIDHMIAFIDQEADEKVDEIMAKADEEFEIEKSRLVQQQRQKIMTYYERKQKQLEQQKKVQQSQLVNAARLKILKYREDHIQNILSEAKDQLSDLKRNQSQYNSLLLGLISQGLFQLLEDKVIVQCLKEDVQTVKALIPDVVDAFKKSTNRVAVVEVNESSFLTPDTCGGVIMTSANGAIRVRNTLDARLDLIGRQMLPEIREVLFGKNMNRKFLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>F7AKG2</accession>
    <name>F7AKG2_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">DNA topoisomerase 2</fullName>
            <ecNumber evidence="16">5.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">wild type</strain>
        </source>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks.</text>
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    <comment type="catalytic activity">
        <reaction evidence="1 22 23">
            <text>ATP-dependent breakage, passage and rejoining of double-stranded DNA.</text>
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        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
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    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="16">Homodimer.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 16">Belongs to the type II topoisomerase family.</text>
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        <property type="protein sequence ID" value="ENSCINP00000017040.3"/>
        <property type="gene ID" value="ENSCING00000008347.3"/>
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        <property type="term" value="P:resolution of meiotic recombination intermediates"/>
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    <feature type="region of interest" description="Disordered" evidence="17">
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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        <source>
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        <source>
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        <source>
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        <source>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>F7AUU8</accession>
    <name>F7AUU8_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Threonylcarbamoyl-AMP synthase</fullName>
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        </recommendedName>
    </protein>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">wild type</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Cytoplasmic and mitochondrial threonylcarbamoyl-AMP synthase required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Catalyzes the conversion of L-threonine, HCO(3)(-)/CO(2) and ATP to give threonylcarbamoyl-AMP (TC-AMP) as the acyladenylate intermediate, with the release of diphosphate. Participates in t(6)A37 formation in cytoplasmic and mitochondrial tRNAs. May regulate the activity of some transporters.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonine + hydrogencarbonate + ATP = L-threonylcarbamoyladenylate + diphosphate + H2O</text>
            <dbReference type="Rhea" id="RHEA:36407"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="ChEBI" id="CHEBI:73682"/>
            <dbReference type="EC" id="2.7.7.87"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="15">Interacts with RSC1A1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the SUA5 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.87" evidence="5"/>
    <dbReference type="EMBL" id="EAAA01000350">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F7AUU8"/>
    <dbReference type="FunCoup" id="F7AUU8">
        <property type="interactions" value="12"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000023355"/>
    <dbReference type="Ensembl" id="ENSCINT00000023601.2">
        <property type="protein sequence ID" value="ENSCINP00000023355.2"/>
        <property type="gene ID" value="ENSCING00000007564.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015364"/>
    <dbReference type="HOGENOM" id="CLU_031397_5_1_1"/>
    <dbReference type="InParanoid" id="F7AUU8"/>
    <dbReference type="OMA" id="WSNTEGI"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061710">
        <property type="term" value="F:L-threonylcarbamoyladenylate synthase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
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    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006450">
        <property type="term" value="P:regulation of translational fidelity"/>
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        <property type="project" value="GO_Central"/>
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    <dbReference type="FunFam" id="3.90.870.10:FF:000007">
        <property type="entry name" value="YrdC N6-threonylcarbamoyltransferase domain containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.870.10">
        <property type="entry name" value="DHBP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017945">
        <property type="entry name" value="DHBP_synth_RibB-like_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006070">
        <property type="entry name" value="Sua5-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050156">
        <property type="entry name" value="TC-AMP_synthase_SUA5"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00057">
        <property type="entry name" value="L-threonylcarbamoyladenylate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17490">
        <property type="entry name" value="SUA5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17490:SF10">
        <property type="entry name" value="THREONYLCARBAMOYL-AMP SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01300">
        <property type="entry name" value="Sua5_yciO_yrdC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55821">
        <property type="entry name" value="YrdC/RibB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51163">
        <property type="entry name" value="YRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="YrdC-like" evidence="16">
        <location>
            <begin position="1"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00015492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00058524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00063146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000023355.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="215" mass="23795" checksum="A1C07F9A6AE405E8" modified="2012-04-18" version="2">EDVIESCVRILHRGGIVALPTDTLYGVACLAQSTEAVEKLYEIKARNPLKPVAICVGDLYKVCQWTRFPQGLIHLLEDLLPGPVTIVTERSPSLNNTLNPKTSLVGIRVPDHKFIKDLTNRLREPLALTSANVSGEESSLRIEDFRSLWPKLDAVVDDGEVGTRYVNKTLQYAKEGSTVVQILNDGVSFKILRPGCAYENTVSVLKCKYGLKLTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>F7AXZ4</accession>
    <name>F7AXZ4_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rho-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01001502">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F7AXZ4"/>
    <dbReference type="FunCoup" id="F7AXZ4">
        <property type="interactions" value="12"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000007508"/>
    <dbReference type="Ensembl" id="ENSCINT00000007508.3">
        <property type="protein sequence ID" value="ENSCINP00000007508.3"/>
        <property type="gene ID" value="ENSCING00000003651.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000168361"/>
    <dbReference type="HOGENOM" id="CLU_023268_0_0_1"/>
    <dbReference type="InParanoid" id="F7AXZ4"/>
    <dbReference type="OMA" id="SCEMISE"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030833">
        <property type="term" value="P:regulation of actin filament polymerization"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051056">
        <property type="term" value="P:regulation of small GTPase mediated signal transduction"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057323">
        <property type="entry name" value="RHG40/28/18_ubiquitin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14963">
        <property type="entry name" value="RHO GTPASE ACTIVATING PROTEIN 18,19-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14963:SF1">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN CONUNDRUM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25442">
        <property type="entry name" value="Ubiquitin_RHG40_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rho-GAP" evidence="2">
        <location>
            <begin position="87"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000007508.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="443" mass="49990" checksum="660221F0BB5350F4" modified="2011-07-27" version="1">MSYSETADKQNLPNFSLVREPLGITKISDLSPTDMRKVQSLALIELTALFDLRGIELKRRKAKAKIKDAGVFGSPLTTLIEWDRKRTNLPTIKIPQVLREIIIFLEAKALKDEGILRKSGSAARIKLLKQDIENRFGPNPDTSAIAESSLWRDAHPHDVAALLKLFLRELPDPLLTTEYIDAFHSCEMISERKEQLQALNLLIILLHEMHRDSLQFLLSFLGLVVANEPYNKMGLNNVAMITAPNLFMARKQSKREASTTAASQDLKRAAGTSNIVRMLIKYHRLLWTVPAFMLTQVRHMHRAETSAKKHTTQSKDKYKVKFFQGKTKDGNKKSSSAKQPLEYEAVKGVIRVQAPDMLISSMAVQLDNTITASDIIARFQRQSERIRQTGSQTKPVVINNTTHALFEVGGNIHQRCLDPDTNMSALMRVNPDASWFIKPRTGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>F7B349</accession>
    <name>F7B349_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nucleotide exchange factor SIL1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Required for protein translocation and folding in the endoplasmic reticulum (ER). Functions as a nucleotide exchange factor for the ER lumenal chaperone HSPA5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SIL1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="F7B349"/>
    <dbReference type="FunCoup" id="F7B349">
        <property type="interactions" value="69"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000005312"/>
    <dbReference type="Ensembl" id="ENSCINT00000005313.3">
        <property type="protein sequence ID" value="ENSCINP00000005312.3"/>
        <property type="gene ID" value="ENSCING00000002608.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153909"/>
    <dbReference type="HOGENOM" id="CLU_046547_1_0_1"/>
    <dbReference type="InParanoid" id="F7B349"/>
    <dbReference type="OMA" id="FQPTHEW"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000774">
        <property type="term" value="F:adenyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000148">
        <property type="entry name" value="SIL1 nucleotide exchange factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050693">
        <property type="entry name" value="Hsp70_NEF-Inhibitors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013918">
        <property type="entry name" value="Nucleotide_exch_fac_Fes1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19316:SF35">
        <property type="entry name" value="NUCLEOTIDE EXCHANGE FACTOR SIL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19316">
        <property type="entry name" value="PROTEIN FOLDING REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08609">
        <property type="entry name" value="Fes1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0811">Translocation</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Nucleotide exchange factor Fes1" evidence="11">
        <location>
            <begin position="88"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000005312.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="394" mass="44367" checksum="2497AC0CD0DE179B" modified="2012-04-18" version="2">MAFVPTHEWQEVKEGQAIPPGLHVKMDMSGGGRWAKLMDEGGGGKNMQVMTVEQEDGKKEGSPQMDGHSVAELKQALKKIKAESTLDSKVSNHKTQEDVKKKFKSMDEIKEVLGQYEMKQESDVDVMKKLLGVLRTSGEESSIILALQDLEYYVHQIDNANDLVKIGGFPDVISLFNHTRAEVREEAIHLVGSAAQSNPPVQIKIIELGVLPKLLKILADEGESPAVRKKSLFAISSIVRHFPLAQQKLGEFGGIQVLMQLFQQDKTSSFRMKAIRLVDDLIMERLTTNQGTDKMKQYDKLPLFTKLQELDWCHLVVESLNSDSHEHREISMNAMNNLRPACPRGFDSKPAMKKLKQLKEEYKSLWNAEVADGDDDGYFKLLYNLVGEVTDNWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>F7B783</accession>
    <name>F7B783_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01001407">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F7B783"/>
    <dbReference type="Ensembl" id="ENSCINT00000008369.3">
        <property type="protein sequence ID" value="ENSCINP00000008369.3"/>
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        <property type="component" value="Chromosome 2"/>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="14"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
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            <end position="74"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    <sequence length="130" mass="14916" checksum="01A75EC04C2B4E50" modified="2011-07-27" version="1">MVKQHIALWDSRATNSRQVTNTSTSRGLRKLVHTPSSLLASEHQQPYNSQQFPHIAPNNERNTRSTNNYQYQQNSRHERKNPIDYSLEEQLHSDLHISGGNSLMYEATTPRTQTHTLGPIKSAKRRKGPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
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    <accession>A0A1W2WE15</accession>
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            <fullName evidence="8">Transmembrane protein 17B-like</fullName>
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        <name evidence="8" type="primary">LOC100183084</name>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
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            <taxon>Phlebobranchia</taxon>
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    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
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    <dbReference type="STRING" id="7719.ENSCINP00000010101"/>
    <dbReference type="Ensembl" id="ENSCINT00000010101.3">
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    <dbReference type="KEGG" id="cin:100183084"/>
    <dbReference type="GeneTree" id="ENSGT00940000153899"/>
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    <dbReference type="OrthoDB" id="311720at2759"/>
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        <property type="term" value="C:ciliary transition zone"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019184">
        <property type="entry name" value="Uncharacterised_TM-17"/>
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        <property type="entry name" value="GEO07735P1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR13531:SF6">
        <property type="entry name" value="TMEM (HUMAN TRANSMEMBRANE PROTEIN) HOMOLOG"/>
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    </dbReference>
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    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
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            <end position="76"/>
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        <location>
            <begin position="152"/>
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        <source>
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    </evidence>
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        <source>
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        <source>
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        <source>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
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        </source>
    </evidence>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
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    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM227 family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR33560:SF2">
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    <feature type="compositionally biased region" description="Polar residues" evidence="2">
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    <evidence type="ECO:0000256" key="1">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000006888.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
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            <fullName evidence="2 7">Anaphase-promoting complex subunit 10</fullName>
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    <organism evidence="9 10">
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        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">wild type</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin-protein ligase complex that controls progression through mitosis and the G1 phase of the cell cycle.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the APC10 family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001519">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002131938.1">
        <property type="nucleotide sequence ID" value="XM_002131902.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F7BB49"/>
    <dbReference type="FunCoup" id="F7BB49">
        <property type="interactions" value="203"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000018593"/>
    <dbReference type="Ensembl" id="ENSCINT00000018593.3">
        <property type="protein sequence ID" value="ENSCINP00000018593.3"/>
        <property type="gene ID" value="ENSCING00000009153.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100176838"/>
    <dbReference type="KEGG" id="cin:100176838"/>
    <dbReference type="GeneTree" id="ENSGT00390000013722"/>
    <dbReference type="HOGENOM" id="CLU_039415_3_0_1"/>
    <dbReference type="InParanoid" id="F7BB49"/>
    <dbReference type="OMA" id="DDCMDTY"/>
    <dbReference type="OrthoDB" id="24948at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070979">
        <property type="term" value="P:protein K11-linked ubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="APC10"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016901">
        <property type="entry name" value="APC10/Doc1"/>
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    <dbReference type="InterPro" id="IPR004939">
        <property type="entry name" value="APC_su10/DOC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12936">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12936:SF0">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03256">
        <property type="entry name" value="ANAPC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028841">
        <property type="entry name" value="APC10_sub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01337">
        <property type="entry name" value="APC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51284">
        <property type="entry name" value="DOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3 7" id="KW-0132">Cell division</keyword>
    <keyword evidence="4 7" id="KW-0498">Mitosis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="DOC" evidence="8">
        <location>
            <begin position="10"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR028841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000018593.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="202" mass="22737" checksum="7FBF7E2D95924056" modified="2011-07-27" version="1">MADDVSSADATEVANYVILSDIRELLEKESLREIGHQAVWSVSSCKQGYGVNSLRDNSLETYWQSDGTQPHMVNVQFKQKTTIHSVGVYTDYKADESYTPSRLSVSVGNDFNDLSEVEQVEVNEPSGWVWIKLPNQVGDKPIRTFMAQISVLQNHQNGRDTHMRQIKVFASTKDTTSIDYMISGCRLPMFSSVEAAMFSTIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>F7BD94</accession>
    <name>F7BD94_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Enhancer of mRNA-decapping protein 4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">wild type</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">P-body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat EDC4 family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001655">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="FunCoup" id="F7BD94">
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    <dbReference type="STRING" id="7719.ENSCINP00000011365"/>
    <dbReference type="Ensembl" id="ENSCINT00000011365.3">
        <property type="protein sequence ID" value="ENSCINP00000011365.3"/>
        <property type="gene ID" value="ENSCING00000005502.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00510000047791"/>
    <dbReference type="HOGENOM" id="CLU_005166_0_0_1"/>
    <dbReference type="InParanoid" id="F7BD94"/>
    <dbReference type="OMA" id="TREHMGT"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031087">
        <property type="term" value="P:deadenylation-independent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.100:FF:000001">
        <property type="entry name" value="Enhancer of mRNA-decapping protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.100">
        <property type="entry name" value="conserved c-terminal region of ge- 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045152">
        <property type="entry name" value="EDC4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049404">
        <property type="entry name" value="EDC4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044938">
        <property type="entry name" value="EDC4_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032401">
        <property type="entry name" value="EDC4_WD40"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15598">
        <property type="entry name" value="ENHANCER OF MRNA-DECAPPING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15598:SF5">
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    <dbReference type="SUPFAM" id="SSF50978">
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    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
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            <end position="364"/>
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            <end position="255"/>
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            <end position="1339"/>
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    </feature>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>F7BEI2</accession>
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        <recommendedName>
            <fullName evidence="10">G-protein coupled receptors family 2 profile 1 domain-containing protein</fullName>
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    <organism evidence="11 12">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Tunicata</taxon>
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            <taxon>Phlebobranchia</taxon>
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            <taxon>Ciona</taxon>
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    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="11" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">wild type</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="11" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
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    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 2 family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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            <end position="23"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>F7BH60</accession>
    <name>F7BH60_CIOIN</name>
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            <fullName evidence="13">THAP-type domain-containing protein</fullName>
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        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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            <taxon>Phlebobranchia</taxon>
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            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the THAP1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="F7BH60"/>
    <dbReference type="Ensembl" id="ENSCINT00000026893.2">
        <property type="protein sequence ID" value="ENSCINP00000026647.2"/>
        <property type="gene ID" value="ENSCING00000014832.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01060000251798"/>
    <dbReference type="HOGENOM" id="CLU_1302157_0_0_1"/>
    <dbReference type="InParanoid" id="F7BH60"/>
    <dbReference type="OMA" id="TXSSSIS"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.210.20">
        <property type="entry name" value="THAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026516">
        <property type="entry name" value="THAP1/10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006612">
        <property type="entry name" value="THAP_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038441">
        <property type="entry name" value="THAP_Znf_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46600">
        <property type="entry name" value="THAP DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46600:SF1">
        <property type="entry name" value="THAP DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05485">
        <property type="entry name" value="THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00692">
        <property type="entry name" value="DM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00980">
        <property type="entry name" value="THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50950">
        <property type="entry name" value="ZF_THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="THAP-type" evidence="13">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000026647.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="212" mass="23959" checksum="8427AF4D844E56A5" modified="2012-04-18" version="2">MPNRCVVGGCLNTYQNKVKIHKFPKSPILKQCWVKFVQCTRADFEQPSKNSVICGKHFKPDCYWKKFGSSTELLLESAIPTEKSKKAAPATQKRKWTVAFTKQSRKKTKLLDEVLGCSPTSNAAIDESPNENVSEETLSVPEKDDKCIQTETAQHNQKTQTSLRRGKHRKCKKTFIDTPILSCLPPLTSNYLQPDKDVSNAAHALLQIKFFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>F7BHG2</accession>
    <accession>A0A1W2WFZ3</accession>
    <name>F7BHG2_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Inhibitor of growth protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC100183795</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Component of an histone acetyltransferase complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Component of an histone acetyltransferase complex. Interacts with H3K4me3 and to a lesser extent with H3K4me2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="14">The PHD-type zinc finger mediates the binding to H3K4me3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the ING family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002126978.1">
        <property type="nucleotide sequence ID" value="XM_002126942.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F7BHG2"/>
    <dbReference type="FunCoup" id="F7BHG2">
        <property type="interactions" value="254"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000007229"/>
    <dbReference type="Ensembl" id="ENSCINT00000007229.3">
        <property type="protein sequence ID" value="ENSCINP00000007229.3"/>
        <property type="gene ID" value="ENSCING00000003519.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100183795"/>
    <dbReference type="KEGG" id="cin:100183795"/>
    <dbReference type="GeneTree" id="ENSGT00940000156619"/>
    <dbReference type="HOGENOM" id="CLU_031900_0_0_1"/>
    <dbReference type="InParanoid" id="F7BHG2"/>
    <dbReference type="OMA" id="YEWFHWK"/>
    <dbReference type="OrthoDB" id="5411773at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16858">
        <property type="entry name" value="ING_ING3_Yng2p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15585">
        <property type="entry name" value="PHD_ING3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000103">
        <property type="entry name" value="Inhibitor of growth protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042020">
        <property type="entry name" value="ING3_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028651">
        <property type="entry name" value="ING_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024610">
        <property type="entry name" value="ING_N_histone-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10333">
        <property type="entry name" value="INHIBITOR OF GROWTH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10333:SF103">
        <property type="entry name" value="INHIBITOR OF GROWTH PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12998">
        <property type="entry name" value="ING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01408">
        <property type="entry name" value="ING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="3" id="KW-0341">Growth regulation</keyword>
    <keyword evidence="4 12 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6 12 14" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 13 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="16">
        <location>
            <begin position="344"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="125"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="152"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="347"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="349"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="360"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="365"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="371"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="374"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="387"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="390"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="11">
        <location>
            <position position="346"/>
        </location>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="11">
        <location>
            <position position="357"/>
        </location>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="11">
        <location>
            <position position="361"/>
        </location>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="11">
        <location>
            <position position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR628651-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR628651-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU361213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000007229.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="401" mass="45984" checksum="62F41975DC8B9240" modified="2011-07-27" version="1">MLYLEDYIELIEHLPSDLRERFTDMREMDLQVQNSVDSLDDKVKKLFQKCFMKKTKITGADQVYEEIRNEYYKALEDADEKVQLANQIYDLVDRHLRKLDQELSKFKMELEADNAGITEVLEQRSLELDNPQSQQRTSLQKERRKHAVGGTPHTSTSNTVTINNGDGPPEKKIKSEQILKSELLAHLQDNSNLEYTTKVLNEVAEIGGREVAGFPIHYHEVENEMKENQSSAQPTRRTSLVKHEIKIESRTSEYDRISVDSLDANEQVSDSSICSATGGAVHRPFRAKKQTSKAAAWVQQRQQAEARLEQRKKETATVTDSNDPGVEVIGESSNVEWNFDPNEPRYCLCNQVSYGEMVGCDNDKCPIEWFHYGCVGLEQAPTGKWFCPQCTQTMKRRNKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>F7BPJ6</accession>
    <name>F7BPJ6_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 13">Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase</fullName>
            <ecNumber evidence="3 13">2.4.1.135</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">wild type</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10 13">
            <text>3-O-(beta-D-galactosyl-(1-&gt;3)-beta-D-galactosyl-(1-&gt;4)-beta-D-xylosyl)-L-seryl-[protein] + UDP-alpha-D-glucuronate = 3-O-(beta-D-GlcA-(1-&gt;3)-beta-D-Gal-(1-&gt;3)-beta-D-Gal-(1-&gt;4)-beta-D-Xyl)-L-seryl-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24168"/>
            <dbReference type="Rhea" id="RHEA-COMP:12571"/>
            <dbReference type="Rhea" id="RHEA-COMP:12573"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58052"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:132090"/>
            <dbReference type="ChEBI" id="CHEBI:132093"/>
            <dbReference type="EC" id="2.4.1.135"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11 13">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="13">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Golgi apparatus membrane</location>
            <topology evidence="13">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the glycosyltransferase 43 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.135" evidence="3 13"/>
    <dbReference type="EMBL" id="EAAA01002161">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F7BPJ6"/>
    <dbReference type="FunCoup" id="F7BPJ6">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSCINT00000023157.2">
        <property type="protein sequence ID" value="ENSCINP00000022911.2"/>
        <property type="gene ID" value="ENSCING00000012206.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166666"/>
    <dbReference type="HOGENOM" id="CLU_045177_4_0_1"/>
    <dbReference type="InParanoid" id="F7BPJ6"/>
    <dbReference type="OMA" id="ITHENPG"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015018">
        <property type="term" value="F:galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050650">
        <property type="term" value="P:chondroitin sulfate proteoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005027">
        <property type="entry name" value="Glyco_trans_43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10896:SF65">
        <property type="entry name" value="GALACTOSYLGALACTOSYLXYLOSYLPROTEIN 3-BETA-GLUCURONOSYLTRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10896">
        <property type="entry name" value="GALACTOSYLGALACTOSYLXYLOSYLPROTEIN 3-BETA-GLUCURONOSYLTRANSFERASE BETA-1,3-GLUCURONYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03360">
        <property type="entry name" value="Glyco_transf_43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="11 13" id="KW-0464">Manganese</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="site" description="Interaction with galactose moiety of substrate glycoprotein" evidence="12">
        <location>
            <position position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR605027-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR605027-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000022911.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="207" mass="23471" checksum="A9A78694E480332D" modified="2012-04-18" version="2">MTKRTKLGCFCAGSVFFFAVMIALCGFLALKVKDFPAIGTRKRTVTVVTCTYTRLTQKADLTRLVQTLMHVSSLHWIVVEDSDKKTPLVKKLLENSSLKYTHLYTKNTAAIFKHIQTLNIALAWIRANVKPNEGVVYFMDDDNTYDIKLFEEIRTTKVLSVWPVGLVGGLLIEGPVECKNDRVLTWRVSWRPDRTIPIDMAGFAINI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>H2XKK7</accession>
    <name>H2XKK7_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Zinc transporter protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">wild type</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>Zn(2+)(in) + 2 H(+)(out) = Zn(2+)(out) + 2 H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:72627"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. SLC30A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002383">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XKK7"/>
    <dbReference type="FunCoup" id="H2XKK7">
        <property type="interactions" value="13"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000030189"/>
    <dbReference type="Ensembl" id="ENSCINT00000032956.1">
        <property type="protein sequence ID" value="ENSCINP00000030189.1"/>
        <property type="gene ID" value="ENSCING00000011998.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000172026"/>
    <dbReference type="InParanoid" id="H2XKK7"/>
    <dbReference type="OMA" id="CFTIFVD"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010312">
        <property type="term" value="P:detoxification of zinc ion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006882">
        <property type="term" value="P:intracellular zinc ion homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071577">
        <property type="term" value="P:zinc ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="entry name" value="Cation efflux protein transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002524">
        <property type="entry name" value="Cation_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027470">
        <property type="entry name" value="Cation_efflux_CTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036837">
        <property type="entry name" value="Cation_efflux_CTD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058533">
        <property type="entry name" value="Cation_efflux_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01297">
        <property type="entry name" value="CDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45820">
        <property type="entry name" value="FI23527P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45820:SF4">
        <property type="entry name" value="ZINC TRANSPORTER 63C, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16916">
        <property type="entry name" value="ZT_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160240">
        <property type="entry name" value="Cation efflux protein cytoplasmic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="Cation efflux protein transmembrane domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0050">Antiport</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="46"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="84"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="116"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="229"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="259"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein transmembrane" evidence="12">
        <location>
            <begin position="16"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein cytoplasmic" evidence="13">
        <location>
            <begin position="295"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="144"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="411"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="149"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="424"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF16916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000030189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="436" mass="48058" checksum="572F6327756277B1" modified="2012-04-18" version="1">MTFTCKINRGPKFSLICMLIMTSAFFFTEIVVGYSTNSTALVADSFHMLSDVVSLVVGLVAVIYSNKTSKTNTYGWARAEVLGALCNAVFLLSLCFSIVIEAIQRLVEVEPITEPLLVLGVGSAGLAINLIGLLLFHEHAHGHNHSHGHSHDSHDVKNEHSHDHDDDPEGGEIEIYYNSKISTELYNRSPAAVKHGIPSYPTILYINGNTEPKVRVTAAKRGHLNMKGVFLHVLGDALGSVVVIISALIVNYVQDSWRFYVDPVMSLFIALIIVCSTLPLLKQSAYILLQRPPAHINADELESRLTKIKGVVSVHDLHIWQLSSNQAIASAHLTMHSEDDFGETAHRLRHVFHEAGVHSLTLQPEVYPCKKGMSVCGISDCQYLKEEVHQLLPSMLEEIKNNENADAECTENRCCTPPSEHSDNSPIHTISTTSSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>H2XLK9</accession>
    <accession>A0A1W2WJ05</accession>
    <name>H2XLK9_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Trafficking protein particle complex subunit 6B-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100183093</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">cis-Golgi network</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAPP small subunits family. BET3 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001228">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002131721.1">
        <property type="nucleotide sequence ID" value="XM_002131685.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XLK9"/>
    <dbReference type="FunCoup" id="H2XLK9">
        <property type="interactions" value="119"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000030541"/>
    <dbReference type="Ensembl" id="ENSCINT00000036602.1">
        <property type="protein sequence ID" value="ENSCINP00000030541.1"/>
        <property type="gene ID" value="ENSCING00000021998.1"/>
    </dbReference>
    <dbReference type="GeneID" id="100183093"/>
    <dbReference type="KEGG" id="cin:100183093"/>
    <dbReference type="GeneTree" id="ENSGT00390000012948"/>
    <dbReference type="HOGENOM" id="CLU_076409_3_1_1"/>
    <dbReference type="InParanoid" id="H2XLK9"/>
    <dbReference type="OMA" id="CKEFWTA"/>
    <dbReference type="OrthoDB" id="941624at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005801">
        <property type="term" value="C:cis-Golgi network"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030008">
        <property type="term" value="C:TRAPP complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14944">
        <property type="entry name" value="TRAPPC6A_Trs33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1380.20:FF:000008">
        <property type="entry name" value="trafficking protein particle complex subunit 6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1380.20">
        <property type="entry name" value="Trafficking protein particle complex subunit 3"/>
        <property type="match status" value="1"/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
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                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
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                <person name="Detter C."/>
                <person name="Doggett N."/>
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                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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                <person name="Matsumoto J."/>
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        <scope>IDENTIFICATION</scope>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
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                <person name="Francino P."/>
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                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
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                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">wild type</strain>
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    <reference evidence="8" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
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    <comment type="similarity">
        <text evidence="5">Belongs to the UTP23/FCF1 family. FCF1 subfamily.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome 11"/>
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        <location>
            <begin position="70"/>
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        <source>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
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    <reference evidence="21" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
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                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
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                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
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                <person name="Hayashi H."/>
                <person name="Hino K."/>
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                <person name="Kobayashi M."/>
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                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
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                <person name="Miura S."/>
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                <person name="Nomoto H."/>
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                <person name="Oishi K."/>
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                <person name="Shoguchi E."/>
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                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">wild type</strain>
        </source>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>UDP-alpha-D-glucuronate + H(+) = UDP-alpha-D-xylose + CO2</text>
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            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57632"/>
            <dbReference type="ChEBI" id="CHEBI:58052"/>
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        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:23917"/>
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    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
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    </comment>
    <comment type="pathway">
        <text evidence="3">Nucleotide-sugar biosynthesis; UDP-alpha-D-xylose biosynthesis; UDP-alpha-D-xylose from UDP-alpha-D-glucuronate: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
            <location evidence="2">Golgi stack membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the NAD(P)-dependent epimerase/dehydratase family. UDP-glucuronic acid decarboxylase subfamily.</text>
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        <property type="gene ID" value="ENSCING00000024012.1"/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
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                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
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                <person name="Wiley G.B."/>
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                <person name="Roe B.A."/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
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                <person name="Francino P."/>
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                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
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                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="H2XT46"/>
    <dbReference type="Ensembl" id="ENSCINT00000033359.1">
        <property type="protein sequence ID" value="ENSCINP00000032830.1"/>
        <property type="gene ID" value="ENSCING00000023789.1"/>
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        <property type="entry name" value="DD_LRRD1"/>
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    <dbReference type="Pfam" id="PF24978">
        <property type="entry name" value="Death_Lrrd1"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LRRD1 death" evidence="1">
        <location>
            <begin position="7"/>
            <end position="92"/>
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    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF24978"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
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    <sequence length="101" mass="11939" checksum="5904F81CB865446E" modified="2012-04-18" version="1">MQEYVLIKMLRYAATKCPEDDFERICQKFKINGDDVTTIMSQEGKSFRDRLFKLFTIWRERQPVAPDVVQQKFIHAIDLVDLPRLVSQLRAITTYTKATRL</sequence>
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<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>H2XTQ1</accession>
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        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
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    </protein>
    <organism evidence="9 10">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
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    <reference evidence="10" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
            <orientation evidence="1">Nucleoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NEMP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="H2XTQ1"/>
    <dbReference type="STRING" id="7719.ENSCINP00000033035"/>
    <dbReference type="Ensembl" id="ENSCINT00000033146.1">
        <property type="protein sequence ID" value="ENSCINP00000033035.1"/>
        <property type="gene ID" value="ENSCING00000021146.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000002174"/>
    <dbReference type="HOGENOM" id="CLU_1772251_0_0_1"/>
    <dbReference type="InParanoid" id="H2XTQ1"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
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    <dbReference type="GO" id="GO:0005637">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="entry name" value="NEMP_fam"/>
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    <dbReference type="PANTHER" id="PTHR13598">
        <property type="entry name" value="AT07567P-RELATED"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="85"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="112"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00005748"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000033035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    </evidence>
    <sequence length="147" mass="16357" checksum="96BA7D6D9E1CFC69" modified="2012-04-18" version="1">MMFGCLHIKVSSKTGDFDFGLSSSRESVDSSWFTWIMSIGQIFKTKVSSSSVTKENCVSPYNAIFLTIKTHEQNQTFDVETHLKYIDMTCCVLFIIGITMFYMASAMASSVVFYYTTGVSIGVIGSLIVLVFIISRYLPKKPAAYGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>H2XTQ6</accession>
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        <recommendedName>
            <fullName evidence="8">SPOC domain-containing protein</fullName>
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    <organism evidence="9 10">
        <name type="scientific">Ciona intestinalis</name>
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    <reference evidence="10" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">wild type</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="SMR" id="H2XTQ6"/>
    <dbReference type="Ensembl" id="ENSCINT00000036437.1">
        <property type="protein sequence ID" value="ENSCINP00000033040.1"/>
        <property type="gene ID" value="ENSCING00000019435.1"/>
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    <dbReference type="GeneTree" id="ENSGT00940000157087"/>
    <dbReference type="HOGENOM" id="CLU_057047_1_0_1"/>
    <dbReference type="InParanoid" id="H2XTQ6"/>
    <dbReference type="OMA" id="MFHARAG"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd21543">
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        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="2.40.290.10:FF:000002">
        <property type="entry name" value="Spen family transcriptional repressor"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="2.40.290.10">
        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR016194">
        <property type="entry name" value="SPOC-like_C_dom_sf"/>
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    <dbReference type="InterPro" id="IPR012921">
        <property type="entry name" value="SPOC_C"/>
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    <dbReference type="InterPro" id="IPR010912">
        <property type="entry name" value="SPOC_met"/>
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                <person name="Wiley G.B."/>
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                <person name="Matsumoto J."/>
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        <property type="gene ID" value="ENSCING00000021348.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000064880"/>
    <dbReference type="HOGENOM" id="CLU_1158632_0_0_1"/>
    <dbReference type="InParanoid" id="H2XWI6"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35169">
        <property type="entry name" value="FE2OG DIOXYGENASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35169:SF3">
        <property type="entry name" value="PROLYL 4-HYDROXYLASE ALPHA SUBUNIT FE(2+) 2OG DIOXYGENASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003577538" evidence="1">
        <location>
            <begin position="22"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000034020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="240" mass="27342" checksum="46517164EAD24060" modified="2012-04-18" version="1">MLVKTVCCLLVCGLNILNVESRNDVNVHYYSNKLTEPEFITSHLQAEYTSKTGRKVYVFDNVIPYSLLSATRQYFSMDDHWRFVQFDEHIKDPNAKYDNGNGVPWKSWQDPTLMSATRIGHILKAMVDTVSANVTSMKPPLTIDQGFKMTEIYSGIVRRGDQTLVHSDTPPPNCTSVVNGYSTVTYTNTLWRKNSYGEMLFYEDNLEELDIFAAVSARFGRVIVWDSAIQYITRPPGIAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>H2XWR8</accession>
    <name>H2XWR8_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">m7GpppN-mRNA hydrolase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">mRNA-decapping enzyme 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">wild type</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Decapping metalloenzyme that catalyzes the cleavage of the cap structure on mRNAs. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Plays a role in replication-dependent histone mRNA degradation. Has higher activity towards mRNAs that lack a poly(A) tail. Has no activity towards a cap structure lacking an RNA moiety. The presence of a N(6)-methyladenosine methylation at the second transcribed position of mRNAs (N(6),2'-O-dimethyladenosine cap; m6A(m)) provides resistance to DCP2-mediated decapping. Blocks autophagy in nutrient-rich conditions by repressing the expression of ATG-related genes through degradation of their transcripts.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in mRNA + H2O = N(7)-methyl-GDP + a 5'-end phospho-ribonucleoside in mRNA + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:67484"/>
            <dbReference type="Rhea" id="RHEA-COMP:15692"/>
            <dbReference type="Rhea" id="RHEA-COMP:17167"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:63714"/>
            <dbReference type="ChEBI" id="CHEBI:138282"/>
            <dbReference type="ChEBI" id="CHEBI:156461"/>
            <dbReference type="EC" id="3.6.1.62"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:67485"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">P-body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the Nudix hydrolase family. DCP2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002384">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XWR8"/>
    <dbReference type="SMR" id="H2XWR8"/>
    <dbReference type="FunCoup" id="H2XWR8">
        <property type="interactions" value="82"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000034102"/>
    <dbReference type="Ensembl" id="ENSCINT00000030771.1">
        <property type="protein sequence ID" value="ENSCINP00000034102.1"/>
        <property type="gene ID" value="ENSCING00000023586.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000018878"/>
    <dbReference type="HOGENOM" id="CLU_008108_2_0_1"/>
    <dbReference type="InParanoid" id="H2XWR8"/>
    <dbReference type="OMA" id="PIRLCFQ"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140933">
        <property type="term" value="F:5'-(N(7)-methylguanosine 5'-triphospho)-[mRNA] hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000290">
        <property type="term" value="P:deadenylation-dependent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03672">
        <property type="entry name" value="NUDIX_Dcp2p_Nudt20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1050:FF:000001">
        <property type="entry name" value="M7GpppN-mRNA hydrolase isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.79.10:FF:000003">
        <property type="entry name" value="M7GpppN-mRNA hydrolase isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1050">
        <property type="entry name" value="Dcp2, box A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007722">
        <property type="entry name" value="DCP2_BoxA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036189">
        <property type="entry name" value="DCP2_BoxA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044099">
        <property type="entry name" value="Dcp2_NUDIX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23114">
        <property type="entry name" value="M7GPPPN-MRNA HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23114:SF17">
        <property type="entry name" value="M7GPPPN-MRNA HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05026">
        <property type="entry name" value="DCP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01125">
        <property type="entry name" value="DCP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140586">
        <property type="entry name" value="Dcp2 domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0464">Manganese</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="17">
        <location>
            <begin position="94"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00060003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00068566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00078183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000034102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="235" mass="27573" checksum="BDE56DBC69CC98D0" modified="2012-04-18" version="1">MSKLKISPNVLDDLCSRFLLNIPSQEKEDMIRLCFQIELAHWFYLDFFRAENNRLPDCRMKEFAKLVFETYPFLLNPSDVNVDKVLESWKEYKRSVPTYGAILMDSTLEYVLLVQGFWIKASWGFPKGKVNKDEQPEICAAREVLEETGYDITKSIKPNQYAEHLLNEQLSRLYFVRDVPLDTKFGPKTRGEIKNVQWFSVSDLPAHKKDQTPKQNLNIAANCFFMVIPFMKQLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>H2XX39</accession>
    <name>H2XX39_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
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        <scope>IDENTIFICATION</scope>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
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                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1">wild type</strain>
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    <reference evidence="1" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="H2XXU2"/>
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    <sequence length="145" mass="16744" checksum="5391BD27E809FBBB" modified="2012-04-18" version="1">MRIYSSGSCNIPSLQLLHPSSHQMKHKRRRFQNLRLDLRKDSIIVNLLLSLTPSPHYPPVKIPAHKVSYPLSMVHKKAEPPQSPSLPLCMTGMFHLNLHYGTKHTTPSMIRTSQHFSLTTVPLLLFLKKKQWKLYGKFLQSLLLQ</sequence>
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<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>H2XY38</accession>
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            <fullName evidence="6">Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase</fullName>
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            <fullName evidence="13">Peptide:N-glycanase</fullName>
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        <name evidence="17" type="primary">LOC100181653</name>
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    <reference evidence="18" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
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                <person name="Hino K."/>
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                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
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                <person name="Mount S."/>
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                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
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                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="17">wild type</strain>
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    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="17" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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    <comment type="function">
        <text evidence="11">Specifically deglycosylates the denatured form of N-linked glycoproteins in the cytoplasm and assists their proteasome-mediated degradation. Cleaves the beta-aspartyl-glucosamine (GlcNAc) of the glycan and the amide side chain of Asn, converting Asn to Asp. Prefers proteins containing high-mannose over those bearing complex type oligosaccharides. Can recognize misfolded proteins in the endoplasmic reticulum that are exported to the cytosol to be destroyed and deglycosylate them, while it has no activity toward native proteins. Deglycosylation is a prerequisite for subsequent proteasome-mediated degradation of some, but not all, misfolded glycoproteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of an N(4)-(acetyl-beta-D-glucosaminyl)asparagine residue in which the glucosamine residue may be further glycosylated, to yield a (substituted) N-acetyl-beta-D-glucosaminylamine and a peptide containing an aspartate residue.</text>
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            <name>Zn(2+)</name>
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        </cofactor>
    </comment>
    <comment type="subcellular location">
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            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the transglutaminase-like superfamily. PNGase family.</text>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Goodstein D.M."/>
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                <person name="Huang W."/>
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                <person name="Lemaire P."/>
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                <person name="Necula S."/>
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                <person name="Saiga H."/>
                <person name="Satake M."/>
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                <person name="Wang H.G."/>
                <person name="Awazu S."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
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                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
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                <person name="Lindquist E."/>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000034933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="90" mass="10577" checksum="6F4691B57A40607C" modified="2012-04-18" version="1">MADNVLFFGAGYSDPLTVVYIFFQFSFSFLEILNILFQLPFLFCEFQFFYIFLTLLELRFVSIAHFVTSKIRSKFATKRKAATTSMLRVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>H2XZ73</accession>
    <name>H2XZ73_CIOIN</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
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    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01000595">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XZ73"/>
    <dbReference type="STRING" id="7719.ENSCINP00000034957"/>
    <dbReference type="Ensembl" id="ENSCINT00000032710.1">
        <property type="protein sequence ID" value="ENSCINP00000034957.1"/>
        <property type="gene ID" value="ENSCING00000019103.1"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1017516_0_0_1"/>
    <dbReference type="InParanoid" id="H2XZ73"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="58"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000034957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="274" mass="29288" checksum="732E32280C46436C" modified="2012-04-18" version="1">YFTAKSEEDLNAGKPNGVAAIVVHATDKGNKELVESSGGVDRVVLGQDLNAQSPLLVETEKRSTKSSENENRAKNLHNSHSTDAAILLVNEESNQPIGTANVTDALLTSTPALYVITNDDDVIETAVKEAAATPLKKRNPPVRRFSLGGIPKIFISPATPRASPISGKRKMSLLSCTRVSSIDYALTSEDIEELDLTTRTLGEVCDVRGAIILSYHDVTMKVRVIPKVKEAIGYCYGGVTPKYDKSSVETGDKIILNGVSGVFHPGINAIMGPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>H2XZC2</accession>
    <name>H2XZC2_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Zinc finger PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">wild type</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01000987">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XZC2"/>
    <dbReference type="STRING" id="7719.ENSCINP00000035006"/>
    <dbReference type="Ensembl" id="ENSCINT00000035301.1">
        <property type="protein sequence ID" value="ENSCINP00000035006.1"/>
        <property type="gene ID" value="ENSCING00000020804.1"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1135663_0_0_1"/>
    <dbReference type="InParanoid" id="H2XZC2"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055198">
        <property type="entry name" value="NSD_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46235">
        <property type="entry name" value="PHD FINGER-CONTAINING PROTEIN DDB_G0268158"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46235:SF3">
        <property type="entry name" value="PHD FINGER-CONTAINING PROTEIN DDB_G0268158"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22908">
        <property type="entry name" value="PHD_NSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Zinc finger PHD-type" evidence="5">
        <location>
            <begin position="144"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="Zinc finger PHD-type" evidence="5">
        <location>
            <begin position="195"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="89"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="90"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="107"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000035006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="245" mass="26638" checksum="2553048439FEA170" modified="2012-04-18" version="1">MFKCSSCPASYRSSHSLGQHKRWCAGIAAANKGSSVLNKNSKEVSSPMKNNGDISFSCDGCKESFFDKRSLKIHKKFCSASTQTDSAVQSPASKSSALLSRKKSIQQDDTSSNGSKADSEDAPGIKRLKSRGPQLGSNTKRENICSKCESASDEEELVHCIGGCCASFHPRCLEEGAITSIVSFTCKLCKEDNHPCFICKKSSSNDDSLGEIKRCSVPKCGRSYHTTCLTERKIALLQHDDDDQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>H2Y1Q9</accession>
    <name>H2Y1Q9_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Chapman J."/>
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                <person name="De Tomaso A."/>
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                <person name="Di Gregorio A."/>
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                <person name="Goodstein D.M."/>
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                <person name="Hastings K.E."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
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                <person name="Wiley G.B."/>
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                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01001686">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="Ensembl" id="ENSCINT00000034358.1">
        <property type="protein sequence ID" value="ENSCINP00000036508.1"/>
        <property type="gene ID" value="ENSCING00000022020.1"/>
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        <property type="component" value="Chromosome 3"/>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="48"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000036508.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="228" mass="25966" checksum="F15D0861F0709B35" modified="2012-04-18" version="1">MVHIHNRKLVAKLAAISDAAEDVVDQILFTREDEAMIGWRCTKPYTTRRTNFGKTKSKKEDSQLNKKSQLPPLPLQWKNIANFATEAVKPVIPKSERKNGEKLQRKKSTALNKKKLEKLISMTTHYSVQDSSLNKEATNGETIQPTVHEKPNKRSLSPVEAERRFLPTFMNQIPNLDRELLEDKRILKRLRTISSSSKRYGATQIALHYGLSGPELRGNLNTPKLPTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2003-03-01" modified="2026-01-28" version="123" xmlns="https://uniprot.org/uniprot">
    <accession>Q8HIN6</accession>
    <accession>A0A1W2W298</accession>
    <name>Q8HIN6_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 17">Cytochrome c oxidase subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">COII</name>
    </gene>
    <organism evidence="21">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <geneLocation type="mitochondrion" evidence="21 22"/>
    <reference evidence="21 23" key="1">
        <citation type="journal article" date="2004" name="J. Mol. Evol." volume="58" first="376" last="389">
            <title>Complete mtDNA of Ciona intestinalis reveals extensive gene rearrangement and the presence of an atp8 and an extra trnM gene in ascidians.</title>
            <authorList>
                <person name="Gissi C."/>
                <person name="Iannelli F."/>
                <person name="Pesole G."/>
            </authorList>
            <dbReference type="PubMed" id="15114417"/>
            <dbReference type="DOI" id="10.1007/s00239-003-2559-6"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="journal article" date="2015" name="J. Zool. Syst. Evol. Res." volume="53" first="185" last="257">
            <title>Morphological evidence that the molecularly determined Ciona robusta type A and type B are different species: Ciona robusta and Ciona robusta.</title>
            <authorList>
                <person name="Brunetti R."/>
                <person name="Gissi C."/>
                <person name="Pennati R."/>
                <person name="Caicci F."/>
                <person name="Gasparini F."/>
                <person name="Manni L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="journal article" date="2015" name="PLoS ONE" volume="10" first="e0122879" last="e0122879">
            <title>Morphological Differences between Larvae of the Ciona intestinalis Species Complex: Hints for a Valid Taxonomic Definition of Distinct Species.</title>
            <authorList>
                <person name="Pennati R."/>
                <person name="Ficetola G.F."/>
                <person name="Brunetti R."/>
                <person name="Caicci F."/>
                <person name="Gasparini F."/>
                <person name="Griggio F."/>
                <person name="Sato A."/>
                <person name="Stach T."/>
                <person name="Kaul-Strehlow S."/>
                <person name="Gissi C."/>
                <person name="Manni L."/>
            </authorList>
            <dbReference type="PubMed" id="25955391"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0122879"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="22" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>4 Fe(II)-[cytochrome c] + O2 + 8 H(+)(in) = 4 Fe(III)-[cytochrome c] + 2 H2O + 4 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:11436"/>
            <dbReference type="Rhea" id="RHEA-COMP:10350"/>
            <dbReference type="Rhea" id="RHEA-COMP:14399"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="7.1.1.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:11437"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
        <text evidence="17">Binds a copper A center.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 17">Mitochondrion inner membrane</location>
            <topology evidence="1 17">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 17">Belongs to the cytochrome c oxidase subunit 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AJ517314">
        <property type="protein sequence ID" value="CAD56915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8HIN6"/>
    <dbReference type="STRING" id="7719.ENSCINP00000036591"/>
    <dbReference type="Ensembl" id="ENSCINT00000037366.1">
        <property type="protein sequence ID" value="ENSCINP00000036591.1"/>
        <property type="gene ID" value="ENSCING00000025203.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000017410"/>
    <dbReference type="HOGENOM" id="CLU_036876_2_3_1"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Mitochondrion MT"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045277">
        <property type="term" value="C:respiratory chain complex IV"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004129">
        <property type="term" value="F:cytochrome-c oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd13912">
        <property type="entry name" value="CcO_II_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000001">
        <property type="entry name" value="Cytochrome c oxidase subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045187">
        <property type="entry name" value="CcO_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002429">
        <property type="entry name" value="CcO_II-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034210">
        <property type="entry name" value="CcO_II_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001505">
        <property type="entry name" value="Copper_CuA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011759">
        <property type="entry name" value="Cyt_c_oxidase_su2_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036257">
        <property type="entry name" value="Cyt_c_oxidase_su2_TM_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22888:SF9">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22888">
        <property type="entry name" value="CYTOCHROME C OXIDASE, SUBUNIT II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00116">
        <property type="entry name" value="COX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02790">
        <property type="entry name" value="COX2_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01166">
        <property type="entry name" value="CYCOXIDASEII"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81464">
        <property type="entry name" value="Cytochrome c oxidase subunit II-like, transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00078">
        <property type="entry name" value="COX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50857">
        <property type="entry name" value="COX2_CUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50999">
        <property type="entry name" value="COX2_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 17" id="KW-0186">Copper</keyword>
    <keyword evidence="11 17" id="KW-0249">Electron transport</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15 17 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14 17 21" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8 17" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 17" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="10" id="KW-1278">Translocase</keyword>
    <keyword evidence="6 17 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 17" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="63"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome oxidase subunit II transmembrane region profile" evidence="20">
        <location>
            <begin position="1"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome oxidase subunit II copper A binding" evidence="19">
        <location>
            <begin position="92"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="CAD56915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000036591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="229" mass="26526" checksum="FCA043BEFB257F36" modified="2003-03-01" version="1">MYPYSFIQLTDSNTFFGHEIVLFHDFSLASLVFILTAVLLMSKALKKKKYVVLAYKGSNMLEFLWTFFPVFILISIGLPSLYLMYLLEGSFKGDLTFKVVGHQWYWSYEGGDFNDFDMSSYMVPSEDLQEGQFRLLEVDNSMVLPYSTNIRLMITSYDVIHSWTLPSLKMKVDAVPGRLNTMNIFSFQPGSFYGQCSEICGLLHSFMPIHVEFVNWETFLNHVTEESFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>F6PLY6</accession>
    <accession>A0A1W2WEI0</accession>
    <name>F6PLY6_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Exosome component 7</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Ribosomal RNA-processing protein 42</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100184256</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">wild type</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="11">Component of the RNA exosome core complex (Exo-9), composed of EXOSC1, EXOSC2, EXOSC3, EXOSC4, EXOSC5, EXOSC6, EXOSC7, EXOSC8 and EXOSC9; within the complex interacts with EXOSC2 and EXOSC4. The catalytically inactive RNA exosome core complex (Exo-9) associates with the catalytic subunit EXOSC10/RRP6. Exo-9 may associate with DIS3 to form the nucleolar exosome complex, or DIS3L to form the cytoplasmic exosome complex. Exo-9 is formed by a hexameric base ring consisting of the heterodimers EXOSC4-EXOSC9, EXOSC5-EXOSC8 and EXOSC6-EXOSC7, and a cap ring consisting of EXOSC1, EXOSC2 and EXOSC3. The RNA exosome complex associates with cofactors C1D/RRP47, MPHOSPH6/MPP6 and MTREX/MTR4. Interacts with ZC3HAV1. Interacts with DIS3; the interaction is direct.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RNase PH family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01000324">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002127184.1">
        <property type="nucleotide sequence ID" value="XM_002127148.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6PLY6"/>
    <dbReference type="FunCoup" id="F6PLY6">
        <property type="interactions" value="463"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000009867"/>
    <dbReference type="Ensembl" id="ENSCINT00000009867.3">
        <property type="protein sequence ID" value="ENSCINP00000009867.3"/>
        <property type="gene ID" value="ENSCING00000004774.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100184256"/>
    <dbReference type="KEGG" id="cin:100184256"/>
    <dbReference type="GeneTree" id="ENSGT00950000183130"/>
    <dbReference type="HOGENOM" id="CLU_038194_4_2_1"/>
    <dbReference type="InParanoid" id="F6PLY6"/>
    <dbReference type="OMA" id="YNTRIPK"/>
    <dbReference type="OrthoDB" id="272245at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035925">
        <property type="term" value="F:mRNA 3'-UTR AU-rich region binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000467">
        <property type="term" value="P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071028">
        <property type="term" value="P:nuclear mRNA surveillance"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071035">
        <property type="term" value="P:nuclear polyadenylation-dependent rRNA catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071038">
        <property type="term" value="P:TRAMP-dependent tRNA surveillance pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034473">
        <property type="term" value="P:U1 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034476">
        <property type="term" value="P:U5 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd11367">
        <property type="entry name" value="RNase_PH_RRP42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.70:FF:000009">
        <property type="entry name" value="Exosome complex component RRP42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.70">
        <property type="entry name" value="GHMP Kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001247">
        <property type="entry name" value="ExoRNase_PH_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015847">
        <property type="entry name" value="ExoRNase_PH_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036345">
        <property type="entry name" value="ExoRNase_PH_dom2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050590">
        <property type="entry name" value="Exosome_comp_Rrp42_subfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027408">
        <property type="entry name" value="PNPase/RNase_PH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11097:SF8">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT RRP42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11097">
        <property type="entry name" value="EXOSOME COMPLEX EXONUCLEASE RIBOSOMAL RNA PROCESSING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01138">
        <property type="entry name" value="RNase_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03725">
        <property type="entry name" value="RNase_PH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55666">
        <property type="entry name" value="Ribonuclease PH domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0271">Exosome</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="13">
        <location>
            <begin position="32"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="14">
        <location>
            <begin position="197"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00042523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00064326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00083632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF03725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000009867.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="290" mass="31293" checksum="FD4C6E41A04B15EC" modified="2012-04-18" version="2">MAKVMLGESEKWFIIHGIQDDMRNDGRSCEDYRHIEVETGVVSNTHGSSRLRLGSSELLVGVKVELGSPDPKAPNEGLIHFHVDCSPNADPSFQGRGGEDLSANISCILTRIYCNKSSIDLNTLGIIPHKQCWVVHVDVLILECGGNLLDAVSIAVKAALHNTGVPNIQVTDGDEGTQEISLPDDPNDVKFINTVNAPVLVTISKVGSRHVVDASSEEELCCNASLVMGVTKRGRTTGMRKMGGGSLDPESIPEMIETGKNIGRQLHISLDKLLKTEQGMAEDIEKAGYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>F6PMV6</accession>
    <name>F6PMV6_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CUB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">wild type</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01000154">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6PMV6"/>
    <dbReference type="Ensembl" id="ENSCINT00000025317.2">
        <property type="protein sequence ID" value="ENSCINP00000025071.2"/>
        <property type="gene ID" value="ENSCING00000013715.2"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1673159_0_0_1"/>
    <dbReference type="InParanoid" id="F6PMV6"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CUB" evidence="3">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000025071.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    </evidence>
    <sequence length="158" mass="17676" checksum="38DE44D25036119E" modified="2012-04-18" version="2">MVHFSNCMGIIQPKNITNKSIYIQFVYVENSCSCDFLNVYDGYSVHDPFLATYHGAVNPIQLPAVVSSTGSVVLQFNNDDMPFLNKGYTLYYWLDDYPPSDPGDVGSGDEPAENNTSCIDNWCIHKWPEFLKQGRASAALQSLNGMLYIYGGYLFTLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>F6PXA1</accession>
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            <fullName evidence="6">UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit</fullName>
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            <fullName evidence="14">O-linked N-acetylglucosamine transferase 110 kDa subunit</fullName>
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        <name evidence="17" type="primary">LOC100181206</name>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">wild type</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyltransferase 41 family. O-GlcNAc transferase subfamily.</text>
    </comment>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="Ensembl" id="ENSCINT00000013052.3">
        <property type="protein sequence ID" value="ENSCINP00000013052.3"/>
        <property type="gene ID" value="ENSCING00000006241.3"/>
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    <dbReference type="InParanoid" id="F6PXA1"/>
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        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:protein O-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="entry name" value="UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit"/>
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        <property type="entry name" value="UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit"/>
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        <property type="entry name" value="UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit"/>
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        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Tetratricopeptide repeat domain"/>
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        <property type="entry name" value="OGT"/>
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        <property type="entry name" value="OGT/SEC/SPY_C"/>
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        <property type="entry name" value="TPR-like_helical_dom_sf"/>
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        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE--PEPTIDE N-ACETYLGLUCOSAMINYLTRANSFERASE 110 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE--PEPTIDE N-ACETYLGLUCOSAMINYLTRANSFERASE 110 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Glyco_transf_41"/>
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    </dbReference>
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        <property type="entry name" value="TPR_11"/>
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        <property type="entry name" value="TPR_8"/>
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    </dbReference>
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        <property type="match status" value="9"/>
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    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="4"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="11 15" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
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        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="15">
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        </location>
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        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="15">
        <location>
            <begin position="420"/>
            <end position="453"/>
        </location>
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    <feature type="domain" description="O-GlcNAc transferase C-terminal" evidence="16">
        <location>
            <begin position="467"/>
            <end position="1005"/>
        </location>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00029769"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00031470"/>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
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    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF13844"/>
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    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000013052.3"/>
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    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    </evidence>
    <sequence length="1027" mass="114625" checksum="5CA9DC7A8E806B2F" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>F6QEF4</accession>
    <name>F6QEF4_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Matrilin-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100175501</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
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            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
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    <proteinExistence type="predicted"/>
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        <location>
            <begin position="67"/>
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        </location>
    </feature>
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        <location>
            <begin position="131"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="338" mass="37774" checksum="B0BB853B8D6D916D" modified="2012-04-18" version="2">MVWRSGSNYHVIKQCKQRLACANNQLQNTKSSGVSTQCNNDQPNSMCTCCCHDDRCNVGDVTCTQDQTCPPQNQMPHGHITCTDRNQPGSHCTFECTDHEYDVYPAGKNRNHCLSNGSWSESPPCCARPCPPYFLADIVTLYHSSNIHAYEHMVRIAFLSAEQTAIAGDAVKYAGLYYSGEVNMESIVHFNEYNDDKLTMQGLFMQRVIMNNTADRKVNTGAAMEFARTHMFNQSNGVRGEVPRILMVITDENSTDDVLEPAEKLRAEGVLTYAIGLAEDGKILDRDQLMQIAGEDDHLFNPDHDISQVGVGLINEFTVHICADPCVHALHNHEHFAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>F6QJC1</accession>
    <name>F6QJC1_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Fibronectin type-III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="F6QJC1"/>
    <dbReference type="Ensembl" id="ENSCINT00000006424.3">
        <property type="protein sequence ID" value="ENSCINP00000006424.3"/>
        <property type="gene ID" value="ENSCING00000003154.3"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_644872_0_0_1"/>
    <dbReference type="InParanoid" id="F6QJC1"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
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    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
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    <dbReference type="InterPro" id="IPR050991">
        <property type="entry name" value="ECM_Regulatory_Proteins"/>
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        <property type="entry name" value="FN3_dom"/>
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    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
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    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
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    <dbReference type="PANTHER" id="PTHR46708:SF2">
        <property type="entry name" value="FIBRONECTIN TYPE-III DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
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        <property type="match status" value="2"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="1" id="KW-0677">Repeat</keyword>
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        <location>
            <begin position="140"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="3">
        <location>
            <begin position="353"/>
            <end position="426"/>
        </location>
    </feature>
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        <location>
            <begin position="276"/>
            <end position="312"/>
        </location>
    </feature>
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        <location>
            <begin position="57"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000006424.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="426" mass="47655" checksum="9AA659B2A92F8B78" modified="2012-04-18" version="2">MVETQPELRLSYHSPMQAKTSQKVAHFTLKRNIKDFYEELTWVVTKEMRRKSDDDSSTSSSQSSSSSDDSDSPHEVARGSVTFNPGEDTVVVDYPLKQNMRHASQVYRLLMRKQPCDDDRERPLANVELNVINDTGYPGRTRKPFASSVSPCDCSVSWESPAGGGPVDGYQVVAKTKGEPDLMVTVGPDERRTILTGLKPSTDYDIIVYPFNEVGEARPSQPAHVTTQDELKVEFSEKFYSFKASDKVAVIPLKRHFTRGYATLNWNARWCKRHEDNVNSDSESDSEGRARDFASGSITFPDGSEGSEVRIPLQQVPPKKKSFFWINISDPNRKKRLAQMRCSLLDDRAHLVAPKDPKVDVTGPTSMVVTWSPQEAPVHEYMVAYNRVGANAEPHRHTLPSSDHSIAIDDLTPESAYVVVVSAMGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>F6QK43</accession>
    <name>F6QK43_CIOIN</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
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            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">wild type</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
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        <scope>IDENTIFICATION</scope>
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        <source>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
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                <person name="Hino K."/>
                <person name="Imai K.S."/>
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                <person name="Kano S."/>
                <person name="Kobayashi K."/>
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                <person name="Makabe K.W."/>
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                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
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                <person name="Richardson P."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="7">wild type</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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                <consortium name="Ensembl"/>
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        <source>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
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                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="27">wild type</strain>
        </source>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of N-terminal glutamate (and to a lesser extent aspartate) from a peptide.</text>
            <dbReference type="EC" id="3.4.11.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="21 23">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="21 23">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer; disulfide-linked.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 23">Belongs to the peptidase M1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.-" evidence="23"/>
    <dbReference type="EMBL" id="EAAA01000999">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6SLT5"/>
    <dbReference type="STRING" id="7719.ENSCINP00000008091"/>
    <dbReference type="MEROPS" id="M01.003"/>
    <dbReference type="Ensembl" id="ENSCINT00000008091.3">
        <property type="protein sequence ID" value="ENSCINP00000008091.3"/>
        <property type="gene ID" value="ENSCING00000003909.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156946"/>
    <dbReference type="HOGENOM" id="CLU_003705_0_1_1"/>
    <dbReference type="InParanoid" id="F6SLT5"/>
    <dbReference type="OMA" id="RPIKYKI"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004230">
        <property type="term" value="F:glutamyl aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002003">
        <property type="term" value="P:angiotensin maturation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd09601">
        <property type="entry name" value="M1_APN-Q_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.390.10:FF:000001">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.50.20:FF:000001">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1910:FF:000006">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1730:FF:000001">
        <property type="entry name" value="Leucyl-cystinyl aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045357">
        <property type="entry name" value="Aminopeptidase_N-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024571">
        <property type="entry name" value="ERAP1-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034016">
        <property type="entry name" value="M1_APN-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001930">
        <property type="entry name" value="Peptidase_M1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050344">
        <property type="entry name" value="Peptidase_M1_aminopeptidases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533:SF276">
        <property type="entry name" value="GLUTAMYL AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533">
        <property type="entry name" value="PROTEASE M1 ZINC METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11838">
        <property type="entry name" value="ERAP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17900">
        <property type="entry name" value="Peptidase_M1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00756">
        <property type="entry name" value="ALADIPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 23" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="12" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 23" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 21 23" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15 23" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="7 23" id="KW-0645">Protease</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11 21 23" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Aminopeptidase N-like N-terminal" evidence="26">
        <location>
            <begin position="15"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M1 membrane alanine aminopeptidase" evidence="24">
        <location>
            <begin position="247"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="domain" description="ERAP1-like C-terminal" evidence="25">
        <location>
            <begin position="548"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="19">
        <location>
            <position position="320"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <begin position="283"/>
            <end position="287"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="319"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="323"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="342"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="818"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="22">
        <location>
            <position position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="RuleBase" id="RU364040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF01433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="Pfam" id="PF11838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="Pfam" id="PF17900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000008091.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="888" mass="101444" checksum="D2772FEA4F0987B6" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>F6SXA9</accession>
    <name>F6SXA9_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA polymerase subunit gamma-1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Mitochondrial DNA polymerase catalytic subunit</fullName>
        </alternativeName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
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                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
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                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
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                <person name="Kobayashi K."/>
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                <person name="Oishi K."/>
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                <person name="Zhang C."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Ueno K."/>
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                <person name="Wiley G.B."/>
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                <person name="Roe B.A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Wang H.G."/>
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                <person name="Goodstein D.M."/>
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                <person name="Hastings K.E."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
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                <person name="Wiley G.B."/>
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                <person name="Richardson P."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">wild type</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001120">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6UZH2"/>
    <dbReference type="Ensembl" id="ENSCINT00000026515.2">
        <property type="protein sequence ID" value="ENSCINP00000026269.2"/>
        <property type="gene ID" value="ENSCING00000014555.2"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1418078_0_0_1"/>
    <dbReference type="InParanoid" id="F6UZH2"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22750">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 6 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="52"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="87"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="129"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="168"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="8">
        <location>
            <begin position="68"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000026269.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="192" mass="21312" checksum="D77AB883E950FBA0" modified="2012-04-18" version="2">MTSTSTSNSSIYKTTTSSYKLMTSPYTSSNHTNSSLIQGDRHICITTQEVSIVLNIITIIVGVVIVLVNFLVLLVSFKYPSKRFVALNWLICHLAFADLFIGVTIFYWYGIAEVLNVPVSSTEKVGFCGLSGVCIQASIQGLMMIALDRYLLILKQVKYSSYITKQRIFIIIPLMWIIPSVLQIVIPLSGSS</sequence>
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<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
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            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_002127498.5"/>
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    <dbReference type="Ensembl" id="ENSCINT00000028848.2">
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        <property type="gene ID" value="ENSCING00000016549.2"/>
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    <dbReference type="KEGG" id="cin:100181126"/>
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    <dbReference type="InParanoid" id="F6VGA3"/>
    <dbReference type="OMA" id="IQYQMAV"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000028602.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="307" mass="31818" checksum="C4426674BEC3301C" modified="2012-04-18" version="2">MNSQQYIQYQMAVSAGIQGIPAYAPGVTPYLPMANPSLTAGLQGVVPPPPPPVAVSSPEINEIENPSSVTITTTSVAMTEETAEKPAADTIASNDSEKTVDQVELKSPQAALTSTTMTTSAVPNAVVPTPMVLPSTSQPSLIGGVYAGAYIQQAIPQQPAQVPVAQSPYMMAQAGVQYAIPQSSKVMYIPQGYGGVQPSPYVAAGQQPMLVQDHTGKTYILNAPAAHAPLQYQMAQPGLKAPGQQYVVVPNQYYAGAVPTNPAVQQVYGRQAAAAGAATAQSIAPLASYPPGGTVPGAQSPQVFYYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>F6VKH0</accession>
    <name>F6VKH0_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
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    </protein>
    <organism evidence="2 3">
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        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="F6VKH0"/>
    <dbReference type="Ensembl" id="ENSCINT00000027053.2">
        <property type="protein sequence ID" value="ENSCINP00000026807.2"/>
        <property type="gene ID" value="ENSCING00000014949.2"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_638657_0_0_1"/>
    <dbReference type="InParanoid" id="F6VKH0"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="106"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000026807.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="430" mass="48069" checksum="9EC74A55DC8B9A5A" modified="2012-04-18" version="2">MISLQPEPHGDDTSDPYDSVHEDDDLNKLILEANSLKIRGQHDRVEPVETLFPSYKRSHQLNKELRTDLTGAFAKLAADIEPVEVIRDAEPDIMLVCEIPVKNARKRKSGLPEDSLPTSQDPLDGDDEISLAESDFRFPVPPSSVGKMDGLSANEVYPSDYTDFESASESDHTEVESRIEVNPLNFDITPYDTVADQETDWEIPRTKKDDDETNLIVSEDYQICTDLRNRNTFPRRLHANKPVAKERTSLHAMAIALTDPSALSATTESKRRAPSHFVVDRPQILVTRGTTHPDGGYEPQHHEPHFQPRVKSRTKSEGNLVISEPQVVPQPQVIPQPQVIPPAPQIHVSVTPNSDSDATTLGNHGNLRINLLPSEDPNIINLNIRNDVINRDVIPPSHDIIIPQQSEPEISPLQVQPMTEMTAITYRPTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F6VP62</accession>
    <name>F6VP62_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BACK domain-containing protein</fullName>
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            <taxon>Phlebobranchia</taxon>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4">wild type</strain>
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    <reference evidence="4" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
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                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
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                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
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                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
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                <person name="Nomoto H."/>
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                <person name="Oishi K."/>
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                <person name="Sasaki A."/>
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                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
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                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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    <reference evidence="11" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="11">wild type</strain>
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    <reference evidence="11" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="11" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <text evidence="4">Catalyzes two distinct but analogous reactions: the reversible epimerization of UDP-glucose to UDP-galactose and the reversible epimerization of UDP-N-acetylglucosamine to UDP-N-acetylgalactosamine. The reaction with UDP-Gal plays a critical role in the Leloir pathway of galactose catabolism in which galactose is converted to the glycolytic intermediate glucose 6-phosphate. It contributes to the catabolism of dietary galactose and enables the endogenous biosynthesis of both UDP-Gal and UDP-GalNAc when exogenous sources are limited. Both UDP-sugar interconversions are important in the synthesis of glycoproteins and glycolipids.</text>
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            <text>UDP-N-acetyl-alpha-D-glucosamine = UDP-N-acetyl-alpha-D-galactosamine</text>
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            <name>NAD(+)</name>
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    <comment type="pathway">
        <text evidence="5 9">Carbohydrate metabolism; galactose metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homodimer.</text>
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    <comment type="similarity">
        <text evidence="9">Belongs to the NAD(P)-dependent epimerase/dehydratase family.</text>
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                <person name="De Tomaso A."/>
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                <person name="Di Gregorio A."/>
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                <person name="Huang W."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Ueno K."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
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                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
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                <person name="Wiley G.B."/>
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                <person name="Roe B.A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">wild type</strain>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
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                <person name="Di Gregorio A."/>
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                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Required for mitoribosome formation and stability, and mitochondrial translation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uS2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002126843.1">
        <property type="nucleotide sequence ID" value="XM_002126807.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6WCB0"/>
    <dbReference type="FunCoup" id="F6WCB0">
        <property type="interactions" value="48"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000017774"/>
    <dbReference type="Ensembl" id="ENSCINT00000017774.3">
        <property type="protein sequence ID" value="ENSCINP00000017774.3"/>
        <property type="gene ID" value="ENSCING00000008713.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100178663"/>
    <dbReference type="KEGG" id="cin:100178663"/>
    <dbReference type="GeneTree" id="ENSGT00390000017382"/>
    <dbReference type="HOGENOM" id="CLU_040318_3_0_1"/>
    <dbReference type="InParanoid" id="F6WCB0"/>
    <dbReference type="OMA" id="PYIFMEK"/>
    <dbReference type="OrthoDB" id="2320368at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01425">
        <property type="entry name" value="RPS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10490:FF:000026">
        <property type="entry name" value="28S ribosomal protein S2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00291_B">
        <property type="entry name" value="Ribosomal_uS2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001865">
        <property type="entry name" value="Ribosomal_uS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005706">
        <property type="entry name" value="Ribosomal_uS2_bac/mit/plastid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018130">
        <property type="entry name" value="Ribosomal_uS2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023591">
        <property type="entry name" value="Ribosomal_uS2_flav_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12534">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S2 PROKARYOTIC AND ORGANELLAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12534:SF0">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00318">
        <property type="entry name" value="Ribosomal_S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00395">
        <property type="entry name" value="RIBOSOMALS2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52313">
        <property type="entry name" value="Ribosomal protein S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00962">
        <property type="entry name" value="RIBOSOMAL_S2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00059792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00071390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00083109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000017774.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="278" mass="31784" checksum="2545BD5C8439634A" modified="2012-04-18" version="2">MKHCLMLNKAWRGFTRVANTYHSNTCVSQMNTLHKNCRHFSTVLPQQTGKDPSPILHKEKRQLITDPLQHPDFFGIKNLVTMKKLFFSRVYFGHKKGSRNEHMQPYLYGNRLGVDIFDLDQTLEHMHRALNFVAHIAYLKGIILFIMRSQQHGHAVELAAKACGEYAHTREWQQGLLLNMGQKHRSVRLPDICIFLSLQDVMNQEHPAVAECGKLKIATVGIVDSNVNPTMITYPVPGNDDSPESVGLYLQLFQLAIQSGKQKRIEADQQKQDVSQLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>F6WHM9</accession>
    <name>F6WHM9_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01000627">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6WHM9"/>
    <dbReference type="STRING" id="7719.ENSCINP00000015989"/>
    <dbReference type="Ensembl" id="ENSCINT00000015989.3">
        <property type="protein sequence ID" value="ENSCINP00000015989.3"/>
        <property type="gene ID" value="ENSCING00000007812.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000171221"/>
    <dbReference type="HOGENOM" id="CLU_046864_0_0_1"/>
    <dbReference type="InParanoid" id="F6WHM9"/>
    <dbReference type="OMA" id="REGTERY"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051746">
        <property type="entry name" value="Kelch_domain_containing_8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46260">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46260:SF3">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000015989.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="338" mass="36883" checksum="B2705C9ACC579069" modified="2012-04-18" version="2">NSTMSLANGTWTSIGSLSGPRVYMSVVICDGSIYVVGGCDEMGQPVNFVEVYSSEEDTWTKLKSMHTKRAAPIVTAVEGRIVAIGGVGVAQAPVDAVEVFSVAENKWKKLTPLSEPLMGMAHFLRDNKVHIFGGMGIDTNPRDHFKCLVVGASAGGEKWQAFTPMPTARYAAHAFYKNSKAYVLGGRVGKLPVAVFEVYDFDSRIWVSYPEILSKRVFPCYAMTDKHIVSLGGLKQTAQQGFCDNCEIYSTEQNDKGEWQTNKRMSIPTKRGDFTAKAIDNQVLVAGGLGNNGKPLSAVELFDPETKKWKRLADLPRAHSTCASVVHQGKFYLIAGVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>F6WIH5</accession>
    <name>F6WIH5_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Sulfiredoxin</fullName>
            <ecNumber evidence="10">1.8.98.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">wild type</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Contributes to oxidative stress resistance by reducing cysteine-sulfinic acid formed under exposure to oxidants in the peroxiredoxins PRDX1, PRDX2, PRDX3 and PRDX4. Does not act on PRDX5 or PRDX6. May catalyze the reduction in a multi-step process by acting both as a specific phosphotransferase and a thioltransferase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 10">
            <text>S-hydroxy-S-oxy-L-cysteinyl-[peroxiredoxin] + [protein]-dithiol + ATP = S-hydroxy-L-cysteinyl-[peroxiredoxin] + [protein]-disulfide + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17545"/>
            <dbReference type="Rhea" id="RHEA-COMP:10593"/>
            <dbReference type="Rhea" id="RHEA-COMP:10594"/>
            <dbReference type="Rhea" id="RHEA-COMP:13681"/>
            <dbReference type="Rhea" id="RHEA-COMP:17976"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:61973"/>
            <dbReference type="ChEBI" id="CHEBI:61974"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="1.8.98.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the sulfiredoxin family.</text>
    </comment>
    <dbReference type="EC" id="1.8.98.2" evidence="10"/>
    <dbReference type="EMBL" id="EAAA01002870">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6WIH5"/>
    <dbReference type="FunCoup" id="F6WIH5">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000003685"/>
    <dbReference type="Ensembl" id="ENSCINT00000003685.3">
        <property type="protein sequence ID" value="ENSCINP00000003685.3"/>
        <property type="gene ID" value="ENSCING00000001830.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000007832"/>
    <dbReference type="HOGENOM" id="CLU_124532_1_1_1"/>
    <dbReference type="InParanoid" id="F6WIH5"/>
    <dbReference type="OMA" id="SQIRRPI"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032542">
        <property type="term" value="F:sulfiredoxin activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd16395">
        <property type="entry name" value="Srx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1530.10:FF:000001">
        <property type="entry name" value="Sulfiredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1530.10">
        <property type="entry name" value="Conserved hypothetical protein from pyrococcus furiosus pfu- 392566-001, ParB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036086">
        <property type="entry name" value="ParB/Sulfiredoxin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003115">
        <property type="entry name" value="ParB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016692">
        <property type="entry name" value="Sulfiredoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21348">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21348:SF2">
        <property type="entry name" value="SULFIREDOXIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02195">
        <property type="entry name" value="ParB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017267">
        <property type="entry name" value="Sulfiredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110849">
        <property type="entry name" value="ParB/Sulfiredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="4 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0488">Methylation</keyword>
    <keyword evidence="3 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ParB-like N-terminal" evidence="13">
        <location>
            <begin position="20"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="80"/>
            <end position="83"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" description="Interchain" evidence="12">
        <location>
            <position position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00059800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR017267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR017267-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR017267-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000003685.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="119" mass="13639" checksum="21E4FFEF00AE842E" modified="2012-04-18" version="2">MANKKTEDTSTSIHSGHIQDVHTMPMKYLIRPFPSELDQNKVKSLMETLEDKELESSVPPIDVLWIKGREGGDYFYSFGGCHRYEAHKVLSRENIKVKLVRGNQEMLRTYLGASTPNLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>F6WM77</accession>
    <accession>A0A1W2W417</accession>
    <name>F6WM77_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized LOC100186174</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100186174</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_002123766.1">
        <property type="nucleotide sequence ID" value="XM_002123730.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6WM77"/>
    <dbReference type="Ensembl" id="ENSCINT00000022486.2">
        <property type="protein sequence ID" value="ENSCINP00000022240.2"/>
        <property type="gene ID" value="ENSCING00000011692.2"/>
    </dbReference>
    <dbReference type="GeneID" id="100186174"/>
    <dbReference type="KEGG" id="cin:100186174"/>
    <dbReference type="HOGENOM" id="CLU_764949_0_0_1"/>
    <dbReference type="InParanoid" id="F6WM77"/>
    <dbReference type="OrthoDB" id="6375837at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051495">
        <property type="entry name" value="Epithelial_Barrier/Signaling"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003886">
        <property type="entry name" value="NIDO_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13802">
        <property type="entry name" value="MUCIN 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13802:SF52">
        <property type="entry name" value="MUCIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06119">
        <property type="entry name" value="NIDO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00539">
        <property type="entry name" value="NIDO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="domain" description="NIDO" evidence="3">
        <location>
            <begin position="109"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000022240.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="362" mass="41246" checksum="816C344DF56BA0BA" modified="2012-04-18" version="2">MQMAGYFADTARSLYMVTVLHALIAISVCQKLLPFGSKYGDSRLSLEDWSRGYVKVNLTSSINVYGRAMFNELYINTVGAISFGQPVLTHHLDQIQNDVIILAPFLLATEYGDLTFRKLFFREPQSEEEKIEIQRTFRVAIGNSEFLAQRALVVTFIPMYRTNTTQSRELINCFQITLATDGDKTYVRYAYDHLQWIKSEQYYARAGQFVLTGGVLSCSSELPGSGTEEVAGLWAKSNVGVQGNFYWRIDLPLQCSSFGSPCGDINPPPSEANVYRAGNFIGTVYKDQWSFYVFYFCKAGYSTQDNRVSIKIECRYDPDYYQYEWRELSSVEFCTRVTELPSKFLQRIVSTPQVNETTTTVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>F6X3Y1</accession>
    <accession>A0A1W2WAQ4</accession>
    <name>F6X3Y1_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Volume-regulated anion channel subunit LRRC8B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100185148</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">wild type</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="EAAA01000626">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002127699.1">
        <property type="nucleotide sequence ID" value="XM_002127663.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6X3Y1"/>
    <dbReference type="FunCoup" id="F6X3Y1">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000015962"/>
    <dbReference type="TCDB" id="1.A.25.3.2">
        <property type="family name" value="the gap junction-forming innexin (innexin) family"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSCINT00000015962.3">
        <property type="protein sequence ID" value="ENSCINP00000015962.3"/>
        <property type="gene ID" value="ENSCING00000007791.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100185148"/>
    <dbReference type="KEGG" id="cin:100185148"/>
    <dbReference type="GeneTree" id="ENSGT00440000036553"/>
    <dbReference type="HOGENOM" id="CLU_044855_0_0_1"/>
    <dbReference type="InParanoid" id="F6X3Y1"/>
    <dbReference type="OMA" id="WAFKTTA"/>
    <dbReference type="OrthoDB" id="10588275at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021040">
        <property type="entry name" value="LRRC8_Pannexin-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12534">
        <property type="entry name" value="Pannexin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="136"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="262"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="302"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="domain" description="LRRC8 pannexin-like TM region" evidence="9">
        <location>
            <begin position="10"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="455"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="461"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF12534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000015962.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="480" mass="55113" checksum="D9591C6916A8470F" modified="2012-04-18" version="2">MASSWAFKTTASLIKPTREALLDYCAYILLVIAIIGTTRIIIGRSELQQLCPRKTFIQSNTSQPLTHEFNKTGDIVLTKPNLDKETRQFVLQSNLKVHLVKDLKVAEGEDDISVHKQKQDFNSSVLYFCLKHYQAYLPYFLLAESAVLLLIGILWVKMPSVQLLIKEFASILHECRQSTWYVSVADVLQSESTIPVTQSLFTLMKSDAVNMEMILLLETLFRPNSAYLNKSQARRLYERVQKFCIENENSVVLLDSYISKSIVQLIAWMLFFFINMVLFVKFEFYSTCGWSEVCEHDTFLRIIWYMAQLLVLVYGIFNCSALKWINDKAVYINRPRRNFLCCEDDNDDSSSKKRKFVKCRPFKVGQDQTVVHNDLALLLHLASSSNHKTITYFTIFLFDQWKEKFSQPVEDVHIESPVKQKLVKQFPARKSNSSSHPLLTKSSSYGKVSFFIESPQDEATDSPSSSSKTSGDVSDRISST</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>F6XRG3</accession>
    <name>F6XRG3_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Prohibitin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">wild type</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Protein with pleiotropic attributes mediated in a cell-compartment- and tissue-specific manner, which include the plasma membrane-associated cell signaling functions, mitochondrial chaperone, and transcriptional co-regulator of transcription factors and sex steroid hormones in the nucleus.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the prohibitin family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002256">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6XRG3"/>
    <dbReference type="STRING" id="7719.ENSCINP00000005164"/>
    <dbReference type="Ensembl" id="ENSCINT00000005164.3">
        <property type="protein sequence ID" value="ENSCINP00000005164.3"/>
        <property type="gene ID" value="ENSCING00000002532.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183070"/>
    <dbReference type="HOGENOM" id="CLU_047969_5_1_1"/>
    <dbReference type="InParanoid" id="F6XRG3"/>
    <dbReference type="OMA" id="ACFESIG"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd03401">
        <property type="entry name" value="SPFH_prohibitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000163">
        <property type="entry name" value="Prohibitin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23222">
        <property type="entry name" value="PROHIBITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23222:SF1">
        <property type="entry name" value="PROHIBITIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00679">
        <property type="entry name" value="PROHIBITIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00244">
        <property type="entry name" value="PHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117892">
        <property type="entry name" value="Band 7/SPFH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Band 7" evidence="8">
        <location>
            <begin position="33"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Chapman J."/>
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                <person name="De Tomaso A."/>
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                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
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                <person name="Hastings K.E."/>
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                <person name="Azumi K."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
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            <authorList>
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        <scope>IDENTIFICATION</scope>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <source>
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<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">wild type</strain>
        </source>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Mediates responses to nitric oxide (NO) by catalyzing the biosynthesis of the signaling molecule cGMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>GTP = 3',5'-cyclic GMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:13665"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:57746"/>
            <dbReference type="EC" id="4.6.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.2" evidence="4"/>
    <dbReference type="EMBL" id="EAAA01001463">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6YD09"/>
    <dbReference type="FunCoup" id="F6YD09">
        <property type="interactions" value="9"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000017414"/>
    <dbReference type="Ensembl" id="ENSCINT00000017414.3">
        <property type="protein sequence ID" value="ENSCINP00000017414.3"/>
        <property type="gene ID" value="ENSCING00000008538.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157483"/>
    <dbReference type="HOGENOM" id="CLU_011614_4_0_1"/>
    <dbReference type="InParanoid" id="F6YD09"/>
    <dbReference type="OMA" id="SHARCIG"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008074">
        <property type="term" value="C:guanylate cyclase complex, soluble"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004383">
        <property type="term" value="F:guanylate cyclase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006182">
        <property type="term" value="P:cGMP biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019934">
        <property type="term" value="P:cGMP-mediated signaling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070482">
        <property type="term" value="P:response to oxygen levels"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1520.10:FF:000012">
        <property type="entry name" value="Chromosome undetermined SCAF14725, whole genome shotgun sequence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.260:FF:000002">
        <property type="entry name" value="guanylate cyclase soluble subunit alpha-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1230:FF:000005">
        <property type="entry name" value="Guanylate cyclase soluble subunit beta-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1520.10">
        <property type="entry name" value="H-NOX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.260">
        <property type="entry name" value="Haem NO binding associated domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018297">
        <property type="entry name" value="A/G_cyclase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038158">
        <property type="entry name" value="H-NOX_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011644">
        <property type="entry name" value="Heme_NO-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011645">
        <property type="entry name" value="HNOB_dom_associated"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042463">
        <property type="entry name" value="HNOB_dom_associated_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024096">
        <property type="entry name" value="NO_sig/Golgi_transp_ligand-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45655:SF2">
        <property type="entry name" value="GUANYLATE CYCLASE SOLUBLE SUBUNIT BETA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45655">
        <property type="entry name" value="GUANYLATE CYCLASE SOLUBLE SUBUNIT BETA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07700">
        <property type="entry name" value="HNOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07701">
        <property type="entry name" value="HNOBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111126">
        <property type="entry name" value="Ligand-binding domain in the NO signalling and Golgi transport"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00452">
        <property type="entry name" value="GUANYLATE_CYCLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0141">cGMP biosynthesis</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0349">Heme</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="11 17" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Guanylate cyclase" evidence="18">
        <location>
            <begin position="415"/>
            <end position="548"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00037442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00039698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00041698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00043208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000017414.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="608" mass="68589" checksum="50EEE176727A4982" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>F6YJ10</accession>
    <name>F6YJ10_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mediator of RNA polymerase II transcription subunit 24</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Mediator complex subunit 24</fullName>
        </alternativeName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">wild type</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Mediator complex subunit 24 family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001387">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="F6YJ10"/>
    <dbReference type="FunCoup" id="F6YJ10">
        <property type="interactions" value="19"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000015307"/>
    <dbReference type="Ensembl" id="ENSCINT00000015307.3">
        <property type="protein sequence ID" value="ENSCINP00000015307.3"/>
        <property type="gene ID" value="ENSCING00000007454.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000016438"/>
    <dbReference type="HOGENOM" id="CLU_007484_0_0_1"/>
    <dbReference type="InParanoid" id="F6YJ10"/>
    <dbReference type="OMA" id="RWSDSQW"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060261">
        <property type="term" value="P:positive regulation of transcription initiation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Ogasawara M."/>
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                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
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                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">wild type</strain>
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    <dbReference type="Ensembl" id="ENSCINT00000026362.2">
        <property type="protein sequence ID" value="ENSCINP00000026116.2"/>
        <property type="gene ID" value="ENSCING00000014436.2"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_848718_0_0_1"/>
    <dbReference type="InParanoid" id="F6YUH2"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="154"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="246"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="247"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="275"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000026116.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="328" mass="36209" checksum="833058692A67BE5B" modified="2012-04-18" version="2">PTFHNDRPPLPPPADDNRYESVGSLSDADLVAPAYATVAEDEDGSTAKLQVTNLPKDYYVSNEEIHRAELSETSHSDAYSTPRNKAKKKNQLQVAKDNSKGSELDIPLVGTDGYLCLNSNERLDATLPNKRYVSVNSASQEDIARHLKQNKAVPSPGITNVTSSLPDLLDKPEPTAQSNPVRFRRKQKLPPTIPAKSATTISNQNEDDSYNKPRPSVPDIFIQCAYASGEDISNVRNRLQKLADKAIQNQQEESESRSLLHSGATSPMPDEELPYYDSPRPSIDSSRDSLLASPGKEFYDAPRPSLPAIFPEDVTRMVNASNPRHFNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>F6YWD0</accession>
    <name>F6YWD0_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Prefoldin subunit 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">wild type</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Heterohexamer of two PFD-alpha type and four PFD-beta type subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the prefoldin subunit beta family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002524">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6YWD0"/>
    <dbReference type="FunCoup" id="F6YWD0">
        <property type="interactions" value="928"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000000844"/>
    <dbReference type="Ensembl" id="ENSCINT00000000844.3">
        <property type="protein sequence ID" value="ENSCINP00000000844.3"/>
        <property type="gene ID" value="ENSCING00000011827.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000009786"/>
    <dbReference type="HOGENOM" id="CLU_122140_2_0_1"/>
    <dbReference type="InParanoid" id="F6YWD0"/>
    <dbReference type="OMA" id="REMIQQK"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044183">
        <property type="term" value="F:protein folding chaperone"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd23164">
        <property type="entry name" value="Prefoldin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002777">
        <property type="entry name" value="PFD_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20903:SF0">
        <property type="entry name" value="PREFOLDIN SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20903">
        <property type="entry name" value="PREFOLDIN SUBUNIT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01920">
        <property type="entry name" value="Prefoldin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="77"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00039325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000000844.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="121" mass="13984" checksum="CC1B4F34B7054A15" modified="2012-04-18" version="2">AKMAVDMELRKAFSDLQMKMVDTQNQMRISDGQINAMMVEKKKTELAISEVSSVPEDAKMYESVGRMFVLEPKATVMKNLEAQHKLTDTKIKELEAKKLYLEKSVKSSEENLREMVLQRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>F6YX22</accession>
    <name>F6YX22_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">diphosphoinositol-polyphosphate diphosphatase</fullName>
            <ecNumber evidence="4">3.6.1.52</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">wild type</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>diphospho-myo-inositol polyphosphate + H2O = myo-inositol polyphosphate + phosphate.</text>
            <dbReference type="EC" id="3.6.1.52"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Nudix hydrolase family. DIPP subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.52" evidence="4"/>
    <dbReference type="EMBL" id="EAAA01002584">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6YX22"/>
    <dbReference type="FunCoup" id="F6YX22">
        <property type="interactions" value="45"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000024551"/>
    <dbReference type="Ensembl" id="ENSCINT00000024797.2">
        <property type="protein sequence ID" value="ENSCINP00000024551.2"/>
        <property type="gene ID" value="ENSCING00000013351.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157882"/>
    <dbReference type="HOGENOM" id="CLU_037162_1_0_1"/>
    <dbReference type="InParanoid" id="F6YX22"/>
    <dbReference type="OMA" id="PDEMQLN"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    </dbReference>
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        <property type="term" value="P:diphosphoinositol polyphosphate metabolic process"/>
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    </dbReference>
    <dbReference type="CDD" id="cd04666">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DDP-like_NUDIX"/>
    </dbReference>
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        <property type="entry name" value="Nudix_hydrolase"/>
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        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12629">
        <property type="entry name" value="DIPHOSPHOINOSITOL POLYPHOSPHATE PHOSPHOHYDROLASE"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR12629:SF0">
        <property type="entry name" value="DIPHOSPHOINOSITOL-POLYPHOSPHATE DIPHOSPHATASE"/>
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    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
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        <property type="entry name" value="NUDIXFAMILY"/>
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    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="11">
        <location>
            <begin position="16"/>
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        <source>
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        <source>
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            <dbReference type="ARBA" id="ARBA00033994"/>
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    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU003476"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51462"/>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000024551.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    </evidence>
    <sequence length="145" mass="16703" checksum="87C15A0C7C551C21" modified="2012-04-18" version="2">KMKIKIDQIRTYDDAGYRQRAACLCFRSECESEILLVSSSRFHDLWIVPGGGLEPGEDPATTAVREVHEEAGVVGQLGRLIDVFENKERNTKTYVYVLIVQQLDEEYDDAKGIGRIRRWFTIPEANKILSQHKPVQMEYIEKLCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>F6YXJ6</accession>
    <accession>A0A1W2WGE1</accession>
    <name>F6YXJ6_CIOIN</name>
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        <submittedName>
            <fullName evidence="5">Uncharacterized LOC100176574</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100176574</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">wild type</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eIF4E-binding protein family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01000397">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002128651.1">
        <property type="nucleotide sequence ID" value="XM_002128615.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6YXJ6"/>
    <dbReference type="Ensembl" id="ENSCINT00000012563.3">
        <property type="protein sequence ID" value="ENSCINP00000012563.3"/>
        <property type="gene ID" value="ENSCING00000006091.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100176574"/>
    <dbReference type="KEGG" id="cin:100176574"/>
    <dbReference type="GeneTree" id="ENSGT00390000015666"/>
    <dbReference type="HOGENOM" id="CLU_1531987_0_0_1"/>
    <dbReference type="InParanoid" id="F6YXJ6"/>
    <dbReference type="OMA" id="CDAFNSC"/>
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        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008190">
        <property type="term" value="F:eukaryotic initiation factor 4E binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030371">
        <property type="term" value="F:translation repressor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045947">
        <property type="term" value="P:negative regulation of translational initiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008606">
        <property type="entry name" value="EIF4EBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12669">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12669:SF12">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05456">
        <property type="entry name" value="eIF_4EBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0652">Protein synthesis inhibitor</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0810">Translation regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="111"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="114"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="141"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000012563.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="175" mass="19691" checksum="8097C7A9CB4458D8" modified="2011-07-27" version="1">MPPVTSNIVLRVYESQPKVEKKKLRSPKRTRKSPRNNRKNEQDLKLDTKEICGNRLVYTRDSLMAFRDSPTSRVAPSLPIIPGVTAPREPKEMPESLKLKLQKKGIIIKENVLPPTSQNGSLTSQVDSVTSQKDRSKQQKKPLTSQNTSHTLKLCSSDVTKPLCDAFNSCMLHVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>F6Z0A0</accession>
    <name>F6Z0A0_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glucose-methanol-choline oxidoreductase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GMC oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01000619">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6Z0A0"/>
    <dbReference type="STRING" id="7719.ENSCINP00000015878"/>
    <dbReference type="Ensembl" id="ENSCINT00000015878.3">
        <property type="protein sequence ID" value="ENSCINP00000015878.3"/>
        <property type="gene ID" value="ENSCING00000007739.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165117"/>
    <dbReference type="HOGENOM" id="CLU_002865_0_1_1"/>
    <dbReference type="InParanoid" id="F6Z0A0"/>
    <dbReference type="OMA" id="PHANIEY"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008812">
        <property type="term" value="F:choline dehydrogenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.560.10">
        <property type="entry name" value="Glucose Oxidase, domain 3"/>
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    </dbReference>
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        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012132">
        <property type="entry name" value="GMC_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007867">
        <property type="entry name" value="GMC_OxRtase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552:SF147">
        <property type="entry name" value="CHOLINE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552">
        <property type="entry name" value="GLUCOSE-METHANOL-CHOLINE GMC OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05199">
        <property type="entry name" value="GMC_oxred_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucose-methanol-choline oxidoreductase C-terminal" evidence="2">
        <location>
            <begin position="150"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000015878.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="304" mass="33692" checksum="47532F5441FD09C8" modified="2012-04-18" version="2">MLSGVGPKQHLKDLGINAIADLPGVGSNLQDHVMVPAPFYATKLPPRSSLDMYTPLLGILPYIFTSSGPLLSSGGVEANAFIRSHLAKEGRPDIQLIVQSARWDFGMTMNMILDLLNLDAEHMKRIEKWRVTQNAETTAHFLIQTGLVRPHSVGTIRLKSSNYKDHPLIDPQYLTDKRDVEILIAAMRKNEALEQTEAFKSVDAKLEFGYYGCGNETSPRSDKFYECVIRLITLTIYHPVGTAKIGSKDDVMAVVDPRLKVYKVDGLRVADASVMPSITSANTQAPCYMIGEKAADMIKEDWVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F6Z1W3</accession>
    <name>F6Z1W3_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">wild type</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002475">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="EAAA01002476">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6Z1W3"/>
    <dbReference type="Ensembl" id="ENSCINT00000022734.2">
        <property type="protein sequence ID" value="ENSCINP00000022488.2"/>
        <property type="gene ID" value="ENSCING00000011878.2"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1874715_0_0_1"/>
    <dbReference type="InParanoid" id="F6Z1W3"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050479">
        <property type="entry name" value="CYP11_CYP27_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24279">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24279:SF120">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0349">Heme</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000022488.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="136" mass="15861" checksum="5BE3AD8F12C83BFD" modified="2012-04-18" version="2">MHNKIILAFSKPQVFSTLPDDNEVLLRSEEKISHREPVEFVVSARKLLGWSIGLPFDVGEDWYKLRKVVNQHFLKNSIVWSHSKQQHEVAEEFVDYIGQNLDENNEVPHFQDLLQKWALESTAVFCFGVRLGRNLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>F6Z4E6</accession>
    <name>F6Z4E6_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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    <reference evidence="17" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
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            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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    <reference evidence="17" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
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    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
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                <person name="Huang W."/>
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                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <source>
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</entry>
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    <accession>F6ZD98</accession>
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        <name type="scientific">Ciona intestinalis</name>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="196" mass="21374" checksum="96B3F13278A24392" modified="2012-04-18" version="2">PSTAPPSTVPPSTAPPSTVPPSTVPPSTAPPSTVPPSTVPPSTVPPSTAPPSTAPPSTAPPSTAPPSSPCLRHPLAYVWAQQKRRPWVGTSSSFVSALLTSINNQVLSSIWNRPNSWIPGQPTIYVSTSTTRRRRWGWRWRIPLPWHGENWFDEDKIKNTLESDKEESHELEELDDEVTDFDDVANTDDVTANDLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>F6ZM33</accession>
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        <recommendedName>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
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                <person name="Chapman J."/>
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                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
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                <person name="Gelpke M."/>
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                <person name="Harafuji N."/>
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                <person name="Satake M."/>
                <person name="Terry A."/>
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                <person name="Awazu S."/>
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                <person name="Mochizuki Y."/>
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                <person name="Takatori N."/>
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                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
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                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
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                <person name="Doggett N."/>
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                <person name="Hawkins T."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="1">Belongs to the IAP family.</text>
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        <property type="gene ID" value="ENSCING00000005071.3"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="F:cysteine-type endopeptidase inhibitor activity involved in apoptotic process"/>
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        <property type="term" value="F:ubiquitin protein ligase activity"/>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:negative regulation of apoptotic process"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0031398">
        <property type="term" value="P:positive regulation of protein ubiquitination"/>
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    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="BIR"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Baculoviral IAP repeat containing 7"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="E3 ubiquitin-protein ligase XIAP"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="1.10.1170.10">
        <property type="entry name" value="Inhibitor Of Apoptosis Protein (2mihbC-IAP-1), Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001370">
        <property type="entry name" value="BIR_rpt"/>
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        <property type="entry name" value="IAP"/>
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    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
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    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10044:SF139">
        <property type="entry name" value="DEATH-ASSOCIATED INHIBITOR OF APOPTOSIS 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10044">
        <property type="entry name" value="INHIBITOR OF APOPTOSIS"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00653">
        <property type="entry name" value="BIR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="BIR"/>
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        <property type="entry name" value="RING"/>
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    </dbReference>
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        <property type="entry name" value="Inhibitor of apoptosis (IAP) repeat"/>
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        <property type="entry name" value="BIR_REPEAT_2"/>
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        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="7">
        <location>
            <begin position="283"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000010449.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="330" mass="37910" checksum="E77DA9697DA0409D" modified="2012-04-18" version="2">MDIRCDGLRVVHNDGRLVPSATNSERELYTRPGDPYYESYRLATFTNYPNQNINVRQVARAGFYFISYQDQVKCFSCGQICEDLQQYDDMSLPQWHLPDCEHLHSLQPNRNNHGVCILGLPRQPTTITINQPRIRSTFTIHEHSNIDLFPCNRPESPHMRSVEQRLQTFTRSNWPAHRVRASILDISRAGLFYLGNSDRTKCWYCNGGLQNWEPNDDPWYEHAKWFPECEFLLQQKGIEYVYRVSSSTTHTTSSTRISAPRSTVVSGPHLSNESVKLEQSRMCKVCHRNQANMVLLPCGHVACCTTCGNDVTNCPVCLADITDRVRSYIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>F6ZML6</accession>
    <name>F6ZML6_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">wild type</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the THOC3 family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002118">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6ZML6"/>
    <dbReference type="STRING" id="7719.ENSCINP00000006964"/>
    <dbReference type="Ensembl" id="ENSCINT00000006964.3">
        <property type="protein sequence ID" value="ENSCINP00000006964.3"/>
        <property type="gene ID" value="ENSCING00000003396.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166119"/>
    <dbReference type="HOGENOM" id="CLU_1885015_0_0_1"/>
    <dbReference type="InParanoid" id="F6ZML6"/>
    <dbReference type="OMA" id="NGHTANC"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:004266">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040132">
        <property type="entry name" value="Tex1/THOC3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22839:SF0">
        <property type="entry name" value="THO COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22839">
        <property type="entry name" value="THO COMPLEX SUBUNIT 3 THO3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="7"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00046343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000006964.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="135" mass="14873" checksum="6FF2D65B7788A395" modified="2012-04-18" version="2">MKLIQSLNGHTANCICIEFDSTGKYFATGSADALVNLWDTNELICVRTFSRLDWPVRTLSFSSNGQLLASASEDLFIDISCVATGEKVCEVPCDSPTFTLAWHPKHKLLAYACDDKDKYNRDAGSVKLFGLPTES</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>F7A1Y9</accession>
    <name>F7A1Y9_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transmembrane 9 superfamily member</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
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            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
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<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
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    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
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                <person name="Sasaki A."/>
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                <person name="Shin-i T."/>
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                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
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                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="7">wild type</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
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<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
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                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
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                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="F7APE7"/>
    <dbReference type="FunCoup" id="F7APE7">
        <property type="interactions" value="59"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000027245"/>
    <dbReference type="Ensembl" id="ENSCINT00000027491.2">
        <property type="protein sequence ID" value="ENSCINP00000027245.2"/>
        <property type="gene ID" value="ENSCING00000015330.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154014"/>
    <dbReference type="HOGENOM" id="CLU_000288_63_0_1"/>
    <dbReference type="InParanoid" id="F7APE7"/>
    <dbReference type="OMA" id="EPFEARY"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043065">
        <property type="term" value="P:positive regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000175">
        <property type="entry name" value="Serine/threonine-protein kinase 17B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24342:SF12">
        <property type="entry name" value="DEATH-ASSOCIATED PROTEIN KINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24342">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000654">
        <property type="entry name" value="Integrin-linked_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 11 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 12" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="31"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00060827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000027245.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="290" mass="32903" checksum="92349CD3469B8E7B" modified="2012-04-18" version="2">LSMPFSSNPRRSGSLTDIRTRIITEPWQNFFNIEDTIGRGKFAVVKKCVEKSSGRRFAAKCIRKRRHCRDCTPDIFHEIAVLEISTHHPHLINLYKVYETTTEVTLILEYAAGGEIFDHCIGVKDPFNETTTRRLLQQILEAVDFLHSNNIVHLDLKPQNILLTEGGVDGDIKLVDFGLSKILAQEIEIREILGTPDYVAPEVLNFEPISTLTDIWSLGVVCYVMLTGVSPFLGDNKNETLMNVTTGVLDFPDDLFKSKNAACKDLMTKMLQRDPSMRINARECLQHPWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>F7ASH4</accession>
    <name>F7ASH4_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Arginyl-tRNA--protein transferase 1</fullName>
            <shortName evidence="5">Arginyltransferase 1</shortName>
            <shortName evidence="5">R-transferase 1</shortName>
            <ecNumber evidence="5">2.3.2.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Arginine-tRNA--protein transferase 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in the post-translational conjugation of arginine to the N-terminal aspartate or glutamate of a protein. This arginylation is required for degradation of the protein via the ubiquitin pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>an N-terminal L-alpha-aminoacyl-[protein] + L-arginyl-tRNA(Arg) = an N-terminal L-arginyl-L-aminoacyl-[protein] + tRNA(Arg) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10208"/>
            <dbReference type="Rhea" id="RHEA-COMP:9658"/>
            <dbReference type="Rhea" id="RHEA-COMP:9673"/>
            <dbReference type="Rhea" id="RHEA-COMP:10636"/>
            <dbReference type="Rhea" id="RHEA-COMP:10638"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78513"/>
            <dbReference type="ChEBI" id="CHEBI:78597"/>
            <dbReference type="ChEBI" id="CHEBI:83562"/>
            <dbReference type="EC" id="2.3.2.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the R-transferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.8" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="F7ASH4"/>
    <dbReference type="FunCoup" id="F7ASH4">
        <property type="interactions" value="788"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000001744"/>
    <dbReference type="Ensembl" id="ENSCINT00000001744.3">
        <property type="protein sequence ID" value="ENSCINP00000001744.3"/>
        <property type="gene ID" value="ENSCING00000000962.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00500000044926"/>
    <dbReference type="HOGENOM" id="CLU_020349_1_1_1"/>
    <dbReference type="InParanoid" id="F7ASH4"/>
    <dbReference type="OMA" id="SDRMVYS"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004057">
        <property type="term" value="F:arginyl-tRNA--protein transferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010498">
        <property type="term" value="P:proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017137">
        <property type="entry name" value="Arg-tRNA-P_Trfase_1_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030700">
        <property type="entry name" value="N-end_Aminoacyl_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007472">
        <property type="entry name" value="N-end_Aminoacyl_Trfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007471">
        <property type="entry name" value="N-end_Aminoacyl_Trfase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21367">
        <property type="entry name" value="ARGININE-TRNA-PROTEIN TRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21367:SF1">
        <property type="entry name" value="ARGINYL-TRNA--PROTEIN TRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04377">
        <property type="entry name" value="ATE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04376">
        <property type="entry name" value="ATE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037207">
        <property type="entry name" value="ATE1_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="N-end aminoacyl transferase N-terminal" evidence="6">
        <location>
            <begin position="12"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="N-end rule aminoacyl transferase C-terminal" evidence="7">
        <location>
            <begin position="272"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR037207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000001744.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="505" mass="58126" checksum="5E836CFC550C7D1C" modified="2012-04-18" version="2">SIVEFFDENDGYKCGYCKSKTSAFSQGMWAHCMNTEDYQALIDRGWRRSGSYCYKPTMHKTCCPQYTIRCDANAFKISKSKKKVAKRMRQFLLNGDKWKLHCDKNNNGETSMSVDEQPDSEHWAPATKFQPTNLLKEPTSSQFTCATQELPKEPNIGKCLYTVYIIYTATFMQCTTLFILTVVGADPSKPPCKKAKQIRLERRKNKLLQQGQHNILPTGKNPKVKCKQQQQAKSLNEFLPHLDSPVDAKHGLEVRLVRSSPPSVEFESSKVESHNLYTKYQTTIHKDKREDCNMKQWMRFLVDSPLNPGTEGPAHGYGSYHNQYWLDGKLVAVGVVDILPSCVSSVYLYYDPDYGFLSFGSYSAISEISFVQRLSITHPNIKYYYLGFYIQSCPKMRYKGAYHPSYLLCPESYEWVPVEKCEAALKEKPYARLNVLNDAVDNDGIVTSLNTVRCLYKHTIILYGKYKMHRNREGNRTAENEDPRVREYAQLVGRSCAERMLLYRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>F7AVB6</accession>
    <name>F7AVB6_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Glutathione reductase</fullName>
            <ecNumber evidence="18">1.8.1.7</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">wild type</strain>
        </source>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Catalyzes the reduction of glutathione disulfide (GSSG) to reduced glutathione (GSH). Constitutes the major mechanism to maintain a high GSH:GSSG ratio in the cytosol.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 18">
            <text>2 glutathione + NADP(+) = glutathione disulfide + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11740"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:58297"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.8.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="15">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 18">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 17">Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.8.1.7" evidence="18"/>
    <dbReference type="EMBL" id="EAAA01002428">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F7AVB6"/>
    <dbReference type="FunCoup" id="F7AVB6">
        <property type="interactions" value="246"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000007825"/>
    <dbReference type="Ensembl" id="ENSCINT00000007825.3">
        <property type="protein sequence ID" value="ENSCINP00000007825.3"/>
        <property type="gene ID" value="ENSCING00000003772.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156986"/>
    <dbReference type="HOGENOM" id="CLU_016755_2_2_1"/>
    <dbReference type="InParanoid" id="F7AVB6"/>
    <dbReference type="OMA" id="MSKHYDY"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004362">
        <property type="term" value="F:glutathione-disulfide reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.390.30:FF:000003">
        <property type="entry name" value="Glutathione reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000141">
        <property type="entry name" value="Glutathione reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000484">
        <property type="entry name" value="Glutathione reductase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006322">
        <property type="entry name" value="Glutathione_Rdtase_euk/bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046952">
        <property type="entry name" value="GSHR/TRXR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001100">
        <property type="entry name" value="Pyr_nuc-diS_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012999">
        <property type="entry name" value="Pyr_OxRdtase_I_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01421">
        <property type="entry name" value="gluta_reduc_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004776">
        <property type="entry name" value="PRK06116.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42737">
        <property type="entry name" value="GLUTATHIONE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42737:SF2">
        <property type="entry name" value="GLUTATHIONE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000350">
        <property type="entry name" value="Mercury_reductase_MerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="FAD/NAD-linked reductases, dimerisation (C-terminal) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00076">
        <property type="entry name" value="PYRIDINE_REDOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 18" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 15 17" id="KW-0274">FAD</keyword>
    <keyword evidence="5 17" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="15" id="KW-0520">NAD</keyword>
    <keyword evidence="7 18" id="KW-0521">NADP</keyword>
    <keyword evidence="15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 17" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12 17" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="20">
        <location>
            <begin position="13"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="Pyridine nucleotide-disulphide oxidoreductase dimerisation" evidence="19">
        <location>
            <begin position="361"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="14">
        <location>
            <position position="461"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="188"/>
            <end position="195"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="285"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="325"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="16">
        <location>
            <begin position="49"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU003691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU365016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF02852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000007825.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="472" mass="51051" checksum="C1E5E35314DF01D7" modified="2012-04-18" version="2">ITRRMASSVTKVDYLVIGGGSGGIASARRATEFGVSAVLIEHGPLGGTCVNVGCVPKKVMFNTAMHAEMIHDHKDYGFNLGGGVTFDWSVIKQKRDAYIKRLNGIYATNLDKAKVNTVHGHARFTGESDPHPIVEVGDKKFTAPHILIATGGYPVIPSNDEIPGASHGITSDGFFELEDLPKKTVVVGAGYIAVELAGILNALGSETRLVIRRHQALRAFDSMISENVTHEITSSGITLDRFSHVKKVVKDSTTGKMTITIDSTQDGTTTSTVVENVECLIWAIGRKPNTDMNLDKVGVKLDNRGNIVVDKFQNTNIEGVHAVGDVQGKALLTPVAIAAGRRLAHRLFDGKTDLFLDYTNIPTVVFSHPPIGTIGYTEAEAKKLHGDDKIKTYKAMFTPMYHAVTERKTKVAMKLVCLLPTEQVIGLHMQGLGCDEMLQGFGVAVKMGATKAQFDECIAIHPTSSEELVTMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>F7AYY6</accession>
    <name>F7AYY6_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Sushi domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="F7AYY6"/>
    <dbReference type="Ensembl" id="ENSCINT00000021551.3">
        <property type="protein sequence ID" value="ENSCINP00000021551.3"/>
        <property type="gene ID" value="ENSCING00000010970.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000235817"/>
    <dbReference type="HOGENOM" id="CLU_1222057_0_0_1"/>
    <dbReference type="InParanoid" id="F7AYY6"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003353602" description="Sushi domain-containing protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000021551.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="199" mass="21014" checksum="A4716263127E5D22" modified="2012-04-18" version="2">MSYQTIMFLMLVIFGSLCATAFSTCSNPTVTPSRITWALDYTGLLYSGYFCVTVQANDLTWSFEDSSVANIAVFTFPSGLGTNPCQPSTPVPGYNATCQYNTAGIPAGILGCYTTTFSVGNKLTSSFNQSTLKLAHGVSSCFAQNISLQACSSPTPNTVLVNPSSGVYHTQANFQCSHGSTLFNMDGTTGNTATKCLAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>F7B6N0</accession>
    <name>F7B6N0_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Plastocyanin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">wild type</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the multicopper oxidase family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002367">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F7B6N0"/>
    <dbReference type="STRING" id="7719.ENSCINP00000022666"/>
    <dbReference type="Ensembl" id="ENSCINT00000022912.2">
        <property type="protein sequence ID" value="ENSCINP00000022666.2"/>
        <property type="gene ID" value="ENSCING00000012025.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00440000034776"/>
    <dbReference type="HOGENOM" id="CLU_095180_0_0_1"/>
    <dbReference type="InParanoid" id="F7B6N0"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13853">
        <property type="entry name" value="CuRO_1_Tth-MCO_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF518">
        <property type="entry name" value="MULTICOPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003348192" description="Plastocyanin-like domain-containing protein" evidence="2">
        <location>
            <begin position="24"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="3">
        <location>
            <begin position="121"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000022666.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="207" mass="23604" checksum="050790CD729ACB46" modified="2011-07-27" version="1">MLPLSSLHQLVSVILFIASLTLGQQSLPCSNTNPCRRFRVDELYNEPTFYQSRARRLDITMSVEMAEIRFDWLKIWRRTYNGAIGGPTWMVYPGDTLNLRLENKLGPNPVNDTGPNTYHKPNTTNIHVHGLHISPEGISDNVFLTVEPGAVQQYQFNIPEHHPAGTYWYHPHFHGSGNFQVSGGMAGLIIIQDNPYHFDTPYELRMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>F7B946</accession>
    <name>F7B946_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01001371">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F7B946"/>
    <dbReference type="FunCoup" id="F7B946">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000022103"/>
    <dbReference type="Ensembl" id="ENSCINT00000022349.2">
        <property type="protein sequence ID" value="ENSCINP00000022103.2"/>
        <property type="gene ID" value="ENSCING00000007417.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158554"/>
    <dbReference type="HOGENOM" id="CLU_528520_0_0_1"/>
    <dbReference type="InParanoid" id="F7B946"/>
    <dbReference type="OMA" id="IEMANHK"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000002">
        <property type="entry name" value="TBC1 domain family member 9B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000049">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF6">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="1">
        <location>
            <begin position="10"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000022103.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="445" mass="51350" checksum="FDA94979A256710D" modified="2012-04-18" version="2">PKTKELIMKGIPERYRGEMWMVYSGAIIEMANHKGYYQSILKQCMGKCTLATDEIERDLHRSLPEHPAFQASEGIDALRRVLTAYAFRNPSIGYCQAMNIVTSVLLLYANEEESFWLLVSLCERLLPDYYNTRVVGALVDQGVFDELTKQHLPKIHDKLEVLGVVRTVTLSWFLTLFLCSMPFNSAVRVVDAFFYDGAQVVFQIALYVLKANEDVILKCNDDGEAMTILANYLNQVGNRDINTDQTKLLSHVDVSDLITKSYEHYSQVSSEVIENMRFRQRLEVVQRLEDSATRSFMRTMQELTKFPTDILQGLYFLFKAEFMKRSYWSRCLPKEDKTVDPSNFILEQYTIDYEQFRTIFGALSQWSKPPPDPLLDQIYRLCANKEGLINFQEFAVRLSTISIGDHHAKLRLLYDLHLHPALPTMNENAEVGVEAMHYFDDSDVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>F7BAB3</accession>
    <name>F7BAB3_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Zinc transporter</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">wild type</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ZIP transporter (TC 2.A.5) family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01000217">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F7BAB3"/>
    <dbReference type="STRING" id="7719.ENSCINP00000006884"/>
    <dbReference type="Ensembl" id="ENSCINT00000006884.3">
        <property type="protein sequence ID" value="ENSCINP00000006884.3"/>
        <property type="gene ID" value="ENSCING00000003358.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000169482"/>
    <dbReference type="HOGENOM" id="CLU_703105_0_0_1"/>
    <dbReference type="InParanoid" id="F7BAB3"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046873">
        <property type="term" value="F:metal ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003689">
        <property type="entry name" value="ZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050799">
        <property type="entry name" value="ZIP_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12191">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12191:SF30">
        <property type="entry name" value="ZINC TRANSPORTER ZIP4 N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02535">
        <property type="entry name" value="Zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="231"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="265"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="320"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000006884.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="393" mass="44593" checksum="B880A6DF5B496C74" modified="2012-04-18" version="2">MRFFLCSEQLGWHSNLNKKTYLTWNYRFVIGLVALTFALASHEDSETKQTTLSNLTQTSKPNLIITSCTADGCIFQVNTTQVLADEFIKYTEIVFKRYSMPGFSYINIDGLNHLLIDLGVEETTSDDHDHDHDDHDDHDEYAIPEYYSYDENNIDYDNIEKPKCRKAIDILSEYYPYVNAYDVRIGLDDFITILPNVVDSVYECRNLSLSKQGADKHEHSSQHSTENRGKVWGIAILSVTVISVLSVIGILIVPLMRHNDHFNRLISFLVALAVGTLTGDALLHLLPHAMGEHNHTSADHAHDHGNEDKNKEKEKLMKSLVAVVGMYMFFVMESLMGIARQRKNMKKMKNEIPNPIKVSPTDSSVGSPIKSDVSFSTRMECTDSLLIPETDLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>F7BKY1</accession>
    <name>F7BKY1_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sema domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">wild type</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002017">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F7BKY1"/>
    <dbReference type="STRING" id="7719.ENSCINP00000024714"/>
    <dbReference type="Ensembl" id="ENSCINT00000024960.2">
        <property type="protein sequence ID" value="ENSCINP00000024714.2"/>
        <property type="gene ID" value="ENSCING00000013472.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159170"/>
    <dbReference type="HOGENOM" id="CLU_1212154_0_0_1"/>
    <dbReference type="InParanoid" id="F7BKY1"/>
    <dbReference type="OMA" id="CATESFQ"/>
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        <property type="component" value="Chromosome 4"/>
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    <dbReference type="GO" id="GO:0030215">
        <property type="term" value="F:semaphorin receptor binding"/>
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        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
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    <feature type="chain" id="PRO_5003348531" description="Sema domain-containing protein" evidence="2">
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        <location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000024714.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="229" mass="25926" checksum="263E4741359C80DA" modified="2012-04-18" version="2">MVYKVVTLKSMLVWLQFYMNVIASIPENISPTSVYSRKNTSIYPAFIGEGTNTNVSLQHVQGVTKIGDFFFIFAKDHIFTIDMREVNQTQFHFSHVFDWASTKDSKNLCLKKGMPSEKCHNYIKMIAMVQTINGNSKKLVTCATESFQPMCKYLKMDEEGNLTDLGESFRGTLTCPYDPTRSYASLLLPDGHWYTATEAAFGGHDAVLHRSSLMNGNEVRLRTEQSQSS</sequence>
</entry>
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            <fullName evidence="10">Transmembrane protein 9</fullName>
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        <name evidence="8" type="primary">LOC100183612</name>
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        <name type="scientific">Ciona intestinalis</name>
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            <taxon>Eukaryota</taxon>
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    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">wild type</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM9 family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002149">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
    <dbReference type="Ensembl" id="ENSCINT00000023167.2">
        <property type="protein sequence ID" value="ENSCINP00000022921.2"/>
        <property type="gene ID" value="ENSCING00000012217.2"/>
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    <dbReference type="GeneID" id="100183612"/>
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    <dbReference type="InParanoid" id="F7BP46"/>
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        <property type="component" value="Chromosome 5"/>
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        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="entry name" value="TMEM9/TMEM9B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13064:SF6">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13064">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 9 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05434">
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
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    <feature type="transmembrane region" description="Helical" evidence="6">
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    <evidence type="ECO:0000256" key="1">
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    <evidence type="ECO:0000256" key="2">
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    <evidence type="ECO:0000256" key="3">
        <source>
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    </evidence>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="203" mass="22891" checksum="0C6AA0F5D1EB5799" modified="2012-04-18" version="2">MYCGILLFVLLSFQLLENTSAQYEDIRCKCTCLAYHKYNIPAKIYTKNVLHEYCDCLRVVLPNLNGSALTDVDIEPYCLRCGCKYEVRSTKTMQVVVYMFLVLLGVLLIYMIVMVIADPIINHVNRKNRAAGLLDEETEMESIVSARDSDRPRSSSVGTGLWAISACLTKGKSNANKMEKAASRAKIKCVRPTRDVTMRSMKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
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    <name>H2XKH5_CIOIN</name>
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            <fullName evidence="1">N-acetyltransferase domain-containing protein</fullName>
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        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
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                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">wild type</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01001639">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XN05"/>
    <dbReference type="Ensembl" id="ENSCINT00000035029.1">
        <property type="protein sequence ID" value="ENSCINP00000031038.1"/>
        <property type="gene ID" value="ENSCING00000024637.1"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2660808_0_0_1"/>
    <dbReference type="InParanoid" id="H2XN05"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000031038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="76" mass="8705" checksum="A66E236C58437FA3" modified="2012-04-18" version="1">CWVPGFLQKLPSRPASGKRWELVLVRDHGVIPLLRNCCVVQQARVELPNCHSSHLLEPHFQSTASLFPCDLPWNIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>H2XND6</accession>
    <accession>A0A1W2W5D0</accession>
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        <submittedName>
            <fullName evidence="3">Uncharacterized LOC100183766</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100183766</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01000405">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002119228.1">
        <property type="nucleotide sequence ID" value="XM_002119192.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XND6"/>
    <dbReference type="Ensembl" id="ENSCINT00000033099.1">
        <property type="protein sequence ID" value="ENSCINP00000031169.1"/>
        <property type="gene ID" value="ENSCING00000018808.1"/>
    </dbReference>
    <dbReference type="GeneID" id="100183766"/>
    <dbReference type="KEGG" id="cin:100183766"/>
    <dbReference type="GeneTree" id="ENSGT00660000097103"/>
    <dbReference type="HOGENOM" id="CLU_1204427_0_0_1"/>
    <dbReference type="InParanoid" id="H2XND6"/>
    <dbReference type="OMA" id="VLRNAYD"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00037">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
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        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
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        <location>
            <begin position="21"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="2">
        <location>
            <begin position="85"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000031169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="230" mass="26793" checksum="610B69D03CCDD80A" modified="2012-04-18" version="1">MKSLAFAFVFLTMCWSFVSCENNDVCSNVTESYKICTAVRTLIRHQNACDPNHVRVLERRIQVLTGTLNRLTIQKRRGECSGVIYRGKCYFLLDDGCEPNMRSYQESKEACEAQQGHLVKIRDAANYNFLFNYVLRNAYDFMSENRDFVLVWTGGAWDYERGRVRFENNQTTTYSPWNVGHPLNMTDRTNIAMAVATRPILRGMIDVPPSNQFYPLCEKVMRCRYGKRNR</sequence>
</entry>
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    <accession>H2XNH1</accession>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
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                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
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                <person name="Rigoutsos I."/>
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                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
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                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
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                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <dbReference type="STRING" id="7719.ENSCINP00000031204"/>
    <dbReference type="Ensembl" id="ENSCINT00000034045.1">
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        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="entry name" value="Hobbit"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
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            <end position="191"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="159"/>
            <end position="172"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="182"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000031204.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
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    </evidence>
    <sequence length="191" mass="21776" checksum="B604C095687F43B7" modified="2012-04-18" version="1">MLLYSTTLRWLSNFGYAVNAVSRPVCKGPIFGNQAPPKLKLSRHYRNVKPSFQFPELKVSYWSSYAQQLGIEWICGAGKIDSHFRLNLVPYEGNLIRRSMGQWQIMATTCELSNTKIYLLKRQESSSQNQSPSNRSLGAERNFLLSLSSMSYRRDNVSDGLNTPDNLSPTKHSTFKGISDPQARKKEFEET</sequence>
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<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>H2XNP6</accession>
    <name>H2XNP6_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Intraflagellar transport protein 27 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100177670</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="H2XNP6"/>
    <dbReference type="FunCoup" id="H2XNP6">
        <property type="interactions" value="126"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000031279"/>
    <dbReference type="Ensembl" id="ENSCINT00000035646.1">
        <property type="protein sequence ID" value="ENSCINP00000031279.1"/>
        <property type="gene ID" value="ENSCING00000020075.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00870000136549"/>
    <dbReference type="HOGENOM" id="CLU_041217_10_6_1"/>
    <dbReference type="InParanoid" id="H2XNP6"/>
    <dbReference type="OMA" id="KMWGQPS"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030992">
        <property type="term" value="C:intraciliary transport particle B"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042073">
        <property type="term" value="P:intraciliary transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47978">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000031279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="152" mass="17294" checksum="4EA8303FD56246AB" modified="2012-04-18" version="1">MMSTGVDLLQKFVQLGEDNKDVVDLFMFDSAGKEVFHQLNEASWDKVGMFVLVYDITNKTSFKNCQQWLKKIKQFSEPGKSIPGVVIGNKGDLKDRREVEANEGNAFAVKHGLVFFECSAKQYEGIDVVFKHLANEVHQLYQQSSENVQQIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>H2XPR2</accession>
    <name>H2XPR2_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="H2XPR2"/>
    <dbReference type="Ensembl" id="ENSCINT00000034375.1">
        <property type="protein sequence ID" value="ENSCINP00000031646.1"/>
        <property type="gene ID" value="ENSCING00000021670.1"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2209083_0_0_1"/>
    <dbReference type="InParanoid" id="H2XPR2"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000031646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="107" mass="12038" checksum="022D20CA98BCC95D" modified="2012-04-18" version="1">MSHGSKAGVYTLLGPRIRIAVQWNFSLNRNNGVEIFSIGAPNRHSTIAPPSCSVVLFLLGLADFRNQRLFRHFLLGLATAHAIEQEHQHKKANDWYRHVGPTIVIQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>H2XPS6</accession>
    <name>H2XPS6_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">wild type</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
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                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the beta type-B retroviral polymerase family. HERV class-II K(HML-2) pol subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.4" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="H2XQV4"/>
    <dbReference type="STRING" id="7719.ENSCINP00000032038"/>
    <dbReference type="Ensembl" id="ENSCINT00000035281.1">
        <property type="protein sequence ID" value="ENSCINP00000032038.1"/>
        <property type="gene ID" value="ENSCING00000021231.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01100000263500"/>
    <dbReference type="HOGENOM" id="CLU_000384_9_3_1"/>
    <dbReference type="InParanoid" id="H2XQV4"/>
    <dbReference type="OMA" id="RNINDAG"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09274">
        <property type="entry name" value="RNase_HI_RT_Ty3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.270:FF:000023">
        <property type="entry name" value="Pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000331">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041577">
        <property type="entry name" value="RT_RNaseH_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17919">
        <property type="entry name" value="RT_RNaseH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="5">
        <location>
            <begin position="542"/>
            <end position="717"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00039658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000032038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="812" mass="91924" checksum="D3DAAC9E27E615A2" modified="2012-04-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>H2XQX8</accession>
    <accession>A0A1W2WR38</accession>
    <name>H2XQX8_CIOIN</name>
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        <submittedName>
            <fullName evidence="15">Potassium channel subfamily K member 10</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100183963</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">wild type</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the two pore domain potassium channel (TC 1.A.1.8) family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002265">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_004226141.1">
        <property type="nucleotide sequence ID" value="XM_004226093.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XQX8"/>
    <dbReference type="STRING" id="7719.ENSCINP00000032062"/>
    <dbReference type="Ensembl" id="ENSCINT00000035674.1">
        <property type="protein sequence ID" value="ENSCINP00000032062.1"/>
        <property type="gene ID" value="ENSCING00000022352.1"/>
    </dbReference>
    <dbReference type="GeneID" id="100183963"/>
    <dbReference type="KEGG" id="cin:100183963"/>
    <dbReference type="GeneTree" id="ENSGT00940000168115"/>
    <dbReference type="HOGENOM" id="CLU_022504_1_1_1"/>
    <dbReference type="InParanoid" id="H2XQX8"/>
    <dbReference type="OMA" id="CQRTITE"/>
    <dbReference type="OrthoDB" id="297496at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015271">
        <property type="term" value="F:outward rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022841">
        <property type="term" value="F:potassium ion leak channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071805">
        <property type="term" value="P:potassium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000193">
        <property type="entry name" value="Uncharacterized protein, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003280">
        <property type="entry name" value="2pore_dom_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003092">
        <property type="entry name" value="2pore_dom_K_chnl_TASK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11003:SF330">
        <property type="entry name" value="POTASSIUM CHANNEL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11003">
        <property type="entry name" value="POTASSIUM CHANNEL, SUBFAMILY K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01333">
        <property type="entry name" value="2POREKCHANEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01095">
        <property type="entry name" value="TASKCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9 12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0630">Potassium</keyword>
    <keyword evidence="6" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="100"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="125"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="175"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="208"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="240"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="14">
        <location>
            <begin position="95"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="14">
        <location>
            <begin position="185"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
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            <dbReference type="RuleBase" id="RU003857"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF07885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000032062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="324" mass="35998" checksum="889A33C2F2D7FED4" modified="2012-04-18" version="1">MKWLYILVLIFLYFVYLLVGAGVFVALEFSREELECQRTITEIGEVAAKVNSNLSNANGSNDNLTGEDFRNLIQLIREAPQNGVQFDSNQTASCAHNWDFHNALFFAGTVVTTIGYGNISPQTFGGRVFCIFYAAIGIPLFAIMFAAIGEKLSKLFKRLDTKLTKKTRSSILRKAFVFVVTAGTLLLFCCVIPAFVFVAVEKWDYNEAFYYSFITLTTIGFGDFVVGTDADLSYATIYKVAVYLWILFGLAYMATVISIVSGYMHDTAKPKKNDSNTSAKPTEDGVDGEFHVTEDEVIPNKYIHENELPLDGDSTSNKVSTVAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>H2XRA3</accession>
    <name>H2XRA3_CIOIN</name>
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        <recommendedName>
            <fullName evidence="4">p53 and DNA damage-regulated protein 1</fullName>
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    <organism evidence="9 10">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Ciona</taxon>
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    <reference evidence="10" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">wild type</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="1">May play a role in chaperone-mediated protein folding.</text>
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    <comment type="subunit">
        <text evidence="7">Component of the PAQosome complex which is responsible for the biogenesis of several protein complexes and which consists of R2TP complex members RUVBL1, RUVBL2, RPAP3 and PIH1D1, URI complex members PFDN2, PFDN6, PDRG1, UXT and URI1 as well as ASDURF, POLR2E and DNAAF10/WDR92.</text>
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        <subcellularLocation>
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        <text evidence="3">Belongs to the prefoldin subunit beta family.</text>
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        <property type="gene ID" value="ENSCING00000020722.1"/>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="Prefoldin"/>
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    </dbReference>
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        <location>
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        <source>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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</entry>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Chapman J."/>
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                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
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                <person name="Kobayashi K."/>
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                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
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                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
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                <person name="Nishizaka S."/>
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                <person name="Oishi K."/>
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                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
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                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
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                <person name="Levine M."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
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            <dbReference type="PubMed" id="18854010"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">wild type</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="ENSCING00000021973.1"/>
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    <dbReference type="HOGENOM" id="CLU_3013459_0_0_1"/>
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        <property type="component" value="Chromosome 12"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000033156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="56" mass="6528" checksum="8AB41C1AD58EBC2A" modified="2012-04-18" version="1">MRIAFTTNTTLHRATIALEDSSPREHRFSNNSCIFQLLHTMQLQELISYKPISTTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>H2XU33</accession>
    <name>H2XU33_CIOIN</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
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    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
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                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">wild type</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Adherens junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the vezatin family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002133">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XWM9"/>
    <dbReference type="FunCoup" id="H2XWM9">
        <property type="interactions" value="16"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000034063"/>
    <dbReference type="Ensembl" id="ENSCINT00000033717.1">
        <property type="protein sequence ID" value="ENSCINP00000034063.1"/>
        <property type="gene ID" value="ENSCING00000022104.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000003290"/>
    <dbReference type="HOGENOM" id="CLU_546221_0_0_1"/>
    <dbReference type="InParanoid" id="H2XWM9"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017022">
        <property type="term" value="F:myosin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026859">
        <property type="entry name" value="Myosin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026858">
        <property type="entry name" value="Vezatin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15989">
        <property type="entry name" value="VEZATIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15989:SF5">
        <property type="entry name" value="VEZATIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12632">
        <property type="entry name" value="Vezatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0965">Cell junction</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Myosin-binding" evidence="14">
        <location>
            <begin position="5"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="407"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="415"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF12632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000034063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="499" mass="55913" checksum="3E5A0A5E205E85A9" modified="2012-04-18" version="1">MNRGSARMFMKLRYNVMRSIVKCTLVCRTATNQLLEYSLQDSCDLEEYFICKVPLSKLGVCFMENSTPSEMFNATDGYSLSALKCLNELLCKQRSEMLTRLSLTLSGTTWDFTSHGFLSRFTSILDQPNTVTADSLQSLQCSYDMHKYVTNTKNIPQQSQGEGQPCKTSVLRINLHSLQVHMRAAMHAAMQLEESLPLDNTQVQLPDNFINIGRHLESGLDCWAQFNKEISGFEENESEKEETENIVQPVVTEDEELVVVDNTDDEVTMEDQIFEAIADDSSDEDYDRGDMFDSRPTKIVEDTATVMRELRSVLAVKTSEKEREMWKMKLFPGYKGKKVEDQLKNEELSIEEKSNNPEENCEVDIVDTSDQSVSCDLNNINNSKLKGDDLEVDPQASDTLNGEIRSQMLSFPKRSAPKGRRPPTNKRAHNTRNGVVTMPTIESQSTSTDSDDNQTDPDWTSGLGIGFPGAIALQAAAMARSKQIGCGQITTYGSDSDSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>H2XX80</accession>
    <name>H2XX80_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01002133">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XX80"/>
    <dbReference type="STRING" id="7719.ENSCINP00000034264"/>
    <dbReference type="Ensembl" id="ENSCINT00000033425.1">
        <property type="protein sequence ID" value="ENSCINP00000034264.1"/>
        <property type="gene ID" value="ENSCING00000023475.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000012843"/>
    <dbReference type="HOGENOM" id="CLU_964829_0_0_1"/>
    <dbReference type="InParanoid" id="H2XX80"/>
    <dbReference type="OMA" id="NEFITSP"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032502">
        <property type="term" value="P:developmental process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050283">
        <property type="entry name" value="E-box_TF_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23349">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR, TWIST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23349:SF72">
        <property type="entry name" value="HLH54F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BHLH" evidence="1">
        <location>
            <begin position="35"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000034264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="293" mass="33886" checksum="D382FAE9C29265E6" modified="2012-04-18" version="1">MTSLQYEQVSRTFSTNKVNSRKRRSDDVTIGVAKKRRSNSAGKERDRLHVFNQALETLQSVIPVRLTEQRNLHKKQTLQLAIRYINFLRGCLDGTRDWKERNRFWCRTQDEMVIDAIDQAAAGHNVHCDVTSPQMTSPESESPPPPSGCYTQINYETVQPSIMNHNQDCSPQLRHWNEESQYCDVTYSLETTSIQNPQNYDQLYSQYDPTTSFLTSYFQNNSIRQDDVTYLNKESDELFTSLTDDVSTSIDDITPTFPYSNMPMNNVFNEFITSPENLLRHPDSPDSGYYSLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>H2XY50</accession>
    <name>H2XY50_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NXPE family member 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC100181170</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
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<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>H2XZP2</accession>
    <name>H2XZP2_CIOIN</name>
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        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">wild type</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GPAT/DAPAT family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002619">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XZP2"/>
    <dbReference type="STRING" id="7719.ENSCINP00000035126"/>
    <dbReference type="Ensembl" id="ENSCINT00000031941.1">
        <property type="protein sequence ID" value="ENSCINP00000035126.1"/>
        <property type="gene ID" value="ENSCING00000024592.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00520000055570"/>
    <dbReference type="HOGENOM" id="CLU_1464413_0_0_1"/>
    <dbReference type="InParanoid" id="H2XZP2"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008374">
        <property type="term" value="F:O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07993">
        <property type="entry name" value="LPLAT_DHAPAT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022284">
        <property type="entry name" value="GPAT/DHAPAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045520">
        <property type="entry name" value="GPAT/DHAPAT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041728">
        <property type="entry name" value="GPAT/DHAPAT_LPLAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12563:SF23">
        <property type="entry name" value="BCDNA.GH07066"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12563">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19277">
        <property type="entry name" value="GPAT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phospholipid/glycerol acyltransferase" evidence="6">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="GPAT/DHAPAT C-terminal" evidence="7">
        <location>
            <begin position="97"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF19277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000035126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="185" mass="20633" checksum="CE691C9A0B3840AA" modified="2012-04-18" version="1">MKRVGGFFIRRKIDSAGEKDLVYRAVLYSYVTELLKEKQSLEIFIEGTRTRSGLPNQPKTGLLAVAVEAVEKSQVDDIIIIPTCMSYEKLMEGNFDAELMGQTKIPETFLHSILTIGKMLCGFYGHIRVNFGAPQSLKKTLAEIRSSPACLSSKSQQSLPRSRSLYEQVTNKSMENKLSSSTPTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>H2Y180</accession>
    <name>H2Y180_CIOIN</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
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    <organism evidence="1 2">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
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        <lineage>
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            <taxon>Tunicata</taxon>
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            <taxon>Ciona</taxon>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">wild type</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01002969">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2Y180"/>
    <dbReference type="Ensembl" id="ENSCINT00000036460.1">
        <property type="protein sequence ID" value="ENSCINP00000035664.1"/>
        <property type="gene ID" value="ENSCING00000024064.1"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2811609_0_0_1"/>
    <dbReference type="InParanoid" id="H2Y180"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000035664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="67" mass="7290" checksum="3D6F917791074B0D" modified="2012-04-18" version="1">MSSTILGFNTTNTTTETSQNIDMTGHFTTTIVTHPSTTSNASYGPTRTTSVIAVNVLLTYVFYFTTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>H2Y1S9</accession>
    <name>H2Y1S9_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
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                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="H2Y1S9"/>
    <dbReference type="STRING" id="7719.ENSCINP00000035863"/>
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        <property type="protein sequence ID" value="ENSCINP00000035863.1"/>
        <property type="gene ID" value="ENSCING00000020158.1"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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    <dbReference type="InterPro" id="IPR051832">
        <property type="entry name" value="mTOR-Rac_regulators"/>
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    <dbReference type="PANTHER" id="PTHR22829">
        <property type="entry name" value="DEP DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22829:SF16">
        <property type="entry name" value="PH DOMAIN-CONTAINING PROTEIN"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000035863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="160" mass="17614" checksum="9250C20829D521F1" modified="2012-04-18" version="1">MALSVCEGTDQFADVINTLIESVDRHVNEVRTQLVLAVLLNEDPKLVSRRDKVFSQALVASISAFSAQLRCAIAGTFNNMKTYGVRPEEASKKWLEQSMSIGAAVIFQSMLNPNMPDENAMLEDSCIGINDLERVSFHFCAITGEVEPNGESASIFMTQH</sequence>
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<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>H2Y2N2</accession>
    <name>H2Y2N2_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">MPN domain-containing protein</fullName>
        </recommendedName>
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    <organism evidence="11 12">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
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            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">wild type</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M67C family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002293">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2Y2N2"/>
    <dbReference type="FunCoup" id="H2Y2N2">
        <property type="interactions" value="363"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000036167"/>
    <dbReference type="MEROPS" id="M67.A09"/>
    <dbReference type="Ensembl" id="ENSCINT00000032532.1">
        <property type="protein sequence ID" value="ENSCINP00000036167.1"/>
        <property type="gene ID" value="ENSCING00000022439.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153710"/>
    <dbReference type="HOGENOM" id="CLU_023304_4_0_1"/>
    <dbReference type="InParanoid" id="H2Y2N2"/>
    <dbReference type="OMA" id="MKFMTLF"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061578">
        <property type="term" value="F:K63-linked deubiquitinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140492">
        <property type="term" value="F:metal-dependent deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:protein K63-linked deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08066">
        <property type="entry name" value="MPN_AMSH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.10:FF:000033">
        <property type="entry name" value="AMSH-like protease sst2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.80">
        <property type="entry name" value="Phosphotransferase system, lactose/cellobiose-type IIA subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000555">
        <property type="entry name" value="JAMM/MPN+_dom"/>
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    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
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        <property type="entry name" value="STAMBP/STALP-like_MPN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015063">
        <property type="entry name" value="USP8_dimer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12947">
        <property type="entry name" value="AMSH-LIKE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12947:SF13">
        <property type="entry name" value="FI19924P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01398">
        <property type="entry name" value="JAB"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="USP8_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00232">
        <property type="entry name" value="JAB_MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102712">
        <property type="entry name" value="JAB1/MPN domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140856">
        <property type="entry name" value="USP8 N-terminal domain-like"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
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    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="MPN" evidence="10">
        <location>
            <begin position="249"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="116"/>
            <end position="135"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000036167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="423" mass="48910" checksum="BA142D70E888BF96" modified="2012-04-18" version="1">MASSHIEPTVRLRNLFDKGSAVEVNRNIPIRRYYRSCQELIRMAGVYFEEKNYENSYVYYAKFIVLSFEKLKTHPEFNKVPPAEKAANKKTLKIAFDRAEKLKPALKRKYEEEYKRYKENQKQEEEKRKKEEEEERKRKEEIKKKAEEEQMNAFIQEQRRKELELERLAIVAEFADKVKVDDNGPLVPADFAPPPPEPFNDIATNTTTYKPQPTVPTPPALPSYDRSLKPDKHIFSSLTQSKNKYGLRTILSPADLPTKFMQLAHSNTSRNIETCGVLFGKLANEVFVITHVLIPHQKGAPDSCDTTREEDMWDFQDQYDGICLGWIHTHPSQTAFLSSVDMHTHYPYQCLMPESVAIVCSGKFNEVGYFMLDPGRGMNEIGKCRKPGFHPHPTTPPLFESCDHVKPSPTDAVHIVDWRLKPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>H2Y361</accession>
    <name>H2Y361_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
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            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
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                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">wild type</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01002744">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2Y3P1"/>
    <dbReference type="Ensembl" id="ENSCINT00000036912.1">
        <property type="protein sequence ID" value="ENSCINP00000036526.1"/>
        <property type="gene ID" value="ENSCING00000023148.1"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2848999_0_0_1"/>
    <dbReference type="InParanoid" id="H2Y3P1"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000036526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="65" mass="7688" checksum="89AAA4AB0B13C902" modified="2012-04-18" version="1">MKFSLSFKLNVQLICFLMVPFTVYKFRKSFMGKQCMVELKSCEFLLLCGNGKFRCQFISLFIRVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-03-06" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>L7N0R5</accession>
    <name>L7N0R5_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Enoyl-CoA hydratase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001500">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="L7N0R5"/>
    <dbReference type="STRING" id="7719.ENSCINP00000007451"/>
    <dbReference type="Ensembl" id="ENSCINT00000007451.3">
        <property type="protein sequence ID" value="ENSCINP00000007451.3"/>
        <property type="gene ID" value="ENSCING00000003611.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000167986"/>
    <dbReference type="HOGENOM" id="CLU_009834_7_4_1"/>
    <dbReference type="InParanoid" id="L7N0R5"/>
    <dbReference type="OMA" id="PKTDSWH"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018376">
        <property type="entry name" value="Enoyl-CoA_hyd/isom_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006108">
        <property type="entry name" value="PRK08259.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43802">
        <property type="entry name" value="ENOYL-COA HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43802:SF1">
        <property type="entry name" value="IP11341P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00166">
        <property type="entry name" value="ENOYL_COA_HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000007451.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="299" mass="32523" checksum="050150AAB3913955" modified="2013-03-06" version="1">MYRQCLNKLHQTVKLQPIGNALCVCNRKLTLDPQSEPAVDPITIDKRDKVTLIGLNRPGSRNAVNRDMARQLTVAFLAFEQDNSSNVAVLHGKGDHFCAGYDLKELAHSESGIDEETMNHLHNVHGAMGPTRLHIKKPVIAAVNGYGVAGGLELALLCDLRVFEESTIVGVFNRRFGVPLVDGGTVRLPALVGLSNALDLILTGRAIDSEEAIRIGLANRVVKDGEALSFAVELAKEISHHPQMSLRADRDSAYNAMFNSTSKKESLDYEMNHALDVVAHESIAGAKRFKDGEGRQGVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-16" modified="2026-01-28" version="121" xmlns="https://uniprot.org/uniprot">
    <accession>Q1RL25</accession>
    <accession>A0A1W2VNJ4</accession>
    <accession>F7A7Y1</accession>
    <name>Q1RL25_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ubiquitin carboxyl-terminal hydrolase</fullName>
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        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">Ci-ZF(UBP)-3</name>
        <name evidence="12" type="synonym">zf(ubp)-3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2006" name="Dev. Biol." volume="292" first="546" last="554">
            <title>Systematic analysis of embryonic expression profiles of zinc finger genes in Ciona intestinalis.</title>
            <authorList>
                <person name="Miwata K."/>
                <person name="Chiba T."/>
                <person name="Horii R."/>
                <person name="Yamada L."/>
                <person name="Kubo A."/>
                <person name="Miyamura D."/>
                <person name="Satoh N."/>
                <person name="Satou Y."/>
            </authorList>
            <dbReference type="PubMed" id="16519883"/>
            <dbReference type="DOI" id="10.1016/j.ydbio.2006.01.024"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">wild type</strain>
        </source>
    </reference>
    <reference evidence="12" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="7"/>
    <dbReference type="EMBL" id="EAAA01002615">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="BR000209">
        <property type="protein sequence ID" value="FAA00240.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001041464.1">
        <property type="nucleotide sequence ID" value="NM_001047999.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1RL25"/>
    <dbReference type="STRING" id="7719.ENSCINP00000013776"/>
    <dbReference type="Ensembl" id="ENSCINT00000013776.3">
        <property type="protein sequence ID" value="ENSCINP00000013776.3"/>
        <property type="gene ID" value="ENSCING00000006716.3"/>
    </dbReference>
    <dbReference type="GeneID" id="751572"/>
    <dbReference type="KEGG" id="cin:751572"/>
    <dbReference type="CTD" id="751572"/>
    <dbReference type="GeneTree" id="ENSGT00940000160526"/>
    <dbReference type="HOGENOM" id="CLU_008279_13_1_1"/>
    <dbReference type="OMA" id="ERYHCLQ"/>
    <dbReference type="OrthoDB" id="21192at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="P:protein deubiquitination"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
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        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
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        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
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        <property type="entry name" value="USP"/>
    </dbReference>
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        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001607">
        <property type="entry name" value="Znf_UBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF5">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="zf-UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00290">
        <property type="entry name" value="ZnF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50271">
        <property type="entry name" value="ZF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="4 7" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBP-type" evidence="10">
        <location>
            <begin position="53"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="9">
        <location>
            <begin position="539"/>
            <end position="960"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="325"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="435"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="461"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="13"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="332"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="358"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="389"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="470"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00502"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="FAA00240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000013776.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="993" mass="113531" checksum="76F8EE392D9325F5" modified="2006-05-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-16" modified="2026-01-28" version="114" xmlns="https://uniprot.org/uniprot">
    <accession>Q1RL58</accession>
    <accession>F6UEQ8</accession>
    <name>Q1RL58_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">DNA ligase</fullName>
            <ecNumber evidence="19">6.5.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="primary">Ci-ZF(PARP)-2</name>
        <name evidence="26" type="synonym">zf(parp)-2</name>
    </gene>
    <organism evidence="25">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="journal article" date="2006" name="Dev. Biol." volume="292" first="546" last="554">
            <title>Systematic analysis of embryonic expression profiles of zinc finger genes in Ciona intestinalis.</title>
            <authorList>
                <person name="Miwata K."/>
                <person name="Chiba T."/>
                <person name="Horii R."/>
                <person name="Yamada L."/>
                <person name="Kubo A."/>
                <person name="Miyamura D."/>
                <person name="Satoh N."/>
                <person name="Satou Y."/>
            </authorList>
            <dbReference type="PubMed" id="16519883"/>
            <dbReference type="DOI" id="10.1016/j.ydbio.2006.01.024"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18 19">
            <text>ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + diphosphate.</text>
            <dbReference type="EC" id="6.5.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 20">Belongs to the ATP-dependent DNA ligase family.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.1" evidence="19"/>
    <dbReference type="EMBL" id="BR000176">
        <property type="protein sequence ID" value="FAA00207.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1RL58"/>
    <dbReference type="STRING" id="7719.ENSCINP00000005256"/>
    <dbReference type="Ensembl" id="ENSCINT00000005256.3">
        <property type="protein sequence ID" value="ENSCINP00000005256.3"/>
        <property type="gene ID" value="ENSCING00000002580.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156492"/>
    <dbReference type="HOGENOM" id="CLU_011787_0_0_1"/>
    <dbReference type="OMA" id="GRWCTVT"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003910">
        <property type="term" value="F:DNA ligase (ATP) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071897">
        <property type="term" value="P:DNA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097681">
        <property type="term" value="P:double-strand break repair via alternative nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006273">
        <property type="term" value="P:lagging strand elongation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07902">
        <property type="entry name" value="Adenylation_DNA_ligase_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07967">
        <property type="entry name" value="OBF_DNA_ligase_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3260.10:FF:000015">
        <property type="entry name" value="DNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000085">
        <property type="entry name" value="DNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1740.10:FF:000011">
        <property type="entry name" value="DNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.30:FF:000003">
        <property type="entry name" value="DNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3260.10">
        <property type="entry name" value="DNA ligase, ATP-dependent, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.30">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1740.10">
        <property type="entry name" value="Zinc finger, PARP-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050191">
        <property type="entry name" value="ATP-dep_DNA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000977">
        <property type="entry name" value="DNA_ligase_ATP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012309">
        <property type="entry name" value="DNA_ligase_ATP-dep_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012310">
        <property type="entry name" value="DNA_ligase_ATP-dep_cent"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016059">
        <property type="entry name" value="DNA_ligase_ATP-dep_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012308">
        <property type="entry name" value="DNA_ligase_ATP-dep_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036599">
        <property type="entry name" value="DNA_ligase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031916">
        <property type="entry name" value="LIG3_BRCT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001510">
        <property type="entry name" value="Znf_PARP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036957">
        <property type="entry name" value="Znf_PARP_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00574">
        <property type="entry name" value="dnl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45674">
        <property type="entry name" value="DNA LIGASE 1/3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45674:SF9">
        <property type="entry name" value="DNA LIGASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04679">
        <property type="entry name" value="DNA_ligase_A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01068">
        <property type="entry name" value="DNA_ligase_A_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04675">
        <property type="entry name" value="DNA_ligase_A_N"/>
        <property type="match status" value="1"/>
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        <name type="scientific">Ciona intestinalis</name>
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            <taxon>Phlebobranchia</taxon>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="7" key="2">
        <citation type="journal article" date="2003" name="Dev. Genes Evol." volume="213" first="211" last="212">
            <title>Genomewide surveys of developmentally relevant genes in Ciona intestinalis.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Satoh N."/>
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            <dbReference type="DOI" id="10.1007/s00427-003-0330-z"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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    <reference evidence="7" key="3">
        <citation type="journal article" date="2004" name="Development" volume="131" first="4047" last="4058">
            <title>Gene expression profiles of transcription factors and signaling molecules in the ascidian embryo: towards a comprehensive understanding of gene networks.</title>
            <authorList>
                <person name="Imai K.S."/>
                <person name="Hino K."/>
                <person name="Yagi K."/>
                <person name="Satoh N."/>
                <person name="Satou Y."/>
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            <dbReference type="PubMed" id="15269171"/>
            <dbReference type="DOI" id="10.1242/dev.01270"/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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    <reference evidence="7" key="4">
        <citation type="submission" date="2005-04" db="EMBL/GenBank/DDBJ databases">
            <title>Expressed genes in Ciona intestinalis.</title>
            <authorList>
                <person name="Satou Y."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="8" key="5">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="8">wild type</strain>
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        <citation type="submission" date="2025-05" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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</entry>
<entry dataset="TrEMBL" created="2004-07-05" modified="2026-01-28" version="126" xmlns="https://uniprot.org/uniprot">
    <accession>Q6XLI4</accession>
    <accession>A0A1W2VNB8</accession>
    <accession>F6ZD90</accession>
    <name>Q6XLI4_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="6 7">Swan</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rbm12</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2003-01" db="EMBL/GenBank/DDBJ databases">
            <title>Identification of a putative RNA binding protein in sea squirt.</title>
            <authorList>
                <person name="Chen Y."/>
                <person name="Huang C.-H."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AY226102">
        <property type="protein sequence ID" value="AAP48572.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001027771.1">
        <property type="nucleotide sequence ID" value="NM_001032599.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q6XLI4"/>
    <dbReference type="STRING" id="7719.ENSCINP00000002125"/>
    <dbReference type="Ensembl" id="ENSCINT00000002125.3">
        <property type="protein sequence ID" value="ENSCINP00000002125.3"/>
        <property type="gene ID" value="ENSCING00000001131.3"/>
    </dbReference>
    <dbReference type="GeneID" id="445800"/>
    <dbReference type="KEGG" id="cin:445800"/>
    <dbReference type="CTD" id="10137"/>
    <dbReference type="GeneTree" id="ENSGT00940000167419"/>
    <dbReference type="HOGENOM" id="CLU_294221_0_0_1"/>
    <dbReference type="OMA" id="MFQFANT"/>
    <dbReference type="OrthoDB" id="2588702at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043484">
        <property type="term" value="P:regulation of RNA splicing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd12510">
        <property type="entry name" value="RRM1_RBM12_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12515">
        <property type="entry name" value="RRM5_RBM12_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12254">
        <property type="entry name" value="RRM_hnRNPH_ESRPs_RBM12_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050666">
        <property type="entry name" value="ESRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13976">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="187"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="629"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="999"/>
            <end position="1075"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="156"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="281"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="564"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="841"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="875"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="281"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="317"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="367"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="377"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="443"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="569"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="594"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="612"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="879"/>
            <end position="900"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAP48572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000002125.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="1075" mass="120487" checksum="24A993DB6F4BCBD8" modified="2004-07-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-07" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>C4B8X2</accession>
    <accession>A0A1W2VVP6</accession>
    <accession>F7BNA6</accession>
    <name>C4B8X2_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Nucleoside diphosphate kinase homolog 5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">3'-5' exonuclease NME5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">Ci-NDK/DPY26</name>
        <name evidence="13" type="synonym">ci-ndk/dpy26</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2009" name="FEBS Lett." volume="583" first="2201" last="2207">
            <title>Proteomic characterization of sperm radial spokes identifies a novel spoke protein with an ubiquitin domain.</title>
            <authorList>
                <person name="Satouh Y."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="19527718"/>
            <dbReference type="DOI" id="10.1016/j.febslet.2009.06.016"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Testis</tissue>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8 9">Belongs to the NDK family.</text>
    </comment>
    <dbReference type="EMBL" id="AB501296">
        <property type="protein sequence ID" value="BAH59281.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001154961.1">
        <property type="nucleotide sequence ID" value="NM_001161489.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C4B8X2"/>
    <dbReference type="STRING" id="7719.ENSCINP00000008954"/>
    <dbReference type="Ensembl" id="ENSCINT00000008954.3">
        <property type="protein sequence ID" value="ENSCINP00000008954.3"/>
        <property type="gene ID" value="ENSCING00000004328.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100177987"/>
    <dbReference type="KEGG" id="cin:100177987"/>
    <dbReference type="CTD" id="100177987"/>
    <dbReference type="GeneTree" id="ENSGT00940000159595"/>
    <dbReference type="HOGENOM" id="CLU_060216_2_0_1"/>
    <dbReference type="OMA" id="HNAISYW"/>
    <dbReference type="OrthoDB" id="1729737at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004550">
        <property type="term" value="F:nucleoside diphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003341">
        <property type="term" value="P:cilium movement"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006241">
        <property type="term" value="P:CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006183">
        <property type="term" value="P:GTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902176">
        <property type="term" value="P:negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
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                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
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                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
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                <person name="Kobayashi K."/>
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                <person name="Makabe K.W."/>
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                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
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                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
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                <person name="Sasaki A."/>
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                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
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                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
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                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
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                <person name="Chapman J."/>
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                <person name="De Tomaso A."/>
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                <person name="Harafuji N."/>
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                <person name="Yamada L."/>
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                <person name="Awazu S."/>
                <person name="Azumi K."/>
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                <person name="Zhang C."/>
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                <person name="Doggett N."/>
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                <person name="Richardson P."/>
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                <person name="Wiley G.B."/>
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            <authorList>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Wiley G.B."/>
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                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01001740">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="ENSCINP00000020757.3"/>
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        <property type="component" value="Chromosome 3"/>
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        <location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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        <source>
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        <source>
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    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="252" mass="29697" checksum="5443AB0E7249F88D" modified="2011-07-27" version="1">MKIICSGMSKTGTKTVCQALRILGYEVYDFEEQFWYLGDRLIKCLDEGWTDDDLREMFKDVDAFTDIPSCLLWEDISRVFPDAKIIHMERNNEDEWARSFKNQLEKIYYNRILRTLLLLSPTGTKLRRYYSACNRVQASCQTLMPWKNIPINIPLARMRYRMHNVNVRQSASVPKDRIHFLKHSEGWKPLCAFLGVLVPDTPYPHRNKGGEIIQQLMDRSYVFVQIKKEVIFSLSLIVVALGFLVYFRHLLF</sequence>
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        <name evidence="1" type="primary">LOC100182917</name>
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        <name type="scientific">Ciona intestinalis</name>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <dbReference type="STRING" id="7719.ENSCINP00000004919"/>
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        <property type="protein sequence ID" value="ENSCINP00000004919.3"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="entry name" value="DNA_mismatch_repair_MutS_sf"/>
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        <property type="entry name" value="DNA repair protein MutS, domain III"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000004919.3"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="127" mass="14261" checksum="C2BB05DE6A5EE963" modified="2012-04-18" version="2">MTTAILLKHVTELVPALSDVLSHAKCELFVAYKVSLQDARYGCIVDIINRTIHEDARYTKGHLNMKTQRCFAVKQGLHGVLDLSRITYTELIEDINELIAQLGDKHGLPLKSAYTISRGFHIQLATK</sequence>
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    <accession>F6QW24</accession>
    <name>F6QW24_CIOIN</name>
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        <recommendedName>
            <fullName evidence="6">Metalloendopeptidase</fullName>
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    <gene>
        <name evidence="10" type="primary">LOC100185524</name>
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    <organism evidence="10 11">
        <name type="scientific">Ciona intestinalis</name>
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        <lineage>
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    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
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                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
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                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">wild type</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Metalloprotease.</text>
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    <comment type="cofactor">
        <cofactor evidence="5 6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="5 6">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="Ensembl" id="ENSCINT00000013536.3">
        <property type="protein sequence ID" value="ENSCINP00000013536.3"/>
        <property type="gene ID" value="ENSCING00000006594.3"/>
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        <property type="component" value="Chromosome 9"/>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="ZnMc_astacin_like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Antistasin, domain 1"/>
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        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Antistasin-like"/>
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        <property type="entry name" value="Astacin-like_dom"/>
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        <property type="entry name" value="Hirudin/antistatin"/>
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        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
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        <property type="entry name" value="Peptidase_M12A"/>
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        <property type="entry name" value="Peptidase_Metallo"/>
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        <property type="entry name" value="ShKT_dom"/>
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        <property type="entry name" value="DISCOIDIN, CUB, EGF, LAMININ , AND ZINC METALLOPROTEASE DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="METALLOENDOPEPTIDASE"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Astacin"/>
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        <property type="entry name" value="ASTACIN"/>
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        <property type="entry name" value="Leech antihemostatic proteins"/>
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        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
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    <keyword evidence="3 5 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 6" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2 5 6" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 6" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5005129886" description="Metalloendopeptidase" evidence="6">
        <location>
            <begin position="21"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="domain" description="Antistasin-like" evidence="7">
        <location>
            <begin position="65"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M12A" evidence="9">
        <location>
            <begin position="101"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="ShKT" evidence="8">
        <location>
            <begin position="349"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="203"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
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    </feature>
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        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
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        </ligand>
    </feature>
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        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01005"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51252"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51864"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000013536.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="391" mass="43911" checksum="E8AFB8C62F645536" modified="2012-04-18" version="2">MLFTKELSFAMVLLVHLVQAVPVVNLVNMPDTELCALIRNGTNVAFAMEEAEKCNDTQDDLGAGCPSVECDNSLLCSNGYLLDAEGCETCECIEWARDMEMDITGDFDDIDVWKDYTDELGEFVITYETSEYLTEKKAITQAIDEINENTCLQFLPRKINDSKENYIRFEWDEVCSSAVGKTGGAQGVILSEECSTKGGVLHELMHTLGFLHEHNRPDRDQYINIEWENIIPEKRSMFNKWQVSEDGVLNEFPYDVSSVTHLDSFAFSNNGEPTITGLDGFPLPTQNTHLSVLDIDKINHVYGCPEFMPLPPMMPDKCKDDLFACPELANTCACLLSPAFMMNHCCSSCEMEATQIVDHFPFCNLWAANCGKSRYFDNLCRKTCPKCVLAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>F6QYI8</accession>
    <name>F6QYI8_CIOIN</name>
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        <recommendedName>
            <fullName evidence="8">Sulfatase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">wild type</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sulfatase family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002006">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="ENSCING00000008867.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166538"/>
    <dbReference type="HOGENOM" id="CLU_006332_3_0_1"/>
    <dbReference type="InParanoid" id="F6QYI8"/>
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    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:sulfuric ester hydrolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047115">
        <property type="entry name" value="ARSB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024607">
        <property type="entry name" value="Sulfatase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000917">
        <property type="entry name" value="Sulfatase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10342">
        <property type="entry name" value="ARYLSULFATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10342:SF274">
        <property type="entry name" value="ARYLSULFATASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00884">
        <property type="entry name" value="Sulfatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SULFATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00149">
        <property type="entry name" value="SULFATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003345336" description="Sulfatase N-terminal domain-containing protein" evidence="7">
        <location>
            <begin position="27"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Sulfatase N-terminal" evidence="8">
        <location>
            <begin position="34"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00884"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000018053.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="244" mass="28200" checksum="8454A5C8CAA63C1E" modified="2012-04-18" version="2">MFSGPKHLNAVRLLAFVFWLSAVACGKKQRGQRPHVVFVLADDFGFNDIGYHAREHYSDMYTPFLDSLAAKGVILENYYVQPICSPTRGQLLTGRYQIHTGLAHGIIRAAQPYGLPLDNILLSQQLRQCGYKTNMVGKWHLGFFREEYLPWNRGFQNFFGFLNGGVNHFTRYHCEPKKTRRFCGYDMIDSRYGPTNATYGEYSTNLFIRKSKEMIDKHNKQKPMFLYLSLQAVHGPLQVPNQYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>F6R5U5</accession>
    <name>F6R5U5_CIOIN</name>
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        <recommendedName>
            <fullName evidence="4">Sushi domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">wild type</strain>
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        <scope>IDENTIFICATION</scope>
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            <authorList>
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        <scope>IDENTIFICATION</scope>
    </reference>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">wild type</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01000080">
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome 1"/>
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    <evidence type="ECO:0000313" key="1">
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            <dbReference type="Ensembl" id="ENSCINP00000025163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="238" mass="26959" checksum="339B394FE99DC6C0" modified="2011-07-27" version="1">MVKNCSPNLLANELPRCSSLWTESRTFYLGADVNTIPRPRQNSSLSRRTDIGILKSKANNDMFTYGCDTEATCKTTANSNSPHSFWIRSVSVSSGFPTPRFSKHNEVPRRKRDWILVQCVTKYTSRIICSAEPMPKLSYHEIGCQEMVLCPAMLRSVEYIEDVCCQHFNKSSKEETLQSGRTVELLFSAKGPVVRDDKHLTGRSIFYFCFDEPLENRRRLCADCVAARAAVPTPTNDN</sequence>
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    <accession>F6RM44</accession>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
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                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
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                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
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                <person name="Kawashima T."/>
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                <person name="Putnam N."/>
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                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
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                <person name="Kobayashi M."/>
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                <person name="Manohar C."/>
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                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
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                <person name="Satoh N."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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        <text evidence="1">Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.</text>
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    <proteinExistence type="inferred from homology"/>
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            <begin position="5"/>
            <end position="22"/>
        </location>
    </feature>
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        <source>
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    </evidence>
    <evidence type="ECO:0000259" key="2">
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
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            <fullName evidence="10">EGF-like domain-containing protein</fullName>
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    <reference evidence="12" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
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                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
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                <person name="Putnam N."/>
                <person name="Rash S."/>
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                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
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    <dbReference type="AlphaFoldDB" id="F6RW80"/>
    <dbReference type="STRING" id="7719.ENSCINP00000015784"/>
    <dbReference type="Ensembl" id="ENSCINT00000015784.3">
        <property type="protein sequence ID" value="ENSCINP00000015784.3"/>
        <property type="gene ID" value="ENSCING00000021832.1"/>
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    <dbReference type="GeneTree" id="ENSGT00950000183158"/>
    <dbReference type="HOGENOM" id="CLU_416145_0_0_1"/>
    <dbReference type="InParanoid" id="F6RW80"/>
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    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="606"/>
            <end position="626"/>
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        <location>
            <begin position="76"/>
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    <feature type="domain" description="EGF-like" evidence="10">
        <location>
            <begin position="117"/>
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    <feature type="domain" description="EGF-like" evidence="10">
        <location>
            <begin position="157"/>
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    <feature type="domain" description="EGF-like" evidence="10">
        <location>
            <begin position="198"/>
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    <feature type="domain" description="EGF-like" evidence="10">
        <location>
            <begin position="239"/>
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    <feature type="domain" description="EGF-like" evidence="10">
        <location>
            <begin position="280"/>
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    </feature>
    <feature type="domain" description="EGF-like" evidence="10">
        <location>
            <begin position="325"/>
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    <feature type="domain" description="EGF-like" evidence="10">
        <location>
            <begin position="370"/>
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    <feature type="domain" description="EGF-like" evidence="10">
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        <location>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
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                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
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                <person name="Shoguchi E."/>
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                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
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                <person name="Yoshizaki F."/>
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                <person name="Zhang C."/>
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                <person name="Detter C."/>
                <person name="Doggett N."/>
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                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <text evidence="1">Belongs to the recoverin family.</text>
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    <keyword evidence="2" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0519">Myristate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="60"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="96"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="143"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000027731.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="192" mass="21762" checksum="865E04A195B43F2C" modified="2012-04-18" version="2">MGQQHPSLKPRMLDDLMRMSDFSADELKIWYNEFSKDSVSGFLSKNDFIKIYKGLFPHGDASGFADHVFRTFDANKDGVLNFREFVIGLSLTMKGPLDDKLYWAFKLYDVDGNGFVTKDEMNEIIAVSNAIFTVCCTDTTDDDVIMSGREAFKTMDTDEDGKVSWGEFRKGIHSNRVFMLIVEAKMKNAGVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>F6SK76</accession>
    <name>F6SK76_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="F6SK76"/>
    <dbReference type="Ensembl" id="ENSCINT00000022855.2">
        <property type="protein sequence ID" value="ENSCINP00000022609.2"/>
        <property type="gene ID" value="ENSCING00000011972.2"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3224264_0_0_1"/>
    <dbReference type="InParanoid" id="F6SK76"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000022609.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="44" mass="5047" checksum="0B1169EE1C9B5A79" modified="2012-04-18" version="2">MVNLILSAIRQSSVRISVKLKQKQLFQIEAMKEELKCGLRLICS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>F6SRD6</accession>
    <name>F6SRD6_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">Alpha-tubulin N-acetyltransferase</fullName>
            <shortName evidence="6">Alpha-TAT</shortName>
            <shortName evidence="6">TAT</shortName>
            <ecNumber evidence="5 6">2.3.1.108</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Acetyltransferase mec-17 homolog</fullName>
        </alternativeName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">wild type</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Specifically acetylates 'Lys-40' in alpha-tubulin on the lumenal side of microtubules. Promotes microtubule destabilization and accelerates microtubule dynamics; this activity may be independent of acetylation activity. Acetylates alpha-tubulin with a slow enzymatic rate, due to a catalytic site that is not optimized for acetyl transfer. Enters the microtubule through each end and diffuses quickly throughout the lumen of microtubules. Acetylates only long/old microtubules because of its slow acetylation rate since it does not have time to act on dynamically unstable microtubules before the enzyme is released. Required for normal sperm flagellar function. Promotes directional cell locomotion and chemotaxis, through AP2A2-dependent acetylation of alpha-tubulin at clathrin-coated pits that are concentrated at the leading edge of migrating cells. May facilitate primary cilium assembly.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 6">
            <text>L-lysyl-[alpha-tubulin] + acetyl-CoA = N(6)-acetyl-L-lysyl-[alpha-tubulin] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15277"/>
            <dbReference type="Rhea" id="RHEA-COMP:11278"/>
            <dbReference type="Rhea" id="RHEA-COMP:11279"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="2.3.1.108"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the acetyltransferase ATAT1 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.108" evidence="5 6"/>
    <dbReference type="EMBL" id="EAAA01002889">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6SRD6"/>
    <dbReference type="STRING" id="7719.ENSCINP00000025666"/>
    <dbReference type="Ensembl" id="ENSCINT00000025912.2">
        <property type="protein sequence ID" value="ENSCINP00000025666.2"/>
        <property type="gene ID" value="ENSCING00000014113.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000008276"/>
    <dbReference type="HOGENOM" id="CLU_025013_2_1_1"/>
    <dbReference type="InParanoid" id="F6SRD6"/>
    <dbReference type="OMA" id="FFIGRHP"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019799">
        <property type="term" value="F:tubulin N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048666">
        <property type="term" value="P:neuron development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070507">
        <property type="term" value="P:regulation of microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000060">
        <property type="entry name" value="Alpha-tubulin N-acetyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03130">
        <property type="entry name" value="mec17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038746">
        <property type="entry name" value="Atat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007965">
        <property type="entry name" value="GNAT_ATAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12327">
        <property type="entry name" value="ALPHA-TUBULIN N-ACETYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12327:SF0">
        <property type="entry name" value="ALPHA-TUBULIN N-ACETYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05301">
        <property type="entry name" value="Acetyltransf_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51730">
        <property type="entry name" value="GNAT_ATAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="7">
        <location>
            <begin position="1"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="118"/>
            <end position="131"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="154"/>
            <end position="163"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="site" description="Crucial for catalytic activity" evidence="6">
        <location>
            <position position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00051998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00054822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00066570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000025666.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="184" mass="21013" checksum="221B952F0F5B2965" modified="2012-04-18" version="2">MEFDFNINHLFPDKITLVGENSSYRKHANSKILQRNLQIVIEVLGQRSARAQQLTGSITTLLKSQLNNQRIYVLKEANANNSGLGCVIGFLKTGKKRLFVLDRDGNHNEMNPLCVLDFYVHESQQRKGCGLCLFKHMLHVEGVKASHLAIDRPSHKFISFLKKHFSLWATVPQVNNFVIFDGFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>F6SSH7</accession>
    <name>F6SSH7_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">START domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01002651">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6SSH7"/>
    <dbReference type="STRING" id="7719.ENSCINP00000004446"/>
    <dbReference type="Ensembl" id="ENSCINT00000004446.3">
        <property type="protein sequence ID" value="ENSCINP00000004446.3"/>
        <property type="gene ID" value="ENSCING00000002174.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157856"/>
    <dbReference type="HOGENOM" id="CLU_062829_0_0_1"/>
    <dbReference type="InParanoid" id="F6SSH7"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002913">
        <property type="entry name" value="START_lipid-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051213">
        <property type="entry name" value="START_lipid_transfer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19308">
        <property type="entry name" value="PHOSPHATIDYLCHOLINE TRANSFER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19308:SF8">
        <property type="entry name" value="STAR-RELATED LIPID TRANSFER PROTEIN 7, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01852">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50848">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="START" evidence="1">
        <location>
            <begin position="157"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000004446.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="251" mass="30264" checksum="E5844ADDA9404069" modified="2012-04-18" version="2">MTSFNPSSWKNILTIYRNPRLKLLHQQCKGQLRQLHLTSKCNQYSQKGNEKSSFFQLLFNNLKQHMNKAKSRHQNRWQYYWSSQSRLAESLYHYLYPKRPVYFIAGAACSFSWDKNGITDAELDEHVNELYMLKQDPVDNQSCVNANEQEFNTPSYWEMILDKPDIKIWRLPYKDTEFMQYKVFGRFKDVTARQFFTIQVDDIYRKKWDDHVLSINVLESNPKNGEKVVQWVSHFPYPFNPREYVYVRRAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>F6SV73</accession>
    <name>F6SV73_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">tRNA methyltransferase 10 homolog B</fullName>
            <ecNumber evidence="1">2.1.1.221</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">RNA (guanine-9-)-methyltransferase domain-containing protein 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">tRNA (guanine(9)-N(1))-methyltransferase TRMT10B</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">wild type</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">S-adenosyl-L-methionine-dependent guanine N(1)-methyltransferase that catalyzes the formation of N(1)-methylguanine at position 9 (m1G9) in tRNAs. Probably not able to catalyze formation of N(1)-methyladenine at position 9 (m1A9) in tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>guanosine(9) in tRNA + S-adenosyl-L-methionine = N(1)-methylguanosine(9) in tRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43156"/>
            <dbReference type="Rhea" id="RHEA-COMP:10367"/>
            <dbReference type="Rhea" id="RHEA-COMP:10368"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73542"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="EC" id="2.1.1.221"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.1.1.221" evidence="1"/>
    <dbReference type="EMBL" id="EAAA01001120">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6SV73"/>
    <dbReference type="SMR" id="F6SV73"/>
    <dbReference type="FunCoup" id="F6SV73">
        <property type="interactions" value="833"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000014945"/>
    <dbReference type="Ensembl" id="ENSCINT00000014945.3">
        <property type="protein sequence ID" value="ENSCINP00000014945.3"/>
        <property type="gene ID" value="ENSCING00000007283.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063169"/>
    <dbReference type="HOGENOM" id="CLU_034384_8_0_1"/>
    <dbReference type="InParanoid" id="F6SV73"/>
    <dbReference type="OMA" id="ALQAWFP"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052905">
        <property type="term" value="F:tRNA (guanosine(9)-N1)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002939">
        <property type="term" value="P:tRNA N1-guanine methylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18100">
        <property type="entry name" value="Trm10euk_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1280.30:FF:000013">
        <property type="entry name" value="tRNA methyltransferase 10 homolog B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028564">
        <property type="entry name" value="MT_TRM10-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038459">
        <property type="entry name" value="MT_TRM10-typ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047911">
        <property type="entry name" value="Trm10_B_MTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007356">
        <property type="entry name" value="tRNA_m1G_MeTrfase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13563">
        <property type="entry name" value="TRNA (GUANINE-9-) METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13563:SF19">
        <property type="entry name" value="TRNA METHYLTRANSFERASE 10 HOMOLOG B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51675">
        <property type="entry name" value="SAM_MT_TRM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM-dependent MTase TRM10-type" evidence="12">
        <location>
            <begin position="52"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="14"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="27"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00035725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00045240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000014945.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="263" mass="30548" checksum="FA14B7A7FDDF2A3B" modified="2012-04-18" version="2">KNEMRRLLKYERKVESKRIKRKKEHERRREREKAMEKASEIQLSRFARSNLKRREVDEKMESVMRNDSHALVVCVDLSWSHSMNDKEINKLASQLGRLYGSNRKAEAPAHVYFTGFKKGTLIWNECHRKHNGFNEYKISMTDKSPIEMFNNENIIYLSPDAETALSNVEAGSVYVIGGLVDETVCKKLTLDAATEHGICTRRLPVKEFMKKSGRHKNYSTVFAVNQVFDILLKYHSAKDWVEAFKLGVPPRKGYVFNDDVITS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>F6SZF0</accession>
    <name>F6SZF0_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ATPase dynein-related AAA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">wild type</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01000952">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6SZF0"/>
    <dbReference type="STRING" id="7719.ENSCINP00000014301"/>
    <dbReference type="Ensembl" id="ENSCINT00000014301.3">
        <property type="protein sequence ID" value="ENSCINP00000014301.3"/>
        <property type="gene ID" value="ENSCING00000022123.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00550000074802"/>
    <dbReference type="HOGENOM" id="CLU_1844393_0_0_1"/>
    <dbReference type="InParanoid" id="F6SZF0"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:005027">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011704">
        <property type="entry name" value="ATPase_dyneun-rel_AAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48103:SF2">
        <property type="entry name" value="MIDASIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48103">
        <property type="entry name" value="MIDASIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07728">
        <property type="entry name" value="AAA_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATPase dynein-related AAA" evidence="3">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000014301.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="139" mass="15986" checksum="825F09E213630668" modified="2011-07-27" version="1">MKKGHWVVLDELNLASQSVLESLNSVFDHRGELYVPELARSFLVNRDQTKIFACQNPVHEGGGRRGLPKSFLNRFTQVYVRGLAGEDMEFILNNSFPQIPPDVMKLMVKFNQQVNTSHCRVMKTPHAYCRVMYTPHAYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>F6SZZ3</accession>
    <name>F6SZZ3_CIOIN</name>
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        <recommendedName>
            <fullName evidence="4">MIF4G domain-containing protein</fullName>
        </recommendedName>
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    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01002336">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6SZZ3"/>
    <dbReference type="STRING" id="7719.ENSCINP00000005880"/>
    <dbReference type="Ensembl" id="ENSCINT00000005880.3">
        <property type="protein sequence ID" value="ENSCINP00000005880.3"/>
        <property type="gene ID" value="ENSCING00000018231.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000064318"/>
    <dbReference type="HOGENOM" id="CLU_122187_0_0_1"/>
    <dbReference type="InParanoid" id="F6SZZ3"/>
    <dbReference type="OMA" id="MRYQEFT"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.180:FF:000225">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039762">
        <property type="entry name" value="Nmd2/UPF2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12839">
        <property type="entry name" value="NONSENSE-MEDIATED MRNA DECAY PROTEIN 2 UP-FRAMESHIFT SUPPRESSOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12839:SF7">
        <property type="entry name" value="REGULATOR OF NONSENSE TRANSCRIPTS 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="8"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000005880.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
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        <name type="scientific">Ciona intestinalis</name>
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            <taxon>Eukaryota</taxon>
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        </lineage>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">wild type</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01001703">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6TJS8"/>
    <dbReference type="STRING" id="7719.ENSCINP00000019460"/>
    <dbReference type="Ensembl" id="ENSCINT00000019460.3">
        <property type="protein sequence ID" value="ENSCINP00000019460.3"/>
        <property type="gene ID" value="ENSCING00000009569.3"/>
    </dbReference>
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    <dbReference type="HOGENOM" id="CLU_002818_0_0_1"/>
    <dbReference type="InParanoid" id="F6TJS8"/>
    <dbReference type="OMA" id="GTESMGH"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="CDD" id="cd07354">
        <property type="entry name" value="HN_L-delphilin-R1_like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PDZ1_L-delphilin-like"/>
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    </dbReference>
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        <property type="entry name" value="PDZ2_L-delphilin-like"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Formin_Homology"/>
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        <property type="entry name" value="PDZ"/>
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        <property type="entry name" value="PDZ_6"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000019460.3"/>
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        <source>
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        </source>
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</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>F6TN93</accession>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    <dbReference type="Ensembl" id="ENSCINT00000005809.3">
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        <property type="gene ID" value="ENSCING00000002851.3"/>
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    <dbReference type="InParanoid" id="F6TN93"/>
    <dbReference type="OMA" id="LAGSQWI"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000005809.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="199" mass="23543" checksum="758BE45B20A0000E" modified="2012-04-18" version="2">MSVVNKYRRLIVTQQNLSRLISTCSREYARRILRMDKSYTNLQKMKQIKYPQDYIEEKVVEYKLGTRRLAVMMGQDETGFDDRKIKEALKYLFPTDLSDRKTRPTMEHPNKIFPAQLKPQADPTGRPYHPLFYTGHPQFYDLMHDVYNHLLQLQDVRRKATKMGKSPHEESVNLQSSKWISKSKLESNFLESIPISDEN</sequence>
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<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
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    <name>F6TT57_CIOIN</name>
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        <recommendedName>
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    <organism evidence="10 11">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
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    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <reaction evidence="6">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1-acyl-sn-glycero-3-phosphocholine + a fatty acid + H(+)</text>
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        <property type="term" value="F:long-chain fatty acyl-CoA hydrolase activity"/>
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        <property type="entry name" value="Patatin"/>
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    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
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        <source>
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<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
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    <reference evidence="19" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">wild type</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Complex I is composed of 45 different subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the complex I NDUFB6 subunit family.</text>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="ENSCING00000012183.2"/>
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        <property type="component" value="Chromosome 5"/>
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    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0045271">
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="P:mitochondrial ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0006120">
        <property type="term" value="P:mitochondrial electron transport, NADH to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="entry name" value="NADH_DH_b-subcmplx_su6"/>
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        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 BETA SUBCOMPLEX SUBUNIT 6"/>
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        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 BETA SUBCOMPLEX SUBUNIT 6"/>
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    <keyword evidence="10" id="KW-0249">Electron transport</keyword>
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    <keyword evidence="9" id="KW-0999">Mitochondrion inner membrane</keyword>
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    <keyword evidence="7" id="KW-0679">Respiratory chain</keyword>
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    <keyword evidence="11 17" id="KW-1133">Transmembrane helix</keyword>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="13" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="13">wild type</strain>
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    <reference evidence="13" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
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                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_026694151.1">
        <property type="nucleotide sequence ID" value="XM_026838350.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_026694152.1">
        <property type="nucleotide sequence ID" value="XM_026838351.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6U7B2"/>
    <dbReference type="STRING" id="7719.ENSCINP00000026887"/>
    <dbReference type="Ensembl" id="ENSCINT00000027133.2">
        <property type="protein sequence ID" value="ENSCINP00000026887.2"/>
        <property type="gene ID" value="ENSCING00000007055.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100179720"/>
    <dbReference type="GeneTree" id="ENSGT00940000156904"/>
    <dbReference type="HOGENOM" id="CLU_532022_0_0_1"/>
    <dbReference type="InParanoid" id="F6U7B2"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000125">
        <property type="entry name" value="Putative zinc finger protein 143"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000233">
        <property type="entry name" value="Putative zinc finger protein 362"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000012">
        <property type="entry name" value="RB-associated KRAB zinc finger protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23226:SF416">
        <property type="entry name" value="FI01424P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23226">
        <property type="entry name" value="ZINC FINGER AND SCAN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="106"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="136"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="164"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="192"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="227"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="39"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="247"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="469"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="48"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="247"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="473"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000026887.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="512" mass="56217" checksum="568770CD5039FB11" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>F6U9P7</accession>
    <name>F6U9P7_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">tRNA-queuosine alpha-mannosyltransferase</fullName>
            <ecNumber evidence="4">2.4.1.110</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Glycosyltransferase-like domain-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Glycosyltransferase that specifically catalyzes mannosylation of cytoplasmic tRNA(Asp) modified with queuosine at position 34 (queuosine(34)). Mannosylates the cyclopentene moiety of queuosine(34) in tRNA(Asp) to form mannosyl-queuosine(34). Mannosylation of queuosine(34) in tRNA(Asp) is required to slow-down elongation at cognate codons, GAC and GAU, thereby regulating protein translation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>queuosine(34) in tRNA(Asp) + GDP-alpha-D-mannose = O-4''-alpha-D-mannosylqueuosine(34) in tRNA(Asp) + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12885"/>
            <dbReference type="Rhea" id="RHEA-COMP:18572"/>
            <dbReference type="Rhea" id="RHEA-COMP:18581"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57527"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:194431"/>
            <dbReference type="ChEBI" id="CHEBI:194442"/>
            <dbReference type="EC" id="2.4.1.110"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:12886"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 4 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.110" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="F6U9P7"/>
    <dbReference type="FunCoup" id="F6U9P7">
        <property type="interactions" value="74"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000021446"/>
    <dbReference type="Ensembl" id="ENSCINT00000021446.3">
        <property type="protein sequence ID" value="ENSCINP00000021446.3"/>
        <property type="gene ID" value="ENSCING00000001951.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000006631"/>
    <dbReference type="HOGENOM" id="CLU_033439_1_0_1"/>
    <dbReference type="InParanoid" id="F6U9P7"/>
    <dbReference type="OMA" id="CYPIAPN"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016438">
        <property type="term" value="F:tRNA-queuosine(34) beta-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd01635">
        <property type="entry name" value="Glycosyltransferase_GTB-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051862">
        <property type="entry name" value="GT-like_domain_containing_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022701">
        <property type="entry name" value="QTMAN_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13615">
        <property type="entry name" value="GLYCOSYLTRANSFERASE-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13615:SF3">
        <property type="entry name" value="GLYCOSYLTRANSFERASE-LIKE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12038">
        <property type="entry name" value="QTMAN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="tRNA-queuosine alpha-mannosyltransferase N-terminal" evidence="10">
        <location>
            <begin position="6"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="9">
        <location>
            <begin position="194"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00044517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00044539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00045402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF12038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000021446.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="370" mass="42962" checksum="31B403ACE6C3DC5A" modified="2012-04-18" version="2">LDCVDILIVESFYGGSHKQLIDVIVEAVKEKHGNLNLKLLTLPAKKWPWKARTSALYFSTLIDSALTCKTIFCSSVLNLCELVGLHPQLGKAKKVLYFHENQLVYPVRNRHDGDFQFGYNQIMSALAADFVLFNSSFNMESFLTNIQSFLKLIPDHKPKNIEGKLRPKCKVLYFPIPNIPRVILESDELNELHIVWPHRWEHDKDPDTFFQVLFLLKENDFKFKVSVLGEHFAEIPPIFQTAKEKLGEKFVNHWGYLEAKSLYLNLLQRCDVVVSTAVHEFFGVSMLEAVTAGCYPLCPNRLSYPEIFPAECLYNTQQQLYKKLKEFCKHKHLPKQLYNKLNFNVKRFQWQNLESKYLAMLGEHCVISQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F6UEC9</accession>
    <name>F6UEC9_CIOIN</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
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            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="F6UEC9"/>
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        <property type="protein sequence ID" value="ENSCINP00000005266.3"/>
        <property type="gene ID" value="ENSCING00000002587.3"/>
    </dbReference>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.220.30">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000005266.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="251" mass="29086" checksum="6BC0F09A9918A473" modified="2012-04-18" version="2">IYFQSKYLETPKLQCEPASLKFKEQVTIKLQTHVVLDKETVRRCKLLHSEDGITWEETKTKLEFSDNFISFQTNHFSWWKVIFEIIEKSVAFLYKVQLNFLLYQNAGAVVWKVCWNLDLATKESILQENKFSQETWIVPGGNDLILYIAPRDENPNVGIIPSKITIPADQINKSCHFDRRFEVQGQFSSRIWLKAKRDCGNLHEEEPIWFPLPNTQGQQPTNQIVNMVGNKLDHAVVLGKDVNIQSSSSKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>F6UKF6</accession>
    <name>F6UKF6_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">wild type</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BCOR family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002404">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6UKF6"/>
    <dbReference type="STRING" id="7719.ENSCINP00000022589"/>
    <dbReference type="Ensembl" id="ENSCINT00000022835.2">
        <property type="protein sequence ID" value="ENSCINP00000022589.2"/>
        <property type="gene ID" value="ENSCING00000011957.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153737"/>
    <dbReference type="HOGENOM" id="CLU_586532_0_0_1"/>
    <dbReference type="InParanoid" id="F6UKF6"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="CDD" id="cd14259">
        <property type="entry name" value="PUFD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.260.40">
        <property type="entry name" value="BCL-6 corepressor, PCGF1 binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ankyrin_rpt"/>
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        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047144">
        <property type="entry name" value="BCOR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038227">
        <property type="entry name" value="PUFD_som_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24117">
        <property type="entry name" value="AGAP007537-PB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24117:SF9">
        <property type="entry name" value="BCL-6 COREPRESSOR PCGF1 BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ANK" evidence="2">
        <location>
            <begin position="20"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="2">
        <location>
            <begin position="53"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="360"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="426"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="426"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="448"/>
            <end position="466"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00034703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000022589.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="466" mass="52309" checksum="2F8E87B59374A488" modified="2012-04-18" version="2">MENMKHYLESGFSVNAKDNAGYTALHEACVQGRLEAAKLLLQFGADVNLNSLDGTRPIHDAVEYDHIEMTRLMMSCGADPLLSKFSGRSIKNMIRSTQMGNFINAYLKELQPVCEELMEDSDMKWKIPFSSVLESKQDQGYDLMGNPPAEDTQAHCMEFELFDEQPIDTYLVQPSDTTDRSNYYLLNDILQVKGCSKADFLLEHCCNIRTMPTNKFCSEADLNKFGKRNRNFNETNSLIELVAADKPLTRHVGLRVHPTGEVEEIPQFKKQKSSRLPPPVANDNVVKFLPKTTDTPTCSETPDQTRSNSNLLENLSDSVIDDHREESLACLQKLTASFCMTGHIGKEYKDFGKVNGIHTPGTQTPVKKPRPPAMEVNLPKTSTPNIEKNHKQNSDHIPMECLPPYNHHIEPKQPPKLPPLAPINTDSGLRSLPTQQNGVDFSPFNKLPTFTESQNPVNTPNLVPEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F6ULE7</accession>
    <accession>A0A1W2WJM4</accession>
    <name>F6ULE7_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EGF-like domain-containing protein</fullName>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
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            <taxon>Ciona</taxon>
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    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
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        <source>
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        <source>
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    </evidence>
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        <source>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
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                <person name="Doyle S."/>
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                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    <sequence length="281" mass="30490" checksum="897B74A446333DE2" modified="2012-04-18" version="2">METNVSVMQGEESFISPYNSPEKPDISRSLDGAGEPILSPGYSNVIEDIYEHGEGASTNGIDNHNDHFAAGDQIDIENITIHKPLNNHEVPDHSAGDTSIAVLPDEKVQPSLTTATEVIDNHTVNTATLDTEVPDHSAGDNSPAVLPDEKVQSSLNTAAEVIDNHTVNTANGNLPNNSTENTFIVMSSSENKENVENSEQSIEDMIASSPREMFEDMSSSEMMSVSQKMEVEFKEMLLQEKMLKKRSSDAGVEGKGATKQGCNIQVTTTTNETRKKAKDPK</sequence>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Suzuki M.M."/>
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                <person name="Yoshizaki F."/>
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                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
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                <person name="Richardson P."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <feature type="transmembrane region" description="Helical" evidence="2">
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        <source>
            <dbReference type="ARBA" id="ARBA00009024"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
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    </evidence>
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        <source>
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</entry>
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        <lineage>
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    <reference evidence="15" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
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                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
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                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
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                <person name="Kobayashi M."/>
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                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
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                <person name="Mochizuki Y."/>
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                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">wild type</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.5"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.5" evidence="1"/>
    <dbReference type="EMBL" id="EAAA01001088">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="F6VF77">
        <property type="interactions" value="130"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000004680"/>
    <dbReference type="Ensembl" id="ENSCINT00000004680.3">
        <property type="protein sequence ID" value="ENSCINP00000004680.3"/>
        <property type="gene ID" value="ENSCING00000002282.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166903"/>
    <dbReference type="HOGENOM" id="CLU_008964_5_0_1"/>
    <dbReference type="InParanoid" id="F6VF77"/>
    <dbReference type="OMA" id="YPERVGX"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
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    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
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        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0016185">
        <property type="term" value="P:synaptic vesicle budding from presynaptic endocytic zone membrane"/>
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        <property type="project" value="GO_Central"/>
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    <dbReference type="CDD" id="cd08771">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01256">
        <property type="entry name" value="PH_dynamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000045">
        <property type="entry name" value="dynamin-1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1240">
        <property type="entry name" value="Dynamin, middle domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022812">
        <property type="entry name" value="Dynamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001401">
        <property type="entry name" value="Dynamin_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019762">
        <property type="entry name" value="Dynamin_GTPase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045063">
        <property type="entry name" value="Dynamin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000375">
        <property type="entry name" value="Dynamin_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030381">
        <property type="entry name" value="G_DYNAMIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003130">
        <property type="entry name" value="GED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020850">
        <property type="entry name" value="GED_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
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    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566:SF212">
        <property type="entry name" value="DYNAMIN"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF01031">
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        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00350">
        <property type="entry name" value="Dynamin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02212">
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    </dbReference>
    <dbReference type="Pfam" id="PF00169">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00195">
        <property type="entry name" value="DYNAMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00053">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00302">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00410">
        <property type="entry name" value="G_DYNAMIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51718">
        <property type="entry name" value="G_DYNAMIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51388">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7" id="KW-0505">Motor protein</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="26"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="11">
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            <begin position="525"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="domain" description="GED" evidence="12">
        <location>
            <begin position="659"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="774"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="810"/>
            <end position="846"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011980"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
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    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048040"/>
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    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003932"/>
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    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS51718"/>
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    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000004680.3"/>
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    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>F6VKX1</accession>
    <name>F6VKX1_CIOIN</name>
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        <recommendedName>
            <fullName evidence="10">Uridine-cytidine kinase</fullName>
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        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">wild type</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 10">
            <text>cytidine + ATP = CMP + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24674"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17562"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.48"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>uridine + ATP = UMP + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16825"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16704"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.48"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Pyrimidine metabolism; CTP biosynthesis via salvage pathway; CTP from cytidine: step 1/3.</text>
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        <text evidence="1 10">Pyrimidine metabolism; UMP biosynthesis via salvage pathway; UMP from uridine: step 1/1.</text>
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        <source>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005408"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00047436"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00048909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00485"/>
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    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000014825.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="243" mass="27382" checksum="B96AFF59E44EB4AF" modified="2012-04-18" version="2">TIMSVGCKCAAIMPGSETNRERPFIIGVSGGTSSGKTSVCQKIIELLGESSAVNGARKVAIISQDNFYKSLNAEEIRLANNCQYNFDHPDAFDTKLMKATILDIANGRTIKIPDYDFKTHTRRKEFCEELHKCDVVLFEGILVFYHKEIRELFNMKLFVDSDADTRLSRRVLRDITDRGRTLESVLQQYTTFVKPAFEEFCLPSKKYADVIIPRGAENLVAINLIVQHIRDILNGGIIKRANG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>F6VLB0</accession>
    <name>F6VLB0_CIOIN</name>
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        <recommendedName>
            <fullName evidence="2">VWFA domain-containing protein</fullName>
        </recommendedName>
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    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Tunicata</taxon>
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            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01001164">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6VLB0"/>
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    <dbReference type="Ensembl" id="ENSCINT00000026832.2">
        <property type="protein sequence ID" value="ENSCINP00000026586.2"/>
        <property type="gene ID" value="ENSCING00000014797.2"/>
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    <dbReference type="OMA" id="VIMDWWY"/>
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        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="CDD" id="cd00198">
        <property type="entry name" value="vWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
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    </dbReference>
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        <property type="entry name" value="DUF4537"/>
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        <property type="entry name" value="VWF_A"/>
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        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46785">
        <property type="entry name" value="VON WILLEBRAND FACTOR A DOMAIN-CONTAINING PROTEIN 3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46785:SF1">
        <property type="entry name" value="VON WILLEBRAND FACTOR A DOMAIN-CONTAINING PROTEIN 3B"/>
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        <property type="entry name" value="DUF4537"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
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        <location>
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        <location>
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            <end position="781"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="765"/>
            <end position="778"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000026586.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="1177" mass="134181" checksum="C558AB30687DA6B8" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>F6W5A6</accession>
    <accession>A0A1W2WDH1</accession>
    <name>F6W5A6_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein lin-37 homolog</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="primary">LOC100182887</name>
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    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
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            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
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                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_002127198.1">
        <property type="nucleotide sequence ID" value="XM_002127162.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_026696185.1">
        <property type="nucleotide sequence ID" value="XM_026840384.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6W5A6"/>
    <dbReference type="FunCoup" id="F6W5A6">
        <property type="interactions" value="11"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000004369"/>
    <dbReference type="Ensembl" id="ENSCINT00000004369.3">
        <property type="protein sequence ID" value="ENSCINP00000004369.3"/>
        <property type="gene ID" value="ENSCING00000002142.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100182887"/>
    <dbReference type="KEGG" id="cin:100182887"/>
    <dbReference type="GeneTree" id="ENSGT00390000002748"/>
    <dbReference type="HOGENOM" id="CLU_090128_0_0_1"/>
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    <dbReference type="OrthoDB" id="6287771at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
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    <dbReference type="GO" id="GO:0031523">
        <property type="term" value="C:Myb complex"/>
        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0017053">
        <property type="term" value="C:transcription repressor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR028226">
        <property type="entry name" value="LIN37"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31336">
        <property type="entry name" value="LIN37 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31336:SF3">
        <property type="entry name" value="PROTEIN LIN-37 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15306">
        <property type="entry name" value="LIN37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="160"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="191"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000004369.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="260" mass="29529" checksum="292B0200E38BF8FB" modified="2012-04-18" version="2">MAGYGNENDHDSHDARVDFENILQGILEKANAGDSLSGSQSGNDDDKDSETSETPTRLRMVLPASAGTPPKPSTPSKRKRRKREDYKEEKTKTSSFIIKLFDRSVDLVNFTEDTPLYPICRAWMDNDPTNKINKQTMESQFSSQEDIATSNSEDEEETIITSLPPPNVPFNNIGEDGKYQSPRIPQPEPQPNDSIENYLHKNSVTPPADQLLLDHLQHWRNVRNNWKQKSSENDARYQDGMNLLSDMFNRHAVYNTSVSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>F6WCR1</accession>
    <accession>A0A1W2WD74</accession>
    <name>F6WCR1_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">DNA polymerase</fullName>
            <ecNumber evidence="21">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="26" type="primary">LOC100178968</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26">wild type</strain>
        </source>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20 21">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 21">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 21">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 21">Belongs to the DNA polymerase type-B family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="21"/>
    <dbReference type="EMBL" id="EAAA01001235">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002125320.1">
        <property type="nucleotide sequence ID" value="XM_002125284.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6WCR1"/>
    <dbReference type="FunCoup" id="F6WCR1">
        <property type="interactions" value="700"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000005791"/>
    <dbReference type="Ensembl" id="ENSCINT00000005791.3">
        <property type="protein sequence ID" value="ENSCINP00000005791.3"/>
        <property type="gene ID" value="ENSCING00000002813.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100178968"/>
    <dbReference type="KEGG" id="cin:100178968"/>
    <dbReference type="GeneTree" id="ENSGT00560000077365"/>
    <dbReference type="HOGENOM" id="CLU_000203_2_0_1"/>
    <dbReference type="InParanoid" id="F6WCR1"/>
    <dbReference type="OMA" id="CNNCRPR"/>
    <dbReference type="OrthoDB" id="2414538at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043625">
        <property type="term" value="C:delta DNA polymerase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008296">
        <property type="term" value="F:3'-5'-DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006287">
        <property type="term" value="P:base-excision repair, gap-filling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045004">
        <property type="term" value="P:DNA replication proofreading"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006297">
        <property type="term" value="P:nucleotide-excision repair, DNA gap filling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd05777">
        <property type="entry name" value="DNA_polB_delta_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05533">
        <property type="entry name" value="POLBc_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.132.60:FF:000001">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.690:FF:000001">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.342.10:FF:000003">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000351">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.60">
        <property type="entry name" value="DNA polymerase family B, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.342.10">
        <property type="entry name" value="DNA Polymerase, chain B, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.690">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1600.10">
        <property type="entry name" value="Palm domain of DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006172">
        <property type="entry name" value="DNA-dir_DNA_pol_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017964">
        <property type="entry name" value="DNA-dir_DNA_pol_B_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006133">
        <property type="entry name" value="DNA-dir_DNA_pol_B_exonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006134">
        <property type="entry name" value="DNA-dir_DNA_pol_B_multi_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042087">
        <property type="entry name" value="DNA_pol_B_thumb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023211">
        <property type="entry name" value="DNA_pol_palm_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050240">
        <property type="entry name" value="DNA_pol_type-B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056435">
        <property type="entry name" value="DPOD/Z_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025687">
        <property type="entry name" value="Znf-C4pol"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00592">
        <property type="entry name" value="pol2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10322">
        <property type="entry name" value="DNA POLYMERASE CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10322:SF23">
        <property type="entry name" value="DNA POLYMERASE DELTA CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00136">
        <property type="entry name" value="DNA_pol_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03104">
        <property type="entry name" value="DNA_pol_B_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24055">
        <property type="entry name" value="POL3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14260">
        <property type="entry name" value="zf-C4pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00106">
        <property type="entry name" value="DNAPOLB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00486">
        <property type="entry name" value="POLBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00116">
        <property type="entry name" value="DNA_POLYMERASE_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 21" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="10" id="KW-0228">DNA excision</keyword>
    <keyword evidence="7 21" id="KW-0235">DNA replication</keyword>
    <keyword evidence="18 21" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="15 21" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="14" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="16 21" id="KW-0408">Iron</keyword>
    <keyword evidence="17 21" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9 21" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6 21" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="19 21" id="KW-0539">Nucleus</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 21" id="KW-0808">Transferase</keyword>
    <keyword evidence="13 21" id="KW-0862">Zinc</keyword>
    <keyword evidence="11 21" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DNA polymerase delta/zeta catalytic subunit N-terminal" evidence="25">
        <location>
            <begin position="104"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed DNA polymerase family B exonuclease" evidence="23">
        <location>
            <begin position="201"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed DNA polymerase family B multifunctional" evidence="22">
        <location>
            <begin position="501"/>
            <end position="932"/>
        </location>
    </feature>
    <feature type="domain" description="C4-type zinc-finger of DNA polymerase delta" evidence="24">
        <location>
            <begin position="972"/>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
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        <source>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU000442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF00136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF03104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF14260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="Pfam" id="PF24055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000005791.3"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="1064" mass="120274" checksum="352F0D0E923BC843" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>F6WLH1</accession>
    <name>F6WLH1_CIOIN</name>
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        <recommendedName>
            <fullName evidence="3">1,4-alpha-glucan branching enzyme</fullName>
            <ecNumber evidence="3">2.4.1.18</ecNumber>
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    <organism evidence="7 8">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Tunicata</taxon>
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            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <reaction evidence="1">
            <text>Transfers a segment of a (1-&gt;4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.</text>
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        </reaction>
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        <text evidence="2">Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.</text>
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    <dbReference type="AlphaFoldDB" id="F6WLH1"/>
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    <dbReference type="Ensembl" id="ENSCINT00000022509.2">
        <property type="protein sequence ID" value="ENSCINP00000022263.2"/>
        <property type="gene ID" value="ENSCING00000011709.2"/>
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    <dbReference type="HOGENOM" id="CLU_011131_2_2_1"/>
    <dbReference type="InParanoid" id="F6WLH1"/>
    <dbReference type="OMA" id="FTESHEQ"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
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    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
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        <property type="project" value="GO_Central"/>
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        <property type="term" value="F:1,4-alpha-glucan branching enzyme activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="F:cation binding"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:glycogen biosynthetic process"/>
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        <property type="project" value="GO_Central"/>
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        <property type="entry name" value="E_set_GBE_euk_N"/>
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    </dbReference>
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        <property type="entry name" value="Glucan (1,4-alpha-),-branching enzyme 1b"/>
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        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="A-amylase/branching_C"/>
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        <property type="entry name" value="Branching_enzy"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
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        <property type="entry name" value="GH13_cat_dom"/>
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        <property type="entry name" value="Glyco_hydro_13_N"/>
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    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
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        <property type="entry name" value="Ig-like_fold"/>
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    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
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    <dbReference type="PANTHER" id="PTHR43651">
        <property type="entry name" value="1,4-ALPHA-GLUCAN-BRANCHING ENZYME"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR43651:SF3">
        <property type="entry name" value="1,4-ALPHA-GLUCAN-BRANCHING ENZYME"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02806">
        <property type="entry name" value="Alpha-amylase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02922">
        <property type="entry name" value="CBM_48"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Aamy"/>
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        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="E set domains"/>
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        <property type="entry name" value="Glycosyl hydrolase domain"/>
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    </dbReference>
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    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
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            <begin position="152"/>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009000"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000022263.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="644" mass="75323" checksum="0520693E6620FCA0" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>F6WT98</accession>
    <name>F6WT98_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">HMG box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
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    <comment type="similarity">
        <text evidence="2">Belongs to the TCF/LEF family.</text>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="ENSCING00000002003.3"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
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        <source>
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    <sequence length="351" mass="39394" checksum="C0737B530C961DF5" modified="2012-04-18" version="2">MPQLNSDEAANDEPKTYNDERVGEEDERGWHENDELDNIKGDLVEEEDRDPHRRQSYDVPAKVNSRHRDSSHRSTDDLDRDLPSDIASSRPNFPYLQQDIGAAIALNQQSLLSSLPSSSQVPYNPYAYHHFLYGTPSPTDIDGKPGLPRHNDVPAMYPLPGQLGQIPNPLSPWGPTSPTPFICTASAMAGMLRYPYPFASQFTSTSGGMSAITPFPLVPPHVSGMHSTMIPHPAMALPGHLPNGHNQSKGGVDKKNFGPEPGSKKREKGDKPSRPYVKKPLNAFMLYMKEQRAKVVAECTLKESAAINQILGRKWHSLNREEQQKYYEMARKERQLHQQMFPGWSARDNYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
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    <name>F6WZA5_CIOIN</name>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
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            <taxon>Tunicata</taxon>
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            <taxon>Phlebobranchia</taxon>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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            <authorList>
                <consortium name="Ensembl"/>
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        <property type="entry name" value="Ras guanine-nucleotide exchange factors catalytic domain"/>
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        <property type="entry name" value="PROTEIN SON OF SEVENLESS"/>
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        <name type="scientific">Ciona intestinalis</name>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
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                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="5">wild type</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <source>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
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                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
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                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
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                <person name="Nomoto H."/>
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                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
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                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
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                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
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        </citation>
        <scope>IDENTIFICATION</scope>
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                <consortium name="Ensembl"/>
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    <evidence type="ECO:0000256" key="1">
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            <dbReference type="SAM" id="Phobius"/>
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        <source>
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    <organism evidence="9 10">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">wild type</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5 6">Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the calponin family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002050">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002123605.1">
        <property type="nucleotide sequence ID" value="XM_002123569.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6XVZ5"/>
    <dbReference type="FunCoup" id="F6XVZ5">
        <property type="interactions" value="14"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000015363"/>
    <dbReference type="Ensembl" id="ENSCINT00000015363.3">
        <property type="protein sequence ID" value="ENSCINP00000015363.3"/>
        <property type="gene ID" value="ENSCING00000007496.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100186251"/>
    <dbReference type="KEGG" id="cin:100186251"/>
    <dbReference type="GeneTree" id="ENSGT00940000154539"/>
    <dbReference type="HOGENOM" id="CLU_055232_1_1_1"/>
    <dbReference type="InParanoid" id="F6XVZ5"/>
    <dbReference type="OMA" id="FRCMENI"/>
    <dbReference type="OrthoDB" id="21595at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031032">
        <property type="term" value="P:actomyosin structure organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00014">
        <property type="entry name" value="CH_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050606">
        <property type="entry name" value="Calponin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001997">
        <property type="entry name" value="Calponin/LIMCH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000557">
        <property type="entry name" value="Calponin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003096">
        <property type="entry name" value="SM22_calponin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47385">
        <property type="entry name" value="CALPONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47385:SF25">
        <property type="entry name" value="CALPONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00402">
        <property type="entry name" value="Calponin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00889">
        <property type="entry name" value="CALPONIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00888">
        <property type="entry name" value="SM22CALPONIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01052">
        <property type="entry name" value="CALPONIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51122">
        <property type="entry name" value="CALPONIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3 6" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="8">
        <location>
            <begin position="24"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="178"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="190"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000015363.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="199" mass="22362" checksum="CD79D0A1EC01D2B5" modified="2011-07-27" version="1">MANQGPSYGFSREVRNKMASKYDEYLEEDLREWIAKTSDCQIMSEKTFQSWLKDGRVLCFLIEKLQPDLLKKGMPHETMLNPFRCMENINAFLDAIKLMGVPEPSLCTTADLYDGSGMSQVQTCLLALVDVAISKDLTETDIGIRIPKKQTKEWSESQLKAGDTIIGLQAGTNKYASQSGMTAYGSSRPAYDRKYSSQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>F6YI25</accession>
    <accession>A0A1W2WIA0</accession>
    <name>F6YI25_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">tRNA (cytosine(38)-C(5))-methyltransferase</fullName>
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        </recommendedName>
        <alternativeName>
            <fullName evidence="6">DNA (cytosine-5)-methyltransferase-like protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100183289</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">wild type</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="7 8">Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.204" evidence="4"/>
    <dbReference type="EMBL" id="EAAA01001287">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002128135.1">
        <property type="nucleotide sequence ID" value="XM_002128099.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6YI25"/>
    <dbReference type="SMR" id="F6YI25"/>
    <dbReference type="FunCoup" id="F6YI25">
        <property type="interactions" value="86"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000008432"/>
    <dbReference type="Ensembl" id="ENSCINT00000008432.3">
        <property type="protein sequence ID" value="ENSCINP00000008432.3"/>
        <property type="gene ID" value="ENSCING00000004088.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100183289"/>
    <dbReference type="KEGG" id="cin:100183289"/>
    <dbReference type="GeneTree" id="ENSGT00390000016416"/>
    <dbReference type="HOGENOM" id="CLU_049101_0_0_1"/>
    <dbReference type="InParanoid" id="F6YI25"/>
    <dbReference type="OMA" id="HYAFKYA"/>
    <dbReference type="OrthoDB" id="414133at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.120.10">
        <property type="entry name" value="DNA Methylase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050750">
        <property type="entry name" value="C5-MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001525">
        <property type="entry name" value="C5_MeTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031303">
        <property type="entry name" value="C5_meth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00675">
        <property type="entry name" value="dcm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46098">
        <property type="entry name" value="TRNA (CYTOSINE(38)-C(5))-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46098:SF1">
        <property type="entry name" value="TRNA (CYTOSINE(38)-C(5))-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00145">
        <property type="entry name" value="DNA_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00105">
        <property type="entry name" value="C5METTRFRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00095">
        <property type="entry name" value="C5_MTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51679">
        <property type="entry name" value="SAM_MT_C5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="205"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00039081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00039681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00042810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000008432.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="343" mass="39293" checksum="45FF5C235D5A09A9" modified="2011-07-27" version="1">MEPELKVLELYSGIGGMHYALLGANLKNCEVVCSVDISPAASLVYKHNFPGTKHWERSIEGFSAKDFDNMGFNTLMMSPPCQPFTRVGLQKDINDPRTRSFIYLMKVLPQLSNPPTYILMENVKGFENSKAHDMFLQVLEQLEYSTAQFLLTPKQFGVPNSRLRYYLLAKRKPLQFPDEVNTPNEVIETMPQALQKCLNSNAGKEQNEKHTIKTLENYLEEAESTLKEHALTEKTLLRYLQVMDIVTPRHQSSTCFTKSYGYYAEGTGSVLNMGGEIKMSNPLHNNLRYFTAREVANIMCFPKDKFHFPENFTRKQKYKLLGNSLNVYVVSCLLKLLITDTKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>F6YRI1</accession>
    <name>F6YRI1_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">wild type</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCD family. Peroxisomal fatty acyl CoA transporter (TC 3.A.1.203) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001557">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="EAAA01001558">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6YRI1"/>
    <dbReference type="FunCoup" id="F6YRI1">
        <property type="interactions" value="18"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000014767"/>
    <dbReference type="Ensembl" id="ENSCINT00000014767.3">
        <property type="protein sequence ID" value="ENSCINP00000014767.3"/>
        <property type="gene ID" value="ENSCING00000007200.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182955"/>
    <dbReference type="HOGENOM" id="CLU_007587_1_1_1"/>
    <dbReference type="InParanoid" id="F6YRI1"/>
    <dbReference type="OMA" id="DIQAGHF"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042626">
        <property type="term" value="F:ATPase-coupled transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005324">
        <property type="term" value="F:long-chain fatty acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015910">
        <property type="term" value="P:long-chain fatty acid import into peroxisome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007031">
        <property type="term" value="P:peroxisome organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042760">
        <property type="term" value="P:very long-chain fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03223">
        <property type="entry name" value="ABCD_peroxisomal_ALDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000800">
        <property type="entry name" value="ATP-binding cassette sub-family D member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050835">
        <property type="entry name" value="ABC_transporter_sub-D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11384:SF67">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY D MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11384">
        <property type="entry name" value="ATP-BINDING CASSETTE, SUB-FAMILY D MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06472">
        <property type="entry name" value="ABC_membrane_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-1278">Translocase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transmembrane type-1" evidence="13">
        <location>
            <begin position="100"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="468"/>
            <end position="694"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000014767.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="742" mass="84375" checksum="71B2D73A1875E47F" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>F6YYG7</accession>
    <name>F6YYG7_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glycine cleavage system P protein</fullName>
            <ecNumber evidence="7">1.4.4.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100176441</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">wild type</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">The glycine cleavage system catalyzes the degradation of glycine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 7">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[glycine-cleavage complex H protein] + glycine + H(+) = N(6)-[(R)-S(8)-aminomethyldihydrolipoyl]-L-lysyl-[glycine-cleavage complex H protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:24304"/>
            <dbReference type="Rhea" id="RHEA-COMP:10494"/>
            <dbReference type="Rhea" id="RHEA-COMP:10495"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83143"/>
            <dbReference type="EC" id="1.4.4.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 6 7">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="7">The glycine cleavage system is composed of four proteins: P, T, L and H.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the GcvP family.</text>
    </comment>
    <dbReference type="EC" id="1.4.4.2" evidence="7"/>
    <dbReference type="EMBL" id="EAAA01002202">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="F6YYG7"/>
    <dbReference type="FunCoup" id="F6YYG7">
        <property type="interactions" value="15"/>
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    <dbReference type="STRING" id="7719.ENSCINP00000012269"/>
    <dbReference type="Ensembl" id="ENSCINT00000012269.3">
        <property type="protein sequence ID" value="ENSCINP00000012269.3"/>
        <property type="gene ID" value="ENSCING00000005921.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000017970"/>
    <dbReference type="HOGENOM" id="CLU_004620_3_2_1"/>
    <dbReference type="InParanoid" id="F6YYG7"/>
    <dbReference type="OMA" id="RNLICTC"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 5"/>
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    <dbReference type="GO" id="GO:0005960">
        <property type="term" value="C:glycine cleavage complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016594">
        <property type="term" value="F:glycine binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004375">
        <property type="term" value="F:glycine dehydrogenase (decarboxylating) activity"/>
        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019464">
        <property type="term" value="P:glycine decarboxylation via glycine cleavage system"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00613">
        <property type="entry name" value="GDC-P"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000153">
        <property type="entry name" value="Glycine dehydrogenase (decarboxylating)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000005">
        <property type="entry name" value="Glycine dehydrogenase (decarboxylating), mitochondrial"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="3.40.640.10:FF:000007">
        <property type="entry name" value="glycine dehydrogenase (Decarboxylating), mitochondrial"/>
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    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00711">
        <property type="entry name" value="GcvP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003437">
        <property type="entry name" value="GcvP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049316">
        <property type="entry name" value="GDC-P_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049315">
        <property type="entry name" value="GDC-P_N"/>
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    <dbReference type="InterPro" id="IPR020581">
        <property type="entry name" value="GDC_P"/>
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    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
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    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00461">
        <property type="entry name" value="gcvP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003346">
        <property type="entry name" value="PRK04366.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11773:SF1">
        <property type="entry name" value="GLYCINE DEHYDROGENASE (DECARBOXYLATING), MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11773">
        <property type="entry name" value="GLYCINE DEHYDROGENASE, DECARBOXYLATING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21478">
        <property type="entry name" value="GcvP2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02347">
        <property type="entry name" value="GDC-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3 6 7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Glycine cleavage system P-protein N-terminal" evidence="8">
        <location>
            <begin position="47"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="domain" description="Glycine dehydrogenase C-terminal" evidence="9">
        <location>
            <begin position="815"/>
            <end position="936"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="6">
        <location>
            <position position="738"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049026"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR603437-50"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364056"/>
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    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02347"/>
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    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF21478"/>
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    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000012269.3"/>
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    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>F6Z4I8</accession>
    <accession>A0A1W2W1A4</accession>
    <name>F6Z4I8_CIOIN</name>
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        <submittedName>
            <fullName evidence="8">Leukocyte cell-derived chemotaxin 1</fullName>
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    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100186942</name>
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    <organism evidence="8 9">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Phlebobranchia</taxon>
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            <taxon>Ciona</taxon>
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    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">wild type</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="EAAA01001097">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="EAAA01001098">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002120724.1">
        <property type="nucleotide sequence ID" value="XM_002120688.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6Z4I8"/>
    <dbReference type="STRING" id="7719.ENSCINP00000006990"/>
    <dbReference type="Ensembl" id="ENSCINT00000006990.3">
        <property type="protein sequence ID" value="ENSCINP00000006990.3"/>
        <property type="gene ID" value="ENSCING00000016942.2"/>
    </dbReference>
    <dbReference type="GeneID" id="100186942"/>
    <dbReference type="KEGG" id="cin:100186942"/>
    <dbReference type="GeneTree" id="ENSGT00480000042679"/>
    <dbReference type="HOGENOM" id="CLU_071852_0_0_1"/>
    <dbReference type="InParanoid" id="F6Z4I8"/>
    <dbReference type="OMA" id="GNLPIFW"/>
    <dbReference type="OrthoDB" id="5985282at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043405">
        <property type="entry name" value="Chondromodulin/Tenomodulin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14064">
        <property type="entry name" value="CHONDROMODULIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14064:SF5">
        <property type="entry name" value="LEUKOCYTE CELL-DERIVED CHEMOTAXIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="39"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="225"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="230"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="258"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000006990.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="350" mass="39697" checksum="9C01D1851BD503B6" modified="2012-04-18" version="2">MTAGNKIDLLVKDDGSTEALVATKPVLLKRRQPTLAQRFSVVTLTCLIALLVCGAITAYMVYRGHMRHLFVPQHKSGMWCGTTYQMNGNLERGELEFNTTDQSEKFITKNTEATMDGLEGKTGIKLIAPDKKEICLVRDDFKYGKAGPPLLPRKELLEKFMEQDRELDVVMDDKIWIADGKINSDFEIPPSIQFTCNGKPIFGMRPMTDADFMIPEMDDDDLMGTLSIGDDYKDDHPDYDVEKRGISEDNEYTPTEEPPSDENDPSDENEPSEENEPEAPGQDGSYIGQTQTDYDLPCCNHCTRPHLQCSQVCVPLQTPNYPYFYRNCRYQQCVVYMPCSWWHARVLGAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>F6Z8E9</accession>
    <accession>A0A1W3JJS7</accession>
    <name>F6Z8E9_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Heparan-sulfate 6-O-sulfotransferase</fullName>
            <ecNumber evidence="8">2.8.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100182982</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">wild type</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">6-O-sulfation enzyme which catalyzes the transfer of sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to position 6 of the N-sulfoglucosamine residue (GlcNS) of heparan sulfate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>alpha-D-glucosaminyl-[heparan sulfate](n) + 3'-phosphoadenylyl sulfate = 6-sulfo-alpha-D-glucosaminyl-[heparan sulfate](n) + adenosine 3',5'-bisphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:56604"/>
            <dbReference type="Rhea" id="RHEA-COMP:9830"/>
            <dbReference type="Rhea" id="RHEA-COMP:14621"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:58388"/>
            <dbReference type="ChEBI" id="CHEBI:140604"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Membrane</location>
            <topology evidence="8">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the sulfotransferase 6 family.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="8"/>
    <dbReference type="EMBL" id="EAAA01000278">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009862399.1">
        <property type="nucleotide sequence ID" value="XM_009864097.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6Z8E9"/>
    <dbReference type="FunCoup" id="F6Z8E9">
        <property type="interactions" value="102"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000016267"/>
    <dbReference type="Ensembl" id="ENSCINT00000016267.3">
        <property type="protein sequence ID" value="ENSCINP00000016267.3"/>
        <property type="gene ID" value="ENSCING00000007953.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100182982"/>
    <dbReference type="KEGG" id="cin:100182982"/>
    <dbReference type="GeneTree" id="ENSGT00950000183071"/>
    <dbReference type="HOGENOM" id="CLU_027877_0_0_1"/>
    <dbReference type="InParanoid" id="F6Z8E9"/>
    <dbReference type="OMA" id="IYAYQEG"/>
    <dbReference type="OrthoDB" id="406981at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017095">
        <property type="term" value="F:heparan sulfate 6-sulfotransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015012">
        <property type="term" value="P:heparan sulfate proteoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010635">
        <property type="entry name" value="Heparan_SO4-6-sulfoTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005331">
        <property type="entry name" value="Sulfotransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12812">
        <property type="entry name" value="HEPARAN SULFATE 6-O-SULFOTRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12812:SF0">
        <property type="entry name" value="HEPARAN-SULFATE 6-O-SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03567">
        <property type="entry name" value="Sulfotransfer_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="17"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000016267.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="382" mass="44158" checksum="2E634F2D15F9D55F" modified="2011-07-27" version="1">MNNVLQKFSLKEIQKKAFIWIVLASLIFLFYLSLQATNTGFSEKPLTNKASSTKKSNTVSSANNVKQLGVVIPSTTLNNIGQHDLSATKVLPMATIQTNMSIAVDFDINSTDVMVVLHMQKTSGSTFGRHLVRDLEPPFTCEYVQKKMSLCPRPNGQETWLFSRFSRGWRCGVHAGWTSLTECVPLLFHDPKLERRYFYITVLREPVARFLSEWGHVRRAATWDQHKFLCNKVQTPQKVCWGEALDWRNASLEEFINCDINFGRNRQTRMLADLKLVNCSAFGDEEHDRIMLKSAKHNLMSMPFFGLAERQVDSQALFEQTFGIKFTKQFVDKSETHVSNIVLDEEVSDKIRKLNHLDVQLYKYAKEIFARRYERIRKDKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>F6ZFM9</accession>
    <name>F6ZFM9_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="8">Belongs to the protein kinase superfamily.</text>
    </comment>
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    <dbReference type="AlphaFoldDB" id="F6ZFM9"/>
    <dbReference type="FunCoup" id="F6ZFM9">
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    <dbReference type="STRING" id="7719.ENSCINP00000012550"/>
    <dbReference type="Ensembl" id="ENSCINT00000012550.3">
        <property type="protein sequence ID" value="ENSCINP00000012550.3"/>
        <property type="gene ID" value="ENSCING00000006077.3"/>
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    <dbReference type="GeneTree" id="ENSGT00940000169503"/>
    <dbReference type="HOGENOM" id="CLU_000288_63_5_1"/>
    <dbReference type="InParanoid" id="F6ZFM9"/>
    <dbReference type="OMA" id="MENEYHA"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000169">
        <property type="entry name" value="Serine/threonine-protein kinase 32A"/>
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    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Kinase-like_dom_sf"/>
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        <property type="entry name" value="Prot_kinase_dom"/>
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    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
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    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
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        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR KINASE/RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24355:SF30">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE 32B ISOFORM X1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PROTEIN_KINASE_ST"/>
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    </dbReference>
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    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
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        <location>
            <begin position="24"/>
            <end position="285"/>
        </location>
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        <location>
            <begin position="327"/>
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        </location>
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        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000012550.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="346" mass="40345" checksum="029867CCFC796F12" modified="2012-04-18" version="1">MGTKSSKNEDGDDDPSADVGFNDFEVLRSIGKGSFGKVCIVRRKKTENEVYAMKYMNKLSSWKHGAIDHVLRELEILSTLRHQFLVNLWFTFQDEEDIFMVSDLLMGGDLRYHIQRHVHFTFDDVRIYFCELALVLDYLRNKHIIHRDIKPDNILLDERGHLHLTDFNIATVLNDEHPCAKSFAGTKPYYDITAPEIFNSSSGYDYAVDWWSLGVTMYEVLRHKRPFNIGSDTTNEEVTALHNDGSISFPVDWGQPIMNLFFKLFKVDPTKRIQSFESLISEEFCQNMEADDVMRLKGQPTFQPSQKELNYDPTFELEEMIIESNPLHKKKQRIQKLKKNSQKKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
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    <name>F6ZMR7_CIOIN</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Phlebobranchia</taxon>
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        </lineage>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="protein sequence ID" value="ENSCINP00000022191.2"/>
        <property type="gene ID" value="ENSCING00000011657.2"/>
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    <dbReference type="InParanoid" id="F6ZMR7"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000022191.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="281" mass="30435" checksum="B3C2E7DA36A0937D" modified="2012-04-18" version="2">MVSEAWDQKIQHQHMHSQSAEADIMDIQLQSLLSSSSTSGITAATNITPHSIPSYGHASHQCRTVVNQDNSSFSNSAEINVVHQTNNQNAVTHKQHSLPANFAQSSNHQTSTKMFYPQSLMQQGSGNHFMSDPGQFNRALVTPELGNSDPSNNLRFHTTDSYGSMIHSSQHIPIITSTSGNWSQGTVVSATASSYKVPTTNTAEQHMLQSPGPNHSSFPLTISNQSSSKIIPQNISVEEMKQEKGQNHQGMSMASPLLVTLLQTEACTTGLSAIQTLAHTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>F6ZTJ9</accession>
    <name>F6ZTJ9_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="F6ZTJ9"/>
    <dbReference type="FunCoup" id="F6ZTJ9">
        <property type="interactions" value="780"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000019832"/>
    <dbReference type="Ensembl" id="ENSCINT00000019832.3">
        <property type="protein sequence ID" value="ENSCINP00000019832.3"/>
        <property type="gene ID" value="ENSCING00000009762.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00900000141107"/>
    <dbReference type="HOGENOM" id="CLU_007172_0_0_1"/>
    <dbReference type="InParanoid" id="F6ZTJ9"/>
    <dbReference type="OMA" id="LWARYIL"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12391">
        <property type="entry name" value="RRM1_SART3"/>
        <property type="match status" value="1"/>
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                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. Required for the assembly of the dynein-dynactin and MAD1-MAD2 complexes onto kinetochores. Its function related to the spindle assembly machinery is proposed to depend on its association in the mitotic RZZ complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the RZZ complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Chromosome</location>
            <location evidence="1 9">Centromere</location>
            <location evidence="1 9">Kinetochore</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the ZWILCH family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="F7AIJ0"/>
    <dbReference type="FunCoup" id="F7AIJ0">
        <property type="interactions" value="308"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000029346"/>
    <dbReference type="Ensembl" id="ENSCINT00000029592.2">
        <property type="protein sequence ID" value="ENSCINP00000029346.2"/>
        <property type="gene ID" value="ENSCING00000017264.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000013696"/>
    <dbReference type="HOGENOM" id="CLU_800610_0_0_1"/>
    <dbReference type="InParanoid" id="F7AIJ0"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990423">
        <property type="term" value="C:RZZ complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007094">
        <property type="term" value="P:mitotic spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034501">
        <property type="term" value="P:protein localization to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018630">
        <property type="entry name" value="Zwilch"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15995">
        <property type="entry name" value="PROTEIN ZWILCH HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15995:SF1">
        <property type="entry name" value="PROTEIN ZWILCH HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09817">
        <property type="entry name" value="Zwilch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4 9" id="KW-0132">Cell division</keyword>
    <keyword evidence="8 9" id="KW-0137">Centromere</keyword>
    <keyword evidence="3 9" id="KW-0158">Chromosome</keyword>
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    <keyword evidence="5 9" id="KW-0498">Mitosis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU369076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000029346.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="347" mass="39395" checksum="8921EEB5B20C5AE6" modified="2012-04-18" version="2">SSLAMESFSRFELFSESDRIIKAIEENASHVSIDVSADEVVNILETPHQKIHTVAVNLRPVPGDPRSLVYFLHQDIKILEDLFASGLMEALSPKQEVVWADITDEESVEISIVDKIDKVLKSFYKPTKNEAKTDDKTNDDELISSLFAPRQEFDFTECVWEVLKIVQSYQELIKCLGLIMKGLEDGKGVCFWLHENNRSYLANIIRQSYQGKITAPKFGSTLPIQVLIEIGIEKLGQDFAQFFLSHRFATREKLEKYMSPDDVIDDDVIRLQNKLVGLKKLHHITELASHCSSNLMLPMEKINKVVRSALSSYTTEDFDSSKLFSFNVSLGDVKGLLECSTPSLWRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>F7AK67</accession>
    <name>F7AK67_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">wild type</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01000814">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="EAAA01000815">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="EAAA01000816">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="EAAA01000817">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="EAAA01000818">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F7AK67"/>
    <dbReference type="Ensembl" id="ENSCINT00000029270.2">
        <property type="protein sequence ID" value="ENSCINP00000029024.2"/>
        <property type="gene ID" value="ENSCING00000025087.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000169134"/>
    <dbReference type="HOGENOM" id="CLU_2008997_0_0_1"/>
    <dbReference type="InParanoid" id="F7AK67"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024731">
        <property type="entry name" value="NELL2-like_EGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24038:SF11">
        <property type="entry name" value="INTEGRIN BETA-LIKE PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24038">
        <property type="entry name" value="STABILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12947">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="2 6" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EGF-like" evidence="7">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="7">
        <location>
            <begin position="42"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="46"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000029024.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="124" mass="13481" checksum="15B681F7A0FEDC1E" modified="2012-04-18" version="2">CHPTLATCIYNPTTRQYRCQCIAGYTGNGVTCSLPTNAAPIVVPRCRNVCHRDADCVQVNGQGVCRCKQNFRGDGVSLCSRISNQCLNCHSHATCRQIQRVFSCVCNENYIGDGVNYCTAVIAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>F7AQR7</accession>
    <accession>A0A1W2WAV9</accession>
    <name>F7AQR7_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cell wall protein PRY3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100176841</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
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    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="molecule type" value="Genomic_DNA"/>
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<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>F7ATQ3</accession>
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    <reference evidence="17" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Syntaxin that may be involved in targeting and fusion of Golgi-derived retrograde transport vesicles with the ER.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Endomembrane system</location>
            <topology evidence="13">Single-pass type IV membrane protein</topology>
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            <topology evidence="2">Single-pass membrane protein</topology>
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    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the syntaxin family.</text>
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        <property type="gene ID" value="ENSCING00000004501.3"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="GO_Central"/>
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    <keyword evidence="12 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="10 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
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            <end position="290"/>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00046280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF10496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000009317.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="291" mass="33659" checksum="BD0B3457899C6B1C" modified="2011-07-27" version="1">MIDRTPEFLKLLKQIRKNEGIPAKSILTSQKKILKSSFEIKSRGIVTNLTQLRDFLLDNRHGYLTRDETLMTNQEKDKIDADGEKFMQICSRSLEQLKQDTPTNLTTQVGFHQKLVVEIVEKYLKLVFQIYNEQKSVRVKQIVERNTVSQLRLNSTRAKSSERLPGQVKHNPKYLFEESDDDGLVPDDDETFAQENAQLLSDMNSLSDEVRSIGGKVVEIAKLHEMFSEQVFQQEADIELVSEKIVESSENVKDGNEELREAIKNNAGFRVWVLFFLVMCSFSLLFLEWYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>F7AYD9</accession>
    <accession>F7AYD1</accession>
    <name>F7AYD9_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Cytosolic Fe-S cluster assembly factor NUBP2 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100177321</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. The NUBP1-NUBP2 heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins. Negatively regulates cilium formation and structure.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Heterotetramer of 2 NUBP1 and 2 NUBP2 chains. Interacts with KIFC1. Interacts with NUBP1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Cilium axoneme</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
            <location evidence="1">Centriole</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="F7AYD9"/>
    <dbReference type="FunCoup" id="F7AYD9">
        <property type="interactions" value="87"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000007293"/>
    <dbReference type="Ensembl" id="ENSCINT00000007293.3">
        <property type="protein sequence ID" value="ENSCINP00000007293.3"/>
        <property type="gene ID" value="ENSCING00000016721.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183193"/>
    <dbReference type="HOGENOM" id="CLU_024839_0_1_1"/>
    <dbReference type="InParanoid" id="F7AYD9"/>
    <dbReference type="OMA" id="DCTNVWG"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140663">
        <property type="term" value="F:ATP-dependent FeS chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02037">
        <property type="entry name" value="Mrp_NBP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000796">
        <property type="entry name" value="Cytosolic Fe-S cluster assembly factor NUBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000808">
        <property type="entry name" value="Mrp-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019591">
        <property type="entry name" value="Mrp/NBP35_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033756">
        <property type="entry name" value="YlxH/NBP35"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23264:SF19">
        <property type="entry name" value="CYTOSOLIC FE-S CLUSTER ASSEMBLY FACTOR NUBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23264">
        <property type="entry name" value="NUCLEOTIDE-BINDING PROTEIN NBP35 YEAST -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10609">
        <property type="entry name" value="ParA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01215">
        <property type="entry name" value="MRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00053368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00065349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000007293.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="220" mass="24089" checksum="39E6F49628F34DD2" modified="2013-01-09" version="3">KKVGILDTDICGPSIPRMLNLENASVFQCDQGWVPVFATEDEKLCVMSIAFMLNGKDDPVIWRGPKKTAMIKQFITDVHWGDLDYLIIDTPPGTSDEHLSVVQNSKGKVKGAILVTTPQTVAVSDVRRELTFCRKTSIPIIGVVENMCGFVCPHCSECSLVFSQGGGEALAKQEGLDFLARIPLDPDLAKCCEDGKKMINVFPDSKTLSSINQLVDKMLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>F7AZQ1</accession>
    <name>F7AZQ1_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC494385</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="F7AZQ1"/>
    <dbReference type="STRING" id="7719.ENSCINP00000002055"/>
    <dbReference type="Ensembl" id="ENSCINT00000002055.3">
        <property type="protein sequence ID" value="ENSCINP00000002055.3"/>
        <property type="gene ID" value="ENSCING00000001094.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01130000278323"/>
    <dbReference type="HOGENOM" id="CLU_562094_0_0_1"/>
    <dbReference type="InParanoid" id="F7AZQ1"/>
    <dbReference type="OMA" id="AMFVIIM"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
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        <property type="entry name" value="GPCR_Rhodpsn"/>
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        <property type="entry name" value="NEUROPEPTIDES RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SOMATOSTATIN RECEPTOR TYPE 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="GPCRRHODOPSN"/>
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        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="116"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="155"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="257"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="332"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="370"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="55"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50262"/>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000002055.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="495" mass="56594" checksum="6321FEBC387F3428" modified="2011-07-27" version="1">MNEPISYEYYQPSHENFSEYNLGSLPQNDECSAVPAGPKWLPALQISIALCGLIFNAIAMFVIIMLQDFKKSISHWYVLQLAIADSLFLAMLPFKASESLSGSWNLPHFLCHLQQAVFMLNYYAGIFFLTVMSFDRYAAIVHPVSAPWRRLRTHGNAVLITLAVWALAIAVSIPLFVWSDVKHCKCTYMFPRSAEEFQQHHGVNNSTDYVDYEEFMNVMNEMSQETTVELQKTAEANPMACANEPWVSMKIWAGMHFVFAFLLPFIVMTVCYSLITWRVFHPAVSSANRRAGRRKSSCATPRARLFSSFQFSTRSNSSGSKKYPGITQKSRVTIIVVSLVLMFLLCWLPYNIVLLAKFAELPYSGETCTLIENVTIVLVYLNSMINPVLYTFLGSRFFRRFSKARLVFCRNRQFGGSVNSSTNRSNTFRQRLGEIRAWKTTSARFSETSTTQQPPCSSRIYQPHDNKEEVFVMHRTRSNDEHEPEIIVETKLLSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>F7B7E9</accession>
    <name>F7B7E9_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Methionine--tRNA ligase, mitochondrial</fullName>
            <ecNumber evidence="2">6.1.1.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Methionyl-tRNA synthetase 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Mitochondrial methionyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">wild type</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>tRNA(Met) + L-methionine + ATP = L-methionyl-tRNA(Met) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:13481"/>
            <dbReference type="Rhea" id="RHEA-COMP:9667"/>
            <dbReference type="Rhea" id="RHEA-COMP:9698"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78530"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.10"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.10" evidence="2"/>
    <dbReference type="EMBL" id="EAAA01001429">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F7B7E9"/>
    <dbReference type="FunCoup" id="F7B7E9">
        <property type="interactions" value="340"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000010675"/>
    <dbReference type="Ensembl" id="ENSCINT00000010675.3">
        <property type="protein sequence ID" value="ENSCINP00000010675.3"/>
        <property type="gene ID" value="ENSCING00000005183.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00550000075136"/>
    <dbReference type="HOGENOM" id="CLU_009710_9_0_1"/>
    <dbReference type="InParanoid" id="F7B7E9"/>
    <dbReference type="OMA" id="NMFLPDR"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004825">
        <property type="term" value="F:methionine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006431">
        <property type="term" value="P:methionyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00814">
        <property type="entry name" value="MetRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.220.10:FF:000001">
        <property type="entry name" value="methionine--tRNA ligase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.730.10:FF:000022">
        <property type="entry name" value="Methionyl-tRNA synthetase 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.220.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014758">
        <property type="entry name" value="Met-tRNA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023457">
        <property type="entry name" value="Met-tRNA_synth_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015413">
        <property type="entry name" value="Methionyl/Leucyl_tRNA_Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033911">
        <property type="entry name" value="MetRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00398">
        <property type="entry name" value="metG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43326:SF1">
        <property type="entry name" value="METHIONINE--TRNA LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43326">
        <property type="entry name" value="METHIONYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09334">
        <property type="entry name" value="tRNA-synt_1g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01041">
        <property type="entry name" value="TRNASYNTHMET"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 14" id="KW-0436">Ligase</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 14" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Methionyl/Leucyl tRNA synthetase" evidence="15">
        <location>
            <begin position="32"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00026124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF09334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000010675.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="551" mass="63850" checksum="5C3DA7B50E053AF3" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>F7BGK8</accession>
    <name>F7BGK8_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">wild type</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="ENSCINP00000026666.2"/>
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    <dbReference type="InParanoid" id="F7BGK8"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Peptidase_S1_PA"/>
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        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
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        <property type="entry name" value="ACROSIN-RELATED"/>
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        <property type="entry name" value="HYALIN"/>
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        <location>
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    <feature type="domain" description="Peptidase S1" evidence="3">
        <location>
            <begin position="110"/>
            <end position="190"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50240"/>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000026666.2"/>
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        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    <sequence length="190" mass="20335" checksum="B983BA674D7EC3D0" modified="2012-04-18" version="2">MLSTAWIFTGWTVVGTSVTLFGFLFFYDTKTPGNITSTTTTAAPETSTNLIVTTLPTILNTTVFESTSSISTTSNTSIFSCGRPPIVGRSAEALEPVLGPFLVAILKGRIIGGSTALEGQWPWMSYIYERANGIKDGGVCGGTLVNERWVVTAAHCFGTISPDLYSVIFGRHNTSVVSGNEIQRSIIQII</sequence>
</entry>
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    <accession>F7BIC8</accession>
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            <fullName evidence="6">Protein lifeguard 1</fullName>
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    <organism evidence="6 7">
        <name type="scientific">Ciona intestinalis</name>
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        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ciona</taxon>
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    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
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                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
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            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">wild type</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <topology evidence="1">Multi-pass membrane protein</topology>
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    <comment type="similarity">
        <text evidence="5">Belongs to the BI1 family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="FunCoup" id="F7BIC8">
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    <dbReference type="Ensembl" id="ENSCINT00000014392.3">
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000014392.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="311" mass="34833" checksum="020A5817002A98C8" modified="2012-04-18" version="2">MKLWMRRSCLSQTPQPTTYVQASAPLQAGAPYPPANQTYPPANQPYAQPYPTGNYQTQYAPHPAETAVHQPSYNSTPHHAEDGGFTTRGSFDDKSVRRLFIRKVYITLGLQLLVTFGIVCIFTFIPSVKTFIRANSAMYYVAYAIFLALYIALVCCPNVRRKHPTNIIVLAIFTLALSYMVGTIASFYDTMSVVIALGITVGVCVAVTLFSLQTKFDFTKCSGLLFVLVLVLMLFGFVTIFTWNKSWYLHVVYGSLGALVFTLFLAFDTQLIMGGKRYELDPEEYIYGALNLYIDVVYIFIFILSIFGSSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>F7BL09</accession>
    <name>F7BL09_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">wild type</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01002021">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F7BL09"/>
    <dbReference type="STRING" id="7719.ENSCINP00000024710"/>
    <dbReference type="Ensembl" id="ENSCINT00000024956.2">
        <property type="protein sequence ID" value="ENSCINP00000024710.2"/>
        <property type="gene ID" value="ENSCING00000013468.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015400"/>
    <dbReference type="HOGENOM" id="CLU_1911315_0_0_1"/>
    <dbReference type="InParanoid" id="F7BL09"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040008">
        <property type="entry name" value="Ribosomal_mL46"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13124">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L46, MITOCHONDRIAL PRECURSOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13124:SF12">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000024710.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="133" mass="15359" checksum="5C78FA2E8E76E3F1" modified="2012-04-18" version="2">MDFKVANQDIEEMNELEAQQFYEENETITIEDSDRTNINRQPNETLVMVTQQKLGKDNVWMLPVEPWSKEETLRECAERALISHCGTDVGAAFISNGPSGFYKYKFPKDARENSLVGAKLFIYNAYLPRVFIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>H2XJV5</accession>
    <accession>A0A1W2WFG6</accession>
    <name>H2XJV5_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Claudin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100183075</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell junction</location>
            <location evidence="8">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the claudin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002129997.1">
        <property type="nucleotide sequence ID" value="XM_002129961.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XJV5"/>
    <dbReference type="STRING" id="7719.ENSCINP00000029937"/>
    <dbReference type="Ensembl" id="ENSCINT00000030414.1">
        <property type="protein sequence ID" value="ENSCINP00000029937.1"/>
        <property type="gene ID" value="ENSCING00000021125.1"/>
    </dbReference>
    <dbReference type="GeneID" id="100183075"/>
    <dbReference type="KEGG" id="cin:100183075"/>
    <dbReference type="GeneTree" id="ENSGT00940000158624"/>
    <dbReference type="HOGENOM" id="CLU_076370_2_2_1"/>
    <dbReference type="InParanoid" id="H2XJV5"/>
    <dbReference type="OMA" id="QKREFGN"/>
    <dbReference type="OrthoDB" id="10025519at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070830">
        <property type="term" value="P:bicellular tight junction assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006187">
        <property type="entry name" value="Claudin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017974">
        <property type="entry name" value="Claudin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004031">
        <property type="entry name" value="PMP22/EMP/MP20/Claudin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12002">
        <property type="entry name" value="CLAUDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00822">
        <property type="entry name" value="PMP22_Claudin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01077">
        <property type="entry name" value="CLAUDIN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01346">
        <property type="entry name" value="CLAUDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0965">Cell junction</keyword>
    <keyword evidence="3 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0796">Tight junction</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="118"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="161"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU060637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000029937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="215" mass="23015" checksum="0E7ADCA56E01CCE2" modified="2012-04-18" version="1">MVNGGLQIGGFLLGLIGWILGIVTCALPDWRKNDLQGEVIESVIRTTGLWVRCTTQATGLWTCDDYDSFFLGLPVALQAARACVCLSIAMGFLSCICSVFGLECTNISAGNPKMKARITLVAGLLNFIGGVSVGIAVSWFAANVLQEYWNPLGAATSNTRYVYGSALFVGWISMAVSVIAGLVQCCSSWNVDDEDNGRPYTYNPSKPKASSAEYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>H2XJX5</accession>
    <accession>A0A1W2WNI2</accession>
    <name>H2XJX5_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">rhomboid protease</fullName>
            <ecNumber evidence="4">3.4.21.105</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100180919</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">wild type</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Cleaves type-1 transmembrane domains using a catalytic dyad composed of serine and histidine that are contributed by different transmembrane domains.</text>
            <dbReference type="EC" id="3.4.21.105"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the peptidase S54 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.105" evidence="4"/>
    <dbReference type="EMBL" id="EAAA01000673">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002130870.1">
        <property type="nucleotide sequence ID" value="XM_002130834.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XJX5"/>
    <dbReference type="STRING" id="7719.ENSCINP00000029957"/>
    <dbReference type="MEROPS" id="S54.002"/>
    <dbReference type="Ensembl" id="ENSCINT00000032474.1">
        <property type="protein sequence ID" value="ENSCINP00000029957.1"/>
        <property type="gene ID" value="ENSCING00000022105.1"/>
    </dbReference>
    <dbReference type="GeneID" id="100180919"/>
    <dbReference type="KEGG" id="cin:100180919"/>
    <dbReference type="GeneTree" id="ENSGT00940000166075"/>
    <dbReference type="HOGENOM" id="CLU_048023_2_1_1"/>
    <dbReference type="InParanoid" id="H2XJX5"/>
    <dbReference type="OMA" id="KYEHRKH"/>
    <dbReference type="OrthoDB" id="418595at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1540.10:FF:000002">
        <property type="entry name" value="Rhomboid, veinlet-like 3 (Drosophila)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1540.10">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022764">
        <property type="entry name" value="Peptidase_S54_rhomboid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017213">
        <property type="entry name" value="Peptidase_S54_rhomboid_met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051739">
        <property type="entry name" value="Rhomboid_IM_Serine_Proteases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45840:SF2">
        <property type="entry name" value="PROTEIN RHOMBOID-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45840">
        <property type="entry name" value="RHOMBOID-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01694">
        <property type="entry name" value="Rhomboid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037470">
        <property type="entry name" value="Rhomboid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="129"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="177"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="214"/>
            <end position="231"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="237"/>
            <end position="256"/>
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    <feature type="transmembrane region" description="Helical" evidence="11">
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            <begin position="268"/>
            <end position="287"/>
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    <feature type="transmembrane region" description="Helical" evidence="11">
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            <begin position="299"/>
            <end position="319"/>
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    <feature type="transmembrane region" description="Helical" evidence="11">
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            <begin position="331"/>
            <end position="352"/>
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    </feature>
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        <location>
            <begin position="47"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="10">
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            <position position="241"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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    </evidence>
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    </evidence>
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            <dbReference type="Ensembl" id="ENSCINP00000029957.1"/>
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</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>H2XK47</accession>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
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                <person name="Richardson P."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
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            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">wild type</strain>
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    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="1" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="ENSCINP00000030029.1"/>
        <property type="gene ID" value="ENSCING00000024713.1"/>
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        <property type="component" value="Chromosome 10"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="73" mass="8078" checksum="B951F899729E4EB5" modified="2012-04-18" version="1">KGVLSSSPYYIKLLDGVTAYLLNDLNLAKDDQQKNDKKGHLVVPGILKKQASLPSVEPSGQELYRILLQKLVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>H2XK97</accession>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
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    <organism evidence="1 2">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">wild type</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01001227">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="EAAA01001228">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XK97"/>
    <dbReference type="Ensembl" id="ENSCINT00000031596.1">
        <property type="protein sequence ID" value="ENSCINP00000030079.1"/>
        <property type="gene ID" value="ENSCING00000018324.1"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3419357_0_0_1"/>
    <dbReference type="InParanoid" id="H2XK97"/>
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        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000030079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="25" mass="2829" checksum="135D804CD0E750EB" modified="2012-04-18" version="1">MTHSVRCSLPTAQTCSNHYYVDVCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>H2XL55</accession>
    <name>H2XL55_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Protein DIS3 homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Ribosomal RNA-processing protein 44</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC100187471</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">wild type</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 15">Belongs to the RNR ribonuclease family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01000064">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XL55"/>
    <dbReference type="FunCoup" id="H2XL55">
        <property type="interactions" value="1080"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000030387"/>
    <dbReference type="Ensembl" id="ENSCINT00000031719.1">
        <property type="protein sequence ID" value="ENSCINP00000030387.1"/>
        <property type="gene ID" value="ENSCING00000024053.1"/>
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    <dbReference type="GeneTree" id="ENSGT00530000063106"/>
    <dbReference type="HOGENOM" id="CLU_002333_5_0_1"/>
    <dbReference type="InParanoid" id="H2XL55"/>
    <dbReference type="OMA" id="GQVMRNN"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019899">
        <property type="term" value="F:enzyme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:nuclear mRNA surveillance of mRNA 3'-end processing"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="DIS3-like exonuclease 1 isoform X1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Exosome complex exonuclease exoribonuclease (Rrp44)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000125">
        <property type="entry name" value="exosome complex exonuclease RRP44 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.690:FF:000010">
        <property type="entry name" value="Rrp44p homologue, putative"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Dis3_CSD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001900">
        <property type="entry name" value="RNase_II/R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022966">
        <property type="entry name" value="RNase_II/R_CS"/>
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    <dbReference type="InterPro" id="IPR050180">
        <property type="entry name" value="RNR_Ribonuclease"/>
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        <property type="entry name" value="Rrp44_CSD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033770">
        <property type="entry name" value="RRP44_S1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23355:SF35">
        <property type="entry name" value="EXOSOME COMPLEX EXONUCLEASE RRP44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23355">
        <property type="entry name" value="RIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17849">
        <property type="entry name" value="OB_Dis3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13638">
        <property type="entry name" value="PIN_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00773">
        <property type="entry name" value="RNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17216">
        <property type="entry name" value="Rrp44_CSD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17215">
        <property type="entry name" value="Rrp44_S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00670">
        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00955">
        <property type="entry name" value="RNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01175">
        <property type="entry name" value="RIBONUCLEASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="9" id="KW-0271">Exosome</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0540">Nuclease</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="PIN" evidence="16">
        <location>
            <begin position="53"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="RNB" evidence="17">
        <location>
            <begin position="439"/>
            <end position="772"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00077221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00077930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000030387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="916" mass="103266" checksum="5A4DF6DF63605AD9" modified="2012-04-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>H2XNL6</accession>
    <name>H2XNL6_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Gamma-tubulin complex component</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">wild type</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
            <location evidence="5">Cytoskeleton</location>
            <location evidence="5">Microtubule organizing center</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the TUBGCP family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002014">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XNL6"/>
    <dbReference type="FunCoup" id="H2XNL6">
        <property type="interactions" value="32"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000031249"/>
    <dbReference type="Ensembl" id="ENSCINT00000030587.1">
        <property type="protein sequence ID" value="ENSCINP00000031249.1"/>
        <property type="gene ID" value="ENSCING00000020504.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155962"/>
    <dbReference type="HOGENOM" id="CLU_011574_0_0_1"/>
    <dbReference type="InParanoid" id="H2XNL6"/>
    <dbReference type="OMA" id="ILRFWLM"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000930">
        <property type="term" value="C:gamma-tubulin complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043015">
        <property type="term" value="F:gamma-tubulin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007020">
        <property type="term" value="P:microtubule nucleation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051225">
        <property type="term" value="P:spindle assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd22572">
        <property type="entry name" value="GCP5_NTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1900">
        <property type="entry name" value="Gamma-tubulin complex, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007259">
        <property type="entry name" value="GCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059169">
        <property type="entry name" value="GCP5_N_ext"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040457">
        <property type="entry name" value="GCP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042241">
        <property type="entry name" value="GCP_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041470">
        <property type="entry name" value="GCP_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19302">
        <property type="entry name" value="GAMMA TUBULIN COMPLEX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19302:SF33">
        <property type="entry name" value="GAMMA-TUBULIN COMPLEX COMPONENT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04130">
        <property type="entry name" value="GCP_C_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17681">
        <property type="entry name" value="GCP_N_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3 5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gamma tubulin complex component protein N-terminal" evidence="8">
        <location>
            <begin position="213"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="domain" description="Gamma tubulin complex component C-terminal" evidence="7">
        <location>
            <begin position="604"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="117"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="131"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000031249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="895" mass="103106" checksum="00ACD061499E949B" modified="2012-04-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>H2XNN6</accession>
    <name>H2XNN6_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Ras-related protein Rab-36</fullName>
            <ecNumber evidence="3">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">wild type</strain>
        </source>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="15">Golgi apparatus membrane</location>
            <topology evidence="15">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="3"/>
    <dbReference type="EMBL" id="EAAA01002514">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XNN6"/>
    <dbReference type="FunCoup" id="H2XNN6">
        <property type="interactions" value="69"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000031269"/>
    <dbReference type="Ensembl" id="ENSCINT00000032683.1">
        <property type="protein sequence ID" value="ENSCINP00000031269.1"/>
        <property type="gene ID" value="ENSCING00000022926.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159687"/>
    <dbReference type="HOGENOM" id="CLU_041217_22_0_1"/>
    <dbReference type="InParanoid" id="H2XNN6"/>
    <dbReference type="OMA" id="FKCIAAA"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000707">
        <property type="entry name" value="RAB36, member RAS oncogene family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0636">Prenylation</keyword>
    <keyword evidence="9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="248"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00037794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00058763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00067830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000031269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="267" mass="30167" checksum="76AE99C8E6AD6199" modified="2012-04-18" version="1">MAESGYHSRIAQVHSDRVVTKFPEAFCKAARVDQKYDFDPRIRQSCSSERINNVSLKICKMIIIGDVSVGKTSLVNRFCKKAFDKDYKATIGVDFEVERFEILNTPFTLQMWDTAGQERFQCIAAAYYRGAHIVSIVFDLSDINTLASATKWLEAAQKENKGAGELLVFLVGTKKDLVAPQTYKEVEKEAIQVARQLNAEYWAVSAKTNEKVRDFFFRVASLSFEGTVLRELEKRQFTPVSQIGDGVSIQLNRNNPPPPRSSKTQCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>H2XNV1</accession>
    <name>H2XNV1_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Poly [ADP-ribose] polymerase</fullName>
            <shortName evidence="10">PARP</shortName>
            <ecNumber evidence="10">2.4.2.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">wild type</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the ARTD/PARP family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.-" evidence="10"/>
    <dbReference type="EMBL" id="EAAA01000789">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XNV1"/>
    <dbReference type="STRING" id="7719.ENSCINP00000031334"/>
    <dbReference type="Ensembl" id="ENSCINT00000030572.1">
        <property type="protein sequence ID" value="ENSCINP00000031334.1"/>
        <property type="gene ID" value="ENSCING00000021458.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000170301"/>
    <dbReference type="HOGENOM" id="CLU_416143_0_0_1"/>
    <dbReference type="InParanoid" id="H2XNV1"/>
    <dbReference type="OMA" id="THENTVM"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003950">
        <property type="term" value="F:NAD+ poly-ADP-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07997">
        <property type="entry name" value="WGR_PARP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.228.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.142.10">
        <property type="entry name" value="Poly(ADP-ribose) polymerase, regulatory domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050800">
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
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                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
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                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Chapman J."/>
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                <person name="De Tomaso A."/>
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                <person name="Di Gregorio A."/>
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                <person name="Hastings K.E."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
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                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
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                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="De Tomaso A."/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
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                <person name="Chapman J."/>
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                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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            <strain evidence="1">wild type</strain>
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            <authorList>
                <consortium name="Ensembl"/>
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        <source>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Makabe K.W."/>
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                <person name="Miura S."/>
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                <person name="Suzuki M.M."/>
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                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
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                <person name="Doggett N."/>
                <person name="Glavina T."/>
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                <person name="Richardson P."/>
                <person name="Lucas S."/>
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    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
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            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="3" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01002301">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161095"/>
    <dbReference type="HOGENOM" id="CLU_000288_18_6_1"/>
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        <property type="component" value="Chromosome 6"/>
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    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Leu-rich_rpt"/>
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        <property type="entry name" value="LRR_dom_sf"/>
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        <property type="entry name" value="SLRP"/>
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    <dbReference type="PANTHER" id="PTHR45712">
        <property type="entry name" value="AGAP008170-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45712:SF27">
        <property type="entry name" value="LRRNT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000033822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="331" mass="36875" checksum="916487A323DE447B" modified="2012-04-18" version="1">GCPRVCRCDERRKMVNCNERDLNFVPHGIPTNTKVLYLQHNNIGNSPSLEQNLSKLKVLEKLYIYSNKITSFPANLPSSIVYVSLRQNNIKFIGKTALRGLSNLRELHLDSNNITIEGLSEDAFSPAVNLQELIFTQNSLTHLPSGLPRSLISLRVANNRISDVTAASLSSLSKLIVLDFSNNELNDASFQDGSLKQLENLRTIDLSGNHITNIPTQLPGNITNLLLSRNKIQYIFAAKSEPGEIPIRGDLQSFPRLKKLDLSSNQLHSVEKGAFTNLPSAISVELHGNPWRCDCNLQYLKRWMTYTTSVVRSSEGNTRCATPAIFSDVTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>H2XWP4</accession>
    <name>H2XWP4_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Death domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01000187">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XWP4"/>
    <dbReference type="Ensembl" id="ENSCINT00000036832.1">
        <property type="protein sequence ID" value="ENSCINP00000034078.1"/>
        <property type="gene ID" value="ENSCING00000025086.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00660000097224"/>
    <dbReference type="HOGENOM" id="CLU_1128740_0_0_1"/>
    <dbReference type="InParanoid" id="H2XWP4"/>
    <dbReference type="OMA" id="LQMWLER"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000488">
        <property type="entry name" value="Death_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50017">
        <property type="entry name" value="DEATH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Death" evidence="2">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="107"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="149"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="169"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="191"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000034078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="282" mass="30879" checksum="C6B95E9F34442F3B" modified="2012-04-18" version="1">MLQMWLERSGDKATDEQLSAGLNSIGRGDLCYLLEDPVFHYGEVDPSLHMDRHLHYDTSGFNALTDELGSATPTPGHVSSPYPFTNRENRPQISTSTPTPAIKAENETVNGDLSVITPSTTGVDDDDDYNTPHFIYVADEDDGAQENNVEDHDQSSIKSEENAQRSRKSSSASSSTTSGEHSMTEDNMVADTKETAESVKSEENNDEERNGNNDLYQPGSSDTADVPEENVITSTTTIIRTIVVQTDSPQTVELPSPKIEGEDVVEVSREVHQEVVNNDDKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>H2XZ38</accession>
    <accession>A0A1W2WPD3</accession>
    <name>H2XZ38_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ribonuclease P protein subunit p21</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100182886</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">wild type</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the eukaryotic/archaeal RNase P protein component 4 family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01000352">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002131371.1">
        <property type="nucleotide sequence ID" value="XM_002131335.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XZ38"/>
    <dbReference type="STRING" id="7719.ENSCINP00000034922"/>
    <dbReference type="Ensembl" id="ENSCINT00000035312.1">
        <property type="protein sequence ID" value="ENSCINP00000034922.1"/>
        <property type="gene ID" value="ENSCING00000019032.1"/>
    </dbReference>
    <dbReference type="GeneID" id="100182886"/>
    <dbReference type="KEGG" id="cin:100182886"/>
    <dbReference type="GeneTree" id="ENSGT00390000003020"/>
    <dbReference type="HOGENOM" id="CLU_079140_2_1_1"/>
    <dbReference type="InParanoid" id="H2XZ38"/>
    <dbReference type="OMA" id="DPKHLLW"/>
    <dbReference type="OrthoDB" id="128536at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 1"/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
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                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
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                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="1">Belongs to the NIBP family.</text>
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    <dbReference type="OMA" id="SMENGIR"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <source>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Terry A."/>
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                <person name="Mount S."/>
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                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
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                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
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                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1">wild type</strain>
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    <reference evidence="1" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
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        </citation>
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            <authorList>
                <consortium name="Ensembl"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
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    <reference evidence="21" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
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                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
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                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Nonaka M."/>
                <person name="Putnam N."/>
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                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">wild type</strain>
        </source>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
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        <subcellularLocation>
            <location evidence="14">Postsynaptic cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="EAAA01001692">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="ENSCING00000019514.1"/>
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        <property type="component" value="Chromosome 3"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="PBP2_iGluR_ligand_binding"/>
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        <property type="entry name" value="Glutamate receptor, ionotropic, delta 1"/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
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                <person name="Shoguchi E."/>
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                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
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                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
                <consortium name="Ensembl"/>
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</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>H2Y0B0</accession>
    <name>H2Y0B0_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CUB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">wild type</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001786">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2Y0B0"/>
    <dbReference type="STRING" id="7719.ENSCINP00000035344"/>
    <dbReference type="Ensembl" id="ENSCINT00000034384.1">
        <property type="protein sequence ID" value="ENSCINP00000035344.1"/>
        <property type="gene ID" value="ENSCING00000020711.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155299"/>
    <dbReference type="HOGENOM" id="CLU_015228_5_2_1"/>
    <dbReference type="InParanoid" id="H2Y0B0"/>
    <dbReference type="OMA" id="TEIECAF"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000013">
        <property type="entry name" value="Membrane frizzled-related protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24251:SF30">
        <property type="entry name" value="MEMBRANE FRIZZLED-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24251">
        <property type="entry name" value="OVOCHYMASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003577946" description="CUB domain-containing protein" evidence="4">
        <location>
            <begin position="22"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="5">
        <location>
            <begin position="25"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="5">
        <location>
            <begin position="155"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000035344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="275" mass="30127" checksum="EA810B98AA7167A6" modified="2012-04-18" version="1">MFAITSTILFANLLILQQTFAQEQCGGTLTAKEGVFQTPHYPHEYDSNTHCEWLIEVGADQVVQLTFAEFDLDDNYERDNCPTDRVLVYDGDSSSSPALTGRLCGQETPPVMTSTGRHMKISFKAGEDSFGGNGFKATYKSLPAEDHQRCILNSCGADITDTSCMIQSPGYPSGYAANLNCTWTIRAPQGVGNVVFTFTEADFKRKVDGRCSLNNVTLYDGSNIKAPVLHEPSCHTEDNRLKTTVIRSTGPVLIINMRTSAASDIKHGFNGNFVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>H2Y0C5</accession>
    <name>H2Y0C5_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01002287">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2Y0C5"/>
    <dbReference type="Ensembl" id="ENSCINT00000032330.1">
        <property type="protein sequence ID" value="ENSCINP00000035359.1"/>
        <property type="gene ID" value="ENSCING00000018556.1"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3001572_0_0_1"/>
    <dbReference type="InParanoid" id="H2Y0C5"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="17"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000035359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="57" mass="6753" checksum="BA020AE6B82DA0FA" modified="2012-04-18" version="1">SKKVSHLSHPTISYNHILSFLLVLFIHVSSIVFSFWMLIISNKNNLNQLTLVNWFYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>H2Y0D8</accession>
    <name>H2Y0D8_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Death domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
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                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
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                <person name="Manohar C."/>
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                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
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                <person name="Nomoto H."/>
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                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
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                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
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                <person name="Richardson P."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
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            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">wild type</strain>
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            <authorList>
                <consortium name="Ensembl"/>
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            <strain evidence="1">wild type</strain>
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                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
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                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
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                <person name="Ho I."/>
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                <person name="Huang W."/>
                <person name="Kawashima T."/>
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                <person name="Martinez D."/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="19">wild type</strain>
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    <reference evidence="19" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="19" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
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                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
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                <person name="Miura S."/>
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                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
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                <person name="Sasaki A."/>
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                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
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                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
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                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Goodstein D.M."/>
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                <person name="Hastings K.E."/>
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                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
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                <person name="Ueno K."/>
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                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
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                <person name="Di Gregorio A."/>
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                <person name="Goodstein D.M."/>
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                <person name="Hastings K.E."/>
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                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
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                <person name="Zhang C."/>
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                <person name="Doggett N."/>
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                <person name="Richardson P."/>
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            <title>Genomewide surveys of developmentally relevant genes in Ciona intestinalis.</title>
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                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
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                <person name="Roe B.A."/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
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                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
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                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
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                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
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                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
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                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
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                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2003" name="Dev. Genes Evol." volume="213" first="211" last="212">
            <title>Genomewide surveys of developmentally relevant genes in Ciona intestinalis.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Satoh N."/>
            </authorList>
            <dbReference type="PubMed" id="12736827"/>
            <dbReference type="DOI" id="10.1007/s00427-003-0330-z"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="journal article" date="2004" name="Development" volume="131" first="4047" last="4058">
            <title>Gene expression profiles of transcription factors and signaling molecules in the ascidian embryo: towards a comprehensive understanding of gene networks.</title>
            <authorList>
                <person name="Imai K.S."/>
                <person name="Hino K."/>
                <person name="Yagi K."/>
                <person name="Satoh N."/>
                <person name="Satou Y."/>
            </authorList>
            <dbReference type="PubMed" id="15269171"/>
            <dbReference type="DOI" id="10.1242/dev.01270"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2005-04" db="EMBL/GenBank/DDBJ databases">
            <title>Expressed genes in Ciona intestinalis.</title>
            <authorList>
                <person name="Satou Y."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="9" key="5">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">wild type</strain>
        </source>
    </reference>
    <reference evidence="9" key="6">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4 5">Nucleus</location>
        </subcellularLocation>
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    <dbReference type="EMBL" id="EAAA01000422">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
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        <property type="protein sequence ID" value="BAE06499.1"/>
        <property type="molecule type" value="mRNA"/>
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    <dbReference type="AlphaFoldDB" id="Q4H3C8"/>
    <dbReference type="STRING" id="7719.ENSCINP00000014298"/>
    <dbReference type="Ensembl" id="ENSCINT00000014298.3">
        <property type="protein sequence ID" value="ENSCINP00000014298.3"/>
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        <property type="component" value="Chromosome 1"/>
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        <property type="evidence" value="ECO:0007669"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="homeodomain"/>
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        <property type="entry name" value="pancreas/duodenum homeobox protein 1"/>
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        <property type="entry name" value="Homeodomain-like"/>
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        <property type="entry name" value="HD"/>
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        <property type="entry name" value="HD_metazoa"/>
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        <property type="entry name" value="HOMEOTIC PROTEIN PROBOSCIPEDIA"/>
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    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="114"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
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    </evidence>
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        <source>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
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            <taxon>Phlebobranchia</taxon>
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    <reference evidence="5" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
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                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="gene ID" value="ENSCING00000000389.3"/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="De Tomaso A."/>
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                <person name="Hastings K.E."/>
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                <person name="Mochizuki Y."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
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                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="8" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="8">wild type</strain>
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    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
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        <text evidence="2">Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.</text>
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</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
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                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="Ensembl" id="ENSCINP00000004956.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="95" mass="11150" checksum="85AB51EBA0862C43" modified="2012-04-18" version="2">MGQLTILLLVVIVMIIVIAPTGTAQSSMPRYCTRLCKRNRYTGNYMKQVCKRKDRQKCYICRSAYKCGAIYDRIFRSPEVEPLNRKKIFQPIRQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>F6QHS0</accession>
    <name>F6QHS0_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">wild type</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01002531">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6QHS0"/>
    <dbReference type="STRING" id="7719.ENSCINP00000004926"/>
    <dbReference type="Ensembl" id="ENSCINT00000004926.3">
        <property type="protein sequence ID" value="ENSCINP00000004926.3"/>
        <property type="gene ID" value="ENSCING00000002420.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00970000196682"/>
    <dbReference type="HOGENOM" id="CLU_047314_0_0_1"/>
    <dbReference type="InParanoid" id="F6QHS0"/>
    <dbReference type="OMA" id="KCQPLQL"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990756">
        <property type="term" value="F:ubiquitin-like ligase-substrate adaptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
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        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
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        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19849:SF1">
        <property type="entry name" value="F-BOX_WD REPEAT-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19849">
        <property type="entry name" value="PHOSPHOLIPASE A-2-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="F-box" evidence="4">
        <location>
            <begin position="49"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="160"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="200"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="283"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="323"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="363"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000004926.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="394" mass="43712" checksum="25811444E8ED69DB" modified="2012-04-18" version="2">METDEEMIIGRFTKMERAEQDELLRKLLLKCKPRQLQMLYGDTRQLLAVDFVPFIPREIVDRIFSYLTPQELSRASCCSGQWRERANSSSLWKALCRRRSWLHFGEGSCQPQELFSSTPVTLAPMTVLTSPTFTPIINTCKANHLQKNWATGRYVVMPPMRGHKDRISCVDCAGNIVASGSDDHLVVLWDVSTGDKLHVFSHHNDAVTCVKIKNNLLLSGCADGVIRIYNISTGKCLGQLLDEHQAKSGVRFLCFDGTKAISGHDDKTVRIWAVLTGRCLYVMTGHTDDIASMVCFGKYAVTTSWDETVKVWDTESGVCAHTLIGHTEVVHCAHANDQYVVTGGGDNVVKVWLMGNGLCSQTLVGHTDDVYCVGFNAEIIASGSADSSVRDMEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F6QLS9</accession>
    <name>F6QLS9_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="F6QLS9"/>
    <dbReference type="STRING" id="7719.ENSCINP00000025226"/>
    <dbReference type="Ensembl" id="ENSCINT00000025472.2">
        <property type="protein sequence ID" value="ENSCINP00000025226.2"/>
        <property type="gene ID" value="ENSCING00000013827.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153772"/>
    <dbReference type="HOGENOM" id="CLU_109628_0_0_1"/>
    <dbReference type="InParanoid" id="F6QLS9"/>
    <dbReference type="OMA" id="DEEMITH"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051952">
        <property type="entry name" value="Golgi-autophagy_related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23157">
        <property type="entry name" value="GRIP AND COILED-COIL DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23157:SF25">
        <property type="entry name" value="GRIP AND COILED-COIL DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="46"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="119"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000025226.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="220" mass="25814" checksum="119A8C0F1E5006E8" modified="2012-04-18" version="2">MFKFNKNEELYDVIDKQKDQINRYEGRLRDLVHAYKSLQKEKEALEESMKALTTPSSGGGKKPSTLDDNPTALNEDESTEPDIQHLQSQLHTLTTSLSTMSDEKKKLVLVYQAEKKQFKHEHEVEVTKLEEETQVLLEKNKLLGEELTRVKEKLRNQQSDHEQEQSTHSIILREIQKMLNDERTKNESLELAMRDLNTATRVAEIEVQQHEDYKRRVRQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F6QPN8</accession>
    <name>F6QPN8_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">wild type</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002841">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6QPN8"/>
    <dbReference type="Ensembl" id="ENSCINT00000013582.3">
        <property type="protein sequence ID" value="ENSCINP00000013582.3"/>
        <property type="gene ID" value="ENSCING00000006614.3"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1758167_0_0_1"/>
    <dbReference type="InParanoid" id="F6QPN8"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002350">
        <property type="entry name" value="Kazal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036058">
        <property type="entry name" value="Kazal_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07648">
        <property type="entry name" value="Kazal_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100895">
        <property type="entry name" value="Kazal-type serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51465">
        <property type="entry name" value="KAZAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kazal-like" evidence="3">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="66"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="94"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000013582.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="148" mass="16371" checksum="2DF8247F49A2FEB7" modified="2012-04-18" version="2">MMNCIASGVMLLCGEDGHYYVNYCFITMRSCESQSEIKVHAVGQSSHCSPQGSPFAALLPENRIPGYDVCGMEDNHCHNFGICLKNIQQRICLCPRHYHGNRCQYRNAPLNNIDYALSRSNAIIGNPTSQVHGSPAIVLNSRSGYETS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>F6QSB8</accession>
    <name>F6QSB8_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">glutathione transferase</fullName>
            <ecNumber evidence="1">2.5.1.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100182061</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.5.1.18" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="F6QSB8"/>
    <dbReference type="STRING" id="7719.ENSCINP00000001243"/>
    <dbReference type="Ensembl" id="ENSCINT00000001243.3">
        <property type="protein sequence ID" value="ENSCINP00000001243.3"/>
        <property type="gene ID" value="ENSCING00000000677.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160278"/>
    <dbReference type="HOGENOM" id="CLU_039475_1_0_1"/>
    <dbReference type="InParanoid" id="F6QSB8"/>
    <dbReference type="OMA" id="IRIMFAE"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03039">
        <property type="entry name" value="GST_N_Sigma_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000258">
        <property type="entry name" value="Glutathione S-transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000030">
        <property type="entry name" value="Glutathione S-transferase S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050213">
        <property type="entry name" value="GST_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11571">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11571:SF150">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14497">
        <property type="entry name" value="GST_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01205">
        <property type="entry name" value="AMPS.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00363">
        <property type="entry name" value="AMPS_(cytGST):_Alpha-__Mu-__Pi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST N-terminal" evidence="2">
        <location>
            <begin position="5"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="3">
        <location>
            <begin position="72"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000001243.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="202" mass="22786" checksum="1955763D5512CF63" modified="2012-04-18" version="2">TKEMPSYKLSYFNGRGRAELIRIMFAEAGVKFVDDRITDNWPKRKAEFPFGQLPVLEIDGTQLSQTAAIVGYLAKKFNFLSEDDLTNGKVQEIIGIFGDCFAKLPFFEKDEKKKAAAVAEQVNTKIIPAFTKIQEKFVPDCSSTLLGEFTVADMALLTVVDSINDFDKTVFENFPKLKKVYNDTCARPKIAAYLKTRPETPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>F6QVW6</accession>
    <name>F6QVW6_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Methyltransferase-like protein 15</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">wild type</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily. RsmH family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002527">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="EAAA01002528">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6QVW6"/>
    <dbReference type="FunCoup" id="F6QVW6">
        <property type="interactions" value="98"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000001567"/>
    <dbReference type="Ensembl" id="ENSCINT00000001567.3">
        <property type="protein sequence ID" value="ENSCINP00000001567.3"/>
        <property type="gene ID" value="ENSCING00000000864.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000014756"/>
    <dbReference type="HOGENOM" id="CLU_038422_1_0_1"/>
    <dbReference type="InParanoid" id="F6QVW6"/>
    <dbReference type="OMA" id="NPAKRTF"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071424">
        <property type="term" value="F:rRNA (cytosine-N4-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070475">
        <property type="term" value="P:rRNA base methylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.170">
        <property type="entry name" value="Putative methyltransferase TM0872, insert domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01007">
        <property type="entry name" value="16SrRNA_methyltr_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002903">
        <property type="entry name" value="RsmH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023397">
        <property type="entry name" value="SAM-dep_MeTrfase_MraW_recog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00006">
        <property type="entry name" value="16S rRNA (cytosine(1402)-N(4))-methyltransferase RsmH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11265:SF0">
        <property type="entry name" value="12S RRNA N4-METHYLCYTIDINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11265">
        <property type="entry name" value="S-ADENOSYL-METHYLTRANSFERASE MRAW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01795">
        <property type="entry name" value="Methyltransf_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004486">
        <property type="entry name" value="MraW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81799">
        <property type="entry name" value="Putative methyltransferase TM0872, insert domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000001567.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="319" mass="36062" checksum="386341505BC26534" modified="2012-04-18" version="2">HIPVMAEELVSGLNIKRGDVIIDMTFGAGGHTKFIMENYKHCKVIALDRDEKAFEIAQDLSKKFKGRIIPLLGKFSELDKLLSENGIVENSLNAALLDAGCSSMQFDQPERGFSLVRDGPLDMRMDGNRSEGQITAADVVNNLDEEELTEIIYKYGDERNARNISSSIIENRPISTTLELARVVSMAFSQTSDVFRVDSLGRRCHIATKTFMAIRIFVNDELNEICHGIKTSEKYLMNGGKMGVITFHSLEDRVVKRMLKQIEISKPFNVKMGVGKRSKTERRYWKREQSLLLPSGEEIEQNPRSRSAKLRIATRCRDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>F6R384</accession>
    <name>F6R384_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PUM-HD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="F6R384"/>
    <dbReference type="STRING" id="7719.ENSCINP00000020346"/>
    <dbReference type="Ensembl" id="ENSCINT00000020346.3">
        <property type="protein sequence ID" value="ENSCINP00000020346.3"/>
        <property type="gene ID" value="ENSCING00000010208.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000004964"/>
    <dbReference type="HOGENOM" id="CLU_1137687_0_0_1"/>
    <dbReference type="InParanoid" id="F6R384"/>
    <dbReference type="OMA" id="NIWRQSS"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040000">
        <property type="entry name" value="NOP9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001313">
        <property type="entry name" value="Pumilio_RNA-bd_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13102">
        <property type="entry name" value="NUCLEOLAR PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13102:SF0">
        <property type="entry name" value="NUCLEOLAR PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22493">
        <property type="entry name" value="PUF_NOP9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000020346.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="240" mass="27773" checksum="73967A55BFB0B485" modified="2012-04-18" version="2">MLKIIHAGRPGVVVKLGEGCVRSPTTQSTFVTMVMKAFNCDDDEKKKLIVPLVASLSSYDKMFSKTFDPDKPPTFKVAYHGSLLLQTILKFDKIFVFVKSFLEIPSSHTVTLACSAPGSHLVDAFFSSEKVRAKRKLKWIEKTKDVFFKIAMDKYGSRVLENIWRQSSIKMKIIIAESLVPHESSLTNDQHGKHIFRKFAIRQFHQRNEDWKSFQVKIEKKRKMFQDFLPDEQQKKKKKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>F6RG51</accession>
    <name>F6RG51_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
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                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
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                <person name="Azumi K."/>
                <person name="Boore J."/>
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                <person name="Medina M."/>
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                <person name="Mount S."/>
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                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
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                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
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                <person name="Shin-i T."/>
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                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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    <reference evidence="12" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
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                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
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                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
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                <person name="Oishi K."/>
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                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="11" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
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                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="11">wild type</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <reaction evidence="8">
            <text>(6S)-5-methyl-5,6,7,8-tetrahydrofolate + NADP(+) = (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + NADPH + H(+)</text>
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            <dbReference type="Rhea" id="RHEA:19819"/>
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    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
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    <comment type="pathway">
        <text evidence="2 9">One-carbon metabolism; tetrahydrofolate interconversion.</text>
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    <comment type="similarity">
        <text evidence="3">Belongs to the methylenetetrahydrofolate reductase family.</text>
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    <dbReference type="STRING" id="7719.ENSCINP00000013160"/>
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        <property type="protein sequence ID" value="ENSCINP00000013160.3"/>
        <property type="gene ID" value="ENSCING00000006388.3"/>
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    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">wild type</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002650">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6S4S1"/>
    <dbReference type="FunCoup" id="F6S4S1">
        <property type="interactions" value="52"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000004484"/>
    <dbReference type="Ensembl" id="ENSCINT00000004484.3">
        <property type="protein sequence ID" value="ENSCINP00000004484.3"/>
        <property type="gene ID" value="ENSCING00000002198.3"/>
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    <dbReference type="GeneTree" id="ENSGT00940000154587"/>
    <dbReference type="HOGENOM" id="CLU_000288_63_0_1"/>
    <dbReference type="InParanoid" id="F6S4S1"/>
    <dbReference type="OMA" id="NKMTEVT"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009931">
        <property type="term" value="F:calcium-dependent protein serine/threonine kinase activity"/>
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        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0004683">
        <property type="term" value="F:calcium/calmodulin-dependent protein kinase activity"/>
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    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
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    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="CDD" id="cd14090">
        <property type="entry name" value="STKc_Mnk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001987">
        <property type="entry name" value="MAP kinase-interacting serine/threonine-protein kinase 1"/>
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    <dbReference type="FunFam" id="3.30.200.20:FF:000093">
        <property type="entry name" value="Putative map kinase-interacting serine/threonine-protein kinase 1"/>
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    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
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    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="93"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000004484.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="446" mass="49895" checksum="593B86143A17C153" modified="2012-04-18" version="2">MQSNTTDSGTGSLVRPSRATELLISEALNNNINSALDNQDGSNATSRISEPIEINQSKRLQVAAQAARQRRRKKRRTRAADSFCNGTFEDLYYINGELLGEGAYATVWTCINKYTHKEYAVKIIEKHVAGHSRMKVFKEVELLHQCAGRPNILQLIEYFETNEKFYLVFEKMYGGTLLHRIERCEHFDECEAARVVRDIATALAFLHDKGIAHRDLKPANILCETADKVSPVKICDFDLASGCRSSMMEPIKTPELSSPVGSAEYMAPEVVDTFVFEESLSYDKRCDIWSLGVITYIMLSGHPPFVGACSEDCGWDVGEACQTCQDMLFASIKSGEYSFPEEQWSGISNDAKDLISHLIVRDRSMRYNAHQILEHPWIKGTASETPLSTPQLLSRRDSSTHDLTQFAAHAVAYERKLACNDTSTITQGISNVRLSTPGTSALAQRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>F6SDR2</accession>
    <name>F6SDR2_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Zinc finger protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">wild type</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="EAAA01002021">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6SDR2"/>
    <dbReference type="Ensembl" id="ENSCINT00000024954.2">
        <property type="protein sequence ID" value="ENSCINP00000024708.2"/>
        <property type="gene ID" value="ENSCING00000013467.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000169957"/>
    <dbReference type="HOGENOM" id="CLU_630981_0_0_1"/>
    <dbReference type="InParanoid" id="F6SDR2"/>
    <dbReference type="OMA" id="HERGHYD"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18186">
        <property type="entry name" value="BTB_POZ_ZBTB_KLHL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000322">
        <property type="entry name" value="GDNF-inducible zinc finger protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZnFinger_BTB_dom_contain"/>
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        <property type="match status" value="3"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="26"/>
            <end position="102"/>
        </location>
    </feature>
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        <location>
            <begin position="294"/>
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        <location>
            <begin position="322"/>
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        <location>
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            <end position="377"/>
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        <location>
            <begin position="378"/>
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        <location>
            <begin position="406"/>
            <end position="435"/>
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            <begin position="154"/>
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        <location>
            <begin position="227"/>
            <end position="264"/>
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        <location>
            <begin position="233"/>
            <end position="251"/>
        </location>
    </feature>
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            <begin position="252"/>
            <end position="262"/>
        </location>
    </feature>
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        <source>
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50157"/>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000024708.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="435" mass="49434" checksum="5C370053E672D9C7" modified="2012-04-18" version="2">RDHVFYEFPSILMSQLNEQRLDGDNCDVTLIANDGVTFKAHKAVLSSYSRYFKMYFLTNQRNKQDILSPSVTFFYDVTGGFCSRTIGCILEAFYTSRLVLNIELVNKVLLAADVMEVYRVRNNSRKISRTKPSKGSKDKSAVACGETPPAFNVTFPARVHPEEPRPHTSAFKPRGLTQHTAVQPYPLPSVPPFITVPVSKSSTSYFWSFNYFQKQKKTDKKCVSIVHRAPNPTTSGNTESSSSDDSNITSSVRGTSPQQSSDIEIVDEVRRINTQKVPNRNEAVATDARHKRAYQCDLCNASFPSKAGLSVHFRLHTGERPYQCTICHKSFTQLGHVQRHQKVHTGEKPYQCGICHARVRDKASMRYHIQAHKGVKPFHCDQCDARFTKRSSLVKHKNVHTGEYMHQCQQCKASFREKASLSRHKRCQHSQHTDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>F6T2C1</accession>
    <name>F6T2C1_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">wild type</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the calmodulin family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001839">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6T2C1"/>
    <dbReference type="FunCoup" id="F6T2C1">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSCINT00000017572.3">
        <property type="protein sequence ID" value="ENSCINP00000017572.3"/>
        <property type="gene ID" value="ENSCING00000008615.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162930"/>
    <dbReference type="HOGENOM" id="CLU_061288_2_0_1"/>
    <dbReference type="InParanoid" id="F6T2C1"/>
    <dbReference type="OMA" id="ARKMKEC"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000527">
        <property type="entry name" value="Calmodulin-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050230">
        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF0">
        <property type="entry name" value="CALMODULIN LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0007">Acetylation</keyword>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="8"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="44"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="81"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="117"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000017572.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="149" mass="16833" checksum="0755533C7A126A7A" modified="2012-04-18" version="2">QADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGFISAAELRHVMTNLGEKLTDEEVDEMIREADVDGDGQVNYEEFVNMMTNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>F6TBT3</accession>
    <name>F6TBT3_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Amino acid permease/ SLC12A domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
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                <person name="Ueno K."/>
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                <person name="Matsumoto J."/>
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                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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                <person name="Meinertzhagen I.A."/>
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                <person name="Wang H.G."/>
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                <person name="Azumi K."/>
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    <sequence length="407" mass="46117" checksum="3E42D806643BD79D" modified="2012-04-18" version="2">MSKNDASMTSFSTIPEKTPEKLPNGESNQAFEDDDSVCKSDEKQQIETAIQELKEQITDDDHEDLYFDESKVNPVVKILISPVKMLEELFNRYKRFIPIIVVVIIFLGVTAYMVVALIWNFNRALTLMVIWLVVVAYNIYAFIRDYFGNDLMPYLKPVYKTVNKNMFWLQWVFVACVVGGLATWIAIDTSKRPEQLISGAGYLVFLFILFLTSKHPERVRWRPVFWGLLIQVCLGMFILRTQAGFDAFNWLGNIFQTFINYADEGARFLYGDDFADHYFAMKVLSIVIYFGAFISVMYYIGAMQWLIFKIAFLMQITLGTSATESMVAAGNIFVSMTESPLLIRPYINDLTTSELHAVMTAGFGTIAGSVLGAYLGFGIKAVYVIMACFMAAPCALAVSKLVYPETK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>F6TMY0</accession>
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        <recommendedName>
            <fullName evidence="5">EF-hand domain-containing protein</fullName>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <source>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
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            <fullName evidence="3">Uncharacterized protein</fullName>
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        <name type="scientific">Ciona intestinalis</name>
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    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="evidence" value="ECO:0007669"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EG:BACR7A4.14 PROTEIN-RELATED"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR43975">
        <property type="entry name" value="ZGC:101858"/>
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        <property type="entry name" value="adh_short"/>
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        <property type="entry name" value="GDHRDH"/>
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        <property type="entry name" value="SDRFAMILY"/>
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        <property type="entry name" value="ADH_SHORT"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000010325.3"/>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    </evidence>
    <sequence length="227" mass="24384" checksum="62CB6F32FF82274B" modified="2012-04-18" version="2">MASKFAGKVVLVTGASKGIGAEIAKQFANEGAVLSITGRDTAALSQVANECRAKQSNEVLEIVADLCDEEQAANVVQQTVEKLGKLDVLVNNAGICLPGKIEEATLKNFDQLFNINVRSMFHLTQLAVPHLKKVKGNIVNISSICSTGQYTRLLLYNMTKAAVDQFTKTIALELAPAGVRVNAANPASVKTTIYANAYTPKDLEAYYEKAKSFHPLGRNVEAKDVAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>F6U088</accession>
    <name>F6U088_CIOIN</name>
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        <recommendedName>
            <fullName evidence="12">Zyxin</fullName>
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    </protein>
    <organism evidence="16 17">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">wild type</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Focal adhesion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the zyxin/ajuba family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002381">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6U088"/>
    <dbReference type="STRING" id="7719.ENSCINP00000013640"/>
    <dbReference type="Ensembl" id="ENSCINT00000013640.3">
        <property type="protein sequence ID" value="ENSCINP00000013640.3"/>
        <property type="gene ID" value="ENSCING00000006648.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000168198"/>
    <dbReference type="HOGENOM" id="CLU_001357_10_3_1"/>
    <dbReference type="InParanoid" id="F6U088"/>
    <dbReference type="OMA" id="FPANANQ"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001725">
        <property type="term" value="C:stress fiber"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd09357">
        <property type="entry name" value="LIM3_Zyxin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000027">
        <property type="entry name" value="lipoma-preferred partner isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000057">
        <property type="entry name" value="Zyxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24212:SF8">
        <property type="entry name" value="LIM ZINC FINGER DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24212">
        <property type="entry name" value="ZYXIN/TRIP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="9" id="KW-0965">Cell junction</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10 13" id="KW-0440">LIM domain</keyword>
    <keyword evidence="5 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 13" id="KW-0862">Zinc</keyword>
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        <location>
            <begin position="248"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="15">
        <location>
            <begin position="308"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="15">
        <location>
            <begin position="369"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="99"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="148"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="174"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00039396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000013640.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="441" mass="48628" checksum="41CF380CB6739AA8" modified="2012-04-18" version="2">MLKFKVSNSDLFRSNYPEPKQAAKQSNYAMAGPSPVENPYAMAGPSKVTNTSPKAVSDVDKLTSQLLQGLDTGKRKKSDNIEDFNLPPPPNLDDFTFSPPSPIKSPDLPPPPPSAVPNVYSPLPPPPAPKPSSQMSKAAPPPVLPKPSKSTPLTKESKRGVNSITIQLNKPSSAPSPSPPAPTPTTAPAPEQKSAPYVPPASTRPSVGPGSRAGIPKDIKKNSEEELDFLTNNLLKNMENPRDEEFYGYCERCNGVVEGENVGCTAMDRTFHITCFTCLKCHRNLHGEQFYCVDKDPWCDKCYMNSLEKCTVCNETITDRILRATNKPYHPHCFQCVMCGKSLDGVPFTVDEKNEVHCVEDYHKKYAPRCSVCQEAIMPEKGKEETVRIVALERSFHVDCYKCEKCAVKLNSEKEGRGCFPLDGHIFCRDCNTKIIQHLSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>F6U1W2</accession>
    <name>F6U1W2_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
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                <person name="Inaba K."/>
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            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">wild type</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Podosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SH3PXD2 family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002192">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6UHP1"/>
    <dbReference type="FunCoup" id="F6UHP1">
        <property type="interactions" value="5"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000022853"/>
    <dbReference type="Ensembl" id="ENSCINT00000023099.2">
        <property type="protein sequence ID" value="ENSCINP00000022853.2"/>
        <property type="gene ID" value="ENSCING00000012164.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000168366"/>
    <dbReference type="HOGENOM" id="CLU_030529_1_0_1"/>
    <dbReference type="InParanoid" id="F6UHP1"/>
    <dbReference type="OMA" id="SHISQCE"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002102">
        <property type="term" value="C:podosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016176">
        <property type="term" value="F:superoxide-generating NADPH oxidase activator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042554">
        <property type="term" value="P:superoxide anion generation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06888">
        <property type="entry name" value="PX_FISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11856">
        <property type="entry name" value="SH3_p47phox_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12015">
        <property type="entry name" value="SH3_Tks_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1520.10:FF:000005">
        <property type="entry name" value="SH3 and PX domain-containing protein 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051228">
        <property type="entry name" value="NADPH_Oxidase/PX-Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037961">
        <property type="entry name" value="SH3PXD2_PX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15706:SF2">
        <property type="entry name" value="SH3 AND PX DOMAIN-CONTAINING PROTEIN 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15706">
        <property type="entry name" value="SH3 MULTIPLE DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07653">
        <property type="entry name" value="SH3_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0965">Cell junction</keyword>
    <keyword evidence="9" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="PX" evidence="13">
        <location>
            <begin position="7"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="12">
        <location>
            <begin position="154"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="12">
        <location>
            <begin position="223"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="12">
        <location>
            <begin position="357"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="294"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="300"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000022853.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="415" mass="46654" checksum="BCC1D3CADF2CC077" modified="2012-04-18" version="2">MSQIPKRLITAVNVNRAEKRRVPSKHYVYVIEVSWDDGSDTVIFRRYSKFFDLQISLLETFPKEGGMKDPSTRIIPFLPGKILFRRSNIRDVAMKRVLSIGEYCKDLIKLPSYIVQHSLILDFFETKPEDLKPPESESTSRASKRNSLDISQPILPESYVVVQDYIKTQPKELNARVGEVVEVMDKHENGWWFVSTEDGEQGWVPGVYLGKPDGKKYYSVFVHKSELYLTTTQYNGEDSEVSFNTGVLVEVLQKNLEGWWFVSYNGKQGWAPASYLTKPPESVTAISLSKKLSSPVKNDSSIPSSHSCTSLSSQSSSNAGDTFFSPASPSHKRLSGVGIKRPSLKPQVPPPPPPQHSDDVQYIAMHSFDGKIPHGVAFNIDDPVTVLSKSPGWWYVEVNGNEGWAPETYISKTTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>F6V437</accession>
    <accession>A0A1W2W870</accession>
    <name>F6V437_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="19 20">CDP-diacylglycerol--inositol 3-phosphatidyltransferase</fullName>
            <ecNumber evidence="5 20">2.7.8.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">LOC100185166</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">wild type</strain>
        </source>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Catalyzes the biosynthesis of phosphatidylinositol (PtdIns) as well as PtdIns:inositol exchange reaction. May thus act to reduce an excessive cellular PtdIns content. The exchange activity is due to the reverse reaction of PtdIns synthase and is dependent on CMP, which is tightly bound to the enzyme.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a CDP-1,2-diacyl-sn-glycerol + myo-inositol = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11580"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17268"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
            <dbReference type="ChEBI" id="CHEBI:58332"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="EC" id="2.7.8.11"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:11581"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="17">
            <dbReference type="Rhea" id="RHEA:11582"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 20 21">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.11" evidence="5 20"/>
    <dbReference type="EMBL" id="EAAA01000058">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002121442.1">
        <property type="nucleotide sequence ID" value="XM_002121406.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6V437"/>
    <dbReference type="FunCoup" id="F6V437">
        <property type="interactions" value="804"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000012669"/>
    <dbReference type="Ensembl" id="ENSCINT00000012669.3">
        <property type="protein sequence ID" value="ENSCINP00000012669.3"/>
        <property type="gene ID" value="ENSCING00000006135.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100185166"/>
    <dbReference type="KEGG" id="cin:100185166"/>
    <dbReference type="GeneTree" id="ENSGT00940000154169"/>
    <dbReference type="HOGENOM" id="CLU_067602_2_0_1"/>
    <dbReference type="InParanoid" id="F6V437"/>
    <dbReference type="OMA" id="AQTYSEN"/>
    <dbReference type="OrthoDB" id="10251079at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003881">
        <property type="term" value="F:CDP-diacylglycerol-inositol 3-phosphatidyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006661">
        <property type="term" value="P:phosphatidylinositol biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1760:FF:000003">
        <property type="entry name" value="CDP-diacylglycerol--inositol 3-phosphatidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000462">
        <property type="entry name" value="CDP-OH_P_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043130">
        <property type="entry name" value="CDP-OH_PTrfase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048254">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014387">
        <property type="entry name" value="CDP_diag_ino_3_P_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15362:SF4">
        <property type="entry name" value="CDP-DIACYLGLYCEROL--INOSITOL 3-PHOSPHATIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15362">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01066">
        <property type="entry name" value="CDP-OH_P_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000848">
        <property type="entry name" value="CDP_diag_ino_3_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00379">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 20" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="12 20" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15" id="KW-0464">Manganese</keyword>
    <keyword evidence="13 20 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14 20" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="16 20" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 20 21" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 22" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="141"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="166"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00050836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00057387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00070582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRNR" id="PIRNR000848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU003750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000012669.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="214" mass="24146" checksum="0C1AD6315E20F8CD" modified="2012-04-18" version="2">MATTVKDIMLFIPNLIGYVRIVLAIASAYYMPFDYVTASLCYVISVGLDAIDGYAARVFNQGTKFGAMLDQLTDRASTATLVMTLSYFYPKYMFLFQMSLVIDIICHWLHLHVSIMKGSSHKSMGLDSNPIMKVYYTSRPVLFFMCAGNELFYSMLYLLYFTEGPIVLGVSLFRVCLFVSFPIMAVKTLISIIHLMDASIRLCAIDADDRNKSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>F6V5G2</accession>
    <name>F6V5G2_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Kinesin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">wild type</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002687">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6V5G2"/>
    <dbReference type="STRING" id="7719.ENSCINP00000024348"/>
    <dbReference type="Ensembl" id="ENSCINT00000024594.2">
        <property type="protein sequence ID" value="ENSCINP00000024348.2"/>
        <property type="gene ID" value="ENSCING00000007350.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162166"/>
    <dbReference type="HOGENOM" id="CLU_001485_2_2_1"/>
    <dbReference type="InParanoid" id="F6V5G2"/>
    <dbReference type="OMA" id="CIGMSGV"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005871">
        <property type="term" value="C:kinesin complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47972:SF63">
        <property type="entry name" value="KINESIN FAMILY MEMBER 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47972">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KLP-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0505">Motor protein</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="7">
        <location>
            <begin position="1"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="168"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="311"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="169"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="188"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="338"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="34"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000024348.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="356" mass="39147" checksum="7DEAAA89CE64C148" modified="2012-04-18" version="2">VYGPDCSQNDIFDEIQPILTSFLDGYNACVVAYGQTGSGKTHTMLGNPHNPGIVPNSMNELFRLIEEKSTDSISLEVSVAEVYNNDIYDLLSDNPKQLKHDIVTTRGSSRDVPTLTQIKVKCPEDVESLMRLGLNRRAQIATNVHEHSSRSHLIVTVTAIVCQRDARPQSVSSNVSSEPNTPRRRPSSVDSTQGSLMHQSVPASPVTMPTKSMYKTKLQLVDLAGSECVGMSGVTGSALRESSFINRSLSALSDVLTALSERRSHIPYRNSKLTHLLQDSIGGDAKMMIMLCVSPTRKYLTETMQTLQFGSRARQVQRGPPRKRGTSPHATPRGESPMKSSSPRQIPQSVVRRVFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F6VE31</accession>
    <name>F6VE31_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
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                <person name="Di Gregorio A."/>
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                <person name="Harafuji N."/>
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                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
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                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
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                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
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                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
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                <person name="Sasaki A."/>
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                <person name="Tassy O."/>
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                <person name="Tokuoka M."/>
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                <person name="Richardson P."/>
                <person name="Lucas S."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
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            <strain evidence="1">wild type</strain>
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    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="1" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>F6VRH3</accession>
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        <recommendedName>
            <fullName evidence="9">Periodic tryptophan protein 2 homolog</fullName>
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        <name evidence="12" type="primary">LOC100183259</name>
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    <organism evidence="12 13">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
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            <taxon>Phlebobranchia</taxon>
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            <taxon>Ciona</taxon>
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    <reference evidence="13" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
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                <person name="Miura S."/>
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                <person name="Nomoto H."/>
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                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
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                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="12">wild type</strain>
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    <reference evidence="12" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <text evidence="7">Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome.</text>
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    <comment type="subunit">
        <text evidence="8">Part of the small subunit (SSU) processome, composed of more than 70 proteins and the RNA chaperone small nucleolar RNA (snoRNA) U3.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat PWP2 family.</text>
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    <dbReference type="OMA" id="VACEYIN"/>
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        <property type="component" value="Chromosome 14"/>
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    <dbReference type="GO" id="GO:0034388">
        <property type="term" value="C:Pwp2p-containing subcomplex of 90S preribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    </protein>
    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="F6W5H5"/>
    <dbReference type="Ensembl" id="ENSCINT00000028304.2">
        <property type="protein sequence ID" value="ENSCINP00000028058.2"/>
        <property type="gene ID" value="ENSCING00000016074.2"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1307321_0_0_1"/>
    <dbReference type="InParanoid" id="F6W5H5"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="192"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="65"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000028058.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="211" mass="24847" checksum="0D83B1EB94AD9FE4" modified="2012-04-18" version="2">MKQYYDDWKKTRRVKSFEEWKKQKQKEKRLNMMDNAQKESLQCVSEWMALDRQELAQSEFTKWRLRKESLSLERAQVELMEARELRKKAEQKARLKRAWLKARTMLNWKNITLPDKRNPLNKSDDTGKDSFKAIETNQSVNKLQLDEGFSEFSDSCVEDYDVKNVKSDSEPPGLLGELLKYDVSLNEVAEITPTHHDSSDDNAQVLAQDIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>F6W940</accession>
    <name>F6W940_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">wild type</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="EAAA01001081">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6W940"/>
    <dbReference type="Ensembl" id="ENSCINT00000026775.2">
        <property type="protein sequence ID" value="ENSCINP00000026529.2"/>
        <property type="gene ID" value="ENSCING00000014754.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000170273"/>
    <dbReference type="HOGENOM" id="CLU_675360_0_0_1"/>
    <dbReference type="InParanoid" id="F6W940"/>
    <dbReference type="OMA" id="CERIMIS"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.220.10">
        <property type="entry name" value="Leucine Aminopeptidase, subunit E, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043472">
        <property type="entry name" value="Macro_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051131">
        <property type="entry name" value="NEK_Ser/Thr_kinase_NIMA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44899">
        <property type="entry name" value="CAMK FAMILY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44899:SF8">
        <property type="entry name" value="NIMA-RELATED KINASE 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52949">
        <property type="entry name" value="Macro domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="28"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000026529.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="408" mass="45256" checksum="8C560A876EAF119E" modified="2011-07-27" version="1">MEAIQLLKFILDDIQAKGAVGTTINNQYHLEKLIGKGGFGAAYLAVDKKAPKNAPKKVLKRVMVETENIGNKSVVRTEAALLAKLSHPYIVKFHESFFDKNIFCIISEYCEGGDLADQIEKLEKSDRAFGDQMVEKIAYQLLCALNYLHTKKVLHRDLKPANVLLKGGDVKLTDFGVSKKLGVQEYLTKTLTGTILYVAPEILEEKPYSTKCDLWSLGVVFYEICTLKHPFESNIAAILTGSLPTLPNQAHQRILSKMLVRQTDQRCNAIDILKDPVFSNIRKNSDIEVRMVPRAEVNLFKGDITKENVDAIVIGVAKQDLTNLGNSPLAGAVLKAAGKELQDDCNASAASKPDMWSVVQVNSHQLPCIRVYIVLMEHYNENNSEKIKKSYSAAVKKCLENAEGNGFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>F6WA81</accession>
    <name>F6WA81_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phospholipase</fullName>
            <ecNumber evidence="7">3.1.4.4</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">wild type</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1,2-diacyl-sn-glycero-3-phosphate + choline + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14445"/>
            <dbReference type="ChEBI" id="CHEBI:15354"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="EC" id="3.1.4.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the phospholipase D family.</text>
    </comment>
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    <dbReference type="EMBL" id="EAAA01001234">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="Ensembl" id="ENSCINT00000026447.2">
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        <property type="gene ID" value="ENSCING00000002858.3"/>
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    <dbReference type="HOGENOM" id="CLU_000690_2_0_1"/>
    <dbReference type="InParanoid" id="F6WA81"/>
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    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 14"/>
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        <property type="term" value="C:plasma membrane"/>
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        <property type="term" value="F:phosphatidylinositol binding"/>
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        <property type="entry name" value="PLipase_D_euk"/>
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        <property type="entry name" value="Phospholipase_D_euk"/>
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        <property type="entry name" value="Phospholipase D/nuclease"/>
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    <keyword evidence="5 7" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="27"/>
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    </feature>
    <feature type="domain" description="PLD phosphodiesterase" evidence="8">
        <location>
            <begin position="482"/>
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</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
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    <reference evidence="18" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Membrane</location>
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    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.</text>
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    <dbReference type="HOGENOM" id="CLU_015171_2_0_1"/>
    <dbReference type="InParanoid" id="F6WTB4"/>
    <dbReference type="OMA" id="QMAEGNQ"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
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    <dbReference type="GO" id="GO:0043484">
        <property type="term" value="P:regulation of RNA splicing"/>
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        <property type="project" value="GO_Central"/>
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        <property type="entry name" value="Hephaestus, isoform C"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="2"/>
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    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
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    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15592">
        <property type="entry name" value="MATRIN 3/NUCLEAR PROTEIN 220-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13893">
        <property type="entry name" value="RRM_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="2">
        <location>
            <begin position="2"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="2">
        <location>
            <begin position="122"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000004050.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="199" mass="22948" checksum="17739DC1117D7EB6" modified="2012-04-18" version="2">TSVLHVSNLNEEDNKMVTPQSLFILFGVYGDVNRVKIMYQKKSNALVQMNDHQQAQTVIKYLHGVKLYDRPLQIMMSRHNQVQMPREGQEMAKLTQDYSNSPLHRFKKPGSKNFQNIFAPSEVLHLSNIPNDTEENFLRSKFEEVGVVVGFKFFVKDRRMALIQMSSLEEAILCLVNLHNLKLGESNHLRVSFSKGQIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>F6X4R3</accession>
    <name>F6X4R3_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">CENP-T/Histone H4 histone fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CENP-T/CNN1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="F6X4R3"/>
    <dbReference type="STRING" id="7719.ENSCINP00000027920"/>
    <dbReference type="Ensembl" id="ENSCINT00000028166.2">
        <property type="protein sequence ID" value="ENSCINP00000027920.2"/>
        <property type="gene ID" value="ENSCING00000018882.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000003044"/>
    <dbReference type="HOGENOM" id="CLU_1531995_0_0_1"/>
    <dbReference type="InParanoid" id="F6X4R3"/>
    <dbReference type="OMA" id="CHDIETM"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051382">
        <property type="term" value="P:kinetochore assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22920">
        <property type="entry name" value="HFD_CENP-T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028255">
        <property type="entry name" value="CENP-T"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035425">
        <property type="entry name" value="CENP-T/H4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46904">
        <property type="entry name" value="CENTROMERE PROTEIN T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46904:SF1">
        <property type="entry name" value="CENTROMERE PROTEIN T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15511">
        <property type="entry name" value="CENP-T_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CENP-T/Histone H4 histone fold" evidence="7">
        <location>
            <begin position="89"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF15511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000027920.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="177" mass="20434" checksum="69CD31E38370149E" modified="2012-04-18" version="2">MEDPLLVRSFHEDNLGDKPIQDATKVLMTSQSLNKSKQTKSSVKKKRPVPNMTRLVTAGQTSFHKNKKPTKRNLPVSRKQLYGIMQHYCANKVTKGAMDLVEECFEKFFKQLCHDIETMTLHGGRKVVTMADIELVMRRQRFVTDEKPLQIIIEENFPLEYRRLFIPVAGSVANKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>F6XI46</accession>
    <name>F6XI46_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Tolloid-like protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100176530</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">wild type</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001485">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6XI46"/>
    <dbReference type="STRING" id="7719.ENSCINP00000021911"/>
    <dbReference type="Ensembl" id="ENSCINT00000022157.2">
        <property type="protein sequence ID" value="ENSCINP00000021911.2"/>
        <property type="gene ID" value="ENSCING00000011480.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166030"/>
    <dbReference type="HOGENOM" id="CLU_015228_7_1_1"/>
    <dbReference type="InParanoid" id="F6XI46"/>
    <dbReference type="OMA" id="CKININA"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000013">
        <property type="entry name" value="Membrane frizzled-related protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000005">
        <property type="entry name" value="Procollagen C-endopeptidase enhancer 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24251:SF30">
        <property type="entry name" value="MEMBRANE FRIZZLED-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24251">
        <property type="entry name" value="OVOCHYMASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003346040" evidence="4">
        <location>
            <begin position="23"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="5">
        <location>
            <begin position="28"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="5">
        <location>
            <begin position="148"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="5">
        <location>
            <begin position="271"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="5">
        <location>
            <begin position="387"/>
            <end position="500"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000021911.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="500" mass="55735" checksum="8C5819FA668C6D1D" modified="2012-04-18" version="2">MKQVLLSVLIITLLSDIPNVVADSLFNLTGVVGSTSPYYTSPGHPGNNPHNLNTYYRINGRSSSNVITVSFPFFQLEKHRLCNFDSLAIYEGPTTTARLIGKYCGTKSFFTVTGRGQYMYMNYRTDRNIHSIGFRFRFSIAASNAPSCDQLSIGGGPNSGNITSPNYPLNYPHNTDCRYRINAGSATRIITLLFTAFNLESHIDCKYDFVEVHTGFKGNWSPTSKKYCGSAVPARQVITGQQYVYIRFFADRSVARSGFQMRYIISGGGSCDQLHLGGEPGSNGVIFSPNHPSDYPKNIMCRYKVSSSCHNNTIHFTFNAFNIERNAQCRWDYVEMFSNCTVTPVSLGRYCGSNVPDPITRRTACMSMKFKTDSSVTMSGFNITYTISGSCYGSTGVISSPSYPMNYPNFAQNNYVVKPPNATLIRLGFNDFDMEGTESNNCRFDSLTIRQGNETGPILSKFCGFLGSFTREYAATKLTLMFKTDRSVTRRGFQCVYTKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>F6XKP0</accession>
    <name>F6XKP0_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="F6XKP0"/>
    <dbReference type="FunCoup" id="F6XKP0">
        <property type="interactions" value="837"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000007040"/>
    <dbReference type="Ensembl" id="ENSCINT00000007040.3">
        <property type="protein sequence ID" value="ENSCINP00000007040.3"/>
        <property type="gene ID" value="ENSCING00000003421.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01020000230346"/>
    <dbReference type="HOGENOM" id="CLU_000688_2_4_1"/>
    <dbReference type="InParanoid" id="F6XKP0"/>
    <dbReference type="OMA" id="NKISHEH"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008540">
        <property type="term" value="C:proteasome regulatory particle, base subcomplex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036402">
        <property type="term" value="F:proteasome-activating activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000009">
        <property type="entry name" value="26S protease regulatory subunit 6A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000076">
        <property type="entry name" value="26S protease regulatory subunit 6A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000037">
        <property type="entry name" value="26S protease regulatory subunit 6A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050221">
        <property type="entry name" value="26S_Proteasome_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032501">
        <property type="entry name" value="Prot_ATP_ID_OB_2nd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23073">
        <property type="entry name" value="26S PROTEASOME REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16450">
        <property type="entry name" value="Prot_ATP_ID_OB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0647">Proteasome</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="10">
        <location>
            <begin position="177"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000007040.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="397" mass="44614" checksum="80EC2128A1E09D9E" modified="2012-04-18" version="2">EEVLKMSTNEISSRTKLLDNEIKIMKSETMRIGHELQSMKEKIKENAEKIKVNKTLPYLVSNVIEVSNIDLDSQRKGKCAVIKTSTRQTYFLPIMGLVEPEDLTPGDLVGVNKDSYLILEKLPTEYDSRVKAMEVDERPTEQYSDIGGLDKQIQELIEAVVLPMTHKDRFESIGIQPPKGVLMYGPPGTGKTLLARACAAQTKSTFLKLAGPQLVQMFIGDGAKLVRDAFALAKERKPAIIFIDELDAIGTKRFDSEKAGDREVQRTMLELLNQLDGFEPYNDIKVIAATNRVDILDPALLRSGRLDRKIEFPVPNEEARARILQIHSRKMNVSADVNFEELARCTDDFNGAQLKAVCVEAGMIALRREAIDLTHEDYMEGILEVQAKKKANLQYYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>F6Y025</accession>
    <name>F6Y025_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 11">Histidine ammonia-lyase</fullName>
            <ecNumber evidence="4 11">4.3.1.3</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9 11">
            <text>L-histidine = trans-urocanate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:21232"/>
            <dbReference type="ChEBI" id="CHEBI:17771"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
            <dbReference type="EC" id="4.3.1.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Amino-acid degradation; L-histidine degradation into L-glutamate; N-formimidoyl-L-glutamate from L-histidine: step 1/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the PAL/histidase family.</text>
    </comment>
    <dbReference type="EC" id="4.3.1.3" evidence="4 11"/>
    <dbReference type="AlphaFoldDB" id="F6Y025"/>
    <dbReference type="FunCoup" id="F6Y025">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000013857"/>
    <dbReference type="Ensembl" id="ENSCINT00000013857.3">
        <property type="protein sequence ID" value="ENSCINP00000013857.3"/>
        <property type="gene ID" value="ENSCING00000006739.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000009047"/>
    <dbReference type="HOGENOM" id="CLU_014801_4_0_1"/>
    <dbReference type="InParanoid" id="F6Y025"/>
    <dbReference type="OMA" id="YSLRCMP"/>
    <dbReference type="UniPathway" id="UPA00379">
        <property type="reaction ID" value="UER00549"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004397">
        <property type="term" value="F:histidine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060218">
        <property type="term" value="P:hematopoietic stem cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006548">
        <property type="term" value="P:L-histidine catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019556">
        <property type="term" value="P:L-histidine catabolic process to glutamate and formamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019557">
        <property type="term" value="P:L-histidine catabolic process to glutamate and formate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00332">
        <property type="entry name" value="PAL-HAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.275.10:FF:000008">
        <property type="entry name" value="Histidine ammonia-lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.200.10:FF:000003">
        <property type="entry name" value="Histidine ammonia-lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.200.10">
        <property type="entry name" value="Fumarase/aspartase (Central domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="entry name" value="Fumarase/aspartase (N-terminal domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001106">
        <property type="entry name" value="Aromatic_Lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005921">
        <property type="entry name" value="HutH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022313">
        <property type="entry name" value="Phe/His_NH3-lyase_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01225">
        <property type="entry name" value="hutH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006871">
        <property type="entry name" value="PRK09367.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10362">
        <property type="entry name" value="HISTIDINE AMMONIA-LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00221">
        <property type="entry name" value="Lyase_aromatic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="L-aspartase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00488">
        <property type="entry name" value="PAL_HISTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 11" id="KW-0369">Histidine metabolism</keyword>
    <keyword evidence="8 10" id="KW-0456">Lyase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000013857.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="639" mass="69581" checksum="B52AE4F87598CDE3" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>F6YQB2</accession>
    <name>F6YQB2_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nitric oxide synthase-interacting protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">E3 ubiquitin-protein ligase NOSIP</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">RING-type E3 ubiquitin transferase NOSIP</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100180053</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the NOSIP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="F6YQB2"/>
    <dbReference type="FunCoup" id="F6YQB2">
        <property type="interactions" value="481"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000007931"/>
    <dbReference type="Ensembl" id="ENSCINT00000007931.3">
        <property type="protein sequence ID" value="ENSCINP00000007931.3"/>
        <property type="gene ID" value="ENSCING00000015390.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015505"/>
    <dbReference type="HOGENOM" id="CLU_053742_0_0_1"/>
    <dbReference type="InParanoid" id="F6YQB2"/>
    <dbReference type="OMA" id="PCVTKFM"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16661">
        <property type="entry name" value="RING-Ubox1_NOSIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16662">
        <property type="entry name" value="RING-Ubox2_NOSIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:001144">
        <property type="entry name" value="Nitric oxide synthase-interacting protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016818">
        <property type="entry name" value="NOSIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031790">
        <property type="entry name" value="Znf-NOSIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13063">
        <property type="entry name" value="ENOS INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13063:SF10">
        <property type="entry name" value="NITRIC OXIDE SYNTHASE-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15906">
        <property type="entry name" value="zf-NOSIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF023577">
        <property type="entry name" value="ENOS_interacting"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nitric oxide synthase-interacting protein zinc-finger" evidence="10">
        <location>
            <begin position="4"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="85"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00029661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00032934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR023577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF15906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000007931.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="300" mass="34013" checksum="CDE10A2817B0A8A7" modified="2012-04-18" version="2">MTRHGKNATASSVYSYHEKQKDTEQSSYGTQAARLGKDSVKDFDACCLTLQPCRSPVVTPHGYLYEKEAILEYIVHKKRLLAKQSKLYDKQKKQEEKEMKELAQAEHRSKVEKFMKHEKSIVSKSNEAFKLKTEEKTAPVEIKPSTSQVFDKYDKNVPSFWIPALTPQAKEAKIKKPDSKVYCPMSNKPIKMKDLIPVKFTPIADRDKKTSMISKKDRWMCAVTHDTLGNSVPSVVLRPTGDVVTLECVDKIIRKNGMRHPVTDEVMSENDIIPLTRGGTGFASTNEVLDSKSYRPVIMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>F6YR14</accession>
    <name>F6YR14_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Solute carrier family 2, facilitated glucose transporter member 5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Fructose transporter</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Glucose transporter type 5, small intestine</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">wild type</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-fructose(out) = D-fructose(in)</text>
            <dbReference type="Rhea" id="RHEA:60372"/>
            <dbReference type="ChEBI" id="CHEBI:37721"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <location evidence="2">Sarcolemma</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002426">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="EAAA01002427">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6YR14"/>
    <dbReference type="STRING" id="7719.ENSCINP00000007910"/>
    <dbReference type="Ensembl" id="ENSCINT00000007910.3">
        <property type="protein sequence ID" value="ENSCINP00000007910.3"/>
        <property type="gene ID" value="ENSCING00000003814.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156846"/>
    <dbReference type="HOGENOM" id="CLU_001265_30_11_1"/>
    <dbReference type="InParanoid" id="F6YR14"/>
    <dbReference type="OMA" id="TQHFQRM"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042383">
        <property type="term" value="C:sarcolemma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055056">
        <property type="term" value="F:D-glucose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005353">
        <property type="term" value="F:fructose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046323">
        <property type="term" value="P:D-glucose import"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070837">
        <property type="term" value="P:dehydroascorbic acid transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990539">
        <property type="term" value="P:fructose import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:001511">
        <property type="entry name" value="Solute carrier family 2, facilitated glucose transporter member 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045263">
        <property type="entry name" value="GLUT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23503">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23503:SF132">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 2, FACILITATED GLUCOSE TRANSPORTER MEMBER 5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="9 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6 14" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="45"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="131"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="170"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="197"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="226"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="266"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="294"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="16">
        <location>
            <begin position="1"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00015973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000007910.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="356" mass="39141" checksum="FD1B69394FB27E85" modified="2012-04-18" version="2">MYIGEISPKQWRGAIGVLNQLHITIGILVAQVLGLQGMLGNEHTWPILFAFTCVPSIIQLMAIPFMPKSPRFLLIDEGKEDEARNVLVKLRGTDNVVSEMDEMRAEASAQSADGQLSIPQLFRDRSVRWQLITVLLMMAAQQLSGINAIFFYSNKIFSKAGIPAGKQQDLASVGVGVVNVLMTVISVGVIEWAGRKALIVWGFGMMIFWCAAMTVVLSLLSLNLTWISYLSIACMIGYIVGFAIGPGPIPWLITAELFRQSARPPAFMVSCLLNWTCNFIIGISFPAIADATGAYVFILFMFVCIGITVFLAIIMPETKGKTFQEISNLFAKRKSEGTAHPTESVNGKIYHKIQLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>F6YTY3</accession>
    <name>F6YTY3_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Exportin-6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100187212</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">wild type</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the exportin family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002908">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
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            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
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    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="entry name" value="LMNTD1"/>
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        <property type="entry name" value="LAMIN TAIL DOMAIN CONTAINING 1"/>
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        <property type="entry name" value="LAMIN TAIL DOMAIN-CONTAINING PROTEIN 1"/>
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    <proteinExistence type="predicted"/>
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        <location>
            <begin position="181"/>
            <end position="212"/>
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        <location>
            <begin position="63"/>
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        <location>
            <begin position="200"/>
            <end position="212"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000022440.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
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        <name type="scientific">Ciona intestinalis</name>
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    <reference evidence="17" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
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                <person name="Inaba K."/>
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            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">wild type</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endohydrolysis of (1-&gt;4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1-&gt;4)-alpha-linked D-glucose units.</text>
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        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>chloride</name>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the glycosyl hydrolase 13 family.</text>
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    <dbReference type="Ensembl" id="ENSCINT00000019596.3">
        <property type="protein sequence ID" value="ENSCINP00000019596.3"/>
        <property type="gene ID" value="ENSCING00000009641.3"/>
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    <dbReference type="InParanoid" id="F6ZTX2"/>
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        <property type="component" value="Chromosome 5"/>
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    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Golgi alpha-mannosidase II"/>
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        <property type="entry name" value="A-amylase_C"/>
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        <property type="entry name" value="Alpha_amylase"/>
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        <property type="entry name" value="GH13_cat_dom"/>
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    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000019596.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="506" mass="55212" checksum="A859126DE7F669AD" modified="2012-04-18" version="2">MKTVLLIFSLVAAASAQWDPNFVSGRAGIVHLFEWHWDTIADECETFLAPNLYAGVQVSPPNENRIVGGRPWWERYQPVSYKIGTRSGDRAAFENMVDRCNSVGVRIYVDAVVNHMCGGDGGSSTQGTAGSTCYPDSEGFPAVPYSSLDFNDGNCNTASGDIENYGDAAQVRNCKLVGLLDLNQGTDYVRGRIIEFMNDMLSLGVAGFRVDACKHMWPGDLEVIFGSLNDLSTAKGFSGGSRPYIYQEVIDQGGEPITADQYFSCGAVTEFKYGVNLGNNKNSIQYLENFGESWGMMPGGNALVFLNNHDNQRGHGGGGNIITFEDPFNLKIFTAYMMAWPYGVPRVMSSYYFSMYICYGKDFNCNIGSNFNSDEGPPSNQRSINADGSCGNGWVCEHRWRQIKNMMGFAVAAQGQGVSNWWSNGQQIAFSRGDRAFLAINHDGYTLSENLQTGMPAGDYCNVNSGEYSNNQCTGECISVGSDGYAYINIGATEDPVVAIHVNAPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>F6ZVK3</accession>
    <name>F6ZVK3_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01001171">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6ZVK3"/>
    <dbReference type="Ensembl" id="ENSCINT00000026673.1">
        <property type="protein sequence ID" value="ENSCINP00000026427.1"/>
        <property type="gene ID" value="ENSCING00000014686.1"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1291521_0_0_1"/>
    <dbReference type="InParanoid" id="F6ZVK3"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000026427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="214" mass="23589" checksum="7AFB9749B176EBD0" modified="2011-07-27" version="1">MRSGMTSPQSRMTSPKLSPIPSAKQLEEIKDGFEYIASNAKVSHLKRKCLERDQILAKLVLRLKKKRADTSLGVNDDDSTTSSVGFESILSKADRLLRSNDVTNDFSDTGRHIDVESAENIENKSYSDVSRTSPLIKALTEKSPLSQPRSPSHRIYVAKHDFAPANKPPSPAYNESLPITHSLLPDLPLLAGDTVVAIGNPDERESGKFYQLAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>F6ZYU1</accession>
    <accession>A0A1W2W327</accession>
    <name>F6ZYU1_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glutathione S-transferase omega</fullName>
            <shortName evidence="5">GSTO</shortName>
            <ecNumber evidence="5">1.20.4.2</ecNumber>
            <ecNumber evidence="5">1.8.5.1</ecNumber>
            <ecNumber evidence="5">2.5.1.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Glutathione-dependent dehydroascorbate reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Monomethylarsonic acid reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100179669</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">wild type</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Exhibits glutathione-dependent thiol transferase activity. Has high dehydroascorbate reductase activity and may contribute to the recycling of ascorbic acid. Participates in the biotransformation of inorganic arsenic and reduces monomethylarsonic acid (MMA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 5">
            <text>L-dehydroascorbate + 2 glutathione = glutathione disulfide + L-ascorbate</text>
            <dbReference type="Rhea" id="RHEA:24424"/>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:58297"/>
            <dbReference type="ChEBI" id="CHEBI:58539"/>
            <dbReference type="EC" id="1.8.5.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 5">
            <text>methylarsonate + 2 glutathione + H(+) = methylarsonous acid + glutathione disulfide + H2O</text>
            <dbReference type="Rhea" id="RHEA:15969"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17826"/>
            <dbReference type="ChEBI" id="CHEBI:33409"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:58297"/>
            <dbReference type="EC" id="1.20.4.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the GST superfamily. Omega family.</text>
    </comment>
    <dbReference type="EC" id="1.20.4.2" evidence="5"/>
    <dbReference type="EC" id="1.8.5.1" evidence="5"/>
    <dbReference type="EC" id="2.5.1.18" evidence="5"/>
    <dbReference type="EMBL" id="EAAA01001477">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002122925.1">
        <property type="nucleotide sequence ID" value="XM_002122889.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6ZYU1"/>
    <dbReference type="FunCoup" id="F6ZYU1">
        <property type="interactions" value="22"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000017658"/>
    <dbReference type="Ensembl" id="ENSCINT00000017658.3">
        <property type="protein sequence ID" value="ENSCINP00000017658.3"/>
        <property type="gene ID" value="ENSCING00000008645.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100179669"/>
    <dbReference type="KEGG" id="cin:100179669"/>
    <dbReference type="GeneTree" id="ENSGT00940000163896"/>
    <dbReference type="HOGENOM" id="CLU_011226_9_2_1"/>
    <dbReference type="InParanoid" id="F6ZYU1"/>
    <dbReference type="OMA" id="WYTDYSP"/>
    <dbReference type="OrthoDB" id="4951845at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045174">
        <property type="term" value="F:glutathione dehydrogenase (ascorbate) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050610">
        <property type="term" value="F:methylarsonate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000009">
        <property type="entry name" value="Glutathione S-transferase omega-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000123">
        <property type="entry name" value="Glutathione transferase o1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
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                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
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                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
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                <person name="Lindquist E."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Goodstein D.M."/>
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                <person name="Hastings K.E."/>
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                <person name="Meinertzhagen I.A."/>
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                <person name="Putnam N."/>
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                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
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                <person name="Shin-i T."/>
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                <person name="Stainier D."/>
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                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
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                <person name="Doggett N."/>
                <person name="Glavina T."/>
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                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">wild type</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="EAAA01000419">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000014406"/>
    <dbReference type="Ensembl" id="ENSCINT00000014406.3">
        <property type="protein sequence ID" value="ENSCINP00000014406.3"/>
        <property type="gene ID" value="ENSCING00000020548.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163795"/>
    <dbReference type="HOGENOM" id="CLU_058132_4_0_1"/>
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    <dbReference type="OMA" id="RIATMIM"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 1"/>
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        <property type="evidence" value="ECO:0000318"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:procollagen-proline 4-dioxygenase activity"/>
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        <property type="project" value="GO_Central"/>
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        <property type="entry name" value="Prolyl 4-hydroxylase subunit alpha 2"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
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        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
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    <dbReference type="InterPro" id="IPR045054">
        <property type="entry name" value="P4HA-like"/>
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    <dbReference type="InterPro" id="IPR006620">
        <property type="entry name" value="Pro_4_hyd_alph"/>
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    <dbReference type="InterPro" id="IPR044862">
        <property type="entry name" value="Pro_4_hyd_alph_FE2OG_OXY"/>
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        <property type="entry name" value="PROLYL 4-HYDROXYLASE SUBUNIT ALPHA-2"/>
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        <property type="entry name" value="2OG-FeII_Oxy_3"/>
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    </dbReference>
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        <property type="entry name" value="P4Hc"/>
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    </dbReference>
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        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
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    <keyword evidence="4" id="KW-0223">Dioxygenase</keyword>
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    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0847">Vitamin C</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="7">
        <location>
            <begin position="83"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000014406.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="201" mass="22905" checksum="3A63CF66EA92F1FC" modified="2012-04-18" version="2">XELWHSPHLVRFHGIMSDKEMAMIKSLAKPRLRRATVQNPVTGVLEFAHYRVSKSAWLKDEDHPVIKRVCQRISDVTGLSMETAEELQIANYGVGGQYEPHFDYSREVGNRIATFLTYMSNVEQGGSTVFLHPGIAVRPIKGSAVFWYNLLPSGAGDERTRHAACPVLTGVKWVSNKWIHERDQEFRRSCKTNIKADNRIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>F7AEZ3</accession>
    <name>F7AEZ3_CIOIN</name>
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        <recommendedName>
            <fullName evidence="3 7">Prolyl endopeptidase</fullName>
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        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100187168</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">wild type</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of Pro-|-Xaa &gt;&gt; Ala-|-Xaa in oligopeptides.</text>
            <dbReference type="EC" id="3.4.21.26"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the peptidase S9A family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.-" evidence="7"/>
    <dbReference type="EMBL" id="EAAA01001628">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F7AEZ3"/>
    <dbReference type="FunCoup" id="F7AEZ3">
        <property type="interactions" value="436"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000019289"/>
    <dbReference type="Ensembl" id="ENSCINT00000019289.3">
        <property type="protein sequence ID" value="ENSCINP00000019289.3"/>
        <property type="gene ID" value="ENSCING00000009474.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063426"/>
    <dbReference type="HOGENOM" id="CLU_011290_1_1_1"/>
    <dbReference type="InParanoid" id="F7AEZ3"/>
    <dbReference type="OMA" id="LDPWFSH"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070012">
        <property type="term" value="F:oligopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.120">
        <property type="entry name" value="Prolyl oligopeptidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002471">
        <property type="entry name" value="Pept_S9_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023302">
        <property type="entry name" value="Pept_S9A_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003653">
        <property type="entry name" value="Peptidase_C48_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002470">
        <property type="entry name" value="Peptidase_S9A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051167">
        <property type="entry name" value="Prolyl_oligopep/macrocyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42881">
        <property type="entry name" value="PROLYL ENDOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42881:SF2">
        <property type="entry name" value="PROLYL ENDOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02897">
        <property type="entry name" value="Peptidase_S9_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00862">
        <property type="entry name" value="PROLIGOPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50993">
        <property type="entry name" value="Peptidase/esterase 'gauge' domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PRO_ENDOPEP_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50600">
        <property type="entry name" value="ULP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 7" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="Ubiquitin-like protease family profile" evidence="8">
        <location>
            <begin position="154"/>
            <end position="587"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016310"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU368024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000019289.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="709" mass="80656" checksum="F6CDD8B1FD26754D" modified="2025-10-08" version="3"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>F7AN06</accession>
    <name>F7AN06_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
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                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
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                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
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                <person name="Huang W."/>
                <person name="Kawashima T."/>
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                <person name="Martinez D."/>
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                <person name="Wang H.G."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1">wild type</strain>
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <property type="entry name" value="P-loop_NTPase"/>
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        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
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        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    <sequence length="264" mass="29851" checksum="6B96DF256E8B061C" modified="2012-04-18" version="2">MKVIVAGFSKTGTKTMQAALTELGYNVYDYMENFEFLEKEWKQICTVGGSTELFRKMYENVDAVTDLPGCAFWDEILKAFPDAKIVLTMRDEEQWWKSMQKQHEENGSLIMQMIVNISPTGRRFGQHGIRILQSVVGDTMALTSTKINETLFKMAYRRHNAHVLQNAPKDQLLVFNFKDGWEPLCKFLGVPVPDTPFHHKNKKGAITKEILATNKVAIKMQREAMVVAAVSGVLAATGLYKGISLARTVNWADTFTTLSSLFKK</sequence>
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        <submittedName>
            <fullName evidence="3">Regulator of G-protein signaling 16-like</fullName>
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        <name evidence="3" type="primary">LOC100181578</name>
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        <name type="scientific">Ciona intestinalis</name>
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            <taxon>Eukaryota</taxon>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_002121843.4"/>
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    <dbReference type="KEGG" id="cin:100181578"/>
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        <property type="component" value="Chromosome 9"/>
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        <property type="entry name" value="Putative regulator of g-protein signaling 12"/>
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        <property type="entry name" value="RGS_subdomain_2"/>
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        <property type="entry name" value="REGULATOR OF G PROTEIN SIGNALING"/>
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        <property type="entry name" value="RGS DOMAIN-CONTAINING PROTEIN-RELATED"/>
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    </evidence>
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    </evidence>
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        <source>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
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                <person name="Haga S."/>
                <person name="Hayashi H."/>
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            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <location>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Tunicata</taxon>
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            <taxon>Phlebobranchia</taxon>
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            <taxon>Ciona</taxon>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">wild type</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <text evidence="11">Part of the dynein complex of respiratory cilia.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Consists of at least two heavy chains and a number of intermediate and light chains. Interacts with BICD2. Interacts with CFAP45 and CFAP52. Interacts with CFAP53.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dynein intermediate chain family.</text>
    </comment>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="ENSCINP00000017986.3"/>
        <property type="gene ID" value="ENSCING00000008832.3"/>
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    <dbReference type="HOGENOM" id="CLU_015820_2_0_1"/>
    <dbReference type="InParanoid" id="F7B0J8"/>
    <dbReference type="OMA" id="VWEDMRA"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 4"/>
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    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0036157">
        <property type="term" value="C:outer dynein arm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0045504">
        <property type="term" value="F:dynein heavy chain binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0045503">
        <property type="term" value="F:dynein light chain binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="P:cilium movement"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="P:outer dynein arm assembly"/>
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        <property type="project" value="GO_Central"/>
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        <property type="term" value="P:regionalization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="entry name" value="Dynein axonemal intermediate chain 1"/>
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        <property type="entry name" value="Dynein axonemal intermediate chain 1"/>
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        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
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        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
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        <property type="entry name" value="WD40_repeat_dom_sf"/>
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        <property type="entry name" value="WD40_rpt"/>
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        <property type="entry name" value="DYNEIN AXONEMAL INTERMEDIATE CHAIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DYNEIN INTERMEDIATE CHAIN"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="WD_REPEATS_2"/>
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        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
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    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8" id="KW-0505">Motor protein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 15" id="KW-0853">WD repeat</keyword>
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        <location>
            <begin position="495"/>
            <end position="528"/>
        </location>
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            <begin position="95"/>
            <end position="183"/>
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    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="121"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
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            <begin position="149"/>
            <end position="164"/>
        </location>
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        <source>
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        </source>
    </evidence>
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        <source>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>F7B875</accession>
    <accession>A0A1W2VN06</accession>
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            <fullName evidence="10">Kinesin-like protein</fullName>
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        <name evidence="13" type="primary">kif3b</name>
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        <name type="scientific">Ciona intestinalis</name>
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    <reference evidence="14" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
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                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
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                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">wild type</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9 10">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01000249">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001007567.1">
        <property type="nucleotide sequence ID" value="NM_001007566.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F7B875"/>
    <dbReference type="FunCoup" id="F7B875">
        <property type="interactions" value="423"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000015247"/>
    <dbReference type="Ensembl" id="ENSCINT00000015247.3">
        <property type="protein sequence ID" value="ENSCINP00000015247.3"/>
        <property type="gene ID" value="ENSCING00000007410.3"/>
    </dbReference>
    <dbReference type="GeneID" id="493586"/>
    <dbReference type="KEGG" id="cin:493586"/>
    <dbReference type="CTD" id="9371"/>
    <dbReference type="GeneTree" id="ENSGT00940000153739"/>
    <dbReference type="HOGENOM" id="CLU_001485_22_4_1"/>
    <dbReference type="InParanoid" id="F7B875"/>
    <dbReference type="OMA" id="LESKMLC"/>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 1"/>
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    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0005871">
        <property type="term" value="C:kinesin complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="C:microtubule"/>
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        <property type="project" value="GO_Central"/>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="F:ATP hydrolysis activity"/>
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        <property type="term" value="F:microtubule binding"/>
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    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="CDD" id="cd01371">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000017">
        <property type="entry name" value="Kinesin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969">
        <property type="entry name" value="CHROMOSOME-ASSOCIATED KINESIN KIF4A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969:SF21">
        <property type="entry name" value="KINESIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
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    <keyword evidence="3 10" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7 9 10" id="KW-0505">Motor protein</keyword>
    <keyword evidence="4 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="9"/>
            <end position="338"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="11">
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            <end position="434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="698"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="372"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="388"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="411"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="704"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="95"/>
            <end position="102"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
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    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU000394"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000015247.3"/>
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    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>F7BHZ3</accession>
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        <recommendedName>
            <fullName evidence="2">Alpha-(1,6)-fucosyltransferase N- and catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01001172">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F7BHZ3"/>
    <dbReference type="STRING" id="7719.ENSCINP00000026387"/>
    <dbReference type="Ensembl" id="ENSCINT00000026633.2">
        <property type="protein sequence ID" value="ENSCINP00000026387.2"/>
        <property type="gene ID" value="ENSCING00000014657.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063737"/>
    <dbReference type="HOGENOM" id="CLU_1357348_0_0_1"/>
    <dbReference type="InParanoid" id="F7BHZ3"/>
    <dbReference type="OMA" id="WHATEFE"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046921">
        <property type="term" value="F:alpha-(1-&gt;6)-fucosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045573">
        <property type="entry name" value="Fut8_N_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13132">
        <property type="entry name" value="ALPHA- 1,6 -FUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13132:SF29">
        <property type="entry name" value="ALPHA-(1,6)-FUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19745">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003354148" description="Alpha-(1,6)-fucosyltransferase N- and catalytic domain-containing protein" evidence="1">
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            <begin position="28"/>
            <end position="230"/>
        </location>
    </feature>
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            <begin position="47"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000026387.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="230" mass="26605" checksum="725466CB68E78A7B" modified="2012-04-18" version="2">MRRNAKVFAFVASSVLMIGCLINKTFPKQNEFCFKQKTIHEMKAIVSVERPKATDIKRAKYAREIKAELQNLWHATEFEFKRVLRSNDRNKSIDQAEKDLLQTIWALEIQLENIQSLDITWQAVVANKTANMVSDLIDSHQNPHNCNERGQLFGHPEVCGYGCMVHNILEQFFMALSNNKTLFTYPFGCIYLGNETTLKNVFQPFSKNCSLRTGDWKEVETYTDLKVNDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>H2XJN7</accession>
    <name>H2XJN7_CIOIN</name>
    <protein>
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            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="41" mass="4630" checksum="BAEB0796B0F9F57F" modified="2012-04-18" version="1">IVCKLKQLNTIYKVLWGGILLAPNQIFSEQVLTINNTLLES</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>H2XLJ4</accession>
    <accession>A0A1W2W0X7</accession>
    <name>H2XLJ4_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">D-arabinitol dehydrogenase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100181990</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002120707.1">
        <property type="nucleotide sequence ID" value="XM_002120671.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XLJ4"/>
    <dbReference type="FunCoup" id="H2XLJ4">
        <property type="interactions" value="4"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000030526"/>
    <dbReference type="Ensembl" id="ENSCINT00000034468.1">
        <property type="protein sequence ID" value="ENSCINP00000030526.1"/>
        <property type="gene ID" value="ENSCING00000021058.1"/>
    </dbReference>
    <dbReference type="GeneID" id="100181990"/>
    <dbReference type="KEGG" id="cin:100181990"/>
    <dbReference type="GeneTree" id="ENSGT00940000170869"/>
    <dbReference type="HOGENOM" id="CLU_026673_11_0_1"/>
    <dbReference type="InParanoid" id="H2XLJ4"/>
    <dbReference type="OMA" id="MGLMMLE"/>
    <dbReference type="OrthoDB" id="3941538at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000721">
        <property type="term" value="F:(R,R)-butanediol dehydrogenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034079">
        <property type="term" value="P:butanediol biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050129">
        <property type="entry name" value="Zn_alcohol_dh"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43401">
        <property type="entry name" value="L-THREONINE 3-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43401:SF2">
        <property type="entry name" value="L-THREONINE 3-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alcohol dehydrogenase-like N-terminal" evidence="6">
        <location>
            <begin position="27"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="Alcohol dehydrogenase-like C-terminal" evidence="5">
        <location>
            <begin position="180"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000030526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="352" mass="38956" checksum="D9C3A36E48792D24" modified="2012-04-18" version="1">MQAVWWLGEKSDLSFKDKMKIPEVLKENDVKIKVAYSGVCGSDFHIMKGEMGKFSGKQDGVILGHEFSGTVVSVGDAVKELKMGDLVCVNPNECCGACQWGRESNPHFCESNLCVGISMNGGWAEYCIAPIEKVYKLPDNVNLMQGALTEPYSCVLRGWRQLGDLPEKDARILVQGAGIIGCLFCTLLHHHGFTNVTVAEINEERRKLCADMKLGFDVVHPQEIDLKFKGKDTDYHGYYVVIDCTGNTKAVEKAFTLCRRGATICIFGCCPAGETMAVEPFQIYWKELKIVSCFTNPKCFEGALELLKVLADKGYLVYEKLGIKMFDLKQYENAFGSLKAGTASKAVFKVDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>H2XLP7</accession>
    <name>H2XLP7_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">VWFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="H2XLP7"/>
    <dbReference type="Ensembl" id="ENSCINT00000031409.1">
        <property type="protein sequence ID" value="ENSCINP00000030579.1"/>
        <property type="gene ID" value="ENSCING00000022834.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000167387"/>
    <dbReference type="HOGENOM" id="CLU_008905_4_1_1"/>
    <dbReference type="InParanoid" id="H2XLP7"/>
    <dbReference type="OMA" id="EYARTDC"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050525">
        <property type="entry name" value="ECM_Assembly_Org"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24020">
        <property type="entry name" value="COLLAGEN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24020:SF20">
        <property type="entry name" value="PH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00453">
        <property type="entry name" value="VWFADOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003577239" description="VWFA domain-containing protein" evidence="2">
        <location>
            <begin position="20"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="3">
        <location>
            <begin position="49"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000030579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="209" mass="22828" checksum="92C5175D6073D86F" modified="2012-04-18" version="1">MMSLKLIFAVLAIAVFVNSQEVSPPPPPAPTTQPPPPPSATTECDNPIDLAFIVDSSSSVGADNFQLIKDFVYEIINGLNISETETRVTLARYNNWVDSRFFFNTHFDKSAMLDAVSNMPYYGRGTKTAQALHVVTSHHLQKYNGWREGVDAYPTVVIVLTDGRTKDFQDLGRFSRALKAKATRVIAIGVGDGAYADELVTIATAPSSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>H2XM67</accession>
    <name>H2XM67_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
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                <person name="Satou Y."/>
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                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
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                <person name="Huang W."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
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                <person name="Lindquist E."/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
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                <person name="Miura S."/>
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                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <consortium name="Ensembl"/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
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                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
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                <person name="Wang H.G."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Ueno K."/>
                <person name="Yamada L."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
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                <person name="Chin-Bow S."/>
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                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="5" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="5">wild type</strain>
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    <reference evidence="5" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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    <reference evidence="5" key="4">
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            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <property type="project" value="GO_Central"/>
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        <property type="entry name" value="PROTEIN STPG4"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR35678:SF1">
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Terry A."/>
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                <person name="Wang H.G."/>
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    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
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                <person name="Wiley G.B."/>
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                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
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                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">wild type</strain>
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    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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                <person name="Chapman J."/>
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                <person name="De Tomaso A."/>
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                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
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                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
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                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
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                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
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                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
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            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">wild type</strain>
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    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <source>
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        <source>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
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                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
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                <person name="Doggett N."/>
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                <person name="Richardson P."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
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                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
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                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
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                <person name="Lemaire P."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1">wild type</strain>
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    <reference evidence="1" key="3">
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <source>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Mineta K."/>
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                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
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                <person name="Wiley G.B."/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Chapman J."/>
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                <person name="De Tomaso A."/>
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                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Ueno K."/>
                <person name="Yamada L."/>
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                <consortium name="Ensembl"/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
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                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
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                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Putnam N."/>
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                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
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                <person name="Hayashi H."/>
                <person name="Hino K."/>
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                <person name="Goodstein D.M."/>
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                <person name="Lemaire P."/>
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                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
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                <person name="Putnam N."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
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        <property type="entry name" value="Lectin_C"/>
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        <source>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    <accession>H2Y0X5</accession>
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    <reference evidence="17" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
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                <person name="Putnam N."/>
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                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
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                <person name="Rigoutsos I."/>
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                <person name="Sasaki A."/>
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                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
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                <person name="Richardson P."/>
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                <person name="Kohara Y."/>
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    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
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        <reaction evidence="12">
            <text>3-fumarylpyruvate + H2O = fumarate + pyruvate + H(+)</text>
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            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16854"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a 3-acylpyruvate + H2O = a carboxylate + pyruvate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19009"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29067"/>
            <dbReference type="ChEBI" id="CHEBI:57278"/>
            <dbReference type="EC" id="3.7.1.5"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>acetylpyruvate + H2O = acetate + pyruvate + H(+)</text>
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            <dbReference type="ChEBI" id="CHEBI:15360"/>
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            <dbReference type="ChEBI" id="CHEBI:30089"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>oxaloacetate + H(+) = pyruvate + CO2</text>
            <dbReference type="Rhea" id="RHEA:15641"/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
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                <person name="Haga S."/>
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                <person name="Inaba K."/>
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                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
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                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Zhang C."/>
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                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2006" name="Dev. Biol." volume="292" first="546" last="554">
            <title>Systematic analysis of embryonic expression profiles of zinc finger genes in Ciona intestinalis.</title>
            <authorList>
                <person name="Miwata K."/>
                <person name="Chiba T."/>
                <person name="Horii R."/>
                <person name="Yamada L."/>
                <person name="Kubo A."/>
                <person name="Miyamura D."/>
                <person name="Satoh N."/>
                <person name="Satou Y."/>
            </authorList>
            <dbReference type="PubMed" id="16519883"/>
            <dbReference type="DOI" id="10.1016/j.ydbio.2006.01.024"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">wild type</strain>
        </source>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the unkempt family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001918">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="BR000190">
        <property type="protein sequence ID" value="FAA00221.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001123328.1">
        <property type="nucleotide sequence ID" value="NM_001129856.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1RL44"/>
    <dbReference type="STRING" id="7719.ENSCINP00000016180"/>
    <dbReference type="Ensembl" id="ENSCINT00000016180.3">
        <property type="protein sequence ID" value="ENSCINP00000016180.3"/>
        <property type="gene ID" value="ENSCING00000007900.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100169985"/>
    <dbReference type="KEGG" id="cin:100169985"/>
    <dbReference type="CTD" id="100169985"/>
    <dbReference type="GeneTree" id="ENSGT00940000169050"/>
    <dbReference type="HOGENOM" id="CLU_014526_1_0_1"/>
    <dbReference type="OMA" id="EWGCRDL"/>
    <dbReference type="OrthoDB" id="20534at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="Gene3D" id="3.30.1370.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040594">
        <property type="entry name" value="UNK_Znf_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057295">
        <property type="entry name" value="UNK_Znf_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057296">
        <property type="entry name" value="UNK_Znf_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045234">
        <property type="entry name" value="Unkempt-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14493:SF50">
        <property type="entry name" value="RING FINGER PROTEIN UNKEMPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14493">
        <property type="entry name" value="UNKEMPT FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23261">
        <property type="entry name" value="zf-CCCH_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25427">
        <property type="entry name" value="zf-CCCH_UNK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23035">
        <property type="entry name" value="zf-CCCH_UNK-like_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18384">
        <property type="entry name" value="zf_CCCH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="11">
        <location>
            <begin position="64"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="11">
        <location>
            <begin position="104"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="11">
        <location>
            <begin position="223"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="11">
        <location>
            <begin position="265"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="8">
        <location>
            <begin position="64"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="8">
        <location>
            <begin position="104"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="8">
        <location>
            <begin position="223"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="8">
        <location>
            <begin position="265"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="367"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="625"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="377"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="FAA00221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000016180.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="722" mass="81807" checksum="20AA6F0ACE830B87" modified="2006-05-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-16" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q1RL92</accession>
    <accession>A0A1W2VNR4</accession>
    <accession>F6U9E7</accession>
    <name>Q1RL92_CIOIN</name>
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        <submittedName>
            <fullName evidence="15 16">Zinc finger protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">Ci-ZF(C2H2)-130</name>
        <name evidence="16" type="synonym">zf(c2h2)-130</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2006" name="Dev. Biol." volume="292" first="546" last="554">
            <title>Systematic analysis of embryonic expression profiles of zinc finger genes in Ciona intestinalis.</title>
            <authorList>
                <person name="Miwata K."/>
                <person name="Chiba T."/>
                <person name="Horii R."/>
                <person name="Yamada L."/>
                <person name="Kubo A."/>
                <person name="Miyamura D."/>
                <person name="Satoh N."/>
                <person name="Satou Y."/>
            </authorList>
            <dbReference type="PubMed" id="16519883"/>
            <dbReference type="DOI" id="10.1016/j.ydbio.2006.01.024"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">wild type</strain>
        </source>
    </reference>
    <reference evidence="16" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the teashirt C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001754">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="BR000142">
        <property type="protein sequence ID" value="FAA00173.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001041462.1">
        <property type="nucleotide sequence ID" value="NM_001047997.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1RL92"/>
    <dbReference type="STRING" id="7719.ENSCINP00000007441"/>
    <dbReference type="Ensembl" id="ENSCINT00000007441.3">
        <property type="protein sequence ID" value="ENSCINP00000007441.3"/>
        <property type="gene ID" value="ENSCING00000003615.3"/>
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    <dbReference type="GeneID" id="751570"/>
    <dbReference type="KEGG" id="cin:751570"/>
    <dbReference type="CTD" id="751570"/>
    <dbReference type="GeneTree" id="ENSGT00950000183051"/>
    <dbReference type="HOGENOM" id="CLU_284816_0_0_1"/>
    <dbReference type="OrthoDB" id="5815793at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027008">
        <property type="entry name" value="Teashirt_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12487:SF7">
        <property type="entry name" value="PROTEIN TEASHIRT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12487">
        <property type="entry name" value="TEASHIRT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="262"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="317"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="473"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="107"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="357"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="512"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="596"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="769"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="812"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1020"/>
            <end position="1089"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="117"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="127"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="161"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="518"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="539"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="604"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="632"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
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            <begin position="769"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
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            <begin position="826"/>
            <end position="842"/>
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            <begin position="879"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1029"/>
            <end position="1041"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="FAA00173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000007441.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="1089" mass="120480" checksum="889ABC8C704A2964" modified="2006-05-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-02-07" modified="2026-01-28" version="112" xmlns="https://uniprot.org/uniprot">
    <accession>Q2PEE1</accession>
    <accession>A0A1W2VNS8</accession>
    <name>Q2PEE1_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Dynein light chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">Ci-LC6</name>
        <name evidence="10" type="synonym">Ci-LC8</name>
        <name evidence="11" type="synonym">dynll6</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2006-01" db="EMBL/GenBank/DDBJ databases">
            <title>Molecular characterization of outer arm dynein light chains.</title>
            <authorList>
                <person name="Inaba K."/>
                <person name="Hozumi A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Testis</tissue>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="journal article" date="2009" name="FEBS Lett." volume="583" first="2201" last="2207">
            <title>Proteomic characterization of sperm radial spokes identifies a novel spoke protein with an ubiquitin domain.</title>
            <authorList>
                <person name="Satouh Y."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="19527718"/>
            <dbReference type="DOI" id="10.1016/j.febslet.2009.06.016"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Testis</tissue>
        </source>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cytoplasm</location>
            <location evidence="1 8">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the dynein light chain family.</text>
    </comment>
    <dbReference type="EMBL" id="AB245513">
        <property type="protein sequence ID" value="BAE71140.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AB501297">
        <property type="protein sequence ID" value="BAH59282.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001037826.1">
        <property type="nucleotide sequence ID" value="NM_001044361.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2PEE1"/>
    <dbReference type="IntAct" id="Q2PEE1">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="MINT" id="Q2PEE1"/>
    <dbReference type="STRING" id="7719.ENSCINP00000035007"/>
    <dbReference type="Ensembl" id="ENSCINT00000030407.1">
        <property type="protein sequence ID" value="ENSCINP00000035007.1"/>
        <property type="gene ID" value="ENSCING00000023149.1"/>
    </dbReference>
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    <dbReference type="KEGG" id="cin:723807"/>
    <dbReference type="CTD" id="723807"/>
    <dbReference type="GeneTree" id="ENSGT00390000000378"/>
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    <dbReference type="OMA" id="THEKGHF"/>
    <dbReference type="OrthoDB" id="10033309at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005868">
        <property type="term" value="C:cytoplasmic dynein complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035721">
        <property type="term" value="P:intraciliary retrograde transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044458">
        <property type="term" value="P:motile cilium assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="DLC-like_DYNLL1_DYNLL2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Dynein light chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.740.10">
        <property type="entry name" value="Protein Inhibitor Of Neuronal Nitric Oxide Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037177">
        <property type="entry name" value="DLC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019763">
        <property type="entry name" value="Dynein_light_1/2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001372">
        <property type="entry name" value="Dynein_light_chain_typ-1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11886">
        <property type="entry name" value="DYNEIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11886:SF35">
        <property type="entry name" value="DYNEIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01221">
        <property type="entry name" value="Dynein_light"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Dynein_light"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="DLC"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="DYNEIN_LIGHT_1"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5 8" id="KW-0243">Dynein</keyword>
    <keyword evidence="4 8" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6 8" id="KW-0505">Motor protein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU365010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAE71140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAH59282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000035007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="89" mass="10306" checksum="93BD3D240DDC7BC7" modified="2006-02-07" version="1">MSDRKAVIKNADMAEDMQQDAVDCATQALEKYNIEKDIAAYIKKEFDKKYNPTWHCICGRNFGSYVTHETKHFIYFYLGQVAVLLFKSG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-03-24" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>Q4H3N8</accession>
    <name>ENY2_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Transcription and mRNA export factor ENY2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Enhancer of yellow 2 transcription factor homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">ENY2</name>
    </gene>
    <organism>
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Development" volume="131" first="4047" last="4058">
            <title>Gene expression profiles of transcription factors and signaling molecules in the ascidian embryo: towards a comprehensive understanding of gene networks.</title>
            <authorList>
                <person name="Imai K.S."/>
                <person name="Hino K."/>
                <person name="Yagi K."/>
                <person name="Satoh N."/>
                <person name="Satou Y."/>
            </authorList>
            <dbReference type="PubMed" id="15269171"/>
            <dbReference type="DOI" id="10.1242/dev.01270"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [MRNA]</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in mRNA export coupled transcription activation by association with both the TREX-2 and the SAGA complexes. The transcription regulatory histone acetylation (HAT) complex SAGA is a multiprotein complex that activates transcription by remodeling chromatin and mediating histone acetylation and deubiquitination. Within the SAGA complex, participates in a subcomplex that specifically deubiquitinates histones. The SAGA complex is recruited to specific gene promoters by activators, where it is required for transcription. The TREX-2 complex functions in docking export-competent ribonucleoprotein particles (mRNPs) to the nuclear entrance of the nuclear pore complex (nuclear basket). TREX-2 participates in mRNA export and accurate chromatin positioning in the nucleus by tethering genes to the nuclear periphery.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the nuclear pore complex (NPC)-associated TREX-2 complex (transcription and export complex 2). Component of the SAGA transcription coactivator-HAT complex. Within the SAGA complex, participates in a subcomplex of SAGA called the DUB module (deubiquitination module).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ENY2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AB210384">
        <property type="protein sequence ID" value="BAE06389.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001071686.1">
        <property type="nucleotide sequence ID" value="NM_001078218.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q4H3N8"/>
    <dbReference type="SMR" id="Q4H3N8"/>
    <dbReference type="FunCoup" id="Q4H3N8">
        <property type="interactions" value="585"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000005631"/>
    <dbReference type="Ensembl" id="ENSCINT00000005631.2">
        <property type="protein sequence ID" value="ENSCINP00000005631.2"/>
        <property type="gene ID" value="ENSCING00000002757.2"/>
    </dbReference>
    <dbReference type="GeneID" id="778583"/>
    <dbReference type="KEGG" id="cin:778583"/>
    <dbReference type="CTD" id="45848"/>
    <dbReference type="eggNOG" id="KOG4479">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000011748"/>
    <dbReference type="HOGENOM" id="CLU_134052_1_1_1"/>
    <dbReference type="InParanoid" id="Q4H3N8"/>
    <dbReference type="OMA" id="RLMCRNI"/>
    <dbReference type="OrthoDB" id="6221744at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071819">
        <property type="term" value="C:DUBm complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070390">
        <property type="term" value="C:transcription export complex 2"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.246.140:FF:000001">
        <property type="entry name" value="Transcription and mRNA export factor ENY2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03046">
        <property type="entry name" value="ENY2_Sus1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018783">
        <property type="entry name" value="TF_ENY2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038212">
        <property type="entry name" value="TF_EnY2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12514">
        <property type="entry name" value="ENHANCER OF YELLOW 2 TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10163">
        <property type="entry name" value="EnY2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0010">Activator</keyword>
    <keyword id="KW-0156">Chromatin regulator</keyword>
    <keyword id="KW-0509">mRNA transport</keyword>
    <keyword id="KW-0539">Nucleus</keyword>
    <keyword id="KW-0653">Protein transport</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <keyword id="KW-0811">Translocation</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000367550" description="Transcription and mRNA export factor ENY2">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="104" mass="12101" checksum="256B071C800835E8" modified="2005-08-30" version="1">MADYGSDKKSRDNQMRSAINQQLVETGEREKLKELLRVRLAECGWRDQLKQLCKEIVRERGLEHVSVDDLVQDITPKARQLVPDTVKKELLQKIRNFLAQQANV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-06-07" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1W2W0N1</accession>
    <accession>F6XY36</accession>
    <name>A0A1W2W0N1_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">3-oxo-5-alpha-steroid 4-dehydrogenase</fullName>
            <ecNumber evidence="18">1.3.1.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">LOC100186253</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14 18">Converts testosterone into 5-alpha-dihydrotestosterone and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>17beta-hydroxy-5alpha-androstan-3-one + NADP(+) = testosterone + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50820"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16330"/>
            <dbReference type="ChEBI" id="CHEBI:17347"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.3.1.22"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="17">
            <dbReference type="Rhea" id="RHEA:50822"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>5alpha-pregnane-3,20-dione + NADP(+) = progesterone + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21952"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17026"/>
            <dbReference type="ChEBI" id="CHEBI:28952"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.3.1.22"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="15">
            <dbReference type="Rhea" id="RHEA:21954"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>a 3-oxo-5alpha-steroid + NADP(+) = a 3-oxo-Delta(4)-steroid + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54384"/>
            <dbReference type="ChEBI" id="CHEBI:13601"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:47909"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.3.1.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>androst-4-ene-3,17-dione + NADPH + H(+) = 5alpha-androstan-3,17-dione + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:50816"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15994"/>
            <dbReference type="ChEBI" id="CHEBI:16422"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:50817"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Microsome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 18">Belongs to the steroid 5-alpha reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.22" evidence="18"/>
    <dbReference type="RefSeq" id="XP_002120345.1">
        <property type="nucleotide sequence ID" value="XM_002120309.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1W2W0N1"/>
    <dbReference type="Ensembl" id="ENSCINT00000027314.2">
        <property type="protein sequence ID" value="ENSCINP00000027068.2"/>
        <property type="gene ID" value="ENSCING00000015182.2"/>
    </dbReference>
    <dbReference type="GeneID" id="100186253"/>
    <dbReference type="KEGG" id="cin:100186253"/>
    <dbReference type="eggNOG" id="KOG1638">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182886"/>
    <dbReference type="InParanoid" id="A0A1W2W0N1"/>
    <dbReference type="OMA" id="PHYALEW"/>
    <dbReference type="OrthoDB" id="5788137at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047751">
        <property type="term" value="F:3-oxo-5-alpha-steroid 4-dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003865">
        <property type="term" value="F:3-oxo-5-alpha-steroid 4-dehydrogenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006702">
        <property type="term" value="P:androgen biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008584">
        <property type="term" value="P:male gonad development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1630:FF:000002">
        <property type="entry name" value="Steroid 5 alpha-reductase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016636">
        <property type="entry name" value="3-oxo-5-alpha-steroid_4-DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001104">
        <property type="entry name" value="3-oxo-5_a-steroid_4-DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039357">
        <property type="entry name" value="SRD5A/TECR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10556">
        <property type="entry name" value="3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10556:SF57">
        <property type="entry name" value="3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02544">
        <property type="entry name" value="Steroid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015596">
        <property type="entry name" value="5_alpha-SR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50244">
        <property type="entry name" value="S5A_REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0221">Differentiation</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0492">Microsome</keyword>
    <keyword evidence="8" id="KW-0521">NADP</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0726">Sexual differentiation</keyword>
    <keyword evidence="4 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="24"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="123"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="156"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="3-oxo-5-alpha-steroid 4-dehydrogenase C-terminal" evidence="19">
        <location>
            <begin position="120"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007742"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022928"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00037789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049166"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRNR" id="PIRNR015596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF02544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000027068.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="268" mass="30877" checksum="7591C7F6ED8FDF75" modified="2017-06-07" version="1">MSGYLRIFYNVLTGAEDLKKQIDLMAGVLIGVSPIIHLIIVSGINAPYGRYTSSSWGLPINSKLAWFLQELPAFLIPVFSYFTMTSSNVSTPALILLSVFLLHYFNRTFIYSQKIRGGKPTPILIFLMAMFFCICNGYMQSVFLLQISNYREEYLFEWNFVVGLILFFLGMYINLECDSILRNLRKEGEKGYKIPTGNMFNYVTGGNLFGESLEWCGWGLACWSIQGFAFATFAVMYLSARSYSHHVWYLKKFEDYPKDRKIFIPYIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-06-07" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1W5BJ73</accession>
    <accession>H2XKK9</accession>
    <name>A0A1W5BJ73_CIOIN</name>
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        <recommendedName>
            <fullName evidence="14">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">zf(c2h2)-118</name>
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    <organism evidence="15 16">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">wild type</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001318">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="RefSeq" id="XP_018670236.1">
        <property type="nucleotide sequence ID" value="XM_018814691.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1W5BJ73"/>
    <dbReference type="Ensembl" id="ENSCINT00000033325.1">
        <property type="protein sequence ID" value="ENSCINP00000030191.1"/>
        <property type="gene ID" value="ENSCING00000021732.1"/>
    </dbReference>
    <dbReference type="GeneID" id="778874"/>
    <dbReference type="CTD" id="778874"/>
    <dbReference type="GeneTree" id="ENSGT00940000162287"/>
    <dbReference type="InParanoid" id="A0A1W5BJ73"/>
    <dbReference type="OMA" id="MSHENKF"/>
    <dbReference type="OrthoDB" id="9439254at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032502">
        <property type="term" value="P:developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000045">
        <property type="entry name" value="ZFP69 zinc finger protein B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000193">
        <property type="entry name" value="Zinc finger protein 300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000690">
        <property type="entry name" value="Zinc finger protein 354C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000202">
        <property type="entry name" value="Zinc finger protein 574"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000624">
        <property type="entry name" value="zinc finger protein 697"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24376">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24376:SF216">
        <property type="entry name" value="ZINC FINGER PROTEIN 420-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="12"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="15"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="113"/>
            <end position="136"/>
        </location>
    </feature>
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        <location>
            <begin position="150"/>
            <end position="177"/>
        </location>
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            <begin position="178"/>
            <end position="205"/>
        </location>
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    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="205"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="236"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="264"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="291"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="393"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="423"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="451"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="479"/>
            <end position="501"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000030191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="505" mass="60229" checksum="D1D7646268B22C7A" modified="2017-06-07" version="1">MSRILHLTRSSEKVYECSKCGLMFKRMSSLKSHEKLVHGDGDFAVDAYCNVEPELKLNIEKENKKTVKNKRNKENIKTKKNERIVESKITVKRDKNLFRKPSKREAVSGKSLFECEYCDKAYTRKDHKTRHQKKVHGIMPTPQVKTKMIRKCNYCERTFTKLDQFRIHERTHTGERPYVCQHCSVAFMSRNNLTRHERRHGEKEFECDLCGKKYFEKSSLITHMKTHSKGKLDLKHPCTICDKRFNCNSRLVIHMRTHTGEKPFVCNVCDLAFIAKYRLQRHLQLHTDNLYECSVCNKTFNMFRYLQRHMAVHLKDKPFECHVCGKRYKSLKSKQEHVKLQHLLKILCTSCDKSYYKKFLYLNYETGQGYTCVYCIRKAAHKDTKDPKPRYEFFCEYEGCPKSFKYFQSLKQHLLIHTGKNLFECQRCDKKFAKKYHLKEHEWTHSGYRPYKCDQCPKTYSRNSHLNHHKLSHAGVKPHQCNECGRKFLRKQTLNSHKRTHIVIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>F6PJP0</accession>
    <name>F6PJP0_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">C-type lectin domain family 4 member C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC100181550</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="F6PJP0"/>
    <dbReference type="Ensembl" id="ENSCINT00000028974.2">
        <property type="protein sequence ID" value="ENSCINP00000028728.2"/>
        <property type="gene ID" value="ENSCING00000016662.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000068648"/>
    <dbReference type="HOGENOM" id="CLU_1513879_0_0_1"/>
    <dbReference type="InParanoid" id="F6PJP0"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00037">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003344080" evidence="1">
        <location>
            <begin position="18"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000028728.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="178" mass="20353" checksum="2E75D59D5B71D8C9" modified="2012-04-18" version="2">MLFISVMLLLLLNNAFAEKLKFQRGTTEDFSYSLTSSKAKLTVGLMTCFSSPPKNAAVTLVRPTDASLQHRFKAKVLQIFSDSLSIELERVDVHSSWEWIELKIEWIVYLENAGSDWFEASNGLLYKYIPIRMSYEKAKTECKKLGAWVVVHASTNETVLTQMHNELVPAIKLRYWVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>F6PQ66</accession>
    <name>F6PQ66_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">[histone H3]-lysine(4) N-methyltransferase</fullName>
            <ecNumber evidence="15">2.1.1.364</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">wild type</strain>
        </source>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-lysyl(4)-[histone H3] + S-adenosyl-L-methionine = N(6)-methyl-L-lysyl(4)-[histone H3] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:60264"/>
            <dbReference type="Rhea" id="RHEA-COMP:15543"/>
            <dbReference type="Rhea" id="RHEA-COMP:15547"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61929"/>
            <dbReference type="EC" id="2.1.1.364"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:60265"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.1.1.364" evidence="15"/>
    <dbReference type="EMBL" id="EAAA01000612">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000025384"/>
    <dbReference type="Ensembl" id="ENSCINT00000025630.2">
        <property type="protein sequence ID" value="ENSCINP00000025384.2"/>
        <property type="gene ID" value="ENSCING00000006467.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163756"/>
    <dbReference type="HOGENOM" id="CLU_000208_0_0_1"/>
    <dbReference type="InParanoid" id="F6PQ66"/>
    <dbReference type="OMA" id="EMIMEYT"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140945">
        <property type="term" value="F:histone H3K4 monomethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15664">
        <property type="entry name" value="ePHD_KMT2A_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19170">
        <property type="entry name" value="SET_KMT2A_2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.270.10:FF:000004">
        <property type="entry name" value="Histone-lysine N-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.360:FF:000015">
        <property type="entry name" value="Histone-lysine N-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000002">
        <property type="entry name" value="Histone-lysine N-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034732">
        <property type="entry name" value="EPHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003889">
        <property type="entry name" value="FYrich_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003888">
        <property type="entry name" value="FYrich_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047219">
        <property type="entry name" value="KMT2A_2B_SET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003616">
        <property type="entry name" value="Post-SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45838:SF4">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE TRITHORAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45838">
        <property type="entry name" value="HISTONE-LYSINE-N-METHYLTRANSFERASE 2 KMT2 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05965">
        <property type="entry name" value="FYRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05964">
        <property type="entry name" value="FYRN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13771">
        <property type="entry name" value="zf-HC5HC2H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00542">
        <property type="entry name" value="FYRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51805">
        <property type="entry name" value="EPHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51543">
        <property type="entry name" value="FYRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51542">
        <property type="entry name" value="FYRN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50868">
        <property type="entry name" value="POST_SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="9" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="13" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="20">
        <location>
            <begin position="135"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="SET" evidence="18">
        <location>
            <begin position="1175"/>
            <end position="1291"/>
        </location>
    </feature>
    <feature type="domain" description="Post-SET" evidence="19">
        <location>
            <begin position="1299"/>
            <end position="1315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="98"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="359"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="438"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="709"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="805"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="111"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="438"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="709"/>
            <end position="723"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000025384.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="1315" mass="147990" checksum="BAA34AE4C6215901" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>F6PQ74</accession>
    <name>F6PQ74_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cytoplasmic phosphatidylinositol transfer protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Retinal degeneration B homolog beta</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">wild type</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the transfer of phosphatidylinositol (PI) and phosphatidic acid (PA) between membranes. Binds PA derived from the phospholipase D signaling pathway and among the cellular PA species, preferably binds to the C16:0/16:1 and C16:1/18:1 PA species.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 1,2-diacyl-sn-glycero-3-phosphate(in) = a 1,2-diacyl-sn-glycero-3-phosphate(out)</text>
            <dbReference type="Rhea" id="RHEA:36435"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:36436"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol)(in) = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol)(out)</text>
            <dbReference type="Rhea" id="RHEA:38691"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="4">
            <dbReference type="Rhea" id="RHEA:38692"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the PtdIns transfer protein family. PI transfer class IIB subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01000612">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6PQ74"/>
    <dbReference type="STRING" id="7719.ENSCINP00000025383"/>
    <dbReference type="Ensembl" id="ENSCINT00000025629.2">
        <property type="protein sequence ID" value="ENSCINP00000025383.2"/>
        <property type="gene ID" value="ENSCING00000013931.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163128"/>
    <dbReference type="HOGENOM" id="CLU_046509_2_0_1"/>
    <dbReference type="InParanoid" id="F6PQ74"/>
    <dbReference type="OMA" id="VENRPCE"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008526">
        <property type="term" value="F:phosphatidylinositol transfer activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd08890">
        <property type="entry name" value="SRPBCC_PITPNC1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.530.20:FF:000011">
        <property type="entry name" value="cytoplasmic phosphatidylinositol transfer protein 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001666">
        <property type="entry name" value="PI_transfer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055261">
        <property type="entry name" value="PI_transfer_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10658:SF54">
        <property type="entry name" value="CYTOPLASMIC PHOSPHATIDYLINOSITOL TRANSFER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10658">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL TRANSFER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02121">
        <property type="entry name" value="IP_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00391">
        <property type="entry name" value="PITRANSFER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="3" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Phosphatidylinositol transfer protein N-terminal" evidence="11">
        <location>
            <begin position="2"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="248"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="256"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="272"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00051611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00055461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00061154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00068698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00082927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000025383.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="283" mass="33298" checksum="4FF3DDF4FB03CD3E" modified="2012-04-18" version="2">AMLLKEYRICMPLSVEEYQRGQLYMINRHSNEQSESGEGVEVIKNEPVYTEENGDGQFTEKRIHLNNRLPNWARSFVPSIYVTEKAWNYYPYTITEYTCPWLPRLHIHIETRYENNNGHNNDVSKEIDPKEFTKREVIHLDLAYDKVPDKHYKADEDCTKFKSIKTGRGPLKPNWRESHSPIMCSYKLVQVKFEVWGMQTKVESYIHKVVRDVLLLAHRQAFAWIDTWYDMTFDNIRRYESEIQEKTNAKVKSETSHFPSTPTSPTIPSVQLPGTSKTKAWGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>F6PU57</accession>
    <accession>A0A1W2WL08</accession>
    <name>F6PU57_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized LOC100181536</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC100181536</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18026">
        <property type="entry name" value="DEXHc_POLQ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18795">
        <property type="entry name" value="SF2_C_Ski2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3380.20:FF:000002">
        <property type="entry name" value="helicase POLQ-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000813">
        <property type="entry name" value="helicase POLQ-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3380.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050474">
        <property type="entry name" value="Hel308_SKI2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046931">
        <property type="entry name" value="HTH_61"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048960">
        <property type="entry name" value="POLQ-like_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961">
        <property type="entry name" value="DNA POLYMERASE THETA, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G05260)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961:SF12">
        <property type="entry name" value="HELICASE POLQ-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20470">
        <property type="entry name" value="HTH_61"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21099">
        <property type="entry name" value="POLQ_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158702">
        <property type="entry name" value="Sec63 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="11">
        <location>
            <begin position="71"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="12">
        <location>
            <begin position="294"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="11"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000013050.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="804" mass="89869" checksum="A0C9CECCBE81D18B" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>F6Q1G7</accession>
    <name>F6Q1G7_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Outer mitochondrial transmembrane helix translocase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>[protein]-with a C-terminal TM segment(out) + ATP + H2O = [protein]-with a C-terminal TM segment(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66168"/>
            <dbReference type="Rhea" id="RHEA-COMP:16963"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:90782"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Peroxisome membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the AAA ATPase family. MSP1 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="F6Q1G7"/>
    <dbReference type="FunCoup" id="F6Q1G7">
        <property type="interactions" value="214"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000001368"/>
    <dbReference type="Ensembl" id="ENSCINT00000001368.3">
        <property type="protein sequence ID" value="ENSCINP00000001368.3"/>
        <property type="gene ID" value="ENSCING00000000743.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166329"/>
    <dbReference type="HOGENOM" id="CLU_000688_21_14_1"/>
    <dbReference type="InParanoid" id="F6Q1G7"/>
    <dbReference type="OMA" id="NTRQRQD"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140570">
        <property type="term" value="P:extraction of mislocalized protein from mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000538">
        <property type="entry name" value="ATPase family AAA domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051701">
        <property type="entry name" value="Mito_OM_Translocase_MSP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45644">
        <property type="entry name" value="AAA ATPASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G12920)-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45644:SF3">
        <property type="entry name" value="FI08533P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="10">
        <location>
            <begin position="136"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000001368.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="283" mass="31723" checksum="C9B93156D044D370" modified="2012-04-18" version="2">MGVKLEVRGFEDAEVTSKSQLIGLIVRLVFTGAAGYFLYKYVDKVLGILDPTKKQKKASEEKAQALLKLLGKHNITDLNLTEYELNIASQLIIPKDIPVSWNQIGGLDYIVEQIKETVILPFHKRDIFRQCKLFLPPKGILLYGPPGCGKTMIAKATAREAGCAFINIEVQQLTDKWYGESQKLAAAVFSLAHKLQPAIIFIDEIDAFLQMRSDRDHEVTAMMKATFMSLWDGLASDNESQVMVMGATNRPQQIDQAILRRMPIKLNVPMPLWHSFEAATTTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>F6Q5B7</accession>
    <accession>A0A1W2WCK3</accession>
    <name>F6Q5B7_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Plasminogen receptor (KT)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">LOC100185164</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_002128610.1">
        <property type="nucleotide sequence ID" value="XM_002128574.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6Q5B7"/>
    <dbReference type="FunCoup" id="F6Q5B7">
        <property type="interactions" value="4"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000001320"/>
    <dbReference type="Ensembl" id="ENSCINT00000001320.3">
        <property type="protein sequence ID" value="ENSCINP00000001320.3"/>
        <property type="gene ID" value="ENSCING00000016124.2"/>
    </dbReference>
    <dbReference type="GeneID" id="100185164"/>
    <dbReference type="KEGG" id="cin:100185164"/>
    <dbReference type="GeneTree" id="ENSGT00390000000375"/>
    <dbReference type="HOGENOM" id="CLU_115107_1_0_1"/>
    <dbReference type="InParanoid" id="F6Q5B7"/>
    <dbReference type="OMA" id="MGYYTDW"/>
    <dbReference type="OrthoDB" id="9983019at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010756">
        <property type="term" value="P:positive regulation of plasminogen activation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019319">
        <property type="entry name" value="Plg-R(KT)"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13411">
        <property type="entry name" value="PLASMINOGEN RECEPTOR (KT)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13411:SF6">
        <property type="entry name" value="PLASMINOGEN RECEPTOR (KT)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10166">
        <property type="entry name" value="DUF2368"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000001320.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="152" mass="17178" checksum="40C40120DEA80D31" modified="2011-07-27" version="1">MGQISSSLSSGMKGVMEENMKKQMDFQMGAFQLQMERQLAMQNEMRERQMSMGIARAREVVKYYGSFYAVLAVLGPVAAYKTKSATPVIPLIPLGFVLTYQLDAAYGTLLSRVRSEAEHIMNVERDMISLPKGMPTFEIIESVRLENKKKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>F6RGI4</accession>
    <accession>A0A1W2WI70</accession>
    <name>F6RGI4_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL11</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">60S ribosomal protein L12</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100184231</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">wild type</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the large ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. Binds directly to 26S ribosomal RNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains about 33 different proteins and 1 molecule of RNA (18S). The 60S subunit contains about 49 different proteins and 3 molecules of RNA (28S, 5.8S and 5S).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the universal ribosomal protein uL11 family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002974">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002131561.1">
        <property type="nucleotide sequence ID" value="XM_002131525.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6RGI4"/>
    <dbReference type="FunCoup" id="F6RGI4">
        <property type="interactions" value="442"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000023959"/>
    <dbReference type="Ensembl" id="ENSCINT00000024205.2">
        <property type="protein sequence ID" value="ENSCINP00000023959.2"/>
        <property type="gene ID" value="ENSCING00000012950.2"/>
    </dbReference>
    <dbReference type="GeneID" id="100184231"/>
    <dbReference type="KEGG" id="cin:100184231"/>
    <dbReference type="GeneTree" id="ENSGT00390000006922"/>
    <dbReference type="HOGENOM" id="CLU_074237_5_0_1"/>
    <dbReference type="InParanoid" id="F6RGI4"/>
    <dbReference type="OMA" id="QPPHDVI"/>
    <dbReference type="OrthoDB" id="1478556at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.250:FF:000002">
        <property type="entry name" value="60S ribosomal protein L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1550.10:FF:000002">
        <property type="entry name" value="60S ribosomal protein L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.250">
        <property type="entry name" value="Ribosomal protein L11, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1550.10">
        <property type="entry name" value="Ribosomal protein L11/L12, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00736">
        <property type="entry name" value="Ribosomal_uL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000911">
        <property type="entry name" value="Ribosomal_uL11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020783">
        <property type="entry name" value="Ribosomal_uL11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036769">
        <property type="entry name" value="Ribosomal_uL11_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020785">
        <property type="entry name" value="Ribosomal_uL11_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020784">
        <property type="entry name" value="Ribosomal_uL11_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036796">
        <property type="entry name" value="Ribosomal_uL11_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11661">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11661:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00298">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03946">
        <property type="entry name" value="Ribosomal_L11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00649">
        <property type="entry name" value="RL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54747">
        <property type="entry name" value="Ribosomal L11/L12e N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46906">
        <property type="entry name" value="Ribosomal protein L11, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00359">
        <property type="entry name" value="RIBOSOMAL_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 8" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL11 N-terminal" evidence="10">
        <location>
            <begin position="13"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL11 C-terminal" evidence="9">
        <location>
            <begin position="74"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00045484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00046571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000023959.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="165" mass="17870" checksum="6446AE692162E9AC" modified="2012-04-18" version="2">MPPKFDPTAIHYVYLRVTGGEVGATASLAPKIGPLGLSPKKVGDDIAKSTQDWKGLKITVCLKIQNRQATVSVVPSAASLVIKALKEPPRDKKKVKNIKHNGNITMEDVYGVARIMRERSMSRFFSGTVKEILGTAQSVGCTVDGKPPHDVIDMINDGEIECPDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>F6RHG2</accession>
    <accession>A0A1W2WJV7</accession>
    <name>F6RHG2_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 13">Vacuolar protein sorting-associated protein 29</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12 13">Vesicle protein sorting 29</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100176748</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">wild type</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Component of the commander complex that is essential for endosomal recycling of transmembrane cargos; the commander complex is composed of the Csubcomplex and the retriever subcomplex. Component of the retriever complex, which is a heterotrimeric complex related to retromer cargo-selective complex (CSC) and essential for retromer-independent retrieval and recycling of numerous cargos. Component of the retromer cargo-selective complex (CSC). The CSC is believed to be the core functional component of retromer or respective retromer complex variants acting to prevent missorting of selected transmembrane cargo proteins into the lysosomal degradation pathway. In the endosomes, retriever complex drives the retrieval and recycling of NxxY-motif-containing cargo proteins by coupling to snx17, a cargo essential for the homeostatic maintenance of numerous cell surface proteins associated with processes that include cell migration, cell adhesion, nutrient supply and cell signaling. The recruitment of the retriever complex to the endosomal membrane involves Cand WASH complexes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the VPS29 family.</text>
    </comment>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="RefSeq" id="XP_002130329.1">
        <property type="nucleotide sequence ID" value="XM_002130293.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6RHG2"/>
    <dbReference type="FunCoup" id="F6RHG2">
        <property type="interactions" value="842"/>
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    <dbReference type="STRING" id="7719.ENSCINP00000009651"/>
    <dbReference type="Ensembl" id="ENSCINT00000009651.3">
        <property type="protein sequence ID" value="ENSCINP00000009651.3"/>
        <property type="gene ID" value="ENSCING00000004662.3"/>
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    <dbReference type="KEGG" id="cin:100176748"/>
    <dbReference type="GeneTree" id="ENSGT00390000012669"/>
    <dbReference type="HOGENOM" id="CLU_063749_0_1_1"/>
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        <property type="component" value="Chromosome 10"/>
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        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
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        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
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        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0030904">
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        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0046872">
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
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    </dbReference>
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        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
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        <property type="entry name" value="MPP_Vps29"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Vacuolar protein sorting-associated protein 29"/>
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        <property type="entry name" value="Calcineurin-like_PHP_lpxH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000979">
        <property type="entry name" value="Phosphodiesterase_MJ0936/Vps29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028661">
        <property type="entry name" value="Vps29"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00040">
        <property type="entry name" value="yfcE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11124">
        <property type="entry name" value="VACUOLAR SORTING PROTEIN VPS29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12850">
        <property type="entry name" value="Metallophos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0967">Endosome</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 13" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0813">Transport</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="14">
        <location>
            <begin position="4"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU362040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF12850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000009651.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="183" mass="20645" checksum="B91AA04CF02282E4" modified="2011-07-27" version="1">MVLVLVVGDMHIPFRASGLSPKFKKLLVPGKIQHILCTGNLCTRESYDYLKTLASDVHVVRGDFDENVNYPEQKVVTVGQFRIGMCHGHQICPWGDTESLAMLQRQLNVDILIFGHTHKFEAYEHESHFYINPGSITGAFSPTASEVIPSFVLMDIQASTVVTYVYQLQANDVKVERIEYQKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>F6RKY2</accession>
    <name>F6RKY2_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">2'-phosphotransferase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
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            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">wild type</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the KptA/TPT1 family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01000103">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="Ensembl" id="ENSCINT00000025368.2">
        <property type="protein sequence ID" value="ENSCINP00000025122.2"/>
        <property type="gene ID" value="ENSCING00000002405.3"/>
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    <dbReference type="GeneTree" id="ENSGT00390000002731"/>
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    <dbReference type="InParanoid" id="F6RKY2"/>
    <dbReference type="OMA" id="IREDIGW"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000215">
        <property type="term" value="F:tRNA 2'-phosphotransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006388">
        <property type="term" value="P:tRNA splicing, via endonucleolytic cleavage and ligation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Ptrans_KptA/Tpt1"/>
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    </evidence>
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        <source>
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    </evidence>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">wild type</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the TGF-beta family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="ENSCING00000006362.3"/>
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    <dbReference type="HOGENOM" id="CLU_410455_0_0_1"/>
    <dbReference type="InParanoid" id="F6RS38"/>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:cell surface receptor protein serine/threonine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="entry name" value="Cystine-knot cytokines"/>
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        <property type="entry name" value="TGF-beta-rel"/>
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        <property type="entry name" value="BONE MORPHOGENETIC PROTEIN 3-LIKE"/>
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    <dbReference type="PANTHER" id="PTHR11848">
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>F6RWZ5</accession>
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        <recommendedName>
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    <organism evidence="4 5">
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    <reference evidence="5" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Matsumoto J."/>
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                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">wild type</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">GTPase that recruits MYO1E to MHC class II-containing vesicles via the effector protein ARL14EP and hence controls the movement of these vesicles along the actin cytoskeleton in dendritic cells.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Interacts with ARL14EP.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01000475">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6S959"/>
    <dbReference type="STRING" id="7719.ENSCINP00000026636"/>
    <dbReference type="Ensembl" id="ENSCINT00000026882.2">
        <property type="protein sequence ID" value="ENSCINP00000026636.2"/>
        <property type="gene ID" value="ENSCING00000014827.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165938"/>
    <dbReference type="HOGENOM" id="CLU_040729_9_2_1"/>
    <dbReference type="InParanoid" id="F6S959"/>
    <dbReference type="OMA" id="HYYQNSE"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030010">
        <property type="term" value="P:establishment of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00878">
        <property type="entry name" value="Arf_Arl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000412">
        <property type="entry name" value="ADP-ribosylation factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024156">
        <property type="entry name" value="Small_GTPase_ARF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11711">
        <property type="entry name" value="ADP RIBOSYLATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00328">
        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8 10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 8 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="9">
        <location>
            <position position="6"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="102"/>
            <end position="105"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00054077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00061881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00072405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00077764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000026636.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="163" mass="18599" checksum="BC1EB306AF93452B" modified="2012-04-18" version="2">DFEGKTTITYKLALNEVVQTIPTIEFNVETVSPCKGLSLTIWDVGGQEILRKLWQHYYNNTDGFIYVVDSTDQVRFSEAKEELHNALEHDALRRVPVLVFANKQDMPYAVPVSKLADALELNKLDRDHKWHIQSCCAVTGDGLMEGFEVFASMMKEYKQVNAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>F6SMG7</accession>
    <name>F6SMG7_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ZP-C domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01001616">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6SMG7"/>
    <dbReference type="Ensembl" id="ENSCINT00000022608.2">
        <property type="protein sequence ID" value="ENSCINP00000022362.2"/>
        <property type="gene ID" value="ENSCING00000011784.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000064717"/>
    <dbReference type="HOGENOM" id="CLU_1277232_0_0_1"/>
    <dbReference type="InParanoid" id="F6SMG7"/>
    <dbReference type="OMA" id="MCKMLSQ"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.4100">
        <property type="entry name" value="Zona pellucida, ZP-C domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055355">
        <property type="entry name" value="ZP-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042235">
        <property type="entry name" value="ZP-C_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00100">
        <property type="entry name" value="Zona_pellucida"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="173"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="ZP-C" evidence="2">
        <location>
            <begin position="18"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000022362.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="216" mass="23619" checksum="DC1FB89EE85AD6D4" modified="2012-04-18" version="2">MCKMLSQCPAACPPSYIVQPNGVYTVGEDLNIVIQHNREIVPTGSTLSVQTLYISCSSDPTSDAVVAVVTNGCPTNVIPSAIFVSPGSTCVTFQVQRLLSCNKVYIHARLNVCSVSAQQICSTSPSVRKCPSNQRGRRSIQEEEVFGPLFILPERKIPIEKPAEVENFDAKNLILISLLGVLAVFVLIASVFLLVSLYRSYRKNNYSVSGRSKRVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>F6SZ37</accession>
    <name>F6SZ37_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 4">Vacuolar protein sorting-associated protein 16 homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">wild type</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport and autophagic pathways. Believed to act as a core component of the putative HOPS and CORVET endosomal tethering complexes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Core component of at least two putative endosomal tethering complexes, the homotypic fusion and vacuole protein sorting (HOPS) complex and the class C core vacuole/endosome tethering (CORVET) complex. Their common core is composed of the class C Vps proteins VPS11, VPS16, VPS18 and VPS33A, which in HOPS further associates with VPS39 and VPS41 and in CORVET with VPS8 and TGFBRAP1. Interacts with RAB5C. Interacts with STX17, MON1B. Associates with adapter protein complex 3 (AP-3) and clathrin:AP-3 complexes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Late endosome membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
            <orientation evidence="4">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Lysosome membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
            <orientation evidence="4">Cytoplasmic side</orientation>
        </subcellularLocation>
        <text evidence="4">Cytoplasmic, peripheral membrane protein associated with late endosomes/lysosomes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the VPS16 family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002741">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="EAAA01002742">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6SZ37"/>
    <dbReference type="FunCoup" id="F6SZ37">
        <property type="interactions" value="1149"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000014316"/>
    <dbReference type="Ensembl" id="ENSCINT00000014316.3">
        <property type="protein sequence ID" value="ENSCINP00000014316.3"/>
        <property type="gene ID" value="ENSCING00000006973.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000003896"/>
    <dbReference type="HOGENOM" id="CLU_008909_0_0_1"/>
    <dbReference type="InParanoid" id="F6SZ37"/>
    <dbReference type="OMA" id="WCGDDCL"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033263">
        <property type="term" value="C:CORVET complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030897">
        <property type="term" value="C:HOPS complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042144">
        <property type="term" value="P:vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.780:FF:000001">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 16 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.780">
        <property type="entry name" value="Vps16, C-terminal region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016534">
        <property type="entry name" value="VPS16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006925">
        <property type="entry name" value="Vps16_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038132">
        <property type="entry name" value="Vps16_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006926">
        <property type="entry name" value="Vps16_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12811">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING VPS16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12811:SF0">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 16 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04840">
        <property type="entry name" value="Vps16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04841">
        <property type="entry name" value="Vps16_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF007949">
        <property type="entry name" value="VPS16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0967">Endosome</keyword>
    <keyword evidence="4" id="KW-0458">Lysosome</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Vps16 N-terminal" evidence="6">
        <location>
            <begin position="2"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="domain" description="Vps16 C-terminal" evidence="5">
        <location>
            <begin position="524"/>
            <end position="838"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00061859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR007949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000014316.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="844" mass="95750" checksum="5A0E3D878C322CE2" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>F6T2M3</accession>
    <name>F6T2M3_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Thrombospondin Bii</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">thbsb</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">wild type</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the thrombospondin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002292">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6T2M3"/>
    <dbReference type="STRING" id="7719.ENSCINP00000005599"/>
    <dbReference type="Ensembl" id="ENSCINT00000005599.3">
        <property type="protein sequence ID" value="ENSCINP00000005599.3"/>
        <property type="gene ID" value="ENSCING00000002742.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000168527"/>
    <dbReference type="HOGENOM" id="CLU_009257_1_1_1"/>
    <dbReference type="InParanoid" id="F6T2M3"/>
    <dbReference type="OMA" id="IPIGHEC"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd16076">
        <property type="entry name" value="TSPcc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1080.10:FF:000004">
        <property type="entry name" value="Cartilage oligomeric matrix protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000038">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000025">
        <property type="entry name" value="Thrombospondin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000027">
        <property type="entry name" value="Thrombospondin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000002">
        <property type="entry name" value="Thrombospondin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1080.10:FF:000001">
        <property type="entry name" value="Thrombospondin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000170">
        <property type="entry name" value="thrombospondin-3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1080.10">
        <property type="entry name" value="TSP type-3 repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003367">
        <property type="entry name" value="Thrombospondin_3-like_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017897">
        <property type="entry name" value="Thrombospondin_3_rpt"/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
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                <person name="De Tomaso A."/>
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                <person name="Goodstein D.M."/>
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                <person name="Ho I."/>
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                <person name="Huang W."/>
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                <person name="Zhang C."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
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                <person name="Shoguchi E."/>
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                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
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                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1">wild type</strain>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="interactions" value="72"/>
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    <dbReference type="STRING" id="7719.ENSCINP00000022017"/>
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        <property type="protein sequence ID" value="ENSCINP00000022017.2"/>
        <property type="gene ID" value="ENSCING00000011553.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157018"/>
    <dbReference type="HOGENOM" id="CLU_029499_3_0_1"/>
    <dbReference type="InParanoid" id="F6TC27"/>
    <dbReference type="OMA" id="MPVPNWW"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06428">
        <property type="entry name" value="M1P_guanylylT_A_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GMPPB_C"/>
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        <property type="entry name" value="Mannose-1P_guanyltransferase"/>
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        <property type="entry name" value="NTP_transferase_dom"/>
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    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
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        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
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        <property type="entry name" value="SUGAR-1-PHOSPHATE GUANYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25087">
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        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="Trimeric LpxA-like enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nucleotidyl transferase" evidence="2">
        <location>
            <begin position="3"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="Mannose-1-phosphate guanyltransferase C-terminal" evidence="3">
        <location>
            <begin position="287"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000022017.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="425" mass="46789" checksum="7158ED4C04094E4F" modified="2012-04-18" version="2">MWKAIILIGGPEKGTRFRPLSLDVPKPLFPVAGFPMVQHHIEACCQVPEIKEILLIGFFPLSDELKSFLEYAKKQFTPSIRYLQEYTSLGTAGGLYHFRDQIGAGNPSAFLVFNADVCCNFPLQEILSFHKSKTTDDGITIVATEATQQESMSYGCVVEDAATGEATHYVEKPETFVSSLINCGVYAFSPKVLQYMENVFKEHQSERFDSGDDNADQSFSERISLEKEILNPYAGSGKMFVYKTKEVWGQIKSAASAIHANKLYLSLYHKTNPERLAQNSHKQSPTIIGDVFIHPNAEVHSSAVIGPNVTIDDRVVVGPGVRVSNSILLPGAVLKNHSCVLNAIIGWQSIVGDWSRVEGTAHQPDPNAPFSKIISDGLFNEDGRLCPSITVLGYNVFIAPEIIILNSIVLPNKTIQSSSKNLIIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>F6TF08</accession>
    <name>F6TF08_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="F6TF08"/>
    <dbReference type="STRING" id="7719.ENSCINP00000009293"/>
    <dbReference type="Ensembl" id="ENSCINT00000009293.3">
        <property type="protein sequence ID" value="ENSCINP00000009293.3"/>
        <property type="gene ID" value="ENSCING00000004497.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160689"/>
    <dbReference type="HOGENOM" id="CLU_001570_4_1_1"/>
    <dbReference type="InParanoid" id="F6TF08"/>
    <dbReference type="OMA" id="MFHKATE"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016712">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008395">
        <property type="term" value="F:steroid hydroxylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008202">
        <property type="term" value="P:steroid metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006805">
        <property type="term" value="P:xenobiotic metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000094">
        <property type="entry name" value="cytochrome P450 2J6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050182">
        <property type="entry name" value="Cytochrome_P450_fam2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300:SF397">
        <property type="entry name" value="CYTOCHROME P450 2U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300">
        <property type="entry name" value="CYTOCHROME P450 508A4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0349">Heme</keyword>
    <keyword evidence="4 5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="3 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="5">
        <location>
            <position position="420"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000009293.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="474" mass="53570" checksum="1C6F20F410D5A2FF" modified="2012-04-18" version="2">NNVYRFKEDVPPKSLPGPWGFPFVGSAFHLGLHKDKTLYEWTELYGPVYALNLGTRPAVALHGYSAIHEALVDKASSCSGRICAPIIDAMVNQQGLFFVDHDEHWKKWREIAHSGLSNSWKIVSATQSGILTESERLKNYLSEADGKPINIEETLQITMANAVFQTCLSRRFDLDDTEAANFLVDLYATNRDPKFGKILFLTFLRFLPSYKSYLIEGDTRRKKVRKVLRGFIEERLEDDDQSRDLVSLFFKGKTSGPTPSLENKTLVAGLIQDMLFAGSITTSSQLLWVILFVLKNPACMHKMQNELDAVLGDCENPTKSAISDKYDELNYTRAVVQEAMRLRCIAPTSMFHKATEDTTIAGYFVAKDTVIMPNLYSVHHDPITWAPDPESFRPERHLDSDGSFVNSDHVIPFSVGWRRCVGEKIARTEIFAHLVTIFKHFEVALHPDDVGVDMEGVPGPILRPVSYRTQFTSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>F6TK16</accession>
    <accession>A0A1W2WMZ3</accession>
    <name>F6TK16_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Very-long-chain 3-oxoacyl-CoA reductase-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100176329</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">wild type</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001703">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="RefSeq" id="XP_002130556.1">
        <property type="nucleotide sequence ID" value="XM_002130520.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6TK16"/>
    <dbReference type="FunCoup" id="F6TK16">
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    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000019458"/>
    <dbReference type="Ensembl" id="ENSCINT00000019458.3">
        <property type="protein sequence ID" value="ENSCINP00000019458.3"/>
        <property type="gene ID" value="ENSCING00000009567.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100176329"/>
    <dbReference type="KEGG" id="cin:100176329"/>
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    <dbReference type="HOGENOM" id="CLU_010194_38_0_1"/>
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    <dbReference type="OMA" id="CKASINF"/>
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        <property type="component" value="Chromosome 3"/>
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        <property type="evidence" value="ECO:0000318"/>
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    <dbReference type="GO" id="GO:0006694">
        <property type="term" value="P:steroid biosynthetic process"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="CDD" id="cd05356">
        <property type="entry name" value="17beta-HSD1_like_SDR_c"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Hydroxysteroid (17-beta) dehydrogenase 3"/>
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        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
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        <property type="entry name" value="SDR_fam"/>
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        <property type="entry name" value="VLCFA-Steroid_DH"/>
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        <property type="entry name" value="RH59310P"/>
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        <property type="entry name" value="RH59310P"/>
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        <property type="entry name" value="11-beta-HSD1"/>
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        <property type="entry name" value="GDHRDH"/>
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        <property type="entry name" value="SDRFAMILY"/>
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    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="3" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0752">Steroid biosynthesis</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000019458.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="310" mass="34547" checksum="EF04AEB795583565" modified="2012-04-18" version="2">MECWLFTYIGIATCSIVAFKFVSKIVHALRIYVFPTVPKFSKYGKWTVVTGCTSGIGKSIAKALAARGQNIALISRNPEKLKTVATELETKYNVQTKYLVIDFTQDESIYEKIEEFLQGMDIGTLVNNVGMASPLAFYLDTKNLSQILPAIMKVNVMSVFKMTQIVLPGMMERKRGLILNISSASSLVPVNGFSVYGATKALVNYFSKCISRECEGHGITVQSVKPFFVSTNMVNNVKPNMLVMDADYYVNSLLGTIGKERESDGCWQHGLQGFFVRHMTEGTFNALMKRRMKTMQRNLSLAGKAAKKDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>F6TXT5</accession>
    <name>F6TXT5_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA-(apurinic or apyrimidinic site) lyase</fullName>
            <ecNumber evidence="3">4.2.99.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">40S ribosomal protein S3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100176033</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">wild type</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'-deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3-dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-phospho-2'-deoxyribonucleoside-DNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66592"/>
            <dbReference type="Rhea" id="RHEA-COMP:13180"/>
            <dbReference type="Rhea" id="RHEA-COMP:16897"/>
            <dbReference type="Rhea" id="RHEA-COMP:17067"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:136412"/>
            <dbReference type="ChEBI" id="CHEBI:157695"/>
            <dbReference type="ChEBI" id="CHEBI:167181"/>
            <dbReference type="EC" id="4.2.99.18"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the universal ribosomal protein uS3 family.</text>
    </comment>
    <dbReference type="EC" id="4.2.99.18" evidence="3"/>
    <dbReference type="EMBL" id="EAAA01001422">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6TXT5"/>
    <dbReference type="FunCoup" id="F6TXT5">
        <property type="interactions" value="626"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000010313"/>
    <dbReference type="Ensembl" id="ENSCINT00000010313.3">
        <property type="protein sequence ID" value="ENSCINP00000010313.3"/>
        <property type="gene ID" value="ENSCING00000005003.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000008610"/>
    <dbReference type="HOGENOM" id="CLU_058591_2_1_1"/>
    <dbReference type="InParanoid" id="F6TXT5"/>
    <dbReference type="OMA" id="NKKKWMI"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140078">
        <property type="term" value="F:class I DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001235">
        <property type="term" value="P:positive regulation of apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02413">
        <property type="entry name" value="KH-II_40S_S3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="40S ribosomal protein S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.20:FF:000006">
        <property type="entry name" value="40S ribosomal protein S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1140.32">
        <property type="entry name" value="Ribosomal protein S3, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004044">
        <property type="entry name" value="KH_dom_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036419">
        <property type="entry name" value="Ribosomal_S3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057258">
        <property type="entry name" value="Ribosomal_uS3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001351">
        <property type="entry name" value="Ribosomal_uS3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018280">
        <property type="entry name" value="Ribosomal_uS3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005703">
        <property type="entry name" value="Ribosomal_uS3_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003219">
        <property type="entry name" value="PRK04191.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01008">
        <property type="entry name" value="uS3_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11760">
        <property type="entry name" value="30S/40S RIBOSOMAL PROTEIN S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11760:SF32">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07650">
        <property type="entry name" value="KH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00189">
        <property type="entry name" value="Ribosomal_S3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="Prokaryotic type KH domain (KH-domain type II)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54821">
        <property type="entry name" value="Ribosomal protein S3 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50823">
        <property type="entry name" value="KH_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00548">
        <property type="entry name" value="RIBOSOMAL_S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12" id="KW-0687">Ribonucleoprotein</keyword>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
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                <person name="Matsumoto J."/>
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                <person name="Wiley G.B."/>
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                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
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                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
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                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
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                <person name="Kano S."/>
                <person name="Kobayashi K."/>
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                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
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                <consortium name="Ensembl"/>
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    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000005219.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="211" mass="23913" checksum="6ACD62AD83C2ACDD" modified="2011-07-27" version="1">MSGGLAPSKSTVYVSNLPFSLTNNDLHKIFGKMGKVAKVTVTKNKEDRESTGLAFVLYLKKEDAMKAVHIMDGKQLLGRKLKCSIAKDNGRTKEFIKRKEYKDKSRCYECGDFDHLSYNCPKNKFGDREPPPKKKRSRKPQVTFHNMKRTKQAKPVIEPEDSDGSGEDPSLESLSCVIAEESMKASRSDVVISSRKKYKKDSYFSDEEDLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>F6UMZ6</accession>
    <name>F6UMZ6_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Exocyst complex component Sec3 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01002819">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6UMZ6"/>
    <dbReference type="STRING" id="7719.ENSCINP00000025940"/>
    <dbReference type="Ensembl" id="ENSCINT00000026186.2">
        <property type="protein sequence ID" value="ENSCINP00000025940.2"/>
        <property type="gene ID" value="ENSCING00000014312.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158640"/>
    <dbReference type="HOGENOM" id="CLU_770602_0_0_1"/>
    <dbReference type="InParanoid" id="F6UMZ6"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028258">
        <property type="entry name" value="Sec3-PIP2_bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019160">
        <property type="entry name" value="Sec3_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16092:SF14">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 1 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16092">
        <property type="entry name" value="SEC3/SYNTAXIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15277">
        <property type="entry name" value="Sec3-PIP2_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09763">
        <property type="entry name" value="Sec3_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exocyst complex component Sec3 PIP2-binding N-terminal" evidence="2">
        <location>
            <begin position="2"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="domain" description="Exocyst complex component Sec3 coiled-coil" evidence="1">
        <location>
            <begin position="114"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF15277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000025940.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="360" mass="40510" checksum="201BAF54B12A71AC" modified="2012-04-18" version="2">QESTEFDLQFEKNFKWSATSVGEKHNFLSCLWKLCHRYLPRQQKPAFSNVPQSILEDVLVNLSVMSVAGGDTTRGGSTAGVEDDSLEDYQALTEQEETDLERMITECEFAISDAEAFTNSLSKDLSVLDGQNIHSIMGSEAQVNSLMVLLDNALAEVSQIESRLDEYDHMVGGIATQMGQMKNQESFIQITNKNHTRLLEQLDTLVVQLDLSKGHVKALRESDLRTDSGIEACTSAAEALQEAMEVELPKGLSKLNAVKEQQKLFHDLRQKFAARIFEHLNNVFVRQGSDQPGASLAKQASGLKLPKHFPYHRDLLPYRQLILWLKVADKTKYTQLSTTYTQSLNKLYTQEIKDFMATAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>F6UNZ9</accession>
    <name>F6UNZ9_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidyl-prolyl cis-trans isomerase</fullName>
            <shortName evidence="3">PPIase</shortName>
            <ecNumber evidence="3">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">wild type</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 3">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cyclophilin-type PPIase family.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="3"/>
    <dbReference type="EMBL" id="EAAA01002836">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6UNZ9"/>
    <dbReference type="STRING" id="7719.ENSCINP00000025923"/>
    <dbReference type="Ensembl" id="ENSCINT00000026169.2">
        <property type="protein sequence ID" value="ENSCINP00000025923.2"/>
        <property type="gene ID" value="ENSCING00000014299.2"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012062_38_2_1"/>
    <dbReference type="InParanoid" id="F6UNZ9"/>
    <dbReference type="OMA" id="NCGELLM"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000077">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044666">
        <property type="entry name" value="Cyclophilin_A-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625:SF4">
        <property type="entry name" value="PEPTIDYLPROLYL ISOMERASE DOMAIN AND WD REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="4">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU363019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
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                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
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                <person name="Goodstein D.M."/>
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                <person name="Lemaire P."/>
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                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
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                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
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                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
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                <person name="Richardson P."/>
                <person name="Lucas S."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
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                <person name="Putnam N."/>
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                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
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                <person name="Doggett N."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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            <authorList>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Makabe K.W."/>
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                <person name="Mochizuki Y."/>
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                <person name="Miura S."/>
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                <person name="Nomoto H."/>
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                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
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                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="4" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="4" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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    <feature type="domain" description="BTB" evidence="3">
        <location>
            <begin position="12"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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    </evidence>
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        <source>
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    <accession>A0A1W3JPZ3</accession>
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        <name type="scientific">Ciona intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ciona</taxon>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NPR2 family.</text>
    </comment>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_009861032.3"/>
    </dbReference>
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        <property type="interactions" value="14"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000024345"/>
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        <property type="protein sequence ID" value="ENSCINP00000024345.2"/>
        <property type="gene ID" value="ENSCING00000013218.2"/>
    </dbReference>
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    <dbReference type="KEGG" id="cin:100183442"/>
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    <dbReference type="OMA" id="IVMHKPD"/>
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        <property type="component" value="Chromosome 8"/>
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        <property type="term" value="C:GATOR1 complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="C:vacuolar membrane"/>
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        <property type="term" value="P:cellular response to amino acid starvation"/>
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        <property type="term" value="P:negative regulation of TORC1 signaling"/>
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        <property type="term" value="P:positive regulation of autophagy"/>
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        <property type="entry name" value="NPR2-like"/>
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        <property type="entry name" value="NITROGEN PERMEASE REGULATOR 2/TUMOR SUPPRESSOR CANDIDATE 4"/>
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        </source>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000024345.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="392" mass="45211" checksum="0588088A4A8AECE5" modified="2012-04-18" version="2">MNLAKKYIKEHPIECFFFSEFHHKQGPKITYQYPLGCITKNSSLLEAVINYVIPKPDLQDKLITVNAVGKKVIGCPVCIDDPKYPRNQYMFNFGLVLDNVHKSSPCIPLIKKLSSYMTELELECYFVSNEKTKEEIPGLLKKIFTELNNEGTCSTSVIDTTTLYLKVFPPSTQPKPVHDHDVPILTVPKDYFKLDKWDLTTQQILPFIDGFKHVQKIAVLADVDAELTRICIQNLAFYEHVQLISIYQYSNVYITTAKLNQFRQNMEMQNECLAYVSRSGHQPPTLHDVMTLYCGLGPRVTLRKLCILFHEQITRVNEQKLIQYGLIHNLIRRINMYPVKVKSERSHNTQSDLHQYTDGLHSADEICCKLGCSHSELLAKLEHDKNIVICWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
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    <accession>A0A1W2WCM1</accession>
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            <fullName evidence="2">Isochorismatase domain-containing protein 1</fullName>
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        <name evidence="4" type="primary">LOC100185313</name>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
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            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
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    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the isochorismatase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002128918.1">
        <property type="nucleotide sequence ID" value="XM_002128882.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6VBS7"/>
    <dbReference type="FunCoup" id="F6VBS7">
        <property type="interactions" value="309"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000003182"/>
    <dbReference type="Ensembl" id="ENSCINT00000003182.3">
        <property type="protein sequence ID" value="ENSCINP00000003182.3"/>
        <property type="gene ID" value="ENSCING00000001591.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100185313"/>
    <dbReference type="KEGG" id="cin:100185313"/>
    <dbReference type="GeneTree" id="ENSGT00390000006753"/>
    <dbReference type="HOGENOM" id="CLU_047255_0_0_1"/>
    <dbReference type="InParanoid" id="F6VBS7"/>
    <dbReference type="OMA" id="VFNNEAN"/>
    <dbReference type="OrthoDB" id="269496at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01012">
        <property type="entry name" value="YcaC_related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.850:FF:000001">
        <property type="entry name" value="Isochorismatase domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.850">
        <property type="entry name" value="Isochorismatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000868">
        <property type="entry name" value="Isochorismatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036380">
        <property type="entry name" value="Isochorismatase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050993">
        <property type="entry name" value="Isochorismatase_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14119">
        <property type="entry name" value="HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14119:SF17">
        <property type="entry name" value="ISOCHORISMATASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00857">
        <property type="entry name" value="Isochorismatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52499">
        <property type="entry name" value="Isochorismatase-like hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Isochorismatase-like" evidence="3">
        <location>
            <begin position="87"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00040688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000003182.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="270" mass="30108" checksum="98A424289091BC30" modified="2012-04-18" version="2">MTSVRCLFICGDETKVCELNTGSGKSDICSEFRKSFDLLNFELQTWDKSWNVWVDVGDEFTLEDSTKFRTRQTNLGGLGNLEASSSVVFCCDMQERFRPAIKYFDEIVQVGGRVVKAAQILNIPLVVTEQYPKGLGSTVKELHISGPDVLAAKTKFSMLTTEVESFLRSNQFKSVLLFGVETHVCVQQTAIDLISRGYEVHLVADATSSRSLSDRQLAFQRVRKLGVFVTSAETILLQLVADKNHGKFKEIQNLIKVLPPDTGLTPKSNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>F6VFD2</accession>
    <name>F6VFD2_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein lin-7 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100181021</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Plays a role in establishing and maintaining the asymmetric distribution of channels and receptors at the plasma membrane of polarized cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 10">Cell membrane</location>
            <topology evidence="1 10">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the lin-7 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_026695849.1">
        <property type="nucleotide sequence ID" value="XM_026840048.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6VFD2"/>
    <dbReference type="FunCoup" id="F6VFD2">
        <property type="interactions" value="75"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000016651"/>
    <dbReference type="Ensembl" id="ENSCINT00000016651.3">
        <property type="protein sequence ID" value="ENSCINP00000016651.3"/>
        <property type="gene ID" value="ENSCING00000011254.2"/>
    </dbReference>
    <dbReference type="GeneID" id="100181021"/>
    <dbReference type="GeneTree" id="ENSGT00940000153222"/>
    <dbReference type="HOGENOM" id="CLU_097962_0_0_1"/>
    <dbReference type="InParanoid" id="F6VFD2"/>
    <dbReference type="OMA" id="RFDKQRM"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005911">
        <property type="term" value="C:cell-cell junction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097025">
        <property type="term" value="C:MPP7-DLG1-LIN7 complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007269">
        <property type="term" value="P:neurotransmitter secretion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008582">
        <property type="term" value="P:regulation of synaptic assembly at neuromuscular junction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048489">
        <property type="term" value="P:synaptic vesicle transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06796">
        <property type="entry name" value="PDZ_Lin-7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000076">
        <property type="entry name" value="Protein lin-7 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.650">
        <property type="entry name" value="L27 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014775">
        <property type="entry name" value="L27_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004172">
        <property type="entry name" value="L27_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036892">
        <property type="entry name" value="L27_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017365">
        <property type="entry name" value="LIN7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051109">
        <property type="entry name" value="MAM_complex_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14063">
        <property type="entry name" value="PROTEIN LIN-7 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02828">
        <property type="entry name" value="L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038039">
        <property type="entry name" value="Lin-7_homologue"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00569">
        <property type="entry name" value="L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101288">
        <property type="entry name" value="L27 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51022">
        <property type="entry name" value="L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0965">Cell junction</keyword>
    <keyword evidence="5 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 10" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0813">Transport</keyword>
    <feature type="domain" description="L27" evidence="12">
        <location>
            <begin position="10"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="11">
        <location>
            <begin position="93"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR038039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000016651.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="200" mass="21938" checksum="CAC0540948E39C21" modified="2012-04-18" version="2">MALTAEPLTLERDINRACELIDILKSSGQVPTQKLEQLQAVLRSDFLQAVREVYEHVYETVEISGSPEIAANATAKATVAAFAASEGHSHPRVIEMPKTEEGLGFNVMGGKEQSSPIYISRIIPGGVADRHGALKRGDQLLSVNGVSVEGECHEKAVDLLKAAQGSVKLVVRYTPRVLEEMENRFERMRLSRRQNQNSTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>F6VQ10</accession>
    <name>F6VQ10_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 13">FACT complex subunit SSRP1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">wild type</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Chromosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the SSRP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001759">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6VQ10"/>
    <dbReference type="FunCoup" id="F6VQ10">
        <property type="interactions" value="616"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSCINT00000000932.4">
        <property type="protein sequence ID" value="ENSCINP00000000932.4"/>
        <property type="gene ID" value="ENSCING00000000505.4"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157117"/>
    <dbReference type="HOGENOM" id="CLU_017374_2_1_1"/>
    <dbReference type="InParanoid" id="F6VQ10"/>
    <dbReference type="OMA" id="QVVTKIF"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035101">
        <property type="term" value="C:FACT complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045787">
        <property type="term" value="P:positive regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902275">
        <property type="term" value="P:regulation of chromatin organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045995">
        <property type="term" value="P:regulation of embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd21994">
        <property type="entry name" value="HMG-box_SSRP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13230">
        <property type="entry name" value="PH1_SSRP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13231">
        <property type="entry name" value="PH2_SSRP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.150:FF:000001">
        <property type="entry name" value="Fact complex subunit ssrp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.220:FF:000001">
        <property type="entry name" value="FACT complex subunit SSRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000119">
        <property type="entry name" value="FACT complex subunit SSRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000214">
        <property type="entry name" value="FACT complex subunit SSRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.220">
        <property type="entry name" value="Structure-specific recognition protein (SSRP1)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013719">
        <property type="entry name" value="RTT106/SPT16-like_middle_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050454">
        <property type="entry name" value="RTT106/SSRP1_HistChap/FACT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048993">
        <property type="entry name" value="SSRP1-like_PH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000969">
        <property type="entry name" value="SSRP1/POB3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035417">
        <property type="entry name" value="SSRP1/POB3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024954">
        <property type="entry name" value="SSRP1_DD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038167">
        <property type="entry name" value="SSRP1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45849">
        <property type="entry name" value="FACT COMPLEX SUBUNIT SSRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45849:SF1">
        <property type="entry name" value="FACT COMPLEX SUBUNIT SSRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21103">
        <property type="entry name" value="PH1_SSRP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17292">
        <property type="entry name" value="POB3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08512">
        <property type="entry name" value="Rttp106-like_middle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03531">
        <property type="entry name" value="SSrecog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00887">
        <property type="entry name" value="SSRCOGNITION"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01287">
        <property type="entry name" value="Rtt106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 13" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6 13" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10 13" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5 13" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="11 12 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 13" id="KW-0804">Transcription</keyword>
    <keyword evidence="7 13" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HMG box" evidence="15">
        <location>
            <begin position="554"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="12">
        <location>
            <begin position="554"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="460"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="460"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="470"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="508"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="531"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU364013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000000932.4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="603" mass="70645" checksum="804C33A362F674D6" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>F6W2W3</accession>
    <name>F6W2W3_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein 110</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
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    <dbReference type="AlphaFoldDB" id="F6XKI3"/>
    <dbReference type="STRING" id="7719.ENSCINP00000021332"/>
    <dbReference type="Ensembl" id="ENSCINT00000021332.3">
        <property type="protein sequence ID" value="ENSCINP00000021332.3"/>
        <property type="gene ID" value="ENSCING00000010797.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165975"/>
    <dbReference type="HOGENOM" id="CLU_099886_0_0_1"/>
    <dbReference type="InParanoid" id="F6XKI3"/>
    <dbReference type="OMA" id="VDDIMIC"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000417">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050127">
        <property type="entry name" value="Serine_Proteases_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24264:SF54">
        <property type="entry name" value="PEPTIDASE S1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24264">
        <property type="entry name" value="TRYPSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="Peptidase S1" evidence="4">
        <location>
            <begin position="1"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000021332.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="243" mass="27169" checksum="D91A94160F8C8638" modified="2011-07-27" version="1">MVVYNTEKIIYHPDFDDISLDNDIALVKLGQQVKLDPTKTSWINVPNTFGWVNFTDYIRPVCLPCMPNNCLNSYLRTKGRIPANSNQKQICDIETAAVLDVSNNQNIAVVTGFGHENERSHNDLKLNASATLKQGVLKLMPHATCRDFTNQWGTDLTQRMVCAPSANDTVGTDACKGDSGGPLIRELYDENTRKSCWIQMGIVSWGYGCGKKTMVNGVNRFRPGIFTKLPLFMAWVNQTMEAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>F6XQ16</accession>
    <name>F6XQ16_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 16">Protein kinase C</fullName>
            <ecNumber evidence="2 16">2.7.11.13</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="27 28">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="27">wild type</strain>
        </source>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.13"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="20">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="20">Binds 3 Ca(2+) ions per subunit. The ions are bound to the C2 domain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 16">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.13" evidence="2 16"/>
    <dbReference type="EMBL" id="EAAA01001825">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6XQ16"/>
    <dbReference type="FunCoup" id="F6XQ16">
        <property type="interactions" value="9"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000017438"/>
    <dbReference type="Ensembl" id="ENSCINT00000017438.3">
        <property type="protein sequence ID" value="ENSCINP00000017438.3"/>
        <property type="gene ID" value="ENSCING00000008551.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000167637"/>
    <dbReference type="HOGENOM" id="CLU_000288_54_2_1"/>
    <dbReference type="InParanoid" id="F6XQ16"/>
    <dbReference type="OMA" id="CKESMSD"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004697">
        <property type="term" value="F:diacylglycerol-dependent serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd20833">
        <property type="entry name" value="C1_cPKC_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20836">
        <property type="entry name" value="C1_cPKC_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04026">
        <property type="entry name" value="C2_PKC_alpha_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05587">
        <property type="entry name" value="STKc_cPKC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000023">
        <property type="entry name" value="Protein kinase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000218">
        <property type="entry name" value="Protein kinase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000080">
        <property type="entry name" value="Protein kinase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000006">
        <property type="entry name" value="Protein kinase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000052">
        <property type="entry name" value="Protein kinase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020454">
        <property type="entry name" value="DAG/PE-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014375">
        <property type="entry name" value="Protein_kinase_C_a/b/g"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000550">
        <property type="entry name" value="PKC_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00360">
        <property type="entry name" value="C2DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00008">
        <property type="entry name" value="DAGPEDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
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                <person name="Doyle S."/>
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                <person name="Doggett N."/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
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                <person name="Hastings K.E."/>
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                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
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                <person name="Meinertzhagen I.A."/>
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                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">wild type</strain>
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    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
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        <subcellularLocation>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="157" mass="18195" checksum="37DA5B7CED0E81D5" modified="2012-04-18" version="2">MIFTMLLGDTILIDDLDSANQYRNMVVKHTHCPTILTRNGHRIRSNGKFGGNQNRAPAVEKLRGMVFGAPMSEEYATCVKQIEILENIKSVIEEIHSSQEELESLQLETDEMKFKEQEHKEAQERLNAIEKKIGFHNPQRRSLPESTRQTRKRFKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>F6Z1Q3</accession>
    <name>F6Z1Q3_CIOIN</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Saiga H."/>
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                <person name="Terry A."/>
                <person name="Yamada L."/>
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    <accession>F6ZEC7</accession>
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            <fullName evidence="4">Uncharacterized protein</fullName>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
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                <person name="Hino K."/>
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                <person name="Kobayashi K."/>
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                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
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                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
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                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
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                <person name="Zhang C."/>
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                <person name="Richardson P."/>
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                <person name="Kohara Y."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4">wild type</strain>
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        <scope>IDENTIFICATION</scope>
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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    <sequence length="299" mass="32876" checksum="E555C3986A1D3762" modified="2012-04-18" version="2">MSKVRVIMVTWTCDDQHIMTSHSDFSIKVWKVDTASLLHVLKGHEDEAYCLEAHPNDGRILLSAGHDGKIFLWDINDGQQVINFHNLLVGQGHGAVFDAKFSPTGNHIACTDSHGHLILFGFGDGKVYKKVPDEQFFHTDYRPLLRDMYGAVVDEQTHQAPNRMPPPFLVDVDGNPHPAPLQRLVPGRQNITTDALVPVVVAADNGVPLIVNEQPDIVADLSPPLNEVRAPGVQSPTVSPSRNTPLGLRREGEVVGVRNSSSPRSEIASAADLLVVQRRELVPLLKTAVRIFVSLTRTK</sequence>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
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                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
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                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
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                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
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                <person name="Doyle S."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
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                <person name="Wiley G.B."/>
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            <strain evidence="11">wild type</strain>
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        <text evidence="9 10">Subunit b, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. ATP synthase complex consist of a soluble F(1) head domain - the catalytic core - and a membrane F(1) domain - the membrane proton channel. These two domains are linked by a central stalk rotating inside the F(1) region and a stationary peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. In vivo, can only synthesize ATP although its ATP hydrolase activity can be activated artificially in vitro. Part of the complex F(0) domain. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.</text>
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        <text evidence="10">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) and CF(0) have multiple subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Mitochondrion</location>
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        <subcellularLocation>
            <location evidence="10">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the eukaryotic ATPase B chain family.</text>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015986">
        <property type="term" value="P:proton motive force-driven ATP synthesis"/>
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        <property type="project" value="GO_Central"/>
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    <dbReference type="InterPro" id="IPR013837">
        <property type="entry name" value="ATP_synth_F0_suB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12733:SF3">
        <property type="entry name" value="ATP SYNTHASE F(0) COMPLEX SUBUNIT B1, MITOCHONDRIAL"/>
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    </dbReference>
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        <property type="entry name" value="MITOCHONDRIAL ATP SYNTHASE B CHAIN"/>
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    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
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    <keyword evidence="2 10" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU368017"/>
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    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000027548.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="135" mass="15307" checksum="BA8EFC7F47E9B917" modified="2012-04-18" version="2">MAGKDIRNLFDNMRTDELKALDQVKSDQMEDLKQTVDEIKLEQWRSGSAEMINDALKNNLAMMLETEYLNRQAAVVEAVKRKLDYQVEVQKVDSQLAHGHMVKWIEEKVMNTITPASQKATLAACIAQLNAMPGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>F6ZN43</accession>
    <name>F6ZN43_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">BING4 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">wild type</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="EAAA01002897">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6ZN43"/>
    <dbReference type="FunCoup" id="F6ZN43">
        <property type="interactions" value="824"/>
    </dbReference>
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    <dbReference type="Ensembl" id="ENSCINT00000006950.3">
        <property type="protein sequence ID" value="ENSCINP00000006950.3"/>
        <property type="gene ID" value="ENSCING00000003393.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000007075"/>
    <dbReference type="HOGENOM" id="CLU_022996_0_0_1"/>
    <dbReference type="InParanoid" id="F6ZN43"/>
    <dbReference type="OMA" id="QEANMQV"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012952">
        <property type="entry name" value="BING4_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040315">
        <property type="entry name" value="WDR46/Utp7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14085:SF3">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14085">
        <property type="entry name" value="WD-REPEAT PROTEIN BING4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08149">
        <property type="entry name" value="BING4CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BING4CT"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="WD40 repeat-like"/>
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    </dbReference>
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        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="2 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="84"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="BING4 C-terminal" evidence="7">
        <location>
            <begin position="165"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="272"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="276"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="298"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM01033"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000006950.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="351" mass="40139" checksum="CE61D2A5E3C7852C" modified="2012-04-18" version="2">MKRLNDTLRLDFLPYHFLLTTTNRHGYLQYTDISTGNIVSTINTKGGRLNVMCHNPHNAVVLLGHHNGSISMWSPNQKEPLVRMLCHKTAIRSMAVEKRGNYLATAGQDRKMKIFDLRMYKPLHSYQLSTGPSNLCFSQRNLLAATSNNVVEIYNDPCIQVQERPYLVHKMKLPAEDIAFCPYEDVLGVGAADGFVSLLVPGAGEPNYDAFEANPNRSKQQRREWEVKALLEKIQPEMISLDPFMLSKVDHVTAERLRKDREEILGYVPDKPAFAPRHKKKGRSKSGHVEKRKKKVKGEKLRNHIRDEMKTKREMLKKDERKAGRLPHTVTTDGSQVEPAIMKSALDRFKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>F6ZSI5</accession>
    <name>F6ZSI5_CIOIN</name>
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        <recommendedName>
            <fullName evidence="4">Thrombospondin-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
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            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">wild type</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001000">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_026692964.1">
        <property type="nucleotide sequence ID" value="XM_026837163.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_026692965.1">
        <property type="nucleotide sequence ID" value="XM_026837164.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F7A7A0"/>
    <dbReference type="FunCoup" id="F7A7A0">
        <property type="interactions" value="131"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000025772"/>
    <dbReference type="Ensembl" id="ENSCINT00000026018.2">
        <property type="protein sequence ID" value="ENSCINP00000025772.2"/>
        <property type="gene ID" value="ENSCING00000014190.2"/>
    </dbReference>
    <dbReference type="GeneID" id="100178763"/>
    <dbReference type="KEGG" id="cin:100178763"/>
    <dbReference type="GeneTree" id="ENSGT00940000170174"/>
    <dbReference type="HOGENOM" id="CLU_019796_1_2_1"/>
    <dbReference type="InParanoid" id="F7A7A0"/>
    <dbReference type="OMA" id="QITPHNA"/>
    <dbReference type="OrthoDB" id="298012at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030267">
        <property type="term" value="F:glyoxylate reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008465">
        <property type="term" value="F:hydroxypyruvate reductase (NADH) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05301">
        <property type="entry name" value="GDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000026">
        <property type="entry name" value="Glyoxylate/hydroxypyruvate reductase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050223">
        <property type="entry name" value="D-isomer_2-hydroxyacid_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029753">
        <property type="entry name" value="D-isomer_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10996">
        <property type="entry name" value="2-HYDROXYACID DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10996:SF277">
        <property type="entry name" value="GLYOXYLATE REDUCTASE_HYDROXYPYRUVATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00671">
        <property type="entry name" value="D_2_HYDROXYACID_DH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase catalytic" evidence="4">
        <location>
            <begin position="6"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding" evidence="5">
        <location>
            <begin position="111"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00073306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000025772.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="324" mass="35553" checksum="AFDC5DF38CD13159" modified="2012-04-18" version="2">MPKLDILITRNIPGAALKLLRERFKVDIWPSDEVIPRPTLLSKIKDKDGVLCLLTDQINKEVLDKAGERLRVVSTISVGVDHVDTVECTKRGIKVGNTPDILTAATAELTVGLLISTSRRIVEGTHAARNGDWGAWKLMWMCGPTLDGATVGLFGLGRIGTAVVQRLKAFGVKKFLYNTANKKNNNFEKNLGVQFASFHDLLHESDFIISCCALNKSTAGIFNKNAFEKMKNSSIFINISRGGVVNQEDLYHALSHNIIRGAGIDVTTPEPLPTDHPLFSLKNCVVTPHIGSATEKTRMEMTMLAVQNLICGLDGTEMPAEYKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>F7ADM8</accession>
    <name>F7ADM8_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Hexosyltransferase</fullName>
            <ecNumber evidence="9">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Golgi apparatus</location>
            <location evidence="1 9">Golgi stack membrane</location>
            <topology evidence="1 9">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the chondroitin N-acetylgalactosaminyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="9"/>
    <dbReference type="AlphaFoldDB" id="F7ADM8"/>
    <dbReference type="STRING" id="7719.ENSCINP00000007559"/>
    <dbReference type="Ensembl" id="ENSCINT00000007559.3">
        <property type="protein sequence ID" value="ENSCINP00000007559.3"/>
        <property type="gene ID" value="ENSCING00000003670.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244857"/>
    <dbReference type="HOGENOM" id="CLU_025958_1_1_1"/>
    <dbReference type="InParanoid" id="F7ADM8"/>
    <dbReference type="OMA" id="HINVMSI"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008376">
        <property type="term" value="F:acetylgalactosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008428">
        <property type="entry name" value="Chond_GalNAc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051227">
        <property type="entry name" value="CS_glycosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12369">
        <property type="entry name" value="CHONDROITIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12369:SF11">
        <property type="entry name" value="HEXOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05679">
        <property type="entry name" value="CHGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000007559.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="199" mass="22718" checksum="0123C7C9275B0684" modified="2012-04-18" version="2">MIEYIFTKGEFNRAVGLHAGVMQCNDDEIVLFLDVDLKIGGDFLGRVRRNVVQHERAIFPIMFSQYDSDVMKHASTVDMELVQDVEDPMQINKQTGFWIYYAFGVAAMYKSDYLKVGGFRLDIKGWGGEDVDLFKKMISDPHINVMSIVDADLMHIYHKRGCDVTLAPDQYAMCVGAWAETLGSQRQVGTLYLNKHYEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>F7AR48</accession>
    <name>F7AR48_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">UBX domain-containing protein 11</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Socius</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">UBX domain-containing protein 5</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">wild type</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">May be involved in the reorganization of actin cytoskeleton mediated by RND1, RND2 and RND3. Promotes RHOA activation mediated by GNA12 and GNA13.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Interacts with GNA12, GNA13, RND1, RND2 and RND3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="EAAA01001581">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F7AR48"/>
    <dbReference type="STRING" id="7719.ENSCINP00000013759"/>
    <dbReference type="Ensembl" id="ENSCINT00000013759.3">
        <property type="protein sequence ID" value="ENSCINP00000013759.3"/>
        <property type="gene ID" value="ENSCING00000006709.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00520000055567"/>
    <dbReference type="HOGENOM" id="CLU_044433_0_1_1"/>
    <dbReference type="InParanoid" id="F7AR48"/>
    <dbReference type="OMA" id="DFELMSA"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd17077">
        <property type="entry name" value="UBX_UBXN11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.210:FF:000003">
        <property type="entry name" value="UBX domain protein 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000677">
        <property type="entry name" value="UBX domain-containing protein 11-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.210">
        <property type="entry name" value="SEP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036241">
        <property type="entry name" value="NSFL1C_SEP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012989">
        <property type="entry name" value="SEP_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001012">
        <property type="entry name" value="UBX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23333">
        <property type="entry name" value="UBX DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23333:SF4">
        <property type="entry name" value="UBX DOMAIN-CONTAINING PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08059">
        <property type="entry name" value="SEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00789">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00166">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102848">
        <property type="entry name" value="NSFL1 (p97 ATPase) cofactor p47, SEP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51399">
        <property type="entry name" value="SEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50033">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SEP" evidence="11">
        <location>
            <begin position="171"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="UBX" evidence="10">
        <location>
            <begin position="336"/>
            <end position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00059434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00062345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00073759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00075811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00081109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000013759.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="416" mass="47359" checksum="071EE1505BE1D357" modified="2012-04-18" version="2">MPQAPNDMELLSTMMKRITQLEKQVAFYSKEVVEKDTRIKILEDKLALFKNKESKESDEPGKVAQLEMKCLQLQQEIYEMEAFLADYGMIWVGEKSNPNSKEYLENDDDSVPVATVTDGSWAQGKTIYPPPENFKVDYDLVLRNIQELNIIAGDGQADIVKTKGGAKLQAQKTVPLILYANGIMLFNGPFRPLSDVTTQRCLCDITDGYFPSELQERYPDGVPIQVTDKRHIKFKDARKAEIFPGVGRSLLDKDNIAKNLEESSELPGLRMSVEQFLDKVPKSVIRGGKVVDIRSSLKESLTDSTRGKVTTEIVETEAVKNMQHRMEIPQQERPVSARDVTTIRVKSEDGSKIFILKLKFSDTIQDLRNYIKKQRPRHREPYDILSTFPNQKHSDLKKTMQDSGLVPNATLFLKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>F7AU99</accession>
    <name>F7AU99_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Fibronectin type-II domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="16">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="16">Can bind about 5 Ca(2+) ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="16">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M10A family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="F7AU99"/>
    <dbReference type="SMR" id="F7AU99"/>
    <dbReference type="FunCoup" id="F7AU99">
        <property type="interactions" value="11"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000011394"/>
    <dbReference type="Ensembl" id="ENSCINT00000011394.3">
        <property type="protein sequence ID" value="ENSCINP00000011394.3"/>
        <property type="gene ID" value="ENSCING00000005511.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158511"/>
    <dbReference type="HOGENOM" id="CLU_015489_6_2_1"/>
    <dbReference type="InParanoid" id="F7AU99"/>
    <dbReference type="OMA" id="LDEMTMM"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030574">
        <property type="term" value="P:collagen catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00062">
        <property type="entry name" value="FN2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd00094">
        <property type="entry name" value="HX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04278">
        <property type="entry name" value="ZnMc_MMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.110.10.10:FF:000052">
        <property type="entry name" value="72 kDa type IV collagenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.10.10:FF:000001">
        <property type="entry name" value="Fibronectin 1a isoform 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.10.10">
        <property type="entry name" value="Fibronectin, type II, collagen-binding"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.110.10.10">
        <property type="entry name" value="Hemopexin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000562">
        <property type="entry name" value="FN_type2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036943">
        <property type="entry name" value="FN_type2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000585">
        <property type="entry name" value="Hemopexin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036375">
        <property type="entry name" value="Hemopexin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018487">
        <property type="entry name" value="Hemopexin-like_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018486">
        <property type="entry name" value="Hemopexin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013806">
        <property type="entry name" value="Kringle-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033739">
        <property type="entry name" value="M10A_MMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001818">
        <property type="entry name" value="Pept_M10_metallopeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021190">
        <property type="entry name" value="Pept_M10A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021158">
        <property type="entry name" value="Pept_M10A_Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006026">
        <property type="entry name" value="Peptidase_Metallo"/>
    </dbReference>
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        <property type="entry name" value="MATRIX METALLOPROTEINASE-14-LIKE ISOFORM X1"/>
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        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
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        <property type="entry name" value="HEMOPEXIN_2"/>
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    <keyword evidence="4" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 21" id="KW-0732">Signal</keyword>
    <keyword evidence="10 16" id="KW-0862">Zinc</keyword>
    <keyword evidence="14" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014174262" description="Fibronectin type-II domain-containing protein" evidence="21">
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            <begin position="20"/>
            <end position="758"/>
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            <begin position="330"/>
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            <begin position="388"/>
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    <feature type="domain" description="Fibronectin type-II" evidence="22">
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            <begin position="447"/>
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            <begin position="561"/>
            <end position="611"/>
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    <feature type="repeat" description="Hemopexin" evidence="20">
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            <begin position="612"/>
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            <begin position="661"/>
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            <position position="202"/>
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        <ligand>
            <name>Zn(2+)</name>
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            <label>2</label>
            <note>catalytic</note>
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        <ligand>
            <name>Ca(2+)</name>
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        <ligand>
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        <ligand>
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        <location>
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        <ligand>
            <name>Ca(2+)</name>
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        <ligand>
            <name>Ca(2+)</name>
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        </ligand>
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            <begin position="349"/>
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    <feature type="disulfide bond" evidence="19">
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            <begin position="393"/>
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    </feature>
    <feature type="disulfide bond" evidence="19">
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    </feature>
    <feature type="disulfide bond" evidence="19">
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            <end position="478"/>
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    </feature>
    <feature type="disulfide bond" evidence="19">
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            <begin position="466"/>
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    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="564"/>
            <end position="758"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00010370"/>
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            <dbReference type="ARBA" id="ARBA00022525"/>
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            <dbReference type="ARBA" id="ARBA00022530"/>
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            <dbReference type="ARBA" id="ARBA00022670"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023105"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR621190-2"/>
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        <source>
            <dbReference type="PIRSR" id="PIRSR621190-3"/>
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        <source>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01011"/>
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        <source>
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        <source>
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    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    </evidence>
    <sequence length="758" mass="85541" checksum="3C55D976138676ED" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>F7AUM2</accession>
    <accession>A0A1W2WEX6</accession>
    <name>F7AUM2_CIOIN</name>
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        <recommendedName>
            <fullName evidence="4">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="4">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100178049</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">wild type</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the PP2C family.</text>
    </comment>
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        <name type="scientific">Ciona intestinalis</name>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
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                <person name="Haga S."/>
                <person name="Hayashi H."/>
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                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
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            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">wild type</strain>
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    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Di Gregorio A."/>
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                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
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                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
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                <person name="Richardson P."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="7" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">wild type</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the TGF-beta family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01000233">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F7BBL9"/>
    <dbReference type="Ensembl" id="ENSCINT00000018569.3">
        <property type="protein sequence ID" value="ENSCINP00000018569.3"/>
        <property type="gene ID" value="ENSCING00000005309.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00660000096247"/>
    <dbReference type="HOGENOM" id="CLU_619557_0_0_1"/>
    <dbReference type="InParanoid" id="F7BBL9"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030509">
        <property type="term" value="P:BMP signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd13756">
        <property type="entry name" value="TGF_beta_BMPs_GDFs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001839">
        <property type="entry name" value="TGF-b_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015615">
        <property type="entry name" value="TGF-beta-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848">
        <property type="entry name" value="TGF-BETA FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848:SF119">
        <property type="entry name" value="TGF-BETA FAMILY PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00019">
        <property type="entry name" value="TGF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00204">
        <property type="entry name" value="TGFB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51362">
        <property type="entry name" value="TGF_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0339">Growth factor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003348304" description="TGF-beta family profile domain-containing protein" evidence="5">
        <location>
            <begin position="18"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="domain" description="TGF-beta family profile" evidence="6">
        <location>
            <begin position="322"/>
            <end position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000018569.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="438" mass="49507" checksum="0E4355118116BBEF" modified="2012-04-18" version="2">MYCAVIFASYFIAAVAGIHTRHGRTWYTPNHHEESAIERCLRGANVVGSFAARCEVLRVVARDMGNIDTLNRDRRVCRYFPSKGDIGFLELEYVKKLSDIKNRVQPSEVTMENMLDAIAPCSVFSPSLKRKWENFFGPRGGDQPNELITRFPVQHRSEAEHVTVSFTHPPRHNFDLHELIGVDLVLLRTPPCQLNEGFGLYDVTVRKLPNDGSETEILVHHSTQASCASGGGYMMVASLDTGYLMEWLLHEENDLQLSVQVTQRRQVTSGNSSEPKVSNILRRVLSISFHLRAGPGASQQVIVRIPQGRRTHHRQNEGWITRMRRSPSRGIRGCERKSFPINFQNDLFRHHILYPSSVYDIGECVGRCDFPIQMPTNASNHALALTVEREFGLRSENAELTICCVPTDYYSMYATISSELGVESVFLPEIEVKHCGCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>H2XJR2</accession>
    <name>H2XJR2_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Short-chain dehydrogenase/reductase 3</fullName>
            <ecNumber evidence="3">1.1.1.300</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Retinal short-chain dehydrogenase/reductase 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">wild type</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the reduction of all-trans-retinal to all-trans-retinol in the presence of NADPH.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>all-trans-retinol + NADP(+) = all-trans-retinal + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25033"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17336"/>
            <dbReference type="ChEBI" id="CHEBI:17898"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.300"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.300" evidence="3"/>
    <dbReference type="EMBL" id="EAAA01002798">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XJR2"/>
    <dbReference type="FunCoup" id="H2XJR2">
        <property type="interactions" value="11"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000029894"/>
    <dbReference type="Ensembl" id="ENSCINT00000035457.1">
        <property type="protein sequence ID" value="ENSCINP00000029894.1"/>
        <property type="gene ID" value="ENSCING00000024165.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157063"/>
    <dbReference type="HOGENOM" id="CLU_010194_2_5_1"/>
    <dbReference type="InParanoid" id="H2XJR2"/>
    <dbReference type="OMA" id="SVEGQVC"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052650">
        <property type="term" value="F:all-trans-retinol dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd05339">
        <property type="entry name" value="17beta-HSDXI-like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000131">
        <property type="entry name" value="Short-chain dehydrogenase/reductase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24322">
        <property type="entry name" value="PKSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24322:SF746">
        <property type="entry name" value="SHORT CHAIN DEHYDROGENASE_REDUCTASE FAMILY 16C MEMBER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00050568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00059620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00068717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00082544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000029894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="314" mass="35014" checksum="AB6F7211B5470382" modified="2012-04-18" version="1">MSFFMDLIILMYGLTASFFTSFVRWLIPVDHKSVSGEVCLITGAGNGIGRLMAIEFAKRRAKVVLWDFDKEGLKETSAMIRELGMDVYTEVCDVSKKDVIKAAAAKVKQEFGEVNILVNNAGIYTSKMLLDLTEHEIENTYKVNVLAHIWIIREFLPSMMERNHGHIVNVASTVGLFASPGMPDYCSSKHAAVGLHNSLFLDLQNAEKYGVKTTLVCPYLIHTGMFNGVHVKYDWILPPIKPQDCADIIINAVLTEQNMVAIPGTMYIAHILSTILPTRALIAGYKFVGADKAISYFKPNRPYQYSNMDPSSGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>H2XJX9</accession>
    <name>H2XJX9_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tetraspanin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
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                <person name="Satou Y."/>
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                <person name="Chapman J."/>
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                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
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                <person name="Goodstein D.M."/>
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                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
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                <person name="Matsumoto J."/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="De Tomaso A."/>
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                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
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                <person name="Hastings K.E."/>
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                <person name="Kawashima T."/>
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                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
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                <person name="Branno M."/>
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                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Ueno K."/>
                <person name="Yamada L."/>
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                <person name="Roe B.A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01000737">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XMJ4"/>
    <dbReference type="Ensembl" id="ENSCINT00000032456.1">
        <property type="protein sequence ID" value="ENSCINP00000030877.1"/>
        <property type="gene ID" value="ENSCING00000023343.1"/>
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    <dbReference type="HOGENOM" id="CLU_3353319_0_0_1"/>
    <dbReference type="InParanoid" id="H2XMJ4"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000030877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="37" mass="4427" checksum="36385DC3FBE45B24" modified="2012-04-18" version="1">MICKVRNKNDLFYICLENAILTYAMMFSHFLASIFHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>H2XP28</accession>
    <name>H2XP28_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
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            <taxon>Ciona</taxon>
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    <reference evidence="12" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="H2XP28"/>
    <dbReference type="STRING" id="7719.ENSCINP00000031411"/>
    <dbReference type="Ensembl" id="ENSCINT00000032723.1">
        <property type="protein sequence ID" value="ENSCINP00000031411.1"/>
        <property type="gene ID" value="ENSCING00000023238.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154248"/>
    <dbReference type="HOGENOM" id="CLU_565694_0_0_1"/>
    <dbReference type="InParanoid" id="H2XP28"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="F:G protein-coupled peptide receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="P:hormone-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="match status" value="2"/>
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        <property type="entry name" value="AGAP003656-PC"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Low-density lipoprotein receptor-related protein"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
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        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24372:SF77">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 1 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24372">
        <property type="entry name" value="GLYCOPROTEIN HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00261">
        <property type="entry name" value="LDLRECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="4"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="7 8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>H2XQJ1</accession>
    <name>H2XQJ1_CIOIN</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
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    </protein>
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        <name type="common">Transparent sea squirt</name>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    <dbReference type="Ensembl" id="ENSCINT00000031817.1">
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    <dbReference type="InParanoid" id="H2XQJ1"/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
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                <person name="De Tomaso A."/>
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                <person name="Di Gregorio A."/>
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                <person name="Goodstein D.M."/>
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                <person name="Kawashima T."/>
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                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Terry A."/>
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                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
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                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
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                <person name="Inaba K."/>
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            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Degnan B."/>
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                <person name="Gelpke M."/>
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                <person name="Hastings K.E."/>
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                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
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                <person name="Hayashi H."/>
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                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
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                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
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                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
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                <person name="Doggett N."/>
                <person name="Glavina T."/>
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                <person name="Richardson P."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01001562">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <proteinExistence type="predicted"/>
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        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
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        <location>
            <begin position="1"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
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        <source>
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        <source>
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    </evidence>
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    <sequence length="56" mass="6133" checksum="B56365E8F72587E8" modified="2012-04-18" version="1">MSESTNENTSKLNEISDNIPSPVIGQNKPISRSYSGDSFSSYSDEFDSSRSSDPKL</sequence>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
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                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
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                <person name="Tassy O."/>
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                <person name="Yagi K."/>
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                <person name="Zhang C."/>
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                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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            <begin position="1"/>
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    <evidence type="ECO:0000259" key="1">
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    </evidence>
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    </evidence>
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        <source>
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    <sequence length="142" mass="16073" checksum="564D3D1F1B809690" modified="2012-04-18" version="1">MRQEVTRAHTAKGWALDSVVLYNEVTKSMKEDITSPPPAEIGGVYVYGLFLDGAGWDRRNVKLVEPQPKVLFTSMPVVHVYAINTASPNKDDKKGSMNVYHCPVYKKPRRTDLTYVFPLLLRTVQVPEHWTLRGVAILCDTK</sequence>
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            <fullName evidence="7">Guanine nucleotide exchange factor DBS-like</fullName>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
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                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
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                <person name="Doyle S."/>
                <person name="Francino P."/>
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                <person name="Hayashi H."/>
                <person name="Hino K."/>
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                <person name="Inaba K."/>
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                <person name="Kobayashi M."/>
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                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
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                <person name="Suzuki M.M."/>
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                <person name="Zhang C."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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            <authorList>
                <consortium name="Ensembl"/>
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                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="4" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="Ensembl" id="ENSCINT00000031365.1">
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        <property type="gene ID" value="ENSCING00000021726.1"/>
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        <property type="component" value="Chromosome 8"/>
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    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="SRI"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Set2 Rpb1 interacting" evidence="3">
        <location>
            <begin position="2"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000032569.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
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            <fullName evidence="11">5'-3' exoribonuclease</fullName>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
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            <taxon>Ciona</taxon>
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    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">wild type</strain>
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    <reference evidence="15" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <comment type="function">
        <text evidence="11">Possesses 5'-&gt;3' exoribonuclease activity. May promote termination of transcription by RNA polymerase II.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the 5'-3' exonuclease family. XRN2/RAT1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.13.-" evidence="11"/>
    <dbReference type="EMBL" id="EAAA01000791">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="EMBL" id="EAAA01000792">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="STRING" id="7719.ENSCINP00000032654"/>
    <dbReference type="Ensembl" id="ENSCINT00000033909.1">
        <property type="protein sequence ID" value="ENSCINP00000032654.1"/>
        <property type="gene ID" value="ENSCING00000022336.1"/>
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    <dbReference type="GeneTree" id="ENSGT00670000098098"/>
    <dbReference type="HOGENOM" id="CLU_006038_1_2_1"/>
    <dbReference type="InParanoid" id="H2XSM0"/>
    <dbReference type="OMA" id="ITHDMVV"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0004534">
        <property type="term" value="F:5'-3' RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0000956">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18673">
        <property type="entry name" value="PIN_XRN1-2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="3.40.50.12390:FF:000001">
        <property type="entry name" value="5'-3' exoribonuclease"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="3.40.50.12390:FF:000003">
        <property type="entry name" value="5'-3' exoribonuclease"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="1.25.40.1050">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12390">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027073">
        <property type="entry name" value="5_3_exoribonuclease"/>
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        <property type="entry name" value="Xrn1_helical"/>
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    <dbReference type="PANTHER" id="PTHR12341:SF41">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="XRN_N"/>
        <property type="match status" value="1"/>
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    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="1"/>
            <end position="255"/>
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    <feature type="region of interest" description="Disordered" evidence="12">
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    <feature type="region of interest" description="Disordered" evidence="12">
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            <end position="507"/>
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    <feature type="region of interest" description="Disordered" evidence="12">
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            <begin position="781"/>
            <end position="832"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="479"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="798"/>
            <end position="807"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR037239"/>
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    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <topology evidence="1">Multi-pass membrane protein</topology>
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        <property type="evidence" value="ECO:0007669"/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="De Tomaso A."/>
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                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
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                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
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                <person name="Kobayashi K."/>
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                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
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                <person name="Mochizuki Y."/>
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                <person name="Miura S."/>
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                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">wild type</strain>
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    <reference evidence="1" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
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</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>H2XTH8</accession>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
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                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
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                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
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                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
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                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
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                <person name="Nomoto H."/>
                <person name="Ohta F."/>
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                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
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                <person name="Zhang C."/>
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                <person name="Doggett N."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Richardson P."/>
                <person name="Lucas S."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
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                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">wild type</strain>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">wild type</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Required for correct functioning of the GINS complex, a complex that plays an essential role in the initiation of DNA replication, and progression of DNA replication forks. GINS complex is a core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built.</text>
    </comment>
    <comment type="function">
        <text evidence="6">The GINS complex plays an essential role in the initiation of DNA replication.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the GINS complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the GINS3/PSF3 family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01000811">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2Y306"/>
    <dbReference type="FunCoup" id="H2Y306">
        <property type="interactions" value="314"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000036291"/>
    <dbReference type="Ensembl" id="ENSCINT00000034735.1">
        <property type="protein sequence ID" value="ENSCINP00000036291.1"/>
        <property type="gene ID" value="ENSCING00000022168.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000001622"/>
    <dbReference type="HOGENOM" id="CLU_081646_2_0_1"/>
    <dbReference type="InParanoid" id="H2Y306"/>
    <dbReference type="OMA" id="IYKEGWR"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000811">
        <property type="term" value="C:GINS complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd11713">
        <property type="entry name" value="GINS_A_psf3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21693">
        <property type="entry name" value="GINS_B_Psf3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2050">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021151">
        <property type="entry name" value="GINS_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036224">
        <property type="entry name" value="GINS_bundle-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010492">
        <property type="entry name" value="GINS_Psf3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038437">
        <property type="entry name" value="GINS_Psf3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055221">
        <property type="entry name" value="PSF3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22768">
        <property type="entry name" value="DNA REPLICATION COMPLEX GINS PROTEIN PSF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22768:SF0">
        <property type="entry name" value="DNA REPLICATION COMPLEX GINS PROTEIN PSF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22466">
        <property type="entry name" value="PSF3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05916">
        <property type="entry name" value="Sld5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158573">
        <property type="entry name" value="GINS helical bundle-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160059">
        <property type="entry name" value="PriA/YqbF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA replication complex GINS protein PSF3 N-terminal" evidence="8">
        <location>
            <begin position="17"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="GINS subunit" evidence="7">
        <location>
            <begin position="87"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00045258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF22466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000036291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="206" mass="23342" checksum="47A3FDFDBF31A190" modified="2012-04-18" version="1">MDLLCPPGGGMKQNYLSIDDIMMSSEKIPSTFLVSVKTLGYLDPSTNKKDIEVGSKLDLPLWLALSLAHPRKKFVTVELTKPYKATYREILKADAGVVNLYKLCPYYYALGQQQLSLNHAEGILVARSLILAFSDRFRRIMDLSGNAIGGDNSSLIQQFDEVERRLFALGQKSLQSFQRWEVGESRTLRTSELVSRHRKRKREAVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>H2Y3I1</accession>
    <name>H2Y3I1_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Dynein heavy chain region D6 P-loop domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="H2Y3I1"/>
    <dbReference type="STRING" id="7719.ENSCINP00000036466"/>
    <dbReference type="Ensembl" id="ENSCINT00000033645.1">
        <property type="protein sequence ID" value="ENSCINP00000036466.1"/>
        <property type="gene ID" value="ENSCING00000019172.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154791"/>
    <dbReference type="HOGENOM" id="CLU_071754_0_0_1"/>
    <dbReference type="InParanoid" id="H2Y3I1"/>
    <dbReference type="OMA" id="NRASHNA"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008569">
        <property type="term" value="F:minus-end-directed microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.720:FF:000001">
        <property type="entry name" value="dynein heavy chain 7, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000362">
        <property type="entry name" value="Dynein, axonemal, heavy chain 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.720">
        <property type="entry name" value="Region D6 of dynein motor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041658">
        <property type="entry name" value="AAA_lid_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042219">
        <property type="entry name" value="AAA_lid_11_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026983">
        <property type="entry name" value="DHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004273">
        <property type="entry name" value="Dynein_heavy_D6_P-loop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22878:SF73">
        <property type="entry name" value="DYNEIN AXONEMAL HEAVY CHAIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22878">
        <property type="entry name" value="DYNEIN HEAVY CHAIN 6, AXONEMAL-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18198">
        <property type="entry name" value="AAA_lid_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03028">
        <property type="entry name" value="Dynein_heavy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynein heavy chain region D6 P-loop" evidence="1">
        <location>
            <begin position="43"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain AAA lid" evidence="2">
        <location>
            <begin position="188"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000036466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="276" mass="31495" checksum="3D8EB45AF6F0D131" modified="2012-04-18" version="1">MLRCLRADMVTSAMQDYVAKFMGQRFIEPQTSDLGVVFKESSPVTPLIFVLSTGTDPAADLYKFAEEMRFSKKLSAISLGQGQGPRAEALIRSAMDRGKWVFFQNCHLAPSWMPNLERLIESIDPDKVHRDFRLWLTSMPSNQFPVAILQNGSKMTIEPPRGIKANLLRAYIAQNDDFLNSCTKVSVFKSLLFSLCLFHGVAIERRKFGALGFNIPYEFTTGDLRICISQLKMFLDEYEDIPFKCLTYTAGHINYGGRVTDDWDRRCMMNILSDFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-08-30" modified="2026-01-28" version="116" xmlns="https://uniprot.org/uniprot">
    <accession>Q4H3L8</accession>
    <accession>A0A1W2VNS5</accession>
    <name>Q4H3L8_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="12 13">Ephrin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">Ci-EphrinB</name>
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    <reference evidence="14" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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    <reference evidence="12" key="2">
        <citation type="journal article" date="2003" name="Dev. Genes Evol." volume="213" first="211" last="212">
            <title>Genomewide surveys of developmentally relevant genes in Ciona intestinalis.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Satoh N."/>
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            <dbReference type="PubMed" id="12736827"/>
            <dbReference type="DOI" id="10.1007/s00427-003-0330-z"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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    <reference evidence="12" key="3">
        <citation type="journal article" date="2004" name="Development" volume="131" first="4047" last="4058">
            <title>Gene expression profiles of transcription factors and signaling molecules in the ascidian embryo: towards a comprehensive understanding of gene networks.</title>
            <authorList>
                <person name="Imai K.S."/>
                <person name="Hino K."/>
                <person name="Yagi K."/>
                <person name="Satoh N."/>
                <person name="Satou Y."/>
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            <dbReference type="PubMed" id="15269171"/>
            <dbReference type="DOI" id="10.1242/dev.01270"/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="12" key="4">
        <citation type="submission" date="2005-04" db="EMBL/GenBank/DDBJ databases">
            <title>Expressed genes in Ciona intestinalis.</title>
            <authorList>
                <person name="Satou Y."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="13" key="5">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
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            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="13" key="6">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 7">Belongs to the ephrin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="molecule type" value="mRNA"/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
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                <person name="Chapman J."/>
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                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
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                <person name="Goodstein D.M."/>
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                <person name="Hastings K.E."/>
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                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
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                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
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                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
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                <person name="Makabe K.W."/>
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                <person name="Mochizuki Y."/>
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                <person name="Zhang C."/>
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                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <comment type="catalytic activity">
        <reaction evidence="8 10">
            <text>tRNA(Tyr) + L-tyrosine + ATP = L-tyrosyl-tRNA(Tyr) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10220"/>
            <dbReference type="Rhea" id="RHEA-COMP:9706"/>
            <dbReference type="Rhea" id="RHEA-COMP:9707"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78536"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.1" evidence="1 10"/>
    <dbReference type="EMBL" id="EAAA01002714">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_018812948.1"/>
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        <property type="protein sequence ID" value="ENSCINP00000015337.3"/>
        <property type="gene ID" value="ENSCING00000007475.3"/>
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    <dbReference type="eggNOG" id="KOG2623">
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    <dbReference type="OMA" id="YMMAKDS"/>
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        <property type="component" value="Chromosome 8"/>
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        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
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        <property type="term" value="C:mitochondrion"/>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:tyrosine-tRNA ligase activity"/>
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        <property type="term" value="P:tRNA aminoacylation"/>
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        <property type="term" value="P:tyrosyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000107">
        <property type="entry name" value="Tyrosine--tRNA ligase"/>
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    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
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    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="entry name" value="RNA-binding S4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.240.10">
        <property type="entry name" value="Tyrosyl-Transfer RNA Synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
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    <dbReference type="InterPro" id="IPR002305">
        <property type="entry name" value="aa-tRNA-synth_Ic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
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    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
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    <dbReference type="InterPro" id="IPR002307">
        <property type="entry name" value="Tyr-tRNA-ligase"/>
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    <dbReference type="InterPro" id="IPR024088">
        <property type="entry name" value="Tyr-tRNA-ligase_bac-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00234">
        <property type="entry name" value="tyrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11766:SF0">
        <property type="entry name" value="TYROSINE--TRNA LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11766">
        <property type="entry name" value="TYROSYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00579">
        <property type="entry name" value="tRNA-synt_1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01040">
        <property type="entry name" value="TRNASYNTHTYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55174">
        <property type="entry name" value="Alpha-L RNA-binding motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="4 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00033323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000015337.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="514" mass="58280" checksum="EDA551D4DB2157C4" modified="2017-06-07" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-06-07" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1W5B7S8</accession>
    <accession>H2Y380</accession>
    <name>A0A1W5B7S8_CIOIN</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized LOC100183102</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC100183102</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01000034">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018666829.1">
        <property type="nucleotide sequence ID" value="XM_018811284.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1W5B7S8"/>
    <dbReference type="Ensembl" id="ENSCINT00000034778.1">
        <property type="protein sequence ID" value="ENSCINP00000036365.1"/>
        <property type="gene ID" value="ENSCING00000022851.1"/>
    </dbReference>
    <dbReference type="GeneID" id="100183102"/>
    <dbReference type="KEGG" id="cin:100183102"/>
    <dbReference type="InParanoid" id="A0A1W5B7S8"/>
    <dbReference type="OrthoDB" id="10601369at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="124"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000036365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="227" mass="23665" checksum="A68DEBCA6EA66B40" modified="2017-06-07" version="1">MPISSTNSRLTRELYKPPPRSLEQIRMQEVFNHQIPGSPNPSLKRERHLDGVPKKKTAVVGPVTDNRHAVSNGFAGAADPPLKTSGGVLVNGGVSMWSKSAASGGCLGRMCCICHGYFFTRCCILAFLIGLLLGALITGLVVGLIYAERAEAAGLIAAAAVADDEPVGCLQSPCPSASATCAYSMNDTLLTCNCRPGPTTSTAGINAGGYVCNFFETMTTAVPVTVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>F6PLC9</accession>
    <name>F6PLC9_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">TIMELESS-interacting protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
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                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
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                <person name="Matsumoto J."/>
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                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
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        <scope>IDENTIFICATION</scope>
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                <consortium name="Ensembl"/>
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        <text evidence="7">Plays an important role in the control of DNA replication and the maintenance of replication fork stability.</text>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
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                <person name="Chapman J."/>
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                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
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                <person name="Goodstein D.M."/>
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                <person name="Lemaire P."/>
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                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
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                <person name="Terry A."/>
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                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
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                <person name="Wiley G.B."/>
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                <person name="Lindquist E."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="5">wild type</strain>
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    <reference evidence="5" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
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                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
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                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
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        <citation type="submission" date="2025-09" db="UniProtKB">
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Chapman J."/>
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                <person name="Goodstein D.M."/>
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                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
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                <person name="Lemaire P."/>
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                <person name="Meinertzhagen I.A."/>
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                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
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                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
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                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
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            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">wild type</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="EAAA01001389">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6R658"/>
    <dbReference type="Ensembl" id="ENSCINT00000020052.3">
        <property type="protein sequence ID" value="ENSCINP00000020052.3"/>
        <property type="gene ID" value="ENSCING00000009932.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000066884"/>
    <dbReference type="HOGENOM" id="CLU_1158622_0_0_1"/>
    <dbReference type="InParanoid" id="F6R658"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041298">
        <property type="entry name" value="UBZ3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51907">
        <property type="entry name" value="ZF_UBZ3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="UBZ3-type" evidence="8">
        <location>
            <begin position="206"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="59"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="165"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="77"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="165"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000020052.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="240" mass="26088" checksum="2601FA7ADEBAC288" modified="2012-04-18" version="2">KSFFAHLENKSGDGASCKIEAAYLDSKQNRSTGQLTLQEAVKQNNKSSQRINGLPALIRAGSKPSTSESPSKKIKLAQSQPTSGQKSITGFFGSGKPQLSSVLVQKSKPKSDQKNLGIQTKGAENVPLGNATSVPMGYFRARTQAAAAIQDALKKQRLNTKLKPTSTHDLTTSTNKKQNPTNSHKFFEPQKRATPKANGSLWSSFVQEETYHCDRCSTNVALTEIETHSNFHALQDLNAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>F6RCY1</accession>
    <name>F6RCY1_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA topoisomerase I</fullName>
            <ecNumber evidence="7">5.6.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">DNA topoisomerase 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at the specific target site 5'-[CT]CCTTp site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6 7">
            <text>ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.</text>
            <dbReference type="EC" id="5.6.2.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 6 7">Belongs to the type IB topoisomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.1" evidence="7"/>
    <dbReference type="AlphaFoldDB" id="F6RCY1"/>
    <dbReference type="FunCoup" id="F6RCY1">
        <property type="interactions" value="109"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000006314"/>
    <dbReference type="Ensembl" id="ENSCINT00000006314.3">
        <property type="protein sequence ID" value="ENSCINP00000006314.3"/>
        <property type="gene ID" value="ENSCING00000003086.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000167650"/>
    <dbReference type="HOGENOM" id="CLU_009193_2_0_1"/>
    <dbReference type="InParanoid" id="F6RCY1"/>
    <dbReference type="OMA" id="RKHEYED"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003917">
        <property type="term" value="F:DNA topoisomerase type I (single strand cut, ATP-independent) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00659">
        <property type="entry name" value="Topo_IB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.132.10:FF:000001">
        <property type="entry name" value="DNA topoisomerase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.11.10:FF:000002">
        <property type="entry name" value="DNA topoisomerase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.15.10:FF:000005">
        <property type="entry name" value="DNA topoisomerase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.11.10">
        <property type="entry name" value="DNA Topoisomerase I, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.15.10">
        <property type="entry name" value="Topoisomerase I, Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013030">
        <property type="entry name" value="DNA_topo_DNA_db_N_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001631">
        <property type="entry name" value="TopoI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025834">
        <property type="entry name" value="TopoI_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014711">
        <property type="entry name" value="TopoI_cat_a-hlx-sub_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014727">
        <property type="entry name" value="TopoI_cat_a/b-sub_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013500">
        <property type="entry name" value="TopoI_cat_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008336">
        <property type="entry name" value="TopoI_DNA-bd_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036202">
        <property type="entry name" value="TopoI_DNA-bd_euk_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013499">
        <property type="entry name" value="TopoI_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018521">
        <property type="entry name" value="TopoIB_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051062">
        <property type="entry name" value="Topoisomerase_IB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10290:SF3">
        <property type="entry name" value="DNA TOPOISOMERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10290">
        <property type="entry name" value="DNA TOPOISOMERASE I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14370">
        <property type="entry name" value="Topo_C_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01028">
        <property type="entry name" value="Topoisom_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02919">
        <property type="entry name" value="Topoisom_I_N"/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
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                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
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                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
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                <person name="Sasaki A."/>
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                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
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                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
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                <person name="Richardson P."/>
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                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">wild type</strain>
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    <reference evidence="2" key="3">
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            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="6" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Yamada L."/>
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                <person name="Lemaire P."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">wild type</strain>
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    <reference evidence="6" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="6" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <property type="gene ID" value="ENSCING00000013978.2"/>
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    <dbReference type="KEGG" id="cin:100186957"/>
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        <property type="component" value="Chromosome 10"/>
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        <property type="entry name" value="Macro_OAADPr_deacetylase"/>
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        <property type="entry name" value="Leucine Aminopeptidase, subunit E, domain 1"/>
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        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
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        <property type="entry name" value="Kinase-like_dom_sf"/>
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        <property type="entry name" value="Macro_dom"/>
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        <property type="entry name" value="Macro_dom-like"/>
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        <property type="entry name" value="Prot_kinase_dom"/>
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    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11106">
        <property type="entry name" value="GANGLIOSIDE INDUCED DIFFERENTIATION ASSOCIATED PROTEIN 2-RELATED"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR11106:SF27">
        <property type="entry name" value="MACRO DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01661">
        <property type="entry name" value="Macro"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Pkinase"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Protein kinase-like (PK-like)"/>
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        <property type="entry name" value="MACRO"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
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        <property type="entry name" value="PROTEIN_KINASE_ST"/>
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    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
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        <location>
            <begin position="17"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="Macro" evidence="5">
        <location>
            <begin position="382"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51154"/>
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    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000025445.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="571" mass="64311" checksum="6445D59451EE77DF" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>F6RP84</accession>
    <name>F6RP84_CIOIN</name>
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        <submittedName>
            <fullName evidence="10">Interferon regulatory factor like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">irf-like-3</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
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            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="gene ID" value="ENSCING00000000808.3"/>
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    <dbReference type="HOGENOM" id="CLU_499280_0_0_1"/>
    <dbReference type="InParanoid" id="F6RP84"/>
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    <dbReference type="Proteomes" id="UP000008144">
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00103">
        <property type="entry name" value="IRF"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Interferon_reg_fact_DNA-bd_dom"/>
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        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
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    <dbReference type="PANTHER" id="PTHR11949">
        <property type="entry name" value="INTERFERON REGULATORY FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11949:SF53">
        <property type="entry name" value="IRF TRYPTOPHAN PENTAD REPEAT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00605">
        <property type="entry name" value="IRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00267">
        <property type="entry name" value="INTFRNREGFCT"/>
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    <dbReference type="SMART" id="SM00348">
        <property type="entry name" value="IRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51507">
        <property type="entry name" value="IRF_2"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
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        <location>
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            <end position="123"/>
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        <location>
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        <location>
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    </feature>
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        <location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="512"/>
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        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000001474.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="530" mass="60486" checksum="7C0C85044F577953" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>F6RTY1</accession>
    <name>F6RTY1_CIOIN</name>
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        <submittedName>
            <fullName evidence="5">Splicing factor ESS-2 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100186946</name>
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    <organism evidence="5 6">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ESS2 family.</text>
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    <dbReference type="AlphaFoldDB" id="F6RTY1"/>
    <dbReference type="FunCoup" id="F6RTY1">
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    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000028768"/>
    <dbReference type="Ensembl" id="ENSCINT00000029014.2">
        <property type="protein sequence ID" value="ENSCINP00000028768.2"/>
        <property type="gene ID" value="ENSCING00000016699.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000009387"/>
    <dbReference type="HOGENOM" id="CLU_024820_0_1_1"/>
    <dbReference type="InParanoid" id="F6RTY1"/>
    <dbReference type="OMA" id="AQNDYLD"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
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        <property type="entry name" value="Nuclear_protein_DGCR14_ESS-2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12940">
        <property type="entry name" value="ES-2 PROTEIN - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12940:SF0">
        <property type="entry name" value="SPLICING FACTOR ESS-2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Es2"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="81"/>
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        </location>
    </feature>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="81"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009072"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000028768.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="476" mass="53768" checksum="D8A6C7FC90C3B101" modified="2012-04-18" version="2">MAEIVGVETSLVVATSSKQPRKKFKKVLDEETYTEKVEEIISRDFFPDHERLKDEKEYLEAEEKEDFPRMREIALKYATSGTRMNTFSTPQPSPSTFETPTHSRPPNPSKQTSPMFMKLDDSCNQSNNDDGTKATDDDNQVTLDQFLVKYTSEDNESFERIMDKTDEARRAKHKWLYDAEVRHNEMTNKMLMLEGESPLAITDGTNTRSTVPIHTWKYKNKNHLMYYPEGMTNEDSLFKKPRLIKHENTRFTDHPFKNASDQARIAASESRVVDPERVGHDGKSIVPHESPRVGGYGFVATPSPMPGVTQTPIMTWGEIGGTPARIDGTPRSTPGPAFRFPDESERDRLTHKMVDDNTKKQRAKKQAALKQMKASVLAARGTPGSVMRSERMSSLSPAARRLVNIGTDKALKASYTPTPKRRGGTPLVKTPHIRTPKTLLPNDIQQQSKQATPKGEITDDLLKIPKKVRKSAADFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
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        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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            <taxon>Ascidiacea</taxon>
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    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="F6RWG8"/>
    <dbReference type="Ensembl" id="ENSCINT00000027998.2">
        <property type="protein sequence ID" value="ENSCINP00000027752.2"/>
        <property type="gene ID" value="ENSCING00000015803.2"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1411707_0_0_1"/>
    <dbReference type="InParanoid" id="F6RWG8"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003345921" description="Sushi domain-containing protein" evidence="2">
        <location>
            <begin position="18"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="167"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="183"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000027752.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="193" mass="20624" checksum="9383DDFA3A8C73C2" modified="2011-07-27" version="1">MILRVILLFSVYSLVQGSDTWFNSNSWSCSQTTGLCSGSRSRLCGNAVCTSGIVTEVLTNCPRSNICSGANLVLQVEEACLQSSNDCNAPAIDTVRYTCPQSPGCRVTHRRYCVPTTCIRGTWTPWSDTSSCNATCSGFKTRQRECRDTSTNQLSKLCQGSARETTQTDTASCTPPCPPGTDGRSGGSGCQAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>F6RYM0</accession>
    <accession>A0A1W2W6Z0</accession>
    <name>F6RYM0_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Leukocyte elastase inhibitor-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100176269</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">wild type</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the serpin family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002712">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002125924.4">
        <property type="nucleotide sequence ID" value="XM_002125888.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6RYM0"/>
    <dbReference type="FunCoup" id="F6RYM0">
        <property type="interactions" value="5"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSCINT00000015229.3">
        <property type="protein sequence ID" value="ENSCINP00000015229.3"/>
        <property type="gene ID" value="ENSCING00000007413.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100176269"/>
    <dbReference type="KEGG" id="cin:100176269"/>
    <dbReference type="GeneTree" id="ENSGT00940000163730"/>
    <dbReference type="HOGENOM" id="CLU_023330_0_2_1"/>
    <dbReference type="InParanoid" id="F6RYM0"/>
    <dbReference type="OrthoDB" id="671595at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00172">
        <property type="entry name" value="serpin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.39.10:FF:000030">
        <property type="entry name" value="Serpin 2"/>
        <property type="match status" value="1"/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
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                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
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                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
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                <person name="Zhang C."/>
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                <person name="Doggett N."/>
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    <reference evidence="6" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
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        <text evidence="3">Heterohexamer of two PFD-alpha type and four PFD-beta type subunits. Component of the PAQosome complex which is responsible for the biogenesis of several protein complexes and which consists of R2TP complex members RUVBL1, RUVBL2, RPAP3 and PIH1D1, URI complex members PFDN2, PFDN6, PDRG1, UXT and URI1 as well as ASDURF, POLR2E and DNAAF10/WDR92.</text>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="De Tomaso A."/>
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                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
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                <person name="Hastings K.E."/>
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                <person name="Meinertzhagen I.A."/>
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                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
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                <person name="Suzuki M.M."/>
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                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
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                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
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                <person name="Wiley G.B."/>
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                <person name="Lemaire P."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    </evidence>
    <sequence length="303" mass="34557" checksum="925A83A9427DA148" modified="2012-04-18" version="2">MDEVKSKSCACKGCRTCIVCEGTNGRNVKNQADDLIKDLNEMEYLPSIKMCVSQLKPNGEFVGFSFPGVAVFPDFISENEEKDLVNAINSGQWKDSQSGRRKQDFGPKVNFKRQKVKLASFTGLPKYSKCIDERIKTLKGLESFETVEQCNLEYDPNRGSSIDPHFDDEWLWGERLLSLNLLADSWFTMTTDDSTECQLSVIKTPDVQISRTGVKQIKMDKDDIFHMNDFQVKIPLKRRSLVMLSGDARFKWKHSIQRQDIKSKRMCCTLRELSPEFQTGGKQEHLGRQLLDIASTFSGEVVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F6SFJ3</accession>
    <name>F6SFJ3_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01002665">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6SFJ3"/>
    <dbReference type="STRING" id="7719.ENSCINP00000024421"/>
    <dbReference type="Ensembl" id="ENSCINT00000024667.2">
        <property type="protein sequence ID" value="ENSCINP00000024421.2"/>
        <property type="gene ID" value="ENSCING00000013259.2"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1478256_0_0_1"/>
    <dbReference type="InParanoid" id="F6SFJ3"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="103"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="173"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000024421.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="183" mass="20996" checksum="DDF224301E999195" modified="2012-04-18" version="2">KRPERGYSPPTRTRESRDSRREIVKDSRPARGYTETSYRDYSSPTRRRPRDPSPNQYTASTYTDIKSAAPDRSSYSNSRRQLSPPVQKRSYSTKRSDVGLSRSGRDGGDIREVRDSRDFQSSRNTVTIDRGIPTGTRYQEADVQYGTTDRYAASPSARQLDGHDRVSKRSAHRNSPPTRSRRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>F6SQE1</accession>
    <name>F6SQE1_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">E3 ubiquitin-protein ligase</fullName>
            <ecNumber evidence="9">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">wild type</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 15">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the Deltex family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="9"/>
    <dbReference type="EMBL" id="EAAA01002857">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="EAAA01002858">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="EAAA01002859">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000006511"/>
    <dbReference type="Ensembl" id="ENSCINT00000006511.3">
        <property type="protein sequence ID" value="ENSCINP00000006511.3"/>
        <property type="gene ID" value="ENSCING00000003189.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000169526"/>
    <dbReference type="HOGENOM" id="CLU_420883_0_0_1"/>
    <dbReference type="InParanoid" id="F6SQE1"/>
    <dbReference type="OMA" id="HIMSENN"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007219">
        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd09633">
        <property type="entry name" value="Deltex_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16454">
        <property type="entry name" value="RING-H2_PA-TM-RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.220.10">
        <property type="entry name" value="Leucine Aminopeptidase, subunit E, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039396">
        <property type="entry name" value="Deltex_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039399">
        <property type="entry name" value="Deltex_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039398">
        <property type="entry name" value="Deltex_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002589">
        <property type="entry name" value="Macro_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043472">
        <property type="entry name" value="Macro_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12622">
        <property type="entry name" value="DELTEX-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18102">
        <property type="entry name" value="DTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01661">
        <property type="entry name" value="Macro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00506">
        <property type="entry name" value="A1pp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52949">
        <property type="entry name" value="Macro domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51154">
        <property type="entry name" value="MACRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 9" id="KW-0862">Zinc</keyword>
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    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the complement C6/C7/C8/C9 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_026694203.1">
        <property type="nucleotide sequence ID" value="XM_026838402.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6T090"/>
    <dbReference type="STRING" id="7719.ENSCINP00000017843"/>
    <dbReference type="Ensembl" id="ENSCINT00000017843.3">
        <property type="protein sequence ID" value="ENSCINP00000017843.3"/>
        <property type="gene ID" value="ENSCING00000008759.3"/>
    </dbReference>
    <dbReference type="GeneID" id="113474037"/>
    <dbReference type="GeneTree" id="ENSGT00940000165740"/>
    <dbReference type="HOGENOM" id="CLU_032453_0_0_1"/>
    <dbReference type="InParanoid" id="F6T090"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005579">
        <property type="term" value="C:membrane attack complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006957">
        <property type="term" value="P:complement activation, alternative pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031640">
        <property type="term" value="P:killing of cells of another organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001862">
        <property type="entry name" value="MAC_perforin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020864">
        <property type="entry name" value="MACPF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020863">
        <property type="entry name" value="MACPF_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45742">
        <property type="entry name" value="COMPLEMENT COMPONENT C6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45742:SF8">
        <property type="entry name" value="FLOCCULATION PROTEIN FLO11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01823">
        <property type="entry name" value="MACPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00764">
        <property type="entry name" value="COMPLEMENTC9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00457">
        <property type="entry name" value="MACPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00279">
        <property type="entry name" value="MACPF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51412">
        <property type="entry name" value="MACPF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0179">Complement alternate pathway</keyword>
    <keyword evidence="5" id="KW-0204">Cytolysis</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0391">Immunity</keyword>
    <keyword evidence="8" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="MACPF" evidence="9">
        <location>
            <begin position="43"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000017843.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="421" mass="47267" checksum="81587F5E2D321E05" modified="2012-04-18" version="2">MWEFCFVPLHRGTFGLSCISKRLRCNGDRDCNNFSDEENCLTIHSPCGSKKYKGIPNINRVGAGFDVTKLREESRVMNNEHYNGNCIITRSGKSGKHYRKPANVQFYRYQFQVGHSFTIKSYESSMSYFQDEQNLFKKKMDAGSSFAHLSSLFSNSSGCNSRKTKMVIDFGTNSDSKFFKISATVQVARFRIIRQNLLLSYDFEYRLKQLPEEFDYAKYIQVISDFGTHFYSSGVLGGEYEFLYRYSKKALLESGLTDSEQKHCLEQEAKVSLFGIGSGVDKYGCSTNPLSKKHQGSFTRSATNFVSDVDGGTSAKASALSFTSGKTPNKTDYDAWVKTVKDYPVVVNYMLTPISIAVPVSNYKKRANLERALILYSKEYDNTKCTGKCAHGAAVVVVNKGKLCKCLCPANYGGIDCNVRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>F6TDH1</accession>
    <accession>A0A1W2W1J8</accession>
    <name>F6TDH1_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mitochondrial import inner membrane translocase subunit TIM22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100180734</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8 9">Essential core component of the TIM22 complex, a complex that mediates the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane. In the TIM22 complex, it constitutes the voltage-activated and signal-gated channel. Forms a twin-pore translocase that uses the membrane potential as external driving force in 2 voltage-dependent steps.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the TIM22 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Mitochondrion inner membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the Tim17/Tim22/Tim23 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002121476.2">
        <property type="nucleotide sequence ID" value="XM_002121440.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6TDH1"/>
    <dbReference type="FunCoup" id="F6TDH1">
        <property type="interactions" value="542"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000001694"/>
    <dbReference type="Ensembl" id="ENSCINT00000001694.3">
        <property type="protein sequence ID" value="ENSCINP00000001694.3"/>
        <property type="gene ID" value="ENSCING00000000937.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100180734"/>
    <dbReference type="KEGG" id="cin:100180734"/>
    <dbReference type="GeneTree" id="ENSGT00390000016067"/>
    <dbReference type="HOGENOM" id="CLU_091077_0_0_1"/>
    <dbReference type="InParanoid" id="F6TDH1"/>
    <dbReference type="OMA" id="VNPNMAD"/>
    <dbReference type="OrthoDB" id="75343at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042721">
        <property type="term" value="C:TIM22 mitochondrial import inner membrane insertion complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030943">
        <property type="term" value="F:mitochondrion targeting sequence binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045039">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039175">
        <property type="entry name" value="TIM22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14110">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14110:SF0">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02466">
        <property type="entry name" value="Tim17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0811">Translocation</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="74"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="171"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU367038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000001694.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="193" mass="20786" checksum="AB940E8D2A515453" modified="2011-07-27" version="1">MSTTPKLIDQNVANKEPYTFTTLKYSSVLKHLVGPDKTSTLQPGTYLGGLPTPVPSIYEIRIEKLMQSCLFKSALATVAGAGFGVVFALFTFGVESPSYTDPSKVPTIKETWREMKTRMGSTAKNFATIGAMFSMAHCCIETYRGEADLKNSAYSGFVTGGILGLRGGIKAGFLGGAGFAAFSTAIDYYFLHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>F6TN12</accession>
    <name>F6TN12_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Sodium-coupled monocarboxylate transporter 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
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                <person name="Martinez D."/>
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                <person name="Putnam N."/>
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                <person name="Terry A."/>
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                <person name="Wang H.G."/>
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                <person name="Azumi K."/>
                <person name="Boore J."/>
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                <person name="Richardson P."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="22"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000013626.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="254" mass="28839" checksum="858AEDA28B691232" modified="2011-07-27" version="1">MSKIFVNGVELFYQKFGTGEHVVLLLCGGLGFGNIHYKPQIEGFDLEKFTLISWDPRGYGQSRPPKRVFPKDFYYIDADDAIALMEKLGYESYSLLGWSDGAILAAIIGANKSEKVRKIVKWGGNAFCTPDMMVVYQGLHDGSIWSEDAFGPLLKYYGKEYFDCILKAWCASFKTMDKDSEGNVNICSEYLSDVKAPTLIIHGMKDGLVVKMHADFFLEKIKDSKLVVFDEGDHHLHLQFPEKFNRLAQEFLLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>F6U637</accession>
    <name>F6U637_CIOIN</name>
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        <recommendedName>
            <fullName evidence="12">VWFA domain-containing protein</fullName>
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    <organism evidence="10 11">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">wild type</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ITIH family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001790">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6U637"/>
    <dbReference type="STRING" id="7719.ENSCINP00000023266"/>
    <dbReference type="Ensembl" id="ENSCINT00000023512.2">
        <property type="protein sequence ID" value="ENSCINP00000023266.2"/>
        <property type="gene ID" value="ENSCING00000008945.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000167412"/>
    <dbReference type="HOGENOM" id="CLU_008101_2_0_1"/>
    <dbReference type="InParanoid" id="F6U637"/>
    <dbReference type="OMA" id="IRGMEYP"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030212">
        <property type="term" value="P:hyaluronan metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010600">
        <property type="entry name" value="ITI_HC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050934">
        <property type="entry name" value="ITIH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013694">
        <property type="entry name" value="VIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10338">
        <property type="entry name" value="INTER-ALPHA-TRYPSIN INHIBITOR HEAVY CHAIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10338:SF108">
        <property type="entry name" value="INTER-ALPHA-TRYPSIN INHIBITOR HEAVY CHAIN H4-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06668">
        <property type="entry name" value="ITI_HC_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08487">
        <property type="entry name" value="VIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13768">
        <property type="entry name" value="VWA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00609">
        <property type="entry name" value="VIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51468">
        <property type="entry name" value="VIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0722">Serine protease inhibitor</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="domain" description="VIT" evidence="9">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="8">
        <location>
            <begin position="234"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000023266.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="689" mass="76901" checksum="EBDE5F5A6DD27C84" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>F6U696</accession>
    <accession>A0A1W2W6G6</accession>
    <name>F6U696_CIOIN</name>
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        <recommendedName>
            <fullName evidence="4">Sugar transporter SWEET1</fullName>
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        <alternativeName>
            <fullName evidence="13">Solute carrier family 50 member 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC100185787</name>
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    <organism evidence="16 17">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">wild type</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Mediates sugar transport across membranes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SWEET sugar transporter family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01000584">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002125273.1">
        <property type="nucleotide sequence ID" value="XM_002125237.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6U696"/>
    <dbReference type="STRING" id="7719.ENSCINP00000011286"/>
    <dbReference type="TCDB" id="2.A.123.1.11">
        <property type="family name" value="the sweet, pq-loop, saliva, mtn3 (sweet) family"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSCINT00000011286.3">
        <property type="protein sequence ID" value="ENSCINP00000011286.3"/>
        <property type="gene ID" value="ENSCING00000005468.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100185787"/>
    <dbReference type="KEGG" id="cin:100185787"/>
    <dbReference type="GeneTree" id="ENSGT00390000007801"/>
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        <source>
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    <sequence length="215" mass="23595" checksum="36E28963DDFE9AFE" modified="2012-04-18" version="2">MAEFWISFFSNGCIAVTIIMFATGIPQCMEMMKKKTTKNIPFLPYLITNVNAIGWIIYGKMTVNFTVVFVNTIGAGLQTLYMAVYIFFAADKSKPLVQSSVCGGAAAITWYIITQFANVIDAINVTGIICCTVTIFMFASPLAEINTVIANKSTATISLPLTVTASLCSAMWTMFGLVLHDNFIIIPNVLGFFAAFSRFYLFYKYPSSPGLPHSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>F6UDU8</accession>
    <name>F6UDU8_CIOIN</name>
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        <submittedName>
            <fullName evidence="4">Intraflagellar transport protein 88 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100181294</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
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            <taxon>Phlebobranchia</taxon>
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            <taxon>Ciona</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">wild type</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="STRING" id="7719.ENSCINP00000005526"/>
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        <property type="protein sequence ID" value="ENSCINP00000005526.3"/>
        <property type="gene ID" value="ENSCING00000002712.4"/>
    </dbReference>
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    <dbReference type="HOGENOM" id="CLU_010738_1_0_1"/>
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        <property type="component" value="Chromosome 1"/>
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        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="project" value="GO_Central"/>
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        <property type="term" value="C:non-motile cilium"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019894">
        <property type="term" value="F:kinesin binding"/>
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        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0042073">
        <property type="term" value="P:intraciliary transport"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="P:non-motile cilium assembly"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Intraflagellar transport 88 homolog (Chlamydomonas)"/>
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        <property type="entry name" value="INTRAFLAGELLAR TRANSPORT PROTEIN 88 HOMOLOG"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="INTRAFLAGELLAR TRANSPORT PROTEIN 88 HOMOLOG"/>
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        <property type="entry name" value="TPR_16"/>
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    </dbReference>
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        <property type="match status" value="8"/>
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            <begin position="278"/>
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            <begin position="629"/>
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        <location>
            <begin position="727"/>
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        <location>
            <begin position="137"/>
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        <location>
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        <location>
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        <location>
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    </feature>
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        <location>
            <begin position="815"/>
            <end position="830"/>
        </location>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000005526.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="830" mass="92036" checksum="D7DDCD01620FDBAB" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>F6UIA2</accession>
    <name>F6UIA2_CIOIN</name>
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        <recommendedName>
            <fullName evidence="8">Protein arginine N-methyltransferase</fullName>
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    <organism evidence="11 12">
        <name type="scientific">Ciona intestinalis</name>
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        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles. Specifically mediates the symmetric dimethylation of histone H4 'Arg-3' to form H4R3me2s. Plays a role in gene imprinting by being recruited by CTCFL at the H19 imprinted control region (ICR) and methylating histone H4 to form H4R3me2s, possibly leading to recruit DNA methyltransferases at these sites. May also play a role in embryonic stem cell (ESC) pluripotency. Also able to mediate the arginine methylation of histone H2A and myelin basic protein (MBP) in vitro; the relevance of such results is however unclear in vivo.</text>
    </comment>
    <comment type="function">
        <text evidence="5">Essential arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA). Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins SmD1 and SmD3.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-arginyl-[protein] + S-adenosyl-L-methionine = N(omega)-methyl-L-arginyl-[protein] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48100"/>
            <dbReference type="Rhea" id="RHEA-COMP:10532"/>
            <dbReference type="Rhea" id="RHEA-COMP:11990"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29965"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65280"/>
            <dbReference type="EC" id="2.1.1.321"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N-methyltransferase family. PRMT7 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="8"/>
    <dbReference type="AlphaFoldDB" id="F6UIA2"/>
    <dbReference type="FunCoup" id="F6UIA2">
        <property type="interactions" value="628"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000010866"/>
    <dbReference type="Ensembl" id="ENSCINT00000010866.3">
        <property type="protein sequence ID" value="ENSCINP00000010866.3"/>
        <property type="gene ID" value="ENSCING00000005280.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156879"/>
    <dbReference type="HOGENOM" id="CLU_015180_0_0_1"/>
    <dbReference type="InParanoid" id="F6UIA2"/>
    <dbReference type="OMA" id="CHHDEYS"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042054">
        <property type="term" value="F:histone methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016274">
        <property type="term" value="F:protein-arginine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035241">
        <property type="term" value="F:protein-arginine omega-N monomethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.160.11:FF:000010">
        <property type="entry name" value="Protein arginine N-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.160.11:FF:000014">
        <property type="entry name" value="Protein arginine N-methyltransferase 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000070">
        <property type="entry name" value="Protein arginine N-methyltransferase 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000071">
        <property type="entry name" value="Protein arginine N-methyltransferase 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.160.11">
        <property type="entry name" value="Hnrnp arginine n-methyltransferase1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025799">
        <property type="entry name" value="Arg_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014644">
        <property type="entry name" value="MeTrfase_PRMT7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055135">
        <property type="entry name" value="PRMT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11006">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11006:SF4">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06325">
        <property type="entry name" value="PrmA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22528">
        <property type="entry name" value="PRMT_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036946">
        <property type="entry name" value="Arg_N-mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51678">
        <property type="entry name" value="SAM_MT_PRMT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein arginine N-methyltransferase" evidence="10">
        <location>
            <begin position="180"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="Protein arginine N-methyltransferase" evidence="10">
        <location>
            <begin position="511"/>
            <end position="691"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR036946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF22528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000010866.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="696" mass="79442" checksum="EEACBAEF1F3A4CBF" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>F6UJL9</accession>
    <name>F6UJL9_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">Tartrate-resistant acid phosphatase type 5</fullName>
            <ecNumber evidence="2 6">3.1.3.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">wild type</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>a phosphate monoester + H2O = an alcohol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15017"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:67140"/>
            <dbReference type="EC" id="3.1.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="7">Binds 2 iron ions per subunit.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.2" evidence="2 6"/>
    <dbReference type="EMBL" id="EAAA01002720">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6UJL9"/>
    <dbReference type="STRING" id="7719.ENSCINP00000015403"/>
    <dbReference type="Ensembl" id="ENSCINT00000015403.3">
        <property type="protein sequence ID" value="ENSCINP00000015403.3"/>
        <property type="gene ID" value="ENSCING00000007515.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000016735"/>
    <dbReference type="HOGENOM" id="CLU_043332_1_0_1"/>
    <dbReference type="InParanoid" id="F6UJL9"/>
    <dbReference type="OMA" id="NVEMYIN"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003993">
        <property type="term" value="F:acid phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008199">
        <property type="term" value="F:ferric iron binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045453">
        <property type="term" value="P:bone resorption"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07378">
        <property type="entry name" value="MPP_ACP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000062">
        <property type="entry name" value="Tartrate-resistant acid phosphatase type 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024927">
        <property type="entry name" value="Acid_PPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051558">
        <property type="entry name" value="Metallophosphoesterase_PAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10161">
        <property type="entry name" value="TARTRATE-RESISTANT ACID PHOSPHATASE TYPE 5"/>
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</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>F6UVK0</accession>
    <name>F6UVK0_CIOIN</name>
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        <recommendedName>
            <fullName evidence="5">Macoilin</fullName>
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        <alternativeName>
            <fullName evidence="13">Transmembrane protein 57</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Tunicata</taxon>
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            <taxon>Phlebobranchia</taxon>
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            <taxon>Ciona</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Plays a role in the regulation of neuronal activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Rough endoplasmic reticulum membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the macoilin family.</text>
    </comment>
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        <property type="interactions" value="190"/>
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    <dbReference type="Ensembl" id="ENSCINT00000027100.2">
        <property type="protein sequence ID" value="ENSCINP00000026854.2"/>
        <property type="gene ID" value="ENSCING00000014982.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000016613"/>
    <dbReference type="HOGENOM" id="CLU_012823_1_0_1"/>
    <dbReference type="InParanoid" id="F6UVK0"/>
    <dbReference type="OMA" id="KQRTACE"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="C:rough endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0023041">
        <property type="term" value="P:neuronal signal transduction"/>
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        <property type="project" value="GO_Central"/>
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        <property type="entry name" value="Macoilin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47464">
        <property type="entry name" value="MACOILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47464:SF2">
        <property type="entry name" value="MACOILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09726">
        <property type="entry name" value="Macoilin"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
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    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 15" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="21"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="70"/>
            <end position="87"/>
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        <location>
            <begin position="214"/>
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        <location>
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        </location>
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            <begin position="223"/>
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            <begin position="246"/>
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        </location>
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            <begin position="261"/>
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        </location>
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        <source>
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        <source>
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    </evidence>
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        <source>
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        </source>
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        <source>
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        <source>
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00031129"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000026854.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="728" mass="82531" checksum="FA890A7464817FB7" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>F6V4Q7</accession>
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        <recommendedName>
            <fullName evidence="4">Ig-like domain-containing protein</fullName>
        </recommendedName>
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        <name type="scientific">Ciona intestinalis</name>
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        <name type="synonym">Ascidia intestinalis</name>
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    <reference evidence="6" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">wild type</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="entry name" value="IG-LIKE DOMAIN-CONTAINING PROTEIN"/>
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        <property type="match status" value="1"/>
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    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003343583" description="Ig-like domain-containing protein" evidence="3">
        <location>
            <begin position="25"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="68"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000024367.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="153" mass="16862" checksum="7B2603D8AEAE0C29" modified="2012-04-18" version="2">MNCARSVGCCLLICFLAFCGDCSASKTSQRKEVLRRIFRSVDLTPSISTTPSASSIDQPITPAPTEDPDMELNLMEDMVNQTLQSGGKAIFRCKVEGTPTDAVIFTWYKDGTELVDDPRFNIRTKSWGSLLKISDVYTADSGQYRCEASIRTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>F6VIA3</accession>
    <name>F6VIA3_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Kinesin-like KIF1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">wild type</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01001773">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6VIA3"/>
    <dbReference type="STRING" id="7719.ENSCINP00000023207"/>
    <dbReference type="Ensembl" id="ENSCINT00000023453.2">
        <property type="protein sequence ID" value="ENSCINP00000023207.2"/>
        <property type="gene ID" value="ENSCING00000009044.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000168697"/>
    <dbReference type="HOGENOM" id="CLU_026051_0_0_1"/>
    <dbReference type="InParanoid" id="F6VIA3"/>
    <dbReference type="OMA" id="FINISPC"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022140">
        <property type="entry name" value="Kinesin-like_KIF1-typ"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12423">
        <property type="entry name" value="KIF1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin-like KIF1-type" evidence="3">
        <location>
            <begin position="74"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="389"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="299"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="413"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="435"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="464"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000023207.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="484" mass="55282" checksum="A2DFF4CD1FBECFC9" modified="2012-04-18" version="2">MSKDQQRAQEQVLELLPMVSEVNAVSEELNKYRHFELVLISGGYQEDKDKGTKVMVQVKNLLNNNIWMWERGKFMNRRYIIQELYQQYIDGDDSYKNIEQDKDPFFDPPEDVMLGTANVFLQSLSYALDFDDKLAITDYKGQEEGMLTVAVTPCLPNGRSLDEESFIEDPSDLLGKPYHFKITIRNCEINNSRYSKGVYVRHKTLGDKDPIKTKVVEDTLSPEFNYSRIITIPSVNQEHLDYFESQSITFFVHGCQVDTPPVASVKKLTTKELREMDGQMTAPMLRRRNTLVASNMAVDSQLKSEIHTLQRKYDRLEKKEKRIQELCRQWSDKSPEEQDFEKFMNSIKAVAFSSGNKLKSRVQLVNHRDSDAFSVYSFEVTPEMKFISGLSEDPIQEEEEVSNESGSRRGSSPKKDHDTTSKDAKQSNGSPKRKSQSDKDSPQKHSNGKEKLVQATKPAASENGRPSPTSSGNSAKESKTCIIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>F6VZ27</accession>
    <name>F6VZ27_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">MRH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Tunicata</taxon>
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            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">wild type</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01002706">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6VZ27"/>
    <dbReference type="STRING" id="7719.ENSCINP00000015124"/>
    <dbReference type="Ensembl" id="ENSCINT00000015124.3">
        <property type="protein sequence ID" value="ENSCINP00000015124.3"/>
        <property type="gene ID" value="ENSCING00000007367.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00510000047770"/>
    <dbReference type="HOGENOM" id="CLU_094443_1_0_1"/>
    <dbReference type="InParanoid" id="F6VZ27"/>
    <dbReference type="OMA" id="NARHEFT"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.130.10">
        <property type="entry name" value="Mannose-6-phosphate receptor binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039794">
        <property type="entry name" value="Gtb1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009011">
        <property type="entry name" value="Man6P_isomerase_rcpt-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044865">
        <property type="entry name" value="MRH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036607">
        <property type="entry name" value="PRKCSH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12630:SF1">
        <property type="entry name" value="GLUCOSIDASE 2 SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12630">
        <property type="entry name" value="N-LINKED OLIGOSACCHARIDE PROCESSING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13015">
        <property type="entry name" value="PRKCSH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50911">
        <property type="entry name" value="Mannose 6-phosphate receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51914">
        <property type="entry name" value="MRH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="MRH" evidence="3">
        <location>
            <begin position="14"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000015124.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="126" mass="14367" checksum="91641245666F8844" modified="2012-04-18" version="2">MDFGPDESYQALQFQCYELQTMEYTYKLCPFDKTSQSPKNGGTETNLGRWGSWSGGNDDKYSKMKYDNGLTCWNGPARSTEVRIKCGVEHKLLSVDEPSRCAYTFEFATPCACKQTQQDSQPRDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>F6WBI7</accession>
    <name>F6WBI7_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Succinate-semialdehyde dehydrogenase</fullName>
            <ecNumber evidence="7">1.2.1.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100180018</name>
    </gene>
    <organism evidence="9 10">
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
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                <person name="Doyle S."/>
                <person name="Francino P."/>
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                <person name="Hayashi H."/>
                <person name="Hino K."/>
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                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
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                <consortium name="Ensembl"/>
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            <text>succinate semialdehyde + NAD(+) + H2O = succinate + NADH + 2 H(+)</text>
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    <dbReference type="GO" id="GO:0042645">
        <property type="term" value="C:mitochondrial nucleoid"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070987">
        <property type="term" value="P:error-free translesion synthesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="Gene3D" id="2.60.40.1470">
        <property type="entry name" value="ApaG domain"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="2.30.30.390">
        <property type="entry name" value="Hemimethylated DNA-binding domain"/>
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        <property type="entry name" value="F-BOX ONLY PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14289:SF16">
        <property type="entry name" value="POLYMERASE DELTA-INTERACTING PROTEIN 2"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF04379">
        <property type="entry name" value="DUF525"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="YccV-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00992">
        <property type="entry name" value="YccV-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ApaG-like"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="APAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ApaG" evidence="1">
        <location>
            <begin position="249"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000016033.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="376" mass="43114" checksum="04427A7FFCD6CFBF" modified="2012-04-18" version="2">MSKSKSLLCIRNINCLNGKDSLFNKFCSNDLPFVSRLQYRRYLCFVHKRKRTLQSTFLSTRILKRGHVTKQVKLDHVGILENLKSDGNYSPGQMFLHRVFGYRGVILYPWTAHIYEKNSEATPEPNKENEKSEDSKPVPVTETFYQVLVDARDSPHVAVQSEAVTFLTNSQPGENNHSSLYTIPGIDYVSQHDILPYTSVEPLPMSHELFPKFLEAEQTTNGTVLSTTETLASWQKKNHFCLELTKVHIETTENIRVTAIPFYLGAKESRNSLEYWWRYCIRIENLGETGAHLRERHWRIISNGSVKTVRGRGVIGREPMLNKSQPAFQYSSHVSLQSSSGHMWGIFRLEQEDGKQFDARIPAFALDGNKTTKTEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>F6WUP8</accession>
    <name>F6WUP8_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DOCKER domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">wild type</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DOCK family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002063">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6WUP8"/>
    <dbReference type="STRING" id="7719.ENSCINP00000019638"/>
    <dbReference type="Ensembl" id="ENSCINT00000019638.3">
        <property type="protein sequence ID" value="ENSCINP00000019638.3"/>
        <property type="gene ID" value="ENSCING00000009657.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154974"/>
    <dbReference type="HOGENOM" id="CLU_674321_0_0_1"/>
    <dbReference type="InParanoid" id="F6WUP8"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.740:FF:000004">
        <property type="entry name" value="Dedicator of cytokinesis protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026791">
        <property type="entry name" value="DOCK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043162">
        <property type="entry name" value="DOCK_C_lobe_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027357">
        <property type="entry name" value="DOCKER_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046770">
        <property type="entry name" value="DOCKER_Lobe_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046773">
        <property type="entry name" value="DOCKER_Lobe_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45653">
        <property type="entry name" value="DEDICATOR OF CYTOKINESIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45653:SF10">
        <property type="entry name" value="MYOBLAST CITY, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20422">
        <property type="entry name" value="DHR-2_Lobe_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20421">
        <property type="entry name" value="DHR-2_Lobe_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51651">
        <property type="entry name" value="DOCKER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DOCKER" evidence="3">
        <location>
            <begin position="1"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="243"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="263"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="289"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="306"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="362"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000019638.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="408" mass="46311" checksum="4F0AB957DDA56A95" modified="2012-04-18" version="2">MEIPSHFKGKTVDDQILSFYKVNELQRFQYSKPFRKGPKDKDNEFASMWIERYTYTTRYKLPGILRWFEIEDTSKREISPLENAMDAMETANEEIRQMILKYYKNPEFPINPLSMKLNGVVDPRVMGGFTNYEKAFFTETYMKENSDDLEKVSRLKDLIALQVPLLAEGVRLHGELAPSQLQPFHQNMESSFQELKTYVEKQYGKRPVFELGRPVRRVSPQRNVMSMKQPRPESILSMQSNTSDHYHLTDGTPPEVPAKRPTSRLSNVISRSTSLAAPADKPGKRGSRTSEFFVNNFGSTNSLDKSVSSISSTGSGSGVVELREELTPQRPRRPKSQVGIPGVLGSKPVSGHKSPPPLPVKHQTDQHHEEKSSPVTRKPPPPLPVKQHDSSEKTNLLPPKKPGRSPRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>F6WWY2</accession>
    <name>F6WWY2_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="8">wild type</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
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                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
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                <person name="Haga S."/>
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                <person name="Kobayashi M."/>
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                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
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                <person name="Shoguchi E."/>
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                <person name="Suzuki M.M."/>
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                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="12" key="2">
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        <scope>IDENTIFICATION</scope>
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    <comment type="subcellular location">
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        <property type="gene ID" value="ENSCING00000015947.2"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="term" value="C:membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:GTPase activator activity"/>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
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        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
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    </dbReference>
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        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
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        <property type="entry name" value="DAG/PE-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051854">
        <property type="entry name" value="Rho-type_GAP"/>
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        <property type="entry name" value="RhoGAP_dom"/>
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        <property type="entry name" value="CHIMERIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RHO GTPASE ACTIVATING PROTEIN AT 5A, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00008">
        <property type="entry name" value="DAGPEDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="1"/>
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    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="10">
        <location>
            <begin position="5"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="11">
        <location>
            <begin position="68"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00073081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00076015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00077047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000027922.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="262" mass="29779" checksum="1605A21E2B8BC80F" modified="2012-04-18" version="2">QYEKEHHYKSHTYRGLQWCEYCGNFMWGIIQQGVQCVDCGLNVHKQCAKLVPNDCQPALKHIHHVFGVDLTTLVKLHSTQRPLVVDMCIAEIEKRGMDSEGIYRIPGFHDDVIELKAAFDQLGTEVNMTAYEDVNTIAGALKLYLRELPVPLLPYRLYSRFINAAKISHENGKLDAIRMALSATPGAHFETIKYLIQHLGRVSERSNENQMSAHNLGIVFGPTLLRAPENDVNQRYNLNDTQFQQEAIASMIGYRQHLFGLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>F6X9K9</accession>
    <name>F6X9K9_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">wild type</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01001645">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6X9K9"/>
    <dbReference type="STRING" id="7719.ENSCINP00000010210"/>
    <dbReference type="Ensembl" id="ENSCINT00000010210.3">
        <property type="protein sequence ID" value="ENSCINP00000010210.3"/>
        <property type="gene ID" value="ENSCING00000004965.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162700"/>
    <dbReference type="HOGENOM" id="CLU_975127_0_0_1"/>
    <dbReference type="InParanoid" id="F6X9K9"/>
    <dbReference type="OMA" id="DISHSAY"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000032">
        <property type="entry name" value="palladin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10075">
        <property type="entry name" value="BASIGIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10075:SF25">
        <property type="entry name" value="PROTEIN TURTLE HOMOLOG A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003345147" description="Ig-like domain-containing protein" evidence="3">
        <location>
            <begin position="23"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="31"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="138"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="229"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000010210.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="323" mass="35864" checksum="EE8326104C01A42C" modified="2012-04-18" version="2">MNGQSQALLVGTIFWILLSVETRGLISVSVGATALLPCPSSRNDQDLPHVIEWLRQDRPVPVFIKIGKYPPHVNPHFKGRMSLLSNTATLRIQKVKLEDEGWYECRAYYFKHAVESSNGTLVHPSNGTLTKLYVTAPPAFKSTPPPSTVARVRESLLLQCRVVGKPPPDVTWRKDGWLLQNGENGIKINGGKLLVRSLTTSHRGNYECQGRNPQGQVEHTTSVFVQAPPEITVSPKDLDAKEEQDAFFYCKAEGYPADISYSWFFNGLEVGSCPQLRGRFRTFNDGTFEIESVSKEDTGNISCEAANDFGIAPRVTARLVVQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>F6YJM6</accession>
    <name>F6YJM6_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">B(0,+)-type amino acid transporter 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100177912</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
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                <person name="Wiley G.B."/>
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                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">wild type</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
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        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TBP family.</text>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <reference evidence="18" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">wild type</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>(6R)-10-formyltetrahydrofolate + NADP(+) + H2O = (6S)-5,6,7,8-tetrahydrofolate + CO2 + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10180"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:195366"/>
            <dbReference type="EC" id="1.5.1.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:10181"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">In the C-terminal section; belongs to the aldehyde dehydrogenase family. ALDH1L subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">In the N-terminal section; belongs to the GART family.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.6" evidence="9"/>
    <dbReference type="EMBL" id="EAAA01002614">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002130073.3">
        <property type="nucleotide sequence ID" value="XM_002130037.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6Z6Y7"/>
    <dbReference type="FunCoup" id="F6Z6Y7">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000016529"/>
    <dbReference type="Ensembl" id="ENSCINT00000016529.3">
        <property type="protein sequence ID" value="ENSCINP00000016529.3"/>
        <property type="gene ID" value="ENSCING00000008076.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100184767"/>
    <dbReference type="KEGG" id="cin:100184767"/>
    <dbReference type="GeneTree" id="ENSGT00940000169311"/>
    <dbReference type="HOGENOM" id="CLU_014974_0_0_1"/>
    <dbReference type="InParanoid" id="F6Z6Y7"/>
    <dbReference type="OMA" id="NEQVFMA"/>
    <dbReference type="OrthoDB" id="310895at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004029">
        <property type="term" value="F:aldehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016155">
        <property type="term" value="F:formyltetrahydrofolate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009258">
        <property type="term" value="P:10-formyltetrahydrofolate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07140">
        <property type="entry name" value="ALDH_F1L_FTFDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08703">
        <property type="entry name" value="FDH_Hydrolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08647">
        <property type="entry name" value="FMT_core_FDH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1200.10:FF:000002">
        <property type="entry name" value="10-formyltetrahydrofolate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.25.10:FF:000002">
        <property type="entry name" value="10-formyltetrahydrofolate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.170:FF:000002">
        <property type="entry name" value="10-formyltetrahydrofolate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000050">
        <property type="entry name" value="Aldehyde dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000026">
        <property type="entry name" value="Aldehyde dehydrogenase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000008">
        <property type="entry name" value="Cytosolic 10-formyltetrahydrofolate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.25.10">
        <property type="entry name" value="Formyl transferase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.170">
        <property type="entry name" value="Formyl transferase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011407">
        <property type="entry name" value="10_FTHF_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005793">
        <property type="entry name" value="Formyl_trans_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037022">
        <property type="entry name" value="Formyl_trans_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002376">
        <property type="entry name" value="Formyl_transf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036477">
        <property type="entry name" value="Formyl_transf_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011034">
        <property type="entry name" value="Formyl_transferase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001555">
        <property type="entry name" value="GART_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11699">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02911">
        <property type="entry name" value="Formyl_trans_C"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00551">
        <property type="entry name" value="Formyl_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036489">
        <property type="entry name" value="10-FTHFDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50486">
        <property type="entry name" value="FMT C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53328">
        <property type="entry name" value="Formyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00373">
        <property type="entry name" value="GART"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9 12" id="KW-0521">NADP</keyword>
    <keyword evidence="5 9" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="7 9 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carrier" evidence="16">
        <location>
            <begin position="340"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="10">
        <location>
            <position position="129"/>
        </location>
    </feature>
    <feature type="active site" evidence="14">
        <location>
            <position position="692"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10">
        <location>
            <position position="692"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="10">
        <location>
            <position position="726"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="111"/>
            <end position="113"/>
        </location>
        <ligand>
            <name>(6R)-10-formyltetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:195366"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>(6R)-10-formyltetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:195366"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="590"/>
            <end position="592"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="616"/>
            <end position="619"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="649"/>
            <end position="654"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="669"/>
            <end position="670"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="776"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="823"/>
            <end position="825"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="site" description="Essential for catalytic activity" evidence="13">
        <location>
            <position position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR036489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR036489-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR036489-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR036489-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR036489-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000016529.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="921" mass="101183" checksum="CCBDF924248E8022" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>F6ZL23</accession>
    <accession>A0A1W2WEB5</accession>
    <name>F6ZL23_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein jagunal homolog 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100186310</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Wiley G.B."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">wild type</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="13">Largely monomeric, dimerizes on the Holliday junction and the first nick occurs upon dimerization at the junction.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the XPG/RAD2 endonuclease family. GEN subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001478">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_002124475.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6ZYE8"/>
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        <property type="protein sequence ID" value="ENSCINP00000017669.3"/>
        <property type="gene ID" value="ENSCING00000008651.3"/>
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    <dbReference type="GeneID" id="100182716"/>
    <dbReference type="KEGG" id="cin:100182716"/>
    <dbReference type="GeneTree" id="ENSGT00940000159266"/>
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    <dbReference type="InParanoid" id="F6ZYE8"/>
    <dbReference type="OMA" id="TKHEGHQ"/>
    <dbReference type="OrthoDB" id="2959108at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 2"/>
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    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:5'-flap endonuclease activity"/>
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        <property type="term" value="F:crossover junction DNA endonuclease activity"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="F:four-way junction DNA binding"/>
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        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="PIN_GEN1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="flap endonuclease GEN homolog 1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Flap endonuclease GEN-like 1"/>
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        <property type="entry name" value="5-3_exonuclease_C_sf"/>
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        <property type="entry name" value="GEN_chromo"/>
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        <property type="entry name" value="HhH2"/>
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        <property type="entry name" value="PIN-like_dom_sf"/>
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        <property type="entry name" value="XPG-I_dom"/>
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    <dbReference type="InterPro" id="IPR006084">
        <property type="entry name" value="XPG/Rad2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006085">
        <property type="entry name" value="XPG_DNA_repair_N"/>
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        <property type="entry name" value="FLAP ENDONUCLEASE FAMILY MEMBER"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081:SF70">
        <property type="entry name" value="FLAP ENDONUCLEASE GEN HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Chromo_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="XPG_N"/>
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    <dbReference type="PRINTS" id="PR00853">
        <property type="entry name" value="XPGRADSUPER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00279">
        <property type="entry name" value="HhH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00484">
        <property type="entry name" value="XPGI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00485">
        <property type="entry name" value="XPGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47807">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
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    <feature type="domain" description="XPG-I" evidence="16">
        <location>
            <begin position="122"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="490"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="656"/>
            <end position="690"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="496"/>
            <end position="512"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="656"/>
            <end position="677"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00038112"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00063132"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00070188"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="SMART" id="SM00484"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00485"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000017669.3"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>F7ANK8</accession>
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        <submittedName>
            <fullName evidence="3">BTB/POZ domain-containing protein kctd15-like</fullName>
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    <gene>
        <name evidence="3" type="primary">LOC100180969</name>
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    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
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            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="F7ANK8"/>
    <dbReference type="STRING" id="7719.ENSCINP00000027274"/>
    <dbReference type="Ensembl" id="ENSCINT00000027520.2">
        <property type="protein sequence ID" value="ENSCINP00000027274.2"/>
        <property type="gene ID" value="ENSCING00000006304.3"/>
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    <dbReference type="GeneTree" id="ENSGT00940000170674"/>
    <dbReference type="HOGENOM" id="CLU_061268_0_0_1"/>
    <dbReference type="InParanoid" id="F7ANK8"/>
    <dbReference type="OMA" id="FCDCIAV"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18361">
        <property type="entry name" value="BTB_POZ_KCTD1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
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    <dbReference type="InterPro" id="IPR048595">
        <property type="entry name" value="KCTD1-15-like_C"/>
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    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14499:SF67">
        <property type="entry name" value="BTB_POZ DOMAIN-CONTAINING PROTEIN TIWAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14499">
        <property type="entry name" value="POTASSIUM CHANNEL TETRAMERIZATION DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF20871">
        <property type="entry name" value="KCTD1-15_CTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="276"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
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            <end position="46"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="280"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000027274.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="297" mass="33374" checksum="9D74A6134CF3C7EA" modified="2012-04-18" version="2">MPEHDGRRITKRTDKPPTSNDTRPPRTPPDIRYNQPNIRTTPNHEQPTLVGVPKAAVPSRYAAPVHIDVGGHIYTSSLETLTKFPDSKLGRLFSGAEPIVLDTLKQHYFIDRDGNMFRHILNFLRTSNLALPDDFHELDQLLQEARFFELKLMVGQLEQFKESRRVSKTHKDCVVIRTVPDLGERICLGGNKSIIASIFPEVYDVITSADKCTWNQDPTHVIRFPINAFCQINSIKVVLETFLSSGFSILAASGGGSESSSHFTEWVVARPRHNHDDVINTTSSTSTNKNSKLNSKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>F7APZ8</accession>
    <accession>A0A1W2WB39</accession>
    <name>F7APZ8_CIOIN</name>
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        <recommendedName>
            <fullName evidence="8">Glycogen [starch] synthase</fullName>
            <ecNumber evidence="8">2.4.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100183713</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">wild type</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Glycogen synthase participates in the glycogen biosynthetic process along with glycogenin and glycogen branching enzyme. Extends the primer composed of a few glucose units formed by glycogenin by adding new glucose units to it. In this context, glycogen synthase transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan.</text>
    </comment>
    <comment type="function">
        <text evidence="8">Transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>[(1-&gt;4)-alpha-D-glucosyl](n) + UDP-alpha-D-glucose = [(1-&gt;4)-alpha-D-glucosyl](n+1) + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18549"/>
            <dbReference type="Rhea" id="RHEA-COMP:9584"/>
            <dbReference type="Rhea" id="RHEA-COMP:9587"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15444"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.4.1.11"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:18550"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Glycan biosynthesis; glycogen biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the glycosyltransferase 3 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.11" evidence="8"/>
    <dbReference type="EMBL" id="EAAA01000719">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="EAAA01000720">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002125081.1">
        <property type="nucleotide sequence ID" value="XM_002125045.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F7APZ8"/>
    <dbReference type="FunCoup" id="F7APZ8">
        <property type="interactions" value="15"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000010169"/>
    <dbReference type="Ensembl" id="ENSCINT00000010169.3">
        <property type="protein sequence ID" value="ENSCINP00000010169.3"/>
        <property type="gene ID" value="ENSCING00000004933.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100183713"/>
    <dbReference type="KEGG" id="cin:100183713"/>
    <dbReference type="GeneTree" id="ENSGT00390000018612"/>
    <dbReference type="HOGENOM" id="CLU_015910_1_0_1"/>
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    <dbReference type="OMA" id="RDVRNHI"/>
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    <dbReference type="UniPathway" id="UPA00164"/>
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        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004373">
        <property type="term" value="F:alpha-1,4-glucan glucosyltransferase (UDP-glucose donor) activity"/>
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        <property type="project" value="GO_Central"/>
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        <property type="term" value="P:glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="Glycogen_synth"/>
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        <property type="entry name" value="GLYCOGEN [STARCH] SYNTHASE"/>
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        <property type="entry name" value="GLYCOGEN SYNTHASE"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0320">Glycogen biosynthesis</keyword>
    <keyword evidence="3 8" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="605"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="637"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="654"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023056"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00043883"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00047345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    </evidence>
    <sequence length="689" mass="78628" checksum="46BFD1E957F29901" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F7ARD3</accession>
    <name>F7ARD3_CIOIN</name>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
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    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01001018">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F7ARD3"/>
    <dbReference type="Ensembl" id="ENSCINT00000025966.2">
        <property type="protein sequence ID" value="ENSCINP00000025720.2"/>
        <property type="gene ID" value="ENSCING00000014150.2"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_843433_0_0_1"/>
    <dbReference type="InParanoid" id="F7ARD3"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5005677817" evidence="2">
        <location>
            <begin position="26"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="200"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000025720.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="330" mass="37714" checksum="0DC7CC6D297F1663" modified="2012-04-18" version="2">MQRPWVYEFALLCCIILLRVGLTKSVKLPCQQRYSEVATSGFATTFSTTTTQATTTTIHSQVTLAPSYVKANLCCGSRPFNSDVGVCCMGRVLRKSTRRCCLDGKHAPSESRCFCYPSSIWVTDEIDHLSGIEIQFTSLIEEMDATIEPLREQMVAAEEHIEFLDSKANSLYQEFLVHINMTRNRDPAEAEYITELISFLVANEANVENARKRLGALEKESMELLDAVVQSEEEEYSQTSSQTTRTTDVLTHSSLRRLRREIQTMNTTVETAEKLMDIVRQEIEQNLPVPEFNRQEAIRLTIERQNVLMNITRENRALSELRNRVQILTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>F7ASB7</accession>
    <name>F7ASB7_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FAD/NAD(P)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01000410">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F7ASB7"/>
    <dbReference type="STRING" id="7719.ENSCINP00000013726"/>
    <dbReference type="Ensembl" id="ENSCINT00000013726.3">
        <property type="protein sequence ID" value="ENSCINP00000013726.3"/>
        <property type="gene ID" value="ENSCING00000006694.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00640000091519"/>
    <dbReference type="HOGENOM" id="CLU_098147_0_0_1"/>
    <dbReference type="InParanoid" id="F7ASB7"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000521">
        <property type="entry name" value="FAD dependent oxidoreductase domain containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050982">
        <property type="entry name" value="Auxin_biosynth/cation_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43539:SF23">
        <property type="entry name" value="FAD-DEPENDENT OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43539">
        <property type="entry name" value="FLAVIN-BINDING MONOOXYGENASE-LIKE PROTEIN (AFU_ORTHOLOGUE AFUA_4G09220)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13738">
        <property type="entry name" value="Pyr_redox_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003347897" description="FAD/NAD(P)-binding domain-containing protein" evidence="2">
        <location>
            <begin position="20"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000013726.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="236" mass="26896" checksum="885E52ED93A5B3BD" modified="2012-04-18" version="2">MQLSVFVLVLFCLCLSVYGNNKHDGSIKHDDPMLNTETPNYKEYCVIGAGPGGLQIAYFLENAGRDYIVFEKGYGAGTFYLKYPIHRLLISINKRNTGKTNKEFNFRHDWNSLVSHDESLQMTKYSKDFFPPADVLVQYLNDYATKLNLKVQYNSTMHDILKRKDGLFQMWDQHNNAYTCKYVIMATGIATPNKPEFPGSELVDGYEDLPIDLDEYEGQSVLIIGRGNAGHETAQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>F7AWK4</accession>
    <name>F7AWK4_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Lipoxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">wild type</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the lipoxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002065">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F7AWK4"/>
    <dbReference type="STRING" id="7719.ENSCINP00000023023"/>
    <dbReference type="Ensembl" id="ENSCINT00000023269.2">
        <property type="protein sequence ID" value="ENSCINP00000023023.2"/>
        <property type="gene ID" value="ENSCING00000012298.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165874"/>
    <dbReference type="HOGENOM" id="CLU_004282_3_3_1"/>
    <dbReference type="InParanoid" id="F7AWK4"/>
    <dbReference type="OMA" id="WICDIFT"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016702">
        <property type="term" value="F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019369">
        <property type="term" value="P:arachidonate metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034440">
        <property type="term" value="P:lipid oxidation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019372">
        <property type="term" value="P:lipoxygenase pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.245.10">
        <property type="entry name" value="Lipoxygenase-1, Domain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.60.20">
        <property type="entry name" value="PLAT/LH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000907">
        <property type="entry name" value="LipOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013819">
        <property type="entry name" value="LipOase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036226">
        <property type="entry name" value="LipOase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020833">
        <property type="entry name" value="LipOase_Fe_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001024">
        <property type="entry name" value="PLAT/LH2_dom"/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Rash S."/>
                <person name="Saiga H."/>
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                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
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                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
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                <person name="Yagi K."/>
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                <person name="Wada S."/>
                <person name="Zhang C."/>
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                <person name="Detter C."/>
                <person name="Doggett N."/>
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                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
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            <dbReference type="PubMed" id="12481130"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">wild type</strain>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <ecNumber evidence="10">2.4.1.-</ecNumber>
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    </protein>
    <organism evidence="11 12">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">wild type</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Golgi apparatus membrane</location>
            <topology evidence="1 10">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the glycosyltransferase 31 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="10"/>
    <dbReference type="EMBL" id="EAAA01001108">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F7B4J3"/>
    <dbReference type="Ensembl" id="ENSCINT00000005021.3">
        <property type="protein sequence ID" value="ENSCINP00000005021.3"/>
        <property type="gene ID" value="ENSCING00000002476.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163442"/>
    <dbReference type="HOGENOM" id="CLU_036849_4_1_1"/>
    <dbReference type="InParanoid" id="F7B4J3"/>
    <dbReference type="OMA" id="WIVEENA"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016758">
        <property type="term" value="F:hexosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002659">
        <property type="entry name" value="Glyco_trans_31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214">
        <property type="entry name" value="BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214:SF283">
        <property type="entry name" value="N-ACETYLLACTOSAMINIDE BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE 4-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01762">
        <property type="entry name" value="Galactosyl_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8 10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000005021.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="283" mass="32551" checksum="A39E42DB1D4ECBD2" modified="2012-04-18" version="2">MDEGYKCGKVLRPKIDKETPWMTLLVKTAPKNFEYRNIIRKTWGGIQQFRGKIFKAIFMVGLAPSDDGVQTNLTVENGKYGDVLQCDFVDAYNALPTKVLSSLRWIVEENATSRFYSVTDDDCVINIPLLHDIFTKKDKGSVKDSLDDHNTVYCGYKYEAGAKPYRNNSSKWQMSMDLYSASNFPTYCMGAMWTLSFEIIRSLTCLSTVTDYADFYVEDVLITGILREKLGQGESNIKRIWKSHKVEKLRLLTLHTGKMEKKEGKKAMFEQWETWSKNLTTVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>F7B860</accession>
    <name>F7B860_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Mitochondrial potassium channel ATP-binding subunit</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">ATP-binding cassette sub-family B member 8, mitochondrial</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Mitochondrial sulfonylurea-receptor</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">wild type</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002917">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F7B860"/>
    <dbReference type="STRING" id="7719.ENSCINP00000003275"/>
    <dbReference type="Ensembl" id="ENSCINT00000003275.3">
        <property type="protein sequence ID" value="ENSCINP00000003275.3"/>
        <property type="gene ID" value="ENSCING00000001631.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159126"/>
    <dbReference type="HOGENOM" id="CLU_000604_1_9_1"/>
    <dbReference type="InParanoid" id="F7B860"/>
    <dbReference type="OMA" id="DPHANEC"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03249">
        <property type="entry name" value="ABC_MTABC3_MDL1_MDL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000403">
        <property type="entry name" value="ATP-binding cassette sub-family B member 8, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394">
        <property type="entry name" value="ATP-DEPENDENT PERMEASE MDL1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394:SF17">
        <property type="entry name" value="MITOCHONDRIAL POTASSIUM CHANNEL ATP-BINDING SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0630">Potassium</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003349152" description="Mitochondrial potassium channel ATP-binding subunit" evidence="15">
        <location>
            <begin position="19"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="16">
        <location>
            <begin position="64"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00040439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00041416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00042968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000003275.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="306" mass="33095" checksum="2537F8BA4DEF36E4" modified="2012-04-18" version="2">GQLMSFLVSAQMIQRSLASLSVLSGTAVRGVTAGGRVFEYIHKQPSIPTHGGRIIPYHCLLGHLKFDDVHFTYPQRQGHEVLKGVSLDIKPCQTVALCGLSGAGKSTVASLVERFYDPTSGKVTLDGINISELDPSWLRGKAIGYINQEPVLFSGSVEENIKYGNPEASKQEVIKAAKLANADAFIQNFPDGYNTIVGERGVSLSGGQKQRIAIARALLKNPSILILDEATSALDAESERLVQEALDHVMVNRTVLVIAHRLSTIKNADVIAVMKGGRVVEVGNHKDLKKKGGIYSELIKLQESNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>F7BA18</accession>
    <name>F7BA18_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">DNA replication licensing factor MCM5</fullName>
            <ecNumber evidence="14">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">wild type</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.</text>
    </comment>
    <comment type="function">
        <text evidence="14">Acts as component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="14">Component of the MCM2-7 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the MCM family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="14"/>
    <dbReference type="EMBL" id="EAAA01002665">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F7BA18"/>
    <dbReference type="FunCoup" id="F7BA18">
        <property type="interactions" value="633"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000006895"/>
    <dbReference type="Ensembl" id="ENSCINT00000006895.2">
        <property type="protein sequence ID" value="ENSCINP00000006895.2"/>
        <property type="gene ID" value="ENSCING00000003363.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286966"/>
    <dbReference type="HOGENOM" id="CLU_000995_7_2_1"/>
    <dbReference type="InParanoid" id="F7BA18"/>
    <dbReference type="OMA" id="ITYCKTR"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071162">
        <property type="term" value="C:CMG complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042555">
        <property type="term" value="C:MCM complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd17756">
        <property type="entry name" value="MCM5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.28.10:FF:000005">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1640.10:FF:000006">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000241">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1640.10">
        <property type="entry name" value="mini-chromosome maintenance (MCM) complex, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031327">
        <property type="entry name" value="MCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008048">
        <property type="entry name" value="MCM5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054125">
        <property type="entry name" value="MCM5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018525">
        <property type="entry name" value="MCM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041562">
        <property type="entry name" value="MCM_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027925">
        <property type="entry name" value="MCM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033762">
        <property type="entry name" value="MCM_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630:SF42">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21933">
        <property type="entry name" value="MCM5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17855">
        <property type="entry name" value="MCM_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14551">
        <property type="entry name" value="MCM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17207">
        <property type="entry name" value="MCM_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01657">
        <property type="entry name" value="MCMFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01661">
        <property type="entry name" value="MCMPROTEIN5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00350">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00847">
        <property type="entry name" value="MCM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50051">
        <property type="entry name" value="MCM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 14" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3 14" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 14" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MCM C-terminal AAA(+) ATPase" evidence="15">
        <location>
            <begin position="336"/>
            <end position="541"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00045440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU368063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000006895.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="740" mass="82660" checksum="1F48EFF62B1D9116" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>F7BFW8</accession>
    <accession>A0A1W2WMB0</accession>
    <name>F7BFW8_CIOIN</name>
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        <submittedName>
            <fullName evidence="2">Fatty acid-binding protein, liver-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC100178903</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.</text>
    </comment>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_026834934.1"/>
    </dbReference>
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        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000026694"/>
    <dbReference type="Ensembl" id="ENSCINT00000026940.2">
        <property type="protein sequence ID" value="ENSCINP00000026694.2"/>
        <property type="gene ID" value="ENSCING00000014865.2"/>
    </dbReference>
    <dbReference type="GeneID" id="100178903"/>
    <dbReference type="KEGG" id="cin:100178903"/>
    <dbReference type="GeneTree" id="ENSGT00940000157139"/>
    <dbReference type="HOGENOM" id="CLU_113772_4_0_1"/>
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    <dbReference type="OMA" id="GKFCHVQ"/>
    <dbReference type="OrthoDB" id="8501868at2759"/>
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        <property type="component" value="Chromosome 7"/>
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        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005504">
        <property type="term" value="F:fatty acid binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015908">
        <property type="term" value="P:fatty acid transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012674">
        <property type="entry name" value="Calycin"/>
    </dbReference>
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        <property type="entry name" value="Fatty_acid-bd"/>
    </dbReference>
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        <property type="entry name" value="ILBP"/>
    </dbReference>
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        <property type="entry name" value="FATTY ACID BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Lipocalins"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000026694.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="128" mass="14346" checksum="D1BE11B285499C9C" modified="2011-07-27" version="1">MVDAFLGTFVVETRNNFDGFMKGVGVPDEYIEIARKVFPTTEITKDGDSFTVKRIRPQKTTSNTFKLGEETEVETIKGDKVKVTINMVGSQMVTKGENYCVTTEMDGGKLKETITLKGHTLTRWSKRA</sequence>
</entry>
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        <submittedName>
            <fullName evidence="8">3'-5' exoribonuclease 1-like</fullName>
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        <name evidence="8" type="primary">LOC100182700</name>
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    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
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    <dbReference type="FunCoup" id="F7BKG7">
        <property type="interactions" value="140"/>
    </dbReference>
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    <dbReference type="Ensembl" id="ENSCINT00000012755.3">
        <property type="protein sequence ID" value="ENSCINP00000012755.3"/>
        <property type="gene ID" value="ENSCING00000006175.3"/>
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    <dbReference type="GeneTree" id="ENSGT00530000063205"/>
    <dbReference type="HOGENOM" id="CLU_037266_4_1_1"/>
    <dbReference type="InParanoid" id="F7BKG7"/>
    <dbReference type="OMA" id="MHSGQLM"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
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        <property type="project" value="GO_Central"/>
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        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
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    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="term" value="P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
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        <property type="project" value="GO_Central"/>
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        <property type="term" value="P:regulatory ncRNA-mediated gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="3'-5' exoribonuclease 1"/>
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        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="ERI-1_3'hExo-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013520">
        <property type="entry name" value="Ribonucl_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003034">
        <property type="entry name" value="SAP_dom"/>
    </dbReference>
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        <property type="entry name" value="SAP_dom_sf"/>
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        <property type="entry name" value="3'-5' EXONUCLEASE ERI1-RELATED"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR23044:SF61">
        <property type="entry name" value="3'-5' EXORIBONUCLEASE 1-RELATED"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
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                <person name="Miura S."/>
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                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <dbReference type="Ensembl" id="ENSCINT00000031605.1">
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
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</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
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    <protein>
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            <fullName evidence="1">Uncharacterized protein</fullName>
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    </protein>
    <organism evidence="1 2">
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        <name type="common">Transparent sea squirt</name>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
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                <person name="Hastings K.E."/>
                <person name="Ho I."/>
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                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
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                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
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                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
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                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
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                <person name="Morishita T."/>
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                <person name="Nakayama A."/>
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                <person name="Nomoto H."/>
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                <person name="Rigoutsos I."/>
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                <person name="Sasaki A."/>
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                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
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                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01001843">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002127330.1">
        <property type="nucleotide sequence ID" value="XM_002127294.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XM04"/>
    <dbReference type="Ensembl" id="ENSCINT00000031053.1">
        <property type="protein sequence ID" value="ENSCINP00000030686.1"/>
        <property type="gene ID" value="ENSCING00000020729.1"/>
    </dbReference>
    <dbReference type="GeneID" id="100179601"/>
    <dbReference type="KEGG" id="cin:100179601"/>
    <dbReference type="HOGENOM" id="CLU_1824631_0_0_1"/>
    <dbReference type="InParanoid" id="H2XM04"/>
    <dbReference type="OrthoDB" id="2109241at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039471">
        <property type="entry name" value="CXorf65-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33887">
        <property type="entry name" value="PB1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33887:SF5">
        <property type="entry name" value="PB1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15874">
        <property type="entry name" value="Il2rg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="82"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000030686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="141" mass="16214" checksum="8AF661B67F010EAE" modified="2012-04-18" version="1">MAFVTILYGVQIDLCDEVGLLKNLNSTSAKFANEILNKEREKLVLLQVEHGSTENISDGLHTNLVYKPLLVNDNIVTQRFKARLSRRDNSPQKSEEKYHSTRDKSKKNEVHPEDNHVDTTPMSGKYRNKKNSSRCRSRSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>H2XM66</accession>
    <name>H2XM66_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">E3 ubiquitin protein ligase</fullName>
            <ecNumber evidence="14">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">wild type</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 20">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 14">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the BRE1 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="14"/>
    <dbReference type="EMBL" id="EAAA01000699">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XM66"/>
    <dbReference type="FunCoup" id="H2XM66">
        <property type="interactions" value="1321"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000030748"/>
    <dbReference type="Ensembl" id="ENSCINT00000035084.1">
        <property type="protein sequence ID" value="ENSCINP00000030748.1"/>
        <property type="gene ID" value="ENSCING00000024382.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000002866"/>
    <dbReference type="InParanoid" id="H2XM66"/>
    <dbReference type="OMA" id="THIEIMT"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033503">
        <property type="term" value="C:HULC complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16704">
        <property type="entry name" value="RING-HC_RNF20-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000040">
        <property type="entry name" value="E3 ubiquitin protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058642">
        <property type="entry name" value="BRE1A/B-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013956">
        <property type="entry name" value="E3_ubiquit_lig_Bre1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23163:SF0">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE BRE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23163">
        <property type="entry name" value="RING FINGER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26052">
        <property type="entry name" value="BRE1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 14" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="11 14 15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 14" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9 14" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 13 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="17">
        <location>
            <begin position="932"/>
            <end position="971"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="209"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="331"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="433"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="572"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="677"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="812"/>
            <end position="909"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU365038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000030748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="985" mass="115196" checksum="0F8209D88C194E83" modified="2012-04-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>H2XMD6</accession>
    <name>H2XMD6_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Myosin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="H2XMD6"/>
    <dbReference type="STRING" id="7719.ENSCINP00000030819"/>
    <dbReference type="Ensembl" id="ENSCINT00000031410.1">
        <property type="protein sequence ID" value="ENSCINP00000030819.1"/>
        <property type="gene ID" value="ENSCING00000025130.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160705"/>
    <dbReference type="HOGENOM" id="CLU_000192_7_10_1"/>
    <dbReference type="InParanoid" id="H2XMD6"/>
    <dbReference type="OMA" id="RIMGSIM"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003774">
        <property type="term" value="F:cytoskeletal motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.720:FF:000001">
        <property type="entry name" value="Myosin heavy chain, muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140">
        <property type="entry name" value="MYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140:SF857">
        <property type="entry name" value="MYOSIN-11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0505">Motor protein</keyword>
    <keyword evidence="5 8" id="KW-0518">Myosin</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myosin motor" evidence="9">
        <location>
            <begin position="1"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000030819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="257" mass="29459" checksum="70003C979C04F93D" modified="2012-04-18" version="1">MSQGDVAVASIDDADELNATDTAFDVLGFSQDEKNGIYRIMGSIMHTGNMKFKQKPREEQAEADGTEDADKVTYLLGINSAEFVKSILSPRVRVGNDYVTKGQTVQQCYYSTGALSKAVYEKLFNWLVKRINETLSTRLPRSFFIGVLDIAGFEIFDFNSFEQLCINFTNEKLQQFFNHHMFVLEQEEYKREGIDWVFIDFGLDLQACIELIEKPLGIMSILEEECMFPKATDLTFKEKLYMNHLGKSNNFIKPRPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>H2XMX6</accession>
    <name>H2XMX6_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01000606">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XMX6"/>
    <dbReference type="Ensembl" id="ENSCINT00000034487.1">
        <property type="protein sequence ID" value="ENSCINP00000031009.1"/>
        <property type="gene ID" value="ENSCING00000020311.1"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1991833_0_0_1"/>
    <dbReference type="InParanoid" id="H2XMX6"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000031009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="125" mass="14047" checksum="AD0FD36BA8C8D044" modified="2012-04-18" version="1">MLEEVKDHHNIDNFDEFLKPSIPVERKKLKKNKVSPIPIPVEALLGSKPVSAAVDAFGELDDRASPEGISREAEHVEETNSGGDSEDDDDVASEESLAEVESQTIDEEEKVRIFQKINWQINHLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>H2XNA7</accession>
    <name>H2XNA7_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
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                <person name="Roe B.A."/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">wild type</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cytoplasm</location>
            <location evidence="1 10">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the GAMAD family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002910">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000031790"/>
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        <property type="protein sequence ID" value="ENSCINP00000031790.1"/>
        <property type="gene ID" value="ENSCING00000020850.1"/>
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        <property type="component" value="Chromosome 9"/>
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        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
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        <property type="term" value="C:cytoplasmic dynein complex"/>
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    </dbReference>
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        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
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        <property type="term" value="F:dynein intermediate chain binding"/>
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        <property type="term" value="P:microtubule-based movement"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Dynein light chain roadblock"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.30">
        <property type="entry name" value="Dynein light chain 2a, cytoplasmic"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="DYNLRB1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004942">
        <property type="entry name" value="Roadblock/LAMTOR2_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10779">
        <property type="entry name" value="DYNEIN LIGHT CHAIN ROADBLOCK"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Robl_LC7"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Robl_LC7"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Roadblock/LC7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="8 10" id="KW-0206">Cytoskeleton</keyword>
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    <keyword evidence="5 10" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7 10" id="KW-0505">Motor protein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Roadblock/LAMTOR2" evidence="11">
        <location>
            <begin position="4"/>
            <end position="92"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00025362"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRNR" id="PIRNR009998"/>
        </source>
    </evidence>
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            <dbReference type="SMART" id="SM00960"/>
        </source>
    </evidence>
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            <dbReference type="Ensembl" id="ENSCINP00000031790.1"/>
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    </evidence>
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    <sequence length="96" mass="10870" checksum="5108F045F5839D43" modified="2012-04-18" version="1">MSEVEETIKRIQSHKGVLGIIVVNSEGIPIRTSLDNSTTVQYAGLIHQLTMKARSTVRDIDPQNDLTFLRLRSKKHEIMVAPDKEYLLIVIQNPSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>H2XR12</accession>
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            <fullName evidence="7">GP-PDE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Tunicata</taxon>
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            <taxon>Phlebobranchia</taxon>
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            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">wild type</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycerophosphoryl diester phosphodiesterase family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001413">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="FunCoup" id="H2XR12">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000032096"/>
    <dbReference type="Ensembl" id="ENSCINT00000030418.1">
        <property type="protein sequence ID" value="ENSCINP00000032096.1"/>
        <property type="gene ID" value="ENSCING00000020212.1"/>
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    <dbReference type="GeneTree" id="ENSGT00440000033970"/>
    <dbReference type="HOGENOM" id="CLU_013007_2_0_1"/>
    <dbReference type="InParanoid" id="H2XR12"/>
    <dbReference type="OMA" id="LKVYHTA"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047389">
        <property type="term" value="F:glycerophosphocholine phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001070">
        <property type="term" value="F:starch binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046475">
        <property type="term" value="P:glycerophospholipid catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="GDPD_GDE5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.190:FF:000032">
        <property type="entry name" value="Glycerophosphoryl diester phosphodiesterase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR013784">
        <property type="entry name" value="Carb-bd-like_fold"/>
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    <dbReference type="InterPro" id="IPR002044">
        <property type="entry name" value="CBM20"/>
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        <property type="entry name" value="GDPD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030395">
        <property type="entry name" value="GP_PDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
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        <property type="entry name" value="GLYCEROPHOSPHOCHOLINE PHOSPHODIESTERASE GPCPD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22958">
        <property type="entry name" value="GLYCEROPHOSPHORYL DIESTER PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
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                <person name="Inaba K."/>
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            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">wild type</strain>
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    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    </evidence>
    <sequence length="244" mass="27721" checksum="357A2FE0149228C7" modified="2012-04-18" version="1">MMSQEVLDEISSELMRDQNYRSLTDQQKLNAKLVFQNALRLLSNGGILRLIILLSRIGRYGSESQVCASLYSAAQYENIEVETLRLDGVREFANTESSAKVMKLKVDLDKVTSNSVQAKYGLKLQLREIKEYMQLILRCVALWRWYSSSTTESEPNIARREATGSEMTSLPTTVKGIKFGKVIPPMVLVWIIVIVCFLTIIAFICFIIGTRVCFSGKSENRNGTSYKKKKKLGEWPRIVMSQEV</sequence>
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    <accession>H2XRX2</accession>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
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                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
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                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
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                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
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                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
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                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="6" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
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                <person name="Ueno K."/>
                <person name="Yamada L."/>
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                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
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                <person name="Lemaire P."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="6">wild type</strain>
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    <reference evidence="6" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <topology evidence="1">Multi-pass membrane protein</topology>
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        <property type="component" value="Chromosome 2"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="entry name" value="MFS_trans_sf"/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
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                <person name="Lee B.I."/>
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                <person name="Matassi G."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
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                <person name="Wiley G.B."/>
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                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
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                <person name="Lindquist E."/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Chapman J."/>
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                <person name="Di Gregorio A."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
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                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
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                <person name="Hastings K.E."/>
                <person name="Ho I."/>
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                <person name="Huang W."/>
                <person name="Kawashima T."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
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                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <source>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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            <authorList>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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        <source>
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    </evidence>
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        <source>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
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                <person name="Nomoto H."/>
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                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
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                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
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                <person name="Larimer F."/>
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                <person name="Doggett N."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">wild type</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
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                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
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                <person name="Doggett N."/>
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                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1">wild type</strain>
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    <reference evidence="1" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
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                <person name="Hastings K.E."/>
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                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
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        <property type="entry name" value="Znf_HIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048371">
        <property type="entry name" value="ZNHIT3_C"/>
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        <property type="entry name" value="BOX C_D SNORNA PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR13483:SF11">
        <property type="entry name" value="ZINC FINGER HIT DOMAIN-CONTAINING PROTEIN 3"/>
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        <property type="entry name" value="zf-HIT"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="HIT/MYND zinc finger-like"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
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    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 11" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="7"/>
            <end position="41"/>
        </location>
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        <source>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000035030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="129" mass="15176" checksum="795CC68EF99E8459" modified="2012-04-18" version="1">MTVKPTCQVCNENQHKYKCPQCYKRYCSVNCYKKHKSEDFCQANVEKPPKNGEKQLQCLEKQDEETEKISEEVLGRLKESEKLKDILKNPHLKKLLTEVDKNENTPQLINDLMQEPIFIEFADQCLDII</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>H2Y0F9</accession>
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        <recommendedName>
            <fullName evidence="3">Centromere protein X</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
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            <taxon>Phlebobranchia</taxon>
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        </lineage>
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    <reference evidence="10" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">wild type</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="8">Heterodimer with CENPX, sometimes called MHF; this interaction stabilizes both partners. MHF heterodimers can assemble to form tetrameric structures. MHF also coassemble with CENPT-CENPW heterodimers at centromeres to form the tetrameric CENP-T-W-S-X complex. Forms a discrete complex with FANCM and CENPX, called FANCM-MHF; this interaction, probably mediated by direct binding between CENPS and FANCM, leads to synergistic activation of double-stranded DNA binding and strongly stimulates FANCM-mediated DNA remodeling. Recruited by FANCM to the Fanconi anemia (FA) core complex, which consists of CENPS, CENPX, FANCA, FANCB, FANCC, FANCE, FANCF, FANCG, FANCL, FANCM, FAAP24 and FAAP100. The FA core complex associates with Bloom syndrome (BLM) complex, which consists of at least BLM, DNA topoisomerase 3-alpha (TOP3A), RMI1/BLAP75, RPA1/RPA70 and RPA2/RPA32. The super complex between FA and BLM is called BRAFT.</text>
    </comment>
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        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CENP-X/MHF2 family.</text>
    </comment>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="Ensembl" id="ENSCINT00000033267.1">
        <property type="protein sequence ID" value="ENSCINP00000035393.1"/>
        <property type="gene ID" value="ENSCING00000020146.1"/>
    </dbReference>
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    <dbReference type="InParanoid" id="H2Y0F9"/>
    <dbReference type="OMA" id="FKAKTIQ"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071821">
        <property type="term" value="C:FANCM-MHF complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="C:Fanconi anaemia nuclear complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CENTROMERE PROTEIN X"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CENTROMERE PROTEIN X"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00047146"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000035393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="82" mass="9030" checksum="75D96D3D00840511" modified="2012-04-18" version="1">MTSNKEGFSSEFAVKLLEKHFTDQNSTKISTKDGTGRKCAELLSVFVAEAASRAAKQAENEDAAICEIEHVEKILPQLLLDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>H2Y0J9</accession>
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        <recommendedName>
            <fullName evidence="2">TIR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
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        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
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        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
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            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </evidence>
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            <fullName evidence="3">DDRGK domain-containing protein 1</fullName>
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        <name evidence="11" type="primary">LOC100186953</name>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
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            <taxon>Eukaryota</taxon>
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        </lineage>
    </organism>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">wild type</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DDRGK1 family.</text>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="P:cartilage development"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="entry name" value="DDRGK domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="WH_DNA-bd_sf"/>
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        <property type="entry name" value="DDRGK DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Winged helix' DNA-binding domain"/>
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        </source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="294" mass="34095" checksum="F9FE88C59BDF2B44" modified="2012-04-18" version="1">KMNPVVYVIAATVVAIVLWFVTRGERNKPRATPGSNSPEGRAPVERAETVGGVRRRRRDVRRGQQEVERHEQEEQFDESGSEPEVALPAPSEGKIGAKKMKKLQEKEERRQMRQAMEMERLERRKEQAEKDEKLRKLEELKKAEEEKKAEEERKIQEEKERKEHEEYLKLKEMFSVEDEGQADVTSEEQSQNMLQEFVQYIKDTKVVLLEDLASHFGLRTQQAIDRVQDMLKDEIITGVIDDRGKFIYISMEELKSVAKFVQQRGRISISELAQASNTLVNLKPEKTLTLSQAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
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    <name>H2Y0M3_CIOIN</name>
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            <fullName evidence="3">CHRD domain-containing protein</fullName>
        </recommendedName>
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        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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    <reference evidence="5" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="H2Y0M3"/>
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        <property type="gene ID" value="ENSCING00000023715.1"/>
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    <dbReference type="HOGENOM" id="CLU_1109121_0_0_1"/>
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    <dbReference type="OMA" id="YTHVTEM"/>
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        <property type="component" value="Chromosome 6"/>
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    <dbReference type="InterPro" id="IPR052278">
        <property type="entry name" value="Chordin-like_regulators"/>
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        <property type="entry name" value="CHRD"/>
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    <dbReference type="PANTHER" id="PTHR46526">
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        <location>
            <begin position="7"/>
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        <location>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000035457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
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        </source>
    </evidence>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">wild type</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01000727">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2Y1Q6"/>
    <dbReference type="Ensembl" id="ENSCINT00000036730.1">
        <property type="protein sequence ID" value="ENSCINP00000035840.1"/>
        <property type="gene ID" value="ENSCING00000024739.1"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2176646_0_0_1"/>
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    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 11"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000035840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="110" mass="12669" checksum="365A42FC3DA025AC" modified="2012-04-18" version="1">AHTLGKSDDSRRCRRNLVLLENWFPQAIYNLLYEESRTLVPTKITLVGCDIFRFTLLRSVDLLHYVDTETGTKPFADAEPPNTVCCVSYRRLSKPEHKYPCDLFGCLCVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>H2Y2X3</accession>
    <accession>A0A1W2WDP2</accession>
    <name>H2Y2X3_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mammalian ependymin-related protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100187093</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
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                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
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                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
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                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="5">wild type</strain>
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    <reference evidence="5" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <text evidence="1">Binds anionic lipids and gangliosides at acidic pH.</text>
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    <comment type="similarity">
        <text evidence="2">Belongs to the ependymin family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="ENSCINP00000036258.1"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0007160">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR10697:SF1">
        <property type="entry name" value="MAMMALIAN EPENDYMIN-RELATED PROTEIN 1"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00811">
        <property type="entry name" value="Ependymin"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014093647" description="Mammalian ependymin-related protein 1" evidence="4">
        <location>
            <begin position="16"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000036258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="200" mass="23492" checksum="217FCE54B3FB514F" modified="2012-04-18" version="1">MKFFLLVALFGLTYAQDPRCAAPGYFQARVRSYDHETNYFRDGRFSYDAHEQRVRMVEVLQENATRPQRDELILHREKKRYVVDLHTRKCNVSTVDFHFHPISVDEPARFLGYSIVGTLDEPEMGMMVGSWHHRSEGNDIRGNLTQSFTRDECIPVSEVFYGETVKDHQMLHFHRTYFDVTLGLDSANVFDIPKECIHRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>H2Y377</accession>
    <name>H2Y377_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="H2Y377"/>
    <dbReference type="STRING" id="7719.ENSCINP00000036362"/>
    <dbReference type="Ensembl" id="ENSCINT00000034142.1">
        <property type="protein sequence ID" value="ENSCINP00000036362.1"/>
        <property type="gene ID" value="ENSCING00000024599.1"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1772249_0_0_1"/>
    <dbReference type="InParanoid" id="H2Y377"/>
    <dbReference type="OMA" id="DCEIYRI"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002013">
        <property type="entry name" value="SAC_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02383">
        <property type="entry name" value="Syja_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAC" evidence="1">
        <location>
            <begin position="59"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000036362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="147" mass="16294" checksum="0F54F5B7A24D5680" modified="2012-04-18" version="1">MSTSRQLKVYQDKSRPDSFLLEHRTQEHLLLLQDNCACALSTSDGNELKSSCTKIADTYGCLGVIILNKDAEALVLITGCRVVGKLLDCEIYRISDVSFISLKDASDVTSSLSDLKRLLCSGSFYFSTCGNDDQKNLNLTRTLQKQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-16" modified="2026-01-28" version="118" xmlns="https://uniprot.org/uniprot">
    <accession>Q1RL32</accession>
    <accession>A0A1W2VNR3</accession>
    <accession>F7AYL3</accession>
    <name>Q1RL32_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="7 8">Zinc finger protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">Ci-ZF(FYVE)-9</name>
        <name evidence="8" type="synonym">zf(fyve)-9</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2006" name="Dev. Biol." volume="292" first="546" last="554">
            <title>Systematic analysis of embryonic expression profiles of zinc finger genes in Ciona intestinalis.</title>
            <authorList>
                <person name="Miwata K."/>
                <person name="Chiba T."/>
                <person name="Horii R."/>
                <person name="Yamada L."/>
                <person name="Kubo A."/>
                <person name="Miyamura D."/>
                <person name="Satoh N."/>
                <person name="Satou Y."/>
            </authorList>
            <dbReference type="PubMed" id="16519883"/>
            <dbReference type="DOI" id="10.1016/j.ydbio.2006.01.024"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="BR000202">
        <property type="protein sequence ID" value="FAA00233.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001041457.1">
        <property type="nucleotide sequence ID" value="NM_001047992.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1RL32"/>
    <dbReference type="STRING" id="7719.ENSCINP00000007285"/>
    <dbReference type="Ensembl" id="ENSCINT00000007285.3">
        <property type="protein sequence ID" value="ENSCINP00000007285.3"/>
        <property type="gene ID" value="ENSCING00000003542.3"/>
    </dbReference>
    <dbReference type="GeneID" id="751564"/>
    <dbReference type="KEGG" id="cin:751564"/>
    <dbReference type="CTD" id="751564"/>
    <dbReference type="GeneTree" id="ENSGT00940000156408"/>
    <dbReference type="HOGENOM" id="CLU_064864_1_0_1"/>
    <dbReference type="OMA" id="PVRVCEH"/>
    <dbReference type="OrthoDB" id="70570at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007032">
        <property type="term" value="P:endosome organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008333">
        <property type="term" value="P:endosome to lysosome transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd15717">
        <property type="entry name" value="FYVE_PKHF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01218">
        <property type="entry name" value="PH_Phafin2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000167">
        <property type="entry name" value="Pleckstrin homology domain-containing family F member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051765">
        <property type="entry name" value="PH_domain-containing_F"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037871">
        <property type="entry name" value="PH_Phafin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055251">
        <property type="entry name" value="SOS1_NGEF_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46280:SF3">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY DOMAIN-CONTAINING FAMILY F MEMBER 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46280">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY DOMAIN-CONTAINING FAMILY F MEMBER 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22697">
        <property type="entry name" value="SOS1_NGEF_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PH" evidence="5">
        <location>
            <begin position="35"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="FYVE-type" evidence="6">
        <location>
            <begin position="163"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="FAA00233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000007285.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="258" mass="28854" checksum="D5B044080004D60C" modified="2006-05-16" version="1">MVERLANTEINARRISHVESCFGSSGKPLFHPSRVLVGEGVLTKLCRKKPKARQFFLFNDVLVYGNIVINKKRYNKQSVLSLRNVRVEEIPDNEETAAADGSDTLKNGWLIISPSKSFAVYAQTAMEKEEWLTHMTRCIDEEKKKSGPSISVTDQFAPTWVPDSEAVVCMRCKKTRFTTLQRKHHCRKCGLVVCNACSTKKCIIQHQSAKPLRVCDVCYQSLQNGSNTPEVTASNVPNNKLAFGDTSYSSDSESSNEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-06-07" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1W2WEA2</accession>
    <accession>F6XGG4</accession>
    <name>A0A1W2WEA2_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Adenylate kinase</fullName>
            <ecNumber evidence="7">2.7.4.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">ATP-AMP transphosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">ATP:AMP phosphotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Adenylate kinase cytosolic and mitochondrial</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Adenylate monophosphate kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100181027</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">wild type</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism. Adenylate kinase activity is critical for regulation of the phosphate utilization and the AMP de novo biosynthesis pathways.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>AMP + ATP = 2 ADP</text>
            <dbReference type="Rhea" id="RHEA:12973"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>GTP + AMP = GDP + ADP</text>
            <dbReference type="Rhea" id="RHEA:29863"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="7">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
            <location evidence="7">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Mitochondrion intermembrane space</location>
        </subcellularLocation>
        <text evidence="7">Predominantly mitochondrial.</text>
    </comment>
    <comment type="domain">
        <text evidence="7">Consists of three domains, a large central CORE domain and two small peripheral domains, NMPbind and LID, which undergo movements during catalysis. The LID domain closes over the site of phosphoryl transfer upon ATP binding. Assembling and dissambling the active center during each catalytic cycle provides an effective means to prevent ATP hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the adenylate kinase family. AK2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.3" evidence="7"/>
    <dbReference type="EMBL" id="EAAA01001279">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002129443.1">
        <property type="nucleotide sequence ID" value="XM_002129407.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009860848.1">
        <property type="nucleotide sequence ID" value="XM_009862546.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1W2WEA2"/>
    <dbReference type="FunCoup" id="A0A1W2WEA2">
        <property type="interactions" value="680"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000007995"/>
    <dbReference type="Ensembl" id="ENSCINT00000007995.3">
        <property type="protein sequence ID" value="ENSCINP00000007995.3"/>
        <property type="gene ID" value="ENSCING00000003848.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100181027"/>
    <dbReference type="KEGG" id="cin:100181027"/>
    <dbReference type="GeneTree" id="ENSGT00940000154576"/>
    <dbReference type="InParanoid" id="A0A1W2WEA2"/>
    <dbReference type="OMA" id="VYHEQTA"/>
    <dbReference type="OrthoDB" id="439792at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004017">
        <property type="term" value="F:AMP kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006172">
        <property type="term" value="P:ADP biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046033">
        <property type="term" value="P:AMP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046034">
        <property type="term" value="P:ATP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060218">
        <property type="term" value="P:hematopoietic stem cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd01428">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000106">
        <property type="entry name" value="Adenylate kinase mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00235">
        <property type="entry name" value="Adenylate_kinase_Adk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03168">
        <property type="entry name" value="Adenylate_kinase_AK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006259">
        <property type="entry name" value="Adenyl_kin_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000850">
        <property type="entry name" value="Adenylat/UMP-CMP_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033690">
        <property type="entry name" value="Adenylat_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007862">
        <property type="entry name" value="Adenylate_kinase_lid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028587">
        <property type="entry name" value="AK2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01351">
        <property type="entry name" value="adk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001381">
        <property type="entry name" value="PRK00279.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011100">
        <property type="entry name" value="PRK14527.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23359">
        <property type="entry name" value="NUCLEOTIDE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00406">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05191">
        <property type="entry name" value="ADK_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00094">
        <property type="entry name" value="ADENYLTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00113">
        <property type="entry name" value="ADENYLATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 7 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="2 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Adenylate kinase active site lid" evidence="9">
        <location>
            <begin position="141"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="NMPbind" evidence="7">
        <location>
            <begin position="44"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="LID" evidence="7">
        <location>
            <begin position="140"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="24"/>
            <end position="29"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="71"/>
            <end position="73"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="99"/>
            <end position="102"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="150"/>
            <end position="151"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF05191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000007995.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="230" mass="25234" checksum="24E7321D0533797A" modified="2017-06-07" version="1">MAPKDAVGVDNRKGVRAILLGPPGAGKGTQAPKLAEEFCACHLSTGDMLRALVAGGSKLGQKVKDIMNRGALVSDDIVVEMINQNLDKPECSKGFLLDGFPRTVVQAEKLDELLEKRNSELDSVIEFGIDDSLLVSRICGRLLHKPSGRTYHTEFNPPKKSMTDDITGEPLIRRADDNESALKTRLAGYHKMTVPLVDYYAKKQLHTRVDASQSPDVVFKSIRATFLKCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-06-07" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1W3JDM4</accession>
    <accession>F6VZ08</accession>
    <name>A0A1W3JDM4_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Leucine-rich repeat-containing protein 27</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100177846</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">wild type</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01002709">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002130075.1">
        <property type="nucleotide sequence ID" value="XM_002130039.4"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009859355.1">
        <property type="nucleotide sequence ID" value="XM_009861053.3"/>
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    <dbReference type="AlphaFoldDB" id="A0A1W3JDM4"/>
    <dbReference type="STRING" id="7719.ENSCINP00000015127"/>
    <dbReference type="Ensembl" id="ENSCINT00000015127.3">
        <property type="protein sequence ID" value="ENSCINP00000015127.3"/>
        <property type="gene ID" value="ENSCING00000007369.3"/>
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    <dbReference type="GeneID" id="100177846"/>
    <dbReference type="KEGG" id="cin:100177846"/>
    <dbReference type="eggNOG" id="KOG0620">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000170679"/>
    <dbReference type="InParanoid" id="A0A1W3JDM4"/>
    <dbReference type="OMA" id="SHMIVEI"/>
    <dbReference type="OrthoDB" id="2021138at2759"/>
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        <property type="component" value="Chromosome 8"/>
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        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Leu-rich_rpt"/>
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        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050333">
        <property type="entry name" value="SLRP"/>
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    <dbReference type="PANTHER" id="PTHR45712">
        <property type="entry name" value="AGAP008170-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45712:SF22">
        <property type="entry name" value="INSULIN-LIKE GROWTH FACTOR-BINDING PROTEIN COMPLEX ACID LABILE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="285"/>
            <end position="327"/>
        </location>
    </feature>
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        <location>
            <begin position="380"/>
            <end position="460"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="285"/>
            <end position="298"/>
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        <location>
            <begin position="306"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="446"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000015127.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="486" mass="56111" checksum="BEEA1D1116C72BF7" modified="2017-06-07" version="1">MHSFDNSEPGRTTVNKKLGNKDNEVVKLIKNQSHDDAFLDLCNRGIQILPDQLYTLPHIQHLYLEGNQISQLPNNFFDRLPTLQWLDLRNNQLKAIPVSIGRHQSIKKLLLEGNCLTHLPLQMGLTKTLTGLNLRGNPLVVPPQHITDQGVYVILSYLWQELQNRTKTPYDEVSPAVEKLSIIDDESNNSEEDEDMLTDAAIFTGRNIPSPLSDDFELVRHGPTPMSASLHKPVSYQEYKGRFQGNSLLEGLRNKSRNSPKEISLVDIHHKDDVRKDQLRQMREMKQMNEDQRRKDKTVLSNWRHETRRRQRQSRSKTADVSIRPPPFASDEQLVIHSDVREEKVETVLPGEHKLRVKHTVPIKRSHMIVEIPNAMNEVNQTESVDEEKKEDKNEPFVGKMSRPNSVARSRTNDEIMQRMKQHSSQLHKQFKKQRKEVSSTAPSKELQRELLDRKMSKDPQLEYRFRAFTGDTASAHSSSNKTTSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-06-07" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1W3JUM5</accession>
    <accession>H2XQQ0</accession>
    <name>A0A1W3JUM5_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 11">Zinc finger Ran-binding domain-containing protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">zf(rbz)</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10 11">Belongs to the ZRANB2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_026696139.1">
        <property type="nucleotide sequence ID" value="XM_026840338.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1W3JUM5"/>
    <dbReference type="STRING" id="7719.ENSCINP00000031984"/>
    <dbReference type="Ensembl" id="ENSCINT00000031885.1">
        <property type="protein sequence ID" value="ENSCINP00000031984.1"/>
        <property type="gene ID" value="ENSCING00000020967.1"/>
    </dbReference>
    <dbReference type="GeneID" id="778830"/>
    <dbReference type="CTD" id="778830"/>
    <dbReference type="GeneTree" id="ENSGT00730000111078"/>
    <dbReference type="InParanoid" id="A0A1W3JUM5"/>
    <dbReference type="OMA" id="CNICHAP"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001530">
        <property type="term" value="F:lipopolysaccharide binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1060.10:FF:000004">
        <property type="entry name" value="Zinc finger Ran-binding domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1060.10">
        <property type="entry name" value="Zinc finger, RanBP2-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001876">
        <property type="entry name" value="Znf_RanBP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036443">
        <property type="entry name" value="Znf_RanBP2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017337">
        <property type="entry name" value="ZRANB2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12999:SF17">
        <property type="entry name" value="ZINC FINGER RAN-BINDING DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12999">
        <property type="entry name" value="ZINC FINGER RAN-BINDING DOMAIN-CONTAINING PROTEIN 2 ZRANB2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00641">
        <property type="entry name" value="Zn_ribbon_RanBP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037956">
        <property type="entry name" value="UCP037956_ZnF_Ran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00547">
        <property type="entry name" value="ZnF_RBZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90209">
        <property type="entry name" value="Ran binding protein zinc finger-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01358">
        <property type="entry name" value="ZF_RANBP2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50199">
        <property type="entry name" value="ZF_RANBP2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RanBP2-type" evidence="14">
        <location>
            <begin position="9"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="domain" description="RanBP2-type" evidence="14">
        <location>
            <begin position="63"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="139"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="139"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="150"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="170"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="218"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR037956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000031984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="237" mass="27084" checksum="100E29AB8617CEED" modified="2017-06-07" version="1">MSRSGFQFSEGDWTCPGCGNVNFARRMECNRCKEARNIGITKVKKGGVQIGKQAAEKSKGLFSADDWMCKTCGNVNWARRNDCNMCNTPKVGVQEERTGLGGGFNERQGVEYKKREDSDDEYDIYGRLKKNRGKKSIENQLTDRKIENREDSEEDESDDSDADLSKYKLEEDDDDDDDGNVDTSKYDLDVSDQEEDQSSKKYLHPSNEKSLSSKKRQIKESNRSRSRSPRDRKLSSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-06-07" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1W5B320</accession>
    <accession>H2XWU8</accession>
    <name>A0A1W5B320_CIOIN</name>
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        <submittedName>
            <fullName evidence="2">Rhodanese domain-containing protein CG4456-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC100180651</name>
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    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01000034">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="RefSeq" id="XP_018666900.1">
        <property type="nucleotide sequence ID" value="XM_018811355.2"/>
    </dbReference>
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    <dbReference type="FunCoup" id="A0A1W5B320">
        <property type="interactions" value="4"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000034132"/>
    <dbReference type="Ensembl" id="ENSCINT00000030616.1">
        <property type="protein sequence ID" value="ENSCINP00000034132.1"/>
        <property type="gene ID" value="ENSCING00000018185.1"/>
    </dbReference>
    <dbReference type="GeneID" id="100180651"/>
    <dbReference type="KEGG" id="cin:100180651"/>
    <dbReference type="eggNOG" id="KOG1530">
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    <dbReference type="GeneTree" id="ENSGT00940000163155"/>
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        <property type="component" value="Chromosome 1"/>
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        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44086">
        <property type="entry name" value="THIOSULFATE SULFURTRANSFERASE RDL2, MITOCHONDRIAL-RELATED"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR44086:SF10">
        <property type="entry name" value="THIOSULFATE SULFURTRANSFERASE_RHODANESE-LIKE DOMAIN-CONTAINING PROTEIN 3"/>
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    </dbReference>
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        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
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    </dbReference>
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        <property type="entry name" value="RHODANESE_3"/>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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            <end position="125"/>
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    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000034132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="126" mass="13502" checksum="C162FD6FA7C8CA20" modified="2017-06-07" version="1">MAHQGAPLPVLDDSQKISVGELKDLLAKSDPLVVDVRNPSEIEASGSIGSKKNINIPITDLDKELSLSNDEFKAKYGLDKPQGDGSDVIFHCMKGGRGSRAVVVAEKHGMKKARNLEGGYGLWSSQ</sequence>
</entry>
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    <accession>F6PI29</accession>
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            <fullName evidence="7">Zonadhesin</fullName>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
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                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
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        <property type="nucleotide sequence ID" value="XM_002125161.5"/>
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    <dbReference type="Ensembl" id="ENSCINT00000020271.3">
        <property type="protein sequence ID" value="ENSCINP00000020271.3"/>
        <property type="gene ID" value="ENSCING00000010151.3"/>
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    <dbReference type="KEGG" id="cin:100177372"/>
    <dbReference type="GeneTree" id="ENSGT00940000167195"/>
    <dbReference type="HOGENOM" id="CLU_325691_0_0_1"/>
    <dbReference type="InParanoid" id="F6PI29"/>
    <dbReference type="OMA" id="ETHLCAE"/>
    <dbReference type="OrthoDB" id="6236007at2759"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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    <dbReference type="GO" id="GO:0030414">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Ser_inhib-like_sf"/>
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        <property type="entry name" value="Thyroglobulin_1"/>
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        <property type="entry name" value="ACCESSORY GLAND PROTEIN ACP62F-RELATED"/>
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        <property type="entry name" value="Thyroglobulin type-1 domain"/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
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                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
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                <person name="Putnam N."/>
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                <person name="Awazu S."/>
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                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
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                <person name="Morishita T."/>
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                <person name="Zhang C."/>
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                <person name="Doggett N."/>
                <person name="Glavina T."/>
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                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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            <authorList>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
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                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
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                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">wild type</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01000081">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6PLI6"/>
    <dbReference type="STRING" id="7719.ENSCINP00000020204"/>
    <dbReference type="Ensembl" id="ENSCINT00000020204.3">
        <property type="protein sequence ID" value="ENSCINP00000020204.3"/>
        <property type="gene ID" value="ENSCING00000020468.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163713"/>
    <dbReference type="HOGENOM" id="CLU_061199_2_2_1"/>
    <dbReference type="InParanoid" id="F6PLI6"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040632">
        <property type="entry name" value="Sulfotransfer_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36978:SF4">
        <property type="entry name" value="P-LOOP CONTAINING NUCLEOSIDE TRIPHOSPHATE HYDROLASE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36978">
        <property type="entry name" value="P-LOOP CONTAINING NUCLEOTIDE TRIPHOSPHATE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17784">
        <property type="entry name" value="Sulfotransfer_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000020204.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="193" mass="22854" checksum="A2029B5851FB2A8D" modified="2012-04-18" version="2">MLPTCVIMKAIYAGFPKTGTKTIEAVFREFGYNTYDFLENSYYLGKDWRRIIKDGGTVEDFRRMYKDVDAVVDGPCFMFWEEILEAFPEAKIIFSVRDEESWLPSFKKQIRSLATNHMYVLMQVLSYSGWRHFQFSNACALVQLGSTINWPWCEYPYSPTVWRRIYRNHNNYVLHVSTYGCNNIYSNILQDVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>F6PML6</accession>
    <name>F6PML6_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">wild type</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="EAAA01000148">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6PML6"/>
    <dbReference type="FunCoup" id="F6PML6">
        <property type="interactions" value="10"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000025078"/>
    <dbReference type="Ensembl" id="ENSCINT00000025324.2">
        <property type="protein sequence ID" value="ENSCINP00000025078.2"/>
        <property type="gene ID" value="ENSCING00000013721.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162775"/>
    <dbReference type="HOGENOM" id="CLU_040940_4_1_1"/>
    <dbReference type="InParanoid" id="F6PML6"/>
    <dbReference type="OMA" id="WERPWDR"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010945">
        <property type="term" value="F:coenzyme A diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03426">
        <property type="entry name" value="NUDIX_CoAse_Nudt7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045121">
        <property type="entry name" value="CoAse"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12992:SF11">
        <property type="entry name" value="MITOCHONDRIAL COENZYME A DIPHOSPHATASE NUDT8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12992">
        <property type="entry name" value="NUDIX HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0464">Manganese</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="7">
        <location>
            <begin position="65"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000025078.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="230" mass="25871" checksum="93A496B22E4C8B74" modified="2012-04-18" version="2">MLRTSICIRLFKSRIKYNTRTAQNQLHATHCIFSTENVKKHVVKIAESLTSELSYSNSIKRLKLKNQAAVLVPLCTVNGELCLLFTVRSNNLPTHKGQISFPGGGVKHGDTGAVDAALRETEEEIGFPRNKVDVWTTLPHISNHTRTAAVIPVVGYLGEVNIEDMVPDRNEVTEIFTVSLTNLVNNASYTRFRIKAKKRSEHDYVMPVYFTQYRIWGLTANIVDTVLSIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>F6PN33</accession>
    <name>F6PN33_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Tyrosine-protein kinase</fullName>
            <ecNumber evidence="10">2.7.10.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 10">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the protein kinase superfamily. Tyr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.2" evidence="10"/>
    <dbReference type="AlphaFoldDB" id="F6PN33"/>
    <dbReference type="FunCoup" id="F6PN33">
        <property type="interactions" value="7"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000004763"/>
    <dbReference type="Ensembl" id="ENSCINT00000004763.3">
        <property type="protein sequence ID" value="ENSCINP00000004763.3"/>
        <property type="gene ID" value="ENSCING00000016914.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000170881"/>
    <dbReference type="HOGENOM" id="CLU_000288_7_2_1"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="1 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 10" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="SH2" evidence="11">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="125"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
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        <source>
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        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00051245"/>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50001"/>
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        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000004763.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="390" mass="44603" checksum="3C74FABDDD96CCA6" modified="2012-04-18" version="2">WFQRDMTRQRAEYLLKQDGKEGNFIVRNSSQPSVVYTLSIVTKGGQINGPAIVKHYHIRKNQNDEFYLSEKHFLPTIKELINYHKHNAGGLVTRLRQAPVSIAGNSQPATHLFSNSHWEIDINELQFMKELGSGQFGVVQLASIRGKQLVAVKIMKEGTMEEDHFIEEAEVMTKLTHPNLVELYGVVVKQRPICIVTEFMEEGCLLDYIRKHTHLQNHPETLLDMSCQICSAMKYLETNNLIHRDLAARNCLVGKNMLVKVADFGLTRYVLDDEYTSSLGSKFPIKWAAPEVLNFTKFSSKSDVWAFGILMWELFTGGKMPYRALSNTEVVERVAVGHRLERPQSCPHDVYLLMLKTWDMEPEKRPSFEILSMEIEDIVDIVNAACMPEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>F6Q2S6</accession>
    <accession>A0A1W2W586</accession>
    <name>F6Q2S6_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized LOC100177538</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100177538</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_002121597.1">
        <property type="nucleotide sequence ID" value="XM_002121561.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6Q2S6"/>
    <dbReference type="Ensembl" id="ENSCINT00000001340.3">
        <property type="protein sequence ID" value="ENSCINP00000001340.3"/>
        <property type="gene ID" value="ENSCING00000000731.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100177538"/>
    <dbReference type="KEGG" id="cin:100177538"/>
    <dbReference type="GeneTree" id="ENSGT00520000058756"/>
    <dbReference type="HOGENOM" id="CLU_101213_2_0_1"/>
    <dbReference type="InParanoid" id="F6Q2S6"/>
    <dbReference type="OMA" id="VPPKKYM"/>
    <dbReference type="OrthoDB" id="336321at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="3">
        <location>
            <begin position="70"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="1">
        <location>
            <begin position="70"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="100"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000001340.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="167" mass="19473" checksum="1333D129FE06F237" modified="2011-07-27" version="1">MNLLADYDSDSEDEKPKAKVILPSADKVLKSATQSSLFTTRFDKAEQEDREVLEHHVTMVESKDKVTNVGGRKVCWMYRKGRCRFGKKCQMFHDSELREQRTIKRPSNQDQPISFKDDGFQIQTDQSEQPKKKRYGVTNTLAPPNKALAQIKRIKKQEQYFDRTKKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>F6Q8X5</accession>
    <name>F6Q8X5_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01000088">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6Q8X5"/>
    <dbReference type="FunCoup" id="F6Q8X5">
        <property type="interactions" value="97"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000018499"/>
    <dbReference type="Ensembl" id="ENSCINT00000018499.3">
        <property type="protein sequence ID" value="ENSCINP00000018499.3"/>
        <property type="gene ID" value="ENSCING00000009114.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000168405"/>
    <dbReference type="HOGENOM" id="CLU_048526_0_0_1"/>
    <dbReference type="InParanoid" id="F6Q8X5"/>
    <dbReference type="OMA" id="CSITIRF"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061817">
        <property type="term" value="P:endoplasmic reticulum-plasma membrane tethering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04050">
        <property type="entry name" value="C2B_Synaptotagmin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04030">
        <property type="entry name" value="C2C_KIAA1228"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000395">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037752">
        <property type="entry name" value="C2C_KIAA1228"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037749">
        <property type="entry name" value="Ext_Synaptotagmin_C2B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051634">
        <property type="entry name" value="Extended_Synaptotagmin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45761">
        <property type="entry name" value="EXTENDED SYNAPTOTAGMIN-LIKE PROTEIN 2, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45761:SF1">
        <property type="entry name" value="EXTENDED SYNAPTOTAGMIN-LIKE PROTEIN 2, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="2">
        <location>
            <begin position="63"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="2">
        <location>
            <begin position="323"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="208"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="225"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="238"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="271"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000018499.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="449" mass="50466" checksum="5DABE8F3A45D692C" modified="2012-04-18" version="2">FDDDGALNKSDNLGMCSIPVKSVFKQGIIDEWVQLSDVSTGAIHVRLEFYELSDNPKDLKGTLTQKALNHSHMNDKLFSSFLNIYVDGAQNLPEFNQECYDANPQLKITLPGKEPLKTRVAMHTNNPVWEENFHVLISHPELDLVTFQMENDHGNQNLGFMKFPLKHLLRAQDMTIEHPFTLKSSGPSSVLNMRLTLRILKLKSLSESPEAKFTVHTKPKEPMDNTENTSNSPSVDASEPSESGSSFSRSTGPTEIPGLETKRMSPLTEGITPSPSGLRSNTSLDRTSSAASEISNVGEISNLRKRLNARVQNSNNIETNDNGLGRLELTIRYYNKHLVVVVLRAANLIVCDDDEKTSDPYVRVYILPDKRSRKKTKVIKNNLNPVWDQRLEFDVSKSEVMHKKLHVSVKNQTGFLSSEKVLMGQVIVDLSKLDLHQPTTEWYNLQVAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>F6Q9P9</accession>
    <name>F6Q9P9_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glycine--tRNA ligase</fullName>
            <ecNumber evidence="4">6.1.1.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Diadenosine tetraphosphate synthetase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">wild type</strain>
        </source>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>2 ATP + H(+) = P(1),P(4)-bis(5'-adenosyl) tetraphosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:34935"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58141"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:34936"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>tRNA(Gly) + glycine + ATP = glycyl-tRNA(Gly) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:16013"/>
            <dbReference type="Rhea" id="RHEA-COMP:9664"/>
            <dbReference type="Rhea" id="RHEA-COMP:9683"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78522"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.14"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:16014"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.14" evidence="4"/>
    <dbReference type="EMBL" id="EAAA01001792">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6Q9P9"/>
    <dbReference type="FunCoup" id="F6Q9P9">
        <property type="interactions" value="701"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000018157"/>
    <dbReference type="Ensembl" id="ENSCINT00000018157.4">
        <property type="protein sequence ID" value="ENSCINP00000018157.4"/>
        <property type="gene ID" value="ENSCING00000008937.4"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153759"/>
    <dbReference type="HOGENOM" id="CLU_015515_1_0_1"/>
    <dbReference type="InParanoid" id="F6Q9P9"/>
    <dbReference type="OMA" id="MEMQYFV"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004820">
        <property type="term" value="F:glycine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070150">
        <property type="term" value="P:mitochondrial glycyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00774">
        <property type="entry name" value="GlyRS-like_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00858">
        <property type="entry name" value="GlyRS_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.230:FF:000001">
        <property type="entry name" value="Glycine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.720.200:FF:000001">
        <property type="entry name" value="Glycine--tRNA ligase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.800:FF:000004">
        <property type="entry name" value="Glycine--tRNA ligase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000010">
        <property type="entry name" value="Glycyl-tRNA synthetase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.720.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.10">
        <property type="entry name" value="S15/NS1, RNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027031">
        <property type="entry name" value="Gly-tRNA_synthase/POLG2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033731">
        <property type="entry name" value="GlyRS-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002315">
        <property type="entry name" value="tRNA-synt_gly"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009068">
        <property type="entry name" value="uS15_NS1_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000738">
        <property type="entry name" value="WHEP-TRS_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00389">
        <property type="entry name" value="glyS_dimeric"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003211">
        <property type="entry name" value="PRK04173.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10745:SF0">
        <property type="entry name" value="GLYCINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10745">
        <property type="entry name" value="GLYCYL-TRNA SYNTHETASE/DNA POLYMERASE SUBUNIT GAMMA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00458">
        <property type="entry name" value="WHEP-TRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01043">
        <property type="entry name" value="TRNASYNTHGLY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00991">
        <property type="entry name" value="WHEP-TRS"/>
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        <property type="entry name" value="Class II aaRS ABD-related"/>
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    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF47060">
        <property type="entry name" value="S15/NS1 RNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
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    </dbReference>
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        <property type="entry name" value="WHEP_TRS_1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="WHEP_TRS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0436">Ligase</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
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    <keyword evidence="8" id="KW-0808">Transferase</keyword>
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            <begin position="9"/>
            <end position="65"/>
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    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="17">
        <location>
            <begin position="187"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="34"/>
            <end position="61"/>
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    </feature>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00012829"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00030057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000018157.4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="675" mass="76436" checksum="338CB9A9DBCEFE30" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>F6QEE6</accession>
    <name>F6QEE6_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Neutral ceramidase</fullName>
            <ecNumber evidence="2 7">3.5.1.23</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">wild type</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <reaction evidence="12 7">
            <text>an N-acylsphing-4-enine + H2O = sphing-4-enine + a fatty acid</text>
            <dbReference type="Rhea" id="RHEA:20856"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:52639"/>
            <dbReference type="ChEBI" id="CHEBI:57756"/>
            <dbReference type="EC" id="3.5.1.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="6">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the neutral ceramidase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.23" evidence="2 7"/>
    <dbReference type="EMBL" id="EAAA01000946">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6QEE6"/>
    <dbReference type="STRING" id="7719.ENSCINP00000006462"/>
    <dbReference type="Ensembl" id="ENSCINT00000006462.3">
        <property type="protein sequence ID" value="ENSCINP00000006462.3"/>
        <property type="gene ID" value="ENSCING00000003155.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015792"/>
    <dbReference type="HOGENOM" id="CLU_011300_2_0_1"/>
    <dbReference type="InParanoid" id="F6QEE6"/>
    <dbReference type="OMA" id="EYTTMAG"/>
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        <property type="component" value="Chromosome 12"/>
    </dbReference>
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        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017040">
        <property type="term" value="F:N-acylsphingosine amidohydrolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046514">
        <property type="term" value="P:ceramide catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="P:long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="P:sphingosine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Neutral/alkaline non-lysosomal ceramidase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006823">
        <property type="entry name" value="Ceramidase_alk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038445">
        <property type="entry name" value="NCDase_C_sf"/>
    </dbReference>
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        <property type="entry name" value="NEUT/ALK_ceramidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031329">
        <property type="entry name" value="NEUT/ALK_ceramidase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12670">
        <property type="entry name" value="CERAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12670:SF1">
        <property type="entry name" value="NEUTRAL CERAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ceramidase_alk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17048">
        <property type="entry name" value="Ceramidse_alk_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Neutral/alkaline non-lysosomal ceramidase N-terminal" evidence="8">
        <location>
            <begin position="9"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="domain" description="Neutral/alkaline non-lysosomal ceramidase C-terminal" evidence="9">
        <location>
            <begin position="584"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="317"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="512"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="552"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR606823-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR606823-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF17048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000006462.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="748" mass="82474" checksum="8E0EFE287F1B55B2" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>F6QEL1</accession>
    <accession>A0A1W2WD82</accession>
    <name>F6QEL1_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized LOC100176714</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100176714</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Tunicata</taxon>
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            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
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                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
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                <person name="Hastings K.E."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
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<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
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                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
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                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="5">wild type</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
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                <person name="Kawashima T."/>
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                <person name="Martinez D."/>
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                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
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                <person name="Mochizuki Y."/>
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                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
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                <person name="Zhang C."/>
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                <person name="Doggett N."/>
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                <person name="Hawkins T."/>
                <person name="Richardson P."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
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                <person name="Shoguchi E."/>
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                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
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                <person name="Wiley G.B."/>
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                <person name="Roe B.A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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                <person name="Satou Y."/>
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                <person name="Kobayashi M."/>
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                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
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                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
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                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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    <reference evidence="3" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
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        <location>
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    <evidence type="ECO:0000256" key="1">
        <source>
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    </evidence>
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        <source>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
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                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
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                <person name="Tassy O."/>
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                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
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                <person name="Wiley G.B."/>
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                <person name="Roe B.A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">wild type</strain>
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        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
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                <person name="De Tomaso A."/>
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                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
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                <person name="Kobayashi K."/>
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                <person name="Lee B.I."/>
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                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
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                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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    <reference evidence="13" key="3">
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <text evidence="1">Belongs to the centaurin gamma-like family.</text>
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<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
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        <name type="scientific">Ciona intestinalis</name>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
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                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
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                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
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                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
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                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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                <person name="Hotta K."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
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    <reference evidence="3" key="3">
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                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
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                <person name="Terry A."/>
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                <person name="Detter C."/>
                <person name="Doggett N."/>
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                <person name="Richardson P."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">wild type</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
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                <person name="Awazu S."/>
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                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
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                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="De Tomaso A."/>
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                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
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                <person name="Meinertzhagen I.A."/>
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                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
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                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
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                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
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                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
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                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
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                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
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                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="11" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">wild type</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
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    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="F6TDK9"/>
    <dbReference type="Ensembl" id="ENSCINT00000013663.3">
        <property type="protein sequence ID" value="ENSCINP00000013663.3"/>
        <property type="gene ID" value="ENSCING00000020012.1"/>
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    <dbReference type="HOGENOM" id="CLU_1874718_0_0_1"/>
    <dbReference type="InParanoid" id="F6TDK9"/>
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        <property type="entry name" value="LEUCOKININ RECEPTOR"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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            <dbReference type="PROSITE" id="PS50262"/>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000013663.3"/>
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        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="136" mass="15064" checksum="A85C1450AA64E5A5" modified="2012-04-18" version="2">MRMITTMVGVFTVCVCPYVIVTGLMFNGTLNCNNSGPLYNVAIYLVVMNSAMNFFIYNFWYKNFRQQFRAVFQTTAERRRNAGKASVAATSASRSRTSTGNWSSLAKGSATRSVEYKGGVTSFLQPAPVSNQKLPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
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    <name>F6TIU3_CIOIN</name>
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        <recommendedName>
            <fullName evidence="8">Eukaryotic translation initiation factor 3 subunit I</fullName>
            <shortName evidence="8">eIF3i</shortName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">wild type</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.</text>
    </comment>
    <comment type="function">
        <text evidence="7">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the WD repeat STRAP family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the eIF-3 subunit I family.</text>
    </comment>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000007786"/>
    <dbReference type="Ensembl" id="ENSCINT00000007786.3">
        <property type="protein sequence ID" value="ENSCINP00000007786.3"/>
        <property type="gene ID" value="ENSCING00000003743.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161371"/>
    <dbReference type="HOGENOM" id="CLU_043845_0_1_1"/>
    <dbReference type="InParanoid" id="F6TIU3"/>
    <dbReference type="OMA" id="VWFSHNG"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071541">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex, eIF3m"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002183">
        <property type="term" value="P:cytoplasmic translational initiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="entry name" value="Eukaryotic translation initiation factor 3 subunit I"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03008">
        <property type="entry name" value="eIF3i"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027525">
        <property type="entry name" value="eIF3i"/>
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        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
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        <property type="entry name" value="WD40_repeat_CS"/>
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        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
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    <dbReference type="PANTHER" id="PTHR19877">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19877:SF1">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24805">
        <property type="entry name" value="EIF3I"/>
        <property type="match status" value="1"/>
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    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
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        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
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        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 8" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5 8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 9" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="6"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="48"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="185"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="282"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00057041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000007786.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="326" mass="36793" checksum="5BE51E579425BDF2" modified="2012-04-18" version="2">MRPLMLQGHERAITQIRYNREGDLLFTVAKDATPNVWYTINGERLGTYDGHRGTVWSVDVNWDSTKVLTGAADLSCKLWDCETGKVINTWDTKSSVRSSNFSYCGNMINFTTDRQMKHEPKICIYDIREPSDSEPALSFVIDTKEMSKPMCSLWGALNEYLITGHDDGTMAHWDLKVGGELLHSVREHTALIKDMQLSSDQMMLITASKDKTAKLFDAPSLNLMKTYKTDRPVNSAAISPIRDHVVLGGGQEAMDVTTSNLKEGKFDARFFHLVFEEEFGRVKGHFGPINSLAFHPNGNSYSSGGEDGFVRVHQFDESYFDFQFEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>F6TSI9</accession>
    <accession>A0A1W5BKS0</accession>
    <name>F6TSI9_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Beta-secretase 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC108950261</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the peptidase A1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_018671056.1">
        <property type="nucleotide sequence ID" value="XM_018815511.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6TSI9"/>
    <dbReference type="STRING" id="7719.ENSCINP00000008898"/>
    <dbReference type="Ensembl" id="ENSCINT00000008898.3">
        <property type="protein sequence ID" value="ENSCINP00000008898.3"/>
        <property type="gene ID" value="ENSCING00000004309.3"/>
    </dbReference>
    <dbReference type="GeneID" id="108950261"/>
    <dbReference type="KEGG" id="cin:108950261"/>
    <dbReference type="HOGENOM" id="CLU_039009_0_0_1"/>
    <dbReference type="InParanoid" id="F6TSI9"/>
    <dbReference type="OMA" id="CYKFAIF"/>
    <dbReference type="OrthoDB" id="2747330at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050435">
        <property type="term" value="P:amyloid-beta metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006509">
        <property type="term" value="P:membrane protein ectodomain proteolysis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000007">
        <property type="entry name" value="Beta-secretase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009119">
        <property type="entry name" value="BACE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47965:SF12">
        <property type="entry name" value="ASPARTIC PROTEINASE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47965">
        <property type="entry name" value="ASPARTYL PROTEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01815">
        <property type="entry name" value="BACEFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="7 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 12" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0732">Signal</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5015091094" evidence="14">
        <location>
            <begin position="21"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="421"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="15">
        <location>
            <begin position="44"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="62"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000008898.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="466" mass="51856" checksum="EB2E1D6F5349FA48" modified="2011-07-27" version="1">MEFNFGNLLFMAVLLCMGDAKIRIPLRYKRASANNLKGTTVTGYYITVNLGTPPQTFNVIVDTGSSNFAVSGTKTSFSDSFYNKALSNTSIDTTLPTVDVHYTEGWWKGNIVSDLLTIPSANLIQPVRVHVADISETKNFFINESQWIGILGLAYSSLVLPKNGGLWSVMHDIVEQTTLEDILSMQLCSTPTRDETQGVLLFGEWDSSLATGQIYWTRIVKQWYYDILITGLKVGDTMIEVDCEELNNDRTIVDSGTTNLRLPQKVYNLVVSVIKTKLVKDDFDDDFYDGTKMFCTSEPNVLFSWFPNLSIFLPSTEANKTIELTVFPQSYLQLTEGLVTETLLGRSCYKFAIFPSTSGSVLGTVVMEQYYVVFDRGNNTVGFATSKCAVLPTTSIQIRNGWINSTSCQYISHSVKSPLTLAAYIVLGLFCICLLPVCFLIAYNFFRKRSPGIGCCSGSYNQLNDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>F6TX28</accession>
    <name>F6TX28_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01001178">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6TX28"/>
    <dbReference type="FunCoup" id="F6TX28">
        <property type="interactions" value="17"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000010331"/>
    <dbReference type="Ensembl" id="ENSCINT00000010331.3">
        <property type="protein sequence ID" value="ENSCINP00000010331.3"/>
        <property type="gene ID" value="ENSCING00000005026.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000004952"/>
    <dbReference type="HOGENOM" id="CLU_151471_0_0_1"/>
    <dbReference type="InParanoid" id="F6TX28"/>
    <dbReference type="OMA" id="RTHEFQF"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
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        <property type="entry name" value="Ubiquitin-like_dom"/>
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        <property type="entry name" value="UBL3-like"/>
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        <property type="entry name" value="UBL3-like_ubiquitin_dom"/>
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        <property type="entry name" value="UBIQUITIN-LIKE PROTEIN 3"/>
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        <property type="entry name" value="UBIQUITIN-LIKE PROTEIN 3 HCG-1 PROTEIN"/>
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        <property type="entry name" value="Rad60-SLD_2"/>
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        <property type="entry name" value="UBIQUITIN_2"/>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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            <begin position="22"/>
            <end position="105"/>
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    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    </evidence>
    <sequence length="114" mass="12663" checksum="75CAC1E81B9FBEE6" modified="2012-04-18" version="2">MENEMTETAKLNQPAVPNKDQVGLKLLRISGATHSFVFLPSDTISDVTKHVFDNWPEEWTEDTVEEHGVLRLIYQGRFLHGKATLGALKIPSGKTTIMHLVSRATVPEPNAQGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>F6UDJ0</accession>
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        <recommendedName>
            <fullName evidence="6">S1 motif domain-containing protein</fullName>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
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            <taxon>Ciona</taxon>
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    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
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        <property type="protein sequence ID" value="ENSCINP00000005538.3"/>
        <property type="gene ID" value="ENSCING00000002715.3"/>
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    <dbReference type="GeneTree" id="ENSGT00390000012228"/>
    <dbReference type="HOGENOM" id="CLU_000845_0_0_1"/>
    <dbReference type="InParanoid" id="F6UDJ0"/>
    <dbReference type="OMA" id="GQYLRAY"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="project" value="GO_Central"/>
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        <property type="term" value="C:small-subunit processome"/>
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        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Programmed cell death 11"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="protein RRP5 homolog"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Nucleic acid-binding proteins"/>
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    </dbReference>
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        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Rrp5"/>
    </dbReference>
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        <property type="entry name" value="Rrp5_S1"/>
    </dbReference>
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        <property type="entry name" value="Suf"/>
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        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR23270:SF10">
        <property type="entry name" value="PROTEIN RRP5 HOMOLOG"/>
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    <dbReference type="Pfam" id="PF00575">
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        <location>
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    <feature type="domain" description="S1 motif" evidence="6">
        <location>
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        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="6">
        <location>
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        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="6">
        <location>
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    </feature>
    <feature type="domain" description="S1 motif" evidence="6">
        <location>
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    </feature>
    <feature type="domain" description="S1 motif" evidence="6">
        <location>
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            <end position="1072"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="6">
        <location>
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    </feature>
    <feature type="domain" description="S1 motif" evidence="6">
        <location>
            <begin position="1289"/>
            <end position="1361"/>
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    </feature>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
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        <source>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>F6UG00</accession>
    <name>F6UG00_CIOIN</name>
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        <recommendedName>
            <fullName evidence="6">Protein unc-93 homolog A</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
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            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">wild type</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the unc-93 family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002943">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6UG00"/>
    <dbReference type="STRING" id="7719.ENSCINP00000024077"/>
    <dbReference type="Ensembl" id="ENSCINT00000024323.1">
        <property type="protein sequence ID" value="ENSCINP00000024077.1"/>
        <property type="gene ID" value="ENSCING00000013026.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063359"/>
    <dbReference type="HOGENOM" id="CLU_089764_1_0_1"/>
    <dbReference type="InParanoid" id="F6UG00"/>
    <dbReference type="OMA" id="ANCALMN"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010291">
        <property type="entry name" value="Ion_channel_UNC-93"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051951">
        <property type="entry name" value="UNC-93_regulatory"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19444:SF13">
        <property type="entry name" value="PROTEIN UNC-93 HOMOLOG A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19444">
        <property type="entry name" value="UNC-93 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05978">
        <property type="entry name" value="UNC-93"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="49"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="194"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00040854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000024077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="224" mass="24589" checksum="4BBC157FA54CBE4D" modified="2011-07-27" version="1">MVTSANCALMNLQTSMNVEGGLGALGSTVMTSTLLVSTLTIVPVISKYLDVKVFLVSTQLMFIVYVAANFYPAYYTILPASSITGFAKSAFWVCVGKYNLQFAKKYNLIGSGSLSKYINYVTGYFFCAYQSCQVIGNMIPFVIFKVAPQNENMTHSHLLNSSRILVSEACGANDCQLPSQEDPSKYRQAEQTTIYIMLGVFLVMTFVGAISYMFIPSPANGEFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>F6UL80</accession>
    <name>F6UL80_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">wild type</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="EAAA01002712">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6UL80"/>
    <dbReference type="STRING" id="7719.ENSCINP00000015189"/>
    <dbReference type="Ensembl" id="ENSCINT00000015189.3">
        <property type="protein sequence ID" value="ENSCINP00000015189.3"/>
        <property type="gene ID" value="ENSCING00000007395.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165452"/>
    <dbReference type="HOGENOM" id="CLU_971564_0_0_1"/>
    <dbReference type="InParanoid" id="F6UL80"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050394">
        <property type="entry name" value="Homeobox_NK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24340:SF73">
        <property type="entry name" value="HOMEOBOX PROTEIN BAGPIPE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24340">
        <property type="entry name" value="HOMEOBOX PROTEIN NKX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 5 6" id="KW-0371">Homeobox</keyword>
    <keyword evidence="4 5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="8">
        <location>
            <begin position="153"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="5">
        <location>
            <begin position="155"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="92"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="266"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="92"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="126"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="271"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000015189.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="287" mass="32985" checksum="912A2576FD768D44" modified="2012-04-18" version="2">QLPNNKNTPSGSSPPYWNITTKPHKIWRPIDPDDPARPPNYFPMFSGYPYNSAYASNHQQCLEKLFAENYLLHSQRVKDIYAANTSRTAFQTRQFDHTSMQNADSSAQRLQTPESPEMNKPDETASRSVQSSPANCLSPRTENIDILKTKKRTTRKRTRAAFTQAQVSALEQRFIQQKYLTGNERTEFAHALQLTETQIKIWFQNRRYKTKRRLRLASEMWAAAAQQAQRAAFALGVPTGMCYPNLIPAYCGKLAPLRLQSFPEPLPFSPPNRSRMSNEMFTDKNNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>F6USW1</accession>
    <name>F6USW1_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
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                <person name="Mount S."/>
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                <person name="Nomoto H."/>
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                <person name="Rigoutsos I."/>
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                <person name="Sasaki A."/>
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                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1">wild type</strain>
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    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="1" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    <sequence length="114" mass="12633" checksum="E2F4A1BE0E681FB2" modified="2012-04-18" version="2">ACEAISKLSDLRSVILALLSASDTRTLLETVRLLRTCLADQKSSNLWVETAEENVKDLHENSIFILSCSTNGKLLSSLSEVLDQLFKLSPEKVLEKFSTKEFVASLLEALGQLY</sequence>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
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                <person name="Di Gregorio A."/>
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                <person name="Kawashima T."/>
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                <person name="Yoshizaki F."/>
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                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
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                <person name="Richardson P."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="8" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Ogasawara M."/>
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                <person name="Yamada L."/>
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                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
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                <person name="Hastings K.E."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="8">wild type</strain>
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    <reference evidence="8" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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            <topology evidence="1">Multi-pass membrane protein</topology>
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        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045263">
        <property type="entry name" value="GLUT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23503:SF8">
        <property type="entry name" value="FACILITATED GLUCOSE TRANSPORTER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23503">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="58"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="94"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="118"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="152"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="183"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="11"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000028051.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="263" mass="29258" checksum="F91413230AB18A55" modified="2012-04-18" version="2">MENSNRANVSLLMHGTMLMFASSYLFVLVNPLNSIIRTIYNETYMERNGVAMSKSTWAVYLSLTSSFFIIGGLVGGLGMQFILKITSRKRLMQLAHVINILSVILIAGVGKITESYEVFIVGRFVGGICFGLSFNLPSLMVSETSIKQKQNFWQSIISVSSCCGILFAVIIGHDKILGSLTLWPVAFSIGALPSIVYLITSLHLPNTPYDVLHRLGKQEALNVLRTLRNGSKELIHQEFELLQPDIELTRKIGIIEILSIKQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>F6W804</accession>
    <name>F6W804_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phospholipid/glycerol acyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">wild type</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001428">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6W804"/>
    <dbReference type="FunCoup" id="F6W804">
        <property type="interactions" value="20"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000021982"/>
    <dbReference type="Ensembl" id="ENSCINT00000022228.2">
        <property type="protein sequence ID" value="ENSCINP00000021982.2"/>
        <property type="gene ID" value="ENSCING00000011528.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182836"/>
    <dbReference type="HOGENOM" id="CLU_041844_2_0_1"/>
    <dbReference type="InParanoid" id="F6W804"/>
    <dbReference type="OMA" id="WEILASE"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036149">
        <property type="term" value="P:phosphatidylinositol acyl-chain remodeling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07990">
        <property type="entry name" value="LPLAT_LCLAT1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032098">
        <property type="entry name" value="Acyltransf_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10983">
        <property type="entry name" value="1-ACYLGLYCEROL-3-PHOSPHATE ACYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10983:SF16">
        <property type="entry name" value="LYSOCARDIOLIPIN ACYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16076">
        <property type="entry name" value="Acyltransf_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="3" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phospholipid/glycerol acyltransferase" evidence="5">
        <location>
            <begin position="77"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000021982.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="281" mass="32675" checksum="E08BBBDD0E87C6C3" modified="2012-04-18" version="2">IPDSRVEVPFGNLSSIVMSSIVTDCWVLATNFNKNVYTAITRIVQHLVFSPYSTLVLNYAGMKVVVTGDAIQRHEKTMVLMNHRTRLDWLYFFPYVFHARILNRQKIALKSMLKWIPGLGWAMQVAGYIFLDRQWEADQVHISNILSYFVELESKPNILFFAEGTDFNEGSKKRSKEYARKSGLTEFEYVLQPRTTGFTYFVNHLRNISGIHAVHDVTIAYPYEILHNELELIKAGAPRAVHFHIKRYSISELPEDQDELGKWCQNLWAEKEALLKEYYSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>F6WG20</accession>
    <accession>A0A1W2W4V5</accession>
    <name>F6WG20_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Solute carrier family 35 member G1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100177875</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_002120287.1">
        <property type="nucleotide sequence ID" value="XM_002120251.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6WG20"/>
    <dbReference type="FunCoup" id="F6WG20">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSCINT00000000302.3">
        <property type="protein sequence ID" value="ENSCINP00000000302.3"/>
        <property type="gene ID" value="ENSCING00000000177.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100177875"/>
    <dbReference type="KEGG" id="cin:100177875"/>
    <dbReference type="GeneTree" id="ENSGT00940000153249"/>
    <dbReference type="HOGENOM" id="CLU_032828_1_2_1"/>
    <dbReference type="InParanoid" id="F6WG20"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="evidence" value="ECO:0000318"/>
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    <dbReference type="PANTHER" id="PTHR22911">
        <property type="entry name" value="ACYL-MALONYL CONDENSING ENZYME-RELATED"/>
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    </dbReference>
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        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER G1"/>
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    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="37"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
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    </feature>
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    <feature type="domain" description="EamA" evidence="6">
        <location>
            <begin position="40"/>
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        <location>
            <begin position="193"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000000302.3"/>
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    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    </evidence>
    <sequence length="343" mass="37859" checksum="5482EAD0B2E39E3D" modified="2011-07-27" version="1">MESTKRNEDISLAESCSDSDVINHNEKHKSAKPKPPFMGFGMLCCILTSVLFAVNALMVKMVKSIGPIEVLGARCWVQFFMLLPFITYNWMHNKVDFLGPRNTFKLLCLRSLTGSTAAMFLYQSLQRLPLGDAVTISFLSLVFTMLFAAVFLNERPTVLDIIFSIVIIAGVVLVAQPPFLFDSSVTYDTERLYGTIFGILCSAMAGVTFVIVRKLGIATHATLNVFYYSFAGSITSLIFTTTIETFKFPCSSEIQYIIFAGIAGCGAQIFMALALQYERAGTFSMLKSFQIILSFAFQVIILQDIPSSLSLGGAALVFASIFGIAVRKIYNEYFKGPKLGCKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>F6WVN4</accession>
    <name>F6WVN4_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Phosphatidic acid phosphatase type 2/haloperoxidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">wild type</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the type 2 lipid phosphate phosphatase family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01000486">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6WVN4"/>
    <dbReference type="STRING" id="7719.ENSCINP00000026500"/>
    <dbReference type="Ensembl" id="ENSCINT00000026746.2">
        <property type="protein sequence ID" value="ENSCINP00000026500.2"/>
        <property type="gene ID" value="ENSCING00000014732.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000169714"/>
    <dbReference type="HOGENOM" id="CLU_043042_1_0_1"/>
    <dbReference type="InParanoid" id="F6WVN4"/>
    <dbReference type="OMA" id="GEARWNT"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042392">
        <property type="term" value="F:sphingosine-1-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046839">
        <property type="term" value="P:phospholipid dephosphorylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006670">
        <property type="term" value="P:sphingosine metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.144.10">
        <property type="entry name" value="Phosphatidic acid phosphatase type 2/haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14969:SF28">
        <property type="entry name" value="DIHYDROSPHINGOSINE 1-PHOSPHATE PHOSPHATASE LCB3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14969">
        <property type="entry name" value="SPHINGOSINE-1-PHOSPHATE PHOSPHOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="51"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="126"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="153"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="184"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="211"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="9">
        <location>
            <begin position="84"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000026500.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="302" mass="33559" checksum="8552B20C8C77E8E7" modified="2012-04-18" version="2">MNIVSTLNSPVPIAKFQYICGVRWTNPDPKNVIYPNGDLGKEKGYIVMRKSFYWLATAGSELAGECFYYIFFPSCAWVFNLGVARRSLLFLSTSMLLGQSLKDVLKLPRPSSPPVIRLEATYETEYGMPSTHTIAAIAVSFSLLLFTSDIYKINYASGLTAATMWSLIVMLSRIYLGMHSLLDIAAGASFVGFLLSVLPRAIYWFDSTQLASSHLPTCVFGACTIFVSWLVFQLDSKVDKKTGEARWNTARGDTAEILGWCLGFMVGSRYSILNGILPKTSISTLTQSFKLPSYNMLVRFLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>F6WXQ7</accession>
    <name>F6WXQ7_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">wild type</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002816">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6WXQ7"/>
    <dbReference type="STRING" id="7719.ENSCINP00000007073"/>
    <dbReference type="Ensembl" id="ENSCINT00000007073.3">
        <property type="protein sequence ID" value="ENSCINP00000007073.3"/>
        <property type="gene ID" value="ENSCING00000003453.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160689"/>
    <dbReference type="HOGENOM" id="CLU_1264089_0_0_1"/>
    <dbReference type="InParanoid" id="F6WXQ7"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016712">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008210">
        <property type="term" value="P:estrogen metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006706">
        <property type="term" value="P:steroid catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009404">
        <property type="term" value="P:toxin metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042178">
        <property type="term" value="P:xenobiotic catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24289">
        <property type="entry name" value="STEROID 17-ALPHA-HYDROXYLASE/17,20 LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24289:SF1">
        <property type="entry name" value="STEROID 17-ALPHA-HYDROXYLASE_17,20 LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0349">Heme</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000007073.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="243" mass="27341" checksum="DEE5F7D1FDB9348E" modified="2012-04-18" version="2">MYDVIIVCVISAIAVVAASWVKQKGSKKFPPGSRGLPFLGSVPFVLGSLERTLAVWGWQNYGPVFFFTRGNTRTVVLNTYEAASEGFVKQGNHLSGRFIDANHNELFADYSPQWQEQRKLRVKTLKGFGKVNTEEIMTSECKKMICRIRETKGSFEAGYYLAVPTFNIVKSIIYGEPSEGMNKQDEVNLSKVLADPVYKAGNGFMEFMAGMFPQIRSSVAYERMLEKKGKERVRQVKEKMTGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>F6X4N7</accession>
    <name>F6X4N7_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="5">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the PPP phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="F6X4N7"/>
    <dbReference type="FunCoup" id="F6X4N7">
        <property type="interactions" value="1112"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000027923"/>
    <dbReference type="Ensembl" id="ENSCINT00000028169.2">
        <property type="protein sequence ID" value="ENSCINP00000027923.2"/>
        <property type="gene ID" value="ENSCING00000015948.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158785"/>
    <dbReference type="HOGENOM" id="CLU_004962_0_5_1"/>
    <dbReference type="InParanoid" id="F6X4N7"/>
    <dbReference type="OMA" id="HESSSMN"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07417">
        <property type="entry name" value="MPP_PP5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000017">
        <property type="entry name" value="Serine/threonine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041753">
        <property type="entry name" value="PP5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051134">
        <property type="entry name" value="PPP_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45668">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45668:SF5">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0464">Manganese</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="6">
        <location>
            <begin position="124"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000027923.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="324" mass="37000" checksum="1A4E05ACDD62B172" modified="2012-04-18" version="2">KKLVSESINLDNIWSRHLKIRISYTEVTNLYSTFRYAYQILLEMKEILSGLPSLVDIDDQLTVCGDVHGQYYDMCHVFELNGLPAEDNPYLFNGDFVDRGSWSVEVILVLFGYKILLPNKFHLVRGNHETDYMNQMYGFEGEVKAKYGGNMMGLFSEVFNWLPLSYCINGKVLIMHGGLCEKDGVTLDDLRKIERNRQPPENGLMCDLLWSDPQAKMGRGVSKRGVSCQFGPDITSNFLRNNGLDYIIRSHECKNGGYEEQHNGKCITIFSAPNYCDQMGNKGAFITLRAPDLIPKFTQFEAVKHPEVQPMAYANSFMRMNGMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>F6X646</accession>
    <accession>A0A1W2W8I6</accession>
    <name>F6X646_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized LOC100176540</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC100176540</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01002493">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002126556.1">
        <property type="nucleotide sequence ID" value="XM_002126520.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6X646"/>
    <dbReference type="FunCoup" id="F6X646">
        <property type="interactions" value="28"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000003614"/>
    <dbReference type="Ensembl" id="ENSCINT00000003614.3">
        <property type="protein sequence ID" value="ENSCINP00000003614.3"/>
        <property type="gene ID" value="ENSCING00000001793.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100176540"/>
    <dbReference type="KEGG" id="cin:100176540"/>
    <dbReference type="GeneTree" id="ENSGT00390000017397"/>
    <dbReference type="HOGENOM" id="CLU_834073_0_0_1"/>
    <dbReference type="InParanoid" id="F6X646"/>
    <dbReference type="OMA" id="NINLNWG"/>
    <dbReference type="OrthoDB" id="9992964at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904262">
        <property type="term" value="P:negative regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048011">
        <property type="term" value="P:neurotrophin TRK receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026682">
        <property type="entry name" value="AKT1S1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21844">
        <property type="entry name" value="AKT1 SUBSTRATE 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21844:SF2">
        <property type="entry name" value="PROLINE-RICH AKT1 SUBSTRATE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="128"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="151"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="161"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="178"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000003614.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="333" mass="36993" checksum="B26DEF6CFA777001" modified="2012-04-18" version="2">MESAMGTTQLQAWKSLKLAVDKYSQSTGAEVAVATTYFADTTDNHLSFNQSKPKTTVYGHGSLLFEVRKIVEVPGASMRDQQWIIPEKNVDNADVINAQQIPINPKLHQQSVSRSLASSFRTLKLDSHPSTLAHGSSGSACITPPSMQDSPAHEIKDKPGTGRRKSKQKKSKKQNLSEDFELDDNLLFDEHDEEPSSDDESEDSDESEEDPRPLRFGRYHAPPSGRMLQQNLLSQDQFATSLPVNINLNWGNRTDASRFRANQGPHPPTFDQSKLVTSGPKIVRADKTPQLDTMMASMQALARSVTDDSALIFGDRPRPRIHDESGFKGNMKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>F6X9E5</accession>
    <accession>A0A1W2W998</accession>
    <name>F6X9E5_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Methanethiol oxidase</fullName>
            <ecNumber evidence="3">1.8.3.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100177120</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>methanethiol + O2 + H2O = hydrogen sulfide + formaldehyde + H2O2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11812"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16007"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="EC" id="1.8.3.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Organosulfur degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the selenium-binding protein family.</text>
    </comment>
    <dbReference type="EC" id="1.8.3.4" evidence="3"/>
    <dbReference type="RefSeq" id="XP_002126964.1">
        <property type="nucleotide sequence ID" value="XM_002126928.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6X9E5"/>
    <dbReference type="FunCoup" id="F6X9E5">
        <property type="interactions" value="91"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000010226"/>
    <dbReference type="Ensembl" id="ENSCINT00000010226.3">
        <property type="protein sequence ID" value="ENSCINP00000010226.3"/>
        <property type="gene ID" value="ENSCING00000004957.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100177120"/>
    <dbReference type="KEGG" id="cin:100177120"/>
    <dbReference type="GeneTree" id="ENSGT00390000014244"/>
    <dbReference type="HOGENOM" id="CLU_032512_0_0_1"/>
    <dbReference type="InParanoid" id="F6X9E5"/>
    <dbReference type="OMA" id="AYDFWWH"/>
    <dbReference type="OrthoDB" id="10252446at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018549">
        <property type="term" value="F:methanethiol oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008430">
        <property type="term" value="F:selenium binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008826">
        <property type="entry name" value="Se-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23300">
        <property type="entry name" value="METHANETHIOL OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23300:SF0">
        <property type="entry name" value="METHANETHIOL OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05694">
        <property type="entry name" value="SBP56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75011">
        <property type="entry name" value="3-carboxy-cis,cis-mucoante lactonizing enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0711">Selenium</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000010226.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="486" mass="54251" checksum="0A965F4F38118944" modified="2012-04-18" version="2">MSNRVEAISKHLESSCCKSATGPGYRSPIDAMKAPKEKLLYIPCIYRNTPISQPDYLATIDMDESSPTFCQVIDRLDLPNMNDEIHHTGWNSCSSCFHDPSKKRRLFILPGLVSSRLYAVDVDTDPKKPRIEKVVEPTEIFHRTGLANPHTVHCIPGGQIMISTIGDPSGNGKGGFLILDSETFELIGNWEAPGHNTPFGYDFWYQPRHNVMISTEWGHPKKLLKEFNPDDAAAGHYGHCLHVWDWEKHELIQDIDLGPNGMIPLEIRFLHDPSATEGFVGCALSSTVFRFFKTKKGDWDAEQVIAIPPKKVEGWALPDMPGLITDIILSMDDRFLYFSNWLHGDIRQYDVTDTKNPKLVGQIFLGGSICTDGGVKVTKDTELKSQPAPAFIGGKKIQGGPQMLQLSLDGKRLYVTSSLYSGWDKQFYPDMMNSGSHLLQIDVNTETGGLSLNRNVFVDFGNEPKGPVLAHEIRYPGGDCTSDIWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F6XZD2</accession>
    <name>F6XZD2_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="F6XZD2"/>
    <dbReference type="Ensembl" id="ENSCINT00000027284.2">
        <property type="protein sequence ID" value="ENSCINP00000027038.2"/>
        <property type="gene ID" value="ENSCING00000015151.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015321"/>
    <dbReference type="HOGENOM" id="CLU_649806_0_0_1"/>
    <dbReference type="InParanoid" id="F6XZD2"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="319"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="319"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="334"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000027038.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="423" mass="45954" checksum="84CDAE2A8CC959E5" modified="2012-04-18" version="2">MHTMNADEYTSILAKEISQEIENQSFDYNTSQSTTSNMENCTESSKSLPFETICCAGDTRCSSHNVTSSKPTSIMTSQLIALDNTKFKLSAQPMKVLPHTQQLNTKAKQKNTKQTIKRSKLKTTRKQNMVKQETSTSCTKQSSSVNSSVAVLTHVQSAVKSKIDKQRAIGKKIRYNQAVHSNIIPVAQPVVALTPTVRVQPHIMPSNVVCTTGKLSNNNQSMVLIPVNAYVRPGNNMSQSMGLAFAVPAHNLTSNVGTVIPIRNSTVMTGACPQQLIILNGSQAGIATCANMAPSKQTINTTVYPPNVTNTIITQQSSVKKPTTTTTQSKSILLKNNQTRNMNVSSPVTTSNRNYQINNNQLQSITLTSPTNNTLVFSSNDLTMTNATSTNQVTSTSVSTCSSTIMINTSNQSRIQTLVDEHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>F6YD46</accession>
    <name>F6YD46_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">catechol O-methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.6</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-dependent O-methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.6" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="F6YD46"/>
    <dbReference type="FunCoup" id="F6YD46">
        <property type="interactions" value="71"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000005439"/>
    <dbReference type="Ensembl" id="ENSCINT00000005439.3">
        <property type="protein sequence ID" value="ENSCINP00000005439.3"/>
        <property type="gene ID" value="ENSCING00000002670.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000167509"/>
    <dbReference type="HOGENOM" id="CLU_050461_5_0_1"/>
    <dbReference type="InParanoid" id="F6YD46"/>
    <dbReference type="OMA" id="IGYSAIM"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016206">
        <property type="term" value="F:catechol O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042424">
        <property type="term" value="P:catecholamine catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032502">
        <property type="term" value="P:developmental process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042417">
        <property type="term" value="P:dopamine metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000054">
        <property type="entry name" value="Catechol O-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002935">
        <property type="entry name" value="SAM_O-MeTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43836">
        <property type="entry name" value="CATECHOL O-METHYLTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43836:SF2">
        <property type="entry name" value="CATECHOL O-METHYLTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01596">
        <property type="entry name" value="Methyltransf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51682">
        <property type="entry name" value="SAM_OMT_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0128">Catecholamine metabolism</keyword>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-0531">Neurotransmitter degradation</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000005439.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="268" mass="30422" checksum="3FE8D891E90EF015" modified="2012-04-18" version="2">ETRAFFSHCCGGFCAGKPLLSYTAVPRCLSLHKWRLYLFRLAVVVDHIRIMAKGTDLTFPYHIVDHVVKTAKRGDVSSVIDVIDQYCYSVEKAMNVGDVKGAILDKCFLEASPQNVLELGCYCGYSSLRMSRLLKEDAKIFTIEANPDYAKFAQQIITFAGMEKRIQILQGLSDEVLPTIAEKCGVQTFDFVFIDHAKELYKRDLIMLENLGLIRHGTTIVADNVIYPGCPDYLKYVRNNPKYKSANYLSKLEYTDREDAIEKSIYLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>F6YDR3</accession>
    <name>F6YDR3_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF3517 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01002394">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6YDR3"/>
    <dbReference type="STRING" id="7719.ENSCINP00000005400"/>
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                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="ENSCING00000012408.2"/>
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        <property type="component" value="Chromosome 1"/>
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    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
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        <property type="entry name" value="Auxin_biosynth/cation_transpt"/>
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        <property type="entry name" value="FAD/NAD-bd_sf"/>
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        <property type="entry name" value="FAD-DEPENDENT OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="FLAVIN-BINDING MONOOXYGENASE-LIKE PROTEIN (AFU_ORTHOLOGUE AFUA_4G09220)"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Pyr_redox_3"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
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    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
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        <location>
            <begin position="1"/>
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    </feature>
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            <begin position="20"/>
            <end position="248"/>
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    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
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        <source>
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        <source>
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    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    </evidence>
    <sequence length="248" mass="28366" checksum="81B854275ED84711" modified="2012-04-18" version="2">MQVSVFVLVLFCLCLSVYGKNKHEGSIKHDDPMLNTETPNYKEYCVIGAGPGGLQIAYYLESSERDYIVFEKGHGAGTFYNKFPIHRKLISLNKVYTGKTNKEFNMRHDWNSLLSNDESLLMTKYSEEFFPPADVLVQYLNDYATKLNLKVQYNSTMHDILKRKDGLFQMWDQHNNAYTCKYVIVATGIAKPNHIDFPGSDLTVPYEDLTLDLKPYEGKNVWILGRGNSAYEVAQHIMGVTNFIAMMA</sequence>
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    <accession>A0A1W2W711</accession>
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        <submittedName>
            <fullName evidence="7">Translation initiation factor IF-2-like</fullName>
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        <name evidence="7" type="primary">LOC100186360</name>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Phlebobranchia</taxon>
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            <taxon>Ciona</taxon>
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    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="7">wild type</strain>
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    <reference evidence="7" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="7" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="ENSCINP00000002777.3"/>
        <property type="gene ID" value="ENSCING00000010542.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100186360"/>
    <dbReference type="KEGG" id="cin:100186360"/>
    <dbReference type="HOGENOM" id="CLU_643968_0_0_1"/>
    <dbReference type="InParanoid" id="F6YS75"/>
    <dbReference type="OMA" id="ADYNEND"/>
    <dbReference type="OrthoDB" id="2020426at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="P:negative regulation of organ growth"/>
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        <property type="project" value="GO_Central"/>
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        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
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    <dbReference type="GO" id="GO:0035330">
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        <property type="project" value="GO_Central"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="PROTEIN KIBRA"/>
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    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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    <feature type="compositionally biased region" description="Low complexity" evidence="5">
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    </evidence>
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        <source>
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<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
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    <reference evidence="6" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">wild type</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="ENSCING00000006063.3"/>
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</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
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    <reference evidence="17" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
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                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
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                <person name="Nomoto H."/>
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                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <location evidence="2">Kinetochore</location>
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            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
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            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Spindle pole</location>
        </subcellularLocation>
    </comment>
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        <text evidence="13">Belongs to the TOG/XMAP215 family.</text>
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                <person name="Goodstein D.M."/>
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                <person name="Wang H.G."/>
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                <person name="Azumi K."/>
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                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
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                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
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                <person name="Rigoutsos I."/>
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                <person name="Tassy O."/>
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                <person name="Yagi K."/>
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                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
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        <source>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
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                <person name="Putnam N."/>
                <person name="Rash S."/>
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                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
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                <person name="Inaba K."/>
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                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
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                <person name="Miura S."/>
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                <person name="Nishizaka S."/>
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                <person name="Oishi K."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Wang H.G."/>
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                <person name="Makabe K.W."/>
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                <person name="Mochizuki Y."/>
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                <person name="Rigoutsos I."/>
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                <person name="Sasaki A."/>
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                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
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                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic ribosomal protein eS10 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002126653.1">
        <property type="nucleotide sequence ID" value="XM_002126617.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F7BK44"/>
    <dbReference type="FunCoup" id="F7BK44">
        <property type="interactions" value="561"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000012762"/>
    <dbReference type="Ensembl" id="ENSCINT00000012762.3">
        <property type="protein sequence ID" value="ENSCINP00000012762.3"/>
        <property type="gene ID" value="ENSCING00000006180.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100175151"/>
    <dbReference type="KEGG" id="cin:100175151"/>
    <dbReference type="GeneTree" id="ENSGT00940000166022"/>
    <dbReference type="HOGENOM" id="CLU_089349_3_1_1"/>
    <dbReference type="InParanoid" id="F7BK44"/>
    <dbReference type="OMA" id="YRRRDQE"/>
    <dbReference type="OrthoDB" id="5211809at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000025">
        <property type="entry name" value="40S ribosomal protein S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005326">
        <property type="entry name" value="Plectin_eS10_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037447">
        <property type="entry name" value="Ribosomal_eS10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12146">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12146:SF0">
        <property type="entry name" value="RIBOSOMAL PROTEIN S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03501">
        <property type="entry name" value="S10_plectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Plectin/eS10 N-terminal" evidence="7">
        <location>
            <begin position="3"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="88"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="97"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="137"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000012762.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="159" mass="18273" checksum="D0A2EA8474897F74" modified="2012-04-18" version="2">MLMPKKHRVLIYEHLFKEGVLVAIKDPFLDKHPELESVPNLHVMKACQSLKSRGYVRENFTWRHYYWILTNEGIQYLRDFLHLPPEIVPSTLKRQPRPTDTRMQRQKVESRPAYQSDRESYRHAPQGAGDKKAEAGAGTGGFDFRGAGYGRGRGAAPPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>F7BNX7</accession>
    <accession>A0A1W2WB58</accession>
    <name>F7BNX7_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">TRAF3-interacting protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC100184905</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Cilium axoneme</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium basal body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the TRAF3IP1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002128169.1">
        <property type="nucleotide sequence ID" value="XM_002128133.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F7BNX7"/>
    <dbReference type="FunCoup" id="F7BNX7">
        <property type="interactions" value="19"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000008934"/>
    <dbReference type="Ensembl" id="ENSCINT00000008934.4">
        <property type="protein sequence ID" value="ENSCINP00000008934.4"/>
        <property type="gene ID" value="ENSCING00000004318.4"/>
    </dbReference>
    <dbReference type="GeneID" id="100184905"/>
    <dbReference type="KEGG" id="cin:100184905"/>
    <dbReference type="GeneTree" id="ENSGT00720000108822"/>
    <dbReference type="HOGENOM" id="CLU_023216_0_0_1"/>
    <dbReference type="InParanoid" id="F7BNX7"/>
    <dbReference type="OMA" id="FRFLMDV"/>
    <dbReference type="OrthoDB" id="10258914at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030992">
        <property type="term" value="C:intraciliary transport particle B"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042073">
        <property type="term" value="P:intraciliary transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070507">
        <property type="term" value="P:regulation of microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.50:FF:000001">
        <property type="entry name" value="TRAF3-interacting protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.50">
        <property type="entry name" value="Microtubule-binding protein MIP-T3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018799">
        <property type="entry name" value="TRAF3IP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041476">
        <property type="entry name" value="TRAF3IP1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040468">
        <property type="entry name" value="TRAF3IP1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042576">
        <property type="entry name" value="TRAF3IP1_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31363">
        <property type="entry name" value="TRAF3-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31363:SF0">
        <property type="entry name" value="TRAF3-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10243">
        <property type="entry name" value="MIP-T3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17749">
        <property type="entry name" value="MIP-T3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="5 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TRAF3-interacting protein 1 N-terminal" evidence="12">
        <location>
            <begin position="6"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="TRAF3-interacting protein 1 C-terminal" evidence="13">
        <location>
            <begin position="388"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="123"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="408"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="465"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="138"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="170"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="266"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="305"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00043971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00070492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF10243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF17749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000008934.4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="550" mass="62051" checksum="199B001DE405602E" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>H2XKH1</accession>
    <name>H2XKH1_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FH2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the formin homology family. Cappuccino subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="H2XKH1"/>
    <dbReference type="STRING" id="7719.ENSCINP00000030153"/>
    <dbReference type="Ensembl" id="ENSCINT00000032567.1">
        <property type="protein sequence ID" value="ENSCINP00000030153.1"/>
        <property type="gene ID" value="ENSCING00000020464.1"/>
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    <dbReference type="GeneTree" id="ENSGT00940000161899"/>
    <dbReference type="HOGENOM" id="CLU_010208_2_0_1"/>
    <dbReference type="InParanoid" id="H2XKH1"/>
    <dbReference type="OMA" id="PERTHCI"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
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    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2220">
        <property type="entry name" value="Formin, FH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015425">
        <property type="entry name" value="FH2_Formin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042201">
        <property type="entry name" value="FH2_Formin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45920">
        <property type="entry name" value="FORMIN HOMOLOGY 2 DOMAIN CONTAINING, ISOFORM I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45920:SF7">
        <property type="entry name" value="FORMIN-G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02181">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00498">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51444">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FH2" evidence="2">
        <location>
            <begin position="1"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000030153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="302" mass="34544" checksum="C2952BE609291328" modified="2012-04-18" version="1">MKPLYWIRLNQSHSNQTIEQKKTLWENVEVVKLDEVDLVNLFAKTSSTKQKKPLNTTIGQKKKKKEKFGKVLDLKRSQAVGIFISSLHIDVDDIQNAILTLDTSIVDVEIMEAIWEIRPQLGEMEKIEHFVGTQKKVDEDQRLSLDRPEEFLYKLWQIPDLSHRLFCITFMSRFDQDVSHVTQTIALINDVCKTLRGDVVKKLLSIILSVGNYLNGGNVSRGQARGFDLEILGKLKDVKSNVGGVTLLSYIVSLYIRHFKQDNDLETWKAPVPDTLSLMRASQVKYEDICGEITKLKTKLNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>H2XL02</accession>
    <name>H2XL02_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Serine aminopeptidase S33 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01002466">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XL02"/>
    <dbReference type="STRING" id="7719.ENSCINP00000030334"/>
    <dbReference type="Ensembl" id="ENSCINT00000032955.1">
        <property type="protein sequence ID" value="ENSCINP00000030334.1"/>
        <property type="gene ID" value="ENSCING00000020115.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000011364"/>
    <dbReference type="HOGENOM" id="CLU_1964461_0_0_1"/>
    <dbReference type="InParanoid" id="H2XL02"/>
    <dbReference type="OMA" id="TNICERN"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051044">
        <property type="entry name" value="MAG_DAG_Lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11614">
        <property type="entry name" value="PHOSPHOLIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine aminopeptidase S33" evidence="1">
        <location>
            <begin position="45"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000030334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="128" mass="14426" checksum="F33756FB988B9F60" modified="2012-04-18" version="1">MSDSNNKVVKTFSGKLFSDMSHFVNADGLHIFTNCWEPKGDVNFLVCMLHGFGGHCIRFNELASYFTEIGGLVFSHDHIGHGESEGSRTTVDDYNKLIRDTYQHVDIMVEKYPGKPVYIFGQSMVCIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>H2XL66</accession>
    <name>H2XL66_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Tudor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="H2XL66"/>
    <dbReference type="STRING" id="7719.ENSCINP00000030398"/>
    <dbReference type="Ensembl" id="ENSCINT00000033328.1">
        <property type="protein sequence ID" value="ENSCINP00000030398.1"/>
        <property type="gene ID" value="ENSCING00000020439.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000001360"/>
    <dbReference type="HOGENOM" id="CLU_013593_1_0_1"/>
    <dbReference type="InParanoid" id="H2XL66"/>
    <dbReference type="OMA" id="QFYIHIC"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
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                <person name="De Tomaso A."/>
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                <person name="Hastings K.E."/>
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                <person name="Ogasawara M."/>
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                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
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                <person name="Yamada L."/>
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                <person name="Wiley G.B."/>
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                <person name="Roe B.A."/>
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                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
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                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
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                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
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                <person name="Suzuki M.M."/>
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                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
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                <person name="Wiley G.B."/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
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                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
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                <person name="Oishi K."/>
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                <person name="Suzuki M.M."/>
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                <person name="Hyatt P.D."/>
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                <person name="Detter C."/>
                <person name="Doggett N."/>
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                <person name="Richardson P."/>
                <person name="Lucas S."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="5">wild type</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
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                <person name="Chapman J."/>
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                <person name="De Tomaso A."/>
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                <person name="Di Gregorio A."/>
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                <person name="Terry A."/>
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                <person name="Wang H.G."/>
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    <reference evidence="6" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
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                <person name="Wiley G.B."/>
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                <person name="Lemaire P."/>
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            <strain evidence="2">wild type</strain>
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        <reaction evidence="8 9">
            <text>a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in mRNA + S-adenosyl-L-methionine = a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-(2'-O-methyl-ribonucleoside) in mRNA + S-adenosyl-L-homocysteine + H(+)</text>
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</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>H2XTB5</accession>
    <name>H2XTB5_CIOIN</name>
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        <submittedName>
            <fullName evidence="9">T-box-containing protein 2-like</fullName>
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        <name evidence="9" type="primary">LOC100185942</name>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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            <taxon>Tunicata</taxon>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
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                <person name="Nishizaka S."/>
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                <person name="Ohta F."/>
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                <person name="Rigoutsos I."/>
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                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        </subcellularLocation>
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        <property type="project" value="InterPro"/>
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    <sequence length="201" mass="23102" checksum="03755702FE3AAEF6" modified="2012-04-18" version="1">MATDVRGSTFEPKVHLEDWKLWEQFSAVQTEMIVTKTGRRMFPGYRVKMSGMDPNAQYCVLMDISSVDENRYKFQHGEWVVAGRGEPQVPQRFYLHPNSPCSGQQWMKEIISFHKVKLTNSCGNSADGKFLIHSMHRYQPRIHIVRTDDVNTLHLQPMSTFAFPQTAFVTVTAYQNGQITKLKIANNPFARGFREDGAKTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>H2XU43</accession>
    <name>H2XU43_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
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        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
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                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
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                <person name="Hayashi H."/>
                <person name="Hino K."/>
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                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="12" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="12">wild type</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
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                <person name="Nishizaka S."/>
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                <person name="Oishi K."/>
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                <person name="Yagi K."/>
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                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
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                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
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                <person name="Nomoto H."/>
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                <person name="Rigoutsos I."/>
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                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
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                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
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                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
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                <person name="Lindquist E."/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
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                <person name="Di Gregorio A."/>
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                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
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                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
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    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <location>
            <begin position="1"/>
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    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
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        </location>
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        <location>
            <begin position="21"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    <evidence type="ECO:0000313" key="2">
        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    <sequence length="61" mass="6166" checksum="88789BA114D3E0E3" modified="2012-04-18" version="1">MPPIPPMPPIPPPAGIGGSSFGSSVTTHSAVVSNEATPEASIRACFTTFVGSRIPASSMLR</sequence>
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    <accession>H2XVV4</accession>
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            <fullName evidence="1">Uncharacterized protein</fullName>
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        <name type="common">Transparent sea squirt</name>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
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                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    <dbReference type="Ensembl" id="ENSCINT00000033144.1">
        <property type="protein sequence ID" value="ENSCINP00000033788.1"/>
        <property type="gene ID" value="ENSCING00000020068.1"/>
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    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000033788.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
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    <sequence length="53" mass="5498" checksum="F20D0341DDEA4CD4" modified="2012-04-18" version="1">SINDINAAAPILFVSIFLTHCLVCPITVNNGGCLGKFCDSSIALFKAVQTGSL</sequence>
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    <accession>H2XVW4</accession>
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            <fullName evidence="2">alanine--tRNA ligase</fullName>
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        <name type="scientific">Ciona intestinalis</name>
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        <name type="synonym">Ascidia intestinalis</name>
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    <reference evidence="15" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
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                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
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                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>tRNA(Ala) + L-alanine + ATP = L-alanyl-tRNA(Ala) + AMP + diphosphate</text>
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            <dbReference type="Rhea" id="RHEA-COMP:9657"/>
            <dbReference type="Rhea" id="RHEA-COMP:9923"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78497"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.7"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
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    <dbReference type="AlphaFoldDB" id="H2XVW4"/>
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        <property type="protein sequence ID" value="ENSCINP00000033798.1"/>
        <property type="gene ID" value="ENSCING00000021413.1"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="term" value="F:alanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
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        <property type="project" value="GO_Central"/>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:alanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Alanine--tRNA ligase, cytoplasmic"/>
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        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
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    <dbReference type="InterPro" id="IPR002318">
        <property type="entry name" value="Ala-tRNA-lgiase_IIc"/>
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    <dbReference type="InterPro" id="IPR018162">
        <property type="entry name" value="Ala-tRNA-ligase_IIc_anticod-bd"/>
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        <property type="entry name" value="Ala-tRNA-synth_IIc_core"/>
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        <property type="entry name" value="Ala-tRNA-synth_IIc_N"/>
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    <dbReference type="InterPro" id="IPR050058">
        <property type="entry name" value="Ala-tRNA_ligase"/>
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        <property type="entry name" value="alaS"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR11777:SF9">
        <property type="entry name" value="ALANINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR11777">
        <property type="entry name" value="ALANYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF01411">
        <property type="entry name" value="tRNA-synt_2c"/>
        <property type="match status" value="1"/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">wild type</strain>
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    <reference evidence="10" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <location evidence="1">Nucleus</location>
        </subcellularLocation>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="De Tomaso A."/>
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                <person name="Di Gregorio A."/>
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                <person name="Mount S."/>
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                <person name="Tassy O."/>
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                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
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                <person name="Doggett N."/>
                <person name="Glavina T."/>
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                <person name="Richardson P."/>
                <person name="Lucas S."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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    <dbReference type="InParanoid" id="H2XWR0"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000034094.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    </evidence>
    <sequence length="47" mass="5240" checksum="EED22157F8493D1E" modified="2012-04-18" version="1">MSFEIISGYSYWYATMSDNNVAVFPVPDGISRTPCPFASNVRFNSSI</sequence>
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<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>H2XX75</accession>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
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    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <source>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Putnam N."/>
                <person name="Rash S."/>
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                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
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                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
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                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
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                <person name="Nishizaka S."/>
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                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
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                <person name="Sasaki A."/>
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                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
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                <person name="Satoh N."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="H2Y368"/>
    <dbReference type="Ensembl" id="ENSCINT00000031198.1">
        <property type="protein sequence ID" value="ENSCINP00000036353.1"/>
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        <source>
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    <sequence length="21" mass="2617" checksum="2B6EC30443A7E09A" modified="2012-04-18" version="1">LMITVIWKVLLNIERYKNNKK</sequence>
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    <reference evidence="15" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
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                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
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                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
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                <person name="Hyatt P.D."/>
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                <person name="Doggett N."/>
                <person name="Glavina T."/>
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                <person name="Richardson P."/>
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                <person name="Levine M."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">wild type</strain>
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    <reference evidence="14" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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    <comment type="function">
        <text evidence="1 11">The glycine cleavage system catalyzes the degradation of glycine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 11">
            <text>N(6)-[(R)-S(8)-aminomethyldihydrolipoyl]-L-lysyl-[protein] + (6S)-5,6,7,8-tetrahydrofolate = N(6)-[(R)-dihydrolipoyl]-L-lysyl-[protein] + (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:16945"/>
            <dbReference type="Rhea" id="RHEA-COMP:10475"/>
            <dbReference type="Rhea" id="RHEA-COMP:10492"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:83100"/>
            <dbReference type="ChEBI" id="CHEBI:83143"/>
            <dbReference type="EC" id="2.1.2.10"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4 11">The glycine cleavage system is composed of four proteins: P, T, L and H.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the GcvT family.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.10" evidence="11"/>
    <dbReference type="EMBL" id="EAAA01002642">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="interactions" value="10"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000036541"/>
    <dbReference type="Ensembl" id="ENSCINT00000035941.1">
        <property type="protein sequence ID" value="ENSCINP00000036541.1"/>
        <property type="gene ID" value="ENSCING00000023450.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157524"/>
    <dbReference type="HOGENOM" id="CLU_007884_10_0_1"/>
    <dbReference type="InParanoid" id="H2Y3Q6"/>
    <dbReference type="OMA" id="MPVQYPA"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005960">
        <property type="term" value="C:glycine cleavage complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004047">
        <property type="term" value="F:aminomethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006546">
        <property type="term" value="P:glycine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.110:FF:000002">
        <property type="entry name" value="Aminomethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.120:FF:000014">
        <property type="entry name" value="Aminomethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1400:FF:000001">
        <property type="entry name" value="Aminomethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1250.10:FF:000002">
        <property type="entry name" value="Aminomethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.110">
        <property type="entry name" value="Aminomethyltransferase beta-barrel domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1400">
        <property type="entry name" value="Aminomethyltransferase beta-barrel domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1250.10">
        <property type="entry name" value="Aminomethyltransferase fragment"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.120">
        <property type="entry name" value="Probable tRNA modification gtpase trme, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006223">
        <property type="entry name" value="GcvT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028896">
        <property type="entry name" value="GcvT/YgfZ/DmdA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029043">
        <property type="entry name" value="GcvT/YgfZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013977">
        <property type="entry name" value="GcvT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006222">
        <property type="entry name" value="GCVT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027266">
        <property type="entry name" value="TrmE/GcvT-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00528">
        <property type="entry name" value="gcvT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001567">
        <property type="entry name" value="PRK00389.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43757">
        <property type="entry name" value="AMINOMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43757:SF16">
        <property type="entry name" value="AMINOMETHYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01571">
        <property type="entry name" value="GCV_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08669">
        <property type="entry name" value="GCV_T_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006487">
        <property type="entry name" value="GcvT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101790">
        <property type="entry name" value="Aminomethyltransferase beta-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103025">
        <property type="entry name" value="Folate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="8 11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 11" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="GCVT N-terminal" evidence="12">
        <location>
            <begin position="6"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="Aminomethyltransferase C-terminal" evidence="13">
        <location>
            <begin position="286"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR006487-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000036541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="371" mass="40363" checksum="1F92396EF7D07AF5" modified="2012-04-18" version="1">IKTSVLHDFHVKNNAKMVPFAGWTMPIQYKSLGIIDSHHHTRNKVSLFDVSHMLQFKVYGKDKESFIESMTVCDVKGLPENGGSLTVFTNAEGGIMDDAIINQTQQDYLYCVSNAGCSDKISTCLRENLIDFTAKGGEVVLELLDCGLLAVQGPKMAEVLQTGTDTDLSKLYFMQNVEANLFGVDCRITRCGYTGEDGVEISVAKNRAVELAENICSHEDVELAGLGARDSLRLEAGLCLYGNDIDEMTTPVEAGLTWCIGKRRRKEKNFPGAEKIVAQIKSKPSKRRSGLIVSSAIARNGAIVQDGNGNEIGSVTSGCPSPTLSANIAMAYLPLPLSKVGTEVNVLVRKRVVSAKVTKMPFVPANYFMNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-03-06" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>L7N0T9</accession>
    <name>L7N0T9_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Sorting nexin C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="L7N0T9"/>
    <dbReference type="Ensembl" id="ENSCINT00000019940.3">
        <property type="protein sequence ID" value="ENSCINP00000019940.3"/>
        <property type="gene ID" value="ENSCING00000009820.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182856"/>
    <dbReference type="HOGENOM" id="CLU_1049543_0_0_1"/>
    <dbReference type="InParanoid" id="L7N0T9"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013937">
        <property type="entry name" value="Sorting_nexin_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22775">
        <property type="entry name" value="SORTING NEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22775:SF3">
        <property type="entry name" value="SORTING NEXIN-13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08628">
        <property type="entry name" value="Nexin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sorting nexin C-terminal" evidence="2">
        <location>
            <begin position="115"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000019940.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="265" mass="30359" checksum="587248B880CD76F8" modified="2013-03-06" version="1">MPILNESKPSCSSHRSAHTKHKGPVTRFKNIMLGKTLDSDKYEDVVRDYSPFTGPRNSNLVTFGRKWRSPENLLTNRTRFCASSMGGRRTQGDGCDNVISLEQHGNGLLGDCILAGQVFRVAKIIWNIPLGKHSLSVVCVARFVQQYMEIKIQQLLQKDKWMHYLKTFQNSLWPSGVWGETDTKENEDPKENYDKAQLVLEDTFPDVLRFLVGDAEYKHGVSSLLESFQHPVINRHLIYHLLDSSLQLLFPDFQNILAKRRNISI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-06-07" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>P61802</accession>
    <name>APTX_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName>Aprataxin</fullName>
            <ecNumber evidence="3">3.6.1.71</ecNumber>
            <ecNumber evidence="2">3.6.1.72</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Forkhead-associated domain histidine triad-like protein</fullName>
            <shortName>FHA-HIT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">APTX</name>
    </gene>
    <organism>
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2002-12" db="EMBL/GenBank/DDBJ databases">
            <title>Identification of human FHA-HIT gene homolog in sea squirt.</title>
            <authorList>
                <person name="Chen Y."/>
                <person name="Huang C.-H."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [MRNA]</scope>
    </reference>
    <comment type="function">
        <text evidence="2 3">DNA-binding protein involved in single-strand DNA break repair, double-strand DNA break repair and base excision repair. Resolves abortive DNA ligation intermediates formed either at base excision sites, or when DNA ligases attempt to repair non-ligatable breaks induced by reactive oxygen species. Catalyzes the release of adenylate groups covalently linked to 5'-phosphate termini, resulting in the production of 5'-phosphate termini that can be efficiently rejoined. Also able to hydrolyze adenosine 5'-monophosphoramidate (AMP-NH(2)) and diadenosine tetraphosphate (AppppA), but with lower catalytic activity (By similarity). Likewise, catalyzes the release of 3'-linked guanosine (DNAppG) and inosine (DNAppI) from DNA, but has higher specific activity with 5'-linked adenosine (AppDNA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>a 5'-end adenosine-5'-diphospho-5'-2'-deoxyribonucleoside-DNA + H2O = a 5'-end 5'-phospho-2'-deoxyribonucleoside-DNA + AMP + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:52128"/>
            <dbReference type="Rhea" id="RHEA-COMP:13180"/>
            <dbReference type="Rhea" id="RHEA-COMP:13181"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:136412"/>
            <dbReference type="ChEBI" id="CHEBI:136413"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="3.6.1.71"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>a 5'-end adenosine-5'-diphospho-5'-ribonucleoside-2'-deoxyribonucleotide-DNA + H2O = a 5'-end 5'-phospho-ribonucleoside-2'-deoxyribonucleotide-DNA + AMP + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:52132"/>
            <dbReference type="Rhea" id="RHEA-COMP:13182"/>
            <dbReference type="Rhea" id="RHEA-COMP:13183"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:136414"/>
            <dbReference type="ChEBI" id="CHEBI:136415"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="3.6.1.71"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a 3'-end 2'-deoxyribonucleotide-3'-diphospho-5'-guanosine-DNA + H2O = a 3'-end 2'-deoxyribonucleotide 3'-phosphate-DNA + GMP + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:52140"/>
            <dbReference type="Rhea" id="RHEA-COMP:13186"/>
            <dbReference type="Rhea" id="RHEA-COMP:13187"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:136419"/>
            <dbReference type="ChEBI" id="CHEBI:136420"/>
            <dbReference type="EC" id="3.6.1.72"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">Nucleoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="3">The histidine triad, also called HIT motif, forms part of the binding loop for the alpha-phosphate of purine mononucleotide.</text>
    </comment>
    <comment type="domain">
        <text evidence="1">The HIT domain is required for enzymatic activity.</text>
    </comment>
    <comment type="domain">
        <text evidence="1">The C2H2-type zinc finger mediates DNA-binding.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.71" evidence="3"/>
    <dbReference type="EC" id="3.6.1.72" evidence="2"/>
    <dbReference type="EMBL" id="AY208848">
        <property type="protein sequence ID" value="AAP86338.1"/>
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    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
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    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <keyword id="KW-0863">Zinc-finger</keyword>
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            <begin position="1"/>
            <end position="380"/>
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    </feature>
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</entry>
<entry dataset="TrEMBL" created="2017-06-07" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1W3JQT9</accession>
    <accession>F7BFN9</accession>
    <name>A0A1W3JQT9_CIOIN</name>
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        <submittedName>
            <fullName evidence="8">Coadhesin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100185064</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">wild type</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01000437">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002127606.1">
        <property type="nucleotide sequence ID" value="XM_002127570.4"/>
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    <dbReference type="AlphaFoldDB" id="A0A1W3JQT9"/>
    <dbReference type="Ensembl" id="ENSCINT00000027216.2">
        <property type="protein sequence ID" value="ENSCINP00000026970.2"/>
        <property type="gene ID" value="ENSCING00000015081.2"/>
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    <dbReference type="GeneID" id="100185064"/>
    <dbReference type="KEGG" id="cin:100185064"/>
    <dbReference type="eggNOG" id="KOG4475">
        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="GeneTree" id="ENSGT00940000166853"/>
    <dbReference type="InParanoid" id="A0A1W3JQT9"/>
    <dbReference type="OMA" id="GHACICH"/>
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    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 10"/>
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        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="2"/>
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    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="4"/>
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    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
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    <dbReference type="InterPro" id="IPR051418">
        <property type="entry name" value="Spondin/Thrombospondin_T1"/>
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        <property type="entry name" value="TSP1_rpt"/>
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        <property type="entry name" value="TSP1_rpt_sf"/>
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    <dbReference type="InterPro" id="IPR044004">
        <property type="entry name" value="TSP1_spondin_dom"/>
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        <property type="entry name" value="SPONDIN"/>
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        <property type="entry name" value="SPONDIN-LIKE TSP1 DOMAIN-CONTAINING PROTEIN"/>
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        <property type="entry name" value="TSP1"/>
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    <keyword evidence="4" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
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    <feature type="chain" id="PRO_5014069088" evidence="6">
        <location>
            <begin position="22"/>
            <end position="487"/>
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            <begin position="380"/>
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            <begin position="451"/>
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            <begin position="477"/>
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            <begin position="409"/>
            <end position="418"/>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000026970.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="487" mass="51587" checksum="CF3833873120EA29" modified="2017-06-07" version="1">MKGKGFLILFVLLFLVIQGEAWRRRRRRRRACTAVNCAWRLGTCSTTCGPGTKTYVKTRVNSCGGSCVQPAAVACNLRACPINCVTSAWSSWGTCSKTCGAGGVEIRTRTVTTAAQHGGTACPSLTESKACNQVCMNNGTFTGGRCVCVGSYTGTCCQTPPPVHCAMSAWSTWGTCSKTCGAGGVAVRTRSVVTHSQHGGRNCTGALRETKSCNEICLNSGNFTSNVVNNVTHSSCKCHAGYAGACCQRKVPVHCVTSAWSAWSNCSKTCGGGESFRTRSIVTPHKHNGTVCPALRETKACNMICYNGGNYSTNGCVCVGGHNGSCCEIKPSPVHCVLSTWSSWSNCSKTCGAGGVKTRTKSVVTEALYGGRNCSGNRSESKPCNHICYNGGNYSLIQTPYKIGHACICHGGYTGSCCQVPPGINIDLTINIYETIYVIWEPYNPTHHRSHSCGGMGAGGEGEEGEGEGGGMRWDEDKEETELESLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-07" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>C4B8X5</accession>
    <accession>A0A1W3JR02</accession>
    <name>C4B8X5_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Radial spoke head 1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">Ci-MORN40</name>
        <name evidence="13" type="synonym">ci-morn40</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">wild type</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="journal article" date="2009" name="FEBS Lett." volume="583" first="2201" last="2207">
            <title>Proteomic characterization of sperm radial spokes identifies a novel spoke protein with an ubiquitin domain.</title>
            <authorList>
                <person name="Satouh Y."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="19527718"/>
            <dbReference type="DOI" id="10.1016/j.febslet.2009.06.016"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Testis</tissue>
        </source>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Functions as part of axonemal radial spoke complexes that play an important part in the motility of sperm and cilia.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Flagellum axoneme</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="EAAA01000597">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AB501299">
        <property type="protein sequence ID" value="BAH59284.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001155276.1">
        <property type="nucleotide sequence ID" value="NM_001161804.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009859932.1">
        <property type="nucleotide sequence ID" value="XM_009861630.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C4B8X5"/>
    <dbReference type="IntAct" id="C4B8X5">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="MINT" id="C4B8X5"/>
    <dbReference type="STRING" id="7719.ENSCINP00000012862"/>
    <dbReference type="Ensembl" id="ENSCINT00000012862.3">
        <property type="protein sequence ID" value="ENSCINP00000012862.3"/>
        <property type="gene ID" value="ENSCING00000006238.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100177304"/>
    <dbReference type="KEGG" id="cin:100177304"/>
    <dbReference type="CTD" id="100177304"/>
    <dbReference type="GeneTree" id="ENSGT00940000157240"/>
    <dbReference type="HOGENOM" id="CLU_032017_2_0_1"/>
    <dbReference type="OMA" id="IYEGAWF"/>
    <dbReference type="OrthoDB" id="423343at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035082">
        <property type="term" value="P:axoneme assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007286">
        <property type="term" value="P:spermatid development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.110.10:FF:000002">
        <property type="entry name" value="Phosphatidylinositol 4-phosphate 5-kinase 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.110.10:FF:000010">
        <property type="entry name" value="Radial spoke head 1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.110.10">
        <property type="entry name" value="Histone H3 K4-specific methyltransferase SET7/9 N-terminal domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215:SF14">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82185">
        <property type="entry name" value="Histone H3 K4-specific methyltransferase SET7/9 N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="8" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5" id="KW-0969">Cilium</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0282">Flagellum</keyword>
    <keyword evidence="7" id="KW-0469">Meiosis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="221"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="21"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="222"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="272"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00056649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00073772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BAH59284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000012862.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="300" mass="33224" checksum="228638035378DDEB" modified="2009-07-07" version="1">MSDLGSDEFEDEAGPYLGEYEGDRNEEGERHGYGKATLPNGDTYEGQYDKGKRHGQGTYRFKNNARYIGEYLRNKKHGQGTFIYPDGSKYEGSWVDDQRHGNGKYFYVNADSYEGEWLKHERHGQGVYIYSDTGSKYVGTWVSGKCEGAGEFVHANHRFQGNWTDGSMQGPGKYIFDIGCEQHGEYIPVEQAQGDGEEEESVTVTIPKWKAGKITAITLYEPEEKAANDEVENEKVPTPEEEVKASEEAPQSPAPADPEPEETQPPAAEEAPAAEEAEEEATEDAEPAEAEDAAPAEDDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-07" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>C4B8X6</accession>
    <accession>A0A1W2W0W6</accession>
    <accession>F6W9N0</accession>
    <name>C4B8X6_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Radial spoke head protein 9 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">Ci-RSP9</name>
        <name evidence="12" type="synonym">ci-rsp9</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2009" name="FEBS Lett." volume="583" first="2201" last="2207">
            <title>Proteomic characterization of sperm radial spokes identifies a novel spoke protein with an ubiquitin domain.</title>
            <authorList>
                <person name="Satouh Y."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="19527718"/>
            <dbReference type="DOI" id="10.1016/j.febslet.2009.06.016"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Testis</tissue>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell projection</location>
            <location evidence="8">Kinocilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Flagellum axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the flagellar radial spoke RSP9 family.</text>
    </comment>
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        <property type="protein sequence ID" value="BAH59285.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001154962.1">
        <property type="nucleotide sequence ID" value="NM_001161490.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C4B8X6"/>
    <dbReference type="IntAct" id="C4B8X6">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="MINT" id="C4B8X6"/>
    <dbReference type="STRING" id="7719.ENSCINP00000026516"/>
    <dbReference type="Ensembl" id="ENSCINT00000026762.2">
        <property type="protein sequence ID" value="ENSCINP00000026516.2"/>
        <property type="gene ID" value="ENSCING00000014743.2"/>
    </dbReference>
    <dbReference type="GeneID" id="100179789"/>
    <dbReference type="KEGG" id="cin:100179789"/>
    <dbReference type="CTD" id="100179789"/>
    <dbReference type="GeneTree" id="ENSGT00390000018686"/>
    <dbReference type="HOGENOM" id="CLU_068343_1_0_1"/>
    <dbReference type="OMA" id="TFYHVPN"/>
    <dbReference type="OrthoDB" id="10258956at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060091">
        <property type="term" value="C:kinocilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001534">
        <property type="term" value="C:radial spoke"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035082">
        <property type="term" value="P:axoneme assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060294">
        <property type="term" value="P:cilium movement involved in cell motility"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044458">
        <property type="term" value="P:motile cilium assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006802">
        <property type="entry name" value="Radial_spoke"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055316">
        <property type="entry name" value="RSP9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22069">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOMAL PROTEIN S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22069:SF0">
        <property type="entry name" value="RADIAL SPOKE HEAD PROTEIN 9 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04712">
        <property type="entry name" value="Radial_spoke"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5" id="KW-0969">Cilium</keyword>
    <keyword evidence="3" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0282">Flagellum</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00041080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAH59285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000026516.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="276" mass="31054" checksum="3FEC3C84112ABF78" modified="2009-07-07" version="1">MDANGLHLNVDHVAGSGVILSPEQKAALQTSLIILKTHYKFNNVVLWGKILGIKDDYFIAQGSGKDELKDRKSLYSLNCIEWHLLPPATSEMKNKATMVRGRLMGDPSFEYEHTDVRRVRDGEEYTEEEVTIQIKEEDRVSAIVATIDEEASIVPRGAFIKTPLGQVYNNRSFEGLSVAECGKLSSFMHFREAQNFGNKSIKEKADLNPSIDFLDSIEDDIPKGSWSSQFERGSGLVCLRSLVWLGMTFYHVPNTTRFGCMYVGTGEKNFDIPFML</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>F6PIR1</accession>
    <name>F6PIR1_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">USP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01001505">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6PIR1"/>
    <dbReference type="STRING" id="7719.ENSCINP00000020217"/>
    <dbReference type="Ensembl" id="ENSCINT00000020217.3">
        <property type="protein sequence ID" value="ENSCINP00000020217.3"/>
        <property type="gene ID" value="ENSCING00000010098.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157267"/>
    <dbReference type="HOGENOM" id="CLU_073498_1_0_1"/>
    <dbReference type="InParanoid" id="F6PIR1"/>
    <dbReference type="OMA" id="HCIKRIT"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000377">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF842">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="USP" evidence="1">
        <location>
            <begin position="41"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000020217.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="151" mass="16964" checksum="478306760F43AC02" modified="2012-04-18" version="2">MFGNLFEDGSNMACRNAKSVLSINGKPIEQPPEERGEQSLIGIQNQGATCYLNSLIQTLFLTPEFREQLFDIGCEELGKSGPNKKDGKVRKIPLQLQLLFARLLLINQSSCKTEDLTDSFGWRNSEEMQQHDVQELSRILFDAIENSLVGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>F6PKH1</accession>
    <name>F6PKH1_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
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                <person name="Macmil S.L."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
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    <reference evidence="2" key="3">
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            <authorList>
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            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="7" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">wild type</strain>
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    <reference evidence="7" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Stress granule</location>
        </subcellularLocation>
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        <property type="project" value="GO_Central"/>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="entry name" value="NTF2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="RRM_G3BP"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Ras GTPase-activating protein-binding protein"/>
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            <end position="134"/>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
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    <name>F6PNI9_CIOIN</name>
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        </lineage>
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    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">wild type</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rho family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002956">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6PNI9"/>
    <dbReference type="FunCoup" id="F6PNI9">
        <property type="interactions" value="4"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000004757"/>
    <dbReference type="Ensembl" id="ENSCINT00000004757.3">
        <property type="protein sequence ID" value="ENSCINP00000004757.3"/>
        <property type="gene ID" value="ENSCING00000002339.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155205"/>
    <dbReference type="HOGENOM" id="CLU_041217_21_3_1"/>
    <dbReference type="InParanoid" id="F6PNI9"/>
    <dbReference type="OMA" id="VIFRPAQ"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060326">
        <property type="term" value="P:cell chemotaxis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030031">
        <property type="term" value="P:cell projection assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0030865">
        <property type="term" value="P:cortical cytoskeleton organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007163">
        <property type="term" value="P:establishment or maintenance of cell polarity"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008045">
        <property type="term" value="P:motor neuron axon guidance"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016601">
        <property type="term" value="P:Rac protein signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032956">
        <property type="term" value="P:regulation of actin cytoskeleton organization"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902622">
        <property type="term" value="P:regulation of neutrophil migration"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000088">
        <property type="entry name" value="Ras-related C3 botulinum toxin substrate 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0488">Methylation</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0636">Prenylation</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000004757.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="194" mass="21217" checksum="2DEC803EEAEF480B" modified="2012-04-18" version="2">QAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDGRMINLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPTSLQNVGAKWFPEISHHAPGTAIVLVGTKCDLREDPETLKALAEKNLTPVTTSQGLQMAKELNAVKFLECSALTQKGVKAVFEEAIRAVISPTKQAAKKKKQKSCRLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>F6PRU7</accession>
    <accession>A0A1W5BEH8</accession>
    <name>F6PRU7_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">4-hydroxyphenylpyruvate dioxygenase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">4-hydroxyphenylpyruvic acid oxidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100181413</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">wild type</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the conversion of 4-hydroxyphenylpyruvic acid to homogentisic acid, one of the steps in tyrosine catabolism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>3-(4-hydroxyphenyl)pyruvate + O2 = homogentisate + CO2</text>
            <dbReference type="Rhea" id="RHEA:16189"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16169"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:36242"/>
            <dbReference type="EC" id="1.13.11.27"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:16190"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the 4HPPD family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002669">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018668547.2">
        <property type="nucleotide sequence ID" value="XM_018813002.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6PRU7"/>
    <dbReference type="STRING" id="7719.ENSCINP00000013076"/>
    <dbReference type="Ensembl" id="ENSCINT00000013076.3">
        <property type="protein sequence ID" value="ENSCINP00000013076.3"/>
        <property type="gene ID" value="ENSCING00000006343.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100181413"/>
    <dbReference type="KEGG" id="cin:100181413"/>
    <dbReference type="GeneTree" id="ENSGT00530000063474"/>
    <dbReference type="HOGENOM" id="CLU_034004_2_0_1"/>
    <dbReference type="InParanoid" id="F6PRU7"/>
    <dbReference type="OMA" id="PTLMRWF"/>
    <dbReference type="OrthoDB" id="414569at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003868">
        <property type="term" value="F:4-hydroxyphenylpyruvate dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009072">
        <property type="term" value="P:aromatic amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07250">
        <property type="entry name" value="HPPD_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="entry name" value="2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005956">
        <property type="entry name" value="4OHPhenylPyrv_dOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041735">
        <property type="entry name" value="4OHPhenylPyrv_dOase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004360">
        <property type="entry name" value="Glyas_Fos-R_dOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC_core"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11959">
        <property type="entry name" value="4-HYDROXYPHENYLPYRUVATE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11959:SF10">
        <property type="entry name" value="4-HYDROXYPHENYLPYRUVATE DIOXYGENASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00903">
        <property type="entry name" value="Glyoxalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="VOC" evidence="10">
        <location>
            <begin position="170"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000013076.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="414" mass="47255" checksum="4C22BF7E32690021" modified="2011-07-27" version="1">MYHSIHHIRYSVKNVEKFSSFLINQLNFKKYATSKASDCPLRSKTIVLRHADVIFIIDQWKEGAEPCCDNFFCHRNNNYPVNTACDVCLQTSDVEFILQKAKSASPKNSHKETDHIGDKDGIVSYAIIKSPVGNLQHTLIDLTKYGGAFLPGFEVNKSWDPESHPHFMTAVDHIALSLQLEHTDEVVKWYNHCLGFTRMIINTEEDFDAGFVVKEGIHGLRLKAVLGPQQLEEQKVKNKVPEVPKFVFCEALKQDKRSQIEHFLFMHQGEGIQHIAFSTNNIVDAISNFKASGLQCVLPPDDYYAEQFPQNQIENLDYTVETLKANSILLESNHGEAESGSDWCELNFGVSRLEDQVQHMNKTNWFIMQAFTTPVFEEKTLFFEIIQRFQTISGFGAGNIGALWRAVQKELFNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>F6PS78</accession>
    <name>F6PS78_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Peroxisomal 2,4-dienoyl-CoA reductase [(3E)-enoyl-CoA-producing]</fullName>
            <ecNumber evidence="9">1.3.1.124</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">2,4-dienoyl-CoA reductase 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">wild type</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>(2E,4Z,7Z,10Z,13Z,16Z,19Z)-docosaheptaenoyl-CoA + NADPH + H(+) = (3E,7Z,10Z,13Z,16Z,19Z)-docosahexaenoyl-CoA + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:44920"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:77559"/>
            <dbReference type="ChEBI" id="CHEBI:84791"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a (2E,4E)-dienoyl-CoA + NADPH + H(+) = a 4,5-saturated-(3E)-enoyl-CoA + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:45912"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:85101"/>
            <dbReference type="ChEBI" id="CHEBI:85493"/>
            <dbReference type="EC" id="1.3.1.124"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a (2E,4Z)-dienoyl-CoA + NADPH + H(+) = a 4,5-saturated-(3E)-enoyl-CoA + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:61892"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:85099"/>
            <dbReference type="ChEBI" id="CHEBI:85493"/>
            <dbReference type="EC" id="1.3.1.124"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Monomer, dimer and oligomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the short-chain dehydrogenases/reductases (SDR) family. 2,4-dienoyl-CoA reductase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.124" evidence="9"/>
    <dbReference type="EMBL" id="EAAA01001699">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6PS78"/>
    <dbReference type="FunCoup" id="F6PS78">
        <property type="interactions" value="60"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000025045"/>
    <dbReference type="Ensembl" id="ENSCINT00000025291.2">
        <property type="protein sequence ID" value="ENSCINP00000025045.2"/>
        <property type="gene ID" value="ENSCING00000013694.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153801"/>
    <dbReference type="HOGENOM" id="CLU_010194_1_2_1"/>
    <dbReference type="InParanoid" id="F6PS78"/>
    <dbReference type="OMA" id="ITPMGRP"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008670">
        <property type="term" value="F:2,4-dienoyl-CoA reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009062">
        <property type="term" value="P:fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd05369">
        <property type="entry name" value="TER_DECR_SDR_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000477">
        <property type="entry name" value="Peroxisomal 2,4-dienoyl-CoA reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045017">
        <property type="entry name" value="DECR2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43296">
        <property type="entry name" value="PEROXISOMAL 2,4-DIENOYL-COA REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43296:SF2">
        <property type="entry name" value="PEROXISOMAL 2,4-DIENOYL-COA REDUCTASE [(3E)-ENOYL-COA-PRODUCING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="3" id="KW-0521">NADP</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00026117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00026221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000025045.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="292" mass="31397" checksum="D42AD82AFCA93EEB" modified="2012-04-18" version="2">MATRNLNIEANGDDCLQDYNYVFKPDLLKDKVAFITGGGTGIGFRITEIFMRHGCKTAIASRKIERVKEAADKLTAATGVECFPLQMDVRNPAEVDQTVKNIVSHFGKLDILVNNAAGNFLCPAESLSANAFKTVMEIDTNGTFNVTKSVFDNYFKENGGSVVNITATLGYKGTVMQTHAGCAKAAIDTMTKHLAAEWGQYGVRINGIAPGPIGGTVGMAKLGGKSDKAEMMKKSIPLQRWGQKTEIADSVLYLVSPAASYVTGTVLVADGGSWLTSFNDPTMMHMLMKSSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>F6PZ24</accession>
    <accession>A0A1W2WFM1</accession>
    <name>F6PZ24_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized LOC100176919</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100176919</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">wild type</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum-Golgi intermediate compartment membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Golgi apparatus</location>
            <location evidence="3">cis-Golgi network membrane</location>
            <topology evidence="3">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Membrane</location>
            <topology evidence="9">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 9">Belongs to the EMP24/GP25L family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001210">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002130338.1">
        <property type="nucleotide sequence ID" value="XM_002130302.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6PZ24"/>
    <dbReference type="STRING" id="7719.ENSCINP00000011872"/>
    <dbReference type="Ensembl" id="ENSCINT00000011872.3">
        <property type="protein sequence ID" value="ENSCINP00000011872.3"/>
        <property type="gene ID" value="ENSCING00000005752.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100176919"/>
    <dbReference type="KEGG" id="cin:100176919"/>
    <dbReference type="GeneTree" id="ENSGT00940000166069"/>
    <dbReference type="HOGENOM" id="CLU_1085693_0_0_1"/>
    <dbReference type="InParanoid" id="F6PZ24"/>
    <dbReference type="OMA" id="NESETFM"/>
    <dbReference type="OrthoDB" id="10037706at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005793">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033116">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015720">
        <property type="entry name" value="Emp24-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009038">
        <property type="entry name" value="GOLD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036598">
        <property type="entry name" value="GOLD_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22811">
        <property type="entry name" value="TRANSMEMBRANE EMP24 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01105">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01190">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101576">
        <property type="entry name" value="Supernatant protein factor (SPF), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50866">
        <property type="entry name" value="GOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0732">Signal</keyword>
    <keyword evidence="5 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014089910" evidence="11">
        <location>
            <begin position="17"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="221"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="GOLD" evidence="12">
        <location>
            <begin position="62"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000011872.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="256" mass="28916" checksum="5F4987F2E3BF55A5" modified="2012-04-18" version="2">MQLLLLFLVFLQCACAQEGYTSEDMSQQLYGTKFANEFFRSLQRGSLLHEFGIKTYVAAGSTECYFQYCAAGAELVVGFEMATSGGVANKGLLSGYVSSPNGTRLLVSSEPKNEQQFEVECAEKGVYEFCLDNTGDHLFPKLAYFHIVVLNKQLFTTFHESVQNSNESETFMTTMYQVFDKVFHINKNWMVVKMVQHHKEAMKHRDIDTLVSNAWRVSSTAMATIMVTLFVGLAQVYSVRRMFNEKSGKGKTALRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>F6Q301</accession>
    <name>F6Q301_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ditrans,polycis-polyprenyl diphosphate synthase [(2E,6E)-farnesyldiphosphate specific]</fullName>
            <ecNumber evidence="5">2.5.1.87</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">wild type</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>n isopentenyl diphosphate + (2E,6E)-farnesyl diphosphate = a di-trans,poly-cis-polyprenyl diphosphate + n diphosphate</text>
            <dbReference type="Rhea" id="RHEA:53008"/>
            <dbReference type="Rhea" id="RHEA-COMP:19494"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="ChEBI" id="CHEBI:136960"/>
            <dbReference type="ChEBI" id="CHEBI:175763"/>
            <dbReference type="EC" id="2.5.1.87"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the UPP synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.87" evidence="5"/>
    <dbReference type="EMBL" id="EAAA01000212">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6Q301"/>
    <dbReference type="FunCoup" id="F6Q301">
        <property type="interactions" value="70"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000025006"/>
    <dbReference type="Ensembl" id="ENSCINT00000025252.2">
        <property type="protein sequence ID" value="ENSCINP00000025006.2"/>
        <property type="gene ID" value="ENSCING00000013665.2"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1172926_0_0_1"/>
    <dbReference type="InParanoid" id="F6Q301"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904423">
        <property type="term" value="C:dehydrodolichyl diphosphate synthase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045547">
        <property type="term" value="F:ditrans,polycis-polyprenyl diphosphate synthase [(2E,6E)-farnesyl diphosphate specific] activity"/>
        <property type="evidence" value="ECO:0007669"/>
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        <source>
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        <source>
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        <source>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
    <sequence length="237" mass="27365" checksum="487487A76F55B97F" modified="2012-04-18" version="2">MRILLFFVHHLRSKTNCCNDFWFNKMTSLLERKWTSVLNQPIHHVLNVVFIAVHGAVVMWRILKLVVMYLLSHQIKIPTLRNTSAETMQADGRKLTKTPLHLIIIIGEKEYNCDSIVDMLVWSFFIGIRYVTISSKSIKLRNQCQMRFEERCLKLSSTIITSKTKFHSDYMFATGNQDFNVVFTALKNSKAEFMNVCKEICTSKTRSSVIKLDSIDHLVKENTGNILEPDLGLCFGP</sequence>
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    <accession>F6QLP3</accession>
    <accession>A0A1W2W803</accession>
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        <name type="scientific">Ciona intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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    <reference evidence="7" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">wild type</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_002126524.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6QLP3"/>
    <dbReference type="Ensembl" id="ENSCINT00000013254.3">
        <property type="protein sequence ID" value="ENSCINP00000013254.3"/>
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        <property type="entry name" value="ZP_dom"/>
    </dbReference>
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    <dbReference type="PANTHER" id="PTHR14002:SF20">
        <property type="entry name" value="ZONA PELLUCIDA-LIKE DOMAIN-CONTAINING PROTEIN 1"/>
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        <location>
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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    <reference evidence="10" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">wild type</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="EAAA01001781">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6RC45"/>
    <dbReference type="STRING" id="7719.ENSCINP00000018311"/>
    <dbReference type="Ensembl" id="ENSCINT00000018311.3">
        <property type="protein sequence ID" value="ENSCINP00000018311.3"/>
        <property type="gene ID" value="ENSCING00000009016.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000167190"/>
    <dbReference type="HOGENOM" id="CLU_016750_2_1_1"/>
    <dbReference type="InParanoid" id="F6RC45"/>
    <dbReference type="OMA" id="HCACKES"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.220.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057244">
        <property type="entry name" value="GAIN_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046338">
        <property type="entry name" value="GAIN_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000203">
        <property type="entry name" value="GPS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45692:SF1">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 2 PROFILE 2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45692">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01825">
        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00249">
        <property type="entry name" value="GPCRSECRETIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00303">
        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50221">
        <property type="entry name" value="GAIN_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="141"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="174"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="213"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="245"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="300"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="350"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="382"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="domain" description="GAIN-B" evidence="7">
        <location>
            <begin position="1"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="8">
        <location>
            <begin position="139"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000018311.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="453" mass="50156" checksum="17DF376D51F05F9D" modified="2012-04-18" version="2">MNIALPLAVYERIRTHIQRVSFVVYKDKFLFPSNATVMSSVISAELPQVNTQGLSSPIVITANVKLPDNSSLNLIKCSFWNFTTLSWRSEGCEVANISSSVDERGYSTITCHCNHLTNFAVLLNHDTRENPTVEDNEITELISYAGIIASIIGLSITIIFRLSRREFRRGVPQIILLHLCFCLLIVYIMMLIYDKSFVVQHMGSCFLVGFLMHYFLLSSWGWCLTEGVSMYLLLVQVYNVNLRRFLTKATICCYGAASLLVCGSLILSGSSADVAEHPGYIVLDAYRYGDACVVTYPNSLYFVIIPIAVACSLNLVCFVFVIKGLRQTAASDGMGGTKKKNMVKHRLRQAVCVTTLLGVTWCIGFVKVALQSSFGTTSDVVWAFEILFSAVNSVQGFVIFLMFCAANLWSEFRCTTGPDKKRGRLYSQTSKLGSTSNEKVAAVTATTHQSSTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>F6RGX1</accession>
    <name>F6RGX1_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Ubiquitin-conjugating enzyme E2 J2</fullName>
            <ecNumber evidence="2">2.3.2.23</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">wild type</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the covalent attachment of ubiquitin to other proteins. Seems to function in the selective degradation of misfolded membrane proteins from the endoplasmic reticulum (ERAD). In cooperation with the GATOR2 complex, catalyzes 'Lys-6'-linked ubiquitination of NPRL2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="2"/>
    <dbReference type="EMBL" id="EAAA01000464">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6RGX1"/>
    <dbReference type="FunCoup" id="F6RGX1">
        <property type="interactions" value="255"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000011978"/>
    <dbReference type="Ensembl" id="ENSCINT00000011978.3">
        <property type="protein sequence ID" value="ENSCINP00000011978.3"/>
        <property type="gene ID" value="ENSCING00000005808.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156173"/>
    <dbReference type="HOGENOM" id="CLU_041481_1_3_1"/>
    <dbReference type="InParanoid" id="F6RGX1"/>
    <dbReference type="OMA" id="IPPHILM"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd23799">
        <property type="entry name" value="UBCc_UBE2J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000023">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 J2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="13">
        <location>
            <begin position="10"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00054775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00073320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000011978.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="181" mass="20711" checksum="5C945E4F54D6FA22" modified="2012-04-18" version="2">MSKRSKVPITATQRLKQDYMRLKKDPIPYITAEPLPSNILEWHYLVTGPDDTPYTGGFYHGKLVFPREFPFRPPAIYMITPSGRFKCNTRLCLSISDFHPDTWNPAWSVGTILTGLLSFMVEETPTLGSVESSDFTKRNLAVCSRLFNIQNKTFCELFPAITEKIRSSIEIRDSLTLRSQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>F6RW55</accession>
    <name>F6RW55_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="F6RW55"/>
    <dbReference type="Ensembl" id="ENSCINT00000028006.2">
        <property type="protein sequence ID" value="ENSCINP00000027760.2"/>
        <property type="gene ID" value="ENSCING00000015809.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000289650"/>
    <dbReference type="HOGENOM" id="CLU_1334531_0_0_1"/>
    <dbReference type="InParanoid" id="F6RW55"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="74"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="111"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="157"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000027760.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="206" mass="22900" checksum="9FBE1909B4D6CF42" modified="2011-07-27" version="1">MFATEKSSFSNSSGTNITNGINTRTEWILSTTFSAICFVSVFYLTTALSIYAQRKRLCICQGQLNGNGIKGPRLLSYLSIIGIMAEIIYFATGLIPLHLFGEPACSIGQRITISCGVLVLGCVYFGLWFRIYSIFYHDQIVKKNLHIVVRAIGKMSFLFFVVMLVTNTIVFMWAPAYKTTPAGCLKVEQKDSSTLKWSLLVSSTVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>F6S8U4</accession>
    <accession>A0A1W2WDI0</accession>
    <name>F6S8U4_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Aldehyde dehydrogenase family 8 member A1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100177969</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">wild type</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01000640">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002126639.1">
        <property type="nucleotide sequence ID" value="XM_002126603.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6S8U4"/>
    <dbReference type="FunCoup" id="F6S8U4">
        <property type="interactions" value="4"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000026640"/>
    <dbReference type="Ensembl" id="ENSCINT00000026886.2">
        <property type="protein sequence ID" value="ENSCINP00000026640.2"/>
        <property type="gene ID" value="ENSCING00000014829.2"/>
    </dbReference>
    <dbReference type="GeneID" id="100177969"/>
    <dbReference type="KEGG" id="cin:100177969"/>
    <dbReference type="GeneTree" id="ENSGT00940000156799"/>
    <dbReference type="HOGENOM" id="CLU_005391_0_2_1"/>
    <dbReference type="InParanoid" id="F6S8U4"/>
    <dbReference type="OMA" id="PMPIAAW"/>
    <dbReference type="OrthoDB" id="310895at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016620">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07093">
        <property type="entry name" value="ALDH_F8_HMSADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000001">
        <property type="entry name" value="Aldehyde dehydrogenase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000012">
        <property type="entry name" value="Betaine aldehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43720">
        <property type="entry name" value="2-AMINOMUCONIC SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43720:SF2">
        <property type="entry name" value="2-AMINOMUCONIC SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="2 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="6">
        <location>
            <begin position="15"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000026640.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="487" mass="53409" checksum="26D1A5001DEA0D15" modified="2012-04-18" version="2">MCDVKEIKNYINGVFVEPKSGKFIESLCPATGLINAKVPSSETSDVDDAVQSAKQAFYGWSNTSPQDRCNIMRRIADILESRLDEFAQLESQDQGKPVTLATSVDIPRAIYNFRFFASVILHETDDCTHLTNPTNALNYVHRMPVGVAGLISPWNLPLYLLTWKIAPAIAFGNTCVAKPSEFTSVTAWKLCEVFTQAELPPGVVNIVFGDGPTAGEAIVKHKDVSVISFTGSTAVGHHISEVVAPLCKPVSLELGGKNAALVFGDANLDDCVQCCVRSSFANQGEICLCTSRIYVDSTIYEEFVAKFVQRTKLLKVGDPDDKSTNVGALISKQHWEKVKHYINIARDDGGKIECGDGVEQLRLPQRVKGGFFIQPTVVTGVDDSSRCVQDEIFGPVVCIMPFNTEEEAVKRSNAVKYGLCATVWTSDLARAHRVSKKLEAGTVWVNCWMVRDLNMPFGGMKQSGIGRESAKHSAEFFTEAKTVCIKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>F6SF30</accession>
    <name>F6SF30_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CCR4-NOT transcription complex subunit 10</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
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                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
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                <person name="De Tomaso A."/>
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                <person name="Satake M."/>
                <person name="Terry A."/>
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                <person name="Yagi K."/>
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                <person name="Zhang C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <consortium name="Ensembl"/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="De Tomaso A."/>
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                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
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                <person name="Shin-i T."/>
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                <person name="Suzuki M.M."/>
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                <person name="Yagi K."/>
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                <person name="Wada S."/>
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                <person name="Larimer F."/>
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                <person name="Richardson P."/>
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                <person name="Kohara Y."/>
                <person name="Levine M."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">wild type</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01001070">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6SN53"/>
    <dbReference type="FunCoup" id="F6SN53">
        <property type="interactions" value="27"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000010376"/>
    <dbReference type="Ensembl" id="ENSCINT00000010375.3">
        <property type="protein sequence ID" value="ENSCINP00000010376.3"/>
        <property type="gene ID" value="ENSCING00000005039.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000173708"/>
    <dbReference type="HOGENOM" id="CLU_055721_4_2_1"/>
    <dbReference type="InParanoid" id="F6SN53"/>
    <dbReference type="OMA" id="CSKIRYQ"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016279">
        <property type="term" value="F:protein-lysine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Methyltransf_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614">
        <property type="entry name" value="HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614:SF109">
        <property type="entry name" value="RIBOSOMAL LYSINE N-METHYLTRANSFERASE 5"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Methyltransf_16"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003346505" evidence="3">
        <location>
            <begin position="20"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000010376.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="243" mass="27377" checksum="196FCC99A4671DC8" modified="2012-04-18" version="2">MPRILKCLIIYLMMSTTAGTLKCEETETNCNAISNYAVVPFNTNSVLSEFFERTKSVTISKLDVTFNILQTWQEHGVAGVVWEAATVLADYLADNYDFRGRNVIELGAGTGLVGMAVAYLGGNVTVTDLQKFLPLLQENVDLNKNIIEKGGNGGNLTISELKWGKRLERFKPGFYDFILGADIIYSEEEFQNLLETLTHLYGDDKNSKRKVILSAKRRYDRVETFIETLETKFRSVDLVKSCE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>F6SRA1</accession>
    <name>F6SRA1_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Tubulin polyglutamylase TTLL4-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100179385</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the tubulin--tyrosine ligase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="F6SRA1"/>
    <dbReference type="STRING" id="7719.ENSCINP00000013692"/>
    <dbReference type="Ensembl" id="ENSCINT00000013692.3">
        <property type="protein sequence ID" value="ENSCINP00000013692.3"/>
        <property type="gene ID" value="ENSCING00000006672.3"/>
    </dbReference>
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    <dbReference type="InParanoid" id="F6SRA1"/>
    <dbReference type="OMA" id="FWIGYWG"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015631">
        <property type="term" value="F:tubulin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070740">
        <property type="term" value="F:tubulin-glutamic acid ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000009">
        <property type="entry name" value="tubulin polyglutamylase TTLL5 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004344">
        <property type="entry name" value="TTL/TTLL_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12241:SF162">
        <property type="entry name" value="TUBULIN MONOGLUTAMYLASE TTLL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12241">
        <property type="entry name" value="TUBULIN POLYGLUTAMYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03133">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51221">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0493">Microtubule</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="305"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="306"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="321"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006820"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000013692.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="452" mass="52956" checksum="CEDC1AD7526AE03B" modified="2012-04-18" version="2">MKSNGFRALREYQKINHFPGSFQLGRKDRFWRNISRMQTRFGKKEFGFVPQTYIMPWDKKLLKNAWEEGSSKQKFILKPPASARGIGIRVIHKWNQVPLKKAVIVQKYLSRPYLINGSKFDLRLYVYVTSFDPLRIYLFEDGLVRFATCKYSSSMKHLSNRYMHLTNYSINKKSGEFQQNDDATVCQGHKWSLKALWGHLQEQGVNTDKIWEQMKDTVIKTIISVEQFTNSLVKSNCRRRYCCHELFGFDIMLDDKLRPWVLEVNISPSLHSNSPLDVEIKGPMVRDMFNLAGFLVPDKRQILANAPTPSTGTGAAASFSQDKRKQGQEISTDSRAKHTYFAQHHKQMDESELTSLMLDTLTINDIRILVESENENARCGEFQRIFPSVNKKYLHYFEHPRYYNFLLHTWEQKYRHQHMRGYSLLESFCRKGLHLPAELTHVPPDHIWSVST</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>F6SYC7</accession>
    <accession>A0A1W2W6I7</accession>
    <name>F6SYC7_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Alpha-N-acetylgalactosamine-specific lectin-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100179025</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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                <person name="Satou Y."/>
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                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
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                <person name="Makabe K.W."/>
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                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
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                <person name="Nishizaka S."/>
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                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
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                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
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                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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        <property type="entry name" value="Nucleic acid-binding proteins"/>
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        <property type="entry name" value="NA-bd_OB-fold"/>
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        <property type="entry name" value="Ribosomal_uL2_RNA-bd_dom"/>
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        <property type="entry name" value="Ribosomal_L2"/>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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            <begin position="104"/>
            <end position="181"/>
        </location>
    </feature>
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            <dbReference type="SMART" id="SM01383"/>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="219" mass="24732" checksum="59A787116D26DA40" modified="2012-04-18" version="2">MFTQTVKLVTQKLPTVSSSLTFNVRNLRTLPCANSKLNLNIFGPPANLPTEKHYHTSTALNGRYGHRLVKLWEQPEYTTKPLKVYRTGGRLPTSRFGSDEKVPGKKWTSKIGGGLPRQWYWVDNNRLTFEEVKNEVIFQERILKVMEDDNRSGHIALVAGPLKQRWILATETMKPGQLITNCATELPGGSWFKFIIRLIDLVQGRATSPVGGPDMGKIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>F6THQ0</accession>
    <name>F6THQ0_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">wild type</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="EAAA01001962">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6THQ0"/>
    <dbReference type="Ensembl" id="ENSCINT00000002939.3">
        <property type="protein sequence ID" value="ENSCINP00000002939.3"/>
        <property type="gene ID" value="ENSCING00000001492.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165427"/>
    <dbReference type="HOGENOM" id="CLU_1610179_0_0_1"/>
    <dbReference type="InParanoid" id="F6THQ0"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24228">
        <property type="entry name" value="B2 BRADYKININ RECEPTOR/ANGIOTENSIN II RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24228:SF74">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 1 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="86"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="122"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="10">
        <location>
            <begin position="1"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000002939.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="165" mass="19409" checksum="3049D0A9148EAF22" modified="2012-04-18" version="2">MQVLRVLFQFLPIVTMVVLYSLMFWRLKKKWGDENNGLPWEKQSKNGPDAVELMAMNERGVKLNEEEAKEARRQVVERKKLTEKRLLTVSVVICCAFVFLFLPSLIVNLISGSENLDPRVHMITSNITWLNSCVNPFIYVILNPDFRMEYRIILKYLWLKITCKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>F6TNU2</accession>
    <name>F6TNU2_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Deoxyribose-phosphate aldolase</fullName>
            <ecNumber evidence="5">4.1.2.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">2-deoxy-D-ribose 5-phosphate aldolase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Phosphodeoxyriboaldolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC100176285</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Catalyzes a reversible aldol reaction between acetaldehyde and D-glyceraldehyde 3-phosphate to generate 2-deoxy-D-ribose 5-phosphate. Participates in stress granule (SG) assembly. May allow ATP production from extracellular deoxyinosine in conditions of energy deprivation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>2-deoxy-D-ribose 5-phosphate = D-glyceraldehyde 3-phosphate + acetaldehyde</text>
            <dbReference type="Rhea" id="RHEA:12821"/>
            <dbReference type="ChEBI" id="CHEBI:15343"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="ChEBI" id="CHEBI:62877"/>
            <dbReference type="EC" id="4.1.2.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Carbohydrate degradation; 2-deoxy-D-ribose 1-phosphate degradation; D-glyceraldehyde 3-phosphate and acetaldehyde from 2-deoxy-alpha-D-ribose 1-phosphate: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Interacts with YBX1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic granule</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the DeoC/FbaB aldolase family. DeoC type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.4" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="F6TNU2"/>
    <dbReference type="FunCoup" id="F6TNU2">
        <property type="interactions" value="427"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000017567"/>
    <dbReference type="Ensembl" id="ENSCINT00000017567.3">
        <property type="protein sequence ID" value="ENSCINP00000017567.3"/>
        <property type="gene ID" value="ENSCING00000008612.3"/>
    </dbReference>
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    <sequence length="318" mass="34221" checksum="D1A1899C5128B20C" modified="2012-04-18" version="2">MAYNPGMELDMGWVNGAVVDLPAVNRRAATLLTKRTVKKSWQAAWLLRAVTCIDLTTLSGDDTVTNVQRLCFKACNPIRQDLLDAMGMGDYGITVGAVCVYSARVKDAVQSLKQAGGAAIPIASVATGFPAGQVPFETRLKEISMAIGLGATEIDIVISRDLALSGDWGKVYEEIKACKAACGENVHLKTILGTGDLGTLTNVYKASLVAMMAGSDFIKTSTGKEGVNATMPVGLTMVRAIREYFHKTGFKVGFKPAGGIRTAKDSCAWLALMKEELGDEWMRADLFRIGASSLLTDIERQLYHFVTGRYAAAHQLPM</sequence>
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        <name type="scientific">Ciona intestinalis</name>
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            <taxon>Phlebobranchia</taxon>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="entry name" value="Head and neck region of the ectodomain of NDV fusion glycoprotein"/>
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    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="UNNAMED PRODUCT"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <location>
            <begin position="153"/>
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        <location>
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        <location>
            <begin position="153"/>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    </evidence>
    <sequence length="380" mass="41916" checksum="A803D6F8422B7760" modified="2011-07-27" version="1">MYEIYTTIEKRPSEIRFSQDSIHHKFRRRSGHGEDNVNDTIDKILSGRLLATDLPRIEVAERDGELYTLGNRRLYMFRVLELKGKLKTIRVNVVSSLRRGLTTINDGVSIYMRNGGGTRDHAHAAPIPKPKEIVHKLLDELLLQVINSASQHTTNEAGTSMSVSKVVPDGSKAKSTDVVGKNSDDAIKTDGAISESSEGVVTTYHDVIISSDVAVVDAAVYEVCSDLNKSFEKVNKPNNLSDGDVETQNDDVAIRDDDVVTRDDDVVTKAHDVETQDDDVVTKNDNVETKVDGVVTKANDVETQVDDVETQVDDVETQVDDVVTKVDDVETKDVIPSFDSDANIFSVEKDRTSHQLGKRRHVELADTASAGEPEAKRSKT</sequence>
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    <accession>F6VWA2</accession>
    <accession>A0A1W2WCE8</accession>
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        <submittedName>
            <fullName evidence="3">Coiled-coil domain-containing protein 89-like</fullName>
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        <name evidence="3" type="primary">LOC100179709</name>
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    <organism evidence="3 4">
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        <lineage>
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            <taxon>Ciona</taxon>
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    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GOLGA6 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002124728.2">
        <property type="nucleotide sequence ID" value="XM_002124692.5"/>
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    <dbReference type="AlphaFoldDB" id="F6VWA2"/>
    <dbReference type="Ensembl" id="ENSCINT00000027924.2">
        <property type="protein sequence ID" value="ENSCINP00000027678.2"/>
        <property type="gene ID" value="ENSCING00000015730.2"/>
    </dbReference>
    <dbReference type="GeneID" id="100179709"/>
    <dbReference type="KEGG" id="cin:100179709"/>
    <dbReference type="GeneTree" id="ENSGT01050000248019"/>
    <dbReference type="HOGENOM" id="CLU_930516_0_0_1"/>
    <dbReference type="InParanoid" id="F6VWA2"/>
    <dbReference type="OMA" id="QEVRCEN"/>
    <dbReference type="OrthoDB" id="9449337at2759"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026737">
        <property type="entry name" value="GOLGA6L"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23143:SF30">
        <property type="entry name" value="SPERMATID ASSOCIATED LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23143">
        <property type="entry name" value="TRICHOHYALIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="111"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000027678.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="336" mass="39400" checksum="000BE63495A7336D" modified="2012-04-18" version="2">MTSSALTTYHNSSPSHQTWDSALADVLSVLRIQYRNDDDVMLFLTEPDKLKQLQYSTAAKMEWNRRVIKEEMDSGIHSPPRSRRIAKEVAIDYIRRYLDGEEERMDLVRKIQVVTSERNAAMESVIELEREMKRLKDESVASNCENDNLKGDIEKLRTDVAAFRTDNQEFRTENLKTKEENEHLRGENTKLRDENKKLRLENQDLRNENGALRSENQKVRGDNVKLRDETGDMRSENMKLRNENQAVRDENQEVRCENLKVRDNNLSLRGENDRFRSVNEALLSENKRYTRDTLELNAREDEMRMLVNVMQSLLAGKGEMRSESIRVDEQAEEEDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>F6W453</accession>
    <name>F6W453_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cell division cycle 5-like protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">wild type</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">DNA-binding protein involved in cell cycle control. May act as a transcription activator. Plays a role in pre-mRNA splicing as core component of precatalytic, catalytic and postcatalytic spliceosomal complexes. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. The PRP19-CDC5L complex may also play a role in the response to DNA damage (DDR). As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CEF1 family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01000286">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6W453"/>
    <dbReference type="FunCoup" id="F6W453">
        <property type="interactions" value="871"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000016360"/>
    <dbReference type="Ensembl" id="ENSCINT00000016360.3">
        <property type="protein sequence ID" value="ENSCINP00000016360.3"/>
        <property type="gene ID" value="ENSCING00000007995.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00550000074922"/>
    <dbReference type="HOGENOM" id="CLU_009082_0_0_1"/>
    <dbReference type="InParanoid" id="F6W453"/>
    <dbReference type="OMA" id="KMGMAGE"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11659">
        <property type="entry name" value="SANT_CDC5_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000021">
        <property type="entry name" value="CDC5 cell division cycle 5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000091">
        <property type="entry name" value="CDC5 cell division cycle 5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047242">
        <property type="entry name" value="CDC5L/Cef1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021786">
        <property type="entry name" value="Cdc5p/Cef1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047240">
        <property type="entry name" value="SANT_CDC5L_II"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45885">
        <property type="entry name" value="CELL DIVISION CYCLE 5-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45885:SF1">
        <property type="entry name" value="CELL DIVISION CYCLE 5-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11831">
        <property type="entry name" value="Myb_Cef"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13921">
        <property type="entry name" value="Myb_DNA-bind_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6 14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="HTH myb-type" evidence="17">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="16">
        <location>
            <begin position="3"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="16">
        <location>
            <begin position="55"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="17">
        <location>
            <begin position="59"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="111"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="384"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="244"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="667"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="118"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00057681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00074456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000016360.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="779" mass="88813" checksum="76BF51EEC8966E34" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F6WKJ4</accession>
    <name>F6WKJ4_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
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                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
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                <person name="Sasaki A."/>
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                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
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                <person name="Doggett N."/>
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                <person name="Richardson P."/>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
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                <person name="De Tomaso A."/>
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                <person name="Putnam N."/>
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                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
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                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
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                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">wild type</strain>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
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                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
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                <person name="Hastings K.E."/>
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                <person name="Meinertzhagen I.A."/>
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                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
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                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">wild type</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>3-(carbamoylamino)-2-methylpropanoate + H2O + 2 H(+) = (R)-3-amino-2-methylpropanoate + NH4(+) + CO2</text>
            <dbReference type="Rhea" id="RHEA:37339"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57731"/>
            <dbReference type="ChEBI" id="CHEBI:74414"/>
            <dbReference type="EC" id="3.5.1.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>3-(carbamoylamino)propanoate + H2O + 2 H(+) = beta-alanine + NH4(+) + CO2</text>
            <dbReference type="Rhea" id="RHEA:11184"/>
            <dbReference type="ChEBI" id="CHEBI:11892"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57966"/>
            <dbReference type="EC" id="3.5.1.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; beta-alanine biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the carbon-nitrogen hydrolase superfamily. BUP family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.6" evidence="6"/>
    <dbReference type="EMBL" id="EAAA01001207">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="EAAA01001208">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6WQG9"/>
    <dbReference type="FunCoup" id="F6WQG9">
        <property type="interactions" value="18"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000011775"/>
    <dbReference type="Ensembl" id="ENSCINT00000011775.3">
        <property type="protein sequence ID" value="ENSCINP00000011775.3"/>
        <property type="gene ID" value="ENSCING00000010868.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000004906"/>
    <dbReference type="HOGENOM" id="CLU_030130_4_1_1"/>
    <dbReference type="InParanoid" id="F6WQG9"/>
    <dbReference type="OMA" id="HWPFLRD"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003837">
        <property type="term" value="F:beta-ureidopropionase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033396">
        <property type="term" value="P:beta-alanine biosynthetic process via 3-ureidopropionate"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07587">
        <property type="entry name" value="ML_beta-AS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.110.10:FF:000008">
        <property type="entry name" value="Beta-alanine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050345">
        <property type="entry name" value="Aliph_Amidase/BUP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43674:SF2">
        <property type="entry name" value="BETA-UREIDOPROPIONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43674">
        <property type="entry name" value="NITRILASE C965.09-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CN hydrolase" evidence="9">
        <location>
            <begin position="69"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00050540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00050552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00061249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00066985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00074804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00075038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000011775.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="388" mass="43350" checksum="5224BD4D936AEBBF" modified="2012-04-18" version="2">AEKMELESVEDTLNKYIPPAELTEVKRILYGPEFLRTLQIEKQAGDIANNEDFELAAYEMPLSQNPRQVKIGLIQNKIISPTDAPVSTQIHSLHERISKIAEAAALSGVNILCMQEAWTMPFAFCTREKIPWTDFAESAENGATTKLCQELARKHNMVVVSPILERDETHGGVLWNTAVVISNTGAVMGKSRKNHIPRVGDFNESTYYMEGNTGHKVFQTSFGRIGINICYGRHHPLNWMMYGINGAEIVFNPSATVGALSEPMWPIEARCAAIANHYYACGINRVGTETFSNEFTSGDGKKAHKDFGHFYGSSYIASPDGSRTPGLSRTKDGLLVVAVDLNICQEISDKWCFRMTARHELYADAIKQSTELDYKRDIVYENSLSFLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>F6WWW9</accession>
    <name>F6WWW9_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">5'-nucleotidase</fullName>
            <ecNumber evidence="3 9">3.1.3.5</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">wild type</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>a ribonucleoside 5'-phosphate + H2O = a ribonucleoside + phosphate</text>
            <dbReference type="Rhea" id="RHEA:12484"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:18254"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58043"/>
            <dbReference type="EC" id="3.1.3.5"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the pyrimidine 5'-nucleotidase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.5" evidence="3 9"/>
    <dbReference type="EMBL" id="EAAA01001687">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6WWW9"/>
    <dbReference type="FunCoup" id="F6WWW9">
        <property type="interactions" value="108"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000019385"/>
    <dbReference type="Ensembl" id="ENSCINT00000019385.3">
        <property type="protein sequence ID" value="ENSCINP00000019385.3"/>
        <property type="gene ID" value="ENSCING00000009520.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000012959"/>
    <dbReference type="HOGENOM" id="CLU_048584_0_2_1"/>
    <dbReference type="InParanoid" id="F6WWW9"/>
    <dbReference type="OMA" id="HIPWIYL"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008253">
        <property type="term" value="F:5'-nucleotidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.340:FF:000001">
        <property type="entry name" value="Cytosolic 5-nucleotidase 3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000032">
        <property type="entry name" value="Cytosolic 5-nucleotidase 3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.340">
        <property type="entry name" value="Pyrimidine 5'-nucleotidase (UMPH-1), N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006434">
        <property type="entry name" value="Pyrimidine_nucleotidase_eu"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01544">
        <property type="entry name" value="HAD-SF-IE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13045">
        <property type="entry name" value="5'-NUCLEOTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13045:SF0">
        <property type="entry name" value="7-METHYLGUANOSINE PHOSPHATE-SPECIFIC 5'-NUCLEOTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05822">
        <property type="entry name" value="UMPH-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01128">
        <property type="entry name" value="C1.4:_5'-Nucleotidase_Like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 9" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000019385.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="288" mass="32749" checksum="043B70D29DF2614A" modified="2012-04-18" version="2">KMREEKGGKIFIKDEDVVSKKIQNLIDGGVDSFQVISDFDRTLSRASYNGEKCTTCHGILESSIVFNEETQKDLAALKKRFYAIEIDPTMTIEEKCPYMVEWWETAHKIMIKSKVKRTSIQQAVTESNVILKDNCKEMFQILNQNSVPLLIFSAGIADVLMEVLYQKSSFLPNIKVVSNFMKFDENGVLVGFEGELIHVYNKNEGALTDSAYWYFEDIKHRHNICLLGDSMGDLHMADGVPDVNTCLKIGYLNEKVEHLLPRYIDAWDIVLVDDPTMDVANVLFAAIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>F6X3J8</accession>
    <name>F6X3J8_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Coiled-coil domain-containing protein 181</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100180402</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Microtubule-binding protein that localizes to the microtubular manchette of elongating spermatids.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Homodimer. Interacts with HOOK1. Interacts with HOOK2. Interacts with HOOK3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Cilium</location>
            <location evidence="2">Flagellum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CCDC181 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="F6X3J8"/>
    <dbReference type="FunCoup" id="F6X3J8">
        <property type="interactions" value="5"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000027943"/>
    <dbReference type="Ensembl" id="ENSCINT00000028189.2">
        <property type="protein sequence ID" value="ENSCINP00000027943.2"/>
        <property type="gene ID" value="ENSCING00000015965.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000018244"/>
    <dbReference type="HOGENOM" id="CLU_040811_0_0_1"/>
    <dbReference type="InParanoid" id="F6X3J8"/>
    <dbReference type="OMA" id="KAWLMRK"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026687">
        <property type="entry name" value="CCDC181"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14320">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 181"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14320:SF2">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 181"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-0969">Cilium</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0282">Flagellum</keyword>
    <keyword evidence="7" id="KW-0493">Microtubule</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="44"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="256"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="373"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="45"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="93"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="105"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="127"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="149"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="182"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="195"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="279"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="373"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000027943.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="496" mass="58410" checksum="0BD0BDDF1DFDFFAD" modified="2012-04-18" version="2">KYEGEMRDSKMEESHEKDWEKDLGWLLKDSDKTEKVIEMFLKFHQQNDENEKENQKEFEKRENTSDGDVITQGIIHVIDYDITGEERTSDAPTTDDDDDDGLTLEGRRELEENTLRNDADEGEEYNVLERLEKANEALKNDSMDESESERERRVIFKPKLVDFEAPPQDYSSSSSSSSDDEENRKVTKTMEDMRLTQQGNETVEINGQKIRKDEKVLIERDGKFELVGASELKAILPPVNGGHPDNVAVVNGNHYNPLPPVKPRPKSAPVRSSKPHPPTSNYRPFSANNAPTTPSSNYTAKVKKILKIYIYIYIYTFCYSPELKELMRKRAAAKAAREREEEARRKAEEESARQEAEYAWKCWLERKNEQIKEQRKRERQVAERRRKDNEKNPNEAAEAYSTWLKDKRKQTKKERIEVQQQQIEIDEGWYIRERAECRKAFRQWLRKKNEAARQAQMQDKLLRRQSLKQAREARRAQKLLESIRESQQMKYVEYYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>F6XI02</accession>
    <name>F6XI02_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">U-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01001485">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="Ensembl" id="ENSCINT00000022162.2">
        <property type="protein sequence ID" value="ENSCINP00000021916.2"/>
        <property type="gene ID" value="ENSCING00000010094.3"/>
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    <dbReference type="GeneTree" id="ENSGT00510000049555"/>
    <dbReference type="HOGENOM" id="CLU_1051820_0_0_1"/>
    <dbReference type="InParanoid" id="F6XI02"/>
    <dbReference type="OMA" id="PNEAINN"/>
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        <property type="component" value="Chromosome 2"/>
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    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
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        <property type="entry name" value="RING FINGER PROTEIN 37"/>
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    </dbReference>
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        <property type="entry name" value="RING FINGER PROTEIN 37"/>
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        <property type="entry name" value="U-box"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="U-box" evidence="1">
        <location>
            <begin position="229"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000021916.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="259" mass="28617" checksum="9A5CFCCAE6DF2AB1" modified="2012-04-18" version="2">MSMNFCHLYLSTTITSSCLCSDGYEAENLLSSTQPNGFQAERFARPPAYITVTFPIPIQLDRITVRPWVGQQCVKDLQLYIARINRSNALKSVSPDEVKYKAGRAVLSTPVEVSFVNKHYRAVRCLEKGENEVKQSGIIVDVQGNMDGILAIKLGIISMLTGTVPSIGGLSIWGKPSNKCKRNLANSVIQKWLQNQNLPQCTLINNSITVLPTLTNKDESDNESADKTEIPKQFVDPIVCSVMRNPVLLPSGHTVDQHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>F6XI30</accession>
    <name>F6XI30_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CUB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">wild type</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001485">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6XI30"/>
    <dbReference type="STRING" id="7719.ENSCINP00000021913"/>
    <dbReference type="Ensembl" id="ENSCINT00000022159.2">
        <property type="protein sequence ID" value="ENSCINP00000021913.2"/>
        <property type="gene ID" value="ENSCING00000015952.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166030"/>
    <dbReference type="HOGENOM" id="CLU_015228_4_0_1"/>
    <dbReference type="InParanoid" id="F6XI30"/>
    <dbReference type="OMA" id="ATAYICA"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000005">
        <property type="entry name" value="Procollagen C-endopeptidase enhancer 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24251:SF30">
        <property type="entry name" value="MEMBRANE FRIZZLED-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24251">
        <property type="entry name" value="OVOCHYMASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003350631" description="CUB domain-containing protein" evidence="4">
        <location>
            <begin position="23"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="5">
        <location>
            <begin position="23"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="5">
        <location>
            <begin position="148"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000021913.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="200" mass="21650" checksum="6480470342CCC7ED" modified="2011-07-27" version="1">MKQFIFSAFIFLLLTGIKDASACSMLGLTGGAGYSSPFISASTHPNNNPHNLNCYYRINAQSSSNVIKVNFRNVNVEVGSCCNTDYFAVYEGSSATSRLIGKYCGQHSSIVLQGRGQYMYIHYHTDGSVYSTGFELQFTVSASATPSCDQLSIGGGPGTGFITSPNYPSNYPHQADCRYRINAGSATRTITLTFTAFNLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>F6XNC0</accession>
    <name>F6XNC0_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">wild type</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Membrane</location>
            <topology evidence="1 13">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the integrin alpha chain family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001945">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6XNC0"/>
    <dbReference type="FunCoup" id="F6XNC0">
        <property type="interactions" value="20"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000024886"/>
    <dbReference type="Ensembl" id="ENSCINT00000025132.2">
        <property type="protein sequence ID" value="ENSCINP00000024886.2"/>
        <property type="gene ID" value="ENSCING00000013582.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000167229"/>
    <dbReference type="HOGENOM" id="CLU_004111_1_0_1"/>
    <dbReference type="InParanoid" id="F6XNC0"/>
    <dbReference type="OMA" id="AKKQWIT"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008305">
        <property type="term" value="C:integrin complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007229">
        <property type="term" value="P:integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.930">
        <property type="entry name" value="Bicelle-embedded integrin alpha(iib) transmembrane segment"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.130">
        <property type="entry name" value="Integrin alpha, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1460">
        <property type="entry name" value="Integrin domains. Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1510">
        <property type="entry name" value="ntegrin, alpha v. Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1530">
        <property type="entry name" value="ntegrin, alpha v. Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013517">
        <property type="entry name" value="FG-GAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013519">
        <property type="entry name" value="Int_alpha_beta-p"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000413">
        <property type="entry name" value="Integrin_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013649">
        <property type="entry name" value="Integrin_alpha_Ig-like_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048285">
        <property type="entry name" value="Integrin_alpha_Ig-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048286">
        <property type="entry name" value="Integrin_alpha_Ig-like_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028994">
        <property type="entry name" value="Integrin_alpha_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032695">
        <property type="entry name" value="Integrin_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23220">
        <property type="entry name" value="INTEGRIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23220:SF122">
        <property type="entry name" value="INTEGRIN ALPHA-PS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01839">
        <property type="entry name" value="FG-GAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08441">
        <property type="entry name" value="Integrin_A_Ig_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20805">
        <property type="entry name" value="Integrin_A_Ig_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20806">
        <property type="entry name" value="Integrin_A_Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01185">
        <property type="entry name" value="INTEGRINA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00191">
        <property type="entry name" value="Int_alpha"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69318">
        <property type="entry name" value="Integrin alpha N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69179">
        <property type="entry name" value="Integrin domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51470">
        <property type="entry name" value="FG_GAP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 13" id="KW-0401">Integrin</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 13" id="KW-0732">Signal</keyword>
    <keyword evidence="3 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5001424478" evidence="13">
        <location>
            <begin position="19"/>
            <end position="1045"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1014"/>
            <end position="1036"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="12">
        <location>
            <begin position="23"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="12">
        <location>
            <begin position="292"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="12">
        <location>
            <begin position="356"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="12">
        <location>
            <begin position="419"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="domain" description="Integrin alpha first immunoglubulin-like" evidence="14">
        <location>
            <begin position="466"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="domain" description="Integrin alpha second immunoglobulin-like" evidence="15">
        <location>
            <begin position="633"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="domain" description="Integrin alpha third immunoglobulin-like" evidence="16">
        <location>
            <begin position="793"/>
            <end position="998"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF20805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF20806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000024886.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="1045" mass="114298" checksum="645C08AD004B1AF8" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>F6XPD3</accession>
    <name>F6XPD3_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Nuclear respiratory factor 1 NLS/DNA-binding dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NRF1/Ewg family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="F6XPD3"/>
    <dbReference type="FunCoup" id="F6XPD3">
        <property type="interactions" value="46"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000029422"/>
    <dbReference type="Ensembl" id="ENSCINT00000029668.2">
        <property type="protein sequence ID" value="ENSCINP00000029422.2"/>
        <property type="gene ID" value="ENSCING00000017347.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000006835"/>
    <dbReference type="HOGENOM" id="CLU_018156_0_0_1"/>
    <dbReference type="InParanoid" id="F6XPD3"/>
    <dbReference type="OMA" id="PELPWAN"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039142">
        <property type="entry name" value="NRF1/Ewg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019525">
        <property type="entry name" value="Nrf1_NLS/DNA-bd_dimer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20338">
        <property type="entry name" value="NUCLEAR RESPIRATORY FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10491">
        <property type="entry name" value="Nrf1_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Nuclear respiratory factor 1 NLS/DNA-binding dimerisation" evidence="8">
        <location>
            <begin position="53"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="266"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="267"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="282"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF10491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000029422.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="392" mass="43420" checksum="BEC46C890A496A7B" modified="2012-04-18" version="2">MDSNFSRLLNLPVNLSMMSGNELEDDESFSASELLHSSAQDGISSSLVNTGTMPGLAAAAALVTSRKRHQPYAFELNPSIRKRQYNRLLRKLKQTIHELCTRCGQQAVVLCVSPGKENGGFRVIGSSPLDSVVKNCKSAIYQELEQELQEKLGPKREKTSDVLCDLPELLIEGIPTPVEKMNQAQLRMFIPEMLKHATGRGKPGWGKESTRPVWWPLDVPWANVRRDVRAPEVKIRVSWTQALRQIVKNCYRFHAREDLLVGMECETDGKNETGSPRDRQNRSNYNSPLSPPTLVQTVHNADGSVSLVQLDSGQTIATITQDQSGTAVATLTGQQMAGEIDMAMDDIEHGDWSLGEHDATIGEAFNVIASSHQEVVSQQKNDLSLTFDPDQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>F6XPZ4</accession>
    <name>F6XPZ4_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">TATA-box-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100177214</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">wild type</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TBP family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01000435">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="F6XPZ4"/>
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    <dbReference type="STRING" id="7719.ENSCINP00000005464"/>
    <dbReference type="Ensembl" id="ENSCINT00000005464.3">
        <property type="protein sequence ID" value="ENSCINP00000005464.3"/>
        <property type="gene ID" value="ENSCING00000002679.3"/>
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    <dbReference type="HOGENOM" id="CLU_060161_1_3_1"/>
    <dbReference type="InParanoid" id="F6XPZ4"/>
    <dbReference type="OMA" id="PQGHSIH"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 10"/>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="TBP_dom_sf"/>
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    <dbReference type="InterPro" id="IPR033710">
        <property type="entry name" value="TBP_eukaryotic"/>
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        <property type="entry name" value="TATA-BOX BINDING PROTEIN"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="TBP"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="TIFACTORIID"/>
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        <property type="entry name" value="TATA-box binding protein-like"/>
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        <property type="entry name" value="TFIID"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="93"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="15"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="93"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005560"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000005464.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="316" mass="35300" checksum="AE24CFF527E0C34B" modified="2012-04-18" version="2">MNPQGHSIHGMPGTIPNSSGNRQQMSSNPSLTHHQLTNVMPHSQPHNTFSHQMGGNSLHLPNPSGYSQQMQPHHQIGYKQTYATKNITDNNEQTNEQQRQDTLQTTSRSQIQGPPSISTPYSSVHPMTPRTPAEFGVGPPQLQNIVSTVNLDTKLDLKRIALHARNAEYNPKRFAAVIMRIREPRTTALIFSSGKMVCTGAKSENASRLAARKYARVVQKLGFPAKFIDFKIQNMVGSVDVKFPIRLEGLVLAHSQFSSYEPELFPGLIYRMVKPRIVLLIFVSGKVVLTGAKVYNEIEEAFNNIYPILKGYRKTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>F6XS01</accession>
    <name>F6XS01_CIOIN</name>
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        <recommendedName>
            <fullName evidence="9">Nuclear factor 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
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            <taxon>Ciona</taxon>
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    <reference evidence="13" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">wild type</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 9">Binds DNA as a homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the CTF/NF-I family.</text>
    </comment>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6XS01"/>
    <dbReference type="STRING" id="7719.ENSCINP00000005158"/>
    <dbReference type="Ensembl" id="ENSCINT00000005158.3">
        <property type="protein sequence ID" value="ENSCINP00000005158.3"/>
        <property type="gene ID" value="ENSCING00000002528.3"/>
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    <dbReference type="HOGENOM" id="CLU_012576_3_1_1"/>
    <dbReference type="InParanoid" id="F6XS01"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 5"/>
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    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="entry name" value="CTF/NFI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020604">
        <property type="entry name" value="CTF/NFI_DNA-bd-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019739">
        <property type="entry name" value="CTF/NFI_DNA-bd_CS"/>
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        <property type="entry name" value="CTF/NFI_DNA-bd_N"/>
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    <dbReference type="InterPro" id="IPR003619">
        <property type="entry name" value="MAD_homology1_Dwarfin-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11492">
        <property type="entry name" value="NUCLEAR FACTOR I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11492:SF8">
        <property type="entry name" value="NUCLEAR FACTOR I, ISOFORM B"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00859">
        <property type="entry name" value="CTF_NFI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03165">
        <property type="entry name" value="MH1"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF10524">
        <property type="entry name" value="NfI_DNAbd_pre-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00523">
        <property type="entry name" value="DWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00349">
        <property type="entry name" value="CTF_NFI_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51080">
        <property type="entry name" value="CTF_NFI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0010">Activator</keyword>
    <keyword evidence="3 9" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="CTF/NF-I" evidence="11">
        <location>
            <begin position="22"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="297"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="353"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="385"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000005158.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="433" mass="49637" checksum="FD00A656A4762659" modified="2012-04-18" version="1">EPVRRSYSAGTAGIDAIFPQADIQHRSWSQDEFHPFIEALMPHVKSFAYTWFNLQARKRKYFKKHEKRMSPSEEQAVKEELLREKPEVKQKWASRLLAKLRKDIRPEYREDFVLTITGKKSVCCILSNPDQKGKIRRIDCLRQADKVWRLDLVMIILFRGLPLESTDGERLCKSHACGQNAGLCVQPYHISVTVKELDLYLANYVKDEDSLKHASKIYKKNAKMLKTQNFNRKVVGATRAFKTSGVFGVHEIYRIAKTPIISSDIHNIGMGNSIESSSYYYKLRPRQRRHIPLCSSFKHSQTKRLKSSGSSMSAEEVNGIESGGENDAETFYNRPPSSHHSWHERSHVLPKPQSYSRTQKQNACQRSPLQSPTIKTTRAQPDLNGGHSSVTSPGSHSSPWHPVLQQSHHFPYGATQSFYHPRQDLKDFAQFAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>F6XU15</accession>
    <name>F6XU15_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B</fullName>
            <ecNumber evidence="6">2.4.99.18</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">wild type</strain>
        </source>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a di-trans,poly-cis-dolichyl diphosphooligosaccharide + L-asparaginyl-[protein] = N(4)-(oligosaccharide-(1-&gt;4)-N-acetyl-beta-D-glucosaminyl-(1-&gt;4)-N-acetyl-beta-D-glucosaminyl)-L-asparaginyl-[protein] + a di-trans,poly-cis-dolichyl diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22980"/>
            <dbReference type="Rhea" id="RHEA-COMP:12804"/>
            <dbReference type="Rhea" id="RHEA-COMP:12805"/>
            <dbReference type="Rhea" id="RHEA-COMP:19506"/>
            <dbReference type="Rhea" id="RHEA-COMP:19509"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:50347"/>
            <dbReference type="ChEBI" id="CHEBI:57497"/>
            <dbReference type="ChEBI" id="CHEBI:57570"/>
            <dbReference type="ChEBI" id="CHEBI:132529"/>
            <dbReference type="EC" id="2.4.99.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="18">Component of the oligosaccharyltransferase (OST) complex. There are 2 OST complexes, OST-A and OST-B, which contain STT3A or STT3B as catalytic subunit, respectively. OST-A and OST-B contain common core subunits RPN1, RPN2, OST48, OST4, DAD1 and TMEM258, and OST-B contains either MAGT1 or TUSC3 as specific accessory subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the STT3 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.99.18" evidence="6"/>
    <dbReference type="EMBL" id="EAAA01000305">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6XU15"/>
    <dbReference type="FunCoup" id="F6XU15">
        <property type="interactions" value="689"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000003935"/>
    <dbReference type="Ensembl" id="ENSCINT00000003935.3">
        <property type="protein sequence ID" value="ENSCINP00000003935.3"/>
        <property type="gene ID" value="ENSCING00000001945.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155488"/>
    <dbReference type="HOGENOM" id="CLU_009279_1_0_1"/>
    <dbReference type="InParanoid" id="F6XU15"/>
    <dbReference type="OMA" id="TKELWSP"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008250">
        <property type="term" value="C:oligosaccharyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004579">
        <property type="term" value="F:dolichyl-diphosphooligosaccharide-protein glycotransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043687">
        <property type="term" value="P:post-translational protein modification"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018279">
        <property type="term" value="P:protein N-linked glycosylation via asparagine"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12610:FF:000001">
        <property type="entry name" value="Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12610">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003674">
        <property type="entry name" value="Oligo_trans_STT3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048999">
        <property type="entry name" value="STT3-PglB_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048307">
        <property type="entry name" value="STT3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13872">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13872:SF1">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT STT3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02516">
        <property type="entry name" value="STT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21436">
        <property type="entry name" value="STT3-PglB_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="16" id="KW-0464">Manganese</keyword>
    <keyword evidence="14 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="20"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="89"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="121"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="145"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="176"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="198"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="243"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="274"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="304"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="366"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="392"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="416"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="501"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="domain" description="Oligosaccharyl transferase STT3 N-terminal" evidence="22">
        <location>
            <begin position="24"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="domain" description="STT3/PglB/AglB core" evidence="23">
        <location>
            <begin position="564"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="446"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="20">
        <location>
            <begin position="448"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
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        </location>
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    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="503"/>
            <end position="526"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="532"/>
            <end position="553"/>
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    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="117"/>
            <end position="558"/>
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    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
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    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="32"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="609"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="626"/>
            <end position="647"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
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    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000026773.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    </evidence>
    <sequence length="647" mass="70169" checksum="B92F08FE5561DFDA" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>F6YM02</accession>
    <name>F6YM02_CIOIN</name>
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        <recommendedName>
            <fullName evidence="4">diphthine methyl ester synthase</fullName>
            <ecNumber evidence="4">2.1.1.314</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100181968</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">wild type</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">S-adenosyl-L-methionine-dependent methyltransferase that catalyzes four methylations of the modified target histidine residue in translation elongation factor 2 (EF-2), to form an intermediate called diphthine methyl ester. The four successive methylation reactions represent the second step of diphthamide biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>2-[(3S)-amino-3-carboxypropyl]-L-histidyl-[translation elongation factor 2] + 4 S-adenosyl-L-methionine = diphthine methyl ester-[translation elongation factor 2] + 4 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:42652"/>
            <dbReference type="Rhea" id="RHEA-COMP:9749"/>
            <dbReference type="Rhea" id="RHEA-COMP:10173"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73995"/>
            <dbReference type="ChEBI" id="CHEBI:79005"/>
            <dbReference type="EC" id="2.1.1.314"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the diphthine synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.314" evidence="4"/>
    <dbReference type="EMBL" id="EAAA01002225">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6YM02"/>
    <dbReference type="FunCoup" id="F6YM02">
        <property type="interactions" value="428"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000018685"/>
    <dbReference type="Ensembl" id="ENSCINT00000018685.3">
        <property type="protein sequence ID" value="ENSCINP00000018685.3"/>
        <property type="gene ID" value="ENSCING00000009185.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000010568"/>
    <dbReference type="HOGENOM" id="CLU_066040_1_0_1"/>
    <dbReference type="InParanoid" id="F6YM02"/>
    <dbReference type="OMA" id="HNASIMS"/>
    <dbReference type="UniPathway" id="UPA00559"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141133">
        <property type="term" value="F:diphthine methyl ester synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd11647">
        <property type="entry name" value="DHP5_DphB"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Diphthine synthase putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1010.10:FF:000004">
        <property type="entry name" value="Putative diphthine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="entry name" value="Cobalt-precorrin-4 Transmethylase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="entry name" value="Methyltransferase, Cobalt-precorrin-4 Transmethylase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004551">
        <property type="entry name" value="Dphthn_synthase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00522">
        <property type="entry name" value="dph5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10882:SF0">
        <property type="entry name" value="DIPHTHINE METHYL ESTER SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10882">
        <property type="entry name" value="DIPHTHINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036432">
        <property type="entry name" value="Diphthine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="Tetrapyrrole methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Tetrapyrrole methylase" evidence="10">
        <location>
            <begin position="3"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
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        <location>
            <position position="85"/>
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        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
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            <position position="88"/>
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        <ligand>
            <name>S-adenosyl-L-methionine</name>
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            <position position="164"/>
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    </evidence>
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            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
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            <dbReference type="Ensembl" id="ENSCINP00000018685.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="285" mass="31790" checksum="B35520A8EDD2E703" modified="2012-04-18" version="2">MVFYLIGLGLGNPEDVTVKGLKAIKSADRVYLEAYTSILTCGKSALEEFYGRSIILADRDMVEQNSNELLRDAKKQNVVFLVVGDPFGATTHSDLVLRALEQEIPYKVIHNASIMNAVGCCGLQLYNFGETVSIVFWTDTWKPSSFCDKINENLKRGMHTLCLLDIKVKEQSIENLMRGKKVYEPPRYMTSNLACQQLLEVVEDKQSESENSVLTKETMCVSLACVGSDEQKIVAAPLNQLVNCELGPVLHSLIITGTLHPLEYDFLKICTVLYNESHSNTAKAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>F6YMT8</accession>
    <accession>A0A1W2WA86</accession>
    <name>F6YMT8_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Phosphatidylinositol glycan anchor biosynthesis class U protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100179414</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PIGU family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002124479.1">
        <property type="nucleotide sequence ID" value="XM_002124443.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6YMT8"/>
    <dbReference type="FunCoup" id="F6YMT8">
        <property type="interactions" value="224"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000028545"/>
    <dbReference type="Ensembl" id="ENSCINT00000028791.2">
        <property type="protein sequence ID" value="ENSCINP00000028545.2"/>
        <property type="gene ID" value="ENSCING00000016495.2"/>
    </dbReference>
    <dbReference type="GeneID" id="100179414"/>
    <dbReference type="KEGG" id="cin:100179414"/>
    <dbReference type="GeneTree" id="ENSGT00390000014941"/>
    <dbReference type="HOGENOM" id="CLU_030193_0_0_1"/>
    <dbReference type="InParanoid" id="F6YMT8"/>
    <dbReference type="OMA" id="ALWHLWI"/>
    <dbReference type="OrthoDB" id="549017at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042765">
        <property type="term" value="C:GPI-anchor transamidase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016255">
        <property type="term" value="P:attachment of GPI anchor to protein"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009600">
        <property type="entry name" value="PIG-U"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13121">
        <property type="entry name" value="GPI TRANSAMIDASE COMPONENT PIG-U"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13121:SF0">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL GLYCAN ANCHOR BIOSYNTHESIS CLASS U PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06728">
        <property type="entry name" value="PIG-U"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014090301" evidence="10">
        <location>
            <begin position="19"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="78"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="224"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="279"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="350"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="379"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000028545.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="431" mass="49407" checksum="3A6FA44235CE37D7" modified="2012-04-18" version="2">MTNLFVIISCGVLLRTWLLQLKDLTKWISKRPEVSTPQTSWSRLVEGFVISKQTGNVHSGDSYHGSTLLSAFLFHLQALSPLLVPAVFIACDVIAAISLHNFAKKFLQKELDDQNSHKSKLSKGVDSILLKLHHLENVPTLVALIYLFNPFTIVTCISQSSVAFNNLFLALYVAHLMSGSSIATTLFLALSTYETFYPVQLIIVAMLCQHKYQKEGNRNAAKPLFCFIFWLLILFLVSYLREGSLESIFSHYKFILTVPDQTPNIGIFWYFFTEIFNHFQTFFLFVFQINALFFFVPLSIKLRSHPICLMFILTCIISTFKSYPCVGDATLWISMLPLWSHTFKYQRQPLIVTVMLLTTTILCPVMWHMWIVAHSANANFYFAASLAYTTSHIFIMTNTVMAYLKWHYHIRKGVRLNLDGSTEKAVLRLMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>F6YWK0</accession>
    <accession>A0A1W3JQH4</accession>
    <name>F6YWK0_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Defective in cullin neddylation protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100183493</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Neddylation of cullins play an essential role in the regulation of SCF-type complexes activity.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002894">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009859664.2">
        <property type="nucleotide sequence ID" value="XM_009861362.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6YWK0"/>
    <dbReference type="FunCoup" id="F6YWK0">
        <property type="interactions" value="168"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000012594"/>
    <dbReference type="Ensembl" id="ENSCINT00000012594.3">
        <property type="protein sequence ID" value="ENSCINP00000012594.3"/>
        <property type="gene ID" value="ENSCING00000006105.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100183493"/>
    <dbReference type="KEGG" id="cin:100183493"/>
    <dbReference type="GeneTree" id="ENSGT00940000171180"/>
    <dbReference type="HOGENOM" id="CLU_047042_6_0_1"/>
    <dbReference type="InParanoid" id="F6YWK0"/>
    <dbReference type="OMA" id="SEDHGHK"/>
    <dbReference type="OrthoDB" id="286637at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097602">
        <property type="term" value="F:cullin family protein binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031624">
        <property type="term" value="F:ubiquitin conjugating enzyme binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032182">
        <property type="term" value="F:ubiquitin-like protein binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045116">
        <property type="term" value="P:protein neddylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.200:FF:000002">
        <property type="entry name" value="DCN1-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.200">
        <property type="entry name" value="Cullin, PONY binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014764">
        <property type="entry name" value="DCN-prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042460">
        <property type="entry name" value="DCN1-like_PONY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005176">
        <property type="entry name" value="PONY_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12281:SF12">
        <property type="entry name" value="DEFECTIVE IN CULLIN NEDDYLATION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12281">
        <property type="entry name" value="RP42 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03556">
        <property type="entry name" value="Cullin_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51229">
        <property type="entry name" value="DCUN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DCUN1" evidence="2">
        <location>
            <begin position="1"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU410713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000012594.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="169" mass="19741" checksum="568FA6D9F4AAF468" modified="2012-04-18" version="2">MERLCKSIKVEPGDVVMLVLAHQLNAKKMGFFTLEEWSRGMQKIQCDSSAKLERKLEVLRESLNDPVQLKSVFRYAFDFAKEPGQRTMELDTANIMLELLLSERWTLFSKFQQFLKQTKSCRVLNRDQWNNVLEFSRSILPDLSNYDFDGAWPVLIDDFVEFVKGDSTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>F6Z2M9</accession>
    <name>F6Z2M9_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">wild type</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01002782">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6ZI14"/>
    <dbReference type="STRING" id="7719.ENSCINP00000000233"/>
    <dbReference type="Ensembl" id="ENSCINT00000000233.3">
        <property type="protein sequence ID" value="ENSCINP00000000233.3"/>
        <property type="gene ID" value="ENSCING00000000128.3"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008163_0_0_1"/>
    <dbReference type="InParanoid" id="F6ZI14"/>
    <dbReference type="OMA" id="LNERLYW"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="CDD" id="cd00053">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000012">
        <property type="entry name" value="Low density lipoprotein receptor-related protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050778">
        <property type="entry name" value="Cueball_EGF_LRP_Nidogen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000033">
        <property type="entry name" value="LDLR_classB_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46513:SF13">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46513">
        <property type="entry name" value="VITELLOGENIN RECEPTOR-LIKE PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00058">
        <property type="entry name" value="Ldl_recept_b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00135">
        <property type="entry name" value="LY"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63825">
        <property type="entry name" value="YWTD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51120">
        <property type="entry name" value="LDLRB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="310"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="1">
        <location>
            <begin position="21"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="1">
        <location>
            <begin position="154"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="4">
        <location>
            <begin position="271"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="414"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS01186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000000233.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="433" mass="48626" checksum="1306075D252A9FF3" modified="2012-04-18" version="2">MTVLANQLKHAVGLTLNVKRRKVFWTDLRDERIDSVSMDNLTSVQTVLSDNEVRSPISLALDWVNDHLYWVDFSLGTVSVAYSNGSWPTTLKTMEGMSIRSVALHPEKGYMFLTAAKSRQWLIMRCSMDGNDCVDIVKENIKEPTTLAIDYSEHRLFWADAKLNMISSSDFDGSNQKIVLSVYNYLQHPFGIAICMNEVYWTDVRAHRVLKSNKWTGETTSRSSTNIYISPMEIQIDHPSTQPNMMNHCHDHPCQYMCIPLPVGGVGRYRCVCPDGYVEQGGCVKAPTTTTTTASTIATAVVGMSSGTKILIGVTVVGILVLLFFIACGALRWYQRRSLQLSMHFDNPVYRKTTTHGNEEDDHRVGIPRSGSQAHLISNEEFDDPERNEEVSVDVIVGNGRPQNTYRPLRQMSMEDTISQNSLGTSDIRNLVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>F6ZL50</accession>
    <name>F6ZL50_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Sortilin C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="F6ZL50"/>
    <dbReference type="STRING" id="7719.ENSCINP00000022444"/>
    <dbReference type="Ensembl" id="ENSCINT00000022690.2">
        <property type="protein sequence ID" value="ENSCINP00000022444.2"/>
        <property type="gene ID" value="ENSCING00000011842.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234563"/>
    <dbReference type="HOGENOM" id="CLU_951667_0_0_1"/>
    <dbReference type="InParanoid" id="F6ZL50"/>
    <dbReference type="OMA" id="EPGEYTH"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031777">
        <property type="entry name" value="Sortilin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050310">
        <property type="entry name" value="VPS10-sortilin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12106">
        <property type="entry name" value="SORTILIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12106:SF27">
        <property type="entry name" value="SORTILIN-RELATED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15901">
        <property type="entry name" value="Sortilin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003347847" description="Sortilin C-terminal domain-containing protein" evidence="1">
        <location>
            <begin position="18"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="Sortilin C-terminal" evidence="2">
        <location>
            <begin position="43"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF15901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000022444.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="278" mass="31480" checksum="34549C1749D91511" modified="2012-04-18" version="2">MIVAAQMIFLQLISVISFSNDMGRTWHKYTFTDERIHVDGLLTEPGEYTHKFTLFGHRNYRDHWKLFALDLAPLVARNCTYPDDYVNVSFARNTQNSNQCHLGMRQTFEQLKEGNCLVGSEYKRVTTTELCPCTDIDYECGYGYITTSSGCKKVVGKGSPIDDIRDILDEDTTPTIPPCVNGLRKMPTQYRKVPGDQCQNDLEGKLFTIVNCSIVVTPPPPPTIHVKLLNKTNEVKIGEVLHFTAVSNSETVWSVIRTAEPKDKITTRPVKRDEISLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>F6ZNJ4</accession>
    <name>F6ZNJ4_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Beta-1,4-galactosyltransferase</fullName>
            <ecNumber evidence="11">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC100181252</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyses the transfer of galactose onto proteins or lipids.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the glycosyltransferase 7 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="11"/>
    <dbReference type="AlphaFoldDB" id="F6ZNJ4"/>
    <dbReference type="STRING" id="7719.ENSCINP00000020931"/>
    <dbReference type="Ensembl" id="ENSCINT00000020931.3">
        <property type="protein sequence ID" value="ENSCINP00000020931.3"/>
        <property type="gene ID" value="ENSCING00000017588.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163971"/>
    <dbReference type="HOGENOM" id="CLU_044391_1_0_1"/>
    <dbReference type="InParanoid" id="F6ZNJ4"/>
    <dbReference type="OMA" id="KNESHHE"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008378">
        <property type="term" value="F:galactosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00899">
        <property type="entry name" value="b4GalT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003859">
        <property type="entry name" value="Galactosyl_T"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027791">
        <property type="entry name" value="Galactosyl_T_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027995">
        <property type="entry name" value="Galactosyl_T_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19300">
        <property type="entry name" value="BETA-1,4-GALACTOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19300:SF57">
        <property type="entry name" value="BETA-1,4-N-ACETYLGALACTOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02709">
        <property type="entry name" value="Glyco_transf_7C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13733">
        <property type="entry name" value="Glyco_transf_7N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02050">
        <property type="entry name" value="B14GALTRFASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 11" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Galactosyltransferase N-terminal" evidence="13">
        <location>
            <begin position="98"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="Galactosyltransferase C-terminal" evidence="12">
        <location>
            <begin position="231"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU368121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF13733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000020931.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="413" mass="47640" checksum="6E91C2C064D41814" modified="2012-04-18" version="2">MTIFRKLFGRTRIFSVRCKRKVGTNRSKKCLVSITVMLFIVFVYQQRAQRFVHQVKSSALLSLNTTQSTFNLRSNTIVSNAEGPPIQPTLDLKNCSNNELVGPMAINPNITPNFDEVENASSIHLLKNGCYTPTTCIPRQKVAVIIPFKERDAHLKTLLFYLHPMLQRQKISYCIFVAEQFDDGQFNKAMIMNAAFEEAMKHDWFDCIIFHDVDMIPEDDRNLYVCGEQPRHISPAIDKFDYKSHYGTEFGGVTGITPAQYRKANGHSNQFWGWGGEDNDMEFRIFNSGMKIIPSPSKIGRYKMIVHTHPWKFSANKFPTRLNKTRVARAKTDGLSDLQYRLVGSESNPLYTKWFIDIRKIEVKRITLYIDDKPAMDIDIEPGKCRWERVEGKYLDFSIELTNIKEAAPLVER</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>F6ZZC3</accession>
    <name>F6ZZC3_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Abasic site processing protein HMCES</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Embryonic stem cell-specific 5-hydroxymethylcytosine-binding protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Peptidase HMCES</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">SRAP domain-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">wild type</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SOS response-associated peptidase family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01000060">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6ZZC3"/>
    <dbReference type="FunCoup" id="F6ZZC3">
        <property type="interactions" value="49"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000012518"/>
    <dbReference type="Ensembl" id="ENSCINT00000012518.3">
        <property type="protein sequence ID" value="ENSCINP00000012518.3"/>
        <property type="gene ID" value="ENSCING00000006055.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000018439"/>
    <dbReference type="HOGENOM" id="CLU_035990_1_0_1"/>
    <dbReference type="InParanoid" id="F6ZZC3"/>
    <dbReference type="OMA" id="SYNKGPQ"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106300">
        <property type="term" value="P:protein-DNA covalent cross-linking repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1680.10">
        <property type="entry name" value="SOS response associated peptidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003738">
        <property type="entry name" value="SRAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036590">
        <property type="entry name" value="SRAP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13604:SF0">
        <property type="entry name" value="ABASIC SITE PROCESSING PROTEIN HMCES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13604">
        <property type="entry name" value="DC12-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02586">
        <property type="entry name" value="SRAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143081">
        <property type="entry name" value="BB1717-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0190">Covalent protein-DNA linkage</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="18"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="279"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000012518.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="324" mass="37140" checksum="73E06E2EA4BF8D03" modified="2012-04-18" version="2">MCGRTACTLNADDISKSSRYRDKSGKEKDPEWKLNKDKYNPSYNKAPQSYCPILINEKHLSDNVKVENNRVIVGMRWGLVPNWFTGENVNKMSFSMNNARSDTMLEKRSYNIPLRKGQRCVVLADGFYEWNTTKDGKQPYYIYFPQDLTKTAETASENVETDKKLLTMAGIFEKTFHDGEDLYSFTIITVDSHPQFSWLHHRMPAMLVNDDEIRDWLDHENIPLAKAVELIAPKDCLAWHSVSKFVNNSRNNGPQHYQCIQHEAVAKKKNEGKAMMKWLSGGATKSKTESSKPATPQVKRKASKPMDNWVKKTNRYSTAGKAKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>F6ZZZ6</accession>
    <name>F6ZZZ6_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HECT-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">ci-zf(zz)-8</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <text evidence="3">Protein modification; protein ubiquitination.</text>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Reg_chr_condens"/>
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        <source>
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        <source>
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        </source>
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    <sequence length="2337" mass="257978" checksum="045C956CF42E4B46" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>F7ABK2</accession>
    <name>F7ABK2_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">zf(bbox/ring)-4</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">wild type</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">In the C-terminal section; belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="3"/>
    <dbReference type="EMBL" id="EAAA01002082">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="EAAA01002083">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F7ABK2"/>
    <dbReference type="FunCoup" id="F7ABK2">
        <property type="interactions" value="7"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000007845"/>
    <dbReference type="Ensembl" id="ENSCINT00000007845.3">
        <property type="protein sequence ID" value="ENSCINP00000007845.3"/>
        <property type="gene ID" value="ENSCING00000003804.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158562"/>
    <dbReference type="HOGENOM" id="CLU_033905_0_0_1"/>
    <dbReference type="InParanoid" id="F7ABK2"/>
    <dbReference type="OMA" id="IQACDAK"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd19773">
        <property type="entry name" value="Bbox2_TRIM23_C-IX_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19774">
        <property type="entry name" value="Bbox2_TRIM23_C-IX_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16645">
        <property type="entry name" value="mRING-HC-C3HC3D_TRIM23_C-IX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000130">
        <property type="entry name" value="E3 ubiquitin-protein ligase TRIM23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000486">
        <property type="entry name" value="E3 ubiquitin-protein ligase TRIM23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.830.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR003649">
        <property type="entry name" value="Bbox_C"/>
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    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
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    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
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    <dbReference type="InterPro" id="IPR024156">
        <property type="entry name" value="Small_GTPase_ARF"/>
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    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
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    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
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    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
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    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
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        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
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    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
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    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11711">
        <property type="entry name" value="ADP RIBOSYLATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00328">
        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00502">
        <property type="entry name" value="BBC"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="15">
        <location>
            <begin position="32"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="16">
        <location>
            <begin position="131"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="422"/>
            <end position="429"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="429"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
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    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="446"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="468"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="524"/>
            <end position="527"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00061142"/>
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        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
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            <dbReference type="PIRSR" id="PIRSR606689-2"/>
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        <source>
            <dbReference type="PROSITE" id="PS50089"/>
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    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
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    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="585" mass="65022" checksum="6D74698296E3180F" modified="2012-04-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>F7B1W3</accession>
    <name>F7B1W3_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ribonuclease</fullName>
            <ecNumber evidence="11">3.1.26.4</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Catalytic subunit of RNase HII, an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. Participates in DNA replication, possibly by mediating the removal of lagging-strand Okazaki fragment RNA primers during DNA replication. Mediates the excision of single ribonucleotides from DNA:RNA duplexes.</text>
    </comment>
    <comment type="function">
        <text evidence="11">Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10 11">
            <text>Endonucleolytic cleavage to 5'-phosphomonoester.</text>
            <dbReference type="EC" id="3.1.26.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="10">Manganese or magnesium. Binds 1 divalent metal ion per monomer in the absence of substrate. May bind a second metal ion after substrate binding.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">The RNase H2 complex is a heterotrimer composed of the catalytic subunit RNASEH2A and the non-catalytic subunits RNASEH2B and RNASEH2C.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RNase HII family. Eukaryotic subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.4" evidence="11"/>
    <dbReference type="AlphaFoldDB" id="F7B1W3"/>
    <dbReference type="FunCoup" id="F7B1W3">
        <property type="interactions" value="304"/>
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    <dbReference type="STRING" id="7719.ENSCINP00000012778"/>
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        <property type="protein sequence ID" value="ENSCINP00000012778.3"/>
        <property type="gene ID" value="ENSCING00000006190.3"/>
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    <dbReference type="GeneTree" id="ENSGT00390000010768"/>
    <dbReference type="HOGENOM" id="CLU_036532_0_3_1"/>
    <dbReference type="InParanoid" id="F7B1W3"/>
    <dbReference type="OMA" id="REECRFF"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032299">
        <property type="term" value="C:ribonuclease H2 complex"/>
        <property type="evidence" value="ECO:0000318"/>
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    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0043137">
        <property type="term" value="P:DNA replication, removal of RNA primer"/>
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    </dbReference>
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        <property type="term" value="P:mismatch repair"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="RNase_HII_eukaryota_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.460:FF:000001">
        <property type="entry name" value="Ribonuclease"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="3.30.420.10:FF:000016">
        <property type="entry name" value="Ribonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.460">
        <property type="entry name" value="Ribonuclease hii. Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004649">
        <property type="entry name" value="RNase_H2_suA"/>
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    <dbReference type="InterPro" id="IPR001352">
        <property type="entry name" value="RNase_HII/HIII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024567">
        <property type="entry name" value="RNase_HII/HIII_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023160">
        <property type="entry name" value="RNase_HII_hlx-loop-hlx_cap_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00729">
        <property type="entry name" value="ribonuclease HII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10954">
        <property type="entry name" value="RIBONUCLEASE H2 SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10954:SF7">
        <property type="entry name" value="RIBONUCLEASE H2 SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01351">
        <property type="entry name" value="RNase_HII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51975">
        <property type="entry name" value="RNASE_H_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10 11" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="8 10 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 10 11" id="KW-0540">Nuclease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase H type-2" evidence="12">
        <location>
            <begin position="28"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000012778.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="292" mass="33001" checksum="A797EE0AC24280FE" modified="2012-04-18" version="2">MEIEHFLNNCDENFKLKSDIPKLLTSEPCCLGIDEAGRGPVLGPMVYASCFTPISCKDKLAGLGCDDSKVLKEEQRESLFETLNKASEYIGWMIHILSPAYISTSMLGRSKYNLNSISHDTAIDLIRSALKLGVEVTEVYVDTVGPPQTYAEKLKKIFPSIKFTVEKKADSKYPVVSAASICAKVCRDKAVKEWRFRENPDTDTKYGSGYPADPVTKVWLRNNVDRIFGFPSFVRFSWSTTSKLLENEAVPVHWADDDEESSNEPITSYFSKKGNSRHQFFTENKMKRVTNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F7B441</accession>
    <name>F7B441_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="F7B441"/>
    <dbReference type="Ensembl" id="ENSCINT00000017024.3">
        <property type="protein sequence ID" value="ENSCINP00000017024.3"/>
        <property type="gene ID" value="ENSCING00000008344.3"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1585885_0_0_1"/>
    <dbReference type="InParanoid" id="F7B441"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="75"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000017024.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="168" mass="19781" checksum="08498097BD3FF9DC" modified="2012-04-18" version="2">MWDWKCVRQNCTEVRRNLESNFSTEDTLIVARRLARRMSEYRTKPIIINKSLLNASHRNKVFNNYPSVSPSLKQNRLSIQPTEERRLSSSDEDIKVNEETNKKSHDRRDTKHDIEPQTISNKLHGLLVTLKQIEKDFNRHHSAALSQLQNFERSLREIGLIIDDTTEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>F7B4S3</accession>
    <name>F7B4S3_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Chapman J."/>
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                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
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                <person name="Martinez D."/>
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                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
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                <person name="Lee B.I."/>
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                <person name="Manohar C."/>
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                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
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                <person name="Nishizaka S."/>
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                <person name="Ohta F."/>
                <person name="Oishi K."/>
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                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
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                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
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                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4">wild type</strain>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
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                <person name="Richardson P."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
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                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="11">wild type</strain>
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        <scope>IDENTIFICATION</scope>
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <name type="scientific">Ciona intestinalis</name>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">wild type</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome 12"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="ZP_dom"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14002:SF20">
        <property type="entry name" value="ZONA PELLUCIDA-LIKE DOMAIN-CONTAINING PROTEIN 1"/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Hastings K.E."/>
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                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
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                <person name="Meinertzhagen I.A."/>
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                <person name="Terry A."/>
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                <person name="Azumi K."/>
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                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
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                <person name="Richardson P."/>
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    <reference evidence="8" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
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                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
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                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">wild type</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="8" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <comment type="similarity">
        <text evidence="2 6">Belongs to the cytochrome P450 family.</text>
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        <property type="component" value="Chromosome 11"/>
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    <dbReference type="GO" id="GO:0005737">
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        <property type="evidence" value="ECO:0000318"/>
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        <property type="term" value="F:heme binding"/>
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        <property type="term" value="F:iron ion binding"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen"/>
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        <property type="entry name" value="Cytochrome P450"/>
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    <dbReference type="InterPro" id="IPR050182">
        <property type="entry name" value="Cytochrome_P450_fam2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300:SF397">
        <property type="entry name" value="CYTOCHROME P450 2U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300">
        <property type="entry name" value="CYTOCHROME P450 508A4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0349">Heme</keyword>
    <keyword evidence="4 5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="206"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="5">
        <location>
            <position position="444"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000030185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="498" mass="57118" checksum="75A779BE1D1228DE" modified="2012-04-18" version="1">VWNALFVILFDCPANTITLSIIVFYVTWHYWNKRTPGSPPGPRGFPFIGAITSIRKHPEHVMTKWNKEYGPVCMVRLGFKDVLVIGSYEAAHEAYVKSQDFLDRPSPFGLEILGGGYGLFPIAYGSFHQEQRRFGLNTLREHGMGRRVLESTILQYAEELCDRLETKMSAPVLLDQEVYIAVSSTIAHIVFGHNTMQDSPEFRDMILWMLRPNTATVLAGILVFAPYLKHLPFFRGVHNDSRALRLKLESLNLKEVKKHDKTRDPSDPRDFIDSFLNEMDKRKRKNSKQATGKVFILGKVRDLFLAGTDTTSATTCWIILFLCKYPDVQRRMQKEIDDVIGENGIPKLALAERLPFTRAVIQEMGRIRPNVPLAVPHCASRDSTLMGFNIPKDTIIMTNIWGIHHDEKTWKDPYKFNPDRHLDAEGNFVKSNHVMQFNIGLRSCLGQQLARMELFLITVTLFRKFFIELEPGCDIDMEGESLVSLRPYPFKVLLTKRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>H2XKN4</accession>
    <name>H2XKN4_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SAM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="H2XKN4"/>
    <dbReference type="STRING" id="7719.ENSCINP00000030216"/>
    <dbReference type="Ensembl" id="ENSCINT00000034256.1">
        <property type="protein sequence ID" value="ENSCINP00000030216.1"/>
        <property type="gene ID" value="ENSCING00000022196.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156403"/>
    <dbReference type="HOGENOM" id="CLU_2454032_0_0_1"/>
    <dbReference type="InParanoid" id="H2XKN4"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09540">
        <property type="entry name" value="SAM_EPS8-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039801">
        <property type="entry name" value="EPS8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041418">
        <property type="entry name" value="SAM_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12287:SF23">
        <property type="entry name" value="AROUSER, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12287">
        <property type="entry name" value="EPIDERMAL GROWTH FACTOR RECEPTOR KINASE SUBSTRATE EPS8-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18016">
        <property type="entry name" value="SAM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAM" evidence="2">
        <location>
            <begin position="2"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000030216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="89" mass="9912" checksum="4115EDE751FCA4A1" modified="2012-04-18" version="1">MVLTKDSSQAEVKTWLLSHGFSSLTIDSLSVLNGAQIFSLTKDELKMICDDEGSRVHSQLLVQKSMPSSATNGVSELDKIMERRRRELS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>H2XKN9</accession>
    <accession>A0A1W2WNW1</accession>
    <name>H2XKN9_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phospholipid scramblase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC101242405</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">May mediate accelerated ATP-independent bidirectional transbilayer migration of phospholipids upon binding calcium ions that results in a loss of phospholipid asymmetry in the plasma membrane.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the phospholipid scramblase family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002524">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_004226230.1">
        <property type="nucleotide sequence ID" value="XM_004226182.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XKN9"/>
    <dbReference type="Ensembl" id="ENSCINT00000036294.1">
        <property type="protein sequence ID" value="ENSCINP00000030221.1"/>
        <property type="gene ID" value="ENSCING00000022559.1"/>
    </dbReference>
    <dbReference type="GeneID" id="101242405"/>
    <dbReference type="KEGG" id="cin:101242405"/>
    <dbReference type="GeneTree" id="ENSGT00940000166623"/>
    <dbReference type="HOGENOM" id="CLU_1325979_0_0_1"/>
    <dbReference type="InParanoid" id="H2XKN9"/>
    <dbReference type="OMA" id="VEDSHCC"/>
    <dbReference type="OrthoDB" id="191150at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017128">
        <property type="term" value="F:phospholipid scramblase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017121">
        <property type="term" value="P:plasma membrane phospholipid scrambling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005552">
        <property type="entry name" value="Scramblase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23248:SF9">
        <property type="entry name" value="PHOSPHOLIPID SCRAMBLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23248">
        <property type="entry name" value="PHOSPHOLIPID SCRAMBLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03803">
        <property type="entry name" value="Scramblase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="2" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0564">Palmitate</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU363116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000030221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="207" mass="23438" checksum="08C4D6C7C0BC0AFA" modified="2012-04-18" version="1">MSLAVTSQPHAIVREKHLSENVWIRKPKPGQHVLEKMSDLSDARHLHVHRNVKGTICSGKQDYDITDQDGKLMYKAVEDSHCCCVHCCGPARAYKIIVKDPLGLELLQFHRPVRCSDTCLCCCGCGLMRMRVTEACSDVVIGDVKQRCTFIREKFDVTELVENEQTTLIGPSVTCRCFANMTFQIIDDKNSEVGEIWKRWKGAPNEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>H2XLX6</accession>
    <name>H2XLX6_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CUB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">wild type</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002294">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XLX6"/>
    <dbReference type="Ensembl" id="ENSCINT00000033835.1">
        <property type="protein sequence ID" value="ENSCINP00000030658.1"/>
        <property type="gene ID" value="ENSCING00000023599.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000171949"/>
    <dbReference type="HOGENOM" id="CLU_015228_5_2_1"/>
    <dbReference type="InParanoid" id="H2XLX6"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24251:SF37">
        <property type="entry name" value="CUB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24251">
        <property type="entry name" value="OVOCHYMASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003577992" description="CUB domain-containing protein" evidence="4">
        <location>
            <begin position="24"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="5">
        <location>
            <begin position="27"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="5">
        <location>
            <begin position="149"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000030658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="196" mass="21764" checksum="DD31B219FE6BAF92" modified="2012-04-18" version="1">MILRGCIIWLFGLFSVLFNQGLAQTACGFNARATTEAQTIRSPATADGRYRNNENCVWTLTASPGLLVNLKIIQLRTESCCDYLQVTLSQQTILLRGHRSNVTFRGQRIRLQFITDGSFRRQGFTATYRAVSENAVLEPTTTLAPGAPCGFNANATSELQPLNSPGYPHNYGNDANCTWTITAQDGFLVQFTFYDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>H2XMT0</accession>
    <name>H2XMT0_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="H2XMT0"/>
    <dbReference type="Ensembl" id="ENSCINT00000034709.1">
        <property type="protein sequence ID" value="ENSCINP00000030963.1"/>
        <property type="gene ID" value="ENSCING00000020858.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00660000097152"/>
    <dbReference type="HOGENOM" id="CLU_1880040_0_0_1"/>
    <dbReference type="InParanoid" id="H2XMT0"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="4"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000030963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="136" mass="15570" checksum="0CD10493C9FEE695" modified="2012-04-18" version="1">MKQLESTELQASTLSAMVAELEAARDVHVGEYKQLDDQLTKVRSDFKTEVATLEERNKSLEQKLEASSDDMRVKDEYIVCEKREAEELRARLSVAVANIEELEGFKGQFSEIENRHKNLVSEYECMEVELLRVGGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>H2XPD8</accession>
    <name>H2XPD8_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Abnormal spindle-like microcephaly-associated protein ASH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
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                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
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                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
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            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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    <reference evidence="5" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
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                <consortium name="Ensembl"/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Ho I."/>
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                <person name="Huang W."/>
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                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="34" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="34">wild type</strain>
        </source>
    </reference>
    <reference evidence="34" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="34" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Preferential cleavage of polypeptides between hydrophobic residues, particularly with Phe or Tyr at P1'.</text>
            <dbReference type="EC" id="3.4.24.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>neurotensin + H2O = neurotensin(1-10) + L-tyrosyl-L-isoleucyl-L-leucine</text>
            <dbReference type="Rhea" id="RHEA:71479"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:147362"/>
            <dbReference type="ChEBI" id="CHEBI:190705"/>
            <dbReference type="ChEBI" id="CHEBI:190707"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:71480"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>neurotensin + H2O = neurotensin(1-11) + L-isoleucyl-L-leucine</text>
            <dbReference type="Rhea" id="RHEA:71475"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:147362"/>
            <dbReference type="ChEBI" id="CHEBI:190704"/>
            <dbReference type="ChEBI" id="CHEBI:190706"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="28">
            <dbReference type="Rhea" id="RHEA:71476"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>substance P + H2O = substance P(1-7) + L-Phe-Gly-L-Leu-L-Met-NH2</text>
            <dbReference type="Rhea" id="RHEA:71467"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:190692"/>
            <dbReference type="ChEBI" id="CHEBI:190695"/>
            <dbReference type="ChEBI" id="CHEBI:190698"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="27">
            <dbReference type="Rhea" id="RHEA:71468"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>substance P + H2O = substance P(1-9) + L-Leu-L-Met-NH2</text>
            <dbReference type="Rhea" id="RHEA:71459"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:190692"/>
            <dbReference type="ChEBI" id="CHEBI:190693"/>
            <dbReference type="ChEBI" id="CHEBI:190700"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="29">
            <dbReference type="Rhea" id="RHEA:71460"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase M13 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.11" evidence="5"/>
    <dbReference type="EMBL" id="EAAA01000382">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XQ28"/>
    <dbReference type="STRING" id="7719.ENSCINP00000031762"/>
    <dbReference type="Ensembl" id="ENSCINT00000034380.1">
        <property type="protein sequence ID" value="ENSCINP00000031762.1"/>
        <property type="gene ID" value="ENSCING00000017942.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164877"/>
    <dbReference type="HOGENOM" id="CLU_006187_8_0_1"/>
    <dbReference type="InParanoid" id="H2XQ28"/>
    <dbReference type="OMA" id="FARAYHC"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd08662">
        <property type="entry name" value="M13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1380.10">
        <property type="entry name" value="Neutral endopeptidase , domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000718">
        <property type="entry name" value="Peptidase_M13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018497">
        <property type="entry name" value="Peptidase_M13_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042089">
        <property type="entry name" value="Peptidase_M13_dom_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008753">
        <property type="entry name" value="Peptidase_M13_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11733:SF114">
        <property type="entry name" value="NEPRILYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11733">
        <property type="entry name" value="ZINC METALLOPROTEASE FAMILY M13 NEPRILYSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01431">
        <property type="entry name" value="Peptidase_M13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05649">
        <property type="entry name" value="Peptidase_M13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00786">
        <property type="entry name" value="NEPRILYSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51885">
        <property type="entry name" value="NEPRILYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="20" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="21" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="18" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="11" id="KW-0519">Myristate</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-0645">Protease</keyword>
    <keyword evidence="35" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="31" id="KW-0732">Signal</keyword>
    <keyword evidence="15" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="14" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="31">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003576921" description="Neprilysin" evidence="31">
        <location>
            <begin position="20"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M13 N-terminal" evidence="33">
        <location>
            <begin position="64"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M13 C-terminal" evidence="32">
        <location>
            <begin position="520"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="30">
        <location>
            <begin position="370"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="30">
        <location>
            <begin position="370"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00031127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00031362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00031486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00032584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00047638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00048093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00049273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00049470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="32">
        <source>
            <dbReference type="Pfam" id="PF01431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="33">
        <source>
            <dbReference type="Pfam" id="PF05649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000031762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="35">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="732" mass="80910" checksum="589EE84C8560BE40" modified="2012-04-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>H2XQ41</accession>
    <name>H2XQ41_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">G/T mismatch-specific thymine DNA glycosylase</fullName>
            <ecNumber evidence="15">3.2.2.29</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Thymine-DNA glycosylase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>Hydrolyzes mismatched double-stranded DNA and polynucleotides, releasing free thymine.</text>
            <dbReference type="EC" id="3.2.2.29"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="14">Homodimer. Interacts with AICDA and GADD45A.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the uracil-DNA glycosylase (UDG) superfamily. TDG/mug family.</text>
    </comment>
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        <property type="gene ID" value="ENSCING00000020650.1"/>
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    <dbReference type="GeneTree" id="ENSGT00390000000987"/>
    <dbReference type="HOGENOM" id="CLU_042829_2_0_1"/>
    <dbReference type="InParanoid" id="H2XQ41"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="evidence" value="ECO:0000318"/>
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        <property type="term" value="F:G/T mismatch-specific thymine-DNA glycosylase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="term" value="F:pyrimidine-specific mismatch base pair DNA N-glycosylase activity"/>
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        <property type="term" value="F:SUMO binding"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="F:uracil DNA N-glycosylase activity"/>
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        <property type="term" value="P:epigenetic regulation of gene expression"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="InterPro" id="IPR036895">
        <property type="entry name" value="Uracil-DNA_glycosylase-like_sf"/>
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        <property type="entry name" value="G_T MISMATCH-SPECIFIC THYMINE DNA GLYCOSYLASE"/>
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    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0832">Ubl conjugation</keyword>
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        <location>
            <begin position="84"/>
            <end position="234"/>
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        <location>
            <begin position="26"/>
            <end position="51"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
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            <dbReference type="ARBA" id="ARBA00022801"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022843"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00052915"/>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00064519"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF03167"/>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000031775.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    </evidence>
    <sequence length="250" mass="28413" checksum="4658315C922974E0" modified="2012-04-18" version="1">MFLWNFIENLRNIVNRIEMATLQNGSSPVVKKRGRPPKIKPESGATPSKQTKITDNMKKTKRTVDRFKGVTKEELMTRLLPDRIKHGLDILIIGINPGLFAAYKGHHYAGPGNHFWKCINLSGMVGKPVTSDNDVELMEYGIGFSTIVQRTTPSAKDLTSKEIREGGKELIEKIKIYQPKIAVFNGKGIYEIFSKEMFGKKKKDFLFGRQAERIPGTNTHIFVMPSSSARCAQFPRAQDKVHYYIQIKRL</sequence>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
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        <name type="scientific">Ciona intestinalis</name>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="H2XQE0"/>
    <dbReference type="Ensembl" id="ENSCINT00000033421.1">
        <property type="protein sequence ID" value="ENSCINP00000031874.1"/>
        <property type="gene ID" value="ENSCING00000023422.1"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2385491_0_0_1"/>
    <dbReference type="InParanoid" id="H2XQE0"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="15"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000031874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="94" mass="10221" checksum="A7FC4073183F5C75" modified="2012-04-18" version="1">MNYYKLLSSPCKPPSADGTSSSSKISDTNISAFNSTMPPCDGLQSNQTQTFPRSIRNDGSILKYQHENRLLEPAGGTEPLDVSHVNSLFKKSSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>H2XR08</accession>
    <name>H2XR08_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Mannosyltransferase</fullName>
            <ecNumber evidence="10">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
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            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
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                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
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                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
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            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">wild type</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <text evidence="1">Belongs to the unc-93 family.</text>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Yamada L."/>
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                <person name="Wiley G.B."/>
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                <person name="Roe B.A."/>
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                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1">wild type</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
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        <source>
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    <sequence length="29" mass="3489" checksum="0EDF355422B2363E" modified="2012-04-18" version="1">MKGLSTYPRSHRFFSKIAYFNISCFINFI</sequence>
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    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
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                <person name="Hastings K.E."/>
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                <person name="Kawashima T."/>
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                <person name="Meinertzhagen I.A."/>
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                <person name="Putnam N."/>
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                <person name="Wang H.G."/>
                <person name="Awazu S."/>
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                <person name="Hayashi H."/>
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                <person name="Makabe K.W."/>
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        <property type="component" value="Chromosome 2"/>
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        <property type="term" value="C:endoplasmic reticulum"/>
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        <property type="term" value="F:phosphatidylcholine lysophospholipase activity"/>
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        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="cNMP-bd_dom"/>
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    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
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        <property type="entry name" value="cAMP-binding domain-like"/>
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        <property type="entry name" value="CNMP_BINDING_3"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="2">
        <location>
            <begin position="175"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000034100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="225" mass="26242" checksum="07EFEFC8C7CAAB36" modified="2012-04-18" version="1">MDYFGAVNNWLDLFNMSSSSSEESSMVVMWVGAIATLLLVFLTFAYFFKTKMQVPVISIPEVESNKKYRFRKRDKVQFYARKVFRKGRDTASKLQKQVSMIKEGVSVPEYSRSGRRLDNRERMMILAKRMFSGKKGPSLLLKKELPSLLLEADQEEEQFPNLPEEVMYLLQSVKVFGHFEKGVFLQLCRHMETINLAQGDYLFKVGEEDKWLYVVQEGQVNLFIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>H2XX64</accession>
    <name>H2XX64_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">protein phosphatase methylesterase-1</fullName>
            <ecNumber evidence="3">3.1.1.89</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">wild type</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Demethylates proteins that have been reversibly carboxymethylated. Demethylates PPP2CB (in vitro) and PPP2CA. Binding to PPP2CA displaces the manganese ion and inactivates the enzyme.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>[phosphatase 2A protein]-C-terminal L-leucine methyl ester + H2O = [phosphatase 2A protein]-C-terminal L-leucine + methanol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48548"/>
            <dbReference type="Rhea" id="RHEA-COMP:12134"/>
            <dbReference type="Rhea" id="RHEA-COMP:12135"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17790"/>
            <dbReference type="ChEBI" id="CHEBI:90516"/>
            <dbReference type="ChEBI" id="CHEBI:90517"/>
            <dbReference type="EC" id="3.1.1.89"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Binds PPP2CA and PPP2CB.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.89" evidence="3"/>
    <dbReference type="EMBL" id="EAAA01001521">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XX64"/>
    <dbReference type="FunCoup" id="H2XX64">
        <property type="interactions" value="966"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000034248"/>
    <dbReference type="Ensembl" id="ENSCINT00000036500.1">
        <property type="protein sequence ID" value="ENSCINP00000034248.1"/>
        <property type="gene ID" value="ENSCING00000021012.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000004396"/>
    <dbReference type="HOGENOM" id="CLU_024818_0_2_1"/>
    <dbReference type="InParanoid" id="H2XX64"/>
    <dbReference type="OMA" id="MHARISM"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051723">
        <property type="term" value="F:protein methylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
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        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016812">
        <property type="entry name" value="PPase_methylesterase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14189:SF0">
        <property type="entry name" value="PROTEIN PHOSPHATASE METHYLESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14189">
        <property type="entry name" value="PROTEIN PHOSPHATASE METHYLESTERASE-1 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
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    <feature type="domain" description="AB hydrolase-1" evidence="8">
        <location>
            <begin position="71"/>
            <end position="182"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00024698"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00049203"/>
        </source>
    </evidence>
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            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000034248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="228" mass="25055" checksum="9E24BA59335722D8" modified="2012-04-18" version="1">MSSLRQNVLKQGLPPLPAQTSQPPSANRFSVGRKKDYSVLPWTNYFEEVKDVKSGGNTFRVYLKGNTGTRIVFLHGGGFSGLSWAVLSKCLTNLIECQCIAPDLRGHGSSVTEDDSNLSSEQLANDVCDIIEEMNEDCSPVVLVGHSMGGAIAVHTAMQNRLRSLAALIMIDVVEGTAMASLHMMHQILRNRPQDFESDEKAIEWCVRSGYIRNLESARASMIGQLKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>H2XXM8</accession>
    <name>H2XXM8_CIOIN</name>
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        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
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            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="H2XXM8"/>
    <dbReference type="STRING" id="7719.ENSCINP00000034412"/>
    <dbReference type="Ensembl" id="ENSCINT00000031933.1">
        <property type="protein sequence ID" value="ENSCINP00000034412.1"/>
        <property type="gene ID" value="ENSCING00000019804.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000169900"/>
    <dbReference type="HOGENOM" id="CLU_1437470_0_0_1"/>
    <dbReference type="InParanoid" id="H2XXM8"/>
    <dbReference type="OMA" id="FNNNGRV"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052065">
        <property type="entry name" value="Compl_asym_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22906">
        <property type="entry name" value="PROPERDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22906:SF43">
        <property type="entry name" value="PROPERDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00090">
        <property type="entry name" value="TSP_1"/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
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                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
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                <person name="Huang W."/>
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                <person name="Meinertzhagen I.A."/>
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                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
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    <dbReference type="AlphaFoldDB" id="H2XZ36"/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
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                <person name="Huang W."/>
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                <person name="Lemaire P."/>
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                <person name="Meinertzhagen I.A."/>
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                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
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                <person name="Kobayashi K."/>
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                <person name="Nomoto H."/>
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                <person name="Oishi K."/>
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                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">wild type</strain>
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    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="ENSCING00000023483.1"/>
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        <property type="component" value="Chromosome 14"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000034988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="118" mass="13060" checksum="98C2E3CF75C86CFF" modified="2012-04-18" version="1">MICRVMSKRYVDWVMVTETHEMFTHAIELEIENVVENKNVRAQCIALSEKTLSAKFDEAKARSSLWNKDGSPAHSVGVFGKIGKKLGLFAGKSPTKLLYLVKDLKSIAHLKALGLGGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>H2XZM2</accession>
    <name>H2XZM2_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <source>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
    <sequence length="246" mass="27494" checksum="888B35E37CB75E25" modified="2012-04-18" version="1">MNMNVHRQIGRMSSQPIDPPPPPPCKPQTTENHEGNYEEPVFFKGNSPRSRLDSYNKPLPPLPNENKPLPPTPEESQENYIDVAGNVDNYDIVQGEYQQLESDDSYENNPISSPVNQTSPVNQYRPPHLHNKPPTPGAKPNMMQQLKQTLEGNKHPPPPPGLKPTSNTFDHKPPTSPINRPKLKTPKPSPKKSHPMPTLQAAYEEPPQVQPAYVDTSVIQAEYLDTSQFEPTQTHNPPMPPPKPAS</sequence>
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    <accession>H2XZV3</accession>
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        <recommendedName>
            <fullName evidence="6">Peptidase S1 domain-containing protein</fullName>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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            <taxon>Phlebobranchia</taxon>
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    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the peptidase S1 family. CLIP subfamily.</text>
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    <dbReference type="MEROPS" id="S01.152"/>
    <dbReference type="Ensembl" id="ENSCINT00000032039.1">
        <property type="protein sequence ID" value="ENSCINP00000035187.1"/>
        <property type="gene ID" value="ENSCING00000022850.1"/>
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        <property type="term" value="F:serine-type endopeptidase activity"/>
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        <property type="project" value="InterPro"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Transmembrane protease serine"/>
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        <property type="entry name" value="Trypsin-like serine proteases"/>
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        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050127">
        <property type="entry name" value="Serine_Proteases_S1"/>
    </dbReference>
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        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
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        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24264:SF54">
        <property type="entry name" value="PEPTIDASE S1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24264">
        <property type="entry name" value="TRYPSIN-RELATED"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Trypsin"/>
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        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
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    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="Peptidase S1" evidence="6">
        <location>
            <begin position="1"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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    </evidence>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">wild type</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <source>
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    </evidence>
    <sequence length="200" mass="24526" checksum="D4869E3664D03257" modified="2012-04-18" version="1">MKLHAWFIQWREMHGKAVVADLHVRRVQCNRYFTTWVKYATSRKSQATNVKLIVHRKSYQQLRSAWKRWKEELQRKKNDYRMLKMILDARIGRYFIIWRKNLDLHFATKDYQRRVTISTWKLWMEATNNKQVAIQHYEEKMELLLKKVLNEWHIMTSHNQLLRNNQQCLSSNINNNLVSGCFRIWKREHDNLMIAKSYDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>H2Y0Q2</accession>
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        <name type="scientific">Ciona intestinalis</name>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
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                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
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                <person name="Levine M."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">wild type</strain>
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    <reference evidence="6" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="6" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <text evidence="4">Belongs to the ubiquitin-conjugating enzyme family.</text>
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        <source>
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    </evidence>
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        <source>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
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                <person name="Meinertzhagen I.A."/>
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                <person name="Rash S."/>
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                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
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                <person name="Makabe K.W."/>
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                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
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                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
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                <person name="Richardson P."/>
                <person name="Lucas S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
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                <person name="Sasakura Y."/>
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                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">wild type</strain>
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    <reference evidence="1" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000035873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="58" mass="6727" checksum="1ABAD6C5A86B1F80" modified="2012-04-18" version="1">MIPVSCVGVQSHIFDFCKKCDFFEFWYKHMIPVSCVGVQSHIFDFCKKCDFLSIGINT</sequence>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
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    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
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                <person name="Doyle S."/>
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                <person name="Kobayashi M."/>
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                <person name="Wiley G.B."/>
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                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Hastings K.E."/>
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        <source>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
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                <person name="Huang W."/>
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                <person name="Martinez D."/>
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                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
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                <person name="Hayashi H."/>
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                <person name="Kobayashi K."/>
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                <person name="Mount S."/>
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                <person name="Sasaki A."/>
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                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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            </authorList>
            <dbReference type="PubMed" id="12481130"/>
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        <source>
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        <source>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
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                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
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                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
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                <person name="Zhang C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="9" key="2">
        <citation type="journal article" date="2006" name="Dev. Biol." volume="292" first="546" last="554">
            <title>Systematic analysis of embryonic expression profiles of zinc finger genes in Ciona intestinalis.</title>
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                <person name="Chiba T."/>
                <person name="Horii R."/>
                <person name="Yamada L."/>
                <person name="Kubo A."/>
                <person name="Miyamura D."/>
                <person name="Satoh N."/>
                <person name="Satou Y."/>
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            <dbReference type="DOI" id="10.1016/j.ydbio.2006.01.024"/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="entry name" value="WD40_rpt"/>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2006" name="Dev. Biol." volume="292" first="546" last="554">
            <title>Systematic analysis of embryonic expression profiles of zinc finger genes in Ciona intestinalis.</title>
            <authorList>
                <person name="Miwata K."/>
                <person name="Chiba T."/>
                <person name="Horii R."/>
                <person name="Yamada L."/>
                <person name="Kubo A."/>
                <person name="Miyamura D."/>
                <person name="Satoh N."/>
                <person name="Satou Y."/>
            </authorList>
            <dbReference type="PubMed" id="16519883"/>
            <dbReference type="DOI" id="10.1016/j.ydbio.2006.01.024"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Core component of RNA polymerase III (Pol III) which synthesizes small non-coding RNAs using the four ribonucleoside triphosphates as substrates. Can mediate Pol I proofreading of the nascent RNA transcript. Anchors into the Pol III active site to constantly monitor transcription fidelity, cleaves mis-incorporated 5'-ribonucleotides and restarts the transcription process. Once Pol III reaches the poly(dT) termination signal, can induce Pol III clamp opening and transcription termination. Pol III plays an important role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as a nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF-kappa-B through the RIG-I pathway.</text>
    </comment>
    <comment type="function">
        <text evidence="10">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the RNA polymerase III complex consisting of 17 subunits: a ten-subunit horseshoe-shaped catalytic core composed of POLR3A/RPC1, POLR3B/RPC2, POLR1C/RPAC1, POLR1D/RPAC2, POLR3K/RPC10, POLR2E/RPABC1, POLR2F/RPABC2, POLR2H/RPABC3, POLR2K/RPABC4 and POLR2L/RPABC5; a mobile stalk composed of two subunits POLR3H/RPC8 and CRCP/RPC9, protruding from the core and functioning primarily in transcription initiation; and additional subunits homologous to general transcription factors of the RNA polymerase II machinery, POLR3C/RPC3-POLR3F/RPC6-POLR3G/RPC7 heterotrimer required for transcription initiation and POLR3D/RPC4-POLR3E/RPC5 heterodimer involved in both transcription initiation and termination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10 14">Belongs to the archaeal rpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family.</text>
    </comment>
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        <property type="protein sequence ID" value="BAE93339.1"/>
        <property type="molecule type" value="mRNA"/>
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    <dbReference type="RefSeq" id="NP_001037832.1">
        <property type="nucleotide sequence ID" value="NM_001044367.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1RPU6"/>
    <dbReference type="STRING" id="7719.ENSCINP00000030689"/>
    <dbReference type="Ensembl" id="ENSCINT00000035229.1">
        <property type="protein sequence ID" value="ENSCINP00000030689.1"/>
        <property type="gene ID" value="ENSCING00000018769.1"/>
    </dbReference>
    <dbReference type="GeneID" id="751592"/>
    <dbReference type="KEGG" id="cin:751592"/>
    <dbReference type="CTD" id="51728"/>
    <dbReference type="GeneTree" id="ENSGT00550000075071"/>
    <dbReference type="HOGENOM" id="CLU_093932_3_0_1"/>
    <dbReference type="OMA" id="MEFCDEC"/>
    <dbReference type="OrthoDB" id="282152at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061484">
        <property type="term" value="P:hematopoietic stem cell homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006386">
        <property type="term" value="P:termination of RNA polymerase III transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd10509">
        <property type="entry name" value="Zn-ribbon_RPC11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.10:FF:000005">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019761">
        <property type="entry name" value="DNA-dir_RNA_pol-M_15_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012164">
        <property type="entry name" value="Rpa12/Rpb9/Rpc10/TFS"/>
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    <dbReference type="InterPro" id="IPR001529">
        <property type="entry name" value="Zn_ribbon_RPB9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034014">
        <property type="entry name" value="Zn_ribbon_RPC11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001222">
        <property type="entry name" value="Znf_TFIIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11239">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11239:SF12">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02150">
        <property type="entry name" value="Zn_ribbon_RPB9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01096">
        <property type="entry name" value="Zn_ribbon_TFIIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005586">
        <property type="entry name" value="RNApol_RpoM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00661">
        <property type="entry name" value="RPOL9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00440">
        <property type="entry name" value="ZnF_C2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01030">
        <property type="entry name" value="RNA_POL_M_15KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00466">
        <property type="entry name" value="ZF_TFIIS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51133">
        <property type="entry name" value="ZF_TFIIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="2 10 14" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="3 11 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10 14" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 12 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIS-type" evidence="15">
        <location>
            <begin position="65"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="12">
        <location>
            <begin position="5"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="5"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044007"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00054653"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRNR" id="PIRNR005586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR005586-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR005586-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003474"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51133"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BAE93339.1"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="108" mass="12321" checksum="289BB61615CF5BE1" modified="2006-05-16" version="1">MLLFCPTCGNLLTVEEGNQCYRFACPTCPYVHNITQKISSKKFPKLKAVDDVLGGSAAWENVDSTEEKCPKCEHDRAYFMQIQTRSADEPMTIFYKCCSPTCGHQWRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-08-30" modified="2026-01-28" version="158" xmlns="https://uniprot.org/uniprot">
    <accession>Q4H3D5</accession>
    <accession>A0A1W2VP20</accession>
    <name>Q4H3D5_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="14 15">Nuclear receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">Ci-HNF4</name>
        <name evidence="15" type="synonym">hnf4</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
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        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2005-04" db="EMBL/GenBank/DDBJ databases">
            <title>Expressed genes in Ciona intestinalis.</title>
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                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
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                <person name="Hastings K.E."/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
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                <person name="Oishi K."/>
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                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51019">
        <property type="entry name" value="REELIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014069063" evidence="1">
        <location>
            <begin position="20"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="Reelin" evidence="2">
        <location>
            <begin position="14"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000023876.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="203" mass="21785" checksum="B349085275CAEB98" modified="2017-06-07" version="1">MVAMLYWFLWIYLATLTSAYPGGAPDGSCGHMTPAHGSPASNLDQSPFYLNVSATSYLPGEKIIVTVLSSGANFKGMFLQARGEDSPNNAIGWWEKPNGTSFKQMECAGNKNSALTHSDGMSKAAPSRFVWIAPCNSTTDYRFYATIVQEYATFYVKIPSDTVVRGNGEKVCKPPGSGGTGVSLPPLSMTLFVAIVHAVALLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-06-07" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1W2WK21</accession>
    <accession>F7B089</accession>
    <name>A0A1W2WK21_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Phorbol-ester/DAG-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">wild type</strain>
        </source>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <location evidence="1">Sarcolemma</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Endomembrane system</location>
            <topology evidence="12">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the synaptobrevin family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002017">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1W2WK21"/>
    <dbReference type="STRING" id="7719.ENSCINP00000017996"/>
    <dbReference type="Ensembl" id="ENSCINT00000017996.3">
        <property type="protein sequence ID" value="ENSCINP00000017996.3"/>
        <property type="gene ID" value="ENSCING00000008839.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000170580"/>
    <dbReference type="InParanoid" id="A0A1W2WK21"/>
    <dbReference type="OMA" id="CKRCEVD"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042383">
        <property type="term" value="C:sarcolemma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20793">
        <property type="entry name" value="C1_cPKC_nPKC_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000022">
        <property type="entry name" value="SH3 and cysteine-rich domain-containing protein 3 isoform 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020454">
        <property type="entry name" value="DAG/PE-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001388">
        <property type="entry name" value="Synaptobrevin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042855">
        <property type="entry name" value="V_SNARE_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22968">
        <property type="entry name" value="PROTEIN KINASE C, MU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22968:SF24">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00957">
        <property type="entry name" value="Synaptobrevin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00008">
        <property type="entry name" value="DAGPEDOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00219">
        <property type="entry name" value="SYNAPTOBREVN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50892">
        <property type="entry name" value="V_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13 14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="9" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="284"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="17">
        <location>
            <begin position="6"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="17">
        <location>
            <begin position="63"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="V-SNARE coiled-coil homology" evidence="18">
        <location>
            <begin position="220"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="130"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="232"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="134"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="159"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008025"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00046280"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000017996.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="21">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="312" mass="34982" checksum="FBF111271A987115" modified="2017-06-07" version="1">MQSISQHMFQLHNYKRPTLCAHCGGLLVGLRKQGLRCKRCEVDVHEKCQEDLSKACIEVIVGQHNFREKTYKQPTFCDFCGKLLVGVVNQGIKCSECGTNVHEKCQDKIEVHCSETKSRKNKEAIHSTGISRVKGSRRTVRPRGKKANEKTPLQSMDQLAEEADEEREADMSSMFKTMPAKSPRHSLTATPVHSKPASPAGSPKMPRSPAKGASTSGGEKIGEMNKMVGDVVERMKENADRLAEREEKLDTLKNNAEELEEKAKIFDKKASDLKRKFWYQNYKLWIILGVAVTILILVIVIAVVFAGKKKTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-06-07" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1W3JJA3</accession>
    <accession>F6PMQ2</accession>
    <name>A0A1W3JJA3_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized LOC100178806</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100178806</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01000068">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002131878.2">
        <property type="nucleotide sequence ID" value="XM_002131842.5"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009862156.1">
        <property type="nucleotide sequence ID" value="XM_009863854.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009862157.1">
        <property type="nucleotide sequence ID" value="XM_009863855.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1W3JJA3"/>
    <dbReference type="Ensembl" id="ENSCINT00000009843.3">
        <property type="protein sequence ID" value="ENSCINP00000009843.3"/>
        <property type="gene ID" value="ENSCING00000004761.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100178806"/>
    <dbReference type="KEGG" id="cin:100178806"/>
    <dbReference type="InParanoid" id="A0A1W3JJA3"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014069081" evidence="2">
        <location>
            <begin position="22"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="49"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000009843.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="107" mass="12271" checksum="814D705B5FA16401" modified="2017-06-07" version="1">MDRKIVFILLLVLLHITAVMANWSTGLRCAADALGRRRRRSRSQRRSRSSSSGTTGSSSRSSMRRRRRRRRSSNEEIESDEFASEIADALSAAEMEEVMQNIMDHQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>F6PHN6</accession>
    <name>F6PHN6_CIOIN</name>
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        <recommendedName>
            <fullName evidence="3">Neuferricin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Cytochrome b5 domain-containing protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100182774</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
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    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Heme-binding protein which promotes neuronal but not astrocyte differentiation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytochrome b5 family. MAPR subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="F6PHN6"/>
    <dbReference type="FunCoup" id="F6PHN6">
        <property type="interactions" value="237"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000006292"/>
    <dbReference type="Ensembl" id="ENSCINT00000006292.3">
        <property type="protein sequence ID" value="ENSCINP00000006292.3"/>
        <property type="gene ID" value="ENSCING00000003108.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160156"/>
    <dbReference type="HOGENOM" id="CLU_065455_0_0_1"/>
    <dbReference type="InParanoid" id="F6PHN6"/>
    <dbReference type="OMA" id="GHKHYGP"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050577">
        <property type="entry name" value="MAPR/NEUFC/NENF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10281">
        <property type="entry name" value="MEMBRANE-ASSOCIATED PROGESTERONE RECEPTOR COMPONENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10281:SF4">
        <property type="entry name" value="NEUFERRICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003340451" description="Neuferricin" evidence="5">
        <location>
            <begin position="19"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="6">
        <location>
            <begin position="76"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00037690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00039568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00042241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000006292.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="295" mass="32729" checksum="72D3155AF82CE684" modified="2012-04-18" version="2">MGCLRLSFIVIVLAVGAGLYFRSTEDARVKEILNTVLKLTPKPIVDLFQSARDDSHNAKKQEKVTDNEVKSTNRIFSKQDLWDNYRGGDESKGLYLAFFGKVFDVSKGKEHYGPGGGYHFFAGRDGTKGFVTGDFSDEGLTDDIEGMDSKLMIGFDDWIQFYSSSYTYVGTVIGQFYDENGEPTEALKNANKLIAEAKQAKKQEDEENKILPMCNSESSAAKGSRVWCSTKSNSGGVERNWVGVPRQYHPPGATSPRCACVRTDDGKNRGDLDNPYLREYNGCHSTSDSCVIKQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>F6PKL5</accession>
    <accession>A0A1W2WJJ0</accession>
    <name>F6PKL5_CIOIN</name>
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        <submittedName>
            <fullName evidence="14">DnaJ homolog subfamily A member 1-like</fullName>
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    </protein>
    <gene>
        <name evidence="14" type="primary">LOC100186653</name>
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    <organism evidence="14 15">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">wild type</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="EAAA01001782">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002131998.1">
        <property type="nucleotide sequence ID" value="XM_002131962.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6PKL5"/>
    <dbReference type="SMR" id="F6PKL5"/>
    <dbReference type="FunCoup" id="F6PKL5">
        <property type="interactions" value="497"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000018243"/>
    <dbReference type="Ensembl" id="ENSCINT00000018243.3">
        <property type="protein sequence ID" value="ENSCINP00000018243.3"/>
        <property type="gene ID" value="ENSCING00000008988.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100186653"/>
    <dbReference type="KEGG" id="cin:100186653"/>
    <dbReference type="GeneTree" id="ENSGT00940000170598"/>
    <dbReference type="HOGENOM" id="CLU_017633_10_0_1"/>
    <dbReference type="InParanoid" id="F6PKL5"/>
    <dbReference type="OMA" id="SYEYETH"/>
    <dbReference type="OrthoDB" id="550424at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:ATP binding"/>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="DnaJ_zf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000041">
        <property type="entry name" value="Chaperone protein DNAj, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.230.10:FF:000005">
        <property type="entry name" value="DnaJ homolog subfamily A member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.260.20:FF:000003">
        <property type="entry name" value="DnaJ subfamily A member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.230.10">
        <property type="entry name" value="Heat shock protein DnaJ, cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.260.20">
        <property type="entry name" value="Urease metallochaperone UreE, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01152">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012724">
        <property type="entry name" value="DnaJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002939">
        <property type="entry name" value="DnaJ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044713">
        <property type="entry name" value="DNJA1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008971">
        <property type="entry name" value="HSP40/DnaJ_pept-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001305">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036410">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43888">
        <property type="entry name" value="DNAJ-LIKE-2, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01556">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00684">
        <property type="entry name" value="DnaJ_CXXCXGXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57938">
        <property type="entry name" value="DnaJ/Hsp40 cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49493">
        <property type="entry name" value="HSP40/DnaJ peptide-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51188">
        <property type="entry name" value="ZF_CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0488">Methylation</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 10" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="J" evidence="12">
        <location>
            <begin position="6"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="CR-type" evidence="13">
        <location>
            <begin position="126"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="CR-type" evidence="10">
        <location>
            <begin position="126"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="362"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00546"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51188"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000018243.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="403" mass="44891" checksum="7ADF5050953B4DC8" modified="2011-07-27" version="1">MVKETKLYDVLGVSSSASDPELKKAYRKQAIKFHPDKNPDNPAASEKFKEISYAYEVLSDPKKRRIYDEGGDQALKEGSGGGGGFHSAHDLFDMFFGGGGSRSRQPTKGKDVVHQLRVSLEDMYNGATKRLSLQKNVICDKCNGRGGKEGAVSKCMTCRGNGIQVRIQQIGPGMMQQIQSTCRDCDGKGERINAKDRCKTCHGKKVVKQNKILEVHVDKGMNEGQKVVFHGEGDQEPGLEAGDVVIVLVEKEHPTLHRENEDLLMKMDINISEALCGFSRVIRTLDGRDIVVTSLPGEVIKYADIKCVHGEGMPIYRDPFQKGRLIIQFKVNFPENNWITPDSIKKLEKLLPSKEEVIITDDMEEVSLQECDPNHRSKSSGRNAYDEDDEDGPHGRGMQCQTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>F6PM75</accession>
    <name>F6PM75_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">Ribosome production factor 2 homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5 6">Ribosome biogenesis protein RPF2 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100176470</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
            <location evidence="1 6">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the RPF2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="F6PM75"/>
    <dbReference type="FunCoup" id="F6PM75">
        <property type="interactions" value="587"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000025091"/>
    <dbReference type="Ensembl" id="ENSCINT00000025337.2">
        <property type="protein sequence ID" value="ENSCINP00000025091.2"/>
        <property type="gene ID" value="ENSCING00000008090.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000007279"/>
    <dbReference type="HOGENOM" id="CLU_049783_1_1_1"/>
    <dbReference type="InParanoid" id="F6PM75"/>
    <dbReference type="OMA" id="VGLKPMF"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007109">
        <property type="entry name" value="Brix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039770">
        <property type="entry name" value="Rpf2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12728">
        <property type="entry name" value="BRIX DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12728:SF0">
        <property type="entry name" value="RIBOSOME PRODUCTION FACTOR 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04427">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00879">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50833">
        <property type="entry name" value="BRIX"/>
        <property type="match status" value="1"/>
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                <person name="Goodstein D.M."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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    </comment>
    <comment type="subunit">
        <text evidence="11">Interacts with PPIF; the interaction is impaired by CsA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 18">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01000610">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002130023.1">
        <property type="nucleotide sequence ID" value="XM_002129987.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6QRJ2"/>
    <dbReference type="FunCoup" id="F6QRJ2">
        <property type="interactions" value="320"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000013236"/>
    <dbReference type="Ensembl" id="ENSCINT00000013236.4">
        <property type="protein sequence ID" value="ENSCINP00000013236.4"/>
        <property type="gene ID" value="ENSCING00000006424.4"/>
    </dbReference>
    <dbReference type="GeneID" id="100185739"/>
    <dbReference type="KEGG" id="cin:100185739"/>
    <dbReference type="GeneTree" id="ENSGT00390000008708"/>
    <dbReference type="HOGENOM" id="CLU_039456_3_1_1"/>
    <dbReference type="InParanoid" id="F6QRJ2"/>
    <dbReference type="OMA" id="YKGAIWL"/>
    <dbReference type="OrthoDB" id="427452at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990547">
        <property type="term" value="P:mitochondrial phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035435">
        <property type="term" value="P:phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000005">
        <property type="entry name" value="Mitochondrial phosphate carrier protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044677">
        <property type="entry name" value="SLC25A3/Pic2/Mir1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45671">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25 (MITOCHONDRIAL CARRIER PHOSPHATE CARRIER), MEMBER 3, LIKE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45671:SF10">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25 MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 17 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4 17 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 18" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="257"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="309"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="17">
        <location>
            <begin position="57"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="17">
        <location>
            <begin position="154"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="17">
        <location>
            <begin position="255"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00035382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00045773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000013236.4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="355" mass="39286" checksum="DB2B1709E77E7CE6" modified="2012-04-18" version="2">MVKTLLEAFENKPNPFNAPKFEVGCTPCEPMKETQISRRIGAASASDEYSCEFGSTKYYTICGFGGILSCGLTHTALVPLDLVKCRIQVDPQKYKNIFHGFKVTLAEDGVRGLAKGWAPTFIGYSMQGLCKFGLYEVFKNIYSGVLGEEKSYLWRTSLYLAASASAEFFADIALAPMEACKVRIQTQPGFANTLREAFPKMKAAEGMSAFYKGLVPLWLRQIPYTMMKFACFERTVEALYKFVVPKPRADCNKTEQLVVTFVAGYIAGVFCAIVSHPADSVVSLLNKDSGSTPLQALKKLGPKGVWKGLFARIIMIGTLTALQWFIYDSVKVYFKLPRPPPPEMPASLKAKLNAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>F6R1Q7</accession>
    <accession>A0A1W2WD11</accession>
    <name>F6R1Q7_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">palmitoyl-protein hydrolase</fullName>
            <ecNumber evidence="2">3.1.2.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100185783</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>S-hexadecanoyl-L-cysteinyl-[protein] + H2O = L-cysteinyl-[protein] + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19233"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="3.1.2.22"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:19234"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DeSI family.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.22" evidence="2"/>
    <dbReference type="RefSeq" id="XP_002126976.1">
        <property type="nucleotide sequence ID" value="XM_002126940.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6R1Q7"/>
    <dbReference type="FunCoup" id="F6R1Q7">
        <property type="interactions" value="64"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000001510"/>
    <dbReference type="MEROPS" id="C97.002"/>
    <dbReference type="Ensembl" id="ENSCINT00000001510.3">
        <property type="protein sequence ID" value="ENSCINP00000001510.3"/>
        <property type="gene ID" value="ENSCING00000000830.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100185783"/>
    <dbReference type="KEGG" id="cin:100185783"/>
    <dbReference type="GeneTree" id="ENSGT00730000111005"/>
    <dbReference type="HOGENOM" id="CLU_069001_5_1_1"/>
    <dbReference type="InParanoid" id="F6R1Q7"/>
    <dbReference type="OMA" id="PLEGCRW"/>
    <dbReference type="OrthoDB" id="412286at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0101005">
        <property type="term" value="F:deubiquitinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008474">
        <property type="term" value="F:palmitoyl-(protein) hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060027">
        <property type="term" value="P:convergent extension involved in gastrulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1720.30">
        <property type="entry name" value="PPPDE domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008580">
        <property type="entry name" value="PPPDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042266">
        <property type="entry name" value="PPPDE_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12378">
        <property type="entry name" value="DESUMOYLATING ISOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12378:SF80">
        <property type="entry name" value="IP06716P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05903">
        <property type="entry name" value="Peptidase_C97"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01179">
        <property type="entry name" value="DUF862"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51858">
        <property type="entry name" value="PPPDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPPDE" evidence="6">
        <location>
            <begin position="1"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000001510.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="174" mass="19940" checksum="BFE27199B7F1EF51" modified="2012-04-18" version="2">MSNCVIYFSVQYWINEYISGLGIGVYHTGIEVYNREFAYGGHQFPFTGVFEIIPRDASDLGETFRFKDSIVLGMTDFTQSDVEKIVEQLGKEYKGCAYHLMHKNCNHFTSALSQILCGRSIPRWINRLAYMSTCVPFLQRCLPQEWLTPVALANHIGEQHDQFSDFTSTTQGRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>F6R213</accession>
    <name>F6R213_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Fanconi anemia group D2 protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="7">wild type</strain>
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    <reference evidence="7" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
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    <comment type="similarity">
        <text evidence="5">Belongs to the Fanconi anemia protein FANCD2 family.</text>
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        <property type="project" value="GO_Central"/>
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        <property type="term" value="P:double-strand break repair involved in meiotic recombination"/>
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        <property type="term" value="P:mitotic intra-S DNA damage checkpoint signaling"/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
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                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
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                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
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                <person name="Medina M."/>
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                <person name="Mount S."/>
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                <person name="Sasaki A."/>
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                <person name="Shin-i T."/>
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                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="nucleotide sequence ID" value="XM_002122243.5"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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        <source>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
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                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
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                <person name="De Tomaso A."/>
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                <person name="Di Gregorio A."/>
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                <person name="Kawashima T."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="De Tomaso A."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
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                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
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                <person name="Roe B.A."/>
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                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">wild type</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12 13">Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val).</text>
    </comment>
    <comment type="function">
        <text evidence="11">Protects eukaryotic initiation factor EIF2S1 from translation-inhibiting phosphorylation by inhibitory kinases such as EIF2AK2/PKR and EIF2AK1/HCR. Plays a critical role in the regulation of protein synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12 13">
            <text>Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.</text>
            <dbReference type="EC" id="3.4.11.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <cofactor evidence="12">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <cofactor evidence="12">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="12">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="12">Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe(2+)-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5 12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the peptidase M24A family. Methionine aminopeptidase eukaryotic type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.18" evidence="12"/>
    <dbReference type="EMBL" id="EAAA01002668">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6S6E8"/>
    <dbReference type="FunCoup" id="F6S6E8">
        <property type="interactions" value="442"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000011313"/>
    <dbReference type="Ensembl" id="ENSCINT00000011313.3">
        <property type="protein sequence ID" value="ENSCINP00000011313.3"/>
        <property type="gene ID" value="ENSCING00000005470.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155016"/>
    <dbReference type="HOGENOM" id="CLU_015857_7_1_1"/>
    <dbReference type="InParanoid" id="F6S6E8"/>
    <dbReference type="OMA" id="GNGWVYD"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004239">
        <property type="term" value="F:initiator methionyl aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008235">
        <property type="term" value="F:metalloexopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01088">
        <property type="entry name" value="MetAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000106">
        <property type="entry name" value="Methionine aminopeptidase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.230.10:FF:000003">
        <property type="entry name" value="Methionine aminopeptidase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03175">
        <property type="entry name" value="MetAP_2_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050247">
        <property type="entry name" value="Met_Aminopeptidase_Type2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001714">
        <property type="entry name" value="Pept_M24_MAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002468">
        <property type="entry name" value="Pept_M24A_MAP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018349">
        <property type="entry name" value="Pept_M24A_MAP2_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00501">
        <property type="entry name" value="met_pdase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45777">
        <property type="entry name" value="METHIONINE AMINOPEPTIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45777:SF2">
        <property type="entry name" value="METHIONINE AMINOPEPTIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00599">
        <property type="entry name" value="MAPEPTIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01202">
        <property type="entry name" value="MAP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12 13" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="7 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 12 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 12 13" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M24" evidence="15">
        <location>
            <begin position="163"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="24"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="31"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="58"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="93"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="245"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="256"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="256"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="325"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="333"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="358"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="455"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="455"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00056196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000011313.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="474" mass="53278" checksum="70FA37466024DDE5" modified="2012-04-18" version="2">MSAVVENGEAENEIELKSIDETIDNESQEVKKKKKKKKKKKKPGEYSCSGKQRSAEVDVGDAGEKEDAEEQGEEVKEDGEAAVNGEGEQTEGKKRRRRKKKTAKQQTNPPTVPVSELFVNKPFPVGEECEYPADKDGRSAAWRTTSEEKKAMNTSYEEMWNDFRQAAETHRQVRHHVQEFIKPGLSMIEICERLEQASRNLINENKLEAGLAFPTGCSLNNCAAHYTPNAGDKTVLSYDDVCKIDFGTHINGRIIDCAFTVSFNPKYDRLLEAVKDATNTGIKNAGIDVRLCDVGAAIQETMESYEVEIDGKTYQVRPIRNLSGHSIGQYRIHAGKTVPIVKGGDQTRMEEGEVFAIETFGSTGKGYVHDDMDCSHYMKNFDLANQHVPLRLPRAKRLLHVINENFGTLAFCRRWIDRIGETKYLMALKNLCDSGVVDAYPPLCDIKGCYTAQFEHTILMRPNCKEVVSRGDDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>F6S9X3</accession>
    <name>F6S9X3_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01002153">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="78"/>
            <end position="100"/>
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        <location>
            <begin position="160"/>
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    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    </evidence>
    <sequence length="218" mass="23692" checksum="594FF8B5412F9FAE" modified="2012-04-18" version="2">MRGPKDCVMMWSDRDGVPFPWISVPCSYTGRYICEKPPSTAPPTSTRAIVATTSEMPSTQQQVTTTDHSEDDVEGFTVFAPIAIILGVAFLAVLSALLICRYFKFIAGAGASARHRANTGLTRENLGNSQIVYRVNTGGEGEHEPSYQEIEVRVSICSVSQNNSERSPSRPESKTMNSEPPSNTLTVPAAVNEASQPLNVENANIEAMYSVVKKTDFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>F6SPG6</accession>
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        <recommendedName>
            <fullName evidence="10">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Tunicata</taxon>
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            <taxon>Phlebobranchia</taxon>
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    </organism>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">wild type</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001388">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="Ensembl" id="ENSCINT00000022296.2">
        <property type="protein sequence ID" value="ENSCINP00000022050.2"/>
        <property type="gene ID" value="ENSCING00000011576.2"/>
    </dbReference>
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    <dbReference type="HOGENOM" id="CLU_009579_6_1_1"/>
    <dbReference type="InParanoid" id="F6SPG6"/>
    <dbReference type="OMA" id="IENDTIC"/>
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        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032870">
        <property type="term" value="P:cellular response to hormone stimulus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45695:SF28">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 1 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45695">
        <property type="entry name" value="LEUCOKININ RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
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    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 8 9" id="KW-0812">Transmembrane</keyword>
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    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
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            <begin position="42"/>
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    <feature type="transmembrane region" description="Helical" evidence="9">
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            <begin position="83"/>
            <end position="100"/>
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            <begin position="121"/>
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            <begin position="172"/>
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        <location>
            <begin position="22"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000022050.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="305" mass="34428" checksum="6B10A2100ECF3E83" modified="2012-04-18" version="2">PNSYVTILIVLYSIIMTVGILGNLCVIVVISSSRKLWKVVNFLLLSLACSDLLLALTLPWTIIHNTSYDYTMGSGLCKAIPPLQGTAVMSSINCLVAIALERHQAIVYPTRQNIFRSHLRLMIAVALIWICSFCFQIPQIVVLDDVVWKQKTPVKPATVCCQETWAPQQSQAYSIAIIVIVYGVPLALLVLLYGRIGYFVFSRRGIENSLSAERSERSRSRRIRLNVMLVLLVTSFALLWGPYFVFQCLTVCLTSYCSPELVGYLQLVGHVNVALDPLLYAALHERVREEFIFLVRRLFRCKLQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>F6SVW0</accession>
    <accession>A0A1W5BJ17</accession>
    <name>F6SVW0_CIOIN</name>
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        <submittedName>
            <fullName evidence="5">Leucine-rich repeat-containing protein 59-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100186997</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
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                <person name="Chin-Bow S."/>
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                <person name="Haga S."/>
                <person name="Hayashi H."/>
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                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
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                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
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                <person name="Doggett N."/>
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                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <text evidence="1">Essential for spermiogenesis.</text>
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        <text evidence="2">Belongs to the MEIG1 family.</text>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Davidson B."/>
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                <person name="Yoshizaki F."/>
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                <person name="Larimer F."/>
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                <person name="Richardson P."/>
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    <reference evidence="4" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Ogasawara M."/>
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                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4">wild type</strain>
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    <reference evidence="4" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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            <dbReference type="PROSITE" id="PS50127"/>
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        <source>
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    <sequence length="306" mass="34037" checksum="0B3205772D600699" modified="2011-07-27" version="1">MQQSYNLRSPAVKRLMREAQELAAPTYEYSAKPLDDNLFEWHFTFRGPKDSDFDGGLYHGRIILPPEYPMKPPSIMLLNANGRFELNKKICLSISGYHPESWQPSWSIRTAILAIIGFMPTKGEGAIGALDYTAEERKVLARRSKSYSCTACGCIKDLLPSEAEGIACTTSDAENRDKELAKQITFGKPKTEKKEDASKSEETLNATPVDPAITPQENNVNTTSENSENPSVENDVSHAQEDGVRKRSTMTTPPPPTTAPLPRLQTNQSSDSSTMLIVLLAIAIALLFLRRIDRMYNLSSMVTDYL</sequence>
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    <accession>F6TLI7</accession>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="Ensembl" id="ENSCINT00000007185.3">
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        <property type="gene ID" value="ENSCING00000013749.2"/>
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    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
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    </dbReference>
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        <property type="entry name" value="MAM_domain-containing"/>
    </dbReference>
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        <property type="entry name" value="APICAL ENDOSOMAL GLYCOPROTEIN PRECURSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23282:SF101">
        <property type="entry name" value="MAM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00629">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MAM_2"/>
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    </feature>
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        </location>
    </feature>
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        <source>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000007185.3"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>F6TUF1</accession>
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            <fullName evidence="9">Uncharacterized protein</fullName>
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        <name type="scientific">Ciona intestinalis</name>
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    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
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                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Putnam N."/>
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                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
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                <person name="Francino P."/>
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                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
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                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
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                <person name="Ohta F."/>
                <person name="Oishi K."/>
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                <person name="Sasakura Y."/>
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                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
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                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
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                <person name="Richardson P."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
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            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">wild type</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="EMBL" id="EAAA01001292">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="F6TUF1">
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="20" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="20">wild type</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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    <comment type="function">
        <text evidence="15">Subunit delta, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. ATP synthase complex consist of a soluble F(1) head domain - the catalytic core - and a membrane F(1) domain - the membrane proton channel. These two domains are linked by a central stalk rotating inside the F(1) region and a stationary peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. In vivo, can only synthesize ATP although its ATP hydrolase activity can be activated artificially in vitro. With the central stalk subunit gamma, is essential for the biogenesis of F(1) catalytic part of the ATP synthase complex namely in the formation of F1 assembly intermediate.</text>
    </comment>
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        <text evidence="16">Component of the ATP synthase complex composed at least of ATP5F1A/subunit alpha, ATP5F1B/subunit beta, ATP5MC1/subunit c (homooctomer), MT-ATP6/subunit a, MT-ATP8/subunit 8, ATP5ME/subunit e, ATP5MF/subunit f, ATP5MG/subunit g, ATP5MK/subunit k, ATP5MJ/subunit j, ATP5F1C/subunit gamma, ATP5F1D/subunit delta, ATP5F1E/subunit epsilon, ATP5PF/subunit F6, ATP5PB/subunit b, ATP5PD/subunit d, ATP5PO/subunit OSCP. ATP synthase complex consists of a soluble F(1) head domain (subunits alpha(3) and beta(3)) - the catalytic core - and a membrane F(0) domain - the membrane proton channel (subunits c, a, 8, e, f, g, k and j). These two domains are linked by a central stalk (subunits gamma, delta, and epsilon) rotating inside the F1 region and a stationary peripheral stalk (subunits F6, b, d, and OSCP). Component of a complex composed at least by ATPIF1, ATP5F1A, ATP5F1B, ATP5F1C AND ATP5F1E.</text>
    </comment>
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            <location evidence="1">Mitochondrion inner membrane</location>
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    </comment>
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        <text evidence="2">Belongs to the ATPase epsilon chain family.</text>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF46604">
        <property type="entry name" value="Epsilon subunit of F1F0-ATP synthase C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51344">
        <property type="entry name" value="Epsilon subunit of F1F0-ATP synthase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="12" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="11" id="KW-0139">CF(1)</keyword>
    <keyword evidence="4" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ATP synthase F1 complex delta/epsilon subunit N-terminal" evidence="18">
        <location>
            <begin position="57"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="F1F0-ATP synthase delta subunit C-terminal" evidence="19">
        <location>
            <begin position="131"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00056834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00062932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00070799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF02823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF21335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000022863.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="172" mass="18181" checksum="AFC9EE1C0BA46D97" modified="2011-07-27" version="1">MSSVVLRLCSRTARAVTRVAAFKPQHRNYAEAAAAVAQPGQMLFTFAAPAEAYYESSTAVRQVDVSTSTGSIGILANHVPTLGVLQPGVITVYETEGAVKKFFVSSGSYCVNEDASVQINAEEAVPIEQLDKDLARANLTKAQSQLSSATTETGKVEAQIAIECNEAIINAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>F6TWF6</accession>
    <name>F6TWF6_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">aldehyde dehydrogenase (NAD(+))</fullName>
            <ecNumber evidence="5">1.2.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100184485</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="2">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.3" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="F6TWF6"/>
    <dbReference type="FunCoup" id="F6TWF6">
        <property type="interactions" value="358"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000015438"/>
    <dbReference type="Ensembl" id="ENSCINT00000015438.3">
        <property type="protein sequence ID" value="ENSCINP00000015438.3"/>
        <property type="gene ID" value="ENSCING00000007517.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154938"/>
    <dbReference type="HOGENOM" id="CLU_005391_1_2_1"/>
    <dbReference type="InParanoid" id="F6TWF6"/>
    <dbReference type="OMA" id="DAWKVYM"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004029">
        <property type="term" value="F:aldehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07130">
        <property type="entry name" value="ALDH_F7_AASADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000018">
        <property type="entry name" value="Alpha-aminoadipic semialdehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044638">
        <property type="entry name" value="ALDH7A1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43521">
        <property type="entry name" value="ALPHA-AMINOADIPIC SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43521:SF1">
        <property type="entry name" value="ALPHA-AMINOADIPIC SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="3 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="8">
        <location>
            <begin position="60"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000015438.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="542" mass="58610" checksum="96DA2888C1616CB0" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>F6ULW5</accession>
    <name>F6ULW5_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="F6ULW5"/>
    <dbReference type="STRING" id="7719.ENSCINP00000027146"/>
    <dbReference type="Ensembl" id="ENSCINT00000027392.2">
        <property type="protein sequence ID" value="ENSCINP00000027146.2"/>
        <property type="gene ID" value="ENSCING00000000539.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160549"/>
    <dbReference type="HOGENOM" id="CLU_020415_1_2_1"/>
    <dbReference type="InParanoid" id="F6ULW5"/>
    <dbReference type="OMA" id="MDICEPQ"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015280">
        <property type="term" value="F:ligand-gated sodium channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="Gene3D" id="2.60.470.10">
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    <accession>F6UY72</accession>
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        <name type="scientific">Ciona intestinalis</name>
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            <taxon>Ciona</taxon>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="8" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">wild type</strain>
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    <reference evidence="8" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="8" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
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        <scope>IDENTIFICATION</scope>
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    <sequence length="777" mass="89415" checksum="35256C957A22756A" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
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            <fullName evidence="10">VWFD domain-containing protein</fullName>
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        <name type="synonym">Ascidia intestinalis</name>
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    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
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                <person name="Manohar C."/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
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                <person name="Doyle S."/>
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                <person name="Detter C."/>
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                <person name="Richardson P."/>
                <person name="Lucas S."/>
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                <person name="Levine M."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
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            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
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    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Hastings K.E."/>
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                <person name="Meinertzhagen I.A."/>
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                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
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                <person name="DeSantis R."/>
                <person name="Doyle S."/>
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                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
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                <person name="Kobayashi K."/>
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                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
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                <person name="Mount S."/>
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                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">wild type</strain>
        </source>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002037">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="EAAA01002038">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6V8A8"/>
    <dbReference type="FunCoup" id="F6V8A8">
        <property type="interactions" value="25"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSCINT00000010841.3">
        <property type="protein sequence ID" value="ENSCINP00000010841.3"/>
        <property type="gene ID" value="ENSCING00000005266.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183024"/>
    <dbReference type="HOGENOM" id="CLU_009772_1_0_1"/>
    <dbReference type="InParanoid" id="F6V8A8"/>
    <dbReference type="OMA" id="IPLMRVI"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004697">
        <property type="term" value="F:diacylglycerol-dependent serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd20795">
        <property type="entry name" value="C1_PKD_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20796">
        <property type="entry name" value="C1_PKD_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01239">
        <property type="entry name" value="PH_PKD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14082">
        <property type="entry name" value="STKc_PKD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000151">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000021">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020454">
        <property type="entry name" value="DAG/PE-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057764">
        <property type="entry name" value="Ubiquitin_PRKD1-3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22968">
        <property type="entry name" value="PROTEIN KINASE C, MU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22968:SF24">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25525">
        <property type="entry name" value="Ubiquitin_PRKD1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00008">
        <property type="entry name" value="DAGPEDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="16" id="KW-0460">Magnesium</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="14" id="KW-0862">Zinc</keyword>
    <keyword evidence="12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="21">
        <location>
            <begin position="96"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="21">
        <location>
            <begin position="217"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="20">
        <location>
            <begin position="495"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="524"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000010841.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="790" mass="90722" checksum="706850ABA6FCAAB7" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>F6V9J0</accession>
    <name>F6V9J0_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="21">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the dynein heavy chain family.</text>
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        <property type="protein sequence ID" value="ENSCINP00000008501.3"/>
        <property type="gene ID" value="ENSCING00000004123.3"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:9+2 motile cilium"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
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        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:dynein intermediate chain binding"/>
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        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0051959">
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        <property type="project" value="GO_Central"/>
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        <property type="term" value="F:minus-end-directed microtubule motor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060294">
        <property type="term" value="P:cilium movement involved in cell motility"/>
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        <property type="project" value="GO_Central"/>
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        <property type="entry name" value="Dynein axonemal heavy chain 2"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Dynein heavy chain 4"/>
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    </dbReference>
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        <property type="entry name" value="Dynein heavy chain 5, axonemal"/>
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        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="6"/>
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        <property type="entry name" value="AAA_6"/>
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        <property type="entry name" value="AAA_9"/>
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        <property type="entry name" value="DHC"/>
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        <property type="entry name" value="Dynein_AAA1S"/>
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        <property type="entry name" value="Dynein_AAA5_ext"/>
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        <property type="entry name" value="Dynein_C_barrel"/>
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        <property type="entry name" value="Dynein_heavy_chain_D4_dom"/>
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        <property type="entry name" value="P-loop_NTPase"/>
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        <property type="entry name" value="DYNEIN AXONEMAL HEAVY CHAIN 14"/>
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    </dbReference>
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        <property type="entry name" value="DYNEIN HEAVY CHAIN 6, AXONEMAL-LIKE-RELATED"/>
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        <property type="entry name" value="AAA_8"/>
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        <property type="entry name" value="AAA_9"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Dynein_AAA_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Dynein_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="3"/>
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    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="10" id="KW-0969">Cilium</keyword>
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    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
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    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
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        <location>
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            <end position="947"/>
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        <location>
            <begin position="981"/>
            <end position="1201"/>
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    <feature type="domain" description="Dynein heavy chain AAA 5 extension" evidence="19">
        <location>
            <begin position="1432"/>
            <end position="1615"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain AAA module D4" evidence="17">
        <location>
            <begin position="1778"/>
            <end position="2018"/>
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    </feature>
    <feature type="domain" description="Dynein heavy chain coiled coil stalk" evidence="16">
        <location>
            <begin position="2032"/>
            <end position="2271"/>
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    </feature>
    <feature type="domain" description="Dynein heavy chain ATP-binding dynein motor region" evidence="18">
        <location>
            <begin position="2359"/>
            <end position="2462"/>
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    <feature type="domain" description="Dynein heavy chain C-terminal" evidence="20">
        <location>
            <begin position="2838"/>
            <end position="3237"/>
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        <location>
            <begin position="302"/>
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            <begin position="264"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00008887"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
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        <source>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
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        <source>
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    </evidence>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="Pfam" id="PF18199"/>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>F6VB18</accession>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="ENSCINP00000022554.2"/>
        <property type="gene ID" value="ENSCING00000011931.2"/>
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        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="26"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000022554.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="83" mass="9469" checksum="452276222A3FE7A6" modified="2011-07-27" version="1">MVAMQVPDKLSIITYVSQYYNRLHKMKPADDEKPLKRKSTLQSTPEFVKIKRKEPEPVKKVGSTSQPLAINSNFTRPSLPSKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>F6VJP5</accession>
    <name>F6VJP5_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
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                <person name="De Tomaso A."/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="CARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01670">
        <property type="entry name" value="Death"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001315">
        <property type="entry name" value="CARD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000488">
        <property type="entry name" value="Death_dom"/>
    </dbReference>
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        <property type="entry name" value="CARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00531">
        <property type="entry name" value="Death"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50209">
        <property type="entry name" value="CARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50017">
        <property type="entry name" value="DEATH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CARD" evidence="3">
        <location>
            <begin position="40"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="Death" evidence="2">
        <location>
            <begin position="164"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="286"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000003147.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="405" mass="46484" checksum="70FB8761196CAC76" modified="2012-04-18" version="2">MYTSLKKHVSSSVKGTYKKKDWSLDFERLPWIQWLNIEPMTTRQKTAFQLLYPILLQEINTKKPSKLLHYLVGKGVLFWQDVVVIQHGRQTDLERNSPLLKIIWTRGKAGFTGLVDGLRSRYGGWQGYLADMLELKYNMANTRDMMDIYKVICHDLVKVMPVGWSALPRVLGMSNSHIEKCRRENKLLANQLLHCLLISSSQHFNKDHLLSKLLSALHVINRDDIADGIAILLNTMYEIQSVHQENMASDETKETNNTALDEIDAQNEETVEVVFQQQNNGVQLKSASSFCSDGETPRADSGFDSRMSTSTPITLYPPTPPPHSSNQRYHHRSSSKKFYRKTPSLEKFEGLSNIGRVKSGRLHGYRISPRAKTNAWERRSPSILQADYLAHPNQDNLFMDYLVYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>F6W5E5</accession>
    <name>F6W5E5_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glycosyl hydrolase family 31 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01002402">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6W5E5"/>
    <dbReference type="STRING" id="7719.ENSCINP00000016088"/>
    <dbReference type="Ensembl" id="ENSCINT00000016088.3">
        <property type="protein sequence ID" value="ENSCINP00000016088.3"/>
        <property type="gene ID" value="ENSCING00000011963.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163043"/>
    <dbReference type="HOGENOM" id="CLU_1383744_0_0_1"/>
    <dbReference type="InParanoid" id="F6W5E5"/>
    <dbReference type="OMA" id="VAINIRY"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016798">
        <property type="term" value="F:hydrolase activity, acting on glycosyl bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048395">
        <property type="entry name" value="Glyco_hydro_31_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22762">
        <property type="entry name" value="ALPHA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22762:SF133">
        <property type="entry name" value="P-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21365">
        <property type="entry name" value="Glyco_hydro_31_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyl hydrolase family 31 C-terminal" evidence="1">
        <location>
            <begin position="2"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF21365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000016088.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="197" mass="22408" checksum="A94329A8D488246A" modified="2011-07-27" version="1">MFLLGPAFLVTPVLDEGEVEVTGYFPQQSRWFDFRTGMEQVTGKFLTLDAPMDYINLHVRGGYILPMQGPDVTTTLSRKKPMEILVALDDSQSAAGELYWDDGEARNVGENFTRIEFKFGLNVLNFTVVHQAEYLPELDYLYIDKLTVYGLLSEPSTITCDGNDEASFSWESVRNKLFLNLFSTLNLTDDHQCVFTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>F6WKS5</accession>
    <name>F6WKS5_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Inward rectifier potassium channel C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">wild type</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001989">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6WKS5"/>
    <dbReference type="STRING" id="7719.ENSCINP00000010507"/>
    <dbReference type="Ensembl" id="ENSCINT00000010507.3">
        <property type="protein sequence ID" value="ENSCINP00000010507.3"/>
        <property type="gene ID" value="ENSCING00000005094.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01140000282491"/>
    <dbReference type="HOGENOM" id="CLU_2132638_0_0_1"/>
    <dbReference type="InParanoid" id="F6WKS5"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034702">
        <property type="term" value="C:monoatomic ion channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005242">
        <property type="term" value="F:inward rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1400">
        <property type="entry name" value="G protein-activated inward rectifier potassium channel 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041647">
        <property type="entry name" value="IRK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016449">
        <property type="entry name" value="K_chnl_inward-rec_Kir"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013518">
        <property type="entry name" value="K_chnl_inward-rec_Kir_cyto"/>
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01001825">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6XDY8"/>
    <dbReference type="Ensembl" id="ENSCINT00000023722.2">
        <property type="protein sequence ID" value="ENSCINP00000023476.2"/>
        <property type="gene ID" value="ENSCING00000012623.2"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1574207_0_0_1"/>
    <dbReference type="InParanoid" id="F6XDY8"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="120"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000023476.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="170" mass="19598" checksum="285BE84EEFC0FF3C" modified="2011-07-27" version="1">MLIVVVIQSFLGLYICVVLVYYECQAMPKNLGRIQRKAKRLRCLLTFCATVGVVYFSIALTAIINSRFLQSNQACQVLVVAQKLTYSVVLYSLYGFLWLRQKMFYDNPSMENLYNRKLRIASWGVFVLAILCFLIQAVLTLLGSKYQVKNKPPGVCYEQERWLSESQIIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>F6XE19</accession>
    <accession>A0A1W2W4B9</accession>
    <name>F6XE19_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Metalloendopeptidase</fullName>
            <ecNumber evidence="7">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100186632</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6 7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="6 7">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="7"/>
    <dbReference type="AlphaFoldDB" id="F6XE19"/>
    <dbReference type="Ensembl" id="ENSCINT00000023718.2">
        <property type="protein sequence ID" value="ENSCINP00000023472.2"/>
        <property type="gene ID" value="ENSCING00000012619.2"/>
    </dbReference>
    <dbReference type="GeneID" id="100186632"/>
    <dbReference type="KEGG" id="cin:100186632"/>
    <dbReference type="GeneTree" id="ENSGT00940000168869"/>
    <dbReference type="HOGENOM" id="CLU_919892_0_0_1"/>
    <dbReference type="InParanoid" id="F6XE19"/>
    <dbReference type="OrthoDB" id="291007at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04280">
        <property type="entry name" value="ZnMc_astacin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000106">
        <property type="entry name" value="Metalloendopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034035">
        <property type="entry name" value="Astacin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001506">
        <property type="entry name" value="Peptidase_M12A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006026">
        <property type="entry name" value="Peptidase_Metallo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10127">
        <property type="entry name" value="DISCOIDIN, CUB, EGF, LAMININ , AND ZINC METALLOPROTEASE DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10127:SF780">
        <property type="entry name" value="METALLOENDOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01400">
        <property type="entry name" value="Astacin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00480">
        <property type="entry name" value="ASTACIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00235">
        <property type="entry name" value="ZnMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51864">
        <property type="entry name" value="ASTACIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 6 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 6 7" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1 6 7" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="4 6 7" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013982327" description="Metalloendopeptidase" evidence="7">
        <location>
            <begin position="20"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M12A" evidence="8">
        <location>
            <begin position="121"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="227"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="236"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000023472.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="303" mass="33829" checksum="E973F360D22C6FA6" modified="2012-04-18" version="1">MKLIIIVAVVVVLVGGAVGAAVSFLQGSSVCNDPESLTDPVKISECLVVNCKFDVSKCTLPCPMGYSTKGGDDPCPKSCKCSAEGSFEGDIQFYDDDIPELLGKYGYVDKDDEEFSLFVGSALYVKGKILWDTKALPSGRIPIGYKLDRFLTVAKRLKKEMAKASKMYSDNTCIDIEPYDREKHGTHYVDITLGQGCSSMVGYKKNIKQQTMSLQYGCHYSGIIVHEFLHTLGFYHEQSRPDRDDFVKIHLDRVKYGNAHNFQKSRAYFVKDLKSPYDLQSIMHYSTREFSKNGQPTITHAGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>F6XK10</accession>
    <name>F6XK10_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">leucine--tRNA ligase</fullName>
            <ecNumber evidence="3">6.1.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Leucyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">wild type</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>tRNA(Leu) + L-leucine + ATP = L-leucyl-tRNA(Leu) + AMP + diphosphate</text>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
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    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="molecule type" value="Genomic_DNA"/>
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            <begin position="30"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000023187.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
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</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>F6Y8Y8</accession>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
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                <person name="De Tomaso A."/>
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                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
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                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
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                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
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                <consortium name="Ensembl"/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="De Tomaso A."/>
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                <person name="Terry A."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
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                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
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            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
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                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
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                <person name="Doyle S."/>
                <person name="Francino P."/>
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                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
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                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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            </authorList>
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            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">wild type</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="ENSCING00000019499.1"/>
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    <dbReference type="InParanoid" id="F6YR52"/>
    <dbReference type="OMA" id="NTWKINC"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 4"/>
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    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0030247">
        <property type="term" value="F:polysaccharide binding"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="CBM2/CBM3_carb-bd_dom_sf"/>
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        <property type="entry name" value="CBM2_carb-bd_dom_sf"/>
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    </dbReference>
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        <property type="entry name" value="Carbohydrate-binding domain"/>
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        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="chain" id="PRO_5003346199" description="N-acetyl-beta-glucosaminidase" evidence="2">
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            <end position="253"/>
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            <begin position="37"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00033000"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01081"/>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000011163.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="253" mass="28953" checksum="36B37EBE6F90F482" modified="2012-04-18" version="2">MKQGTKMWHLLCAIICLLHAPQLSECLAQSDLNYIGENLNVQFNVINNLNGNNENFNAEVLLHNTGPSVINKDSWEIFFYSLYMIEPDHLPNIAGYEIPQTGLTFYHVNGILFKFSPTANFKSLQPGATLTVSLVIQYWSVSRTDHMPRWYVASQGFLPVMLKSTESDSEFVGSFDTKDKYKRYADDRYHPFSGEDRYARFSSMNHEATPKPIIPTPTEITVENADTLVDNTWKINCLEPFYQKECQLLSGIF</sequence>
</entry>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="protein sequence ID" value="ENSCINP00000000593.2"/>
        <property type="gene ID" value="ENSCING00000000330.2"/>
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        <location>
            <begin position="209"/>
            <end position="241"/>
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            <begin position="337"/>
            <end position="361"/>
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        <location>
            <begin position="209"/>
            <end position="224"/>
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            <begin position="300"/>
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            <begin position="350"/>
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    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000000593.2"/>
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    </evidence>
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        <source>
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    <sequence length="443" mass="48788" checksum="4585F40E229730CD" modified="2011-07-27" version="1">MSLILQSETQQVVKHGQSNGISFNEIENTDAVQKEPSSPIINNEVNLYHQTTLGDEIPTVDNEDTFYKNICQQVVSGDDLTSLLNETSVTQVMKEVNQTTNTSTPATESFTTISANDGLFLFLDETQTKEYCDAPSKATDTLKEWLNKTSDKIEPVIQVLPNVTVRPQYKNKYRKLLPKPASGQSTPVHTPSKNHVRSLAATFNPSNSSAFYQVQKPSPSTNDPAQPHFKPEEINSAGSPLLLPSVLGSSGIPVKISSRSVEMNTGSSGGVWKTPGSSKDVGRTLGSSKDVGKTLGSSKDAGKTPEDDRKTVRDSNNSFKERICSVQLEDCMKVELSESETDISSQDLQNHPENRVDDVGKDPALNKARRFELDDEKRWLTSTPSLVQLFSPQPPCNSLGGVVETPSIHNGVSEKYKQKKSLRVKLQEKDETDPRNLFYNLGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>F6YXT8</accession>
    <accession>A0A1W2WA85</accession>
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            <fullName evidence="11">mRNA cap guanine-N(7) methyltransferase</fullName>
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        <name evidence="16" type="primary">LOC100184027</name>
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    <organism evidence="16 17">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
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    <reference evidence="17" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
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                <person name="Satake M."/>
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                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
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                <person name="Inaba K."/>
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                <person name="Manohar C."/>
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                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="10">Catalytic subunit of the mRNA-capping methyltransferase RNMT:RAMAC complex that methylates the N7 position of the added guanosine to the 5'-cap structure of mRNAs. Binds RNA containing 5'-terminal GpppC.</text>
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    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a 5'-end (5'-triphosphoguanosine)-ribonucleoside in mRNA + S-adenosyl-L-methionine = a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in mRNA + S-adenosyl-L-homocysteine</text>
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            <dbReference type="Rhea" id="RHEA-COMP:17167"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:156461"/>
            <dbReference type="ChEBI" id="CHEBI:167617"/>
            <dbReference type="EC" id="2.1.1.56"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the class I-like SAM-binding methyltransferase superfamily. mRNA cap 0 methyltransferase family.</text>
    </comment>
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        <property type="gene ID" value="ENSCING00000000315.3"/>
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    <dbReference type="KEGG" id="cin:100184027"/>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:7-methylguanosine mRNA capping"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004971">
        <property type="entry name" value="mRNA_G-N7_MeTrfase_dom"/>
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        <property type="entry name" value="mRNA_G-N7_MeTrfase_euk"/>
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    <dbReference type="InterPro" id="IPR039753">
        <property type="entry name" value="RG7MT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
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                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Ueno K."/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="De Tomaso A."/>
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                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the SERAC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002619">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6ZGK8"/>
    <dbReference type="FunCoup" id="F6ZGK8">
        <property type="interactions" value="30"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000024505"/>
    <dbReference type="Ensembl" id="ENSCINT00000024751.2">
        <property type="protein sequence ID" value="ENSCINP00000024505.2"/>
        <property type="gene ID" value="ENSCING00000013310.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000003560"/>
    <dbReference type="HOGENOM" id="CLU_023317_1_0_1"/>
    <dbReference type="InParanoid" id="F6ZGK8"/>
    <dbReference type="OMA" id="RRTEYIY"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052374">
        <property type="entry name" value="SERAC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48182">
        <property type="entry name" value="PROTEIN SERAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48182:SF2">
        <property type="entry name" value="PROTEIN SERAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="12" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00040991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00041701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000024505.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="639" mass="72891" checksum="AA20F9D2822C6C53" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>F6ZIF8</accession>
    <name>F6ZIF8_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Nuclear transcription factor Y subunit gamma</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">CAAT box DNA-binding protein subunit C</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Nuclear transcription factor Y subunit C</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">wild type</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes. NF-Y can function as both an activator and a repressor, depending on its interacting cofactors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Heterotrimeric transcription factor composed of three components, NF-YA, NF-YB and NF-YC. NF-YB and NF-YC must interact and dimerize for NF-YA association and DNA binding.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the NFYC/HAP5 subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002472">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6ZIF8"/>
    <dbReference type="FunCoup" id="F6ZIF8">
        <property type="interactions" value="486"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000015864"/>
    <dbReference type="Ensembl" id="ENSCINT00000015864.3">
        <property type="protein sequence ID" value="ENSCINP00000015864.3"/>
        <property type="gene ID" value="ENSCING00000007727.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155689"/>
    <dbReference type="HOGENOM" id="CLU_045277_2_0_1"/>
    <dbReference type="InParanoid" id="F6ZIF8"/>
    <dbReference type="OMA" id="AWIHTQD"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016602">
        <property type="term" value="C:CCAAT-binding factor complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd22908">
        <property type="entry name" value="HFD_NFYC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000006">
        <property type="entry name" value="Nuclear transcription factor Y subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050568">
        <property type="entry name" value="Transcr_DNA_Rep_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10252">
        <property type="entry name" value="HISTONE-LIKE TRANSCRIPTION FACTOR CCAAT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10252:SF8">
        <property type="entry name" value="NUCLEAR TRANSCRIPTION FACTOR Y SUBUNIT GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="13">
        <location>
            <begin position="20"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00040590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00042333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00042663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000015864.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="263" mass="30093" checksum="61BD085A9E19A524" modified="2012-04-18" version="2">MSTPAEQTPTEAQQLLQGFWQKQLEEVRGLDMNDFKVQDLPLARIKKIMKMDEDVKMISAEAPLLFAKAAQMFITELSLRAWIHTEENKRRTLQRNDIATAITKFDQFDFLIDIVPREDLKQTTRRATDETRGSGENVQYFYTVPQQQVAQQNSTPVINQSSAQTGQIVITQPSNQIVGSDTNQQVLQIGQQQQQQQQQQQPQQQQQVKAKLNSNAVIVGGNNNQQQQQQQQSSVVVNQQQQQQQQQQQQEFINLICTVPEDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>F6ZME0</accession>
    <name>F6ZME0_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Putnam N."/>
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                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="F6ZT29"/>
    <dbReference type="STRING" id="7719.ENSCINP00000019845"/>
    <dbReference type="Ensembl" id="ENSCINT00000019845.3">
        <property type="protein sequence ID" value="ENSCINP00000019845.3"/>
        <property type="gene ID" value="ENSCING00000009771.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000167878"/>
    <dbReference type="HOGENOM" id="CLU_1906013_0_0_1"/>
    <dbReference type="InParanoid" id="F6ZT29"/>
    <dbReference type="OMA" id="TIFHINQ"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24251:SF30">
        <property type="entry name" value="MEMBRANE FRIZZLED-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24251">
        <property type="entry name" value="OVOCHYMASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CUB" evidence="4">
        <location>
            <begin position="39"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000019845.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="154" mass="17047" checksum="452D40F0F08BE420" modified="2012-04-18" version="2">MGNVSEARQFTSRAIRVRFYTDGSITNVGFYATYKISQCGFEATATNTLQTIESPNYPRNYLNNINCGWVVIAPTGRQVIVTVMGGFLEINYDYLRIYDASTLLTQLSGRLTRSSFTSTSGKLRMNFQTDGSVVKTGFRLTYVTQSSSSAPVPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>F7A1T4</accession>
    <name>F7A1T4_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ceramide glucosyltransferase</fullName>
            <ecNumber evidence="5">2.4.1.80</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">wild type</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>N-(9Z-octadecenoyl)-sphing-4-enine + UDP-alpha-D-xylose = beta-D-xylosyl-(1&lt;-&gt;1')-N-(9Z-octadecenoyl)-sphing-4-enine + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:70247"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57632"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:77996"/>
            <dbReference type="ChEBI" id="CHEBI:189081"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:70248"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>UDP-alpha-D-xylose + an N-acylsphing-4-enine = a beta-D-xylosyl-(1&lt;-&gt;1')-N-acylsphing-4-enine + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:70243"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:52639"/>
            <dbReference type="ChEBI" id="CHEBI:57632"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:189068"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:70244"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; sphingolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyltransferase 2 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.80" evidence="5"/>
    <dbReference type="EMBL" id="EAAA01002574">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="F7A1T4">
        <property type="interactions" value="13"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000017071"/>
    <dbReference type="Ensembl" id="ENSCINT00000017071.4">
        <property type="protein sequence ID" value="ENSCINP00000017071.4"/>
        <property type="gene ID" value="ENSCING00000008365.4"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000012898"/>
    <dbReference type="HOGENOM" id="CLU_030898_0_0_1"/>
    <dbReference type="InParanoid" id="F7A1T4"/>
    <dbReference type="OMA" id="IVWIIDC"/>
    <dbReference type="UniPathway" id="UPA00222"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008120">
        <property type="term" value="F:ceramide glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006679">
        <property type="term" value="P:glucosylceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02520">
        <property type="entry name" value="Glucosylceramide_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000041">
        <property type="entry name" value="UDP-glucose ceramide glucosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025993">
        <property type="entry name" value="Ceramide_glucosylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12726">
        <property type="entry name" value="CERAMIDE GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12726:SF0">
        <property type="entry name" value="CERAMIDE GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13506">
        <property type="entry name" value="Glyco_transf_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="6"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="290"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="320"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000017071.4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="401" mass="46109" checksum="803D470B7BE8B819" modified="2012-04-18" version="2">MDAVSWWWVSMIYLLEGFAAFGIALDIGLLLMHFLSIVYSKLYMNKKPADMMKAEVKGVSILKPLKGVDPNLEDNLETFFELDYPQFELLFCIQDNNDPAIDVVERLIHKYPAVDAKIVSGGKRIGVNPKINNLMPGYNCMKYNLFWICDSGIRVLPLTLRDLVSKMQDKVALVHAVPYTSNRKGFASSIEKIYFGTQHTRVYISAHVIGFTCMTGMSNLIRRDYFDKSTGGLGKLAQYIAEDYFMXKXLSDXYSGYKLTLSSYPALQNSGNYGVGKFIERMTRWTKLRVAMVPVAVILEPFSECFLSGLLFSWSCLHFFSWNMLVVFLYHVLVWFILDYILFRQLENGHLQISKCDFLVAWFVRETLTPYIIFCALWDPTINWRTGKYRLRCGGKAEEVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>F7A6R2</accession>
    <name>F7A6R2_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC113474994</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
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                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein-tyrosine phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="F7AJJ9"/>
    <dbReference type="FunCoup" id="F7AJJ9">
        <property type="interactions" value="1"/>
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    <dbReference type="STRING" id="7719.ENSCINP00000005095"/>
    <dbReference type="Ensembl" id="ENSCINT00000005095.3">
        <property type="protein sequence ID" value="ENSCINP00000005095.3"/>
        <property type="gene ID" value="ENSCING00000002433.3"/>
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    <dbReference type="GeneTree" id="ENSGT00940000166600"/>
    <dbReference type="HOGENOM" id="CLU_001645_0_2_1"/>
    <dbReference type="InParanoid" id="F7AJJ9"/>
    <dbReference type="OMA" id="IFIHEAV"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
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    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="P:axon guidance"/>
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        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000062">
        <property type="entry name" value="Receptor-type tyrosine-protein phosphatase kappa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000003">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase kappa isoform X1"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="2.60.40.10:FF:000019">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase kappa isoform X2"/>
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        <property type="entry name" value="receptor-type tyrosine-protein phosphatase U isoform X1"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="2.60.40.10:FF:000048">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase U isoform X1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="4"/>
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    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057598">
        <property type="entry name" value="Fn3_PTPRU"/>
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        <property type="entry name" value="FN3_sf"/>
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        <property type="entry name" value="Ig-like_fold"/>
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        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
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        <property type="entry name" value="Protein-Tyr_Phosphatase"/>
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        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134:SF550">
        <property type="entry name" value="PROTEIN-TYROSINE-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134">
        <property type="entry name" value="RECEPTOR-TYPE TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
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        <property type="entry name" value="Fn3_PTPRU"/>
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        <property type="entry name" value="Y_phosphatase"/>
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    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
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        <property type="entry name" value="PTPc"/>
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    </dbReference>
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        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="7" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="478"/>
            <end position="503"/>
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    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="15">
        <location>
            <begin position="9"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="15">
        <location>
            <begin position="110"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="15">
        <location>
            <begin position="215"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="13">
        <location>
            <begin position="632"/>
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        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="14">
        <location>
            <begin position="809"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="13">
        <location>
            <begin position="921"/>
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        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="14">
        <location>
            <begin position="1103"/>
            <end position="1179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009580"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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            <dbReference type="ARBA" id="ARBA00023180"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00051722"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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            <dbReference type="PROSITE" id="PS50055"/>
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        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000005095.3"/>
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    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="1191" mass="133470" checksum="797B6CEC3E2E38F1" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>F7ALS7</accession>
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        <submittedName>
            <fullName evidence="2">F-box only protein 39-like</fullName>
        </submittedName>
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    <gene>
        <name evidence="2" type="primary">LOC100183530</name>
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    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    <dbReference type="STRING" id="7719.ENSCINP00000010990"/>
    <dbReference type="Ensembl" id="ENSCINT00000010990.2">
        <property type="protein sequence ID" value="ENSCINP00000010990.2"/>
        <property type="gene ID" value="ENSCING00000005348.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00510000048837"/>
    <dbReference type="HOGENOM" id="CLU_050223_0_0_1"/>
    <dbReference type="InParanoid" id="F7ALS7"/>
    <dbReference type="OMA" id="MATFHNL"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
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        <property type="entry name" value="FBOX"/>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
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            <end position="64"/>
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    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000010990.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    </evidence>
    <sequence length="449" mass="52882" checksum="FB82D04E1477E87F" modified="2011-07-27" version="1">CEETKANTRPQTPVSDTKSWDNLPHVSLIKIFTYLKESQLLELQFVCKRWYDVIRHTPSLWREKYFRFSGREPRDLTHQPYRYATHFIRTFGKYLHVLEFRLHSPVSTGVCKKFQVCVKVCLNHLAKSRARIKVLSLPMLQLDRSQWMLYKEDFCSALAKFFCKSQHSMRDVCLRGARANFDDGYKVLYAMGYNTGDTVTRLDLEDFFGRTPTPVYDKPQFVDCLKNFTNLQELDLNYSCLSEELLDGLAEKLNPDSLRWFYIKVYAYDPHNQVVWGHSWGALVKKCPSLKVNMLFQRVMTSDEHFRILLPQIPLSQVSFDGCYFADQSWQLYPTIASILPLFKKHLRRITIDLPDTREVFENELLTLIQHCDKLVSLKLYAFITSRFVDRLLQMVQDGKSKLEIIKIRIYLRDYDTQDEESELDRIFAKYERLVGGDLNYYASCVTSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>F7B8K8</accession>
    <name>F7B8K8_CIOIN</name>
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        <recommendedName>
            <fullName evidence="10">C2H2-type domain-containing protein</fullName>
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    </protein>
    <organism evidence="8 9">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
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    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">wild type</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="EAAA01001372">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="F7B8K8"/>
    <dbReference type="STRING" id="7719.ENSCINP00000015238"/>
    <dbReference type="Ensembl" id="ENSCINT00000015238.3">
        <property type="protein sequence ID" value="ENSCINP00000015238.3"/>
        <property type="gene ID" value="ENSCING00000007420.3"/>
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    <dbReference type="InParanoid" id="F7B8K8"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Znf385"/>
    </dbReference>
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        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23067:SF14">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23067">
        <property type="entry name" value="DOUBLE-STRANDED RNA-BINDING ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
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    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000015238.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="186" mass="20998" checksum="F30C5D5AF68FFE49" modified="2011-07-27" version="1">MKQHVKSLRHKSVAQGIPIPPKPIKDEKVKVKSDQYRCTVCKVCLNSYIQMTQHLSSLRHKNMLEGKPQKPRWCPYERNPPSALTSMSVPFPKSYLSPYFIRSNTIGGQQVSSMATSLLDMQGSAESLLLPQHYMNALTGSSYMKDTPIVLSENRYVEAREVAYIACQPSFPSLPLQPLPQILDFS</sequence>
</entry>
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        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
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    <gene>
        <name evidence="13" type="primary">asak</name>
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        <name type="scientific">Ciona intestinalis</name>
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        <lineage>
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            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
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                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">wild type</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Sulfur metabolism; sulfate assimilation.</text>
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    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the sulfate adenylyltransferase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the APS kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001522">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_018816595.2"/>
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    <dbReference type="FunCoup" id="F7BBN1">
        <property type="interactions" value="100"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000018567"/>
    <dbReference type="Ensembl" id="ENSCINT00000018567.3">
        <property type="protein sequence ID" value="ENSCINP00000018567.3"/>
        <property type="gene ID" value="ENSCING00000009141.3"/>
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    <dbReference type="GeneID" id="445752"/>
    <dbReference type="CTD" id="445752"/>
    <dbReference type="GeneTree" id="ENSGT00390000009613"/>
    <dbReference type="HOGENOM" id="CLU_009463_3_0_1"/>
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    <dbReference type="OMA" id="IEIYKHH"/>
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    <dbReference type="UniPathway" id="UPA00097"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 2"/>
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    <dbReference type="GO" id="GO:0004020">
        <property type="term" value="F:adenylylsulfate kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
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        <name type="scientific">Ciona intestinalis</name>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="3" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="molecule type" value="Genomic_DNA"/>
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</entry>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
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                <person name="De Tomaso A."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
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                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
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                <person name="Wiley G.B."/>
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                <person name="Roe B.A."/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
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                <person name="Doyle S."/>
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                <person name="Haga S."/>
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                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
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                <person name="Nomoto H."/>
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                <person name="Sasaki A."/>
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                <person name="Shoguchi E."/>
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                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
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                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
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                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
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                <person name="Doggett N."/>
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                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <consortium name="Ensembl"/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
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                <person name="Hastings K.E."/>
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                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
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                <person name="Putnam N."/>
                <person name="Rash S."/>
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                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
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                <person name="Shoguchi E."/>
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                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
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                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
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                <person name="Levine M."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="11" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
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            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">wild type</strain>
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    <reference evidence="11" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <name type="scientific">Ciona intestinalis</name>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="ENSCINP00000030293.1"/>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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        <source>
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    <sequence length="148" mass="16785" checksum="D98525CC03AEE771" modified="2012-04-18" version="1">MIVGGWNTQKLVKIYDINRKSFKPLESTLYERYASTSVKINNHVYTAGGHRSKSVERLDLNQVDGGWNEVESMKKQRYLAASAVLNGQMCVAGGWHDGGSNIFSSVELYNPVVNTWTIIPSMKTKRFQHALVSYNGWYNKYKCNDKVG</sequence>
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            <fullName evidence="11">Polyamine-modulated factor 1-like</fullName>
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    </protein>
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        <name evidence="11" type="primary">LOC100175162</name>
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    <organism evidence="11 12">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
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    <reference evidence="12" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
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                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
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                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
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                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
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                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
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                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Centromere</location>
            <location evidence="2">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_002119229.1">
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
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    <accession>H2XLR7</accession>
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        <recommendedName>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
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            <taxon>Phlebobranchia</taxon>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="ENSCINP00000030599.1"/>
        <property type="gene ID" value="ENSCING00000024031.1"/>
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    </dbReference>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ig-like_dom"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="1">
        <location>
            <begin position="59"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000030599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="221" mass="25413" checksum="A3A15F23A741484E" modified="2012-04-18" version="1">MTDGHWRTCDIPNVRRIFTLMILCSLMTLTEARKLNLIRNSQRKCAKMKAKHVYTTPDPGGSYFWDGDVDTTIERHVGDNIKLNCMDLIYVNPIFMGDLNSPPITMKWTKYENDVRAINQHYYPFGCKKRPLLMNTRLYIRNATMNDTGIYSCQAHRANDTEVVQQKLFFVLVVNQEGRSAPITEFPVYGSSDMTLLSGVRHVNSTSSTNRSLRCDYIVRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>H2XN21</accession>
    <name>H2XN21_CIOIN</name>
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        <alternativeName>
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        <alternativeName>
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        <alternativeName>
            <fullName evidence="18">N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase II</fullName>
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    </protein>
    <organism evidence="27 28">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
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                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="27">wild type</strain>
        </source>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>an N(4)-{beta-D-GlcNAc-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc}-L-asparaginyl-[protein] + UDP-N-acetyl-alpha-D-glucosamine = N(4)-{beta-D-GlcNAc-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[beta-D-GlcNAc-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc}-L-asparaginyl-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12941"/>
            <dbReference type="Rhea" id="RHEA-COMP:13526"/>
            <dbReference type="Rhea" id="RHEA-COMP:14369"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:60615"/>
            <dbReference type="ChEBI" id="CHEBI:60651"/>
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        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 24">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyltransferase 16 (GT16) protein family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.143" evidence="5"/>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000031054"/>
    <dbReference type="Ensembl" id="ENSCINT00000035709.1">
        <property type="protein sequence ID" value="ENSCINP00000031054.1"/>
        <property type="gene ID" value="ENSCING00000018255.1"/>
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    <dbReference type="GeneTree" id="ENSGT00390000007341"/>
    <dbReference type="HOGENOM" id="CLU_032753_1_0_1"/>
    <dbReference type="InParanoid" id="H2XN21"/>
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    <dbReference type="UniPathway" id="UPA00378"/>
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        <property type="component" value="Chromosome 4"/>
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        <property type="term" value="C:Golgi membrane"/>
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        <property type="term" value="C:Golgi stack"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:alpha-1,6-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="F:metal ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:oligosaccharide biosynthetic process"/>
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        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
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    </dbReference>
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        <property type="entry name" value="GlcNAc_II"/>
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    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
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    <dbReference type="PANTHER" id="PTHR12871:SF0">
        <property type="entry name" value="ALPHA-1,6-MANNOSYL-GLYCOPROTEIN 2-BETA-N-ACETYLGLUCOSAMINYLTRANSFERASE"/>
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    </dbReference>
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        <property type="entry name" value="BETA-1,2-N-ACETYLGLUCOSAMINYLTRANSFERASE II"/>
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        <property type="entry name" value="MGAT2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 25" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0328">Glycosyltransferase</keyword>
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    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 24" id="KW-0479">Metal-binding</keyword>
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    <keyword evidence="26" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="26">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003578033" description="Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase" evidence="26">
        <location>
            <begin position="25"/>
            <end position="423"/>
        </location>
    </feature>
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        <location>
            <begin position="92"/>
            <end position="96"/>
        </location>
        <ligand>
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        <location>
            <position position="123"/>
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        <ligand>
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        <location>
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        <ligand>
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        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
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        <location>
            <position position="343"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
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        <location>
            <begin position="165"/>
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        <location>
            <begin position="252"/>
            <end position="255"/>
        </location>
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        <location>
            <begin position="347"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00011011"/>
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            <dbReference type="ARBA" id="ARBA00012613"/>
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            <dbReference type="ARBA" id="ARBA00014817"/>
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            <dbReference type="ARBA" id="ARBA00022676"/>
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    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="423" mass="48479" checksum="DD219D41610D1175" modified="2012-04-18" version="1">KFPTHIKLPFVMVKFLFECGCSSAFNNKNMTWSSCFINFPSFKCISSPQLTFNFRLLKVLRNKAISMNFDQEMKNVAKFGMHAPDSVIFLLQVHSRPEFVQQLINSLRASTGIEHATVVISVDVDSPEINEVLARIDFCRYMVIFFPFAKVLYPNSFPGEDPNDCPKKIPKAQALERKCNNAEYPDMFGNYREVDFVQIKHHWFWKLNMAFSGIRAFNNQKAPIILLEDDYFVVPDIIHCAKQAEELRKSKCPRCQTISLGNYEQTQDYQAQGNQVEIKSWISSKHNLGMVVSSDFYNKIANCAEQFCNYDDYNWDWSLQAVSPHCEMGNLFTLAFKATRVFHLGSCGVHSKGECNAKQSAESRTAQLQNQKLYPESLHIAVDSPTVVPAPRPNGGWGDLRDQTLCKKYKHDNYRFCSSSCSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>H2XNH6</accession>
    <accession>A0A1W5BJN9</accession>
    <name>H2XNH6_CIOIN</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized LOC100185340</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">LOC100185340</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">wild type</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_018814862.2"/>
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        <property type="protein sequence ID" value="ENSCINP00000031209.1"/>
        <property type="gene ID" value="ENSCING00000024941.1"/>
    </dbReference>
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    <dbReference type="KEGG" id="cin:100185340"/>
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    <dbReference type="InParanoid" id="H2XNH6"/>
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        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.3980">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000031209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="109" mass="12435" checksum="E52848A9458456D5" modified="2012-04-18" version="1">MLGCCGSLLRIVSNVQALSACRSFYVSTLPSATVKNLQPSFSRRCENMLSPSNILFQPERNTTRGMNFQPNTYKRIKKHGRQKMLSSVNGKITMIRRMMKGKPRRTLTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>H2XPW8</accession>
    <name>H2XPW8_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ZP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
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                <person name="Kawashima T."/>
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                <person name="Terry A."/>
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                <person name="Oishi K."/>
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                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Mount S."/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
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                <person name="Kawashima T."/>
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                <person name="Putnam N."/>
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                <person name="Wang H.G."/>
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                <person name="Satou Y."/>
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                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
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                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
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                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
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                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
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                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
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                <person name="Suzuki M.M."/>
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                <person name="Wada S."/>
                <person name="Zhang C."/>
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                <person name="Larimer F."/>
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                <person name="Doggett N."/>
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                <person name="Kohara Y."/>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
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            <authorList>
                <consortium name="Ensembl"/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="De Tomaso A."/>
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                <person name="Di Gregorio A."/>
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                <person name="Hastings K.E."/>
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                <person name="Meinertzhagen I.A."/>
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                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
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                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
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                <person name="Levine M."/>
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    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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            <authorList>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
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                <person name="Huang W."/>
                <person name="Kawashima T."/>
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                <person name="Meinertzhagen I.A."/>
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                <person name="Nonaka M."/>
                <person name="Putnam N."/>
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                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
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                <person name="Azumi K."/>
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                <person name="Hyatt P.D."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
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                <person name="Chapman J."/>
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                <person name="De Tomaso A."/>
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                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
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                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
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                <person name="Tassy O."/>
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                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
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                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
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                <person name="Richardson P."/>
                <person name="Lucas S."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
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            <authorList>
                <consortium name="Ensembl"/>
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        <text evidence="1 7">Belongs to the universal ribosomal protein uS5 family.</text>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Mochizuki Y."/>
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                <person name="Tassy O."/>
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                <person name="Richardson P."/>
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                <person name="Levine M."/>
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                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="H2XXQ4"/>
    <dbReference type="Ensembl" id="ENSCINT00000030564.1">
        <property type="protein sequence ID" value="ENSCINP00000034438.1"/>
        <property type="gene ID" value="ENSCING00000024320.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00660000097124"/>
    <dbReference type="HOGENOM" id="CLU_2579906_0_0_1"/>
    <dbReference type="InParanoid" id="H2XXQ4"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000175">
        <property type="entry name" value="Na/ntran_symport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037272">
        <property type="entry name" value="SNS_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00209">
        <property type="entry name" value="SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161070">
        <property type="entry name" value="SNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="22"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000034438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="81" mass="9454" checksum="F8C64B29C93DF4D6" modified="2012-04-18" version="1">YCIKWEPLKVGDYVYPWWANGIGWILSLSSILWIPGVACYVMARGEGTLSQRFKNGIKWDLEEKYNPGSDEDKEERIELKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>H2XXX1</accession>
    <name>H2XXX1_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nuclear cap-binding protein subunit 3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">wild type</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NCBP3 family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01000722">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XXX1"/>
    <dbReference type="STRING" id="7719.ENSCINP00000034505"/>
    <dbReference type="Ensembl" id="ENSCINT00000034404.1">
        <property type="protein sequence ID" value="ENSCINP00000034505.1"/>
        <property type="gene ID" value="ENSCING00000024679.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000005712"/>
    <dbReference type="HOGENOM" id="CLU_1647527_0_0_1"/>
    <dbReference type="InParanoid" id="H2XXX1"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000340">
        <property type="term" value="F:RNA 7-methylguanosine cap binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019416">
        <property type="entry name" value="NCBP3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16291">
        <property type="entry name" value="NUCLEAR CAP-BINDING PROTEIN SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16291:SF0">
        <property type="entry name" value="NUCLEAR CAP-BINDING PROTEIN SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="56"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="67"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="81"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="95"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000034505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="161" mass="18411" checksum="CB7AB9E2CD5A085B" modified="2012-04-18" version="1">MTLEIRYAELRDRKVLGAAKRSKYYINHGNPNYGGTKGLLSSAFRKRYQMSHARNELMNMNVPITNSRRDNNDDSGDSNHYRGKWSRRNKMRSDDVEEEDGKSKEELEDDQLMKSSAKDLTDAIPVNDSGDDSDGFGTMVADQVAKTVKEAREMKERGNFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>H2XY07</accession>
    <name>H2XY07_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Sodium/potassium-transporting ATPase subunit alpha</fullName>
        </recommendedName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="19">This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.</text>
    </comment>
    <comment type="subunit">
        <text evidence="20">The sodium/potassium-transporting ATPase is composed of a catalytic alpha subunit, an auxiliary non-catalytic beta subunit and an additional regulatory subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 21">Cell membrane</location>
            <topology evidence="1 21">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 21">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="H2XY07"/>
    <dbReference type="FunCoup" id="H2XY07">
        <property type="interactions" value="48"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000034541"/>
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</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
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        <name type="synonym">Ascidia intestinalis</name>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
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            <taxon>Ciona</taxon>
        </lineage>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">wild type</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000034765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
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    </evidence>
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<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
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        <name type="common">Transparent sea squirt</name>
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    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">wild type</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001497">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XZB7"/>
    <dbReference type="Ensembl" id="ENSCINT00000030506.1">
        <property type="protein sequence ID" value="ENSCINP00000035001.1"/>
        <property type="gene ID" value="ENSCING00000021354.1"/>
    </dbReference>
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    <dbReference type="InParanoid" id="H2XZB7"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
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        <source>
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        <source>
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        <source>
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        <source>
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            <dbReference type="Pfam" id="PF07533"/>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000035001.1"/>
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        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
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                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
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    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
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                <person name="Wang H.G."/>
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                <person name="Azumi K."/>
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                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
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                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
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                <person name="Mount S."/>
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                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
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                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="H2XZF0"/>
    <dbReference type="Ensembl" id="ENSCINT00000035586.1">
        <property type="protein sequence ID" value="ENSCINP00000035034.1"/>
        <property type="gene ID" value="ENSCING00000023804.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00660000097434"/>
    <dbReference type="HOGENOM" id="CLU_2072297_0_0_1"/>
    <dbReference type="InParanoid" id="H2XZF0"/>
    <dbReference type="OMA" id="STYMAVC"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000035034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="118" mass="13473" checksum="15A31153CC388B23" modified="2012-04-18" version="1">MLQGFVQTDAVGKILFPNIHNVFVVNYKEYVRSIPWQHNNITGDTFRQFNGCTMLICGFLLVLNKYVKVSSFVMHVMALWSVYAHMLASDSTYMAVCGGVISTLMLFMCFAQKKEKED</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>H2XZH4</accession>
    <accession>A0A1W2W9E8</accession>
    <name>H2XZH4_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Regulator of G-protein signaling 18</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC100184282</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01000814">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002127275.1">
        <property type="nucleotide sequence ID" value="XM_002127239.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XZH4"/>
    <dbReference type="STRING" id="7719.ENSCINP00000035058"/>
    <dbReference type="Ensembl" id="ENSCINT00000035214.1">
        <property type="protein sequence ID" value="ENSCINP00000035058.1"/>
        <property type="gene ID" value="ENSCING00000023631.1"/>
    </dbReference>
    <dbReference type="GeneID" id="100184282"/>
    <dbReference type="KEGG" id="cin:100184282"/>
    <dbReference type="GeneTree" id="ENSGT00940000154416"/>
    <dbReference type="HOGENOM" id="CLU_897023_0_0_1"/>
    <dbReference type="InParanoid" id="H2XZH4"/>
    <dbReference type="OMA" id="SPIPMTK"/>
    <dbReference type="OrthoDB" id="196547at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="CDD" id="cd07440">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.167.10:FF:000001">
        <property type="entry name" value="Putative regulator of g-protein signaling 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.167.10">
        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016137">
        <property type="entry name" value="RGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036305">
        <property type="entry name" value="RGS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044926">
        <property type="entry name" value="RGS_subdomain_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10845">
        <property type="entry name" value="REGULATOR OF G PROTEIN SIGNALING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10845:SF259">
        <property type="entry name" value="RGS DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00615">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RGSPROTEIN"/>
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        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48097">
        <property type="entry name" value="Regulator of G-protein signaling, RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50132">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="201"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000035058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="310" mass="35991" checksum="2568DBDCECC6AC01" modified="2012-04-18" version="1">MSCLDITTTAVVPDRIASRYRRRSMTSFECDVRQSTSSYEGKLSISSDGFRTMMRDIENGLSRRSKQNCSSTSDLNGFEFGGFIAQDSPFTKVKKTRRVQNIGKAGKRKYFGSISNISVCSSVSYRVKRLDVSTSRLKERMLKKSNRKSSDKNEKLQQKLDNLFAWLQRSGKKAGRYALNRRGSPIPMTKLETRKKSLDLTLKEVLSKKETTNAFRKFLSREMSEENLDFWLEVESYKKAKESKRQKLATRIYEKYISPSSTHEINLESYVRDHTERKLKDVSPDTFDLAQKHIFALMETDSFRRFLDLF</sequence>
</entry>
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    <accession>H2Y015</accession>
    <name>H2Y015_CIOIN</name>
    <protein>
        <recommendedName>
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    <organism evidence="3 4">
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        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
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            <taxon>Phlebobranchia</taxon>
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    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="H2Y015"/>
    <dbReference type="STRING" id="7719.ENSCINP00000035249"/>
    <dbReference type="Ensembl" id="ENSCINT00000034248.1">
        <property type="protein sequence ID" value="ENSCINP00000035249.1"/>
        <property type="gene ID" value="ENSCING00000022280.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000167696"/>
    <dbReference type="HOGENOM" id="CLU_110070_0_0_1"/>
    <dbReference type="InParanoid" id="H2Y015"/>
    <dbReference type="OMA" id="CKGKMFC"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
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    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.300.10">
        <property type="entry name" value="Tie2 ligand-binding domain superfamily"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052108">
        <property type="entry name" value="MEGF/SIB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24035">
        <property type="entry name" value="MULTIPLE EPIDERMAL GROWTH FACTOR-LIKE DOMAINS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24035:SF109">
        <property type="entry name" value="PROTEIN DRAPER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="1" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="102"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="128"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000035249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="182" mass="20074" checksum="EE0E9BC5AD369DB4" modified="2012-04-18" version="1">MSKNALIFPTSLSYRASINELITLNMIQAQRMPIDELVWYHLLNYASPRRLAVGQLQLNIQSAKREDSGPYLIFFPVNNPIRRVLLQALTRVVVRNCIADMFGENCDQVCPSCENGGICDDVSGNCICPPGFMGELCQIGCGPNKFGRRCQYLCSEDPGADQDAGCKGKMFCLADPYGCSCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>H2Y038</accession>
    <name>H2Y038_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF4371 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Ciona intestinalis</name>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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        <citation type="journal article" date="2003" name="Dev. Genes Evol." volume="213" first="211" last="212">
            <title>Genomewide surveys of developmentally relevant genes in Ciona intestinalis.</title>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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                <person name="Yagi K."/>
                <person name="Satoh N."/>
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            <dbReference type="DOI" id="10.1242/dev.01270"/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <citation type="submission" date="2005-04" db="EMBL/GenBank/DDBJ databases">
            <title>Expressed genes in Ciona intestinalis.</title>
            <authorList>
                <person name="Satou Y."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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    <reference evidence="9" key="5">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <citation type="journal article" date="2003" name="Dev. Genes Evol." volume="213" first="211" last="212">
            <title>Genomewide surveys of developmentally relevant genes in Ciona intestinalis.</title>
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                <person name="Satoh N."/>
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            <dbReference type="DOI" id="10.1007/s00427-003-0330-z"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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    <reference evidence="2" key="3">
        <citation type="journal article" date="2004" name="Development" volume="131" first="4047" last="4058">
            <title>Gene expression profiles of transcription factors and signaling molecules in the ascidian embryo: towards a comprehensive understanding of gene networks.</title>
            <authorList>
                <person name="Imai K.S."/>
                <person name="Hino K."/>
                <person name="Yagi K."/>
                <person name="Satoh N."/>
                <person name="Satou Y."/>
            </authorList>
            <dbReference type="PubMed" id="15269171"/>
            <dbReference type="DOI" id="10.1242/dev.01270"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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    <reference evidence="2" key="4">
        <citation type="submission" date="2005-04" db="EMBL/GenBank/DDBJ databases">
            <title>Expressed genes in Ciona intestinalis.</title>
            <authorList>
                <person name="Satou Y."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="3" key="5">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">wild type</strain>
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        <citation type="submission" date="2025-05" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="16" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">wild type</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Catalytic subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment. In aerobic conditions, involved in intracellular iron homeostasis, thus triggering the activity of Fe(2+) prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation. May play a role in neurite development and synaptic connectivity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + H2O + 4 H(+)(in) = ADP + phosphate + 5 H(+)(out)</text>
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            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.1.2.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
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        <property type="nucleotide sequence ID" value="XM_002130711.5"/>
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        <property type="nucleotide sequence ID" value="XM_009860091.3"/>
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        <property type="interactions" value="567"/>
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        <property type="protein sequence ID" value="ENSCINP00000029917.1"/>
        <property type="gene ID" value="ENSCING00000023010.1"/>
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    <dbReference type="GeneID" id="100184809"/>
    <dbReference type="KEGG" id="cin:100184809"/>
    <dbReference type="eggNOG" id="KOG1352">
        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="OMA" id="RIVKTFW"/>
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    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 4"/>
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    <dbReference type="GO" id="GO:0033180">
        <property type="term" value="C:proton-transporting V-type ATPase, V1 domain"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:ATP binding"/>
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        <property type="term" value="F:ATP hydrolysis activity"/>
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        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
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        <property type="term" value="P:ATP metabolic process"/>
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        <property type="term" value="P:homeostatic process"/>
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    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18111">
        <property type="entry name" value="ATP-synt_V_A-type_alpha_C"/>
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    </dbReference>
    <dbReference type="CDD" id="cd18119">
        <property type="entry name" value="ATP-synt_V_A-type_alpha_N"/>
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    </dbReference>
    <dbReference type="CDD" id="cd01134">
        <property type="entry name" value="V_A-ATPase_A"/>
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    </dbReference>
    <dbReference type="FunFam" id="1.10.1140.10:FF:000002">
        <property type="entry name" value="V-type proton ATPase catalytic subunit A"/>
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    </dbReference>
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    <dbReference type="Gene3D" id="1.10.1140.10">
        <property type="entry name" value="Bovine Mitochondrial F1-atpase, Atp Synthase Beta Chain, Chain D, domain 3"/>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ATP_synth_A_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ATP-synt_VA_C"/>
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        <property type="entry name" value="ATP-synth_a_Xtn"/>
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        <property type="entry name" value="ATP_synth_asu-like_sf"/>
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        <property type="entry name" value="ATPase_a/bsu_AS"/>
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        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
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    <dbReference type="InterPro" id="IPR036121">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
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        <property type="entry name" value="ATPase_F1/V1_b/a_C"/>
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        <property type="entry name" value="ATPase_V1-cplx_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022878">
        <property type="entry name" value="V-ATPase_asu"/>
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        <property type="entry name" value="PRK04192.1"/>
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        <property type="entry name" value="V-ATPase_V1_A"/>
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    <dbReference type="PANTHER" id="PTHR43607:SF1">
        <property type="entry name" value="H(+)-TRANSPORTING TWO-SECTOR ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43607">
        <property type="entry name" value="V-TYPE PROTON ATPASE CATALYTIC SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16886">
        <property type="entry name" value="ATP-synt_ab_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22919">
        <property type="entry name" value="ATP-synt_VA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47917">
        <property type="entry name" value="C-terminal domain of alpha and beta subunits of F1 ATP synthase"/>
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    </dbReference>
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        <property type="entry name" value="N-terminal domain of alpha and beta subunits of F1 ATP synthase"/>
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        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
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    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-1278">Translocase</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
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        <location>
            <begin position="24"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="ATPsynthase alpha/beta subunit barrel-sandwich" evidence="13">
        <location>
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            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="ATPase F1/V1/A1 complex alpha/beta subunit nucleotide-binding" evidence="11">
        <location>
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        </location>
    </feature>
    <feature type="domain" description="ATP synthase A/B type C-terminal" evidence="14">
        <location>
            <begin position="465"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008936"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012473"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00046187"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
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    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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</entry>
<entry dataset="TrEMBL" created="2017-06-07" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1W3JEV5</accession>
    <accession>F6SQQ1</accession>
    <name>A0A1W3JEV5_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Eukaryotic translation initiation factor 6</fullName>
            <shortName evidence="6">eIF-6</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100178387</name>
        <name evidence="6" type="synonym">EIF6</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">wild type</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm. May also be involved in ribosome biogenesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Monomer. Associates with the 60S ribosomal subunit. Interacts with RACK1. Interacts with DICER1, AGO2, TARBP2, MOV10 and RPL7A; they form a large RNA-induced silencing complex (RISC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
            <location evidence="6">Nucleolus</location>
        </subcellularLocation>
        <text evidence="6">Shuttles between cytoplasm and nucleus/nucleolus.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the eIF-6 family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002880">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_026691921.1">
        <property type="nucleotide sequence ID" value="XM_026836120.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1W3JEV5"/>
    <dbReference type="FunCoup" id="A0A1W3JEV5">
        <property type="interactions" value="853"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000025693"/>
    <dbReference type="Ensembl" id="ENSCINT00000025939.1">
        <property type="protein sequence ID" value="ENSCINP00000025693.1"/>
        <property type="gene ID" value="ENSCING00000014133.1"/>
    </dbReference>
    <dbReference type="GeneID" id="100178387"/>
    <dbReference type="KEGG" id="cin:100178387"/>
    <dbReference type="eggNOG" id="KOG3185">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015972"/>
    <dbReference type="InParanoid" id="A0A1W3JEV5"/>
    <dbReference type="OMA" id="WCAFCGM"/>
    <dbReference type="OrthoDB" id="4155914at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902626">
        <property type="term" value="P:assembly of large subunit precursor of preribosome"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0042256">
        <property type="term" value="P:cytosolic ribosome assembly"/>
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    <dbReference type="GO" id="GO:0000460">
        <property type="term" value="P:maturation of 5.8S rRNA"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0000470">
        <property type="term" value="P:maturation of LSU-rRNA"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000054">
        <property type="term" value="P:ribosomal subunit export from nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00527">
        <property type="entry name" value="IF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.75.10.10:FF:000001">
        <property type="entry name" value="Eukaryotic translation initiation factor 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.75.10.10">
        <property type="entry name" value="L-arginine/glycine Amidinotransferase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00032">
        <property type="entry name" value="eIF_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002769">
        <property type="entry name" value="eIF6"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00323">
        <property type="entry name" value="eIF-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10784">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01912">
        <property type="entry name" value="eIF-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006413">
        <property type="entry name" value="IF-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00654">
        <property type="entry name" value="eIF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55909">
        <property type="entry name" value="Pentein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 6" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="4 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3 6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0690">Ribosome biogenesis</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00062592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000025693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="245" mass="26796" checksum="7BAA25985901A153" modified="2017-06-07" version="1">MAVRAQFENNNEVGVFSKLTNRYCLVAIGGSENFYSVFESELADVIPVVHASIGGCRIIGRMCVGNRNGLMVPNSTTDQELQHIRNCLPDEVKIQRVEERLSALGNVIACNDYVALVHPDLDRETEEILADVLKVEVFRQTVTDQVLVGSYCCFSNKGGLVHPKTTIEDQDELSSLIQVPLVAGTVNRGSEVIGGGMVVNDWCAFCGLDTTSTEISVIESVFNLGEQHSSAIKNRMRDSLIDAMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-07-07" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>C4B8X0</accession>
    <accession>A0A1W2VYS5</accession>
    <accession>F6RKS5</accession>
    <name>C4B8X0_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="9 10">Radial spoke protein NDK/DM44</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">Ci-NDK/DM44</name>
        <name evidence="10" type="synonym">ci-ndk/dm44</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">wild type</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="journal article" date="2009" name="FEBS Lett." volume="583" first="2201" last="2207">
            <title>Proteomic characterization of sperm radial spokes identifies a novel spoke protein with an ubiquitin domain.</title>
            <authorList>
                <person name="Satouh Y."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="19527718"/>
            <dbReference type="DOI" id="10.1016/j.febslet.2009.06.016"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Testis</tissue>
        </source>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 7">Belongs to the NDK family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01000095">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AB501294">
        <property type="protein sequence ID" value="BAH59279.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001155162.1">
        <property type="nucleotide sequence ID" value="NM_001161690.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C4B8X0"/>
    <dbReference type="IntAct" id="C4B8X0">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="MINT" id="C4B8X0"/>
    <dbReference type="STRING" id="7719.ENSCINP00000025129"/>
    <dbReference type="Ensembl" id="ENSCINT00000025375.3">
        <property type="protein sequence ID" value="ENSCINP00000025129.3"/>
        <property type="gene ID" value="ENSCING00000013757.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100176380"/>
    <dbReference type="KEGG" id="cin:100176380"/>
    <dbReference type="CTD" id="100176380"/>
    <dbReference type="GeneTree" id="ENSGT00940000158946"/>
    <dbReference type="HOGENOM" id="CLU_060216_3_1_1"/>
    <dbReference type="OMA" id="VCMCLEI"/>
    <dbReference type="OrthoDB" id="270127at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005879">
        <property type="term" value="C:axonemal microtubule"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004550">
        <property type="term" value="F:nucleoside diphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006241">
        <property type="term" value="P:CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006183">
        <property type="term" value="P:GTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006228">
        <property type="term" value="P:UTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04412">
        <property type="entry name" value="NDPk7B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.141:FF:000004">
        <property type="entry name" value="Nucleoside diphosphate kinase 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.141">
        <property type="entry name" value="Nucleoside diphosphate kinase-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006602">
        <property type="entry name" value="DM10_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057579">
        <property type="entry name" value="DM10_NDK7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034907">
        <property type="entry name" value="NDK-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036850">
        <property type="entry name" value="NDK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011410">
        <property type="entry name" value="NDPK7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037993">
        <property type="entry name" value="NDPk7B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001564">
        <property type="entry name" value="Nucleoside_diP_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43109">
        <property type="entry name" value="NUCLEOSIDE DIPHOSPHATE KINASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43109:SF2">
        <property type="entry name" value="NUCLEOSIDE DIPHOSPHATE KINASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00334">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25364">
        <property type="entry name" value="PH_NDK7_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036503">
        <property type="entry name" value="NDK7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01243">
        <property type="entry name" value="NUCDPKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00676">
        <property type="entry name" value="DM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00562">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54919">
        <property type="entry name" value="Nucleoside diphosphate kinase, NDK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51336">
        <property type="entry name" value="DM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51374">
        <property type="entry name" value="NDPK_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-0966">Cell projection</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DM10" evidence="8">
        <location>
            <begin position="16"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="active site" description="Pros-phosphohistidine intermediate" evidence="5">
        <location>
            <position position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR036503-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAH59279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000025129.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="388" mass="43646" checksum="62D8D2BDAE91A12C" modified="2009-07-07" version="1">MFPGYSDAYIEPSSAQDERYSFIAEWYDPQAALIRRYQFLYYPKDSSIEMFDIKNRRKFLSRTKFESVRLEDLYIGSKVSIYSRQLTFVDFGDQFTESKLISKKEKTFALIKPDATSKLGVILNGLRDRNIKVTKAQMVQMTRTDAVKFYDEHQSKPYFNSILEYITSGPVIAMEIVGSGVVQKWLEMLGPSDPSEARTSNPKSIRAMFGTDELKNTAHGSINATAAAKELEFFFPSSGAAFVGTAKYENSTCCVIKPHAVKEGLVGDIISVIMDAGFDITALSMQTVQKANAEEFYEIYKGVVAEYKSMVDELCNGSCVVLEISGNDQDVPSKFRQLCGPSDPEIGRHLRPKTLRAMFGKDAIQNAVHCTDLPEDGILEVEYFFRIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>F6PIN4</accession>
    <name>F6PIN4_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
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            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">wild type</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01001004">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6PIN4"/>
    <dbReference type="Ensembl" id="ENSCINT00000020222.3">
        <property type="protein sequence ID" value="ENSCINP00000020222.3"/>
        <property type="gene ID" value="ENSCING00000010102.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000067374"/>
    <dbReference type="HOGENOM" id="CLU_1460800_0_0_1"/>
    <dbReference type="InParanoid" id="F6PIN4"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000020222.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="185" mass="21194" checksum="A9511B0715A93013" modified="2011-07-27" version="1">MEPEVLKLPTKQREELYTDIARSEVSKKDVNPIAKKNVVFTEKENSSDTTSVPDIGTQSHNLGFMYGYRNAALQCLLHISTKLLASFKTEKAPVPKVRDLWMLSLDNCQKRRKFNNQWQSERISEKENLIVASTNTEHALPSADIVSASRSSTYTMNEESMRAFFNMEHALTPIKTPLYYETSER</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>F6PUL6</accession>
    <name>F6PUL6_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Helicase ATP-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
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            <taxon>Metazoa</taxon>
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            <taxon>Tunicata</taxon>
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            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01000233">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6PUL6"/>
    <dbReference type="STRING" id="7719.ENSCINP00000023858"/>
    <dbReference type="Ensembl" id="ENSCINT00000024104.2">
        <property type="protein sequence ID" value="ENSCINP00000023858.2"/>
        <property type="gene ID" value="ENSCING00000012878.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153173"/>
    <dbReference type="HOGENOM" id="CLU_670756_0_0_1"/>
    <dbReference type="InParanoid" id="F6PUL6"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006935">
        <property type="entry name" value="Helicase/UvrB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051363">
        <property type="entry name" value="RLR_Helicase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14074:SF16">
        <property type="entry name" value="ANTIVIRAL INNATE IMMUNE RESPONSE RECEPTOR RIG-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14074">
        <property type="entry name" value="HELICASE WITH DEATH DOMAIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04851">
        <property type="entry name" value="ResIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="1">
        <location>
            <begin position="308"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000023858.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="410" mass="47442" checksum="218538E36C1CEB8F" modified="2012-04-18" version="2">MKKEQKDFLLHWENLFKQFLIPQKFASAFPEWDIWDRWSNENEETKVQIFLDKLRDSEESGIFKRFIDNLRHCSEQKWIFDLIDGKLDPKEQIKCFKHVVVAPQLKTLFKLLEKDYEIVDARSMIAEFTAYFTTAEKDWLLAIDRDRGNAPAMTAFFSIMTRKHPKWLKEFYDSLNENKYHHPDSPTLAESFQQYIKLEEKRDGEASIAVTEDNDQTVPEPMDQACALPPVEVIQEDNEECGTSFLLCMDLLNNLSPFEEDCNSNNDSLNNFNVMAVEEAMETCTSEDVCQEESLPELNLRDYQLELAEQAMSGENSLIVAPTGSGKTFVALKVCKDHIEKRPDSRVVFMVPRVPLIKQQYELFKCFLGDGQGILRAISTDETLSAPADRLILDHKISFLTPQILVNLLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>F6Q678</accession>
    <name>F6Q678_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcription factor protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC778714</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_026694096.1">
        <property type="nucleotide sequence ID" value="XM_026838295.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6Q678"/>
    <dbReference type="STRING" id="7719.ENSCINP00000018171"/>
    <dbReference type="Ensembl" id="ENSCINT00000018171.3">
        <property type="protein sequence ID" value="ENSCINP00000018171.3"/>
        <property type="gene ID" value="ENSCING00000015262.2"/>
    </dbReference>
    <dbReference type="GeneID" id="778714"/>
    <dbReference type="GeneTree" id="ENSGT00940000168146"/>
    <dbReference type="HOGENOM" id="CLU_604027_0_0_1"/>
    <dbReference type="InParanoid" id="F6Q678"/>
    <dbReference type="OMA" id="SAICKWI"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001766">
        <property type="entry name" value="Fork_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047513">
        <property type="entry name" value="FOXJ1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46805">
        <property type="entry name" value="FORKHEAD BOX PROTEIN J1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46805:SF1">
        <property type="entry name" value="FORKHEAD BOX PROTEIN J1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00250">
        <property type="entry name" value="Forkhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00053">
        <property type="entry name" value="FORKHEAD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00339">
        <property type="entry name" value="FH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50039">
        <property type="entry name" value="FORK_HEAD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fork-head" evidence="4">
        <location>
            <begin position="129"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Fork-head" evidence="2">
        <location>
            <begin position="129"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="63"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="240"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="110"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="240"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000018171.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="453" mass="52025" checksum="712B3D6055D9D299" modified="2012-04-18" version="2">MNNAVRKKLRRSEIHERLKQNYEALYARCPPLGPNKEMLDDSLTNLQWLWNLNVSMTFPIEINKGEKNGKPAKQQARQEHEASPSKPQCEKYFNQSQRRKISQTTWKQTKQIEKREKHPVMGNGSKFEKPAYSVAQLICMAIENSKHKKLSLSAICKWIRDNFAYYRNIEESLQNSVRLCLVGNGYFQKSSPNEASNKYLWEIKPKIYFGRPIFSQGGSKDMTPPNLPITNVDFLDSASNRKRKQVAPQKVIRRKNTKKRRNINENSSPVSPNLLEEEAPELGSLKGDFNWNSILDGLFPTSPGDLSLSANEEKVCNEFLTKHDGSMFAHNGFNDNVNKENGDNGLLPHLHEQCEDGYFPFTDTSTEETKYFKEFLDEKNELDLTVTGHQIEIPSEWEPVTSTLNDILCEKQDDNVAGFSQTQAIQQTDKTNHPWQEVENATDVIEDILMSTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>F6QE85</accession>
    <name>F6QE85_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Serpin A3-5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100177185</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">wild type</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the serpin family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01000093">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6QE85"/>
    <dbReference type="Ensembl" id="ENSCINT00000018441.3">
        <property type="protein sequence ID" value="ENSCINP00000018441.3"/>
        <property type="gene ID" value="ENSCING00000009089.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163730"/>
    <dbReference type="HOGENOM" id="CLU_023330_0_1_1"/>
    <dbReference type="InParanoid" id="F6QE85"/>
    <dbReference type="OMA" id="FQADWAI"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00172">
        <property type="entry name" value="serpin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.39.10">
        <property type="entry name" value="Alpha-1-antitrypsin, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.497.10">
        <property type="entry name" value="Antithrombin, subunit I, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.580">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.310.10">
        <property type="entry name" value="Serpins superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023795">
        <property type="entry name" value="Serpin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023796">
        <property type="entry name" value="Serpin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000215">
        <property type="entry name" value="Serpin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036186">
        <property type="entry name" value="Serpin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042178">
        <property type="entry name" value="Serpin_sf_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042185">
        <property type="entry name" value="Serpin_sf_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461:SF211">
        <property type="entry name" value="GH10112P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461">
        <property type="entry name" value="SERINE PROTEASE INHIBITOR, SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00079">
        <property type="entry name" value="Serpin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00093">
        <property type="entry name" value="SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56574">
        <property type="entry name" value="Serpins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00284">
        <property type="entry name" value="SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003344789" evidence="3">
        <location>
            <begin position="19"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="domain" description="Serpin" evidence="4">
        <location>
            <begin position="36"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000018441.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="400" mass="45523" checksum="19AD5B92531DE8DF" modified="2012-04-18" version="2">MKLLICSLLLLVIATGYCQNRRWINHFTDNQNTFSGSLYWAISKEKPNKNVLFSPISVSQTLGMVLAGAMGNTYDEITRALQMTDLTPSRIHTLMRKTRNNVVMRPNGQTVKLANSVFIGSNYPVVQQYIDLLRQNYKSSVFPVNFHNSNAAANMINEWVSNMTEDKIRELVDPSSITAFTRMILVNAVYFQADWAISFRRIPTNKQNFFLSNGTTVQVPFMVRWEAVVKSYNYRDKIEFFFIRYKTTSNQNTYFVVGLPGDNYSESKEAQQILSRFRTINKMFRITHFKMPLIELSHKTDVKEVLQTLGVVDLFDSGASNLTGISTVEQLYVSEFTQKAYINVNENGTVAAAASAATVQGRSLSIPRQVTVDRPFFIGVYQEKSNSFLFLGKVENPLEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>F6QGX2</accession>
    <name>F6QGX2_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01000498">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6QGX2"/>
    <dbReference type="Ensembl" id="ENSCINT00000016922.3">
        <property type="protein sequence ID" value="ENSCINP00000016922.3"/>
        <property type="gene ID" value="ENSCING00000008286.3"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1849557_0_0_1"/>
    <dbReference type="InParanoid" id="F6QGX2"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="91"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="24"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000016922.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="139" mass="15349" checksum="569D27A5A605E93B" modified="2012-04-18" version="2">MRSSERRRKKELNHGPSYNRLHGAPPSARLRASASSPSFSGTRPRVRFDEGPHEVRTIDSQQQPKSTFVNFFGDHRAPSSDVELEEVMRHYGINDADSNNIPSSSPLPKPTPSNEHNQRLASVAVSVSSGLGNFLLAFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>F6QIF4</accession>
    <name>F6QIF4_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 14">Telomerase reverse transcriptase</fullName>
            <ecNumber evidence="2 14">2.7.7.49</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12 14">Telomerase catalytic subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC100187276</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. It elongates telomeres. It is a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.49"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Chromosome</location>
            <location evidence="14">Telomere</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 14">Belongs to the reverse transcriptase family. Telomerase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.49" evidence="2 14"/>
    <dbReference type="AlphaFoldDB" id="F6QIF4"/>
    <dbReference type="STRING" id="7719.ENSCINP00000018420"/>
    <dbReference type="Ensembl" id="ENSCINT00000018420.3">
        <property type="protein sequence ID" value="ENSCINP00000018420.3"/>
        <property type="gene ID" value="ENSCING00000013094.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000018531"/>
    <dbReference type="HOGENOM" id="CLU_320766_0_0_1"/>
    <dbReference type="InParanoid" id="F6QIF4"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000333">
        <property type="term" value="C:telomerase catalytic core complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003720">
        <property type="term" value="F:telomerase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070034">
        <property type="term" value="F:telomerase RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042162">
        <property type="term" value="F:telomeric DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007004">
        <property type="term" value="P:telomere maintenance via telomerase"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01648">
        <property type="entry name" value="TERT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021891">
        <property type="entry name" value="Telomerase_RBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003545">
        <property type="entry name" value="Telomerase_RT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12066">
        <property type="entry name" value="TELOMERASE REVERSE TRANSCRIPTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12066:SF0">
        <property type="entry name" value="TELOMERASE REVERSE TRANSCRIPTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12009">
        <property type="entry name" value="Telomerase_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01365">
        <property type="entry name" value="TELOMERASERT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00975">
        <property type="entry name" value="Telomerase_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 14" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8 14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 14" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 14" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="9 14" id="KW-0779">Telomere</keyword>
    <keyword evidence="5 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="16">
        <location>
            <begin position="420"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="198"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="208"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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                <person name="Sasakura Y."/>
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                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
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                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
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                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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            <authorList>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
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                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
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            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">wild type</strain>
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    <reference evidence="1" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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            <dbReference type="Ensembl" id="ENSCINP00000020047.3"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
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    <accession>F6R6C4</accession>
    <name>F6R6C4_CIOIN</name>
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        <recommendedName>
            <fullName evidence="9">U4/U6.U5 tri-snRNP-associated protein 1</fullName>
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    <organism evidence="7 8">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
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                <person name="Hastings K.E."/>
                <person name="Ho I."/>
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                <person name="Kawashima T."/>
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                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Yamada L."/>
                <person name="Wang H.G."/>
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                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
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                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="7" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
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                <person name="Matsumoto J."/>
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                <person name="Wiley G.B."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="7">wild type</strain>
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    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="7" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
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    <comment type="similarity">
        <text evidence="2">Belongs to the SNU66/SART1 family.</text>
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</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>F6RGA3</accession>
    <name>F6RGA3_CIOIN</name>
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        <recommendedName>
            <fullName evidence="10">C-type lectin domain-containing protein</fullName>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <citation type="submission" date="2025-09" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
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        </source>
    </evidence>
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        <source>
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        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>F6RXZ8</accession>
    <accession>A0A1W2WQH0</accession>
    <name>F6RXZ8_CIOIN</name>
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        <submittedName>
            <fullName evidence="14">Ras-related GTP-binding protein C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC100180665</name>
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    <organism evidence="14 15">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
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            <taxon>Ascidiacea</taxon>
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            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">wild type</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Lysosome membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the GTR/RAG GTP-binding protein family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001517">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002132063.1">
        <property type="nucleotide sequence ID" value="XM_002132027.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6RXZ8"/>
    <dbReference type="FunCoup" id="F6RXZ8">
        <property type="interactions" value="825"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000010644"/>
    <dbReference type="Ensembl" id="ENSCINT00000010644.3">
        <property type="protein sequence ID" value="ENSCINP00000010644.3"/>
        <property type="gene ID" value="ENSCING00000005166.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100180665"/>
    <dbReference type="KEGG" id="cin:100180665"/>
    <dbReference type="GeneTree" id="ENSGT00950000183031"/>
    <dbReference type="HOGENOM" id="CLU_047421_1_1_1"/>
    <dbReference type="InParanoid" id="F6RXZ8"/>
    <dbReference type="OMA" id="HEIFEVR"/>
    <dbReference type="OrthoDB" id="26136at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990131">
        <property type="term" value="C:Gtr1-Gtr2 GTPase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019899">
        <property type="term" value="F:enzyme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019003">
        <property type="term" value="F:GDP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034198">
        <property type="term" value="P:cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009267">
        <property type="term" value="P:cellular response to starvation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010507">
        <property type="term" value="P:negative regulation of autophagy"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043200">
        <property type="term" value="P:response to amino acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11385">
        <property type="entry name" value="RagC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.190:FF:000001">
        <property type="entry name" value="Ras-related GTP-binding protein C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000226">
        <property type="entry name" value="Ras-related GTP-binding protein D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006762">
        <property type="entry name" value="Gtr1_RagA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039400">
        <property type="entry name" value="RagC/D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11259:SF2">
        <property type="entry name" value="GH16429P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11259">
        <property type="entry name" value="RAS-RELATED GTP BINDING RAG/GTR YEAST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04670">
        <property type="entry name" value="Gtr1_RagA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 13" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0458">Lysosome</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU367014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000010644.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="371" mass="42005" checksum="FC54270FBE1190FE" modified="2011-07-27" version="1">MEPHEGFGLPVESFPKDFSYHGDDGEDAVATATSSDMKPRILIMGLQRSGKSSIQKVVFHKMSPNETLFLESTSKIFKDDVSNNSFINFQIWDIPGQIDFLDNTFDLDMIFGGTGALIFVVDAQDDYMEALTCLHQTVSKAFRVNPQIKFEIFIHKVDGLSDDHKIETQRDISQRANEELHEAGLRDLHLSFHLTSIYDHSIFEAFSKVVQKLIPQLTTLENLLSIFVQNSGVEKTFLFDVVSKIYIATDGSPVDMQSYELCCEMIDVMLDMTCIYGDETDDGTPQDSTCIIRLNNTSILYLQEVTRFLVLVCILRDDSFTRRGIIDYNILLLKKAINDVFDVRLRQGKQNGHLPIEGRNNLMQLSTSANA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>F6RZ45</accession>
    <name>F6RZ45_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">UV-stimulated scaffold protein A</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">wild type</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UVSSA family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01000248">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6RZ45"/>
    <dbReference type="FunCoup" id="F6RZ45">
        <property type="interactions" value="168"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000015212"/>
    <dbReference type="Ensembl" id="ENSCINT00000015212.3">
        <property type="protein sequence ID" value="ENSCINP00000015212.3"/>
        <property type="gene ID" value="ENSCING00000007407.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164256"/>
    <dbReference type="HOGENOM" id="CLU_023577_0_0_1"/>
    <dbReference type="InParanoid" id="F6RZ45"/>
    <dbReference type="OMA" id="EEHAEMR"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:response to UV"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006283">
        <property type="term" value="P:transcription-coupled nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018610">
        <property type="entry name" value="UVSSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049431">
        <property type="entry name" value="UVSSA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049408">
        <property type="entry name" value="UVSSA_N_a-solenoid_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28670">
        <property type="entry name" value="UV-STIMULATED SCAFFOLD PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28670:SF1">
        <property type="entry name" value="UV-STIMULATED SCAFFOLD PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09740">
        <property type="entry name" value="DUF2043"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20867">
        <property type="entry name" value="UVSSA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UV-stimulated scaffold protein A C-terminal" evidence="12">
        <location>
            <begin position="373"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="323"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="513"/>
            <end position="544"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF09740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000015212.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="591" mass="67022" checksum="4C6D0BD34E6C2668" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>F6S4X9</accession>
    <accession>A0A1W2WAR6</accession>
    <name>F6S4X9_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Xyloside xylosyltransferase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC100176443</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_002127967.1">
        <property type="nucleotide sequence ID" value="XM_002127931.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6S4X9"/>
    <dbReference type="FunCoup" id="F6S4X9">
        <property type="interactions" value="11"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000016452"/>
    <dbReference type="Ensembl" id="ENSCINT00000016452.3">
        <property type="protein sequence ID" value="ENSCINP00000016452.3"/>
        <property type="gene ID" value="ENSCING00000008046.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100176443"/>
    <dbReference type="KEGG" id="cin:100176443"/>
    <dbReference type="GeneTree" id="ENSGT00940000158154"/>
    <dbReference type="HOGENOM" id="CLU_048175_0_0_1"/>
    <dbReference type="InParanoid" id="F6S4X9"/>
    <dbReference type="OMA" id="NVWCIFT"/>
    <dbReference type="OrthoDB" id="411524at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140560">
        <property type="term" value="F:xylosyl alpha-1,3-xylosyltransferase activity"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016266">
        <property type="term" value="P:protein O-linked glycosylation via N-acetyl-galactosamine"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002495">
        <property type="entry name" value="Glyco_trans_8"/>
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        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042465">
        <property type="entry name" value="XXLT1"/>
    </dbReference>
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        <property type="entry name" value="XYLOSIDE XYLOSYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46612:SF1">
        <property type="entry name" value="XYLOSIDE XYLOSYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01501">
        <property type="entry name" value="Glyco_transf_8"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000016452.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="397" mass="46410" checksum="FE48CE52DF34733C" modified="2012-04-18" version="2">MAGQRGQNTQRRSRGQTNGLMSNLLSSKVMQVLLILCILSCVFLFYNFGTSTQTFTKESKAFYRAENHPINVKSDREEQIAGTFEGKQAKAAKDVAQKHADEFKNDYHVLFIFAHKNPKLEKKFTTAFGSMFKHAKFNERETFHLHFVCEKEGKEFVQQYFKEHVSHPKFNLKIHFHDTIKLARQVHPLVQGIQAMLGNDHPYFSETAIFFLSMVLPNIMPENIHRLVQLDLDLKFVTNIRDLWSEFDYFTKDTVIGIGNENQPVYRHLLWQYRNENPNTKVGEPYPAGQPGFNSGVVLLHLDNMRTSSLYNSYMNATNLQRIVQKYHFKGHLGDQDFYTLLAFEHPPLFHVLPCGWNKQLCQWWKDKGYQDIFDQYFDCKSEIKIWHGNCDSSFPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>F6S7P1</accession>
    <name>F6S7P1_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CALHM family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="F6S7P1"/>
    <dbReference type="Ensembl" id="ENSCINT00000027199.2">
        <property type="protein sequence ID" value="ENSCINP00000026953.2"/>
        <property type="gene ID" value="ENSCING00000015067.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234610"/>
    <dbReference type="HOGENOM" id="CLU_1274770_0_0_1"/>
    <dbReference type="InParanoid" id="F6S7P1"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005261">
        <property type="term" value="F:monoatomic cation channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904669">
        <property type="term" value="P:ATP export"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029569">
        <property type="entry name" value="CALHM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32261">
        <property type="entry name" value="CALCIUM HOMEOSTASIS MODULATOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32261:SF1">
        <property type="entry name" value="CALCIUM HOMEOSTASIS MODULATOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14798">
        <property type="entry name" value="Ca_hom_mod"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0407">Ion channel</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="30"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="138"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="162"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="217" mass="25181" checksum="7F22BB2CC180FB67" modified="2012-04-18" version="2">MTCRAVTRTPANKIRYTVTYERNKRKSQTLGWIFIALALFIGSIVYMANRWFSRLTYLHRNYMLWYHDIEDQKFELIAKEQIEEEAEKNIRNFLADKRPKLNWDKISTVFDLVRDRKGYAIYSRLDEWNECHPINVEDNISDKTTNGSKDKHSSVADDAGFGSSSEMKNNSEVVTSNSLIEPREIEMNTLSTGDTHKGYSPSAQHDIVSKPLLEFDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="Ensembl" id="ENSCINT00000024378.2">
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        <property type="gene ID" value="ENSCING00000013068.2"/>
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        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003346371" description="Secreted protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000024132.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="133" mass="15014" checksum="731F7A0DDD8F0856" modified="2012-04-18" version="2">MWVGRLWICHLLLLGLTDSNHILGSKADKEQRSSTCPIICQACAADHLKGYLQVKLPHDQMKGCSGRGMLEKRKSTDTEYVERHIIHVNNNQSETIYLSFHEMATASLLPPNSSSITIERKYKFALMSTKPIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>F6TGZ0</accession>
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        <submittedName>
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    <organism evidence="5 6">
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    <reference evidence="6" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
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                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
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                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">wild type</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Ensembl" id="ENSCINT00000016131.3">
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        <property type="gene ID" value="ENSCING00000007872.3"/>
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    <dbReference type="InParanoid" id="F6TGZ0"/>
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        <property type="component" value="Chromosome 7"/>
    </dbReference>
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        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ankyrin_rpt"/>
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    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
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    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="197"/>
            <end position="229"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
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            <begin position="11"/>
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        <location>
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            <end position="392"/>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
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        <source>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>F6TJD9</accession>
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        <submittedName>
            <fullName evidence="7">Transforming growth factor beta superfamily signaling ligand</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">tgfbeta-na4</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">wild type</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the TGF-beta family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001522">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6TJD9"/>
    <dbReference type="STRING" id="7719.ENSCINP00000021781"/>
    <dbReference type="Ensembl" id="ENSCINT00000022027.2">
        <property type="protein sequence ID" value="ENSCINP00000021781.2"/>
        <property type="gene ID" value="ENSCING00000009142.3"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_619557_0_0_1"/>
    <dbReference type="InParanoid" id="F6TJD9"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030509">
        <property type="term" value="P:BMP signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd13756">
        <property type="entry name" value="TGF_beta_BMPs_GDFs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001839">
        <property type="entry name" value="TGF-b_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015615">
        <property type="entry name" value="TGF-beta-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848">
        <property type="entry name" value="TGF-BETA FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848:SF119">
        <property type="entry name" value="TGF-BETA FAMILY PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00019">
        <property type="entry name" value="TGF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00204">
        <property type="entry name" value="TGFB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51362">
        <property type="entry name" value="TGF_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0339">Growth factor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003342548" evidence="5">
        <location>
            <begin position="17"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="domain" description="TGF-beta family profile" evidence="6">
        <location>
            <begin position="318"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000021781.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="442" mass="49778" checksum="5299E7F460CD98E4" modified="2011-07-27" version="1">MYCVVLAACFVATVAGINTRHGRTWYTTNRHEQSAIESCLRGQNMYVLVGSFATKCETLRVVARDMGNSDTLNRDRRVCRYFPSKGDSGFLELEYVKKLSDLKQRGQPSSEEALDNMLEAIAPCSVFSPSLRRKWKNFFDPRGGDRPIELITRFPVEHQTNTDHVVVSFTHPLRHNFSLHELIGVDLALLRIPPCQLNEGFGLYDVVVRKLPNDDSETEVVVHHSTQASCAGGGGYMIVASLDTGILMEWLLHEHSNLQLSVQVTQSRQVMSGNSSEPSVLNVLRRVLSLGFHLQAGPGASQQVIAKIPQGRRASHHRRRNSWITRMRRSPSRGIRGCERTSFPINFQDDWFRHHILYPSSVYDIGECVGRCDFPIQMPTNASNHALALTVEREFGLRSENEQPTICCVPTDYYSMYATLSSEFGVESVFLPEIEVKHCGCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>F6TX79</accession>
    <name>F6TX79_CIOIN</name>
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            <fullName evidence="3">SAM domain-containing protein</fullName>
        </recommendedName>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="F6TX79"/>
    <dbReference type="Ensembl" id="ENSCINT00000022550.2">
        <property type="protein sequence ID" value="ENSCINP00000022304.2"/>
        <property type="gene ID" value="ENSCING00000011735.2"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1182459_0_0_1"/>
    <dbReference type="InParanoid" id="F6TX79"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000022304.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="235" mass="26919" checksum="B15976EA38F4C2FD" modified="2012-04-18" version="2">MSLFDILAFKLCQCGLYETVRILKEEEIESDVLDMITENMLFKIGVKPLGVRLRVLKLISQLPREIKLEVQENVPTEVIDVPTSSMRFEEESSGHTDQLRQDMIFETSTEQGKQISEVPETHVGAMNRYISHDEASMSSSSCENDESLMININSHSLVNELPSPIPYPNFSMFVLRAEKEGSVLSVRSHIIRECAVFYLALVPNLSKHYSPICRRIIQRFPQLMDKVFVPGKHRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F6TZ66</accession>
    <name>F6TZ66_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
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    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
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                <person name="Wang H.G."/>
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                <person name="Yoshizaki F."/>
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                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
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    <reference evidence="15" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
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                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="15">wild type</strain>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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            <text>ATP + H2O = ADP + phosphate + H(+)</text>
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        <cofactor evidence="1">
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Saiga H."/>
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                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
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                <person name="Boore J."/>
                <person name="Branno M."/>
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                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Non-lysosomal glucosylceramidase that catalyzes the hydrolysis of glucosylceramide (GlcCer) to free glucose and ceramide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a beta-D-glucosyl-(1&lt;-&gt;1')-N-acylsphing-4-enine + H2O = an N-acylsphing-4-enine + D-glucose</text>
            <dbReference type="Rhea" id="RHEA:13269"/>
            <dbReference type="ChEBI" id="CHEBI:4167"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:22801"/>
            <dbReference type="ChEBI" id="CHEBI:52639"/>
            <dbReference type="EC" id="3.2.1.45"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the non-lysosomal glucosylceramidase family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.45" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="F6U9S8"/>
    <dbReference type="FunCoup" id="F6U9S8">
        <property type="interactions" value="19"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000007158"/>
    <dbReference type="Ensembl" id="ENSCINT00000007158.3">
        <property type="protein sequence ID" value="ENSCINP00000007158.3"/>
        <property type="gene ID" value="ENSCING00000003478.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000010998"/>
    <dbReference type="HOGENOM" id="CLU_006322_1_0_1"/>
    <dbReference type="InParanoid" id="F6U9S8"/>
    <dbReference type="OMA" id="HDLGAPN"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008422">
        <property type="term" value="F:beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004348">
        <property type="term" value="F:glucosylceramidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007409">
        <property type="term" value="P:axonogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007417">
        <property type="term" value="P:central nervous system development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006680">
        <property type="term" value="P:glucosylceramide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014551">
        <property type="entry name" value="B_Glucosidase_GBA2-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006775">
        <property type="entry name" value="GH116_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024462">
        <property type="entry name" value="GH116_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052566">
        <property type="entry name" value="Non-lysos_glucosylceramidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12654">
        <property type="entry name" value="BILE ACID BETA-GLUCOSIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12654:SF0">
        <property type="entry name" value="NON-LYSOSOMAL GLUCOSYLCERAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04685">
        <property type="entry name" value="DUF608"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12215">
        <property type="entry name" value="Glyco_hydr_116N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028944">
        <property type="entry name" value="Beta_gluc_GBA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyl-hydrolase family 116 N-terminal" evidence="3">
        <location>
            <begin position="14"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl-hydrolase family 116 catalytic region" evidence="2">
        <location>
            <begin position="419"/>
            <end position="781"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR028944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000007158.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="785" mass="89605" checksum="D24830EBE658397F" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>F6UFU1</accession>
    <name>F6UFU1_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">wild type</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002677">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="EAAA01002678">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6UFU1"/>
    <dbReference type="STRING" id="7719.ENSCINP00000024387"/>
    <dbReference type="Ensembl" id="ENSCINT00000024633.2">
        <property type="protein sequence ID" value="ENSCINP00000024387.2"/>
        <property type="gene ID" value="ENSCING00000013237.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164794"/>
    <dbReference type="HOGENOM" id="CLU_000022_59_2_1"/>
    <dbReference type="InParanoid" id="F6UFU1"/>
    <dbReference type="OMA" id="RDPYTCG"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016405">
        <property type="term" value="F:CoA-ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05911">
        <property type="entry name" value="Firefly_Luc_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.30:FF:000007">
        <property type="entry name" value="4-coumarate--CoA ligase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.980">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.38.10">
        <property type="entry name" value="Luciferase, Domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24096:SF149">
        <property type="entry name" value="AMP-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24096">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="4">
        <location>
            <begin position="5"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="5">
        <location>
            <begin position="404"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000024387.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="496" mass="56003" checksum="7BC9324A0BC64A81" modified="2012-04-18" version="2">VDATTEQSFTFNQVCDLSIKFASVLNKRGLRRQEVVAVCCSNCIEYPILVLGAAANNAISTTCNPYYTYLTDEMLKQFQHCQPKFVITDADQVEKVKQIADQVKYEMLKQFQHCQPKFVITNADQVEKVKQIADQAAFPTNVSFDVKEDVLLLPYSSGTTGFPKGVMHTHYSFVSLMHYAMHTKPPMRLVTYTCIPLFHILGILRQFANLIKGWKHIIDKRFNVEQLLKCVEKYKVNTMTSVPPMLVALQNYQHFDKYDTSSLKIVGSGAAPLALTVKNKTSKNLGVDIVQGWGLTEMLVSVHRSPNYPEGSVGQLMPNTQFKVVDPDSLKELGINEDGECWVKGPQLMKGYYKNQSETSRCITSDGWFRTGDIGHYDENGFIFIVDRLKELIKYKAFQVPPAELESVILSNPKVADVGVTGIPDPEAGEVPRAYVVRKDGTLTEEELNNFVQSRVSKYKYLYGGIKFVNSIPKSPTGKILRRKLHEHAFKELNKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>F6UH59</accession>
    <accession>A0A1W2WCN6</accession>
    <name>F6UH59_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Adipocyte plasma membrane-associated protein-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100182272</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">wild type</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the strictosidine synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002951">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002128935.1">
        <property type="nucleotide sequence ID" value="XM_002128899.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6UH59"/>
    <dbReference type="STRING" id="7719.ENSCINP00000024051"/>
    <dbReference type="Ensembl" id="ENSCINT00000024297.2">
        <property type="protein sequence ID" value="ENSCINP00000024051.2"/>
        <property type="gene ID" value="ENSCING00000013012.2"/>
    </dbReference>
    <dbReference type="GeneID" id="100182272"/>
    <dbReference type="KEGG" id="cin:100182272"/>
    <dbReference type="GeneTree" id="ENSGT00440000039984"/>
    <dbReference type="HOGENOM" id="CLU_637695_0_0_1"/>
    <dbReference type="InParanoid" id="F6UH59"/>
    <dbReference type="OMA" id="RWVDFAF"/>
    <dbReference type="OrthoDB" id="5307922at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018119">
        <property type="entry name" value="Strictosidine_synth_cons-reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10426:SF88">
        <property type="entry name" value="ADIPOCYTE PLASMA MEMBRANE-ASSOCIATED PROTEIN HEMOMUCIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10426">
        <property type="entry name" value="STRICTOSIDINE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03088">
        <property type="entry name" value="Str_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63829">
        <property type="entry name" value="Calcium-dependent phosphotriesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Strictosidine synthase conserved region" evidence="5">
        <location>
            <begin position="195"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="389"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="407"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000024051.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="430" mass="48188" checksum="C696D061371C855F" modified="2012-04-18" version="2">MAQIYGTKKKSRFRFKANCKKLARLLFNPRIILPPILALACLVAALSMLAVKLSSLEPVDMMTNFTNGGVWDDAFELNDILTNVRMIRTLKVFSPRCLVFDYGSQVLFVGQVDGTVLGIEKDQYGRVGMGEIKLVSSYPGYIISLRISMGMLYVGDADHGIYIIELSSMKRIMAMELHEVNPPVKSLMDFTIGTGGLLYVVDSSSKYSARTYMHQFLEGSCTGRLFRFSPVSKRVINMKDGLCMPTSVEATFSEDGLLISESGRGRFIVWDIDNTTVRSETYLPGIPGRIRLNRRGGYWMTMQAVNHGFVKYVNDRPWLRKVFCFLLPERILWGLAFTPHHAAVDLHTNGTVMASLQDQYGKNNDRITDIAEAENEVLFFGNDNQNFMGGLGDPQPQFDPKSFGMEDAPKPSDKGSSDKDPKEQETKQGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>F6UMM5</accession>
    <name>F6UMM5_CIOIN</name>
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        <recommendedName>
            <fullName evidence="4">26S proteasome non-ATPase regulatory subunit 13</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">26S proteasome regulatory subunit RPN9</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">26S proteasome regulatory subunit S11</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">26S proteasome regulatory subunit p40.5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100184104</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">wild type</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the 19S proteasome regulatory particle complex. The 26S proteasome consists of a 20S core particle (CP) and two 19S regulatory subunits (RP). The regulatory particle is made of a lid composed of 9 subunits including PSMD13, a base containing 6 ATPases and few additional components.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the proteasome subunit S11 family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001573">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6UMM5"/>
    <dbReference type="FunCoup" id="F6UMM5">
        <property type="interactions" value="1160"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000013974"/>
    <dbReference type="Ensembl" id="ENSCINT00000013974.3">
        <property type="protein sequence ID" value="ENSCINP00000013974.3"/>
        <property type="gene ID" value="ENSCING00000006810.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000001802"/>
    <dbReference type="HOGENOM" id="CLU_042989_0_0_1"/>
    <dbReference type="InParanoid" id="F6UMM5"/>
    <dbReference type="OMA" id="ANKRTIT"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008541">
        <property type="term" value="C:proteasome regulatory particle, lid subcomplex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054179">
        <property type="entry name" value="PSD13_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035298">
        <property type="entry name" value="PSMD13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10539">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10539:SF0">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22037">
        <property type="entry name" value="PSD13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0647">Proteasome</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="9">
        <location>
            <begin position="171"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006207"/>
        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000013974.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="376" mass="43073" checksum="D4CE906674A9692E" modified="2012-04-18" version="2">EDFLKEFNTSGLPAEALEEFKQIEELYTKKLWHQLTESLVRFVKYDCFKQGTMLLDIYKHFISDFESKINPLALMEMLCPFNLFYVFTDSAEALAFLEKIKEKSKANLEAKLLCLTAIGNIHLLGNNFEATKKVMEECEAILGDLTGISSVHGRYYELCSNYHQILSHHNEYYKDALRYLGCTDLDTISVPEQQERAFNLGLAGILGDKVFNFGELLQHPVLDSLKDTPRQWLIDLLLTFNSGDIEQLNQLRPYWSAQPDLIANELKLKQKIMLLCLMEMTFARPSNNRHLKFTEIATNTGIPVEEVEILAMKAMSLGLVQGTIDQVEEEIHMQWVQPRVLDKQQVGKMKGKLSTWCNDVLSMESLVSSNAQEILT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
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    <name>F6URK3_CIOIN</name>
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        <recommendedName>
            <fullName evidence="17">SH3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
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            <taxon>Ciona</taxon>
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    <reference evidence="16" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">wild type</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Filopodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Lamellipodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ABI family.</text>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="FunCoup" id="F6URK3">
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    <dbReference type="Ensembl" id="ENSCINT00000023766.2">
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        <property type="gene ID" value="ENSCING00000012655.2"/>
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    <dbReference type="InParanoid" id="F6URK3"/>
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    <dbReference type="Proteomes" id="UP000008144">
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="GO_Central"/>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="evidence" value="ECO:0000318"/>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="project" value="GO_Central"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="ABI"/>
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        <property type="entry name" value="ABI3_SH3"/>
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        <property type="entry name" value="SH3-like_dom_sf"/>
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        <property type="entry name" value="T_SNARE_dom"/>
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        <property type="entry name" value="ABELSON INTERACTING PROTEIN, ISOFORM D"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00499">
        <property type="entry name" value="P67PHOX"/>
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        <property type="entry name" value="SH3DOMAIN"/>
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        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="46"/>
            <end position="108"/>
        </location>
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        <location>
            <begin position="383"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="151"/>
            <end position="178"/>
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        <location>
            <begin position="232"/>
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    <feature type="compositionally biased region" description="Pro residues" evidence="12">
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            <begin position="238"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="257"/>
            <end position="287"/>
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        <location>
            <begin position="361"/>
            <end position="375"/>
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    </evidence>
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    </evidence>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="441" mass="47462" checksum="AA8A766638A52290" modified="2012-04-18" version="2">NMAELQMLLEQEIPAGRNALLDSHNNLKKVATYCQQRYIEDPNKKTALEETKNYTTQSLASVAYQINTLASNMLQMLDIQAAQLNSMDSAINNISQTVDIHKEKVARREIGLLTTNKIITRSHQIVAPTTQDRPVKYVRKPVDYTELDDIGHGVKLNSQPNAGTMRRPSAVSTKKTQSPSEGLKSILYLLFHSGSKRDSLKSNVSGYGTLRPVKAPTVPQEYSQITMVTKLGSTPVSSMPPPPPQAPTPPASNNISAPPPPPPPAMTSSIPPPPPPMNSSMPPPPPFGAMSPQGVPPPPPVLLPSATTSPESSGGYSTPNTVMDLPPPPPDDFMSSRPPYIGGVPPMAGGDVLPPPPPSMGTPSSYDSSPGDYSTEPSWAPKSYLEKVVVIYDYSAENSDELNLKEDQVVYVVRKNDDGWYEGVLDGVTGLFPGNYAEAVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F6UX23</accession>
    <name>F6UX23_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
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                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
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                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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        <property type="entry name" value="Ser/Thr_kinase_AS"/>
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    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
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        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
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        <property type="entry name" value="Pkinase"/>
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    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="24"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
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        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000024026.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="214" mass="23504" checksum="7222212B04EAACAD" modified="2012-04-18" version="2">MSNHGDTDILSKYFYKAHEFTTSGYPGDFIGSGAYGVVIRCDHRKHGVVAVKCMITAGKDFGEMQIEKRMTDEAKMILKSSHDNTNTNIVKLLGITKWEGSVGLVMEYMAYGSLETVVFKYTVPELFWDVRMFIVNQLVNGINYMHCLKKPIVHGDLKPENVLLTNGLVTKIGDFGASAMASCTGSTVQGSATSGNARGGNYQHTIAYTPPEML</sequence>
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    <accession>F6VAC9</accession>
    <accession>A0A1W2WG37</accession>
    <name>F6VAC9_CIOIN</name>
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        <submittedName>
            <fullName evidence="2">Valacyclovir hydrolase-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC100176213</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01002843">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002127049.2">
        <property type="nucleotide sequence ID" value="XM_002127013.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6VAC9"/>
    <dbReference type="FunCoup" id="F6VAC9">
        <property type="interactions" value="17"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000010598"/>
    <dbReference type="ESTHER" id="cioin-f6vac9">
        <property type="family name" value="Valacyclovir-hydrolase"/>
    </dbReference>
    <dbReference type="MEROPS" id="S33.A92"/>
    <dbReference type="Ensembl" id="ENSCINT00000010598.3">
        <property type="protein sequence ID" value="ENSCINP00000010598.3"/>
        <property type="gene ID" value="ENSCING00000005148.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100176213"/>
    <dbReference type="KEGG" id="cin:100176213"/>
    <dbReference type="GeneTree" id="ENSGT00390000004746"/>
    <dbReference type="HOGENOM" id="CLU_020336_50_5_1"/>
    <dbReference type="InParanoid" id="F6VAC9"/>
    <dbReference type="OMA" id="RFPQLWA"/>
    <dbReference type="OrthoDB" id="19657at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017171">
        <property type="term" value="F:serine hydrolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46331">
        <property type="entry name" value="VALACYCLOVIR HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46331:SF2">
        <property type="entry name" value="VALACYCLOVIR HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="55"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000010598.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="292" mass="33757" checksum="8E12551BC0BD2129" modified="2011-07-27" version="1">MLFKQFLQSTAKTQCWSFLNRGLYINKKSTRTMGSKVMVNGVNLYYEKVGTGEHVVLLVPGALGSTKTDFKPQLENLNRDKFTVIGWDPRGYGQSRPPNRECKPDFIFRDAEDAVSLMENLGFETYSLLGWSDGANISAVMAARWSERVRKLVIWGGNSYFTQQELELYQSIRDLSTWSKSMLNPMVEVYGKEYFEKTWHAWIDMFTEMAGGRKEDTNLYNEELCKIKADTLIIHGQKDALVPDFHPKHFAKNIQNSRLVVWEDGKHNLHLRFADRFNELVQSFLLENTSNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>F6VS24</accession>
    <name>F6VS24_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sema domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
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            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">wild type</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
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                <person name="De Tomaso A."/>
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                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
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                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
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                <person name="Makabe K.W."/>
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                <person name="Mochizuki Y."/>
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                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
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                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
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                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
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                <person name="Doggett N."/>
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                <person name="Richardson P."/>
                <person name="Lucas S."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
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                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">wild type</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulin"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014090298" evidence="3">
        <location>
            <begin position="20"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="245"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="39"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="142"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="281"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="282"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000012003.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="327" mass="35257" checksum="7C79C3716F392232" modified="2012-04-18" version="2">MNFNVLLCVVLAFVCCCNGVRVTLLNGAASPLPIEVTLGNPITLACSYQPENGNAQDARLEWLYSTNMDGTSTSGQNIVLDGIIQPNFNNGRFSIVNPSSLQISESIKTDTGSYTCLVTLPNDTPSKGSGTYHLTVNVPPSPPQCQYTSDHALTIGFAAEFRCLSVEGIPTPTYTWFKNGRQLPLSAQDDVTYKNASFSYSATTGVLKFSKVTTGDAGTYYCSSSNKIATESCLPTVITTKNQDVGMIVGIVFGVLFGLILIGVLVWWTWRKGYCDGLSKEDDPDEIEDDGSNDIMLDTNGPTVHKPPSEVSSTRKPYPDGAVRVMP</sequence>
</entry>
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    <accession>F6WHP2</accession>
    <accession>A0A1W2W9Q2</accession>
    <name>F6WHP2_CIOIN</name>
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        <submittedName>
            <fullName evidence="3">Zinc finger MYND domain-containing protein 12</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100176509</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01000627">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002127183.1">
        <property type="nucleotide sequence ID" value="XM_002127147.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6WHP2"/>
    <dbReference type="Ensembl" id="ENSCINT00000015987.3">
        <property type="protein sequence ID" value="ENSCINP00000015987.3"/>
        <property type="gene ID" value="ENSCING00000007810.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100176509"/>
    <dbReference type="KEGG" id="cin:100176509"/>
    <dbReference type="GeneTree" id="ENSGT00390000004248"/>
    <dbReference type="HOGENOM" id="CLU_058444_1_1_1"/>
    <dbReference type="InParanoid" id="F6WHP2"/>
    <dbReference type="OMA" id="KILFMLY"/>
    <dbReference type="OrthoDB" id="3174329at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053248">
        <property type="entry name" value="Zinc_finger_MYND_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46533">
        <property type="entry name" value="ZINC FINGER MYND DOMAIN-CONTAINING PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46533:SF1">
        <property type="entry name" value="ZINC FINGER MYND DOMAIN-CONTAINING PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="202"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000015987.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="397" mass="44660" checksum="51ABAD45D6B17145" modified="2011-07-27" version="1">MSSWQHVKENESTQAQEELERSRQKIQTALKAAKERSNKNSNSNDDIDTKFNQLQNLGIKLLDILRDSGTDQHPDWINLTERMCQLLSPLEGSMPFMSSSEEREIRKKDLLTKQTKMMELTRIIGRKLLFEGKHNQAVPAAVQSLRFSIDVHGLNSVNLVPSYLILAEASIGLGKLSQGDEYLAQAEWTVLKSPDATAAVKSKLYRNIGLLYAAKGDYDNALRMLSEDIYHASKEWGTTDIHACGGYFHMANVFFRQNKMDVADSLYSQVTDNWFDFLKESVQRKIASPAATGLLGPTEKVELDDVVMLDEAQEAEAMQMLNAVLDIREQSPKQNNLSIAKVCHTLSMLYFLLNDIQKAQELSSKAQDASSRLPTNVETVSVPTFLKLSHEKSTVRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>F6WNH9</accession>
    <name>F6WNH9_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="F6WNH9"/>
    <dbReference type="FunCoup" id="F6WNH9">
        <property type="interactions" value="419"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000006737"/>
    <dbReference type="Ensembl" id="ENSCINT00000006737.3">
        <property type="protein sequence ID" value="ENSCINP00000006737.3"/>
        <property type="gene ID" value="ENSCING00000003276.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01120000271863"/>
    <dbReference type="HOGENOM" id="CLU_038321_3_1_1"/>
    <dbReference type="InParanoid" id="F6WNH9"/>
    <dbReference type="OMA" id="FRVYSHM"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030295">
        <property type="term" value="F:protein kinase activator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035329">
        <property type="term" value="P:hippo signaling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.30:FF:000001">
        <property type="entry name" value="MOB kinase activator 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.30">
        <property type="entry name" value="MOB kinase activator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005301">
        <property type="entry name" value="MOB_kinase_act_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036703">
        <property type="entry name" value="MOB_kinase_act_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22599">
        <property type="entry name" value="MPS ONE BINDER KINASE ACTIVATOR-LIKE MOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03637">
        <property type="entry name" value="Mob1_phocein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01388">
        <property type="entry name" value="Mob1_phocein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101152">
        <property type="entry name" value="Mob1/phocein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="1">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR605301-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000006737.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="219" mass="25410" checksum="3A5DD57D83A2AC91" modified="2012-04-18" version="2">MSFFFQNRHNKTFKPHKSIPEGSHQHELIRHAAATLGSGNLQLAVALPEGEDLNEWIAVNTVDFFNQINMLYGTISEFCTATKCEVMSAGPKYEYHWADGVTIKKPIKCSAPRYIDYLMTWVQCQLDNEEIFPSAVGVPFSKNFMSIAKTILKRLFRVYAHIYHQHFSDVMGLGEEAHLNTSFKHFIYFVQEFNLIDRRELAPLQELIDKLLSRDREKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>F6WYJ4</accession>
    <name>F6WYJ4_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tr-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">wild type</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01002121">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6WYJ4"/>
    <dbReference type="STRING" id="7719.ENSCINP00000017752"/>
    <dbReference type="Ensembl" id="ENSCINT00000017752.3">
        <property type="protein sequence ID" value="ENSCINP00000017752.3"/>
        <property type="gene ID" value="ENSCING00000008660.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154662"/>
    <dbReference type="HOGENOM" id="CLU_002794_8_1_1"/>
    <dbReference type="InParanoid" id="F6WYJ4"/>
    <dbReference type="OMA" id="HGNGFLI"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908:SF10">
        <property type="entry name" value="EUKARYOTIC TRANSLATION ELONGATION FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908">
        <property type="entry name" value="TRANSLATION ELONGATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="4">
        <location>
            <begin position="5"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000017752.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="117" mass="12769" checksum="2791CD17858F5FBE" modified="2012-04-18" version="2">MDKKSNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGSKAGEARFTDTRKDEQDRCITIKSTAISMYYELSDRDMQWVEGQKHGNGFLINLIDSPGHVDFSSEVTAALRVTDGALVVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>F6X229</accession>
    <name>F6X229_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Calcium-transporting ATPase</fullName>
            <ecNumber evidence="19">7.2.2.10</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="19">Catalyzes the hydrolysis of ATP coupled with the transport of calcium.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>Ca(2+)(in) + ATP + H2O = Ca(2+)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18105"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.2.2.10"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:18106"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="19">Membrane</location>
            <topology evidence="19">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Sarcoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.10" evidence="19"/>
    <dbReference type="AlphaFoldDB" id="F6X229"/>
    <dbReference type="FunCoup" id="F6X229">
        <property type="interactions" value="56"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000017173"/>
    <dbReference type="Ensembl" id="ENSCINT00000017173.3">
        <property type="protein sequence ID" value="ENSCINP00000017173.3"/>
        <property type="gene ID" value="ENSCING00000008397.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155668"/>
    <dbReference type="HOGENOM" id="CLU_002360_3_2_1"/>
    <dbReference type="InParanoid" id="F6X229"/>
    <dbReference type="OMA" id="HGPMISE"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033017">
        <property type="term" value="C:sarcoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005388">
        <property type="term" value="F:P-type calcium transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070588">
        <property type="term" value="P:calcium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02083">
        <property type="entry name" value="P-type_ATPase_SERCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000003">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000005">
        <property type="entry name" value="Calcium-transporting ATPase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000065">
        <property type="entry name" value="Sarcoplasmic/endoplasmic reticulum calcium ATPase 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000160">
        <property type="entry name" value="Sarcoplasmic/endoplasmic reticulum calcium ATPase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005782">
        <property type="entry name" value="P-type_ATPase_IIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01116">
        <property type="entry name" value="ATPase-IIA1_Ca"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42861">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08282">
        <property type="entry name" value="Hydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 19" id="KW-0106">Calcium</keyword>
    <keyword evidence="7 19" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="16 19" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="17 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0703">Sarcoplasmic reticulum</keyword>
    <keyword evidence="14" id="KW-1278">Translocase</keyword>
    <keyword evidence="8 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5 19" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="107"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="139"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="604"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="637"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="677"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="772"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase A" evidence="20">
        <location>
            <begin position="3"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase C-terminal" evidence="21">
        <location>
            <begin position="628"/>
            <end position="831"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU361146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF00689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000017173.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="847" mass="93120" checksum="AEAB3DE13361DE2D" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>F6X245</accession>
    <name>F6X245_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sulfotransferase</fullName>
            <ecNumber evidence="3">2.8.2.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">wild type</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the sulfotransferase 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="3"/>
    <dbReference type="EMBL" id="EAAA01000963">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6X245"/>
    <dbReference type="Ensembl" id="ENSCINT00000029395.2">
        <property type="protein sequence ID" value="ENSCINP00000029149.2"/>
        <property type="gene ID" value="ENSCING00000017064.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163815"/>
    <dbReference type="HOGENOM" id="CLU_027239_1_2_1"/>
    <dbReference type="InParanoid" id="F6X245"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051923">
        <property type="term" value="P:sulfation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11783">
        <property type="entry name" value="SULFOTRANSFERASE SULT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Sulfotransferase" evidence="4">
        <location>
            <begin position="16"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000029149.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="297" mass="34907" checksum="B651890F0534C1D3" modified="2012-04-18" version="2">TQEIFDSALAYEPDSSDIFVVTYPKCGTTWMLNILYLIEHGGKMIGSDDNLNFHYPFIELVGGDKVQEFKPPRVIKTHLDDRMIKHKEGVKCIFVARNPKDCVVSFFHATHDFHNLYKYEGGKFDDYFKIFMQGRVDWGDYFVMVPRWHEKSLREKDVHFMLYEDMKNDPKMEIEKLADFLGPKWSDSIKREGVLENVIKNSSFKAMKSNPLKYVSSINKVDNMQLTISLRLQCITIAPKDLKFMRKGVVGDWRNTLSEEQSREIDEKMKTVGKDYPGFDKIWEKYPDLFEKRSQSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>F6XNI1</accession>
    <name>F6XNI1_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FERM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01001948">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6XNI1"/>
    <dbReference type="STRING" id="7719.ENSCINP00000024879"/>
    <dbReference type="Ensembl" id="ENSCINT00000025125.2">
        <property type="protein sequence ID" value="ENSCINP00000024879.2"/>
        <property type="gene ID" value="ENSCING00000019730.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162787"/>
    <dbReference type="HOGENOM" id="CLU_1177695_0_0_1"/>
    <dbReference type="InParanoid" id="F6XNI1"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000299">
        <property type="entry name" value="FERM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018979">
        <property type="entry name" value="FERM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047145">
        <property type="entry name" value="FRMD6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13429">
        <property type="entry name" value="FERM DOMAIN (PROTEIN4.1-EZRIN-RADIXIN-MOESIN) FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13429:SF5">
        <property type="entry name" value="PROTEIN EXPANDED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09379">
        <property type="entry name" value="FERM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50057">
        <property type="entry name" value="FERM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FERM" evidence="1">
        <location>
            <begin position="155"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000024879.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="236" mass="26466" checksum="AC6EE9F15B09EAC8" modified="2012-04-18" version="2">CRANVLSAIPYKKKQQAVTSREHSIVAGKWNHFSVGDNQGDNSPPYQRRRASLFTVVATAPACGQNLQRDRSNQPDKELGNCKPADCKAVTVADTDRLKPIVFESRTRNSTVVPVIYQEAASGKRAVFDKEQQKLQCGRRERALAAIMKSNSGKKPVRVHLLTGDSILLVFDAKALVKDVFDQVCTMYSIKESHFFGLSAVIENEHRFMDTKQRLSKYAPKEWGKDLKRRASDLSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>F6XS17</accession>
    <accession>A0A1W5BE93</accession>
    <name>F6XS17_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glutaminyl-peptide cyclotransferase</fullName>
            <ecNumber evidence="3">2.3.2.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100175874</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>N-terminal L-glutaminyl-[peptide] = N-terminal 5-oxo-L-prolyl-[peptide] + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:23652"/>
            <dbReference type="Rhea" id="RHEA-COMP:11736"/>
            <dbReference type="Rhea" id="RHEA-COMP:11846"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:64722"/>
            <dbReference type="ChEBI" id="CHEBI:87215"/>
            <dbReference type="EC" id="2.3.2.5"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glutaminyl-peptide cyclotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.5" evidence="3"/>
    <dbReference type="RefSeq" id="XP_018670675.1">
        <property type="nucleotide sequence ID" value="XM_018815130.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6XS17"/>
    <dbReference type="FunCoup" id="F6XS17">
        <property type="interactions" value="12"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000005157"/>
    <dbReference type="Ensembl" id="ENSCINT00000005156.3">
        <property type="protein sequence ID" value="ENSCINP00000005157.3"/>
        <property type="gene ID" value="ENSCING00000002529.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100175874"/>
    <dbReference type="GeneTree" id="ENSGT00390000003107"/>
    <dbReference type="HOGENOM" id="CLU_045003_1_2_1"/>
    <dbReference type="InParanoid" id="F6XS17"/>
    <dbReference type="OMA" id="HYETNYP"/>
    <dbReference type="OrthoDB" id="3907302at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016603">
        <property type="term" value="F:glutaminyl-peptide cyclotransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000196">
        <property type="entry name" value="glutaminyl-peptide cyclotransferase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007484">
        <property type="entry name" value="Peptidase_M28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040234">
        <property type="entry name" value="QC/QCL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12283">
        <property type="entry name" value="GLUTAMINYL-PEPTIDE CYCLOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12283:SF6">
        <property type="entry name" value="GLUTAMINYL-PEPTIDE CYCLOTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04389">
        <property type="entry name" value="Peptidase_M28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M28" evidence="7">
        <location>
            <begin position="164"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000005157.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="401" mass="46432" checksum="59A8323ED4DFA88A" modified="2012-04-18" version="2">MLSIESINSIMSRYRRPKESSVSETPQHPLSNRHTNYKRTLFIGGIGITLIAVVVLFNHLQSTEVKGPVEIERNYETWHLAKNSWQPRVLNREELTQIAQKVDQVDIKPIFNSFMIPRPPGTLNITIVGMYIRHKMEQELGWTVEEMTFTDQPPHPYPPTQFKNIIATHNPGAARRLVLACHYDSKITPTGFLGACDSAMPCSMMIEIAFALREYLDQRKLQQQQGIDRSDLTLEFLFFDGEEAFDTWTETDSLYGSRHMASRWTSPWNGRSRLDSIDLFVLLDLIGTTDTQFYNLPVTSSRWFNHLAELELQMYRAGLLTVNNTRHTHRIFIPRTANYNVEDDHVPFHRAGVPIMHLISVPFPTVWHEITDNEFAMNYERTEVISRVMTAFVAKYLQLNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F6XV42</accession>
    <name>F6XV42_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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                <person name="De Tomaso A."/>
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                <person name="Meinertzhagen I.A."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <property type="entry name" value="WD40_repeat_dom_sf"/>
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        <property type="entry name" value="WD40_rpt"/>
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        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 47"/>
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        <location>
            <begin position="57"/>
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    <feature type="repeat" description="WD" evidence="3">
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            <begin position="149"/>
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    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="238"/>
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    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="285"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    </evidence>
    <sequence length="320" mass="35073" checksum="DACCBF235622B317" modified="2012-04-18" version="2">EDPSAIRAVAFHPRGEVFAVGSNSQILKICSMPRQRYQDSHLELLGTGVLDVCVKLPKVHKGSIYSTAWNDDGSMLATCSNDKLIKLFDYSSTTMNVTGPSLELTHHNAPVRDVAFLTNYGSACVLANAGADCIVYTNDCEVDAMLHKLSGHSEQVLSLYAWGSGMLVSTSQDKSVRTWDLRTSRCVSVLGFSDASDQSPSVAASVCVDTRGRYMAVGRTDNMISIYDFNAGKIIKTYQAHNNEVRSVRFNPVCPSVLLSGSYDGTAYVTDIEALFLSGLHLLNIPYHKDKVIQCRWHPSGRFFATTSADQTCVLWTAYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>F6YDE8</accession>
    <accession>A0A1W3JPA1</accession>
    <name>F6YDE8_CIOIN</name>
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        <recommendedName>
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        <alternativeName>
            <fullName evidence="13">Polyglutamine tract-binding protein 1</fullName>
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    </protein>
    <gene>
        <name evidence="17" type="primary">LOC104265809</name>
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    <organism evidence="17 18">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">wild type</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="14">Interacts with POU3F2/Brn-2, ATXN1, TXNL4A, HTT and AR. Interaction with ATXN1 correlates positively with the length of the polyglutamine tract. Interacts with RNA polymerase II large subunit in a phosphorylation-dependent manner. Forms a ternary complex with ATXN1 mutant and phosphorylated RNA polymerase II. Interacts (via C-terminus) with TXNL4A and CD2BP2. Interacts (via WW domain) with ATN1 and SF3B1, and may interact with additional splice factors. Interacts (via WW domain) with WBP11; Leading to reduce interaction between PQBP1 and TXNL4A. Interacts with CAPRIN1. Interacts with DDX1. Interacts with SFPQ. Interacts with KHSRP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic granule</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus speckle</location>
        </subcellularLocation>
    </comment>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="RefSeq" id="XP_009858953.1">
        <property type="nucleotide sequence ID" value="XM_009860651.3"/>
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    <dbReference type="AlphaFoldDB" id="F6YDE8"/>
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        <property type="interactions" value="356"/>
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    <dbReference type="STRING" id="7719.ENSCINP00000005423"/>
    <dbReference type="Ensembl" id="ENSCINT00000005423.3">
        <property type="protein sequence ID" value="ENSCINP00000005423.3"/>
        <property type="gene ID" value="ENSCING00000002659.3"/>
    </dbReference>
    <dbReference type="GeneID" id="104265809"/>
    <dbReference type="KEGG" id="cin:104265809"/>
    <dbReference type="GeneTree" id="ENSGT00950000183102"/>
    <dbReference type="HOGENOM" id="CLU_043596_1_0_1"/>
    <dbReference type="InParanoid" id="F6YDE8"/>
    <dbReference type="OMA" id="IYHECSK"/>
    <dbReference type="OrthoDB" id="42462at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0016604">
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        <property type="evidence" value="ECO:0000318"/>
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        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:ribonucleoprotein complex binding"/>
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        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="POLYGLUTAMINE BINDING PROTEIN 1/MARVEL MEMBRANE-ASSOCIATING DOMAIN CONTAINING 3"/>
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        <property type="entry name" value="POLYGLUTAMINE-BINDING PROTEIN 1"/>
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    <keyword evidence="11" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
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    <feature type="compositionally biased region" description="Low complexity" evidence="15">
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            <begin position="92"/>
            <end position="103"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
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    <feature type="compositionally biased region" description="Basic residues" evidence="15">
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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        <source>
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    <evidence type="ECO:0000256" key="14">
        <source>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50020"/>
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        <source>
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        <source>
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        </source>
    </evidence>
    <sequence length="208" mass="23317" checksum="7BA215098B644B34" modified="2011-07-27" version="1">MPLPAALAARLAKRGIVSSETQDSSNPDEEVIAEDYDIPGQEAHVDEFGKHLPPMWEKVWEPSHETWYYWDTVNDKVSWLPPTDPDAKITNPAPKKSPFAPAPLGERANHFSEPVDYRKEEKKSKHKSAAKRKAPRPPEEFDPMDPSSYSDAPRGKWSVGLKKVDDAKSGVDSTANGPLFQQRPYPSPGEVLSRNKQARREVGPLKPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>F6YG13</accession>
    <name>F6YG13_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="21">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">wild type</strain>
        </source>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="17">Postsynaptic cell membrane</location>
            <topology evidence="17">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the ligand-gated ion channel (TC 1.A.9) family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002898">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6YG13"/>
    <dbReference type="FunCoup" id="F6YG13">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000008772"/>
    <dbReference type="Ensembl" id="ENSCINT00000008772.3">
        <property type="protein sequence ID" value="ENSCINP00000008772.3"/>
        <property type="gene ID" value="ENSCING00000004249.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156722"/>
    <dbReference type="HOGENOM" id="CLU_010920_2_2_1"/>
    <dbReference type="InParanoid" id="F6YG13"/>
    <dbReference type="OMA" id="CFLWFPS"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902711">
        <property type="term" value="C:GABA-A receptor complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005230">
        <property type="term" value="F:extracellular ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004890">
        <property type="term" value="F:GABA-A receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902476">
        <property type="term" value="P:chloride transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007214">
        <property type="term" value="P:gamma-aminobutyric acid signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904862">
        <property type="term" value="P:inhibitory synapse assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000003">
        <property type="entry name" value="Putative gamma-aminobutyric acid receptor subunit gamma-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006028">
        <property type="entry name" value="GABAA/Glycine_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001390">
        <property type="entry name" value="GABAAa_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00860">
        <property type="entry name" value="LIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01079">
        <property type="entry name" value="GABAARALPHA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00253">
        <property type="entry name" value="GABAARECEPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0868">Chloride</keyword>
    <keyword evidence="11" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16 18" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 18" id="KW-0406">Ion transport</keyword>
    <keyword evidence="15" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="8 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0770">Synapse</keyword>
    <keyword evidence="3 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 18" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="214"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="250"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="280"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="427"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="19">
        <location>
            <begin position="2"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="20">
        <location>
            <begin position="221"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU000687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000008772.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="446" mass="51488" checksum="85FEFEF470A5BBE5" modified="2012-04-18" version="2">ITRTLDNFLLNYDNRIRPGLEEGPTKVYSYFFVTGFGPASEQDMDYTLDMFFRQRWRDERLAFKHMNKNITELKLNNNMVQRLWTPDSFFRNGKKSIAHNITVPNRLLRIDPNGNILYTMRLTIKARCPMILIDFPMDVHTCHLVYGSYGYTKDQMQFLWYTENGRKPAVDVPSSSSRLNQFKLINYTWTTNIINTISGEYSVLETKFHLKRQMGYFVIQTYLPCILIVILSQISFWINKEAVPARTVSGIMTVLSLTMLSISTRQSLPKVAYATALDWYMAVCFAFCFAALIEFASGKLFFLSKMNYFQMQIEQSKSKEKTDSSNDKKDVIESRKGQILFLPLGVASASKNHINSSDQELARKLLLAAEAEINEAAMSQVNYGWVGCILYCSIPLKNSLIRYSWFCLKCARKDGGGVSRIDRVSRVAFPLAFFFFNGIYWFVYLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>F6YGT6</accession>
    <name>F6YGT6_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">wild type</strain>
        </source>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Functions as a component of the nuclear pore complex (NPC).</text>
    </comment>
    <comment type="function">
        <text evidence="16">Plays a role in the nuclear pore complex (NPC) assembly and/or maintenance. Required for the assembly of peripheral proteins into the NPC. May anchor NUP62 to the NPC. Involved in nephrogenesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="17">Part of the nuclear pore complex (NPC). Forms part of the Nup160 subcomplex in the nuclear pore which is composed of NUP160, NUP133, NUP107 and Nup96; this complex plays a role in RNA export and in tethering Nup98 and NUP153 to the nucleus. Does not interact with TPR. Interacts with ZNF106.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
            <location evidence="1">Centromere</location>
            <location evidence="1">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="18">Nucleus</location>
            <location evidence="18">Nuclear pore complex</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="18">Nucleus membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 18">Belongs to the nucleoporin Nup84/Nup107 family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002669">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6ZA03"/>
    <dbReference type="FunCoup" id="F6ZA03">
        <property type="interactions" value="430"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000011112"/>
    <dbReference type="Ensembl" id="ENSCINT00000011112.3">
        <property type="protein sequence ID" value="ENSCINP00000011112.3"/>
        <property type="gene ID" value="ENSCING00000005394.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000012080"/>
    <dbReference type="HOGENOM" id="CLU_012944_1_0_1"/>
    <dbReference type="InParanoid" id="F6ZA03"/>
    <dbReference type="OMA" id="MAHIVLF"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031080">
        <property type="term" value="C:nuclear pore outer ring"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000973">
        <property type="term" value="P:post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3450.20:FF:000001">
        <property type="entry name" value="Nuclear pore complex protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.190.50:FF:000001">
        <property type="entry name" value="Nuclear pore complex protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3450.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.190.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007252">
        <property type="entry name" value="Nup84/Nup107"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13003:SF2">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13003">
        <property type="entry name" value="NUP107-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04121">
        <property type="entry name" value="Nup84_Nup100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="15" id="KW-0137">Centromere</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="13 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="7" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="12 18" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="14 18" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 18" id="KW-0811">Translocation</keyword>
    <keyword evidence="3 18" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00056880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00063956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU365072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000011112.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="814" mass="93675" checksum="593CF99E13978D41" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>F6ZHF5</accession>
    <name>F6ZHF5_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein Wnt</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">wild type</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Ligand for members of the frizzled family of seven transmembrane receptors.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Secreted</location>
            <location evidence="1 10">Extracellular space</location>
            <location evidence="1 10">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the Wnt family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002747">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6ZHF5"/>
    <dbReference type="STRING" id="7719.ENSCINP00000024208"/>
    <dbReference type="Ensembl" id="ENSCINT00000024454.2">
        <property type="protein sequence ID" value="ENSCINP00000024208.2"/>
        <property type="gene ID" value="ENSCING00000006936.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000167538"/>
    <dbReference type="HOGENOM" id="CLU_033039_1_2_1"/>
    <dbReference type="InParanoid" id="F6ZHF5"/>
    <dbReference type="OMA" id="RADGWKW"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005109">
        <property type="term" value="F:frizzled binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060070">
        <property type="term" value="P:canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045165">
        <property type="term" value="P:cell fate commitment"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046330">
        <property type="term" value="P:positive regulation of JNK cascade"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd19339">
        <property type="entry name" value="Wnt_Wnt7"/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
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                <person name="Branno M."/>
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                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
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                <person name="Kobayashi K."/>
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                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
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                <person name="Mount S."/>
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                <person name="Shin-i T."/>
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                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
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                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
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                <person name="Richardson P."/>
                <person name="Lucas S."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
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                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
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                <person name="Huang W."/>
                <person name="Kawashima T."/>
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                <person name="Meinertzhagen I.A."/>
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                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
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                <person name="Kobayashi M."/>
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                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
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                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
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            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">wild type</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="entry name" value="MFS_sugar_transport-like"/>
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        <property type="entry name" value="MFS_trans_sf"/>
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    <dbReference type="PANTHER" id="PTHR24064">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 22 MEMBER"/>
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        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
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            <end position="25"/>
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    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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    <feature type="transmembrane region" description="Helical" evidence="5">
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    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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    <feature type="transmembrane region" description="Helical" evidence="5">
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    <feature type="transmembrane region" description="Helical" evidence="5">
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    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000007882.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="372" mass="41242" checksum="9C8AAD0F621F458E" modified="2012-04-18" version="2">MFAFFRTANGFLGGGVTLVLFVYLQEIVGRKWWAVTGSLLNGLFALGIVVMVGIAYAVPPWRTMIQILVIPQVVPLISLWFLPESPRWLYSQGKLAEAESVLIKIAKRNGVNHPVLSLVKKPSEHKKSSFTVIDLFKNRTTLKRLLIMGYIWFVCSFVYYGLTLAAGDIGSNPYMNELLSGVVELPTMMFTAYILDRKWCGRKRGLVVALLTAGVCCFILMFYKEKNTLKMGVGLSGKMLIATAFNIIYIYSPELFPTVVRNAALGSQSMCARVGGILSAQMKTLVAINPLVAYLLFAITGITSGLLSFFLPETLGIKPPDSFADLDSQIKKNNSARITPNVPRMVMSNLRKKENNLEQAELLLASDEDDIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>F6ZWF0</accession>
    <name>F6ZWF0_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">BICD family-like cargo adapter 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100176252</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="F6ZWF0"/>
    <dbReference type="STRING" id="7719.ENSCINP00000026405"/>
    <dbReference type="Ensembl" id="ENSCINT00000026651.2">
        <property type="protein sequence ID" value="ENSCINP00000026405.2"/>
        <property type="gene ID" value="ENSCING00000014671.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000171206"/>
    <dbReference type="HOGENOM" id="CLU_1444057_0_0_1"/>
    <dbReference type="InParanoid" id="F6ZWF0"/>
    <dbReference type="OMA" id="YTRLEHK"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051149">
        <property type="entry name" value="Spindly/BICDR_Dynein_Adapter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32123:SF13">
        <property type="entry name" value="BICAUDAL D-RELATED PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32123">
        <property type="entry name" value="BICD FAMILY-LIKE CARGO ADAPTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="68"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000026405.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="188" mass="22209" checksum="51A81E34F7F3C874" modified="2011-07-27" version="1">MDDVLYDVDVCEDESEVGSMPECQMDLTEVDVYTRLEHKERDLIIAAELGKSLLEKNEFLLQENERISVDYRDRCEILEQQKHELRQRFEFKTSEWEARVAQLSSDLRRTQEQLEDHKAMWGAAEREKSRAVDEMTQQNQKLLSQLQKSSEAERQLSIQVQQLRREFIQRSSTASVKIVELESLKEEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>F7A141</accession>
    <name>F7A141_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="F7A141"/>
    <dbReference type="STRING" id="7719.ENSCINP00000029071"/>
    <dbReference type="Ensembl" id="ENSCINT00000029317.2">
        <property type="protein sequence ID" value="ENSCINP00000029071.2"/>
        <property type="gene ID" value="ENSCING00000016990.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166564"/>
    <dbReference type="HOGENOM" id="CLU_000288_7_40_1"/>
    <dbReference type="InParanoid" id="F7A141"/>
    <dbReference type="OMA" id="CRIELFR"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004715">
        <property type="term" value="F:non-membrane spanning protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004714">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00192">
        <property type="entry name" value="PTKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000554">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050122">
        <property type="entry name" value="RTK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416:SF620">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE RECEPTOR TORSO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000615">
        <property type="entry name" value="TyrPK_CSF1-R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 9 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 9 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="9"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="134"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00051243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000615-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000615-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000615-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000029071.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="275" mass="31232" checksum="7109E68AA3C6E419" modified="2012-04-18" version="2">KWEINRDNLVVGEILGRGNYGIVYKGTYEGETPTDVAIKTMKDGLNMEQNEIEFLTEIGFMLNVGDHPNVLKVIGCCTTVKPIMLVTEYLKYGDLLHFLWDAREHVTEKSLFLMSADVATGMDFLTRSRIIHGDLAARNILVGEDLRCKISDFGLANDVYRYGAIKGQSERCVPFKWISPERMMSGKVPITSKSDVWSFGNLMYEMVTLGCMPFPDVESDGLLEKLKSGYRMKQPSSCSDEMFSIMTKCWKWKASSRPSFTNLIKLIDEETKKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>F7AFN1</accession>
    <name>F7AFN1_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">SUEL-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">wild type</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sulfotransferase 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001304">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F7AFN1"/>
    <dbReference type="Ensembl" id="ENSCINT00000026283.1">
        <property type="protein sequence ID" value="ENSCINP00000026037.1"/>
        <property type="gene ID" value="ENSCING00000014383.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164429"/>
    <dbReference type="HOGENOM" id="CLU_827783_0_0_1"/>
    <dbReference type="InParanoid" id="F7AFN1"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016051">
        <property type="term" value="P:carbohydrate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22823">
        <property type="entry name" value="Gal_Rha_Lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018011">
        <property type="entry name" value="Carb_sulfotrans_8-10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000922">
        <property type="entry name" value="Lectin_gal-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043159">
        <property type="entry name" value="Lectin_gal-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005331">
        <property type="entry name" value="Sulfotransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12137">
        <property type="entry name" value="CARBOHYDRATE SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12137:SF54">
        <property type="entry name" value="CARBOHYDRATE SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02140">
        <property type="entry name" value="SUEL_Lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03567">
        <property type="entry name" value="Sulfotransfer_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50228">
        <property type="entry name" value="SUEL_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="SUEL-type lectin" evidence="9">
        <location>
            <begin position="44"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000026037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="336" mass="37977" checksum="F189437FCF51856A" modified="2011-07-27" version="1">GKKLCQVVASKAQFLRSTLCDQTPSYLTINYQCLNLNNAEVVTVCQPEIRTIQCQPNYSINILSALYGRIQDTSTCAKGRAIYNLQCSSDTATASVSSACNNQNSCQIQATAKWVQSDGCPATSKYLRVWYVCKPDKITRRRRAAEAPRKKHLKLIKPEDPLKKDTTDVTTGAAALEPPDIYKQPVYIYRHLPLHATFDEFVAYLIDPANKDVTSDNIYHGKETLKSTSVRHWQSFLTLCHPCHIKYDVIAHMDTIAEDSRYILKRIGAPEDLEYPKGYHSTGASGTSIHSMSQHFSQLSLNAIQQLYKLYKPDFDLFGFPEPNEVKLVNEFLKNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>F7AIW6</accession>
    <name>F7AIW6_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">not5</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="F7AIW6"/>
    <dbReference type="Ensembl" id="ENSCINT00000005387.3">
        <property type="protein sequence ID" value="ENSCINP00000005387.3"/>
        <property type="gene ID" value="ENSCING00000002648.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000003689"/>
    <dbReference type="HOGENOM" id="CLU_725532_0_0_1"/>
    <dbReference type="InParanoid" id="F7AIW6"/>
    <dbReference type="OMA" id="AMMADYS"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="56"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="107"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="171"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="213"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="244"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="327"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="62"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="114"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="223"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000005387.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
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<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="10">wild type</strain>
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    <reference evidence="10" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="10" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <text evidence="1 5">Belongs to the importin alpha family.</text>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
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                <person name="Doyle S."/>
                <person name="Francino P."/>
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                <person name="Hayashi H."/>
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                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
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                <person name="Yoshizaki F."/>
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                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
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                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
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    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4">wild type</strain>
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    <reference evidence="4" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="4" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome 5"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000030140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="121" mass="15098" checksum="B05EC3B4BBF83BC8" modified="2012-04-18" version="1">MERTTKIAVIFLLLQLVDLGSALRKVPITRRRFIYYRRRYLPYYYHTNWNTWNTWYTWNNLHYCRRRYYYKVGDQAPTEEHKLNEETMNEKMADFDEFMKQHAQEKSNDLESDALITFYFL</sequence>
</entry>
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    <accession>H2XKK8</accession>
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            <fullName evidence="9">Mitochondrial fission factor</fullName>
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    <reference evidence="13" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
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                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
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                <person name="Boore J."/>
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                <person name="Doyle S."/>
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                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
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                <person name="Medina M."/>
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                <person name="Mount S."/>
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                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="12">wild type</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="12" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <text evidence="9">Plays a role in mitochondrial and peroxisomal fission. Promotes the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface.</text>
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        <text evidence="1 9">Belongs to the Tango11 family.</text>
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        <property type="component" value="Chromosome 8"/>
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        <source>
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        <source>
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        <source>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">wild type</strain>
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    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="molecule type" value="Genomic_DNA"/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="De Tomaso A."/>
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                <person name="Di Gregorio A."/>
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                <person name="Yamada L."/>
                <person name="Wang H.G."/>
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                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
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                <person name="Yoshizaki F."/>
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    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Yamada L."/>
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                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">wild type</strain>
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    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        </location>
    </feature>
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        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12937"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
        </source>
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    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="gene ID" value="ENSCING00000018973.1"/>
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        <property type="component" value="Chromosome 14"/>
    </dbReference>
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        <property type="entry name" value="CCP"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </feature>
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    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
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                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
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                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01000468">
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="ENSCING00000020924.1"/>
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    <dbReference type="InParanoid" id="H2XN78"/>
    <dbReference type="OMA" id="GMADGSH"/>
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        <property type="component" value="Chromosome 10"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="154"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="21"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="37"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="75"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000031111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    </evidence>
    <sequence length="211" mass="23499" checksum="7A31A8C7D824E573" modified="2012-04-18" version="1">MDEEERKRKLEAGKAKFENFRKKKSSKKRKKDKGASAVSTPRDASNSEEVGAGDSIAVQTGDDTSLRALSPSENSELSTTTAEEISTEEEPGYTMELSAIERIQELEITVSDQQIALQKLTLQNKELHEMNQVQALESSIVERDAIISELRDSVGQTADQVRNLQQLLNKATEAVKNTGGEKGEIIAELIETRKKLDTYQLAITRQEETMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
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            <fullName evidence="1">Calcineurin-like phosphoesterase domain-containing protein</fullName>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000032005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>H2XS68</accession>
    <name>H2XS68_CIOIN</name>
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            <fullName evidence="17">ADP/ATP translocase</fullName>
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        <alternativeName>
            <fullName evidence="17">ADP,ATP carrier protein</fullName>
        </alternativeName>
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    <organism evidence="18 19">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
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            <taxon>Phlebobranchia</taxon>
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            <taxon>Ciona</taxon>
        </lineage>
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    <reference evidence="19" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">wild type</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">ADP:ATP antiporter that mediates import of ADP into the mitochondrial matrix for ATP synthesis, and export of ATP out to fuel the cell. Cycles between the cytoplasmic-open state (c-state) and the matrix-open state (m-state): operates by the alternating access mechanism with a single substrate-binding site intermittently exposed to either the cytosolic (c-state) or matrix (m-state) side of the inner mitochondrial membrane.</text>
    </comment>
    <comment type="function">
        <text evidence="17">Catalyzes the exchange of ADP and ATP across the membrane.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ADP(in) + ATP(out) = ADP(out) + ATP(in)</text>
            <dbReference type="Rhea" id="RHEA:34999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:35000"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>H(+)(in) = H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34979"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3 17">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="17">Membrane</location>
            <topology evidence="17">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002233">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XS68"/>
    <dbReference type="FunCoup" id="H2XS68">
        <property type="interactions" value="130"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000032502"/>
    <dbReference type="Ensembl" id="ENSCINT00000035911.1">
        <property type="protein sequence ID" value="ENSCINP00000032502.1"/>
        <property type="gene ID" value="ENSCING00000022760.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160648"/>
    <dbReference type="HOGENOM" id="CLU_015166_12_0_1"/>
    <dbReference type="InParanoid" id="H2XS68"/>
    <dbReference type="OMA" id="CWATIYK"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005471">
        <property type="term" value="F:ATP:ADP antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140021">
        <property type="term" value="P:mitochondrial ADP transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990544">
        <property type="term" value="P:mitochondrial ATP transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901029">
        <property type="term" value="P:negative regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000002">
        <property type="entry name" value="Putative ADP/ATP translocase 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002113">
        <property type="entry name" value="ADT_euk_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45635">
        <property type="entry name" value="ADP,ATP CARRIER PROTEIN 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45635:SF14">
        <property type="entry name" value="ADP_ATP TRANSLOCASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00927">
        <property type="entry name" value="ADPTRNSLCASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0050">Antiport</keyword>
    <keyword evidence="11 15 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 15 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 16" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="171"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="209"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="268"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="15">
        <location>
            <begin position="6"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="15">
        <location>
            <begin position="111"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="15">
        <location>
            <begin position="211"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00024169"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00045250"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU368008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000032502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="298" mass="32725" checksum="7451B9FBE5D88B39" modified="2012-04-18" version="1">MPFSAKSFAQDLAVGGTAAAISKTIVAPIERVKLLLQVQAASKQIAVEDQYKGIVDCFVRIPKEQGMISFWRGNMANVIRYFPTQALNFAFKDTYKQIFLAGVDKKKQFGRYFLGNLASGGAAGATGLCFVYPLDFARTRLAADVGSGKGREFTGLGNCITTIAKRDGVRGLYQGFLVSIQGIIVYRAAYFGTYDTVKGMLPDPKNTPIIVSWAIAQVVTTGAGILSYPFDTVRRRMMMQSGRAKEDLMYKGTIDCWRKIRQQEGGKAFFKGAFSNILRGTGGALVLVLYDELKNLFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>H2XSA3</accession>
    <name>H2XSA3_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
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        <source>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
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                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
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                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
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                <person name="Branno M."/>
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                <person name="Makabe K.W."/>
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                <person name="Mochizuki Y."/>
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                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
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                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
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                <person name="Sasaki A."/>
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                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
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                <person name="Doggett N."/>
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            <dbReference type="PubMed" id="12481130"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    <sequence length="35" mass="4120" checksum="A94EA564605B4DE6" modified="2012-04-18" version="1">MEIVEEHEQAENNKRKKMEKLCDTSFTIPDVETPV</sequence>
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        <name type="scientific">Ciona intestinalis</name>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 2-5A synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001690">
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="STRING" id="7719.ENSCINP00000033439"/>
    <dbReference type="Ensembl" id="ENSCINT00000031706.1">
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        <property type="gene ID" value="ENSCING00000024111.1"/>
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        <property type="component" value="Chromosome 3"/>
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    </evidence>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
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                <person name="Chapman J."/>
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                <person name="De Tomaso A."/>
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                <person name="Hastings K.E."/>
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                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <location evidence="2">Sarcolemma</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="H2XWJ0"/>
    <dbReference type="FunCoup" id="H2XWJ0">
        <property type="interactions" value="4"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000034024"/>
    <dbReference type="Ensembl" id="ENSCINT00000034515.1">
        <property type="protein sequence ID" value="ENSCINP00000034024.1"/>
        <property type="gene ID" value="ENSCING00000022799.1"/>
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    <dbReference type="GeneTree" id="ENSGT00940000153467"/>
    <dbReference type="HOGENOM" id="CLU_001187_1_2_1"/>
    <dbReference type="InParanoid" id="H2XWJ0"/>
    <dbReference type="OMA" id="ASEFICW"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
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    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0045202">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
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    <dbReference type="GO" id="GO:0008270">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="evidence" value="ECO:0000318"/>
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    <dbReference type="InterPro" id="IPR000433">
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    <dbReference type="InterPro" id="IPR043145">
        <property type="entry name" value="Znf_ZZ_sf"/>
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        <property type="entry name" value="DYSTROPHIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12268">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE KCMF1"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF09068">
        <property type="entry name" value="EF-hand_2"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF09069">
        <property type="entry name" value="EF-hand_3"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00569">
        <property type="entry name" value="ZZ"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS50135">
        <property type="entry name" value="ZF_ZZ_2"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
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    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
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    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
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            <end position="286"/>
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        <location>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
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        <source>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50135"/>
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    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000034024.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
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        <recommendedName>
            <fullName evidence="7">G-protein coupled receptors family 2 profile 2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
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            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">wild type</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="EAAA01002849">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XWN7"/>
    <dbReference type="Ensembl" id="ENSCINT00000033121.1">
        <property type="protein sequence ID" value="ENSCINP00000034071.1"/>
        <property type="gene ID" value="ENSCING00000021271.1"/>
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    <dbReference type="GeneTree" id="ENSGT00940000161534"/>
    <dbReference type="HOGENOM" id="CLU_556596_0_0_1"/>
    <dbReference type="InParanoid" id="H2XWN7"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0004930">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12011">
        <property type="entry name" value="ADHESION G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12011:SF58">
        <property type="entry name" value="ADHESION G-PROTEIN COUPLED RECEPTOR D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00249">
        <property type="entry name" value="GPCRSECRETIN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
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    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="30"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="68"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="129"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="163"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="7">
        <location>
            <begin position="1"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="320"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="346"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="356"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000034071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="490" mass="56069" checksum="E8F012A3D6F3E3A1" modified="2012-04-18" version="1">MAWMFVEGIILYRKILIVLTREPQIISKRYYIIGWGVPCLISTLTLIGRTTQNLTYHNERCWLTLQDGIIWTFAGPVCFVIAFNFTILVRVLIVAAATQQRRFLRQQNRYKPTNSNQIQQNIRTVARGIFFLTPLLGLSWVLGFFAATSVVLSYVFVFTNAFQGVYLLLFYVILNPEVKCALLERKRARQRSFLAISGLGSVRTSTQNTWKQRLSFTFLNKLSLSPSTSENTSPVSNELNTQNPKEVQDFNEENLSSRPCSGRSNLNFIISPALRNRGKITPTPERWSDNLKGETNEAFMGDLGNDEYIMSRQTSGIELKKTEDQDDGDLFGNMSDDGGSSLEKLEDTEEYEQELSNEREHVKEEISHPDSESCGYADLTETTEKLEIVQNNRIKTTESKQQEHKQRIKVKQRRKSAPGELVCRSRACHVCKPHRLQHKRKKKKKTEIRAAWPEQASSNNDHCDVMTKMPVLLQNNRPQSTQSRDSGIGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>H2XX32</accession>
    <accession>A0A1W2WEA4</accession>
    <name>H2XX32_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase</fullName>
            <ecNumber evidence="16">2.4.1.135</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">uglcat2l</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">wild type</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11 16">
            <text>3-O-(beta-D-galactosyl-(1-&gt;3)-beta-D-galactosyl-(1-&gt;4)-beta-D-xylosyl)-L-seryl-[protein] + UDP-alpha-D-glucuronate = 3-O-(beta-D-GlcA-(1-&gt;3)-beta-D-Gal-(1-&gt;3)-beta-D-Gal-(1-&gt;4)-beta-D-Xyl)-L-seryl-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24168"/>
            <dbReference type="Rhea" id="RHEA-COMP:12571"/>
            <dbReference type="Rhea" id="RHEA-COMP:12573"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58052"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:132090"/>
            <dbReference type="ChEBI" id="CHEBI:132093"/>
            <dbReference type="EC" id="2.4.1.135"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14 16">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="16">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Homodimer; disulfide-linked. Interacts with PXYLP1; the interaction increases the 2-phosphoxylose phosphatase activity of PXYLP1 during completion of linkage region formation in a B3GAT3-mediated manner.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Golgi apparatus membrane</location>
            <topology evidence="16">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the glycosyltransferase 43 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.135" evidence="16"/>
    <dbReference type="EMBL" id="EAAA01000136">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002127079.1">
        <property type="nucleotide sequence ID" value="XM_002127043.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XX32"/>
    <dbReference type="FunCoup" id="H2XX32">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000034216"/>
    <dbReference type="Ensembl" id="ENSCINT00000035889.1">
        <property type="protein sequence ID" value="ENSCINP00000034216.1"/>
        <property type="gene ID" value="ENSCING00000023722.1"/>
    </dbReference>
    <dbReference type="GeneID" id="100169942"/>
    <dbReference type="KEGG" id="cin:100169942"/>
    <dbReference type="CTD" id="100169942"/>
    <dbReference type="GeneTree" id="ENSGT00940000156954"/>
    <dbReference type="HOGENOM" id="CLU_045177_0_0_1"/>
    <dbReference type="InParanoid" id="H2XX32"/>
    <dbReference type="OMA" id="KGLNYTH"/>
    <dbReference type="OrthoDB" id="675023at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015018">
        <property type="term" value="F:galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050650">
        <property type="term" value="P:chondroitin sulfate proteoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006024">
        <property type="term" value="P:glycosaminoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00218">
        <property type="entry name" value="GlcAT-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000044">
        <property type="entry name" value="Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005027">
        <property type="entry name" value="Glyco_trans_43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10896:SF65">
        <property type="entry name" value="GALACTOSYLGALACTOSYLXYLOSYLPROTEIN 3-BETA-GLUCURONOSYLTRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10896">
        <property type="entry name" value="GALACTOSYLGALACTOSYLXYLOSYLPROTEIN 3-BETA-GLUCURONOSYLTRANSFERASE BETA-1,3-GLUCURONYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03360">
        <property type="entry name" value="Glyco_transf_43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10 14 16" id="KW-0464">Manganese</keyword>
    <keyword evidence="8 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 14 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 16" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 16" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="13">
        <location>
            <position position="230"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="site" description="Interaction with galactose moiety of substrate glycoprotein" evidence="15">
        <location>
            <position position="178"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with galactose moiety of substrate glycoprotein" evidence="15">
        <location>
            <position position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00065147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR605027-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR605027-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR605027-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU363127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000034216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="285" mass="32564" checksum="C3C1A56D1FEE1A3D" modified="2012-04-18" version="1">MVKSTIKLFYTGAVVFVLLIFFIDREILPESVRLLGKGTAEYNDQTIYGITSTYARLTQKADLTRLIYTLMHVPNFHWILIEDSVEKTPLVRRLLQKSGLKYTHLNKKNKKNSHHKSGVKDLLTRNAALAWVRNNVTQGVVYFMDDDNTYDLKLFEEMRTTKVASVWPVGLVGGLVVEGPVRCKNGKVLTWRVTWETNRTIPIDMAGFAINTALLRQHPDVKFVDAFDLESIFLGDLGLTRDKMEAKGNNCREVNVWHTQTAKANLYQEKVAQQLGDEYNPIIET</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>H2XX61</accession>
    <name>H2XX61_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
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                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
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                <person name="Putnam N."/>
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                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
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                <person name="Tassy O."/>
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                <person name="Hyatt P.D."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
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                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Campbell R.K."/>
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                <person name="Hastings K.E."/>
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                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">wild type</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sulfotransferase 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="1"/>
    <dbReference type="EMBL" id="EAAA01001432">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XZN6"/>
    <dbReference type="STRING" id="7719.ENSCINP00000035120"/>
    <dbReference type="Ensembl" id="ENSCINT00000031002.1">
        <property type="protein sequence ID" value="ENSCINP00000035120.1"/>
        <property type="gene ID" value="ENSCING00000024191.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164976"/>
    <dbReference type="HOGENOM" id="CLU_028381_3_0_1"/>
    <dbReference type="InParanoid" id="H2XZN6"/>
    <dbReference type="OMA" id="FWALENY"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001517">
        <property type="term" value="F:N-acetylglucosamine 6-O-sulfotransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006044">
        <property type="term" value="P:N-acetylglucosamine metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006790">
        <property type="term" value="P:sulfur compound metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051135">
        <property type="entry name" value="Gal/GlcNAc/GalNAc_ST"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10704">
        <property type="entry name" value="CARBOHYDRATE SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10704:SF44">
        <property type="entry name" value="LD35051P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003578270" description="Sulfotransferase" evidence="2">
        <location>
            <begin position="22"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="domain" description="Sulfotransferase" evidence="3">
        <location>
            <begin position="87"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU361155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000035120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="402" mass="46146" checksum="F0E0AB5D9A58516E" modified="2012-04-18" version="1">MIVICLSAICVLLWENRTLRCRNVSVSHRLRSPADFPYDEPTVNHTTKPKTRLVNSKTTLVTTPVAITEATATLVHPLQEKPLSKPTAVIIFTSMRSGSSFLGELFNQHSQVFYMFEPLHAFGSYKEQRENRFRALHDISRCNFTNLPNILPYVKSLQFQPTGRRRICAGSVISVTPISANRLGRGVRSRLTHRYCPKFVNEVCFQYPSYVNRIQVIAEPFLESLEPILEDSRVELRVIHLVRDPRAVMSSRKDVFSDAKNTFAIKGNVNYLCNSQRRNLRFAECMNSLCDGVKPSRLWKGRYKRLRFEDVSARPLEVAREVYNFVGLNFNSIIEKWIKLNTRAEASQSSNKFSTKRDSMSAVGHWRQSLSLNETLIIQSACFDNMSKLNYTLISNEHDLNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>H2XZR5</accession>
    <name>H2XZR5_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">wild type</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="EAAA01002452">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XZR5"/>
    <dbReference type="Ensembl" id="ENSCINT00000036269.1">
        <property type="protein sequence ID" value="ENSCINP00000035149.1"/>
        <property type="gene ID" value="ENSCING00000024389.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000014242"/>
    <dbReference type="HOGENOM" id="CLU_1781615_0_0_1"/>
    <dbReference type="InParanoid" id="H2XZR5"/>
    <dbReference type="OMA" id="NLHCRPP"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051547">
        <property type="entry name" value="TDP2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15822">
        <property type="entry name" value="TRAF AND TNF RECEPTOR-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15822:SF4">
        <property type="entry name" value="TYROSYL-DNA PHOSPHODIESTERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000035149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="146" mass="17328" checksum="F20B86F07626FC65" modified="2012-04-18" version="1">MEPAFCCKILSWNLHCRPPHTRLHKNRTERIENVCQTIQRENPTLVSLQEVGVYEYNKIHNHFVNIYGFHSVKSSIGFVEDLDFCITVVMFKKDVVFHYSGNHYKFHSLRMCEYLHVFQGKVKGNEFVLFTSQLEKRKSSSKERQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>H2Y0K1</accession>
    <accession>A0A1W2WK73</accession>
    <name>H2Y0K1_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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                <person name="De Tomaso A."/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
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                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
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                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
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                <person name="Makabe K.W."/>
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                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
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                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="6" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">wild type</strain>
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    <reference evidence="6" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="6" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <location evidence="1">Microtubule organizing center</location>
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        <source>
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</entry>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Di Gregorio A."/>
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                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
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                <person name="Keys D.N."/>
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                <person name="Hayashi H."/>
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                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
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                <person name="Miura S."/>
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                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
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                <person name="Sasakura Y."/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
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                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
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                <person name="Ho I."/>
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                <person name="Huang W."/>
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                <person name="Lemaire P."/>
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                <person name="Necula S."/>
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                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
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                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
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                <person name="Yoshizaki F."/>
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                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
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                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Hastings K.E."/>
                <person name="Ho I."/>
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                <person name="Kawashima T."/>
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                <person name="Putnam N."/>
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                <person name="Satoh N."/>
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        <citation type="submission" date="2005-04" db="EMBL/GenBank/DDBJ databases">
            <title>Expressed genes in Ciona intestinalis.</title>
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                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00504"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000024582.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="409" mass="44747" checksum="4FED4A79D5432421" modified="2017-06-07" version="1">MMNSKRSPAVNRRSPAPSFQTPRPYSMYVSSRHLKTSQDNISLTSSSSSSSFSEVHSTNGGNQTLDSKQKRNSSAELLSTPDCSGVINGLVCGNRTLDVYENSSAELTHSRSIQQKKKKKSPSKRLAVMQVAGPNTRTWLTPKTIKRLATPGPVFGTCISKSGNCYVSVVSIKGERLIQAYDKNCNLVKSFPTRATKGGKVFEPYQIYEMPDDNLAVACGNCVTVWTKEGKLVVEHGRNLFKFAKFLAVRSTGEIVVADSDGDSVKIISAGGQQINKLNCDFKGPTGVAVDSNDNIIVADWKDQIRIFDTDNVLVRSFGHKGDGDGEFDLPYGISVDCEDNIVISDMWNDRVSIYTSEGQFIKHIVPTAGISQILRRPAFISVSCGKIPEDNRLAVTDFLASAVRVFSY</sequence>
</entry>
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    <accession>A0A1W5BHL0</accession>
    <accession>F6XML9</accession>
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        <submittedName>
            <fullName evidence="3">Uncharacterized LOC100182009</fullName>
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        <name evidence="3" type="primary">LOC100182009</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1W5BHL0"/>
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        <property type="protein sequence ID" value="ENSCINP00000005729.3"/>
        <property type="gene ID" value="ENSCING00000002807.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100182009"/>
    <dbReference type="KEGG" id="cin:100182009"/>
    <dbReference type="eggNOG" id="KOG4650">
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    </dbReference>
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    </dbReference>
    <dbReference type="GO" id="GO:0016020">
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        <property type="evidence" value="ECO:0000318"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="UstE-like"/>
    </dbReference>
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        <property type="entry name" value="3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32251:SF17">
        <property type="entry name" value="STEROID 5-ALPHA REDUCTASE C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DUF1295"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="S5A_REDUCTASE"/>
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    </dbReference>
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        <location>
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            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000005729.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="256" mass="29435" checksum="5A5967C5A1EF5104" modified="2017-06-07" version="1">MNNLLTVTLLDFTIQLVGFAVAFALQTEKFYDLTGSCTFILVAQASLRWRANRQFRRVLLSTMVTVWAARLGLFLFYRVLHEGGDKRFNKVKSKPVTFLIYWMIQGVWIIVMSAPVLFVNNERQNVPINTRDYVGFAVWLFGMFFEIMADLQKLTFRNNPDNAGKFISSGLWSISRHPNYFGEIVLWVGVFIIASSDLTGWTWVSILSPVFLYYLLNNVSGVPILERSGLKRYGELPEYKQYINTVPVLVPFVGRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-06-07" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
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    </protein>
    <organism evidence="10 11">
        <name type="scientific">Ciona intestinalis</name>
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    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
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                <person name="Kobayashi M."/>
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                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
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                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
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                <person name="Shoguchi E."/>
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                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
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                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
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                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in the biogenesis of the 60S ribosomal subunit. May play a part in the quality control of pre-60S particles.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 8">Component of the pre-66S ribosomal particle.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
            <location evidence="1 8">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the eukaryotic ribosomal protein eS8 family. Ribosome biogenesis protein NSA2 subfamily.</text>
    </comment>
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    <dbReference type="STRING" id="7719.ENSCINP00000036096"/>
    <dbReference type="Ensembl" id="ENSCINT00000031144.1">
        <property type="protein sequence ID" value="ENSCINP00000036096.1"/>
        <property type="gene ID" value="ENSCING00000024101.1"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3163">
        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="GeneTree" id="ENSGT00390000018706"/>
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    <dbReference type="OMA" id="TNTPEND"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0000460">
        <property type="term" value="P:maturation of 5.8S rRNA"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000470">
        <property type="term" value="P:maturation of LSU-rRNA"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd11381">
        <property type="entry name" value="NSA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.310:FF:000001">
        <property type="entry name" value="NSA2, ribosome biogenesis homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039411">
        <property type="entry name" value="NSA2_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022309">
        <property type="entry name" value="Ribosomal_Se8/biogenesis_NSA2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12642">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN NSA2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01201">
        <property type="entry name" value="Ribosomal_S8e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 8" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="5 8" id="KW-0698">rRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="51"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="51"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="66"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00068584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU367114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000036096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="260" mass="30093" checksum="46EF9E9DDCD3C9C2" modified="2017-06-07" version="1">MPQNEHIELHRKRHGYRLDFHERKRKKEGRMAHDRAKKAKKLIGLKAKLYNKQRHHEKIQMKKTLKMHDKRNTKQKDNEKTPDGAVPAYLLDRESQSRAKVLSNMIKQKRKEKAGKWDVPIPKVRAQSEAEVFRVVTTGKRKKKAWKRMCTKVCFVPDNFTRKPPKYERFIRPMALRFKKAHVTHPELKATFCLPVIGVKKNPSSPMYTSLGVITKGTVIEINVSELGLVTQGGKVIWGKYAQVTNNPENDGCINAVLLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>F6PLR0</accession>
    <name>F6PLR0_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized LOC100178878</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">gnrh1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GnRH family.</text>
    </comment>
    <dbReference type="RefSeq" id="NP_001027799.2">
        <property type="nucleotide sequence ID" value="NM_001032627.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6PLR0"/>
    <dbReference type="STRING" id="7719.ENSCINP00000009883"/>
    <dbReference type="Ensembl" id="ENSCINT00000009883.3">
        <property type="protein sequence ID" value="ENSCINP00000009883.3"/>
        <property type="gene ID" value="ENSCING00000004780.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100178878"/>
    <dbReference type="KEGG" id="cin:100178878"/>
    <dbReference type="CTD" id="2796"/>
    <dbReference type="GeneTree" id="ENSGT00390000005818"/>
    <dbReference type="HOGENOM" id="CLU_1261099_0_0_1"/>
    <dbReference type="InParanoid" id="F6PLR0"/>
    <dbReference type="OMA" id="RQHWSYE"/>
    <dbReference type="OrthoDB" id="8490433at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005179">
        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002012">
        <property type="entry name" value="GnRH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019792">
        <property type="entry name" value="Gonadoliberin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10522">
        <property type="entry name" value="GONADOLIBERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10522:SF5">
        <property type="entry name" value="PREPROGONADOTROPIN-RELEASING HORMONE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00473">
        <property type="entry name" value="GNRH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0027">Amidation</keyword>
    <keyword evidence="4" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="5" id="KW-0372">Hormone</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="125"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="159"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000009883.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="219" mass="24989" checksum="E44204D243692920" modified="2012-04-18" version="2">MLDIEKDELAALLQRENSAFRDILYHKNAGSFEKSDSGKFDSLKLQNNFPHLDLGLGVDLDAVDQWNRYKQANAQRMQNLGVPVNARQHWSYEFMPGGRRAVWENANVGVPVSRQHWSYEYMPGGRRSAGQHAMTKRQHWSKGYSPGGKRSVDLSEFDDQGRRITKHEGMPEEPFKVEQPRPRNGIHGPAGLDQNEPDWKNWMNEQPAASSDDKGSDVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>F6PZN7</accession>
    <name>F6PZN7_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">wild type</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002312">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6PZN7"/>
    <dbReference type="STRING" id="7719.ENSCINP00000011860"/>
    <dbReference type="Ensembl" id="ENSCINT00000011860.3">
        <property type="protein sequence ID" value="ENSCINP00000011860.3"/>
        <property type="gene ID" value="ENSCING00000005742.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000169464"/>
    <dbReference type="HOGENOM" id="CLU_000288_7_35_1"/>
    <dbReference type="InParanoid" id="F6PZN7"/>
    <dbReference type="OMA" id="VMAYAEC"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019899">
        <property type="term" value="F:enzyme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009967">
        <property type="term" value="P:positive regulation of signal transduction"/>
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        <property type="entry name" value="Mitogen-activated protein kinase kinase kinase 7"/>
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        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="1.10.510.10">
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        <property type="entry name" value="Prot_kinase_dom"/>
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    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
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        <property type="entry name" value="Ser/Thr_kinase_AS"/>
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        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 7"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR46716:SF1">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 7"/>
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    </dbReference>
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        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
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    </dbReference>
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    </dbReference>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
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    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="23"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50011"/>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000011860.3"/>
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    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="190" mass="21272" checksum="CAFC864405BF7AFD" modified="2012-04-18" version="2">MAAVTPVIERHPGFIEEIDYNEMELKEVVGKGSFGVVYLAIWRNIQVAVKMIESESERIAFMTELRQLSRVCHPNIIRLYGACRNPVSLVMEFAECGSLYNLLHGPGNQPHYTSGHAMSWCLQCATGVQYLHNMKPKALIHRDLKPPNLLLTNNGTVLKICDFGTACDQHTHMTNNKGSAAWMAPEVFEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>F6Q2J0</accession>
    <name>F6Q2J0_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">wild type</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01002682">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6Q2J0"/>
    <dbReference type="FunCoup" id="F6Q2J0">
        <property type="interactions" value="553"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000013326"/>
    <dbReference type="Ensembl" id="ENSCINT00000013326.3">
        <property type="protein sequence ID" value="ENSCINP00000013326.3"/>
        <property type="gene ID" value="ENSCING00000006476.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157026"/>
    <dbReference type="HOGENOM" id="CLU_004253_14_2_1"/>
    <dbReference type="InParanoid" id="F6Q2J0"/>
    <dbReference type="OMA" id="DRWTIVT"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031463">
        <property type="term" value="C:Cul3-RING ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990756">
        <property type="term" value="F:ubiquitin-like ligase-substrate adaptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18457">
        <property type="entry name" value="BACK_KLHL18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.420:FF:000001">
        <property type="entry name" value="Kelch-like family member 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BACK"/>
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        <property type="entry name" value="BTB-kelch_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
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        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
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        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
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    <dbReference type="PANTHER" id="PTHR24412">
        <property type="entry name" value="KELCH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412:SF497">
        <property type="entry name" value="KELCH-LIKE PROTEIN 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Kelch-like_protein_gigaxonin"/>
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        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="BTB"/>
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        <property type="match status" value="6"/>
    </dbReference>
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        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BTB" evidence="3">
        <location>
            <begin position="41"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000013326.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="577" mass="65101" checksum="847EFC0DB95824B1" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>F6Q5U4</accession>
    <name>F6Q5U4_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Prenyltransferase alpha-alpha toroid domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">wild type</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein prenyltransferase subunit beta family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001880">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="F6Q5U4">
        <property type="interactions" value="63"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000018186"/>
    <dbReference type="Ensembl" id="ENSCINT00000018186.3">
        <property type="protein sequence ID" value="ENSCINP00000018186.3"/>
        <property type="gene ID" value="ENSCING00000008959.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183128"/>
    <dbReference type="HOGENOM" id="CLU_028946_2_2_1"/>
    <dbReference type="InParanoid" id="F6Q5U4"/>
    <dbReference type="OMA" id="CHKTFLP"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005953">
        <property type="term" value="C:CAAX-protein geranylgeranyltransferase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008318">
        <property type="term" value="F:protein prenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045089">
        <property type="entry name" value="PGGT1B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001330">
        <property type="entry name" value="Prenyltrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11774">
        <property type="entry name" value="GERANYLGERANYL TRANSFERASE TYPE BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11774:SF4">
        <property type="entry name" value="GERANYLGERANYL TRANSFERASE TYPE-1 SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00432">
        <property type="entry name" value="Prenyltrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0637">Prenyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Prenyltransferase alpha-alpha toroid" evidence="8">
        <location>
            <begin position="3"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000018186.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="250" mass="27946" checksum="823BDDE17943D08B" modified="2012-04-18" version="2">XEHDCSHIAMTYTGLACLLILGDDLRRVNKHACLEGIRALQLPDGSFKSTYDGSENDMRFIYCACCVCAMLNDFSPINQETATKFIMKSLCYDGAFGQGPGHESHGGSTFCACASLQLMGKLDTALTLKQMKRLQFWCINRQCRGFNGRPHKDDDTCYSFWVGGSLKILNIFDLVDFKENEDFVLSTQDIIVGGFAKWPQVHPADALHSYMGVCGLSLMGLHDLQPIDPTLNITKRAKQHMLSLHQQWVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>F6QH26</accession>
    <name>F6QH26_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">SCP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CRISP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="F6QH26"/>
    <dbReference type="STRING" id="7719.ENSCINP00000004942"/>
    <dbReference type="Ensembl" id="ENSCINT00000004942.3">
        <property type="protein sequence ID" value="ENSCINP00000004942.3"/>
        <property type="gene ID" value="ENSCING00000002429.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165467"/>
    <dbReference type="HOGENOM" id="CLU_539222_0_0_1"/>
    <dbReference type="InParanoid" id="F6QH26"/>
    <dbReference type="OMA" id="RECNTFV"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.33.10:FF:000045">
        <property type="entry name" value="GLIPR1-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018244">
        <property type="entry name" value="Allrgn_V5/Tpx1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052065">
        <property type="entry name" value="Compl_asym_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001283">
        <property type="entry name" value="CRISP-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002413">
        <property type="entry name" value="V5_allergen-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22906">
        <property type="entry name" value="PROPERDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22906:SF43">
        <property type="entry name" value="PROPERDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00090">
        <property type="entry name" value="TSP_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00838">
        <property type="entry name" value="V5ALLERGEN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00837">
        <property type="entry name" value="V5TPXLIKE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00198">
        <property type="entry name" value="SCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="PR-1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01009">
        <property type="entry name" value="CRISP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01010">
        <property type="entry name" value="CRISP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003339884" description="SCP domain-containing protein" evidence="7">
        <location>
            <begin position="25"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="domain" description="SCP" evidence="8">
        <location>
            <begin position="69"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000004942.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="506" mass="55566" checksum="656FBEE0D9E97041" modified="2012-04-18" version="1">KMAWILCFCCLFVTSLLHDVTVNASQTGSEDADVRWSIDAPFTSPSSPNNLAPGSLNSLPGTTVALASSHRDVLVNAHNQARRTTSPTATNMLKMTWDNELAVLAANYARKCLFAHNRTQDRSHSRFHPVGENIYISSGKAFNTRYGSDAVRDWNNEKVDYNYQTRTCTPNRMCGHYTQVVWAETFKVGCGVATCPTVNVRGNRWTDATILICNYGPGGNYINSAPFESGATCSNCHSSDSCENKLCTNRGRDALNRPAASWLSWTPWTTCSKTCGYVGTRRRSRECNTFVNKDCGNKATEFDFTCASIPCGGTTTSGSRWNSWTQWTTCPVTCGGGRKTRERTCTTGRIQDCDGPNQQLLSCNRRPCPNWNSWRQWGSCSETCGSGRRRRSRSCNTGTDSDCVGTSESSDVCNTNVCPTGWSAWEPWGRCSHSCNRGIQLRRRRCLSLSSTDCPGSPSQTQACNSQSCTSQQAVWSNWSAWSACSTSCGNSVSTSTRTCSQTNAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>F6QXW9</accession>
    <name>F6QXW9_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Helicase ATP-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">wild type</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DEAD box helicase family. DEAH subfamily. FANCM sub-subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01000710">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="EAAA01000711">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6QXW9"/>
    <dbReference type="STRING" id="7719.ENSCINP00000006094"/>
    <dbReference type="Ensembl" id="ENSCINT00000006094.3">
        <property type="protein sequence ID" value="ENSCINP00000006094.3"/>
        <property type="gene ID" value="ENSCING00000002994.3"/>
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    <dbReference type="GeneTree" id="ENSGT00940000156480"/>
    <dbReference type="HOGENOM" id="CLU_079193_0_0_1"/>
    <dbReference type="InParanoid" id="F6QXW9"/>
    <dbReference type="OMA" id="RQNIWNA"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="CDD" id="cd18033">
        <property type="entry name" value="DEXDc_FANCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000861">
        <property type="entry name" value="Fanconi anemia, complementation group M"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="1.20.1320.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044749">
        <property type="entry name" value="FANCM_DEXDc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006935">
        <property type="entry name" value="Helicase/UvrB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14025">
        <property type="entry name" value="FANCONI ANEMIA GROUP M FANCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14025:SF20">
        <property type="entry name" value="FANCONI ANEMIA GROUP M PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04851">
        <property type="entry name" value="ResIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="8">
        <location>
            <begin position="27"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000006094.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="273" mass="31164" checksum="2C41269FC126DE84" modified="2012-04-18" version="2">MDSFDKSAGNIWIYPTNYPVRDYQLSITKTALFKNTLVVLPTGLGKTFIASVVMYNFYRWFPQGKIIFIAPTKPLVAQQSKACYDIMGIPADDQAEMTGSSMQVKKRQDLWTNKRVFFLTPQLFNNDLASGTCPACDIKCVVFDEAHKAQGNYAYCQVIQKILVHSKNFRVLALSATPGSNMFAVQQVITNLLISTTEIRSEDAIDVRQYTHTRTVEKVVVKLCDRIMMFKEKYLAVIQVLINRLVSNDILYHKDAKNITKYALLMARDQFRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>F6R501</accession>
    <accession>A0A1W2W5M2</accession>
    <name>F6R501_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">SPARC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100176681</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">wild type</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="EAAA01001098">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002120949.1">
        <property type="nucleotide sequence ID" value="XM_002120913.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6R501"/>
    <dbReference type="STRING" id="7719.ENSCINP00000004847"/>
    <dbReference type="Ensembl" id="ENSCINT00000004847.3">
        <property type="protein sequence ID" value="ENSCINP00000004847.3"/>
        <property type="gene ID" value="ENSCING00000002369.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100176681"/>
    <dbReference type="KEGG" id="cin:100176681"/>
    <dbReference type="GeneTree" id="ENSGT00510000046787"/>
    <dbReference type="HOGENOM" id="CLU_093880_0_0_1"/>
    <dbReference type="InParanoid" id="F6R501"/>
    <dbReference type="OMA" id="PKAKCTE"/>
    <dbReference type="OrthoDB" id="88467at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005518">
        <property type="term" value="F:collagen binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050840">
        <property type="term" value="F:extracellular matrix binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00252">
        <property type="entry name" value="EFh_SPARC_EC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00104">
        <property type="entry name" value="KAZAL_FS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.30:FF:000078">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000246">
        <property type="entry name" value="Uncharacterized protein, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002350">
        <property type="entry name" value="Kazal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036058">
        <property type="entry name" value="Kazal_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019577">
        <property type="entry name" value="SPARC/Testican_Ca-bd-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13866">
        <property type="entry name" value="SPARC OSTEONECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13866:SF31">
        <property type="entry name" value="SPARC-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07648">
        <property type="entry name" value="Kazal_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10591">
        <property type="entry name" value="SPARC_Ca_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00280">
        <property type="entry name" value="KAZAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100895">
        <property type="entry name" value="Kazal-type serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51465">
        <property type="entry name" value="KAZAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014089974" evidence="7">
        <location>
            <begin position="25"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="Kazal-like" evidence="9">
        <location>
            <begin position="73"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="8">
        <location>
            <begin position="167"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000004847.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="251" mass="29197" checksum="F571BE55CA20A457" modified="2012-04-18" version="2">MNVKTVILAVLVVGICTLCGTAEAKKRKRSQQAKLDLLPDEKLCARKRCGRKPKGSWCQVVRDENGHRTPTCVCPRSCNTTQIEPVCSMYGRQYDSECLMHLEACKKRKNIKLAYEGPCIASQAKCQSHELQQFPFRLLDWFVHLKDVDEFGSVDYQKTVMSLSETDRKDVAQWKFQHLDRNKDGKLSNKEIKRFRFALMPLEHCAKHFFKKCDLDKNRKLSSDEWSECLVTRALTWYQGRNEVNREESIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>F6R6I1</accession>
    <name>F6R6I1_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">wild type</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="EAAA01000470">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6R6I1"/>
    <dbReference type="STRING" id="7719.ENSCINP00000018367"/>
    <dbReference type="Ensembl" id="ENSCINT00000018367.3">
        <property type="protein sequence ID" value="ENSCINP00000018367.3"/>
        <property type="gene ID" value="ENSCING00000009050.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157259"/>
    <dbReference type="HOGENOM" id="CLU_000288_63_0_1"/>
    <dbReference type="InParanoid" id="F6R6I1"/>
    <dbReference type="OMA" id="SCCPSDV"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000003">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF42">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE SIK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="29"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000018367.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="201" mass="22937" checksum="E06CB17C2A34467B" modified="2012-04-18" version="2">MTSNGQVGDASCQQNNSHKPPSYVRVGCYEIERTIGKGNFAVVKLATHIQTKAKVAIKIVDKTQLDKENLKKIYREIEIMKELRHPHIIKLYQVMQTENKLFLVTEYASSGEIFDHLVAHGRMAEREARIKFKQIVAAVYYCHSRHVVHRDLKAENLLLDAGKNIKIADFGFANYYKGEDLLKTWCGSPPYAAPELFEGKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>F6RBS2</accession>
    <name>F6RBS2_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Methyltransferase type 11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">wild type</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002970">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6RBS2"/>
    <dbReference type="FunCoup" id="F6RBS2">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSCINT00000024216.2">
        <property type="protein sequence ID" value="ENSCINP00000023970.2"/>
        <property type="gene ID" value="ENSCING00000012960.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163794"/>
    <dbReference type="HOGENOM" id="CLU_049344_5_2_1"/>
    <dbReference type="InParanoid" id="F6RBS2"/>
    <dbReference type="OMA" id="STHAYEM"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051052">
        <property type="entry name" value="Diverse_substrate_MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44942">
        <property type="entry name" value="METHYLTRANSF_11 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44942:SF4">
        <property type="entry name" value="METHYLTRANSFERASE TYPE 11 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="4">
        <location>
            <begin position="46"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000023970.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="274" mass="31447" checksum="0E32993A78BC0C5B" modified="2012-04-18" version="2">RMYRLYESKSLTAAYKKYRPTHPPELARFVVNKMRSKNKTDSSEKVDVGCGSGQATNIFAPYFKKVLATDPSVNQLEHARMQNKFDNVSYEEGTAENIKVEDCSLDLLVAGCALPYFDHPKFLQEADRVLKPGGYMALYGLQVPVLLQPDEVESIGTSIFKSYSKLCLDEFPDIQHLVVFWNTKYQTIFDAIKYEDKERKESFSLVKNSTMEDFLGFFRSTHAYEMYMKKQAVGLNNLSDKEQMEKLKQLDVAEHCADEVLSRCYYCSCATKPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>F6RD62</accession>
    <name>F6RD62_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the G-alpha family. G(12) subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="F6RD62"/>
    <dbReference type="FunCoup" id="F6RD62">
        <property type="interactions" value="37"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000006303"/>
    <dbReference type="Ensembl" id="ENSCINT00000006303.3">
        <property type="protein sequence ID" value="ENSCINP00000006303.3"/>
        <property type="gene ID" value="ENSCING00000003111.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000168398"/>
    <dbReference type="HOGENOM" id="CLU_014184_3_1_1"/>
    <dbReference type="InParanoid" id="F6RD62"/>
    <dbReference type="OMA" id="WVQCFED"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031526">
        <property type="term" value="C:brush border membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005834">
        <property type="term" value="C:heterotrimeric G-protein complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031752">
        <property type="term" value="F:D5 dopamine receptor binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031683">
        <property type="term" value="F:G-protein beta/gamma-subunit complex binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007188">
        <property type="term" value="P:adenylate cyclase-modulating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007266">
        <property type="term" value="P:Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00066">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.400.10:FF:000004">
        <property type="entry name" value="Guanine nucleotide-binding protein subunit alpha-12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000754">
        <property type="entry name" value="Guanine nucleotide-binding protein subunit alpha-13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.400.10">
        <property type="entry name" value="GI Alpha 1, domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000469">
        <property type="entry name" value="Gprotein_alpha_12/13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001019">
        <property type="entry name" value="Gprotein_alpha_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011025">
        <property type="entry name" value="GproteinA_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218">
        <property type="entry name" value="GTP-BINDING PROTEIN ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218:SF360">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN SUBUNIT ALPHA HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00503">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00318">
        <property type="entry name" value="GPROTEINA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00440">
        <property type="entry name" value="GPROTEINA12"/>
    </dbReference>
    <dbReference type="SMART" id="SM00275">
        <property type="entry name" value="G_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47895">
        <property type="entry name" value="Transducin (alpha subunit), insertion domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51882">
        <property type="entry name" value="G_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0564">Palmitate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0807">Transducer</keyword>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="65"/>
            <end position="69"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="197"/>
            <end position="203"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="222"/>
            <end position="226"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="291"/>
            <end position="294"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="349"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000006303.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="377" mass="44416" checksum="3753BAFCC4807001" modified="2012-04-18" version="2">LSGYVTGLSKYMQYSNTDLKENRLSGAMEEQYTNKTKSNAIDRDIAKEKMFYKRQVKLLLLGCGESGKTFLKQMRILHGQGYDDKALLEFRATVYSNIVKGMKVLVDARNKLGIPWEDPSRQSQADYIMTFERYVSTDVDPFLQYVPHIKELWKDEGIHTAYYRRREFQLGESVHYFLENLDRIGQSDYVPSMQDVLLSRQATRGIQEYVFEIRRIPFRFFDVGGQRGQRQKWLKCFEDVTSILFLASSSEFDQVLMEDRKTNRLQESLDIFELIVNNRYFVNVSIILFLNKSDLLEEKVKQGVLLSDYFPDFEGDPLDLSDVQNYLVYKFESQRRDRGKPFFHHFTMAIDTNNIRFVFNAVKDTILHDNLHQLMLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>F6RLP1</accession>
    <name>F6RLP1_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cilia- and flagella-associated protein 45</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
            <location evidence="1">Flagellum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the CFAP45 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="F6RLP1"/>
    <dbReference type="FunCoup" id="F6RLP1">
        <property type="interactions" value="6"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000023931"/>
    <dbReference type="Ensembl" id="ENSCINT00000024177.2">
        <property type="protein sequence ID" value="ENSCINP00000023931.2"/>
        <property type="gene ID" value="ENSCING00000012929.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00730000111174"/>
    <dbReference type="HOGENOM" id="CLU_026959_1_1_1"/>
    <dbReference type="InParanoid" id="F6RLP1"/>
    <dbReference type="OMA" id="WGHKPET"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033253">
        <property type="entry name" value="CFAP45"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043597">
        <property type="entry name" value="TPH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15504:SF0">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15504">
        <property type="entry name" value="NASOPHARYNGEAL EPITHELIUM SPECIFIC PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13868">
        <property type="entry name" value="TPH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0969">Cilium</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0282">Flagellum</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Trichohyalin-plectin-homology" evidence="9">
        <location>
            <begin position="181"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="129"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="391"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="7"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000023931.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="546" mass="64721" checksum="779639CEBC5A7B87" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>F6RXY6</accession>
    <name>F6RXY6_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="22">Laminin subunit alpha-5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">LOC100176802</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">wild type</strain>
        </source>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
            <location evidence="1">Basement membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001429">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="FunCoup" id="F6RXY6">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000010645"/>
    <dbReference type="Ensembl" id="ENSCINT00000010645.3">
        <property type="protein sequence ID" value="ENSCINP00000010645.3"/>
        <property type="gene ID" value="ENSCING00000005180.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000167067"/>
    <dbReference type="HOGENOM" id="CLU_000301_1_0_1"/>
    <dbReference type="InParanoid" id="F6RXY6"/>
    <dbReference type="OMA" id="ISHCAAH"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005604">
        <property type="term" value="C:basement membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005201">
        <property type="term" value="F:extracellular matrix structural constituent"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009887">
        <property type="term" value="P:animal organ morphogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030155">
        <property type="term" value="P:regulation of cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030334">
        <property type="term" value="P:regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045995">
        <property type="term" value="P:regulation of embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009888">
        <property type="term" value="P:tissue development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00055">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="21"/>
    </dbReference>
    <dbReference type="CDD" id="cd00110">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000011">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000074">
        <property type="entry name" value="Laminin subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000083">
        <property type="entry name" value="Laminin subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000090">
        <property type="entry name" value="laminin subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000069">
        <property type="entry name" value="Laminin subunit alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000082">
        <property type="entry name" value="Laminin subunit alpha 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000051">
        <property type="entry name" value="Laminin subunit alpha 4"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <sequence length="3767" mass="410037" checksum="CE7A463E75321197" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>F6SDM6</accession>
    <name>F6SDM6_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Zinc finger protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
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</entry>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
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                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
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                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="2" key="3">
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                <consortium name="Ensembl"/>
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            <begin position="35"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000000706.3"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="135" mass="15077" checksum="6E2016FC2F7416D1" modified="2012-04-18" version="2">MVLRNTTTAFSGNSSNATISTFVTTPSSGSIDPTVLALFISISSIVLFFIFITCMLSLSRKKKNKFTPGNNLEEHQAPPHPYPQYNYEGNAAELVVVKEHQSSIIIGNKIRTRHEETTFNSNRIQQQSHSLTYKV</sequence>
</entry>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="common">Transparent sea squirt</name>
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        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01002785">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6SH76"/>
    <dbReference type="Ensembl" id="ENSCINT00000024403.2">
        <property type="protein sequence ID" value="ENSCINP00000024157.2"/>
        <property type="gene ID" value="ENSCING00000013092.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000064870"/>
    <dbReference type="HOGENOM" id="CLU_1258628_0_0_1"/>
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    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="53"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="112"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="149"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="201"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000024157.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="220" mass="24729" checksum="742374C5BB8B5F29" modified="2011-07-27" version="1">MFIIICGNIGLLIDSIRHTTCLTLRTAAGYRSHQSEGNGGRWKKRLLTRRQRSYILIAVLWIVPIIYVLIPIAGWSCAEICQCLSQCFSPDHEFQPSDYRCSRAFPPMANSWMALVVGAWGCLLLGVIYFLKRSIQQVNEMWSRCHSNRSSERKNTDASSGSPDSVRGISKRNGSRNSYVMNRMSNDVTCSKDEAICSRGRVNSSSGAHHDRLPSCFDSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>F6SRF9</accession>
    <name>F6SRF9_CIOIN</name>
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        <recommendedName>
            <fullName evidence="3">2'-phosphotransferase</fullName>
            <ecNumber evidence="3">2.7.1.160</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100186294</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the last step of tRNA splicing, the transfer of the splice junction 2'-phosphate from ligated tRNA to NAD to produce ADP-ribose 1''-2'' cyclic phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>2'-phospho-[ligated tRNA] + NAD(+) = mature tRNA + ADP-alpha-D-ribose 1'',2''-cyclic phosphate + nicotinamide</text>
            <dbReference type="Rhea" id="RHEA:23324"/>
            <dbReference type="Rhea" id="RHEA-COMP:11106"/>
            <dbReference type="Rhea" id="RHEA-COMP:11107"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:76596"/>
            <dbReference type="ChEBI" id="CHEBI:82883"/>
            <dbReference type="ChEBI" id="CHEBI:85027"/>
            <dbReference type="EC" id="2.7.1.160"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the KptA/TPT1 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.160" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="F6SRF9"/>
    <dbReference type="FunCoup" id="F6SRF9">
        <property type="interactions" value="4"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000028415"/>
    <dbReference type="Ensembl" id="ENSCINT00000028661.2">
        <property type="protein sequence ID" value="ENSCINP00000028415.2"/>
        <property type="gene ID" value="ENSCING00000024241.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000002731"/>
    <dbReference type="HOGENOM" id="CLU_052998_1_1_1"/>
    <dbReference type="InParanoid" id="F6SRF9"/>
    <dbReference type="OMA" id="GRIHPKY"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000215">
        <property type="term" value="F:tRNA 2'-phosphotransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006388">
        <property type="term" value="P:tRNA splicing, via endonucleolytic cleavage and ligation"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.970">
        <property type="entry name" value="RNA 2'-phosphotransferase, Tpt1/KptA family, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002745">
        <property type="entry name" value="Ptrans_KptA/Tpt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042081">
        <property type="entry name" value="RNA_2'-PTrans_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042080">
        <property type="entry name" value="RNA_2'-PTrans_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12684">
        <property type="entry name" value="PUTATIVE PHOSPHOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12684:SF2">
        <property type="entry name" value="TRNA 2'-PHOSPHOTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01885">
        <property type="entry name" value="PTS_2-RNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56399">
        <property type="entry name" value="ADP-ribosylation"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
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    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="10"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00047949"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000028415.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    </evidence>
    <sequence length="243" mass="27973" checksum="CD081A11054A105D" modified="2012-04-18" version="2">YNENKGSSSHSEDKRNRSGERGRDGYHGGGGGGARQNHQRVQVIGKELVRIVRHGKYNQQIQMDAEGYVFVNDILTCKRFYDQFPGVNELYIEKIVESNDKKRFELKFEEGVGWKIKAAQGHTVKIENRSLRPITNPAEFPVVIHGTYWENWEFIKVNGLNRMGRNHIHFAPGLPNEVVSGMRKSCEVAIYINMTQALKDGFKFFQSSNQVILTEGDEKGRIHPKYFLKVVQLYPRKGIKFEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>F6T143</accession>
    <accession>A0A1W3JIN1</accession>
    <name>F6T143_CIOIN</name>
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        <submittedName>
            <fullName evidence="9">MAGUK p55 subfamily member 7</fullName>
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    <gene>
        <name evidence="9" type="primary">LOC100181746</name>
    </gene>
    <organism evidence="9 10">
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        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
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            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">wild type</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM). Has a strong preference for putrescine as substrate, and has very low activity towards 1,3-diaminopropane. Has extremely low activity towards spermidine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>S-adenosyl 3-(methylsulfanyl)propylamine + putrescine = S-methyl-5'-thioadenosine + spermidine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12721"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:57443"/>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
            <dbReference type="ChEBI" id="CHEBI:326268"/>
            <dbReference type="EC" id="2.5.1.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amine and polyamine biosynthesis; spermidine biosynthesis; spermidine from putrescine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer or homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the spermidine/spermine synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.16" evidence="4"/>
    <dbReference type="EMBL" id="EAAA01002506">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6TLL4"/>
    <dbReference type="FunCoup" id="F6TLL4">
        <property type="interactions" value="378"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000019159"/>
    <dbReference type="Ensembl" id="ENSCINT00000019159.3">
        <property type="protein sequence ID" value="ENSCINP00000019159.3"/>
        <property type="gene ID" value="ENSCING00000009423.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00870000136521"/>
    <dbReference type="HOGENOM" id="CLU_048199_1_0_1"/>
    <dbReference type="InParanoid" id="F6TLL4"/>
    <dbReference type="OMA" id="FLYHEMM"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004766">
        <property type="term" value="F:spermidine synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008295">
        <property type="term" value="P:spermidine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.140.10:FF:000001">
        <property type="entry name" value="SPE3p Spermidine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000013">
        <property type="entry name" value="Spermidine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.140.10">
        <property type="entry name" value="Spermidine synthase, tetramerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00198">
        <property type="entry name" value="Spermidine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030374">
        <property type="entry name" value="PABS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030373">
        <property type="entry name" value="PABS_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001045">
        <property type="entry name" value="Spermi_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030668">
        <property type="entry name" value="Spermi_synthase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035246">
        <property type="entry name" value="Spermidine_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037163">
        <property type="entry name" value="Spermidine_synt_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002010">
        <property type="entry name" value="PRK00811.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00417">
        <property type="entry name" value="speE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11558:SF11">
        <property type="entry name" value="SPERMIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11558">
        <property type="entry name" value="SPERMIDINE/SPERMINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17284">
        <property type="entry name" value="Spermine_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01564">
        <property type="entry name" value="Spermine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000502">
        <property type="entry name" value="Spermidine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01330">
        <property type="entry name" value="PABS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51006">
        <property type="entry name" value="PABS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0620">Polyamine biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PABS" evidence="12">
        <location>
            <begin position="11"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10">
        <location>
            <position position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00053963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00072554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00082964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000019159.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="305" mass="34540" checksum="104C32C6ABE509FE" modified="2012-04-18" version="2">YAVYKMDELKLNWFSELNSLWPGQCMSLEVEEVLFHERSKYQDVLVFKSKTYGNVLVLDGVIQCTERDEFAYQEMIAHLPLFSHPNPKNVLVIGGGDGGVIREVLKHSSVVSITQCEIDQVVIDVSRKFLPSMSKSMDDPRVTQFVGDGLAFMREHKAEFDVIITDSSDPQGPAEALFEKPYYQLMQKALKDGGIICAQGECVWLHLSLICNMKKFCETVFPSVAYGYCTIPTYPSGQIGFMLCSQNKETNFKEPCRVLSCDEVNKMELKYYNSDVHKAAFVLPQFAKSVSIGKQLCSTWPIQLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>F6U0I8</accession>
    <name>F6U0I8_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">dihydropyrimidinase</fullName>
            <ecNumber evidence="6">3.5.2.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">wild type</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>5,6-dihydrouracil + H2O = 3-(carbamoylamino)propanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16121"/>
            <dbReference type="ChEBI" id="CHEBI:11892"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15901"/>
            <dbReference type="EC" id="3.5.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="PTM">
        <text evidence="7">Carbamylation allows a single lysine to coordinate two divalent metal cations.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the metallo-dependent hydrolases superfamily. Hydantoinase/dihydropyrimidinase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.2.2" evidence="6"/>
    <dbReference type="EMBL" id="EAAA01001347">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6U0I8"/>
    <dbReference type="FunCoup" id="F6U0I8">
        <property type="interactions" value="41"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000001663"/>
    <dbReference type="Ensembl" id="ENSCINT00000001663.3">
        <property type="protein sequence ID" value="ENSCINP00000001663.3"/>
        <property type="gene ID" value="ENSCING00000000922.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234527"/>
    <dbReference type="HOGENOM" id="CLU_015572_2_2_1"/>
    <dbReference type="InParanoid" id="F6U0I8"/>
    <dbReference type="OMA" id="SAETHHM"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004157">
        <property type="term" value="F:dihydropyrimidinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006208">
        <property type="term" value="P:pyrimidine nucleobase catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01314">
        <property type="entry name" value="D-HYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000076">
        <property type="entry name" value="Dihydropyrimidinase like 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="entry name" value="Urease, subunit C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011778">
        <property type="entry name" value="Hydantoinase/dihydroPyrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050378">
        <property type="entry name" value="Metallo-dep_Hydrolases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02033">
        <property type="entry name" value="D-hydantoinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11647:SF1">
        <property type="entry name" value="COLLAPSIN RESPONSE MEDIATOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11647">
        <property type="entry name" value="HYDRANTOINASE/DIHYDROPYRIMIDINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="Composite domain of metallo-dependent hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="9">
        <location>
            <begin position="49"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="513"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="513"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-carboxylysine" evidence="7">
        <location>
            <position position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00036696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR611778-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000001663.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="544" mass="59704" checksum="ACCFEFF287F585DA" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>F6UBS8</accession>
    <accession>A0A1W2W8T7</accession>
    <name>F6UBS8_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">2-phosphoxylose phosphatase 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Acid phosphatase-like protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100180903</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">wild type</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>3-O-[beta-D-GlcA-(1-&gt;3)-beta-D-Gal-(1-&gt;3)-beta-D-Gal-(1-&gt;4)-beta-D-2-O-P-Xyl]-L-seryl-[protein] + H2O = 3-O-(beta-D-GlcA-(1-&gt;3)-beta-D-Gal-(1-&gt;3)-beta-D-Gal-(1-&gt;4)-beta-D-Xyl)-L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:56512"/>
            <dbReference type="Rhea" id="RHEA-COMP:12573"/>
            <dbReference type="Rhea" id="RHEA-COMP:14559"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:132093"/>
            <dbReference type="ChEBI" id="CHEBI:140495"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the histidine acid phosphatase family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01000601">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002126983.1">
        <property type="nucleotide sequence ID" value="XM_002126947.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6UBS8"/>
    <dbReference type="STRING" id="7719.ENSCINP00000012992"/>
    <dbReference type="Ensembl" id="ENSCINT00000012992.3">
        <property type="protein sequence ID" value="ENSCINP00000012992.3"/>
        <property type="gene ID" value="ENSCING00000006309.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100180903"/>
    <dbReference type="KEGG" id="cin:100180903"/>
    <dbReference type="GeneTree" id="ENSGT00390000016324"/>
    <dbReference type="HOGENOM" id="CLU_033855_1_0_1"/>
    <dbReference type="InParanoid" id="F6UBS8"/>
    <dbReference type="OMA" id="CALGQLT"/>
    <dbReference type="OrthoDB" id="10262962at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050650">
        <property type="term" value="P:chondroitin sulfate proteoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07061">
        <property type="entry name" value="HP_HAP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033379">
        <property type="entry name" value="Acid_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000560">
        <property type="entry name" value="His_Pase_clade-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050645">
        <property type="entry name" value="Histidine_acid_phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11567:SF110">
        <property type="entry name" value="2-PHOSPHOXYLOSE PHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11567">
        <property type="entry name" value="ACID PHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00328">
        <property type="entry name" value="His_Phos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00616">
        <property type="entry name" value="HIS_ACID_PHOSPHAT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="45"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="45"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00036311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00040357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00041499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000012992.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="490" mass="56413" checksum="CC1727A61D9DA967" modified="2011-07-27" version="1">MKKASTKWMLFATALISIFLVTMWTLQYRSKDHFKAKQLNTDHSYKLESEKEQPKTTTTPAPLVPHTQPGVRVKTKKEVKPNPVEYYCNFPNSNDIFKMQEGNGQKGWKLKQVQIVIRHGDRAPLNLNVYHKFRTKNRFCNIHSNFYSDTLKLVSNYRNSIMKDQNSQHLPYFAQPLPEGIVCSPGQLTVIGALQHLHNGLMLREAYIRKHKLVDPATQDKFVYVRSTHVRRTFQSAVAFLYGFLPNYHLQDIPITFTMGPTFCEKHCVCPIVKTLEKKVKKTQYQNYLKRQRNNPIIKNISRITGIGDHTGPLMDSLVTGYICKQIPLPCNENGECVELSELNVVMNEQEYLNRVTRLESPARNISLLQIQPFLENITNHMKQVVEDSAAQRKAVLYSGHDLTLAPLIQALGLPEYRWPRLASRLVFELWQATDGMHFVKILFNGVDVTNDTAFCPGKNKPCSYNSFMQFQRDIGKYFGFDSRSSVCKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>F6UMR8</accession>
    <name>F6UMR8_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">wild type</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="EAAA01002809">
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    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AWS" evidence="12">
        <location>
            <begin position="244"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="SET" evidence="10">
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            <end position="411"/>
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    </feature>
    <feature type="domain" description="Post-SET" evidence="11">
        <location>
            <begin position="418"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
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    <feature type="region of interest" description="Disordered" evidence="9">
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            <begin position="1"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="9">
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            <begin position="130"/>
            <end position="141"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
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            <dbReference type="ARBA" id="ARBA00023242"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="PROSITE" id="PS50280"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    <sequence length="477" mass="53632" checksum="508384D9B46144A4" modified="2012-04-18" version="2">MTANVKQSHPTSLPNHEFIPKPPDFPPNPHEPPKYAISSPESPLFSPGSESPCIAPQMTAFDDASSGTDEMCDPISIRIPVDDHGRVNDTNQNTELEVTGSKMELDVTLTQDNQDAVNDKQANNTEPTIDSVSDASNSAVDETAEGRRRSARIMTKMQKRKSRWSSRPQGVDSPTPEVQNKTENMDLLFDKQQQQQQQQQEEEEDKVKTVGENALSNTASPSFEAISDNIYLCEKKKSRVRKEIRRMVCECDNNEDGTPCGSDCLNRLLMIECSARCPLGEQCQNKRFQRKQYVPTEVFQTKWKGWGIRATENLSPGMLVMEYCGEVLDLQEFGRRSLLYSRGNQQHFYFMALSQDEIIDATTKGNTSRFINHSCDPNCETQKWTVNGRLRVGFFTMRDINKGEEITFDYQFQRYGKEAQACYCGSSNCRGYLGKAPDEDDDVIADDVMANDVTCVDDVSKFEDNSAVITSVATETG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>F6V0K9</accession>
    <accession>A0A1W2W9K2</accession>
    <name>F6V0K9_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Tetraspanin-8-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100179957</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">wild type</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tetraspanin (TM4SF) family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01000454">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002122534.1">
        <property type="nucleotide sequence ID" value="XM_002122498.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6V0K9"/>
    <dbReference type="FunCoup" id="F6V0K9">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000012965"/>
    <dbReference type="Ensembl" id="ENSCINT00000012965.3">
        <property type="protein sequence ID" value="ENSCINP00000012965.3"/>
        <property type="gene ID" value="ENSCING00000006285.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100179957"/>
    <dbReference type="KEGG" id="cin:100179957"/>
    <dbReference type="GeneTree" id="ENSGT00940000165843"/>
    <dbReference type="HOGENOM" id="CLU_055524_4_2_1"/>
    <dbReference type="InParanoid" id="F6V0K9"/>
    <dbReference type="OMA" id="CIDWELE"/>
    <dbReference type="OrthoDB" id="5982705at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000301">
        <property type="entry name" value="Tetraspanin_animals"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282:SF551">
        <property type="entry name" value="RE08073P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002419">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00259">
        <property type="entry name" value="TMFOUR"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="54"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="84"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="202"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="144"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR002419-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000012965.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="236" mass="25451" checksum="9D0F278C6B5CABAB" modified="2012-04-18" version="2">MCCCSISHFIFYLLNTFIWFTGLSTLGIGIWLLVDSDIQQALTVSGLEDYTAGAIVFVVVGSIIFLVGFIGCCGVHNKKSSLKIMYIIFLGGIFAAEIGIGIWALENNTSLESKINSNMNVTGTLLNKTTDSNYKELEQSFECCGATQGCIDWELESKSFGCECNTTSENCVPVPANCLSNSTRIYLQPCSESIYNVLSQNLLVVGGVGIAFSAIEILAIVMTLFLLCCDNKVGSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>F6VF47</accession>
    <accession>A0A1W2WH27</accession>
    <name>F6VF47_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Frizzled-1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100186600</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">wild type</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01000095">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002128955.1">
        <property type="nucleotide sequence ID" value="XM_002128919.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6VF47"/>
    <dbReference type="STRING" id="7719.ENSCINP00000004686"/>
    <dbReference type="Ensembl" id="ENSCINT00000004686.3">
        <property type="protein sequence ID" value="ENSCINP00000004686.3"/>
        <property type="gene ID" value="ENSCING00000010224.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100186600"/>
    <dbReference type="KEGG" id="cin:100186600"/>
    <dbReference type="GeneTree" id="ENSGT00940000166857"/>
    <dbReference type="HOGENOM" id="CLU_126325_1_0_1"/>
    <dbReference type="InParanoid" id="F6VF47"/>
    <dbReference type="OMA" id="IPRCRAM"/>
    <dbReference type="OrthoDB" id="10053709at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042813">
        <property type="term" value="F:Wnt receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017147">
        <property type="term" value="F:Wnt-protein binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060070">
        <property type="term" value="P:canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035567">
        <property type="term" value="P:non-canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07066">
        <property type="entry name" value="CRD_FZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.2000.10:FF:000032">
        <property type="entry name" value="Secreted frizzled-related protein precusor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.2000.10">
        <property type="entry name" value="Frizzled cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015526">
        <property type="entry name" value="Frizzled/SFRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020067">
        <property type="entry name" value="Frizzled_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036790">
        <property type="entry name" value="Frizzled_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11309">
        <property type="entry name" value="FRIZZLED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11309:SF126">
        <property type="entry name" value="FRIZZLED-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01392">
        <property type="entry name" value="Fz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00063">
        <property type="entry name" value="FRI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63501">
        <property type="entry name" value="Frizzled cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50038">
        <property type="entry name" value="FZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="2 3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014090123" evidence="4">
        <location>
            <begin position="23"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="FZ" evidence="5">
        <location>
            <begin position="20"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="25"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="33"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="100"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000004686.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="150" mass="16710" checksum="48977ED4842EDCDE" modified="2012-04-18" version="2">MGSFAIFSLLLVLIVTVQKCKSLNCQEVISPICSDIIRYPVLMPNMFGHTTQDEANIELSQYYPFLKIGCSPYLKPFLCSAFFSPCTSKGTRKLPCRSLCENTMVGCLEVATRFGFVVPEALNCARFPEQTSTSYCIQPESFGLSPIKHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>F6W1C5</accession>
    <name>F6W1C5_CIOIN</name>
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        <recommendedName>
            <fullName evidence="6">WD repeat domain-containing protein 83</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Mitogen-activated protein kinase organizer 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC100187342</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">wild type</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the WD repeat MORG1 family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001970">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6W1C5"/>
    <dbReference type="FunCoup" id="F6W1C5">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000006793"/>
    <dbReference type="Ensembl" id="ENSCINT00000006793.3">
        <property type="protein sequence ID" value="ENSCINP00000006793.3"/>
        <property type="gene ID" value="ENSCING00000003306.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159016"/>
    <dbReference type="HOGENOM" id="CLU_000288_57_1_1"/>
    <dbReference type="InParanoid" id="F6W1C5"/>
    <dbReference type="OMA" id="MCWDIRT"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000273">
        <property type="entry name" value="WD repeat domain-containing protein 83"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051980">
        <property type="entry name" value="WD_repeat_MORG1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22842:SF3">
        <property type="entry name" value="WD REPEAT DOMAIN-CONTAINING PROTEIN 83"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22842">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
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    <dbReference type="SMART" id="SM00320">
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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        <source>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000006793.3"/>
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    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="330" mass="36608" checksum="B2440BCD1E423391" modified="2012-04-18" version="2">MFGVVEGPANRPDAKEEKAPPKAKPRLPANLIHFIDCNQGAVRAVRFNGDGNYCLTCGNDKSIKLWNPEKQMLIKKYTGHGYDVLDAASSNDNCQIASCGSDKSVMYWDVASGQIIRRFRGHAGRVNCVQFNEESTVILSGSVDSSIRAWDCRSKKGSAIQIMDEAKDSISALQVSDHEILSGSIDGKIRRYDLRIGSMHSDNIGSAITSTCFTKDGQCTLTSTHDNTIRLLDVATGEMLGEYMGHKNAKYMIECALNRKDDHVISGSEDGHIYMWDLVEASVTKKLLVKRNKVIHSLSMHPEKDVMLVACENKIYMFADDFYELPDNIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>F6W7L1</accession>
    <name>F6W7L1_CIOIN</name>
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        <recommendedName>
            <fullName evidence="11">Solute carrier family 23 member 2</fullName>
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    </protein>
    <organism evidence="9 10">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
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    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) family.</text>
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    <dbReference type="Ensembl" id="ENSCINT00000002802.3">
        <property type="protein sequence ID" value="ENSCINP00000002802.3"/>
        <property type="gene ID" value="ENSCING00000018372.1"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="Xan_ur_permease"/>
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        <property type="match status" value="1"/>
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    <feature type="transmembrane region" description="Helical" evidence="8">
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    <feature type="transmembrane region" description="Helical" evidence="8">
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        <location>
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        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000002802.3"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="600" mass="65484" checksum="0421D02327F967CA" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
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            <fullName evidence="11">Transcription factor protein</fullName>
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        <name type="common">Transparent sea squirt</name>
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        <lineage>
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            <taxon>Phlebobranchia</taxon>
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            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
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                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the E2F/DP family.</text>
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    <dbReference type="AlphaFoldDB" id="F6WIW4"/>
    <dbReference type="STRING" id="7719.ENSCINP00000003677"/>
    <dbReference type="Ensembl" id="ENSCINT00000003677.3">
        <property type="protein sequence ID" value="ENSCINP00000003677.3"/>
        <property type="gene ID" value="ENSCING00000001818.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000169233"/>
    <dbReference type="HOGENOM" id="CLU_039874_0_0_1"/>
    <dbReference type="InParanoid" id="F6WIW4"/>
    <dbReference type="OMA" id="NDKYEYM"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
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    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14458">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.80:FF:000001">
        <property type="entry name" value="Transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.80">
        <property type="entry name" value="Transcription factor DP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037241">
        <property type="entry name" value="E2F-DP_heterodim"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003316">
        <property type="entry name" value="E2F_WHTH_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038168">
        <property type="entry name" value="TF_DP_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014889">
        <property type="entry name" value="Transc_factor_DP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015648">
        <property type="entry name" value="Transcrpt_fac_DP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12548">
        <property type="entry name" value="TRANSCRIPTION FACTOR DP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12548:SF9">
        <property type="entry name" value="TRANSCRIPTION FACTOR DP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08781">
        <property type="entry name" value="DP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02319">
        <property type="entry name" value="WHD_E2F_TDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01138">
        <property type="entry name" value="DP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01372">
        <property type="entry name" value="E2F_TDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144074">
        <property type="entry name" value="E2F-DP heterodimerization region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="E2F/DP family winged-helix DNA-binding" evidence="10">
        <location>
            <begin position="233"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription factor DP C-terminal" evidence="9">
        <location>
            <begin position="319"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="504"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="504"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="524"/>
            <end position="534"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000003677.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="534" mass="58583" checksum="994F4593D0F59F96" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>F6WJS0</accession>
    <name>F6WJS0_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Peroxisomal trans-2-enoyl-CoA reductase</fullName>
            <ecNumber evidence="13">1.3.1.38</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Participates in chain elongation of fatty acids. Catalyzes the reduction of trans-2-enoyl-CoAs of varying chain lengths from 6:1 to 16:1, having maximum activity with 10:1 CoA. Has no 2,4-dienoyl-CoA reductase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>(2E)-decenoyl-CoA + NADPH + H(+) = decanoyl-CoA + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:44960"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:61406"/>
            <dbReference type="ChEBI" id="CHEBI:61430"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:44961"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>(2E)-dodecenoyl-CoA + NADPH + H(+) = dodecanoyl-CoA + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:44964"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57330"/>
            <dbReference type="ChEBI" id="CHEBI:57375"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:44965"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>(2E)-hexenoyl-CoA + NADPH + H(+) = hexanoyl-CoA + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:44956"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:62077"/>
            <dbReference type="ChEBI" id="CHEBI:62620"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:44957"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>(2E)-octenoyl-CoA + NADPH + H(+) = octanoyl-CoA + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:44952"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57386"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:62242"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:44953"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>(2E)-tetradecenoyl-CoA + NADPH + H(+) = tetradecanoyl-CoA + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:44968"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57385"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:61405"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:44969"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>a (2E)-enoyl-CoA + NADPH + H(+) = a 2,3-saturated acyl-CoA + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:33763"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58856"/>
            <dbReference type="ChEBI" id="CHEBI:65111"/>
            <dbReference type="EC" id="1.3.1.38"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:33764"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Interacts with PEX5, probably required to target it into peroxisomes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="1.3.1.38" evidence="13"/>
    <dbReference type="AlphaFoldDB" id="F6WJS0"/>
    <dbReference type="FunCoup" id="F6WJS0">
        <property type="interactions" value="65"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000008223"/>
    <dbReference type="Ensembl" id="ENSCINT00000008223.3">
        <property type="protein sequence ID" value="ENSCINP00000008223.3"/>
        <property type="gene ID" value="ENSCING00000003996.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156882"/>
    <dbReference type="HOGENOM" id="CLU_010194_1_2_1"/>
    <dbReference type="InParanoid" id="F6WJS0"/>
    <dbReference type="OMA" id="DETKDIW"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019166">
        <property type="term" value="F:trans-2-enoyl-CoA reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033306">
        <property type="term" value="P:phytol metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:001861">
        <property type="entry name" value="Peroxisomal trans-2-enoyl-CoA reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052388">
        <property type="entry name" value="Peroxisomal_t2-enoyl-CoA_red"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24317">
        <property type="entry name" value="PEROXISOMAL TRANS-2-ENOYL-COA REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24317:SF7">
        <property type="entry name" value="PEROXISOMAL TRANS-2-ENOYL-COA REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00041063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00049251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00049386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00049559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000008223.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="209" mass="22994" checksum="D32677C8F349A40E" modified="2012-04-18" version="2">MQCNIRKPDQVKTLFKNTVKMFGKLDYLVNNGGGQFLSPAENISPKGWHAVIETNLTGTFYCCQTVFNEWMAENGGVIVNIVVDMWRGYPFLSHTGAARAAVDNLTKSLAIEWISKGVRINSVAPGTIFSETAVANYPPNTFDEAKKIQPSCRLGVPEEISGVVCFLLSPAASFITGETVKVDGAQSLYDQPIKVPPHNKLPTWKWSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>F6WWQ0</accession>
    <accession>A0A1W5BEI0</accession>
    <name>F6WWQ0_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Kelch-like protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100182487</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">wild type</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01002889">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018668811.1">
        <property type="nucleotide sequence ID" value="XM_018813266.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6WWQ0"/>
    <dbReference type="Ensembl" id="ENSCINT00000002505.3">
        <property type="protein sequence ID" value="ENSCINP00000002505.3"/>
        <property type="gene ID" value="ENSCING00000014115.2"/>
    </dbReference>
    <dbReference type="GeneID" id="100182487"/>
    <dbReference type="GeneTree" id="ENSGT00940000165174"/>
    <dbReference type="HOGENOM" id="CLU_004253_14_6_1"/>
    <dbReference type="InParanoid" id="F6WWQ0"/>
    <dbReference type="OMA" id="ECESTNY"/>
    <dbReference type="OrthoDB" id="10250130at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031463">
        <property type="term" value="C:Cul3-RING ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990756">
        <property type="term" value="F:ubiquitin-like ligase-substrate adaptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18186">
        <property type="entry name" value="BTB_POZ_ZBTB_KLHL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017096">
        <property type="entry name" value="BTB-kelch_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011043">
        <property type="entry name" value="Gal_Oxase/kelch_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412">
        <property type="entry name" value="KELCH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412:SF497">
        <property type="entry name" value="KELCH-LIKE PROTEIN 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037037">
        <property type="entry name" value="Kelch-like_protein_gigaxonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50965">
        <property type="entry name" value="Galactose oxidase, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BTB" evidence="3">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000002505.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="501" mass="56393" checksum="A4A493DD43BF6449" modified="2012-04-18" version="2">MVLSCFSEYFQKLFTTEMKEKYKDCVEVNGVDVKSMTNLVEFMYTGEISINMDNVCYLLAVSDYLQMQFKELCIQYLMDATSPQNCFTIQVLADRFNIPVLSENNKKFIILNYKQVVSNDQFKRQSKEVATLFLQETHNQVSPDLVYTAVMNWVKFDLASNEKYLNELTQFVNFHELSPQILGDAVSVEPLLRKSDDNVDKTLKVFSYHMKNITSPGKSLICLGGARTLNKVMKYDLRRNEWSELPDLPVGRQLASAVVVDNVLYFIAGYLPTAGGNVKPTNIVHRMDLNKNFLQWEKVASVIEERYAAGATVLNGSIFLFGGISINNCRLSSGECFVTSLNKWIKVANMKTEEYFIVLVTLEDRIYLLGGVGTPSVACYDPTLSTWGNVAPMQTARKNLAAVVLNKAIYALGGKGAHEQVLKSVEKFNADNNTWVYVADMNIGRSHHTACVAQNKIYVVGGVNFTNKAVKSVECYDPQTNKWSIVCETEVAFFNHSLVAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>F6WXL3</accession>
    <name>F6WXL3_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="F6WXL3"/>
    <dbReference type="STRING" id="7719.ENSCINP00000021363"/>
    <dbReference type="Ensembl" id="ENSCINT00000021363.3">
        <property type="protein sequence ID" value="ENSCINP00000021363.3"/>
        <property type="gene ID" value="ENSCING00000010816.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165174"/>
    <dbReference type="HOGENOM" id="CLU_004253_11_0_1"/>
    <dbReference type="InParanoid" id="F6WXL3"/>
    <dbReference type="OMA" id="THNRFLA"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031463">
        <property type="term" value="C:Cul3-RING ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990756">
        <property type="term" value="F:ubiquitin-like ligase-substrate adaptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632:SF17">
        <property type="entry name" value="KELCH-LIKE PROTEIN 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632">
        <property type="entry name" value="LD33804P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="1">
        <location>
            <begin position="45"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000021363.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="250" mass="28413" checksum="A213F2E6FD52683D" modified="2012-04-18" version="2">MAMAAAETSFSINGTCSVEEQTHNIDHSSDLLKFANESRQDGCFNDIIIRVGEKNIPANKMVLSCYSKYFNTMFNTEMEEKYKDVVEVHGVDGESVEKLVDFMYTGKININTNNVCDLLAVSDFLQMPSVRKLCIEYLLTTVSPQNCFTIQALADRFTIPKLTEKFNNFVVENYQQVVSDEHFKKLSKDDVTKLLQSTHGKVPSDLVYTAVINWVKFELASNESHLSEVIKFVDFHEMSPEMLEDVVSVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>F6WYI9</accession>
    <name>F6WYI9_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">COP9 signalosome complex subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100177051</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CSN2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="F6WYI9"/>
    <dbReference type="FunCoup" id="F6WYI9">
        <property type="interactions" value="471"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000017753"/>
    <dbReference type="Ensembl" id="ENSCINT00000017753.3">
        <property type="protein sequence ID" value="ENSCINP00000017753.3"/>
        <property type="gene ID" value="ENSCING00000008702.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063301"/>
    <dbReference type="HOGENOM" id="CLU_028981_0_0_1"/>
    <dbReference type="InParanoid" id="F6WYI9"/>
    <dbReference type="OMA" id="SEENWKD"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008180">
        <property type="term" value="C:COP9 signalosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000338">
        <property type="term" value="P:protein deneddylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.570:FF:000001">
        <property type="entry name" value="Putative cop9 signalosome complex subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050871">
        <property type="entry name" value="26S_Proteasome/COP9_Components"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058796">
        <property type="entry name" value="COPS2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10678">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 11/COP9 SIGNALOSOME COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25983">
        <property type="entry name" value="COPS2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00753">
        <property type="entry name" value="PAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0736">Signalosome</keyword>
    <feature type="domain" description="PCI" evidence="8">
        <location>
            <begin position="252"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000017753.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="442" mass="51223" checksum="804EE297411F7A4A" modified="2012-04-18" version="2">MDDGDFMCDSDVDYDLEYSEDDNSEPDVDLENQYYNSKALKEDDAALALESFQRVLDLEEQKGEWGFKALKQMSKINFKLGRYEEMMSRYKQLLTYIKSAVTRNYSEKSINSILDYISTSKQMDLLQHFYETTLEALKDAKNDRLWFKTNTKLGKLYFDRDEYNKLAKILKQLHQSCTTQDGGDDMKKGTQLLEVYALEIQMYTAQKNNKKLKHVYEQSLHIKSAIPHPFTMGVIRECGGKMHLREGEYDKAHTDFFEAFKNYDESGSPRRTTCLKYLVLANMLMKSGINPFDSQEAKPYKNDPDILAMTNLVSAYQNNDIAAFESIVAANRASIMQDPFIREHIEDLLRNVRTQVLCKLIRPYTRIRIPFISKELNIDTDEVESLLVSCILDGTIQGRIDQVEQVLVIDQHNAALSSRYHAIDRWTEQLSTLHLAVANRLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>F6XEY2</accession>
    <name>F6XEY2_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01001823">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6XEY2"/>
    <dbReference type="Ensembl" id="ENSCINT00000023705.2">
        <property type="protein sequence ID" value="ENSCINP00000023459.2"/>
        <property type="gene ID" value="ENSCING00000012608.2"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1997607_0_0_1"/>
    <dbReference type="InParanoid" id="F6XEY2"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000023459.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="125" mass="13794" checksum="2C1C148C49ED63B3" modified="2012-04-18" version="2">MGERVFSTAVDTNSVQAPPRDGLQALSPISYTLIYSCVAVVLFLFVLFTLWVIAQCCDRNRPFVTRKRLVNSHHFLRRNQSMCEPLYGSTTCISTYVEDGIGSVVNASNSGKATVPVRYISNSAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>F6XLA1</accession>
    <name>F6XLA1_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Zinc finger protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
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    <reference evidence="18" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
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    <reference evidence="23" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">wild type</strain>
        </source>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">mRNA decapping enzyme that specifically removes the nicotinamide adenine dinucleotide (NAD) cap from a subset of mRNAs by hydrolyzing the diphosphate linkage to produce nicotinamide mononucleotide (NMN) and 5' monophosphate mRNA. The NAD-cap is present at the 5'-end of some RNAs; in contrast to the canonical N7 methylguanosine (m7G) cap, the NAD cap promotes mRNA decay. Preferentially acts on NAD-capped transcripts in response to nutrient stress. Also acts on free nicotinamide adenine dinucleotide molecules: hydrolyzes NAD(H) into NMN(H) and AMP, and NADPH into NMNH and 2',5'-ADP. May act to regulate the concentration of peroxisomal nicotinamide nucleotide cofactors required for oxidative metabolism in this organelle. Regulates the levels of circadian clock components PER1, PER2, PER3 and CRY2 in the liver.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>NAD(+) + H2O = beta-nicotinamide D-ribonucleotide + AMP + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:11800"/>
            <dbReference type="ChEBI" id="CHEBI:14649"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="3.6.1.22"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:11801"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>NADH + H2O = reduced beta-nicotinamide D-ribonucleotide + AMP + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:48868"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:90832"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="3.6.1.22"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:48869"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>NADPH + H2O = reduced beta-nicotinamide D-ribonucleotide + adenosine 2',5'-bisphosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:60820"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:90832"/>
            <dbReference type="ChEBI" id="CHEBI:194156"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:60821"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a 5'-end NAD(+)-phospho-ribonucleoside in mRNA + H2O = a 5'-end phospho-adenosine-phospho-ribonucleoside in mRNA + beta-nicotinamide D-ribonucleotide + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:60876"/>
            <dbReference type="Rhea" id="RHEA-COMP:15698"/>
            <dbReference type="Rhea" id="RHEA-COMP:15719"/>
            <dbReference type="ChEBI" id="CHEBI:14649"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:144029"/>
            <dbReference type="ChEBI" id="CHEBI:144051"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:60877"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="16">Homodimer. Homodimerization is essential for its catalytic activity and protein stability. Interacts (via ANK repeats) with BLMH.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasmic granule</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the Nudix hydrolase family. NudC subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.22" evidence="5"/>
    <dbReference type="EMBL" id="EAAA01002093">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6XNV2"/>
    <dbReference type="FunCoup" id="F6XNV2">
        <property type="interactions" value="5"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000012890"/>
    <dbReference type="Ensembl" id="ENSCINT00000012890.3">
        <property type="protein sequence ID" value="ENSCINP00000012890.3"/>
        <property type="gene ID" value="ENSCING00000006248.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157592"/>
    <dbReference type="HOGENOM" id="CLU_037162_0_2_1"/>
    <dbReference type="InParanoid" id="F6XNV2"/>
    <dbReference type="OMA" id="CNTRTTL"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035529">
        <property type="term" value="F:NADH pyrophosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019677">
        <property type="term" value="P:NAD+ catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006742">
        <property type="term" value="P:NADP+ catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03429">
        <property type="entry name" value="NUDIX_NADH_pyrophosphatase_Nudt13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.79.10:FF:000023">
        <property type="entry name" value="Peroxisomal NADH pyrophosphatase NUDT12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050241">
        <property type="entry name" value="NAD-cap_RNA_hydrolase_NudC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015375">
        <property type="entry name" value="NADH_PPase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049734">
        <property type="entry name" value="NudC-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001299">
        <property type="entry name" value="PRK00241.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42904:SF6">
        <property type="entry name" value="NAD-CAPPED RNA HYDROLASE NUDT12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42904">
        <property type="entry name" value="NUDIX HYDROLASE, NUDC SUBFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09296">
        <property type="entry name" value="NUDIX-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="20" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0520">NAD</keyword>
    <keyword evidence="9" id="KW-0521">NADP</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ANK" evidence="20">
        <location>
            <begin position="45"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="Nudix hydrolase" evidence="21">
        <location>
            <begin position="307"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00030313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00031178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00045837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00046702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00049196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00049264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000012890.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="454" mass="50941" checksum="FA2E358B053F143C" modified="2012-04-18" version="2">KMSGNSNAQKYYIDEIFTAAANGDVEAITKGYNMLSISPDVTDKMGWTPLMCAARNGHVNVMGFLLQQGADPKHMNKSGQTALDVASFWNQTGSVNFLNGFLKPNKSPVMEVVHYFGFSALDRQSYQRSDENEMKKAMRMENAKYVVFADLQLLVSNDAVTGKSQNVVYLTWGEIEKYLSIVDHDLIFLGVGNIDKGLLVRETHHAQLNDSPYFAVNFKKVSRDDEFSPEKYNGSFTDKDNRRLMMMLQKNESGLVAQARSVLAWHERYNFCPTCGNETIMKDFGYKRLCISESCTTHKGAHNTSFPRTDPVVIILVASKDGSKCLLGRQSRFPRGMYSCIAGFMEPGESIEDAARREVFEESGVKVGQVEYHSSQPWPFPSNIMIGLIGRAVCDDINVDKVELEDARWFDKPEVAKAILEGFGRKEGLVVPPHTAIAHHLIKTWVQRNSNSNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>F6YAK0</accession>
    <name>F6YAK0_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01001256">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6YAK0"/>
    <dbReference type="Ensembl" id="ENSCINT00000026397.2">
        <property type="protein sequence ID" value="ENSCINP00000026151.2"/>
        <property type="gene ID" value="ENSCING00000014462.2"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_796820_0_0_1"/>
    <dbReference type="InParanoid" id="F6YAK0"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="214"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="219"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000026151.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="348" mass="38188" checksum="38C538374D3860D6" modified="2012-04-18" version="2">MLPVRKHPAKRDGYTLHLLQNLKLEKSKSLEKDSAQSPAETAPPELYAIIPDPVPQEDETQTPEPSPTIDLPQLLPRIAQKPTQQPRPVRSPRIKAKPRPTAPPPPRPPGPPPQRPITADVSPTNKPEKPRKPSTDSVKTPPARPPPPSAQKIFASKIRPKRSDSELHISPVKLPSPLKTKVTSSPIIQYPEQPKIISDQAFADVDDVTYSAIPDWSGDSFSTDTSEDNSFTESVGGAKETPKKKKLSLGTLASSVSTLRMKLGRHKSRDVIAEASPYSTASDVEPKGFEGVAEGVYAGIDDYSVNYNENWNSNETSDYKGDVYLAATENRSFVYAELDHTNFVDDGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>F6YDS2</accession>
    <name>F6YDS2_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Histone-lysine N-methyltransferase</fullName>
            <ecNumber evidence="12">2.1.1.355</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-lysyl(9)-[histone H3] + 3 S-adenosyl-L-methionine = N(6),N(6),N(6)-trimethyl-L-lysyl(9)-[histone H3] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:60276"/>
            <dbReference type="Rhea" id="RHEA-COMP:15538"/>
            <dbReference type="Rhea" id="RHEA-COMP:15546"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="EC" id="2.1.1.355"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Centromere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. Suvar3-9 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.355" evidence="12"/>
    <dbReference type="AlphaFoldDB" id="F6YDS2"/>
    <dbReference type="FunCoup" id="F6YDS2">
        <property type="interactions" value="38"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000024599"/>
    <dbReference type="Ensembl" id="ENSCINT00000024845.2">
        <property type="protein sequence ID" value="ENSCINP00000024599.2"/>
        <property type="gene ID" value="ENSCING00000000736.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156788"/>
    <dbReference type="HOGENOM" id="CLU_020840_8_0_1"/>
    <dbReference type="InParanoid" id="F6YDS2"/>
    <dbReference type="OMA" id="YESNEFT"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000775">
        <property type="term" value="C:chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046974">
        <property type="term" value="F:histone H3K9 methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140949">
        <property type="term" value="F:histone H3K9 trimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00024">
        <property type="entry name" value="CD_CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10542">
        <property type="entry name" value="SET_SUV39H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023779">
        <property type="entry name" value="Chromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011381">
        <property type="entry name" value="H3-K9_MeTrfase_SUV39H1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050973">
        <property type="entry name" value="H3K9_Histone-Lys_N-MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003616">
        <property type="entry name" value="Post-SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007728">
        <property type="entry name" value="Pre-SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46223">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE SUV39H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46223:SF4">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05033">
        <property type="entry name" value="Pre-SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009343">
        <property type="entry name" value="SUV39_SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00468">
        <property type="entry name" value="PreSET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00598">
        <property type="entry name" value="CHROMO_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50868">
        <property type="entry name" value="POST_SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50867">
        <property type="entry name" value="PRE_SET"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0137">Centromere</keyword>
    <keyword evidence="9 12" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7 12 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 12" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 12 13" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Chromo" evidence="14">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="domain" description="Pre-SET" evidence="16">
        <location>
            <begin position="197"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="SET" evidence="15">
        <location>
            <begin position="262"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="Post-SET" evidence="17">
        <location>
            <begin position="418"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
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        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
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        <location>
            <position position="201"/>
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        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>4</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR009343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR009343-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000024599.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="434" mass="50310" checksum="A5143959424DA510" modified="2012-04-18" version="2">YYLVKWVGWPMEANTWEPADNLSCPNLIHDFHEDYTKQLNKFLKERKLSWKTTRTLFRNLDKYIKFTGKNELVTPEAMVSEIQPWLKYNWFKDLLTLKVNLALFPFFYSCSCNRKKRKVFSKNFLNRHRQILIYAEEKRKVLHQLQMWQKRINEISKSMPLIAVENEVDLCSFPPEFEYVESNITGKDVIIPTDPLIGCDCTNGCTSRLISNGCCPGIHKGRAPYANKLVKIKPGKAIFECNRRCKCGVDCPNRVVQHGPRNALSIYRTSNGKGWGVKTLQFIPKGTFVMEYVGEVITNDEAERRGKQYDNNGITYLFDLDYYDSENPLTVDATRYGNISHFVNHSCSPNLQVYNVFINNLDPSLPRIALFAKCNIGTNEELTFDYQMTGDNTTVYDKTIRNRYLAYATDTTNPSSIKRTRCLCASPNCREWLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>F6YIU9</accession>
    <name>F6YIU9_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Integrator complex subunit 14</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">von Willebrand factor A domain-containing protein 9</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">wild type</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="6">Component of the Integrator complex, composed of core subunits INTS1, INTS2, INTS3, INTS4, INTS5, INTS6, INTS7, INTS8, INTS9/RC74, INTS10, INTS11/CPSF3L, INTS12, INTS13, INTS14 and INTS15. The core complex associates with protein phosphatase 2A subunits PPP2CA and PPP2R1A, to form the Integrator-PP2A (INTAC) complex. INTS14 is part of the tail subcomplex, composed of INTS10, INTS13, INTS14 and INTS15.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the Integrator subunit 14 family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002714">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6YIU9"/>
    <dbReference type="FunCoup" id="F6YIU9">
        <property type="interactions" value="759"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000015311"/>
    <dbReference type="Ensembl" id="ENSCINT00000015311.3">
        <property type="protein sequence ID" value="ENSCINP00000015311.3"/>
        <property type="gene ID" value="ENSCING00000007459.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000009486"/>
    <dbReference type="HOGENOM" id="CLU_041485_0_0_1"/>
    <dbReference type="InParanoid" id="F6YIU9"/>
    <dbReference type="OMA" id="QSSVVWI"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032039">
        <property type="term" value="C:integrator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034472">
        <property type="term" value="P:snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039841">
        <property type="entry name" value="INTS14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045814">
        <property type="entry name" value="IntS14_b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046471">
        <property type="entry name" value="IntS14_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13532">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13532:SF3">
        <property type="entry name" value="INTEGRATOR COMPLEX SUBUNIT 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19435">
        <property type="entry name" value="IntS14_b-barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20504">
        <property type="entry name" value="IntS14_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13519">
        <property type="entry name" value="VWA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VWFA" evidence="7">
        <location>
            <begin position="3"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="Integrator complex subunit 14 beta-barrel" evidence="8">
        <location>
            <begin position="208"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="Integrator complex subunit 14 C-terminal" evidence="9">
        <location>
            <begin position="385"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00029992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00061449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00065091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF19435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF20504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000015311.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="486" mass="54031" checksum="DA50A8E41FA783BB" modified="2012-04-18" version="2">MPVIVAVDASLSMWRNVFEGEEKKTTRFNIAEECTNQILDHVTSKFKLESMALLSCSSSVTVLSDFTRDLSMLKNGLKALKIGDKADFSSFVNYLNHFVVKTWGRMQSCTVIVITDGGIVSVESLDALKNAQTKNSLFEFPCNLHFLCIETLQNLKLSGSYYLLHEILKINEDLGSVVPLLETKTPEMGLPGMVATSIDKFLNNTLKTFETTLSCGNLSSRISIFPSPKAQKSINSSLEAEIFTVKDKISIVGFLNISDIRNPPVLSRHLLLPITPTEVEDISTHPSFSVVLHGSLKMESKVALVKLATEWFGIIYSWADSKTKSNLVLSILPVGLNCIPWLGNFNQLAPASMWPESLSSKKPSIPSFPVQSKRFMKRSYSQPTVAWTRATGLQTDVQKILRSAKKLPEKSHVFYKDLNRLRRAALSFGFHDLLDGISALLERECTLLPGTAHPEATLQLSHAARELKSTINKGHEHTIEPLKTDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>F6YKS6</accession>
    <name>F6YKS6_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RZ-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="10">wild type</strain>
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    <reference evidence="10" key="3">
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        <scope>IDENTIFICATION</scope>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="ENSCING00000007808.3"/>
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        <property type="component" value="Chromosome 10"/>
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        <property type="entry name" value="SF1_C_Upf1"/>
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        <property type="entry name" value="DNA2/NAM7-like"/>
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        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
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        <property type="entry name" value="P-loop_NTPase"/>
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        <property type="entry name" value="SF1_C_Upf1"/>
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    <dbReference type="InterPro" id="IPR046439">
        <property type="entry name" value="ZF_RZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000967">
        <property type="entry name" value="Znf_NFX1"/>
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    <dbReference type="InterPro" id="IPR057373">
        <property type="entry name" value="ZNFX1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887">
        <property type="entry name" value="DNA2/NAM7 HELICASE FAMILY"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887:SF341">
        <property type="entry name" value="NFX1-TYPE ZINC FINGER-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20173">
        <property type="entry name" value="ZnF_RZ-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25396">
        <property type="entry name" value="ZNFX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00438">
        <property type="entry name" value="ZnF_NFX"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS51981">
        <property type="entry name" value="ZF_RZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0391">Immunity</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RZ-type" evidence="9">
        <location>
            <begin position="1681"/>
            <end position="1758"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="134"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="801"/>
            <end position="828"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000003056.3"/>
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    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="1773" mass="203270" checksum="D772A21763588C4C" modified="2025-10-08" version="3"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>F6YMG8</accession>
    <name>F6YMG8_CIOIN</name>
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        <recommendedName>
            <fullName evidence="13">Sodium channel protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">wild type</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Cell membrane</location>
            <topology evidence="1 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the sodium channel (TC 1.A.1.10) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002197">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000016580"/>
    <dbReference type="Ensembl" id="ENSCINT00000016580.3">
        <property type="protein sequence ID" value="ENSCINP00000016580.3"/>
        <property type="gene ID" value="ENSCING00000008113.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000167926"/>
    <dbReference type="HOGENOM" id="CLU_486239_0_0_1"/>
    <dbReference type="InParanoid" id="F6YMG8"/>
    <dbReference type="OMA" id="DITICDN"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001518">
        <property type="term" value="C:voltage-gated sodium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005248">
        <property type="term" value="F:voltage-gated sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000093">
        <property type="entry name" value="Calcium channel subunit Cch1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001696">
        <property type="entry name" value="Na_channel_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043203">
        <property type="entry name" value="VGCC_Ca_Na"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10037:SF62">
        <property type="entry name" value="SODIUM CHANNEL PROTEIN 60E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10037">
        <property type="entry name" value="VOLTAGE-GATED CATION CHANNEL CALCIUM AND SODIUM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00170">
        <property type="entry name" value="NACHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8 13" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="13" id="KW-0915">Sodium</keyword>
    <keyword evidence="13" id="KW-0894">Sodium channel</keyword>
    <keyword evidence="13" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 13" id="KW-0813">Transport</keyword>
    <keyword evidence="13" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="58"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="106"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="203"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="15">
        <location>
            <begin position="2"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="535"/>
            <end position="561"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
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                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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            <dbReference type="PubMed" id="18854010"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">wild type</strain>
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                <person name="Levine M."/>
                <person name="Satoh N."/>
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            </authorList>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="F6Z1P6"/>
    <dbReference type="Ensembl" id="ENSCINT00000027833.2">
        <property type="protein sequence ID" value="ENSCINP00000027587.2"/>
        <property type="gene ID" value="ENSCING00000015647.2"/>
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    <dbReference type="GeneTree" id="ENSGT00530000067254"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="ECM_Regulatory_Proteins"/>
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        <property type="entry name" value="FN3_dom"/>
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        <property type="entry name" value="FN3_sf"/>
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    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46708:SF2">
        <property type="entry name" value="FIBRONECTIN TYPE-III DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46708">
        <property type="entry name" value="TENASCIN"/>
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    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Fibronectin type-III" evidence="3">
        <location>
            <begin position="68"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="3">
        <location>
            <begin position="173"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="240"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000027587.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="261" mass="29117" checksum="44545519AF468049" modified="2012-04-18" version="2">MLHKLQNEQRILVDQTIQARRFATYTNLESGTRYFVTVYPSTISGIIIPGAVGRASTTTLESTELVSRNTTISTTITSVGPHHVTLSWQRRAGVANSLIFFLRMGGMPAILQRKLVSRYHSSITFQSLRPDTNYLITAHSLLNTTLLLRASVTTSRRIPSLEEAITDNYDRSLVPPTGLVVLSSTTESVLLQWNLVAPGLQYNVRLYKAGTNVLSVFRQKIRQTQITMSQLRPGRYEATVSSYDPLSKEESSESPRVSVNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>F6Z6H7</accession>
    <accession>A0A1W2WIL3</accession>
    <name>F6Z6H7_CIOIN</name>
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        <recommendedName>
            <fullName evidence="10">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="10">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC100179894</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">wild type</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the helicase family. RecQ subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="10"/>
    <dbReference type="EMBL" id="EAAA01001228">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="EAAA01001229">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002131753.1">
        <property type="nucleotide sequence ID" value="XM_002131717.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6Z6H7"/>
    <dbReference type="FunCoup" id="F6Z6H7">
        <property type="interactions" value="78"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000004562"/>
    <dbReference type="Ensembl" id="ENSCINT00000004562.3">
        <property type="protein sequence ID" value="ENSCINP00000004562.3"/>
        <property type="gene ID" value="ENSCING00000002232.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100179894"/>
    <dbReference type="KEGG" id="cin:100179894"/>
    <dbReference type="GeneTree" id="ENSGT00940000159168"/>
    <dbReference type="HOGENOM" id="CLU_001103_14_3_1"/>
    <dbReference type="InParanoid" id="F6Z6H7"/>
    <dbReference type="OMA" id="VGLTHEI"/>
    <dbReference type="OrthoDB" id="10261556at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="GO_Central"/>
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        <property type="term" value="P:double-strand break repair via homologous recombination"/>
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        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ATP-dependent DNA helicase"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="ATP-dependent DNA helicase"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Werner syndrome RecQ like helicase"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="HRDC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004589">
        <property type="entry name" value="DNA_helicase_ATP-dep_RecQ"/>
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    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029491">
        <property type="entry name" value="Helicase_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010997">
        <property type="entry name" value="HRDC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002121">
        <property type="entry name" value="HRDC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044876">
        <property type="entry name" value="HRDC_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032284">
        <property type="entry name" value="RecQ_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018982">
        <property type="entry name" value="RQC_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00614">
        <property type="entry name" value="recQ_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710:SF120">
        <property type="entry name" value="BIFUNCTIONAL 3'-5' EXONUCLEASE_ATP-DEPENDENT HELICASE WRN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710">
        <property type="entry name" value="DNA HELICASE RECQ FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00570">
        <property type="entry name" value="HRDC"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="HTH_40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16124">
        <property type="entry name" value="RecQ_Zn_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RQC"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HRDC"/>
        <property type="match status" value="1"/>
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    <dbReference type="SMART" id="SM00956">
        <property type="entry name" value="RQC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47819">
        <property type="entry name" value="HRDC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50967">
        <property type="entry name" value="HRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="13">
        <location>
            <begin position="267"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="14">
        <location>
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                <person name="Ueno K."/>
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                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
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                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
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                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_026694503.1">
        <property type="nucleotide sequence ID" value="XM_026838702.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_026694504.1">
        <property type="nucleotide sequence ID" value="XM_026838703.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6ZSX6"/>
    <dbReference type="STRING" id="7719.ENSCINP00000007879"/>
    <dbReference type="Ensembl" id="ENSCINT00000007879.3">
        <property type="protein sequence ID" value="ENSCINP00000007879.3"/>
        <property type="gene ID" value="ENSCING00000003822.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100178499"/>
    <dbReference type="KEGG" id="cin:100178499"/>
    <dbReference type="GeneTree" id="ENSGT00940000164257"/>
    <dbReference type="HOGENOM" id="CLU_615307_0_0_1"/>
    <dbReference type="InParanoid" id="F6ZSX6"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051879">
        <property type="term" value="F:Hsp90 protein binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22904:SF523">
        <property type="entry name" value="STRESS-INDUCED-PHOSPHOPROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22904">
        <property type="entry name" value="TPR REPEAT CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0802">TPR repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="376"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="386"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="403"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="424"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="436"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000007879.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="445" mass="50015" checksum="022E2DA207C83A13" modified="2012-04-18" version="2">MLPPVPSLIESSGEEDNLFSDDEKYPDKVFGEFPWGTNRSTNAGTQVEMWGKLSRTYQHEVLDCCHVLLVAPILFTRQTMPLKYMNWASECGLSSLERLMADVSEEGNVLDSHELVGLDMLREAHHALRSVVTSRVDNVAVVKFAKEIDQLKINDIKRAIALGSTQPQIKRLIDAMSCVADEHPPSADPPPKLKMNLYDFASSDDRKVVAALMTARQYAEVLHMMGQEGRDVVKELLAQQTEKDEMIGYKESGNKYFKESKFSEAVKQYSIAIKSYPYIAVLYSNRCKAAINLHNYKLAISDAYRAVTLKPEWGKAYYRLADALNHDGQYAAAIRKIDMGLTKCFNITERQELSKIRLALEEKLVKAGISHLALNGINNNLPEKPKKTKKKKKKSKAQATEETESKAGQNKDLETKSETKENKNPNSDVISGNNDVIKKHEECGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>F6ZYP8</accession>
    <name>F6ZYP8_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">PAZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">wild type</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the argonaute family. Piwi subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01000697">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6ZYP8"/>
    <dbReference type="STRING" id="7719.ENSCINP00000029631"/>
    <dbReference type="Ensembl" id="ENSCINT00000029877.2">
        <property type="protein sequence ID" value="ENSCINP00000029631.2"/>
        <property type="gene ID" value="ENSCING00000017557.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183200"/>
    <dbReference type="HOGENOM" id="CLU_008813_3_0_1"/>
    <dbReference type="InParanoid" id="F6ZYP8"/>
    <dbReference type="OMA" id="CHNIRED"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031047">
        <property type="term" value="P:regulatory ncRNA-mediated gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02845">
        <property type="entry name" value="PAZ_piwi_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.260.10:FF:000003">
        <property type="entry name" value="Piwi-like RNA-mediated gene silencing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.260.10">
        <property type="entry name" value="paz domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003100">
        <property type="entry name" value="PAZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036085">
        <property type="entry name" value="PAZ_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003165">
        <property type="entry name" value="Piwi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22891">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02170">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00949">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101690">
        <property type="entry name" value="PAZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50821">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50822">
        <property type="entry name" value="PIWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0943">RNA-mediated gene silencing</keyword>
    <keyword evidence="4" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="PAZ" evidence="8">
        <location>
            <begin position="56"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="Piwi" evidence="9">
        <location>
            <begin position="333"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000029631.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="374" mass="44059" checksum="A8D36FE7E3B452DE" modified="2012-04-18" version="2">MKQVGRNFYNPSMSFTVECQRVNFELWPGFITSILQYEKSVLLCAEVSHKLMRKDSVLDILRQMYGDCRQRGRDFKQEMEKFLVGQIVLTRYNNKTYRIDGIEWNLNVNMKFERKSGSVSYVDYYKEQYNIVIRDTNQPLLLSRPKQSEIRKGGLEVVHLVPELCTVTGLTDELRADFNTMKRLAVYTKQGPTKRKQALKSFIQRITTNTEVEKRFAEWGLRFEDRLLDLKGRVLDSETIMFGDNKQAQSRDASWDQEFRRQRLLKCIDLQEWAILFCSRDKRCAEDFVEKLLKVSRNMGFRVARPTPVELESDQMFQKRIRDVMGRNPKTQLICCMMPSSRKDRYEGIKKVCCVDMPVPSQVVLSRTLSKPQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F6ZZN5</accession>
    <name>F6ZZN5_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="F6ZZN5"/>
    <dbReference type="Ensembl" id="ENSCINT00000000523.3">
        <property type="protein sequence ID" value="ENSCINP00000000523.3"/>
        <property type="gene ID" value="ENSCING00000016113.2"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3013475_0_0_1"/>
    <dbReference type="InParanoid" id="F6ZZN5"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000000523.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="56" mass="5998" checksum="96F8D1AC91BFA4C6" modified="2012-04-18" version="2">MPLVGGDNATTMTSQSTDPLLNMLLRLQEAATNYESDASHDDAFELSLDRALGDLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>F7A1G9</accession>
    <name>F7A1G9_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Metalloendopeptidase</fullName>
            <ecNumber evidence="11">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">wild type</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="10 11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="10 11">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="11"/>
    <dbReference type="EMBL" id="EAAA01002761">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F7A1G9"/>
    <dbReference type="Ensembl" id="ENSCINT00000017081.3">
        <property type="protein sequence ID" value="ENSCINP00000017081.3"/>
        <property type="gene ID" value="ENSCING00000008374.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183111"/>
    <dbReference type="HOGENOM" id="CLU_021966_0_0_1"/>
    <dbReference type="InParanoid" id="F7A1G9"/>
    <dbReference type="OMA" id="RMYDCAE"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06263">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04280">
        <property type="entry name" value="ZnMc_astacin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000015">
        <property type="entry name" value="Meprin A subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.210.10">
        <property type="entry name" value="Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034035">
        <property type="entry name" value="Astacin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000998">
        <property type="entry name" value="MAM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002083">
        <property type="entry name" value="MATH/TRAF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001506">
        <property type="entry name" value="Peptidase_M12A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006026">
        <property type="entry name" value="Peptidase_Metallo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008974">
        <property type="entry name" value="TRAF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10127">
        <property type="entry name" value="DISCOIDIN, CUB, EGF, LAMININ , AND ZINC METALLOPROTEASE DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10127:SF856">
        <property type="entry name" value="METALLOENDOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01400">
        <property type="entry name" value="Astacin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00629">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF22486">
        <property type="entry name" value="MATH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00480">
        <property type="entry name" value="ASTACIN"/>
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        <property type="entry name" value="MAMDOMAIN"/>
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        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00235">
        <property type="entry name" value="ZnMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
        <property type="entry name" value="TRAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51864">
        <property type="entry name" value="ASTACIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50060">
        <property type="entry name" value="MAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50144">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 10 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 10 11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1 10 11" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="5 10 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Peptidase M12A" evidence="14">
        <location>
            <begin position="28"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="MAM" evidence="12">
        <location>
            <begin position="229"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="MATH" evidence="13">
        <location>
            <begin position="397"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="119"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51864"/>
        </source>
    </evidence>
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                <person name="Hastings K.E."/>
                <person name="Ho I."/>
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                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
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                <person name="Yoshizaki F."/>
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                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
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                <person name="Detter C."/>
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                <person name="Richardson P."/>
                <person name="Lucas S."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Yamada L."/>
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                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">wild type</strain>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Hastings K.E."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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        </source>
    </evidence>
    <sequence length="183" mass="19593" checksum="7C701C467EE74011" modified="2012-04-18" version="2">MNFIHLKLAGTPYTSIVSDAARTETCWWNSLEPAASFDKHEMCDSSTNCRYGGICWSSRSDVSVPQGHRRSHRGTSPHVVCRCTMHCQSLSGGVGTHPDSLVCGSDGRTYNNECQLRLAACRCQFPITVFKVGACERGSSPHMTPSARPHDLSTDAGSGSGSDVIDICSGGVTCRFGAVCDVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>F7AXN3</accession>
    <name>F7AXN3_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ATP-dependent RNA helicase</fullName>
            <ecNumber evidence="8">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">wild type</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">RNA helicase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="domain">
        <text evidence="8">The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DEAD box helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="8"/>
    <dbReference type="EMBL" id="EAAA01001979">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F7AXN3"/>
    <dbReference type="FunCoup" id="F7AXN3">
        <property type="interactions" value="421"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000007520"/>
    <dbReference type="Ensembl" id="ENSCINT00000007520.3">
        <property type="protein sequence ID" value="ENSCINP00000007520.3"/>
        <property type="gene ID" value="ENSCING00000003655.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00550000074847"/>
    <dbReference type="HOGENOM" id="CLU_003041_13_1_1"/>
    <dbReference type="InParanoid" id="F7AXN3"/>
    <dbReference type="OMA" id="QMIQKAR"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd17946">
        <property type="entry name" value="DEADc_DDX24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24031">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7" id="KW-0347">Helicase</keyword>
    <keyword evidence="2 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="12">
        <location>
            <begin position="163"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="195"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="11">
        <location>
            <begin position="460"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="115"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="6">
        <location>
            <begin position="163"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="120"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU365068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000007520.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="691" mass="78759" checksum="3150702D0BD1C2E8" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>F7BJP4</accession>
    <name>F7BJP4_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Carbohydrate sulfotransferase</fullName>
            <ecNumber evidence="9">2.8.2.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">wild type</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Golgi apparatus membrane</location>
            <topology evidence="1 9">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the sulfotransferase 2 family.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="9"/>
    <dbReference type="EMBL" id="EAAA01000833">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F7BJP4"/>
    <dbReference type="Ensembl" id="ENSCINT00000021187.3">
        <property type="protein sequence ID" value="ENSCINP00000021187.3"/>
        <property type="gene ID" value="ENSCING00000016854.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164429"/>
    <dbReference type="HOGENOM" id="CLU_043398_1_2_1"/>
    <dbReference type="InParanoid" id="F7BJP4"/>
    <dbReference type="OMA" id="RCESSHY"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016051">
        <property type="term" value="P:carbohydrate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018011">
        <property type="entry name" value="Carb_sulfotrans_8-10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005331">
        <property type="entry name" value="Sulfotransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12137">
        <property type="entry name" value="CARBOHYDRATE SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12137:SF54">
        <property type="entry name" value="CARBOHYDRATE SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03567">
        <property type="entry name" value="Sulfotransfer_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="8 9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000021187.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="281" mass="33472" checksum="A8E9C24BD804FF03" modified="2012-04-18" version="2">MDSDTKTSQPQEPHITRLKEISSRCESSHYINKRIDLSRFVFDDEHKLIMCVVPKAACTTWKRIMWYLNGCENDKEKYIMTFNFSHIQVLIFSSVSKESAIEKLRSYTKFFVKRSPFERLVSAYRNKFITSKNPNYREKIGKQYAKERLRKYLVTIRHGNLTFEQFTSHIVKATEYNLPGELDVHWRPQVELCNPCALKYDYVIDFRKMATESNELLQYVQRNDDVMDQIRLKETHRVLTNDDTVASHMNLIDDNVKLKLKRLYENDSYILGYSPMRLKNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>F7BMQ6</accession>
    <accession>A0A1W2WEJ4</accession>
    <name>F7BMQ6_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Centrosomal protein CCDC61</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Coiled-coil domain-containing protein 61</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">VFL3 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100175600</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">wild type</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium basal body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the CCDC61 family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001765">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002127205.1">
        <property type="nucleotide sequence ID" value="XM_002127169.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F7BMQ6"/>
    <dbReference type="FunCoup" id="F7BMQ6">
        <property type="interactions" value="11"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000008976"/>
    <dbReference type="Ensembl" id="ENSCINT00000008976.3">
        <property type="protein sequence ID" value="ENSCINP00000008976.3"/>
        <property type="gene ID" value="ENSCING00000004344.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100175600"/>
    <dbReference type="KEGG" id="cin:100175600"/>
    <dbReference type="GeneTree" id="ENSGT00940000154133"/>
    <dbReference type="HOGENOM" id="CLU_038746_1_0_1"/>
    <dbReference type="InParanoid" id="F7BMQ6"/>
    <dbReference type="OMA" id="GREDWRI"/>
    <dbReference type="OrthoDB" id="568137at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd22284">
        <property type="entry name" value="HD_CCDC61_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049733">
        <property type="entry name" value="CCDC61_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22691:SF1">
        <property type="entry name" value="CENTROSOMAL PROTEIN CCDC61"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22691">
        <property type="entry name" value="YEAST SPT2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0966">Cell projection</keyword>
    <keyword evidence="3 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="286"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="150"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="288"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="349"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="361"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="385"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="437"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="482"/>
            <end position="495"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00041518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00042326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000008976.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="516" mass="60123" checksum="8EE3652E83C934BE" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>F7BPM3</accession>
    <name>F7BPM3_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">UBX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lipid droplet</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="F7BPM3"/>
    <dbReference type="FunCoup" id="F7BPM3">
        <property type="interactions" value="938"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000008621"/>
    <dbReference type="Ensembl" id="ENSCINT00000008621.3">
        <property type="protein sequence ID" value="ENSCINP00000008621.3"/>
        <property type="gene ID" value="ENSCING00000004167.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000168542"/>
    <dbReference type="HOGENOM" id="CLU_047924_0_1_1"/>
    <dbReference type="InParanoid" id="F7BPM3"/>
    <dbReference type="OMA" id="QISVRCP"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049483">
        <property type="entry name" value="FAF1_2-like_UAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006577">
        <property type="entry name" value="UAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001012">
        <property type="entry name" value="UBX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050730">
        <property type="entry name" value="UBX_domain-protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23322:SF1">
        <property type="entry name" value="FAS-ASSOCIATED FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23322">
        <property type="entry name" value="FAS-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21021">
        <property type="entry name" value="FAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00789">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00594">
        <property type="entry name" value="UAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50033">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0551">Lipid droplet</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBX" evidence="5">
        <location>
            <begin position="243"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="196"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="201"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000008621.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="342" mass="39403" checksum="3BC7E97EF158C469" modified="2012-04-18" version="2">FIRFVWRIFNPDPREMVTDPRGDVLTFVDEFEAKYENPPQMFRGSYGEAQAEAKKNLQFLLVYLHDPHNKDSEPFCSGTLCNNDVVEYINTNMLFWGCSIQKPEGYKVSKLIRNPTYPLVAVVCLYQNQLAIVGRVQGKVTAENSVNLLREIVDLYEPVLVSARADREALNQNQQIRAEQDEAYLLSLEKDKQKMDKKRKEKEIAENEERRKEKLKQEKELSRQAQLEMKLKCKKDICEEPSDGDDVISLLIKFPCGKRINRRFHKAVSVKVLHDYVYAQDEAPARYQIFTSFPRAPIAGCSTDEVISTQSHPLIQDAGLNRNDTVFVQNVGEDSSSDDEDR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2021-06-02" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>F7J187</accession>
    <accession>A0A1W2W3G7</accession>
    <accession>F6QUZ6</accession>
    <name>NVD2_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName>Cholesterol 7-desaturase nvd 2</fullName>
            <ecNumber evidence="3">1.14.19.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Neverland 2</fullName>
            <shortName evidence="4">Nvd-Ci-2</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">nvd-Ci-2</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2011" name="J. Biol. Chem." volume="286" first="25756" last="25762">
            <title>The conserved Rieske oxygenase DAF-36/Neverland is a novel cholesterol-metabolizing enzyme.</title>
            <authorList>
                <person name="Yoshiyama-Yanagawa T."/>
                <person name="Enya S."/>
                <person name="Shimada-Niwa Y."/>
                <person name="Yaguchi S."/>
                <person name="Haramoto Y."/>
                <person name="Matsuya T."/>
                <person name="Shiomi K."/>
                <person name="Sasakura Y."/>
                <person name="Takahashi S."/>
                <person name="Asashima M."/>
                <person name="Kataoka H."/>
                <person name="Niwa R."/>
            </authorList>
            <dbReference type="PubMed" id="21632547"/>
            <dbReference type="DOI" id="10.1074/jbc.M111.244384"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [MRNA]</scope>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>PATHWAY</scope>
        <source>
            <tissue evidence="7">Embryo</tissue>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the production of 7-dehydrocholesterol (7-DHC or cholesta-5,7-dien-3beta-ol) by inserting a double bond (desaturating) at the C7-C8 single bond of cholesterol. Essential regulator of steroid biosynthesis as this reaction is the first step in the synthesis of the steroid hormone Delta(7)-dafachronic acid.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>cholesterol + NADPH + O2 + H(+) = 7-dehydrocholesterol + NADP(+) + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:45024"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16113"/>
            <dbReference type="ChEBI" id="CHEBI:17759"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.14.19.21"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:45025"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>cholesterol + NADH + O2 + H(+) = 7-dehydrocholesterol + NAD(+) + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:51644"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16113"/>
            <dbReference type="ChEBI" id="CHEBI:17759"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.14.19.21"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:51645"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
        <text evidence="2">Binds 1 [2Fe-2S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Steroid hormone biosynthesis; dafachronic acid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the cholesterol 7-desaturase family.</text>
    </comment>
    <comment type="sequence caution" evidence="5">
        <conflict type="erroneous gene model prediction">
            <sequence resource="EMBL" id="EAAA01003003"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="1.14.19.21" evidence="3"/>
    <dbReference type="EMBL" id="AB607953">
        <property type="protein sequence ID" value="BAK39962.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="EAAA01003003">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001265912.1">
        <property type="nucleotide sequence ID" value="NM_001278983.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F7J187"/>
    <dbReference type="SMR" id="F7J187"/>
    <dbReference type="STRING" id="7719.ENSCINP00000013559"/>
    <dbReference type="SwissLipids" id="SLP:000001122"/>
    <dbReference type="GeneID" id="100176071"/>
    <dbReference type="KEGG" id="cin:100176071"/>
    <dbReference type="eggNOG" id="ENOG502QS20">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037178_0_0_1"/>
    <dbReference type="InParanoid" id="F7J187"/>
    <dbReference type="OrthoDB" id="426882at2759"/>
    <dbReference type="UniPathway" id="UPA01020"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0170056">
        <property type="term" value="F:cholesterol 7-desaturase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008203">
        <property type="term" value="P:cholesterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03469">
        <property type="entry name" value="Rieske_RO_Alpha_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.380.10">
        <property type="entry name" value="Naphthalene 1,2-dioxygenase Alpha Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.102.10.10">
        <property type="entry name" value="Rieske [2Fe-2S] iron-sulphur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050584">
        <property type="entry name" value="Cholesterol_7-desaturase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045605">
        <property type="entry name" value="KshA-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036922">
        <property type="entry name" value="Rieske_2Fe-2S_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21266:SF32">
        <property type="entry name" value="CHOLESTEROL 7-DESATURASE NVD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21266">
        <property type="entry name" value="IRON-SULFUR DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19298">
        <property type="entry name" value="KshA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00355">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50022">
        <property type="entry name" value="ISP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51296">
        <property type="entry name" value="RIESKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0001">2Fe-2S</keyword>
    <keyword id="KW-0153">Cholesterol metabolism</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0443">Lipid metabolism</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0753">Steroid metabolism</keyword>
    <keyword id="KW-1207">Sterol metabolism</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000452608" description="Cholesterol 7-desaturase nvd 2">
        <location>
            <begin position="1"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="Rieske" evidence="2">
        <location>
            <begin position="107"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; BAK39962." ref="1">
        <original>K</original>
        <variation>E</variation>
        <location>
            <position position="117"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; BAK39962." ref="1">
        <original>L</original>
        <variation>G</variation>
        <location>
            <position position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="21632547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="4">
        <source>
            <dbReference type="PubMed" id="21632547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="5"/>
    <evidence type="ECO:0000305" key="6">
        <source>
            <dbReference type="PubMed" id="21632547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000312" key="7">
        <source>
            <dbReference type="EMBL" id="BAK39962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAK39962.1"/>
        </source>
    </evidence>
    <sequence length="452" mass="51977" checksum="020B5E55C7AB3354" modified="2021-06-02" version="2">MLIEYLIRITVMVITSERLLILMGLCDESFHFPVMIRVVFNAAVAIVIALVMSKLYKVLFAPLDLRRKLEDVGYVHHDHSVSREENIRDTQRRKKLGNTPPVFPNGWFKVADSTWIKKGQVKSIYFFGEQLALFRNKRGLLRALDAYCPHLLANMAAGGKVVNSDCLECPFHGWKFSGETGKLVDVPYAQKVPTFVSVKKWSCCEVDGMAYLWYHCDGGEPKWVLPSSVTINTLKYAGKTEHIINSHIQDIPENAADISHLDHLHKPVIGSDVEKTNESLLNNLIFHSIQASWKPPTDPNEPHRSTMSIKDNIHLSIFNIKIPLLYLEFEIDQIGPGAVHIHVRTPFFRGLMLQNVTPIEPFVQKLTHSFYVSPWVPVCIAKAFYLLETTQIERDILMWNNKTYFRQPVLVKEESALAKHRRWYQQFYSENSPRMNHRGDLVYPGKPKLADW</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>H2XJF4</accession>
    <name>H2XJF4_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Serine hydroxymethyltransferase</fullName>
            <ecNumber evidence="10">2.1.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100187164</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3 10">Interconversion of serine and glycine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + glycine + H2O = (6S)-5,6,7,8-tetrahydrofolate + L-serine</text>
            <dbReference type="Rhea" id="RHEA:15481"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="EC" id="2.1.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 9 10">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 10">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 10">Belongs to the SHMT family.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.1" evidence="10"/>
    <dbReference type="AlphaFoldDB" id="H2XJF4"/>
    <dbReference type="FunCoup" id="H2XJF4">
        <property type="interactions" value="13"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000016753"/>
    <dbReference type="Ensembl" id="ENSCINT00000016753.3">
        <property type="protein sequence ID" value="ENSCINP00000016753.3"/>
        <property type="gene ID" value="ENSCING00000008186.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000002762"/>
    <dbReference type="HOGENOM" id="CLU_022477_0_1_1"/>
    <dbReference type="InParanoid" id="H2XJF4"/>
    <dbReference type="OMA" id="VTNRNAI"/>
    <dbReference type="UniPathway" id="UPA00193"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004372">
        <property type="term" value="F:glycine hydroxymethyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019264">
        <property type="term" value="P:glycine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046653">
        <property type="term" value="P:tetrahydrofolate metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00378">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000050">
        <property type="entry name" value="Serine hydroxymethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00051">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001085">
        <property type="entry name" value="Ser_HO-MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049943">
        <property type="entry name" value="Ser_HO-MeTrfase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019798">
        <property type="entry name" value="Ser_HO-MeTrfase_PLP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039429">
        <property type="entry name" value="SHMT-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000586">
        <property type="entry name" value="PRK00011.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680">
        <property type="entry name" value="SERINE HYDROXYMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680:SF59">
        <property type="entry name" value="SERINE HYDROXYMETHYLTRANSFERASE, CYTOSOLIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00464">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000412">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00096">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="8 9 10" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Serine hydroxymethyltransferase-like" evidence="11">
        <location>
            <begin position="21"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="9">
        <location>
            <position position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00002224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000412-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000016753.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="478" mass="52546" checksum="620AE5D3561DD389" modified="2012-04-18" version="1">MSCGDTKPTETKESAWLEQPLEENDPEIYRIIRNEKERQRDGLELIASENFTSGAVLEALGSCLNNKYSEGYPGVRYYGGTENIDELERLCQKRALEVFKLNPEEWGVNVQPYSGSPANFAVLTAIVEPHGRIMGLDLPDGGHLTHGFMTEKKKISATSIFFESMPYKVNPATGLIDYDQLEQNAKLFKPKVIIAGMSCYSRVIDYERIRKIADANKALVMADMAHVSGLVATGVIPSPFEHCQIVTSTTHKTLRGPRAGIIFYRRGVKVPATDGKPAEMYNFEKPINEAVFPGLQGGPHNHAIAGVAVCLLQAKSPMFIEYQKNVVSNAQTLGKVLMDKGYDVVTGGTDTHLILVNLKSKGTDGNRADKVLEAIGVACNKNTCPGDKAALRPSGLRLGSPALTSRGLNGKDFEKVADFIDRGVQLTVEIQNSLEPKATFKDFRVKLYNDDVIIGKVKALKEEVTMFARTFPIPGLKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>H2XK24</accession>
    <name>H2XK24_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
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                <person name="Hastings K.E."/>
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                <person name="Huang W."/>
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                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
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                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
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                <person name="Doyle S."/>
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                <person name="Kobayashi K."/>
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                <person name="Manohar C."/>
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                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
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                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
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                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
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                <person name="Manohar C."/>
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                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
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                <person name="Miura S."/>
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                <person name="Nishizaka S."/>
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                <person name="Oishi K."/>
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                <person name="Richardson P."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="7" key="3">
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
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                <person name="DeSantis R."/>
                <person name="Doyle S."/>
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                <person name="Inaba K."/>
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                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
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                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
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                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>IDENTIFICATION</scope>
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            <authorList>
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        <scope>IDENTIFICATION</scope>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
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                <person name="Di Gregorio A."/>
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                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
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                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Putnam N."/>
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                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
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                <person name="Hayashi H."/>
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                <person name="Suzuki M.M."/>
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                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
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                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
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                <person name="Doggett N."/>
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                <person name="Richardson P."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
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                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
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                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
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                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
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            </authorList>
            <dbReference type="PubMed" id="18854010"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="6">wild type</strain>
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    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
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                <person name="Hastings K.E."/>
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                <person name="Lemaire P."/>
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                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
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                <person name="Putnam N."/>
                <person name="Rash S."/>
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                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
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                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">wild type</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the sulfotransferase 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="6"/>
    <dbReference type="EMBL" id="EAAA01000380">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="EAAA01000381">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XKU8"/>
    <dbReference type="STRING" id="7719.ENSCINP00000030280"/>
    <dbReference type="Ensembl" id="ENSCINT00000035346.1">
        <property type="protein sequence ID" value="ENSCINP00000030280.1"/>
        <property type="gene ID" value="ENSCING00000025091.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158991"/>
    <dbReference type="HOGENOM" id="CLU_017703_0_0_1"/>
    <dbReference type="InParanoid" id="H2XKU8"/>
    <dbReference type="OMA" id="HMQNWLQ"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008467">
        <property type="term" value="F:[heparan sulfate]-glucosamine 3-sulfotransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037359">
        <property type="entry name" value="NST/OST"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10605:SF65">
        <property type="entry name" value="GH20068P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10605">
        <property type="entry name" value="HEPARAN SULFATE SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Sulfotransferase" evidence="7">
        <location>
            <begin position="75"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="active site" description="For sulfotransferase activity" evidence="3">
        <location>
            <position position="84"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="84"/>
            <end position="88"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="271"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="286"/>
            <end position="290"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="272"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR637359-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR637359-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR637359-3"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
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            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000030280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="322" mass="37476" checksum="A8B86FB654F68711" modified="2012-04-18" version="1">MMQKQLRIINYLVIVGIVYRNITFYKSKLWDSYHTSVDNIVDILFDAVADENYCLHGGPCVPKNYVSMYKSQSYPDAIIIGCKKCGTRALIDMMCLHPQIKALRREGHYFDDFYALGDYWYKSRMPILADHEVAIEKTPSYMLSRLTAERIYNFNPDIKLIVILRNPVDRLISDYLQKQARFPKAVVLSVGETYIKNKTNTVDETKVHVQKGLYAKQLKPWLEIFGTKSILIEDGNAFTKDPLPTLKRAQQFLGLKPHNNHVYFNKSKGFYCWLENSQTKCLAGAKGRKHPKIPSQQMNILKSYYTKPNEDLFKMIGKRFDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>H2XKV6</accession>
    <name>H2XKV6_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">wild type</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01001864">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XKV6"/>
    <dbReference type="STRING" id="7719.ENSCINP00000030288"/>
    <dbReference type="Ensembl" id="ENSCINT00000031892.1">
        <property type="protein sequence ID" value="ENSCINP00000030288.1"/>
        <property type="gene ID" value="ENSCING00000024837.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158366"/>
    <dbReference type="HOGENOM" id="CLU_008163_1_2_1"/>
    <dbReference type="InParanoid" id="H2XKV6"/>
    <dbReference type="OMA" id="GRTCHAN"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000241">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050778">
        <property type="entry name" value="Cueball_EGF_LRP_Nidogen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000033">
        <property type="entry name" value="LDLR_classB_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46513:SF44">
        <property type="entry name" value="LDL RECEPTOR RELATED PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46513">
        <property type="entry name" value="VITELLOGENIN RECEPTOR-LIKE PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00058">
        <property type="entry name" value="Ldl_recept_b"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00135">
        <property type="entry name" value="LY"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63825">
        <property type="entry name" value="YWTD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51120">
        <property type="entry name" value="LDLRB"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="repeat" description="LDL-receptor class B" evidence="6">
        <location>
            <begin position="84"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="6">
        <location>
            <begin position="127"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="6">
        <location>
            <begin position="170"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000030288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="222" mass="24857" checksum="D794C6F72E6D7ECA" modified="2012-04-18" version="1">RCEQAHCSHLCLPRPDGITCACPTGFRRVNITHCAEDINHFLVFTRKTDIRRISFDGVERADTVLPVKNLKNAVALDWHSDHPGHIYWSDVSKDTISRAEWDGSQEKVIVSSSLDSPAGLAIDWITNKIYWTEAGINRIEVALLDGSMRCVLIWTGLDRPRAIVVHPKTGHMIWSDWGAEPKIERAGMDGTNRTTLVSTGLTWPNGVAIDYTSDMIYWTDAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>H2XM24</accession>
    <name>H2XM24_CIOIN</name>
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            <fullName evidence="2">Fibrinogen C-terminal domain-containing protein</fullName>
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        <name type="scientific">Ciona intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
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    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">wild type</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Putnam N."/>
                <person name="Rash S."/>
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                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
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                <person name="Mochizuki Y."/>
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                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
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                <person name="Yoshizaki F."/>
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                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
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                <person name="Detter C."/>
                <person name="Doggett N."/>
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                <person name="Richardson P."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4">wild type</strain>
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    <reference evidence="4" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <property type="term" value="F:G-protein beta/gamma-subunit complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000692">
        <property type="entry name" value="Guanine nucleotide-binding protein subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001019">
        <property type="entry name" value="Gprotein_alpha_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011025">
        <property type="entry name" value="GproteinA_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218">
        <property type="entry name" value="GTP-BINDING PROTEIN ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218:SF302">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN ALPHA-5 SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00503">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47895">
        <property type="entry name" value="Transducin (alpha subunit), insertion domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51882">
        <property type="entry name" value="G_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0807">Transducer</keyword>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="46"/>
            <end position="51"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000031542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="119" mass="13137" checksum="A9FE94B7E1B00733" modified="2012-04-18" version="1">MLKMGCGLSKDNAEAGKISRDIDRQIKTETKAFQEQIKLLLLGAGESGKSTIVKQMVIIHKNGFSEEEVYKFRPIVFGNVLQCMLSMVVAMNKLGIQYEDKERENDAGKLLAAAAANTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>H2XQ09</accession>
    <name>H2XQ09_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Sulfotransferase</fullName>
            <ecNumber evidence="1">2.8.2.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sulfotransferase 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="1"/>
    <dbReference type="EMBL" id="EAAA01001803">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XQ09"/>
    <dbReference type="Ensembl" id="ENSCINT00000030961.1">
        <property type="protein sequence ID" value="ENSCINP00000031743.1"/>
        <property type="gene ID" value="ENSCING00000023820.1"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2941014_0_0_1"/>
    <dbReference type="InParanoid" id="H2XQ09"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Sulfotransferase" evidence="2">
        <location>
            <begin position="9"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU361155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000031743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="60" mass="6991" checksum="78EC5C4FBC6B969C" modified="2012-04-18" version="1">MKSRNIWEHPENEPFIRKGVIGDWKNHLSAEQADGLDRALMEHGKDGGILKLWNKYAEFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>H2XQD7</accession>
    <name>H2XQD7_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histone deacetylase complex subunit SAP18</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">18 kDa Sin3-associated polypeptide</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Sin3-associated polypeptide p18</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC104266248</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">wild type</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="7">Found in a mRNA splicing-dependent exon junction complex (EJC). Component of the heterotrimeric ASAP (apoptosis- and splicing-associated protein) and PSAP complexes consisting of RNPS1, SAP18 and either ACIN1 or PNN, respectively; the ASAP and PSAP complexes probably are formed mutually exclusive. For the ASAP complex, the association of SAP18 seems to require a preformed RNPS1:ACIN1 complex. Forms a complex with SIN3A and HDAC1. Interacts with SUFU.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SAP18 family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01000832">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XQD7"/>
    <dbReference type="FunCoup" id="H2XQD7">
        <property type="interactions" value="859"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000031871"/>
    <dbReference type="Ensembl" id="ENSCINT00000033785.1">
        <property type="protein sequence ID" value="ENSCINP00000031871.1"/>
        <property type="gene ID" value="ENSCING00000003322.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000003152"/>
    <dbReference type="InParanoid" id="H2XQD7"/>
    <dbReference type="OMA" id="TYRMREI"/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
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                <person name="Mochizuki Y."/>
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                <person name="Tassy O."/>
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                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
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                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
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                <person name="Richardson P."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
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                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">wild type</strain>
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            <authorList>
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        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
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                <person name="Di Gregorio A."/>
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                <person name="Hastings K.E."/>
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                <person name="Kawashima T."/>
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                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
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                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
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                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
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                <person name="Wiley G.B."/>
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                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
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                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
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                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
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                <person name="Wada S."/>
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                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">wild type</strain>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satake M."/>
                <person name="Terry A."/>
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                <person name="Wang H.G."/>
                <person name="Awazu S."/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
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                <person name="Francino P."/>
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                <person name="Hayashi H."/>
                <person name="Hino K."/>
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                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
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                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">wild type</strain>
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        <scope>IDENTIFICATION</scope>
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                <consortium name="Ensembl"/>
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            <taxon>Eukaryota</taxon>
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            <taxon>Phlebobranchia</taxon>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">wild type</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="gene ID" value="ENSCING00000021372.1"/>
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    <dbReference type="HOGENOM" id="CLU_3407972_0_0_1"/>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000033828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
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<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>H2XW46</accession>
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            <fullName evidence="5">Ceramide transfer protein</fullName>
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            <fullName evidence="12">Collagen type IV alpha-3-binding protein</fullName>
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        <name type="scientific">Ciona intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
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                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
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                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
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                <person name="Mount S."/>
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                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
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                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
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            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">wild type</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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            <text>N-hexadecanoylsphing-4-enine(in) = N-hexadecanoylsphing-4-enine(out)</text>
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            <dbReference type="ChEBI" id="CHEBI:72959"/>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
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        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
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        <subcellularLocation>
            <location evidence="4">Golgi apparatus</location>
        </subcellularLocation>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="H2XW46"/>
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    <dbReference type="OMA" id="LETCHRI"/>
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    <dbReference type="GO" id="GO:0005783">
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        <property type="project" value="GO_Central"/>
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    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000033880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="529" mass="60856" checksum="2C5470F715FBAF7C" modified="2012-04-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>H2XY66</accession>
    <name>H2XY66_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="H2XY66"/>
    <dbReference type="Ensembl" id="ENSCINT00000033782.1">
        <property type="protein sequence ID" value="ENSCINP00000034600.1"/>
        <property type="gene ID" value="ENSCING00000020764.1"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2855452_0_0_1"/>
    <dbReference type="InParanoid" id="H2XY66"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000034600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="65" mass="7399" checksum="444762E651ED12B2" modified="2012-04-18" version="1">KQPTKTEKVFHIAGGTSCVNLKQFQTYHYQLIIVCLVSFSWAPCVGNKTDKIRTKSLLKRGHDSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>H2XZC7</accession>
    <name>H2XZC7_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Plastocyanin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the multicopper oxidase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="H2XZC7"/>
    <dbReference type="STRING" id="7719.ENSCINP00000035011"/>
    <dbReference type="Ensembl" id="ENSCINT00000034853.1">
        <property type="protein sequence ID" value="ENSCINP00000035011.1"/>
        <property type="gene ID" value="ENSCING00000025155.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00440000034776"/>
    <dbReference type="HOGENOM" id="CLU_1690700_0_0_1"/>
    <dbReference type="InParanoid" id="H2XZC7"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13853">
        <property type="entry name" value="CuRO_1_Tth-MCO_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF518">
        <property type="entry name" value="MULTICOPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Plastocyanin-like" evidence="2">
        <location>
            <begin position="43"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000035011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="156" mass="17989" checksum="76250C2220A099D1" modified="2012-04-18" version="1">MFKGGELLRQPQQHISSNGELSLTYNVRMADFQVEWLTLHRRTYNGEIPGATWRIKRGDKVNLHLNNTLTEPEKLQPFNGFRFTNSTNIHTHGLHISSREPEDNIFVHLKPGQSYNYKYNIDPDNPSGTYWYHSHLHGASLFQVHSGMAGMIIVDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>H2XZF2</accession>
    <name>H2XZF2_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">TIR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Toll-like receptor family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="H2XZF2"/>
    <dbReference type="STRING" id="7719.ENSCINP00000035036"/>
    <dbReference type="Ensembl" id="ENSCINT00000030271.1">
        <property type="protein sequence ID" value="ENSCINP00000035036.1"/>
        <property type="gene ID" value="ENSCING00000024511.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159318"/>
    <dbReference type="HOGENOM" id="CLU_332510_0_0_1"/>
    <dbReference type="InParanoid" id="H2XZF2"/>
    <dbReference type="OMA" id="CENDEAP"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
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                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
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                <person name="Miura S."/>
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                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
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                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4">wild type</strain>
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    <reference evidence="4" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
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                <person name="Hastings K.E."/>
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                <person name="Putnam N."/>
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                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
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                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
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                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
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                <person name="Kobayashi K."/>
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                <person name="Sasakura Y."/>
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                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="H2Y0H9"/>
    <dbReference type="Ensembl" id="ENSCINT00000036737.1">
        <property type="protein sequence ID" value="ENSCINP00000035413.1"/>
        <property type="gene ID" value="ENSCING00000019642.1"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2474569_0_0_1"/>
    <dbReference type="InParanoid" id="H2Y0H9"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000035413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="88" mass="8959" checksum="52AA50EBBDF86D12" modified="2012-04-18" version="1">SLVDDVREIKQLLQSIAPGNLNEFGSQTSGRVGSDGGGGIKQKSSLLHQVVSAVSGQGANMQSLSHTVESELISLPGSVPATSSDSHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>H2Y1L8</accession>
    <name>H2Y1L8_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">wild type</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01000814">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2Y1L8"/>
    <dbReference type="Ensembl" id="ENSCINT00000035098.1">
        <property type="protein sequence ID" value="ENSCINP00000035802.1"/>
        <property type="gene ID" value="ENSCING00000023544.1"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1834441_0_0_1"/>
    <dbReference type="InParanoid" id="H2Y1L8"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000035802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="140" mass="15745" checksum="1B2D6AC42FCCBFD5" modified="2012-04-18" version="1">MQHEALLRHNPIHAWSDVTSSSSEGDSPKQQPVNFNKRYLKTMTSSSSLISRDACLSSYYVTYDRRLLSRGAVELAITAEEGTISVSILQVKDIPIDFIQRASLLLKLSLNTDKRKKLQFRTRSVPVTSSKISFNETLAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>H2Y231</accession>
    <name>H2Y231_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="H2Y231"/>
    <dbReference type="Ensembl" id="ENSCINT00000037263.1">
        <property type="protein sequence ID" value="ENSCINP00000035966.1"/>
        <property type="gene ID" value="ENSCING00000019026.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000169530"/>
    <dbReference type="HOGENOM" id="CLU_162103_0_0_1"/>
    <dbReference type="InParanoid" id="H2Y231"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000035966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="126" mass="14180" checksum="2782ED87EB1E4828" modified="2012-04-18" version="1">MEWRSEVTNMHPGEEKRILAKQTFEGLLFSTNNILPLTKQLLHYIDGVPLGNLTQDCLEAMFGNLRQYMHRNTNPDISQVGYGINALSKRRIISKIKGGNTTGSHGAINAWTKVCNDPLGKRKKKT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1990-04-01" modified="2025-02-05" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>P16240</accession>
    <name>CION_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName>Cionin</fullName>
        </recommendedName>
    </protein>
    <organism>
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1993" name="FEBS Lett." volume="331" first="60" last="64">
            <title>cDNA deduced procionin. Structure and expression in protochordates resemble that of procholecystokinin in mammals.</title>
            <authorList>
                <person name="Monstein H.J."/>
                <person name="Thorup J.U."/>
                <person name="Folkesson R."/>
                <person name="Johnsen A.H."/>
                <person name="Rehfeld J.F."/>
            </authorList>
            <dbReference type="PubMed" id="8405412"/>
            <dbReference type="DOI" id="10.1016/0014-5793(93)80297-8"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [MRNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1990" name="J. Biol. Chem." volume="265" first="3054" last="3058">
            <title>Cionin: a disulfotyrosyl hybrid of cholecystokinin and gastrin from the neural ganglion of the protochordate Ciona intestinalis.</title>
            <authorList>
                <person name="Johnsen A.H."/>
                <person name="Rehfeld J.F."/>
            </authorList>
            <dbReference type="PubMed" id="2303439"/>
        </citation>
        <scope>PROTEIN SEQUENCE OF 109-116</scope>
        <scope>SULFATION AT TYR-110 AND TYR-111</scope>
        <scope>AMIDATION AT PHE-116</scope>
        <source>
            <tissue>Neural ganglion</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="tissue specificity">
        <text>Expressed in both the gut and the neural ganglion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the gastrin/cholecystokinin family.</text>
    </comment>
    <dbReference type="EMBL" id="X69130">
        <property type="protein sequence ID" value="CAA48884.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="PIR" id="S36901">
        <property type="entry name" value="S36901"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001027711.1">
        <property type="nucleotide sequence ID" value="NM_001032539.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P16240"/>
    <dbReference type="GeneID" id="445737"/>
    <dbReference type="InParanoid" id="P16240"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005179">
        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007218">
        <property type="term" value="P:neuropeptide signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039236">
        <property type="entry name" value="GAST"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001651">
        <property type="entry name" value="Gastrin/CCK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013152">
        <property type="entry name" value="Gastrin/cholecystokinin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19309">
        <property type="entry name" value="GASTRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19309:SF0">
        <property type="entry name" value="GASTRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00918">
        <property type="entry name" value="Gastrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00259">
        <property type="entry name" value="GASTRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0027">Amidation</keyword>
    <keyword id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword id="KW-0903">Direct protein sequencing</keyword>
    <keyword id="KW-0372">Hormone</keyword>
    <keyword id="KW-0527">Neuropeptide</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0964">Secreted</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <keyword id="KW-0765">Sulfation</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="propeptide" id="PRO_0000010671" evidence="2">
        <location>
            <begin position="23"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="peptide" id="PRO_0000010672" description="Cionin">
        <location>
            <begin position="109"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="propeptide" id="PRO_0000010673">
        <location>
            <begin position="120"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="modified residue" description="Sulfotyrosine" evidence="2">
        <location>
            <position position="110"/>
        </location>
    </feature>
    <feature type="modified residue" description="Sulfotyrosine" evidence="2">
        <location>
            <position position="111"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phenylalanine amide" evidence="2">
        <location>
            <position position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="2303439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="128" mass="14820" checksum="BF1DE73438E66609" modified="1994-06-01" version="2" precursor="true">MGSNIVIYFSIIVIVTLNVNGVPASDLFKSVSQYHIPRSKVINKETVTKPLQFQRAICRLLQKLGEETFARLSQSELEAKQLDLIKTCYQANSFGDNENQGHMQRMDRNYYGWMDFGKRAIEDVDYEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-16" modified="2026-01-28" version="130" xmlns="https://uniprot.org/uniprot">
    <accession>Q1RLI9</accession>
    <accession>A0A1W2VRF3</accession>
    <accession>F6ZG43</accession>
    <name>Q1RLI9_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">Ci-ZF(Bbox/RING)-1</name>
        <name evidence="16" type="synonym">zf(bbox/ring)-1</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2006" name="Dev. Biol." volume="292" first="546" last="554">
            <title>Systematic analysis of embryonic expression profiles of zinc finger genes in Ciona intestinalis.</title>
            <authorList>
                <person name="Miwata K."/>
                <person name="Chiba T."/>
                <person name="Horii R."/>
                <person name="Yamada L."/>
                <person name="Kubo A."/>
                <person name="Miyamura D."/>
                <person name="Satoh N."/>
                <person name="Satou Y."/>
            </authorList>
            <dbReference type="PubMed" id="16519883"/>
            <dbReference type="DOI" id="10.1016/j.ydbio.2006.01.024"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">wild type</strain>
        </source>
    </reference>
    <reference evidence="16" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRIM/RBCC family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="3"/>
    <dbReference type="EMBL" id="EAAA01002611">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="BR000045">
        <property type="protein sequence ID" value="FAA00076.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001122371.1">
        <property type="nucleotide sequence ID" value="NM_001128899.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1RLI9"/>
    <dbReference type="STRING" id="7719.ENSCINP00000024518"/>
    <dbReference type="Ensembl" id="ENSCINT00000024764.2">
        <property type="protein sequence ID" value="ENSCINP00000024518.2"/>
        <property type="gene ID" value="ENSCING00000013322.2"/>
    </dbReference>
    <dbReference type="GeneID" id="100169918"/>
    <dbReference type="KEGG" id="cin:100169918"/>
    <dbReference type="CTD" id="100169918"/>
    <dbReference type="GeneTree" id="ENSGT00940000162489"/>
    <dbReference type="HOGENOM" id="CLU_337052_0_0_1"/>
    <dbReference type="OMA" id="QFNEPRY"/>
    <dbReference type="OrthoDB" id="6265224at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19757">
        <property type="entry name" value="Bbox1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19756">
        <property type="entry name" value="Bbox2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05819">
        <property type="entry name" value="NHL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.830.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001258">
        <property type="entry name" value="NHL_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047153">
        <property type="entry name" value="TRIM45/56/19-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25462">
        <property type="entry name" value="BONUS, ISOFORM C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25462:SF229">
        <property type="entry name" value="TRANSCRIPTION INTERMEDIARY FACTOR 1-BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01436">
        <property type="entry name" value="NHL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101898">
        <property type="entry name" value="NHL repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51125">
        <property type="entry name" value="NHL"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="13">
        <location>
            <begin position="45"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="14">
        <location>
            <begin position="125"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="14">
        <location>
            <begin position="189"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="repeat" description="NHL" evidence="10">
        <location>
            <begin position="532"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="repeat" description="NHL" evidence="10">
        <location>
            <begin position="579"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="repeat" description="NHL" evidence="10">
        <location>
            <begin position="626"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="760"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="231"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="764"/>
            <end position="780"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="FAA00076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000024518.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="845" mass="93781" checksum="B7CF8E6AE48DB5FA" modified="2006-05-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2005-08-30" modified="2026-01-28" version="110" xmlns="https://uniprot.org/uniprot">
    <accession>Q4H2R5</accession>
    <accession>A0A1W2VP49</accession>
    <name>Q4H2R5_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">Transcription elongation factor SPT4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">Ci-SPT4</name>
        <name evidence="15" type="synonym">spt4</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
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                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <title>Genomewide surveys of developmentally relevant genes in Ciona intestinalis.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Satoh N."/>
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            <dbReference type="DOI" id="10.1007/s00427-003-0330-z"/>
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        <citation type="journal article" date="2004" name="Development" volume="131" first="4047" last="4058">
            <title>Gene expression profiles of transcription factors and signaling molecules in the ascidian embryo: towards a comprehensive understanding of gene networks.</title>
            <authorList>
                <person name="Imai K.S."/>
                <person name="Hino K."/>
                <person name="Yagi K."/>
                <person name="Satoh N."/>
                <person name="Satou Y."/>
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            <dbReference type="DOI" id="10.1242/dev.01270"/>
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    <reference evidence="14" key="4">
        <citation type="submission" date="2005-04" db="EMBL/GenBank/DDBJ databases">
            <title>Expressed genes in Ciona intestinalis.</title>
            <authorList>
                <person name="Satou Y."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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        <text evidence="12">Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates transcription elongation by RNA polymerase II.</text>
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            <location evidence="1 12">Nucleus</location>
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    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the SPT4 family.</text>
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            <title>Further Characterization of Brachyury-Downstream Genes in Ciona intestinalis Embryo.</title>
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                <person name="Takahashi H."/>
                <person name="Satoh N."/>
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    <reference evidence="9" key="2">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
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                <person name="Richardson P."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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    <dbReference type="PANTHER" id="PTHR14604">
        <property type="entry name" value="WD40 REPEAT PF20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12125">
        <property type="entry name" value="Beta-TrCP_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM01028">
        <property type="entry name" value="Beta-TrCP_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="1 4" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="F-box" evidence="6">
        <location>
            <begin position="208"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="315"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="355"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="395"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="438"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="478"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="518"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="567"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="22"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAC57516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000018885.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="621" mass="70792" checksum="6D176B121959F8C3" modified="2003-06-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2025-06-18" version="117" xmlns="https://uniprot.org/uniprot">
    <accession>Q94425</accession>
    <name>SYIM_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName>Probable isoleucine--tRNA ligase, mitochondrial</fullName>
            <ecNumber>6.1.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Isoleucyl-tRNA synthetase</fullName>
            <shortName>IleRS</shortName>
        </alternativeName>
    </protein>
    <organism>
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="1996-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Bird A.P."/>
                <person name="Clark V."/>
                <person name="Jones S.J.M."/>
                <person name="Leitgeb S."/>
                <person name="Lennard N."/>
                <person name="Tweedie S."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>tRNA(Ile) + L-isoleucine + ATP = L-isoleucyl-tRNA(Ile) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:9666"/>
            <dbReference type="Rhea" id="RHEA-COMP:9695"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58045"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78528"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.5"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.5"/>
    <dbReference type="EMBL" id="Z80904">
        <property type="protein sequence ID" value="CAB02584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="T31663">
        <property type="entry name" value="T31663"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q94425"/>
    <dbReference type="SMR" id="Q94425"/>
    <dbReference type="STRING" id="7719.ENSCINP00000017328"/>
    <dbReference type="eggNOG" id="KOG0433">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="Q94425"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004822">
        <property type="term" value="F:isoleucine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006428">
        <property type="term" value="P:isoleucyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000128">
        <property type="entry name" value="Isoleucyl-tRNA synthetase 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.830">
        <property type="entry name" value="Ile-tRNA synthetase CP2 domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002301">
        <property type="entry name" value="Ile-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050081">
        <property type="entry name" value="Ile-tRNA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42765:SF1">
        <property type="entry name" value="ISOLEUCINE--TRNA LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42765">
        <property type="entry name" value="SOLEUCYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00984">
        <property type="entry name" value="TRNASYNTHILE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="ValRS/IleRS/LeuRS editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0496">Mitochondrion</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000098600" description="Probable isoleucine--tRNA ligase, mitochondrial">
        <location>
            <begin position="1" status="less than"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region">
        <location>
            <begin position="298"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="301"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="413" mass="46895" checksum="69B098FD71C100CE" modified="1997-02-01" version="1" fragment="single">GMICRHPIYLERTVRMIGSDIANTSKGTGVVHIAPAHGMDDFGIGQKHNLSTESSVNSDGEFMANIAPNLEGKFIFTSGNEAVVEMLKEEMNLFHEHDYKHSYPYDWRTKQPIFVHTSNQWFVDTAMLANKAEAAISGITTYPDNGISSMLNRLQLRSYWCISRQRAWGLPIPVFYHKESGELLLNESTIQHLVSLVLKHGADIWWEGTELLPTQYNADEYERGRDILDIWFDSGVSWDCVLRDSKNRTTQADFYIEGKDQYGGWFQSSLLTSVGLQGVAPYKNLMVHGFVLDEKGQKMSKSIGNVVDPMVLTNGGKNPQRDPAYGADALRWWVAESNVHQDVHISTTIIKSAAESVQKIRNSVRFMLGNLNEFDKMKIVPTPAMLILDLRYRFEDKKLWYVTSMNYPLNLSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-06-07" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1W2WHS9</accession>
    <accession>H2XZ65</accession>
    <name>A0A1W2WHS9_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 11">Amidophosphoribosyltransferase</fullName>
            <shortName evidence="11">ATase</shortName>
            <ecNumber evidence="3 11">2.4.2.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9 11">Glutamine phosphoribosylpyrophosphate amidotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC100179384</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">wild type</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>5-phospho-beta-D-ribosylamine + L-glutamate + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + L-glutamine + H2O</text>
            <dbReference type="Rhea" id="RHEA:14905"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58681"/>
            <dbReference type="EC" id="2.4.2.14"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="10">
            <dbReference type="Rhea" id="RHEA:14907"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="13">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="14">Binds 1 [4Fe-4S] cluster per subunit.</text>
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    <comment type="pathway">
        <text evidence="1 11">Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2.</text>
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    <comment type="similarity">
        <text evidence="2 11">In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.</text>
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        <property type="nucleotide sequence ID" value="XM_002131344.5"/>
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        <property type="reaction ID" value="UER00124"/>
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        <property type="component" value="Chromosome 11"/>
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        <property type="entry name" value="Pribosyltran"/>
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    <keyword evidence="4 11" id="KW-0328">Glycosyltransferase</keyword>
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    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="13"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="12">
        <location>
            <position position="13"/>
        </location>
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        <location>
            <position position="272"/>
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        <ligand>
            <name>[4Fe-4S] cluster</name>
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        <location>
            <position position="319"/>
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        <ligand>
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        <ligand>
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            <dbReference type="Proteomes" id="UP000008144"/>
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    <sequence length="502" mass="54058" checksum="03DDDCC2A9D7BD59" modified="2017-06-07" version="1">MTSQMEDKLQDECGVFGCVAASNCTTPVDVANIISIGLIGLQHRGQESAGIVTNNASIDDGMRVHKGMGLVSAVFNPIILKQLDGNLGIGHTRYSTQGKSEIINCQPFVVDTKYGKIAVAHNGELVNKSKLRRDVLDRGVGLSTCSDSELITQLLCLPLDSEKSLEPDWTGRLRHLMLRTPASYSLVLLHGGAIYAARDPFGNRPLCIGRLEGDVGGKSPDTVGWIVSSESCVFQSTGAAYIGDVQPGQVVKVTNTGVTYTTAVNSGQSAFCIFEYVYFARPDSIFEGQTVYDVRRRCGAQLAREFPVDADVISTVPESATPAAFGFSRESGIPYDEVLAKNRYIGRTFIQPSTRMRKLAVEKKFGVLVENVRGKRIVVVDDSIVRGNTMGSIIRMLRSGGAAEVHVRIASPPVKNPCYMGINIPTPKELLANKLNLEETTKFLGADSLGYLSVKGLLSCVTSGRGKLVENGHSPETKSNGSCGTGHCVACLTGEYPVQLEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-06-07" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1W5B2W5</accession>
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    <name>A0A1W5B2W5_CIOIN</name>
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        <submittedName>
            <fullName evidence="3">3-oxoacyl-[acyl-carrier-protein] reductase FabG</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100183433</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001766">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_002126754.3"/>
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        <property type="nucleotide sequence ID" value="XM_018811268.2"/>
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        <property type="nucleotide sequence ID" value="XM_026833784.1"/>
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        <property type="protein sequence ID" value="ENSCINP00000020171.3"/>
        <property type="gene ID" value="ENSCING00000010051.3"/>
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    <dbReference type="KEGG" id="cin:100183433"/>
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    <dbReference type="GeneTree" id="ENSGT00940000173427"/>
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    <dbReference type="OMA" id="VNNVGHW"/>
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        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05233">
        <property type="entry name" value="SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000084">
        <property type="entry name" value="Short-chain dehydrogenase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
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    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
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        <property type="entry name" value="SDR_fam"/>
    </dbReference>
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        <property type="entry name" value="DEHYDROGENASES, SHORT CHAIN"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="ESTRADIOL 17-BETA-DEHYDROGENASE 8-RELATED"/>
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        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="GDHRDH"/>
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        <property type="entry name" value="SDRFAMILY"/>
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        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
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                <person name="Chapman J."/>
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                <person name="De Tomaso A."/>
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                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
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                <person name="Hastings K.E."/>
                <person name="Ho I."/>
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                <person name="Kawashima T."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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            <authorList>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">wild type</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Gfa family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01000538">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002130065.1">
        <property type="nucleotide sequence ID" value="XM_002130029.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6QAE6"/>
    <dbReference type="STRING" id="7719.ENSCINP00000006169"/>
    <dbReference type="Ensembl" id="ENSCINT00000006169.3">
        <property type="protein sequence ID" value="ENSCINP00000006169.3"/>
        <property type="gene ID" value="ENSCING00000013983.2"/>
    </dbReference>
    <dbReference type="GeneID" id="100182930"/>
    <dbReference type="KEGG" id="cin:100182930"/>
    <dbReference type="GeneTree" id="ENSGT00390000003183"/>
    <dbReference type="HOGENOM" id="CLU_055491_7_3_1"/>
    <dbReference type="InParanoid" id="F6QAE6"/>
    <dbReference type="OMA" id="DCSLCRR"/>
    <dbReference type="OrthoDB" id="2993351at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016846">
        <property type="term" value="F:carbon-sulfur lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052355">
        <property type="entry name" value="CENP-V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006913">
        <property type="entry name" value="CENP-V/GFA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011057">
        <property type="entry name" value="Mss4-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28620:SF1">
        <property type="entry name" value="CENP-V_GFA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28620">
        <property type="entry name" value="CENTROMERE PROTEIN V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04828">
        <property type="entry name" value="GFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51316">
        <property type="entry name" value="Mss4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51891">
        <property type="entry name" value="CENP_V_GFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CENP-V/GFA" evidence="4">
        <location>
            <begin position="8"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000006169.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="142" mass="15946" checksum="AA8F91A08292B930" modified="2012-04-18" version="2">MSLDLVKHTGGCHCGAVRFEVMETSIIYAIDCNCSICYMKSNAHFIVPSSSFKLLKGSENLTTYTFNTHQAKHTFCKICGVQSFYTPRSNTDGYGINPRCIDKGTIQKIISENFDGDKWEASMEKEKLKDAPITDRSKSKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>F6QAK1</accession>
    <name>F6QAK1_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Monoacylglycerol lipase ABHD2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100185844</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">wild type</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily. AB hydrolase 4 family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001796">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6QAK1"/>
    <dbReference type="FunCoup" id="F6QAK1">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000018138"/>
    <dbReference type="Ensembl" id="ENSCINT00000018138.3">
        <property type="protein sequence ID" value="ENSCINP00000018138.3"/>
        <property type="gene ID" value="ENSCING00000008924.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182902"/>
    <dbReference type="HOGENOM" id="CLU_032487_5_0_1"/>
    <dbReference type="InParanoid" id="F6QAK1"/>
    <dbReference type="OMA" id="HCTGEDV"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036126">
        <property type="term" value="C:sperm flagellum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097524">
        <property type="term" value="C:sperm plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008126">
        <property type="term" value="F:acetylesterase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047372">
        <property type="term" value="F:monoacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046464">
        <property type="term" value="P:acylglycerol catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051792">
        <property type="term" value="P:medium-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051793">
        <property type="term" value="P:medium-chain fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048240">
        <property type="term" value="P:sperm capacitation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043401">
        <property type="term" value="P:steroid hormone receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000880">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050960">
        <property type="entry name" value="AB_hydrolase_4_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012020">
        <property type="entry name" value="ABHD4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10794">
        <property type="entry name" value="ABHYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10794:SF45">
        <property type="entry name" value="MONOACYLGLYCEROL LIPASE ABHD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005211">
        <property type="entry name" value="Ab_hydro_YheT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="3">
        <location>
            <begin position="117"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="2">
        <location>
            <position position="197"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="2">
        <location>
            <position position="333"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="2">
        <location>
            <position position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR005211-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000018138.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="396" mass="44655" checksum="D08DC7222B729FF0" modified="2012-04-18" version="2">MLPVIAIGTVILLFVFIKFLRIHDETTRPKLHHSGSGFIKLLQKVISKCPSLTEIYQPPSLWGKCGHIQTAIYGKIGRIDCPQPNGKRHSITLATKETITFDVFQPVNGHHSSGECTILVCPGICNSSEKKYVRTFIHYAVNMGFRVAVLNHIGALPNVRLTSPKIFTYGETKYFDCMVQCVTSRYPTSRYVIVGFSMGANIVVRYLAEDRSRQLPIVCAVSWCQGYDGLRSTHALSSGFGRFYNYMVTMNFKKILKPHWNELFKVPTNNNDTKLSYDEKKVESATTLAEIEDGLLRHFDGFKSHVDYKALCGCAHMIDNIHIPILLLNSEDDPLIISDVHDIPISYSRKSEKGLFAAMQHGGHLGFYKGRWYKPDATTLLESITMEYIEAVLGHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>F6QBP3</accession>
    <name>F6QBP3_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01002928">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6QBP3"/>
    <dbReference type="Ensembl" id="ENSCINT00000016952.3">
        <property type="protein sequence ID" value="ENSCINP00000016952.3"/>
        <property type="gene ID" value="ENSCING00000008295.3"/>
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    <dbReference type="GeneTree" id="ENSGT00530000066547"/>
    <dbReference type="HOGENOM" id="CLU_1282844_0_0_1"/>
    <dbReference type="InParanoid" id="F6QBP3"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 9"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="181"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000016952.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="214" mass="23900" checksum="7F543D935E75E4FD" modified="2012-04-18" version="2">MAGEIFATSVRLGTIRLYPRFVDSGCNNNNIEIQLGLNEITFVATSAENEDDGKEIDIKIPVQAIHKVQAHLQGHCPVIWMNVNSACAAELRTLCGIQDKHGAYFDPDAIKFAERQISITSISTSDSRPFALQNLIKNYFFKIAKQIKIKPDTFFSSINQQQCNVKLMQVADAITKLGHQKKPRIPSTFGKGNETPKKPQLKRSKQSVSPLKEE</sequence>
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<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>F6QDM2</accession>
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        <recommendedName>
            <fullName evidence="2">glutathione transferase</fullName>
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    </protein>
    <organism evidence="5 6">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
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            <taxon>Eukaryota</taxon>
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    <reference evidence="6" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GST superfamily. Alpha family.</text>
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    <dbReference type="EC" id="2.5.1.18" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="F6QDM2"/>
    <dbReference type="FunCoup" id="F6QDM2">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000020777"/>
    <dbReference type="Ensembl" id="ENSCINT00000020777.3">
        <property type="protein sequence ID" value="ENSCINP00000020777.3"/>
        <property type="gene ID" value="ENSCING00000021658.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000169396"/>
    <dbReference type="HOGENOM" id="CLU_039475_4_1_1"/>
    <dbReference type="InParanoid" id="F6QDM2"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
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        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
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    <dbReference type="InterPro" id="IPR003080">
        <property type="entry name" value="GST_alpha"/>
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        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050213">
        <property type="entry name" value="GST_superfamily"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11571">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11571:SF230">
        <property type="entry name" value="GLUTATHIONE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14497">
        <property type="entry name" value="GST_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01266">
        <property type="entry name" value="GSTRNSFRASEA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST C-terminal" evidence="4">
        <location>
            <begin position="4"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000020777.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="139" mass="16143" checksum="7FE26DDA9B757636" modified="2012-04-18" version="2">MGENDTERALVDMYTEGIMDMSNVGLAYPFKPDKAQFWSENKEKLGTKYFSVYENILQTTNNGHLVGNKQTMADVLLFESFVHYLHIDKDVLNNYPNLQKFMASQKEIPWVQKFLQPGSNRKPFPDEKYVAMVAEIFFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
            <fullName evidence="6">Transcription initiation factor TFIID subunit 9B-like</fullName>
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    </protein>
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        <name evidence="6" type="primary">LOC100177176</name>
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    <organism evidence="6 7">
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        <name type="common">Transparent sea squirt</name>
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            <taxon>Ciona</taxon>
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    <reference evidence="7" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
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                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
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                <person name="Rokhsar D.S."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAF9 family.</text>
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    <dbReference type="STRING" id="7719.ENSCINP00000025224"/>
    <dbReference type="Ensembl" id="ENSCINT00000025470.2">
        <property type="protein sequence ID" value="ENSCINP00000025224.2"/>
        <property type="gene ID" value="ENSCING00000013825.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000169542"/>
    <dbReference type="HOGENOM" id="CLU_068315_3_1_1"/>
    <dbReference type="InParanoid" id="F6QLU5"/>
    <dbReference type="OMA" id="YEPRVVT"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07979">
        <property type="entry name" value="HFD_TAF9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000018">
        <property type="entry name" value="Transcription initiation factor TFIID subunit 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003162">
        <property type="entry name" value="TFIID-31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051431">
        <property type="entry name" value="TFIID_subunit_9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48068">
        <property type="entry name" value="TAF9 RNA POLYMERASE II, TATA BOX-BINDING PROTEIN (TBP)-ASSOCIATED FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48068:SF4">
        <property type="entry name" value="TATA-BOX BINDING PROTEIN ASSOCIATED FACTOR 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02291">
        <property type="entry name" value="TFIID-31kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000025224.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="181" mass="20654" checksum="F0CCA7E7A062469D" modified="2011-07-27" version="1">MNSVENNPERNKNLPKDAETIIAVLQDMGVTEYEPKVVHQLLEFTYRYISEVLDEAKVYANHAGRSNINVDDTKLAVMSQLDNSFTNPPPRDFLVDVARQKNTIALPLVKNYSAARLPPDRYCLLSVNYRLKGSADKKNVRKNSTKYLTTSTQKHLFANPQVIMPSTNAKRKWHEIGNYDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F6QNP4</accession>
    <name>F6QNP4_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ShKT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">wild type</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001046">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6QNP4"/>
    <dbReference type="Ensembl" id="ENSCINT00000025828.2">
        <property type="protein sequence ID" value="ENSCINP00000025582.2"/>
        <property type="gene ID" value="ENSCING00000014067.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000001027"/>
    <dbReference type="HOGENOM" id="CLU_093014_0_0_1"/>
    <dbReference type="InParanoid" id="F6QNP4"/>
    <dbReference type="OMA" id="NIHYNDV"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003582">
        <property type="entry name" value="ShKT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01549">
        <property type="entry name" value="ShK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51670">
        <property type="entry name" value="SHKT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003340104" description="ShKT domain-containing protein" evidence="2">
        <location>
            <begin position="22"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="ShKT" evidence="3">
        <location>
            <begin position="152"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000025582.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="262" mass="29466" checksum="81A9F4A70A50347A" modified="2012-04-18" version="2">MIVRCRLIAAVLCIVVQVSQAMDLATMVEILRQRTLNNNVNNQTQGPTIQPSDVNQLSEYLLPGGDLEDMIDPEDVPVQVRLVIPPGDLGFSSNELRFGLSVRKSASSRSSEWGEFSCCRQGLNLQSRERMVSDRVMELDKRQCSDNYCESCFDDRPTCRLFKTQIRACHPSSSYRPFMSRHCPETCDMCSATTTTAETTTVNTEETTTEATADPNLVRVNIHYNDVTAPHLYMYSASQSRPRSPWKPVSRSSYVGEVTSFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>F6R107</accession>
    <accession>A0A1W2WM87</accession>
    <name>F6R107_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Lysine-specific histone demethylase 1A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100187100</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">wild type</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the flavin monoamine oxidase family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01003000">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002131150.1">
        <property type="nucleotide sequence ID" value="XM_002131114.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6R107"/>
    <dbReference type="FunCoup" id="F6R107">
        <property type="interactions" value="909"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000013511"/>
    <dbReference type="Ensembl" id="ENSCINT00000013511.3">
        <property type="protein sequence ID" value="ENSCINP00000013511.3"/>
        <property type="gene ID" value="ENSCING00000006580.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100187100"/>
    <dbReference type="KEGG" id="cin:100187100"/>
    <dbReference type="GeneTree" id="ENSGT00940000157193"/>
    <dbReference type="HOGENOM" id="CLU_004498_5_1_1"/>
    <dbReference type="InParanoid" id="F6R107"/>
    <dbReference type="OMA" id="SSRGEMF"/>
    <dbReference type="OrthoDB" id="9982100at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 9"/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
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                <person name="Haga S."/>
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                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
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                <person name="Lee B.I."/>
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                <person name="Mochizuki Y."/>
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                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Hayashi H."/>
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                <person name="Kobayashi K."/>
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                <person name="Manohar C."/>
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                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
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                <person name="Miura S."/>
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                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">wild type</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the Tom70 family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01000470">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6R6J0"/>
    <dbReference type="FunCoup" id="F6R6J0">
        <property type="interactions" value="431"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000018366"/>
    <dbReference type="Ensembl" id="ENSCINT00000018366.3">
        <property type="protein sequence ID" value="ENSCINP00000018366.3"/>
        <property type="gene ID" value="ENSCING00000009049.3"/>
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    <dbReference type="GeneTree" id="ENSGT00940000157095"/>
    <dbReference type="HOGENOM" id="CLU_017516_2_0_1"/>
    <dbReference type="InParanoid" id="F6R6J0"/>
    <dbReference type="OMA" id="QWRGDIE"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 10"/>
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    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030943">
        <property type="term" value="F:mitochondrion targeting sequence binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045039">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46208">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46208:SF1">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13414">
        <property type="entry name" value="TPR_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 10" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="2 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="10">
        <location>
            <begin position="85"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="10">
        <location>
            <begin position="375"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="10">
        <location>
            <begin position="450"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="39"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="44"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="58"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000018366.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="585" mass="65442" checksum="85F6B5C0FA15C4C1" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>F6RBA3</accession>
    <name>F6RBA3_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">wild type</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002965">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6RBA3"/>
    <dbReference type="STRING" id="7719.ENSCINP00000023986"/>
    <dbReference type="Ensembl" id="ENSCINT00000024232.2">
        <property type="protein sequence ID" value="ENSCINP00000023986.2"/>
        <property type="gene ID" value="ENSCING00000012975.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159154"/>
    <dbReference type="HOGENOM" id="CLU_1325980_0_0_1"/>
    <dbReference type="InParanoid" id="F6RBA3"/>
    <dbReference type="OMA" id="PHIVACH"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050538">
        <property type="entry name" value="MAP_kinase_kinase_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48016">
        <property type="entry name" value="MAP KINASE KINASE KINASE SSK2-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48016:SF56">
        <property type="entry name" value="MAPKK KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="65"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="94"/>
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        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000023986.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="207" mass="23499" checksum="22961296DC0E3330" modified="2012-04-18" version="2">MQCSCQMQIEEEEDKLFAHTLAVSYIQDALPMVPHLSYTEEEPDIVRVQDESTDIQKEYKENNQWCKGAQIGLGAFSACYQARDMFTGTLMAVKQVNHVRCSAVEERQVLAVITEEISLMRRLSHPNIVRLHGITKEGPLYNIFIEWCAGGSVSTLLSHYGAFNEAVIMNYTLQLLRGLSYIHEQFLIHRDIKGANLLIDSTGQRFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>F6RKR7</accession>
    <name>F6RKR7_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">L-2-hydroxyglutarate dehydrogenase, mitochondrial</fullName>
            <ecNumber evidence="7">1.1.99.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>(S)-2-hydroxyglutarate + A = 2-oxoglutarate + AH2</text>
            <dbReference type="Rhea" id="RHEA:21252"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:16782"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="EC" id="1.1.99.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the L2HGDH family.</text>
    </comment>
    <dbReference type="EC" id="1.1.99.2" evidence="7"/>
    <dbReference type="AlphaFoldDB" id="F6RKR7"/>
    <dbReference type="FunCoup" id="F6RKR7">
        <property type="interactions" value="268"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000013150"/>
    <dbReference type="Ensembl" id="ENSCINT00000013150.3">
        <property type="protein sequence ID" value="ENSCINP00000013150.3"/>
        <property type="gene ID" value="ENSCING00000006381.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00490000043421"/>
    <dbReference type="HOGENOM" id="CLU_024775_0_0_1"/>
    <dbReference type="InParanoid" id="F6RKR7"/>
    <dbReference type="OMA" id="GVHFTRM"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047545">
        <property type="term" value="F:(S)-2-hydroxyglutarate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008726">
        <property type="entry name" value="PRK11728.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43104">
        <property type="entry name" value="L-2-HYDROXYGLUTARATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43104:SF2">
        <property type="entry name" value="L-2-HYDROXYGLUTARATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="9">
        <location>
            <begin position="2"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00036066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00041137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000013150.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="407" mass="45089" checksum="3C8C56C6626DC7AC" modified="2012-04-18" version="2">YDVTIVGGGIVGLATARELLLRHPGVKYAVVEKENEVSLHQSGHNSGVIHAGIYYTPGSLKAKLCVEGLELMYKYCDQQNIPYKKVGKLIVAVEEEEIPRLDNIYKRALQNGCTVTMLDKHEIKDVEPHCKGLKAIHSPKTGIVDFAVVARSYGKQFAEGGGTIRTNFEVSNFKKSSKLDSGVLIQGKDPNQQIHSKYVIVCGGLYADRLAAKSGCEILPKIVPFRGDYLVLKESKRDLVRGNIYPVPDPRFPFLGVHYTPRMDGSVWLGPNAVLAFKREGYKFFDFNFKDLSEALLFSGLQKLVFRNFSAGVNELYRVANMNAQVKLLQRFIPELKKEDVVKGPSGVRAQALDDKGNLVDDFVFDSGKGELSTNMLHVRNAPSPAATSSLAIAKMIADEVETKYQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>F6RTT6</accession>
    <name>F6RTT6_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01001421">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6RTT6"/>
    <dbReference type="STRING" id="7719.ENSCINP00000028771"/>
    <dbReference type="Ensembl" id="ENSCINT00000029017.2">
        <property type="protein sequence ID" value="ENSCINP00000028771.2"/>
        <property type="gene ID" value="ENSCING00000016701.2"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_638655_0_0_1"/>
    <dbReference type="InParanoid" id="F6RTT6"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015630">
        <property type="term" value="C:microtubule cytoskeleton"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000028771.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="430" mass="50145" checksum="476257E7D1A40653" modified="2012-04-18" version="2">MQRESQSHASNQHNLDLQAHKLSDENQNLLVELKKCNKKIEDLERQSLELASCKSEVLEMKEENNELEIKLKRVGSSFDRHKATSKEKLRSVERDLEESRALMHQQRKEAAENYQELKRAMNRNEEQLKREIAESEEAFNEQKLESDKLIQRLEENLSKSKTKYSQVKEELTEVNSKLFSKLTECENMKEELETKGAVVIMTKQKLQESERVVEQKQVKIEELENKVGCVELEKENAQRQLQIMSGKSDSALAQKENETGNLRIQLNTLQNSLQAEKRKHESMAKVATEVQAKNTALEKQLCDQISKHRVEVESMVESQRVEMLKMKHDAEFATQSLTIKTKSLHKSIRQVTSVLHKLSHENTQLRREVETFPLLLQEGIKRAGKQISEALCEVNESSKDLVRRYRKEMKLRKKYHNELVELKGNIRVFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>F6RYV9</accession>
    <name>F6RYV9_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Mitochondrial carrier protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">wild type</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01000243">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6RYV9"/>
    <dbReference type="FunCoup" id="F6RYV9">
        <property type="interactions" value="198"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000003249"/>
    <dbReference type="Ensembl" id="ENSCINT00000003249.3">
        <property type="protein sequence ID" value="ENSCINP00000003249.3"/>
        <property type="gene ID" value="ENSCING00000001621.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000168859"/>
    <dbReference type="HOGENOM" id="CLU_015166_6_0_1"/>
    <dbReference type="InParanoid" id="F6RYV9"/>
    <dbReference type="OMA" id="WVMYEQM"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015218">
        <property type="term" value="F:pyrimidine nucleotide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990519">
        <property type="term" value="P:pyrimidine nucleotide import into mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049562">
        <property type="entry name" value="SLC25A33/36-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45829">
        <property type="entry name" value="MITOCHONDRIAL CARRIER PROTEIN RIM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45829:SF4">
        <property type="entry name" value="MITOCHONDRIAL CARRIER PROTEIN RIM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="97"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="138"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="194"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="1"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="136"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="246"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000003249.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="331" mass="36264" checksum="0DD0E63D724074E5" modified="2012-04-18" version="2">HFIAGGVAGCAATIVTCPIDVIKTRQQSSSGIATTQAKLSFPSSTPKGTNTRLMTVFARGSLRNTHHTGVVQPALSIQKSSILQHCRYILKVEGGKAFFKGLGIGLVGSVPSRALYFWTYNATKIGLVADFGLKANSMPTSIIAASAGGLVATTATCPLWVLKTKQQLHRRLNNSSLSCFECAKQVWRSEGWKGFYRGLTASYAGIVETVIYFTIYEHLKSWYMKRHNMDVSGAHSSMSSWWELPGLMVISSASKCVATSIVYPHEVARTRLREEPVQQRYSGFWQTLRDVAKTEGRIGLYGGLPAQLVRQVPNMAILMGVYETVVYLGQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>F6S0C7</accession>
    <name>F6S0C7_CIOIN</name>
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        <recommendedName>
            <fullName evidence="1">VWFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="F6S0C7"/>
    <dbReference type="Ensembl" id="ENSCINT00000022629.1">
        <property type="protein sequence ID" value="ENSCINP00000022383.1"/>
        <property type="gene ID" value="ENSCING00000011801.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000012348"/>
    <dbReference type="HOGENOM" id="CLU_784241_0_0_1"/>
    <dbReference type="InParanoid" id="F6S0C7"/>
    <dbReference type="OMA" id="LMERAYN"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032770">
        <property type="entry name" value="DUF4537"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14343:SF5">
        <property type="entry name" value="DUF4537 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14343">
        <property type="entry name" value="VWFA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15057">
        <property type="entry name" value="DUF4537"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13768">
        <property type="entry name" value="VWA_3"/>
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    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VWFA" evidence="1">
        <location>
            <begin position="14"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000022383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="379" mass="42396" checksum="7CA29C535038731E" modified="2011-07-27" version="1">MLINNIFGEIPEQSITFVVDTSGSMYDVLSVVKDHITEVLMERAYNRLGTTFNIIEFSSDITQWADRMVECTPITANAATRWLRGIESQTGSDTLTALHMAFDEPRCQAVYLVTDGMPDQNPEEIIHRITKASYGRPVHCIAVSTGVGDLPLSSTTFLENLAIETRGSFHVVQVGRLGDVSKVNSIYNSVYTPIEGSKKCSVETSLTHNPYTNLLSTLPAPTPPFVPFYRYNPPLHTEVFKEALERTPVASIVVRGTRILARRDEDGYYYGGSVAQEITGDRTKFLVEFDRTKKGRDLRMQETYLHDIIAWNDALRHSVVAEDHVLAPWEEGGKRYGPGKVISGTERRHTIGDYKDSNVVVAFWNGKTVTVEKGVSVWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>F6S0L5</accession>
    <name>F6S0L5_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cytoplasmic FMR1-interacting protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Ciona intestinalis</name>
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            <taxon>Phlebobranchia</taxon>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
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    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
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                <person name="Inaba K."/>
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                <person name="Kobayashi K."/>
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                <person name="Manohar C."/>
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                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
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                <person name="Miura S."/>
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                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
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                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">wild type</strain>
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    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="11" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <reaction evidence="13">
            <text>a very-long-chain acyl-CoA + malonyl-CoA + H(+) = a very-long-chain 3-oxoacyl-CoA + CO2 + CoA</text>
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            <dbReference type="ChEBI" id="CHEBI:57384"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000018836.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="279" mass="32843" checksum="2DE705FE158DEED4" modified="2012-04-18" version="2">KMAALAIQAIEAYNETMYRPDPRSLAYPLIESHAFNVCSTLLYLFIVIFAGPRYMKNRKAFDLRKAIVVYNLTMVVVSAWMVYEFLAAGWATGYSLTCQKVDYSTSPKGLRMLRVCYVYWLSKHVEFLDTYFFIARKKTQQITFLHVFHHTIMAYTWWYGVKFAAGGLGTFHAPLNSFVHVIMYFYYGMAALGPTYRKYIWWKKYVTAIQLIQFVIIFTHIMNILLFQNCEYPPILKYIVLAYCCSFFVLFTNFWIQNYSKKSTKNLPSDKEQQRVKAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>F6S5U6</accession>
    <name>F6S5U6_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BACK domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">wild type</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01001796">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6S5U6"/>
    <dbReference type="STRING" id="7719.ENSCINP00000023304"/>
    <dbReference type="Ensembl" id="ENSCINT00000023550.2">
        <property type="protein sequence ID" value="ENSCINP00000023304.2"/>
        <property type="gene ID" value="ENSCING00000012504.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156265"/>
    <dbReference type="HOGENOM" id="CLU_1296947_0_0_1"/>
    <dbReference type="InParanoid" id="F6S5U6"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632:SF3">
        <property type="entry name" value="KELCH-LIKE PROTEIN 32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632">
        <property type="entry name" value="LD33804P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BACK" evidence="3">
        <location>
            <begin position="79"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000023304.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="222" mass="25149" checksum="2A2A146D36233E88" modified="2012-04-18" version="2">MFRVGDRSYMEATEDSVKLFGVDQDTLQVIVDYIYTGKVQFTPENINSIMKAAGWICPESELLKQGSLYLIERVTTQNCFQTEIFAESYALTQLKEACSDFILKNLSQTKSTLSFTKLSSEYLRSVLSEDRLFAVSEKELFDAVLDWGVSHSAVEVLDLLSKVIRTSLMEISELEASVEAVMNFGIPSHPTVDFFKDSLRFAHLAPMEERILETGQQHRNCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>F6SDF5</accession>
    <name>F6SDF5_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">wild type</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Synaptic cell membrane</location>
            <topology evidence="13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the ligand-gated ion channel (TC 1.A.9) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002481">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6SDF5"/>
    <dbReference type="STRING" id="7719.ENSCINP00000017876"/>
    <dbReference type="Ensembl" id="ENSCINT00000017876.3">
        <property type="protein sequence ID" value="ENSCINP00000017876.3"/>
        <property type="gene ID" value="ENSCING00000008784.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166039"/>
    <dbReference type="HOGENOM" id="CLU_018074_1_4_1"/>
    <dbReference type="InParanoid" id="F6SDF5"/>
    <dbReference type="OMA" id="THEMPRV"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005892">
        <property type="term" value="C:acetylcholine-gated channel complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015464">
        <property type="term" value="F:acetylcholine receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022848">
        <property type="term" value="F:acetylcholine-gated monoatomic cation-selective channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005231">
        <property type="term" value="F:excitatory extracellular ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904315">
        <property type="term" value="F:transmitter-gated monoatomic ion channel activity involved in regulation of postsynaptic membrane potential"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0095500">
        <property type="term" value="P:acetylcholine receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051899">
        <property type="term" value="P:membrane depolarization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034220">
        <property type="term" value="P:monoatomic ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18997">
        <property type="entry name" value="LGIC_ECD_nAChR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19064">
        <property type="entry name" value="LGIC_TM_nAChR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000099">
        <property type="entry name" value="Cholinergic receptor, nicotinic, epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000075">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="1"/>
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    </evidence>
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        <source>
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    <sequence length="357" mass="41453" checksum="A1FE60EDA275488E" modified="2012-04-18" version="2">MTTTVLIVILFCLLDLSASLKNSTFFSKEAELVSILLDGYSARVRPRDKNGKTKVNVGITLSQIMSLSEKSEVMMSSMYMTYTWVDPRFSWNESKYDGILVTRISVDRIWVPNIIIKNSKERNLKESLRVNALVFHNGSVQWLPPAIYKTSCTMDVTLFPFDWQNCSMVFRSADHDQSEMIFEFPITKTLLYFFLSVYIKNGEWVLYQKPAYVVEKKSDEQFQEVWFTVILCRKPQFYVLNMLLPCVMISILSCFVFHLPVVAGEKIGLSISLLLGDTIFVFLFAQRMPSTALTVPHVATYLLFSMILLFLSIFCCVVVYNFHFRSNVTHEMPRVWKWLFFDKFAPLLGLQQPHRKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F6SGW2</accession>
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        <name evidence="4" type="primary">LOC100101819</name>
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        <name type="scientific">Ciona intestinalis</name>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">wild type</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <location>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
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        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>F6SMX2</accession>
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            <fullName evidence="1">Uncharacterized protein</fullName>
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        <name type="scientific">Ciona intestinalis</name>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
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                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
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                <person name="Meinertzhagen I.A."/>
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                <person name="Putnam N."/>
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                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
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                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
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                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
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                <person name="Richardson P."/>
                <person name="Lucas S."/>
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                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
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            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1">wild type</strain>
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        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
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    </evidence>
    <sequence length="206" mass="22910" checksum="BF77367F83D92053" modified="2011-07-27" version="1">AFETSFNPCLKLRTPEQASEISLDLVYSNVAVLPGATDKSNQSLLIIFADEIVWQKCNVGSIALTRYLLYLTSRVHGVCLLIDERGAYDTSASAILEALHIYQNNSPECIKKVLILSDASSLLQPIVKNLFNVECEIVSSDSDLEEFIDSKNLLVQLGGELQFSQAEWIQNRLVVDSFLHFCENVRHNFARHGLSMTSQSLPDSAT</sequence>
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    <accession>F6SNR0</accession>
    <name>F6SNR0_CIOIN</name>
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            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">wild type</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001386">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6SNR0"/>
    <dbReference type="STRING" id="7719.ENSCINP00000022067"/>
    <dbReference type="Ensembl" id="ENSCINT00000022313.2">
        <property type="protein sequence ID" value="ENSCINP00000022067.2"/>
        <property type="gene ID" value="ENSCING00000011582.2"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
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        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
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        <property type="entry name" value="Kinase-like_dom_sf"/>
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        <property type="entry name" value="Prot_kinase_dom"/>
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        <property type="entry name" value="Protein_kinase_ATP_BS"/>
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        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
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    <dbReference type="PANTHER" id="PTHR44329:SF293">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
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        <location>
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        <ligand>
            <name>ATP</name>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
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    </evidence>
    <sequence length="236" mass="27059" checksum="35CBDB1A728B0F43" modified="2012-04-18" version="2">MLEPLQRSSTDILNMLREETESIRSEQGESVRFHALNNICYKMESFRRDLENLINAEKSEILPEEFGLGSKKNVKLGGGSFGVVYKSNYKGTSVAVKVMNDTKDENNRDKELNYLKVLRHDNIIGFRGCGMYQSKRFIVLEYIKENLYLFTKNYSSDDGEGLSAFMAWGISKQLANVMNYLHSRPMTHGDLKPDNILVDRSTVNPRIKLCDFGLACRKDEYTADKSEGHVRYKKKG</sequence>
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    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">wild type</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="EAAA01000348">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6T8C2"/>
    <dbReference type="Ensembl" id="ENSCINT00000015482.3">
        <property type="protein sequence ID" value="ENSCINP00000015482.3"/>
        <property type="gene ID" value="ENSCING00000007556.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165957"/>
    <dbReference type="HOGENOM" id="CLU_006855_2_1_1"/>
    <dbReference type="InParanoid" id="F6T8C2"/>
    <dbReference type="OMA" id="IPELCER"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000175">
        <property type="entry name" value="Na/ntran_symport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037272">
        <property type="entry name" value="SNS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616">
        <property type="entry name" value="SODIUM/CHLORIDE DEPENDENT TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616:SF309">
        <property type="entry name" value="TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00209">
        <property type="entry name" value="SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00176">
        <property type="entry name" value="NANEUSMPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161070">
        <property type="entry name" value="SNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50267">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0915">Sodium</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="104"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="151"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="184"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="230"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="286"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="317"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="258"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR600175-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000015482.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="355" mass="40057" checksum="9FA91FBF33D48595" modified="2012-04-18" version="2">MIIIETQRRVLKFWNGKIVQVSFTDNEWNTESCRVGVNITNRTVFLQDSLPQVQFWRNYVLRLSNGNPYNENFNWQMLLCSLASFITIYGCIAKGVKTSGKVVYFTATFPYVILCVLFVRGATLEGASNGMRKLFVPDFERLLSPQVWVEAASQALYTVGVCYSGLTAFGSFNRRRNNFYKQAIFVIPAGCLTSIFMGLITFSFLGHIAHVNKVDLTNVISSGPGLVFQVYPYGLSLLPIPQLWSVLFFFMFYLICIDSAFGEFEGLMVVIGDAFPISVSNTKNRLLTGAALTASLYFSCFMFLNQKSLYIFELVNSYGGAGICLLWLALWESISVGWFWNADKFAIDFKYMTGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>F6TMW1</accession>
    <name>F6TMW1_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Dual specificity protein phosphatase 23</fullName>
            <ecNumber evidence="5">3.1.3.16</ecNumber>
            <ecNumber evidence="4">3.1.3.48</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Low molecular mass dual specificity phosphatase 3</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">wild type</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Protein phosphatase that mediates dephosphorylation of proteins phosphorylated on Tyr and Ser/Thr residues. In vitro, it can dephosphorylate p44-ERK1 (MAPK3) but not p54 SAPK-beta (MAPK10) in vitro. Able to enhance activation of JNK and p38 (MAPK14).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 20">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="5"/>
    <dbReference type="EC" id="3.1.3.48" evidence="4"/>
    <dbReference type="EMBL" id="EAAA01002337">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6TMW1"/>
    <dbReference type="STRING" id="7719.ENSCINP00000005828"/>
    <dbReference type="Ensembl" id="ENSCINT00000005828.3">
        <property type="protein sequence ID" value="ENSCINP00000005828.3"/>
        <property type="gene ID" value="ENSCING00000002863.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161329"/>
    <dbReference type="HOGENOM" id="CLU_047330_4_2_1"/>
    <dbReference type="InParanoid" id="F6TMW1"/>
    <dbReference type="OMA" id="PAHYQYL"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14504">
        <property type="entry name" value="DUSP23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000063">
        <property type="entry name" value="Dual specificity phosphatase 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050561">
        <property type="entry name" value="PTP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057023">
        <property type="entry name" value="PTP-SAK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23339">
        <property type="entry name" value="TYROSINE SPECIFIC PROTEIN PHOSPHATASE AND DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22784">
        <property type="entry name" value="PTP-SAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="16">
        <location>
            <begin position="31"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="17">
        <location>
            <begin position="102"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00053915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00068789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00081937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000005828.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="183" mass="20713" checksum="09CAB54F1C7C2943" modified="2012-04-18" version="2">YLQYHSSHSHNKNTSNNQAASRRNVPEPPSNFTWVDPGWLAACASPTCPSHHKFLFECGIRHVISLMKQRPTNAMESVQLPKLNRLKIRIDDYCAPTLNQIRQFIRIVEDANDRKEAVAVHCANGKGISGTMLACYLVKMKRISAVDALKEIRRMRPGSVESAEQEKAVEQFYQSLLSLCQNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>F6TQ55</accession>
    <name>F6TQ55_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nuclear speckle splicing regulatory protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Coiled-coil domain-containing protein 55</fullName>
        </alternativeName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">wild type</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NSRP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001833">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6TQ55"/>
    <dbReference type="STRING" id="7719.ENSCINP00000017538"/>
    <dbReference type="Ensembl" id="ENSCINT00000017538.3">
        <property type="protein sequence ID" value="ENSCINP00000017538.3"/>
        <property type="gene ID" value="ENSCING00000015091.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154049"/>
    <dbReference type="HOGENOM" id="CLU_040467_1_1_1"/>
    <dbReference type="InParanoid" id="F6TQ55"/>
    <dbReference type="OMA" id="QSRDHEN"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000381">
        <property type="term" value="P:regulation of alternative mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042816">
        <property type="entry name" value="Nsrp1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018612">
        <property type="entry name" value="NSRP1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31938">
        <property type="entry name" value="NUCLEAR SPECKLE SPLICING REGULATORY PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31938:SF4">
        <property type="entry name" value="NUCLEAR SPECKLE SPLICING REGULATORY PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09745">
        <property type="entry name" value="NSRP1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nuclear speckle splicing regulatory protein 1 N-terminal" evidence="7">
        <location>
            <begin position="67"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="216"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="160"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="235"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="259"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="352"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00030718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF09745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000017538.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="415" mass="48977" checksum="11DCE2C79DD92336" modified="2012-04-18" version="2">MSFTKQYGLIIRKDKPTLKKVLNKSSILKPRLAAFGGDSSEEEDGHKDVNAQIHKEAMKKIVKKQTKIEIQKAMEQDATVYEYDSIYDDMKQKNAIKNTPAAVVSADKQKAPKYIGQLMKAAESRAREKQRVEERKIERERKMEGDQFSDKPAFVTSAYKKRMEERQQEEEEERRMAAIEDAMDVKKQRDLSGFYKHLLNRQVGETRFVFYYFRSVKKEPPSEPEVPATTSDPPPDNDEDADTDISFSDSDEEEGSSGEEQKKRGDRERRKNDEKNRSRDEKRDSRDLDYRERSKDQKRTSKKDKEDSVEKERRSSDQKESRSRKEESRHRNRREDSREEERTSSSRRKHRDPTPDNKRREGERSSINQHEEGKSKRDNERGREEKSNSESNKRSRDDKKDNKFKITDEEKSKTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>F6TZG1</accession>
    <name>F6TZG1_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Sulfatase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">wild type</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sulfatase family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002914">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6TZG1"/>
    <dbReference type="STRING" id="7719.ENSCINP00000013992"/>
    <dbReference type="Ensembl" id="ENSCINT00000013992.3">
        <property type="protein sequence ID" value="ENSCINP00000013992.3"/>
        <property type="gene ID" value="ENSCING00000006821.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166538"/>
    <dbReference type="HOGENOM" id="CLU_006332_10_1_1"/>
    <dbReference type="InParanoid" id="F6TZG1"/>
    <dbReference type="OMA" id="HEHCVFI"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008484">
        <property type="term" value="F:sulfuric ester hydrolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd16029">
        <property type="entry name" value="4-S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047115">
        <property type="entry name" value="ARSB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024607">
        <property type="entry name" value="Sulfatase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000917">
        <property type="entry name" value="Sulfatase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10342">
        <property type="entry name" value="ARYLSULFATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10342:SF274">
        <property type="entry name" value="ARYLSULFATASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00884">
        <property type="entry name" value="Sulfatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00149">
        <property type="entry name" value="SULFATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sulfatase N-terminal" evidence="8">
        <location>
            <begin position="1"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="473"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000013992.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="492" mass="55810" checksum="0FAFC922B8668834" modified="2012-04-18" version="2">PNIVFILADDYGFNDIGYHAVEHHSDMKTPFLDSLAMAGVRLENYYIQPICSPSRSVLMSGRYQIHTGLQHYVISPQQRNGLPLDNIILPEQLHKCGYDTHMVGKWHLGFYKDEYLPWKRGFNSYFGYLTGGEDYYTKWRCDGKLCGYDMTSEKGPTNATYGQYSANLFANKANEAIDKHDKTKPLFLYVAFQSVHSPMEVPESYAKPFDYIKNHNRKMYGGMVAAMDEAVKNITEHLQAAGLWDNTILVFSADNGGQTLSGGNNWPLRGRKLTLWEGGIKGVGFVHGKILNVPNPNYIVNNEMIHISDWFPTIMEATQCPYVEGTQKLDGVNQWDTIFGKTVSARKEILLNIDPLFTPLFYKETPKQNIQMGFDTSVHAGIRYGEWKLLTGLQGDDRWIEPPEWKNKAEEEQQEDTEHPGFQKFATKSVQLYNIVNDPYEQVEVSDLYPSVVNDLLSKLAAYNATAVPVNYPPSDPTSDPSLHGGFWGPWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>F6U7K8</accession>
    <name>F6U7K8_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">receptor protein-tyrosine kinase</fullName>
            <ecNumber evidence="2">2.7.10.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">wild type</strain>
        </source>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.10.1" evidence="2"/>
    <dbReference type="EMBL" id="EAAA01001150">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6U7K8"/>
    <dbReference type="STRING" id="7719.ENSCINP00000026874"/>
    <dbReference type="Ensembl" id="ENSCINT00000027120.2">
        <property type="protein sequence ID" value="ENSCINP00000026874.2"/>
        <property type="gene ID" value="ENSCING00000000360.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160232"/>
    <dbReference type="HOGENOM" id="CLU_015796_1_0_1"/>
    <dbReference type="InParanoid" id="F6U7K8"/>
    <dbReference type="OMA" id="RQTHISK"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004714">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006909">
        <property type="term" value="P:phagocytosis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000089">
        <property type="entry name" value="Tyrosine-protein kinase receptor TYRO3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000662">
        <property type="entry name" value="Tyrosine-protein kinase receptor UFO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000509">
        <property type="entry name" value="Tyrosine-protein kinase receptor UFO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050122">
        <property type="entry name" value="RTK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416:SF564">
        <property type="entry name" value="MACROPHAGE-STIMULATING PROTEIN RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000615">
        <property type="entry name" value="TyrPK_CSF1-R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="18" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-0675">Receptor</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="12" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="390"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="21">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="22">
        <location>
            <begin position="187"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="20">
        <location>
            <begin position="478"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="16">
        <location>
            <position position="625"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="629"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="630"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="643"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR000615-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR000615-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR000615-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000026874.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="757" mass="83748" checksum="70514D0E53BFB0F2" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>F6UB97</accession>
    <name>F6UB97_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">wild type</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>glutathione = L-cysteinylglycine + 5-oxo-L-proline</text>
            <dbReference type="Rhea" id="RHEA:47724"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:58402"/>
            <dbReference type="ChEBI" id="CHEBI:61694"/>
            <dbReference type="EC" id="4.3.2.7"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the gamma-glutamylcyclotransferase family. ChaC subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001863">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6UB97"/>
    <dbReference type="STRING" id="7719.ENSCINP00000024985"/>
    <dbReference type="Ensembl" id="ENSCINT00000025231.2">
        <property type="protein sequence ID" value="ENSCINP00000024985.2"/>
        <property type="gene ID" value="ENSCING00000013657.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000003855"/>
    <dbReference type="HOGENOM" id="CLU_070703_2_2_1"/>
    <dbReference type="InParanoid" id="F6UB97"/>
    <dbReference type="OMA" id="WIFGYGE"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061928">
        <property type="term" value="F:glutathione specific gamma-glutamylcyclotransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006751">
        <property type="term" value="P:glutathione catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06661">
        <property type="entry name" value="GGCT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.490.10">
        <property type="entry name" value="Gamma-glutamyl cyclotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006840">
        <property type="entry name" value="ChaC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013024">
        <property type="entry name" value="GGCT-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12192">
        <property type="entry name" value="CATION TRANSPORT PROTEIN CHAC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12192:SF26">
        <property type="entry name" value="GLUTATHIONE-SPECIFIC GAMMA-GLUTAMYLCYCLOTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04752">
        <property type="entry name" value="ChaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00048073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000024985.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="213" mass="24094" checksum="FCC015255144AC14" modified="2012-04-18" version="2">MNSIDLDITSENQVRNVEKQLTSNEISEWLHQGSGFKWSCPETQSLLVFGYGSLVWLPKFAFHNREVGYVHGFVRRFWQGNISHRGVPGSPGRVVTLVPDDKGVTWGVTFHLKGKKQIEAALRHLVTREMINGGYEMRTAKFYGHRTYDILTFVAMPDNAHYLGPAPVETVAATIVNSRGRSGHNLEYIFNLIDTLRREAPQGVDNHLTSLEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>F6UFE4</accession>
    <name>F6UFE4_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Gamma-tubulin complex component</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">wild type</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Cytoskeleton</location>
            <location evidence="4">Microtubule organizing center</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TUBGCP family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01000168">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6UFE4"/>
    <dbReference type="STRING" id="7719.ENSCINP00000005211"/>
    <dbReference type="Ensembl" id="ENSCINT00000005211.3">
        <property type="protein sequence ID" value="ENSCINP00000005211.3"/>
        <property type="gene ID" value="ENSCING00000013711.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157810"/>
    <dbReference type="HOGENOM" id="CLU_415959_0_0_1"/>
    <dbReference type="InParanoid" id="F6UFE4"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005815">
        <property type="term" value="C:microtubule organizing center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043015">
        <property type="term" value="F:gamma-tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007020">
        <property type="term" value="P:microtubule nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007259">
        <property type="entry name" value="GCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041470">
        <property type="entry name" value="GCP_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19302">
        <property type="entry name" value="GAMMA TUBULIN COMPLEX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19302:SF70">
        <property type="entry name" value="GAMMA-TUBULIN COMPLEX COMPONENT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17681">
        <property type="entry name" value="GCP_N_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="2 4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gamma tubulin complex component protein N-terminal" evidence="7">
        <location>
            <begin position="107"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="639"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="453"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
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                <person name="Mochizuki Y."/>
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                <person name="Yagi K."/>
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                <person name="Zhang C."/>
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                <person name="Doggett N."/>
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                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
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                <person name="Huang W."/>
                <person name="Kawashima T."/>
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                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
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                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
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                <person name="Suzuki M.M."/>
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    <reference evidence="12" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">wild type</strain>
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    <reference evidence="12" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01001911">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002125733.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6V0S1"/>
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        <property type="protein sequence ID" value="ENSCINP00000024935.2"/>
        <property type="gene ID" value="ENSCING00000013626.2"/>
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    <dbReference type="KEGG" id="cin:100175793"/>
    <dbReference type="GeneTree" id="ENSGT00560000077514"/>
    <dbReference type="HOGENOM" id="CLU_090201_2_0_1"/>
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        <property type="component" value="Chromosome 4"/>
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    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
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        <property type="project" value="InterPro"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Vaccinia Virus protein VP39"/>
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    </dbReference>
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        <property type="entry name" value="Methyltransf_11"/>
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        <property type="entry name" value="SAM-dependent_MTases_sf"/>
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        <property type="entry name" value="METHYLTRANSFERASE-LIKE PROTEIN 27"/>
        <property type="match status" value="1"/>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="1">
        <location>
            <begin position="71"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000024935.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="234" mass="26109" checksum="62514E06A73EA952" modified="2011-07-27" version="1">MANKPLNEAYGRIINVSPADDSPAATQKYYNENWETWEIDMKSMTYTAADVAAQKAASLVSREQMEHFKLLDLGAGTGLSGVALRRAGFPGKISALDGSPGMLKLAMEKSIYSDATEHLLTSSNPLPYPDQAFDMVVNVGGFTIHHMEPDCLEDVLRVLKPLSYLVVTVRLHTGEHKVRFNGELDRLEAAGLMKKYSRETFLQYKWNEVSDDKFKVNYADQYGDLLVLQKPGNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>F6VD75</accession>
    <name>F6VD75_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Crossover junction endonuclease MUS81</fullName>
            <ecNumber evidence="13">3.1.22.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Interacts with EME1 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, D-loops, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication fork intermediates. May be required in meiosis for the repair of meiosis-specific double strand breaks subsequent to single-end invasion (SEI).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="13">Interacts with EME1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the XPF family.</text>
    </comment>
    <dbReference type="EC" id="3.1.22.-" evidence="13"/>
    <dbReference type="AlphaFoldDB" id="F6VD75"/>
    <dbReference type="FunCoup" id="F6VD75">
        <property type="interactions" value="11"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000003159"/>
    <dbReference type="Ensembl" id="ENSCINT00000003159.3">
        <property type="protein sequence ID" value="ENSCINP00000003159.3"/>
        <property type="gene ID" value="ENSCING00000001582.3"/>
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    <dbReference type="GeneTree" id="ENSGT00390000005498"/>
    <dbReference type="HOGENOM" id="CLU_014329_1_1_1"/>
    <dbReference type="InParanoid" id="F6VD75"/>
    <dbReference type="OMA" id="ELGDAMW"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048476">
        <property type="term" value="C:Holliday junction resolvase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048257">
        <property type="term" value="F:3'-flap endonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008821">
        <property type="term" value="F:crossover junction DNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006308">
        <property type="term" value="P:DNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0031573">
        <property type="term" value="P:mitotic intra-S DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0031297">
        <property type="term" value="P:replication fork processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0000712">
        <property type="term" value="P:resolution of meiotic recombination intermediates"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="XPF_nuclease_Mus81"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Crossover junction endonuclease MUS81"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Putative Crossover junction endonuclease MUS81"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.670">
        <property type="entry name" value="Crossover junction endonuclease EME1, DNA-binding domain"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="1.10.150.110">
        <property type="entry name" value="DNA polymerase beta, N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR027421">
        <property type="entry name" value="DNA_pol_lamdba_lyase_dom_sf"/>
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        <property type="entry name" value="EME1/EME2_C"/>
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    <dbReference type="InterPro" id="IPR006166">
        <property type="entry name" value="ERCC4_domain"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR033309">
        <property type="entry name" value="Mus81"/>
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    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
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    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047417">
        <property type="entry name" value="WHD_MUS81"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047416">
        <property type="entry name" value="XPF_nuclease_Mus81"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13451">
        <property type="entry name" value="CLASS II CROSSOVER JUNCTION ENDONUCLEASE MUS81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13451:SF0">
        <property type="entry name" value="CROSSOVER JUNCTION ENDONUCLEASE MUS81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21292">
        <property type="entry name" value="EME1-MUS81_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02732">
        <property type="entry name" value="ERCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14716">
        <property type="entry name" value="HHH_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21136">
        <property type="entry name" value="WHD_MUS81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00891">
        <property type="entry name" value="ERCC4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47802">
        <property type="entry name" value="DNA polymerase beta, N-terminal domain-like"/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
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                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
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                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
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                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
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                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
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                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
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                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
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                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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            <authorList>
                <consortium name="Ensembl"/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satake M."/>
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                <person name="Azumi K."/>
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                <person name="Haga S."/>
                <person name="Hayashi H."/>
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                <person name="Imai K.S."/>
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                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
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                <person name="Makabe K.W."/>
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                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
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                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
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                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
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                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
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                <person name="Richardson P."/>
                <person name="Lucas S."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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            <authorList>
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            <begin position="125"/>
            <end position="145"/>
        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    </evidence>
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    <accession>F6W6I5</accession>
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            <fullName evidence="2">calcium/calmodulin-dependent protein kinase</fullName>
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    <reference evidence="17" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
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                <person name="Chapman J."/>
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                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
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                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">wild type</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.17"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.17"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.</text>
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    <dbReference type="EC" id="2.7.11.17" evidence="2"/>
    <dbReference type="EMBL" id="EAAA01001499">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="F6W6I5"/>
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        <property type="interactions" value="28"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000004087"/>
    <dbReference type="Ensembl" id="ENSCINT00000004087.3">
        <property type="protein sequence ID" value="ENSCINP00000004087.3"/>
        <property type="gene ID" value="ENSCING00000002022.3"/>
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    <dbReference type="GeneTree" id="ENSGT00940000159769"/>
    <dbReference type="HOGENOM" id="CLU_000288_71_0_1"/>
    <dbReference type="InParanoid" id="F6W6I5"/>
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    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 2"/>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="C:postsynaptic density"/>
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        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0004683">
        <property type="term" value="F:calcium/calmodulin-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0048168">
        <property type="term" value="P:regulation of neuronal synaptic plasticity"/>
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        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:1903076">
        <property type="term" value="P:regulation of protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="CDD" id="cd14086">
        <property type="entry name" value="STKc_CaMKII"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Calcium/calmodulin-dependent protein kinase type II subunit delta"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Peripheral plasma membrane protein CASK isoform B"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013543">
        <property type="entry name" value="Ca/CaM-dep_prot_kinase-assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
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    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
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    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08332">
        <property type="entry name" value="CaMKII_AD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Pkinase"/>
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    </dbReference>
    <dbReference type="SMART" id="SM00220">
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
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    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 12 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 13" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="1"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="301"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005354"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
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            <dbReference type="ARBA" id="ARBA00012434"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022527"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047307"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00047430"/>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="PROSITE" id="PS50011"/>
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    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    </evidence>
    <sequence length="477" mass="53962" checksum="EE94025C12233B72" modified="2012-04-18" version="2">ILAEELGKGAFSVVKRCTHRSSKQDYAAKIINTKKLSSRDLQKLDREARICRLLKHTNIVRLHASIPEEGLHFLVFDLVTGGELFEDIVAREYYSEADASHCMQQVLEAVYHCHKSGVIHRDLKPENLLLASKEKGALVKLADFGLAIEVNDDQPAWYGFAGTPGYLSPEVLRKEPYGKPVDLWACGVILYILLVGYPPFWDDDQNRLYAQIKDGAYDFPSPEWDTVTKEAKDLINQMLSTKTSDRITASDALRNPWICQRDRVASHIHRQDTVDCLKTFNARRKLKGAIITTMLATRNFSRSSMSRNKESNKEPQAQSTVVRANSAVLKPTGENGLDLTAEEIKAKKLEVFHATEQLMQSINSRNFAAYSRFCSNDFTCFDPLSMGNLMHGNDFQKYYFETAPKSEHLRKSSILNPHIVLLGGDVAYIAYICLTQYIDGSGKPVSVQTEETRIWNKVEGSWRNSHLHRSESVTARK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>F6W6K0</accession>
    <name>F6W6K0_CIOIN</name>
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        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
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        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">wild type</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
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            <dbReference type="ChEBI" id="CHEBI:15378"/>
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        <property type="gene ID" value="ENSCING00000002018.3"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:innate immune response"/>
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    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
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    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
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    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
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    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
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    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
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    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
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    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
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    <dbReference type="PANTHER" id="PTHR18934:SF237">
        <property type="entry name" value="ATP-DEPENDENT DNA_RNA HELICASE DHX36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
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        <property type="entry name" value="DEAD"/>
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    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
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    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
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    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
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    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="DEAH_ATP_HELICASE"/>
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        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="8">
        <location>
            <begin position="29"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="9">
        <location>
            <begin position="228"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="534" mass="60270" checksum="B9986D18ADA6D2B9" modified="2025-10-08" version="3"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>F6WAG8</accession>
    <accession>A0A1W2W644</accession>
    <name>F6WAG8_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Enoyl-CoA hydratase, mitochondrial</fullName>
            <ecNumber evidence="5">4.2.1.17</ecNumber>
            <ecNumber evidence="4">5.3.3.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="24">Enoyl-CoA hydratase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">Short-chain enoyl-CoA hydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="28" type="primary">LOC100186995</name>
    </gene>
    <organism evidence="28 29">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="29" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="28">wild type</strain>
        </source>
    </reference>
    <reference evidence="28" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="28" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>(3E)-hexenoyl-CoA = (2E)-hexenoyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:45736"/>
            <dbReference type="ChEBI" id="CHEBI:62077"/>
            <dbReference type="ChEBI" id="CHEBI:84790"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:45737"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>(3S)-3-hydroxybutanoyl-CoA = (2E)-butenoyl-CoA + H2O</text>
            <dbReference type="Rhea" id="RHEA:26558"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57316"/>
            <dbReference type="ChEBI" id="CHEBI:57332"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="20">
            <dbReference type="Rhea" id="RHEA:26560"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>(3S)-hydroxydecanoyl-CoA = (2E)-decenoyl-CoA + H2O</text>
            <dbReference type="Rhea" id="RHEA:31191"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:61406"/>
            <dbReference type="ChEBI" id="CHEBI:62616"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="13">
            <dbReference type="Rhea" id="RHEA:31193"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>(3S)-hydroxyhexanoyl-CoA = (2E)-hexenoyl-CoA + H2O</text>
            <dbReference type="Rhea" id="RHEA:30547"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:62075"/>
            <dbReference type="ChEBI" id="CHEBI:62077"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="18">
            <dbReference type="Rhea" id="RHEA:30549"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>2-methylpropenoyl-CoA + H2O = (S)-3-hydroxyisobutanoyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:31175"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:62500"/>
            <dbReference type="ChEBI" id="CHEBI:62611"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:31176"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>3-hydroxybutanoyl-CoA = (2E)-butenoyl-CoA + H2O</text>
            <dbReference type="Rhea" id="RHEA:45584"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57332"/>
            <dbReference type="ChEBI" id="CHEBI:78611"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="19">
            <dbReference type="Rhea" id="RHEA:45586"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>3-hydroxyisovaleryl-CoA = 3-methylbut-2-enoyl-CoA + H2O</text>
            <dbReference type="Rhea" id="RHEA:31079"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57344"/>
            <dbReference type="ChEBI" id="CHEBI:62555"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="26">
            <dbReference type="Rhea" id="RHEA:31081"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>3-hydroxypropanoyl-CoA = acryloyl-CoA + H2O</text>
            <dbReference type="Rhea" id="RHEA:26518"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57367"/>
            <dbReference type="ChEBI" id="CHEBI:58528"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="16">
            <dbReference type="Rhea" id="RHEA:26520"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a (3E)-enoyl-CoA = a 4-saturated (2E)-enoyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:45228"/>
            <dbReference type="ChEBI" id="CHEBI:58521"/>
            <dbReference type="ChEBI" id="CHEBI:85097"/>
            <dbReference type="EC" id="5.3.3.8"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:45229"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a (3S)-3-hydroxyacyl-CoA = a (2E)-enoyl-CoA + H2O</text>
            <dbReference type="Rhea" id="RHEA:16105"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57318"/>
            <dbReference type="ChEBI" id="CHEBI:58856"/>
            <dbReference type="EC" id="4.2.1.17"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="14">
            <dbReference type="Rhea" id="RHEA:16107"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="21">Homohexamer; dimer of trimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 27">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.17" evidence="5"/>
    <dbReference type="EC" id="5.3.3.8" evidence="4"/>
    <dbReference type="EMBL" id="EAAA01001663">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002125020.1">
        <property type="nucleotide sequence ID" value="XM_002124984.5"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_026689376.1">
        <property type="nucleotide sequence ID" value="XM_026833575.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_026689377.1">
        <property type="nucleotide sequence ID" value="XM_026833576.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6WAG8"/>
    <dbReference type="FunCoup" id="F6WAG8">
        <property type="interactions" value="275"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000002248"/>
    <dbReference type="Ensembl" id="ENSCINT00000002248.3">
        <property type="protein sequence ID" value="ENSCINP00000002248.3"/>
        <property type="gene ID" value="ENSCING00000005478.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100186995"/>
    <dbReference type="KEGG" id="cin:100186995"/>
    <dbReference type="GeneTree" id="ENSGT00940000157609"/>
    <dbReference type="HOGENOM" id="CLU_009834_7_6_1"/>
    <dbReference type="InParanoid" id="F6WAG8"/>
    <dbReference type="OMA" id="FCDARED"/>
    <dbReference type="OrthoDB" id="2018133at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004165">
        <property type="term" value="F:delta(3)-delta(2)-enoyl-CoA isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
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    <dbReference type="GO" id="GO:0004300">
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    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Enoyl-CoA hydratase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.12.10:FF:000001">
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        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.12.10">
        <property type="entry name" value="Lyase 2-enoyl-coa Hydratase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR018376">
        <property type="entry name" value="Enoyl-CoA_hyd/isom_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014748">
        <property type="entry name" value="Enoyl-CoA_hydra_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11941:SF54">
        <property type="entry name" value="ENOYL-COA HYDRATASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11941">
        <property type="entry name" value="ENOYL-COA HYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00166">
        <property type="entry name" value="ENOYL_COA_HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="11" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-0456">Lyase</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005254"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00035760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00035854"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00035949"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00036137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00036353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00036370"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00036643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00036765"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00038629"/>
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            <dbReference type="ARBA" id="ARBA00051535"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00052675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="RuleBase" id="RU003707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000002248.3"/>
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    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="291" mass="31512" checksum="7E3E08BE536CB320" modified="2012-04-18" version="2">MAGYLAKASTNLLRKNISTYTALLRFSSSDTKQYENIIVEKRAENGCVGLIQLNRPKALNSLCDALMREVNEALDTFEQDESVGCVVLTGNERAFAAGADIKEMQNNTFVGCLKGNFLGHWSEIAQKKKPIIAAVNGFALGGGCELAMMCDIIYAGDNAQFGQPEILLGTIPGAGGTQRLPKFVGKSLAMEMVLTGNRFDAQTALTAGLVSKVVSKELLVDEAIKTAEKISKNSQLVVALAKEAVNIAYETTLAEGLHFEKKLFHATFATEDRKEGMTAFVEKRKANFTNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>F6WR24</accession>
    <name>F6WR24_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="F6WR24"/>
    <dbReference type="Ensembl" id="ENSCINT00000028548.2">
        <property type="protein sequence ID" value="ENSCINP00000028302.2"/>
        <property type="gene ID" value="ENSCING00000016286.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000065389"/>
    <dbReference type="HOGENOM" id="CLU_892909_0_0_1"/>
    <dbReference type="InParanoid" id="F6WR24"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003345535" evidence="1">
        <location>
            <begin position="27"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000028302.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="312" mass="35680" checksum="DBEF8EAD896D438E" modified="2012-04-18" version="2">MNRHGLTAAWMCVMIINNFNLDCVQAFGIRLCRNVTVRETKAIDFSYKVSVNISDELVFIVTASWDLWNPKNVSLDGFLYLFLKVNNQIMDDKVSLPTTDHCNPGANHFWVTTSNTSVCSQVEGCNSTYQCLCDVNCQLTRSTPPCRSLEYWMSQGWRSVPGHYRSVSFIVEYFSFYDFDVTPYYRQYIGNNSGQHLNRLYRNLPPLNNCAQLVQQQSGKSFAESQDICCNLTQPLARNITFSQPILDYVKDTINITVTWLPPITRDLLSFFSVRVEFKDPSEWISPGPRTVGSPQNISFNYSNVYLGDCDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>F6WS34</accession>
    <name>F6WS34_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">wild type</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002776">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6WS34"/>
    <dbReference type="STRING" id="7719.ENSCINP00000004302"/>
    <dbReference type="Ensembl" id="ENSCINT00000004302.3">
        <property type="protein sequence ID" value="ENSCINP00000004302.3"/>
        <property type="gene ID" value="ENSCING00000002105.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165312"/>
    <dbReference type="HOGENOM" id="CLU_000288_63_23_1"/>
    <dbReference type="InParanoid" id="F6WS34"/>
    <dbReference type="OMA" id="IMEFCER"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000097">
        <property type="entry name" value="Probable serine/threonine-protein kinase nek1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051997">
        <property type="entry name" value="STK_NEK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44535">
        <property type="entry name" value="PROTEIN CBG16200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44535:SF5">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="41"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000004302.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="204" mass="22932" checksum="846BD44765E393B1" modified="2011-07-27" version="1">MLGMDTILDVGDGKQTVGEFYFKSDKLRSFNNGSYQTIDLFEKIRMVGKGAFGTAVLYKKKDDNSLVVLKEINMSELNSQERQMAMNETRVLAMLNHPNIICYYDTFEDNGVLMIEMEYADDGTLAQFLAKSESAIEEKKIFSMFSQIVSGLAHMHDQKVLHRDLKTANIFLTKEGVVKIGDFGVSKVISSRIAANTVLGWYIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>F6X7V3</accession>
    <accession>A0A1W2W5X9</accession>
    <name>F6X7V3_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized LOC100184555</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100184555</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">wild type</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="EAAA01002715">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002124868.1">
        <property type="nucleotide sequence ID" value="XM_002124832.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6X7V3"/>
    <dbReference type="Ensembl" id="ENSCINT00000015376.3">
        <property type="protein sequence ID" value="ENSCINP00000015376.3"/>
        <property type="gene ID" value="ENSCING00000007501.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100184555"/>
    <dbReference type="KEGG" id="cin:100184555"/>
    <dbReference type="GeneTree" id="ENSGT00390000004250"/>
    <dbReference type="HOGENOM" id="CLU_1524597_0_0_1"/>
    <dbReference type="InParanoid" id="F6X7V3"/>
    <dbReference type="OMA" id="AFRINTA"/>
    <dbReference type="OrthoDB" id="6072491at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019577">
        <property type="entry name" value="SPARC/Testican_Ca-bd-dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10591">
        <property type="entry name" value="SPARC_Ca_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5014090383" evidence="6">
        <location>
            <begin position="20"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="7">
        <location>
            <begin position="145"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000015376.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="182" mass="20745" checksum="73F3730E87140FAB" modified="2012-04-18" version="2">MKLFLTYFTLVFFICLGDTFPLGPETRSLRMLEGESRPTHHHHHNHHHSDEDVNRLRRLDQIIGRIEDAAISRESDDEPIPDVDASDLSSVADVVSLVDNVVDTPDVKLNQLAFQIIALPHKFSRYDLNRDGYVTQLELAESTGTLLEDCADPFERADKDGDGLLTKEELHQAPWVFNERAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F6XFL7</accession>
    <name>F6XFL7_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">wild type</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CD36 family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002245">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="EAAA01002246">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6XFL7"/>
    <dbReference type="Ensembl" id="ENSCINT00000004025.2">
        <property type="protein sequence ID" value="ENSCINP00000004025.2"/>
        <property type="gene ID" value="ENSCING00000001996.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153372"/>
    <dbReference type="HOGENOM" id="CLU_1389778_0_0_1"/>
    <dbReference type="InParanoid" id="F6XFL7"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002159">
        <property type="entry name" value="CD36_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11923:SF12">
        <property type="entry name" value="PLATELET GLYCOPROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11923">
        <property type="entry name" value="SCAVENGER RECEPTOR CLASS B TYPE-1 SR-B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01130">
        <property type="entry name" value="CD36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="102"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000004025.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="196" mass="22078" checksum="6A69ADB4A10631C0" modified="2011-07-27" version="1">MISSPHFLYGDGFYLNMTTGIEPPNVLEHEAALVYEPFTGAAIKADKRLQINILLKPTPKINVFSKINEELVFPVVWINESATVGKSDADLLYGLLVLPAEVVYILQIILCILGSLLFLGPLFYFVVLHYSNVDEKDTRKPNGAVPEMKSYNVYENENVFDDVKEYKIGEPEHTNTRVTSTTSTTELIENQKGMKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>F6XH38</accession>
    <name>F6XH38_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Glutamate--cysteine ligase</fullName>
            <ecNumber evidence="9">6.3.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Gamma-ECS</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Gamma-glutamylcysteine synthetase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">wild type</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>L-cysteine + L-glutamate + ATP = gamma-L-glutamyl-L-cysteine + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13285"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58173"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterodimer of a catalytic heavy chain and a regulatory light chain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the glutamate--cysteine ligase type 3 family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.2" evidence="9"/>
    <dbReference type="EMBL" id="EAAA01001506">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6XH38"/>
    <dbReference type="FunCoup" id="F6XH38">
        <property type="interactions" value="31"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000007643"/>
    <dbReference type="Ensembl" id="ENSCINT00000007643.3">
        <property type="protein sequence ID" value="ENSCINP00000007643.3"/>
        <property type="gene ID" value="ENSCING00000003707.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000011908"/>
    <dbReference type="HOGENOM" id="CLU_010467_0_0_1"/>
    <dbReference type="InParanoid" id="F6XH38"/>
    <dbReference type="OMA" id="IAHMFIR"/>
    <dbReference type="UniPathway" id="UPA00142">
        <property type="reaction ID" value="UER00209"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017109">
        <property type="term" value="C:glutamate-cysteine ligase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004357">
        <property type="term" value="F:glutamate-cysteine ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006750">
        <property type="term" value="P:glutathione biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.590.50:FF:000007">
        <property type="entry name" value="Glutamate--cysteine ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.960:FF:000001">
        <property type="entry name" value="Glutamate--cysteine ligase catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.590.50:FF:000002">
        <property type="entry name" value="Glutamate--cysteine ligase catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.960">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.590.50">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004308">
        <property type="entry name" value="GCS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11164">
        <property type="entry name" value="GLUTAMATE CYSTEINE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11164:SF0">
        <property type="entry name" value="GLUTAMATE--CYSTEINE LIGASE CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03074">
        <property type="entry name" value="GCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 9" id="KW-0317">Glutathione biosynthesis</keyword>
    <keyword evidence="4 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">wild type</strain>
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    <reference evidence="4" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
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        <property type="entry name" value="Glutaredoxin"/>
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        <property type="entry name" value="Glutathione_S-Trfase"/>
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        <property type="entry name" value="Glutathione_S-Trfase_N"/>
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        <property type="entry name" value="Thioredoxin-like_sf"/>
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        <property type="entry name" value="GLUTATHIONE S TRANSFERASE D10, ISOFORM A-RELATED"/>
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        <property type="entry name" value="GLUTATHIONE S TRANSFERASE D10, ISOFORM A-RELATED"/>
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            <end position="80"/>
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        <location>
            <begin position="87"/>
            <end position="214"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        <source>
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        <source>
            <dbReference type="PROSITE" id="PS50405"/>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000022773.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="223" mass="25039" checksum="DCD01FEE50B48929" modified="2012-04-18" version="2">ITLHSLPASPPGRCVVTTLHVLGLEFEEKHINSIAKEHKKPAFLAMNPRGCFPVLTDGDFIIAESFAIAMYLCNKYEKGENRLYPLQPEVRGKVDQLLFVGSVLYDAALQYLNLLGVIYDGGVMAPEKLPGVYHALGLCEAFLQGDFMVGDHITLAVLDIFMSCGLYLLDLVDFNDYGAYPKTVAWLKRVRNWSYFKTANDEGMKELENIYRPLLAKNTQNRS</sequence>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <dbReference type="InParanoid" id="F6Y0I3"/>
    <dbReference type="OMA" id="TAWRFGF"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
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    <dbReference type="InterPro" id="IPR004302">
        <property type="entry name" value="Cellulose/chitin-bd_N"/>
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    <dbReference type="PANTHER" id="PTHR21113">
        <property type="entry name" value="AGAP001705-PA"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR21113:SF4">
        <property type="entry name" value="CHITIN-BINDING TYPE-4 DOMAIN-CONTAINING PROTEIN"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
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        </location>
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        <location>
            <begin position="18"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000001876.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="189" mass="21240" checksum="9768B4B4A49D3F3D" modified="2012-04-18" version="2">MLLKGLIALLLIHHAQPHGRMMEPPNRSSLWRFWDTDPAIIPYKSIVVPNYGDNELFCGGFQHQVNLGGKCGICGDAYDGVRDNEGGGKYAKGIIVRQYAPGSIFNVNIELTAHHMGYFEFRLCKLDNPLVAATQDCLNQNLLLTEDGHTKYYPPAVKSTIMKYDIKLRLPADLTCTQCVLQWRYHTGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>F6Y321</accession>
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    <protein>
        <recommendedName>
            <fullName evidence="8">t-SNARE coiled-coil homology domain-containing protein</fullName>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
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            <taxon>Phlebobranchia</taxon>
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            <taxon>Ciona</taxon>
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    <reference evidence="10" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
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                <person name="Di Gregorio A."/>
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                <person name="Goodstein D.M."/>
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                <person name="Hastings K.E."/>
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                <person name="Hotta K."/>
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                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
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                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
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                <person name="Medina M."/>
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                <person name="Mount S."/>
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                <person name="Tassy O."/>
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                <person name="Tokuoka M."/>
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                <person name="Yoshizaki F."/>
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                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
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                <person name="Richardson P."/>
                <person name="Lucas S."/>
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                <person name="Levine M."/>
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            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the syntaxin family.</text>
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    <dbReference type="FunCoup" id="F6Y321">
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        <property type="protein sequence ID" value="ENSCINP00000011472.3"/>
        <property type="gene ID" value="ENSCING00000005538.3"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="F:SNARE binding"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
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                <person name="Sano M."/>
                <person name="Sasaki A."/>
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                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
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                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
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                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
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                <person name="Miura S."/>
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                <person name="Nomoto H."/>
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                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
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                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="136"/>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="180"/>
            <end position="368"/>
        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="13"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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            <begin position="88"/>
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        <location>
            <begin position="120"/>
            <end position="135"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="183"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="198"/>
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            <begin position="359"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000026120.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="368" mass="42153" checksum="7CF86EEF4141C5AB" modified="2012-04-18" version="2">MSFKRNDKKQEFSDNFNNNHNSSENSPSLVETDEMTTDEEAIVWQESRQVVDAYLKRSLSQNAKSSLTIMKPEGYHKLKKDGRLPFYQRQQSRPEQGSLVRTSNQEDKRHRSSVPAKNITPKTTLPSRSSMNELSSHGRRIVDPLYEVSRSVTDINDDIDMPIEDGIMKKLIDSEHSLEYVPSSDSLPSTSGEGNTITRRKSFTKATRKSVISMIENQKRRERQNSSYQQQQQQQQVHFTSKTPDPIRKTLSVKSRKSSLGELHYHEFSKEEQDGLKRETGGNEERKVVEKKISFRDRMKRIMGGPSNKKKLRYKNSFKQRQTSVEENKKSADKSSSGSNQGFDFGSSSSKQPSRERTPSPSKMKGSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
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        <name type="common">Transparent sea squirt</name>
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            <taxon>Metazoa</taxon>
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            <taxon>Phlebobranchia</taxon>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    <proteinExistence type="predicted"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000027875.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="263" mass="29394" checksum="F780C9F8E1AE85AF" modified="2012-04-18" version="2">MGSMPHPTINTKVNILLKMEETIFQEDPDLLELIRLASVGKSSIDRLSGKFLTPDGKKSKSTNFDPLALPVVVLTSDETDQPRPNKRLCFSDSKNLETKKRFRDEAINASHQLNNNDSSNSEKLLTKKLKRRKLINADSSSLAQSVVPIDDSAENVPSDLKSAEKSPTHHSPTPLVEDSKLKDKKLTENVENLNISSVNTVHIVSIPNVNEEKLRETTDSAIPPSGRLLRKRIRKTLPPLKLRQTPLGLPKQPRVTKDEFTIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>F6ZEA6</accession>
    <name>F6ZEA6_CIOIN</name>
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        <recommendedName>
            <fullName evidence="12">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
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            <taxon>Tunicata</taxon>
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    <reference evidence="14" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">wild type</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="F6ZEA6"/>
    <dbReference type="Ensembl" id="ENSCINT00000012804.2">
        <property type="protein sequence ID" value="ENSCINP00000012804.2"/>
        <property type="gene ID" value="ENSCING00000006204.2"/>
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        <property type="component" value="Chromosome 12"/>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    </dbReference>
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        <property type="term" value="P:neuropeptide signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
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        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24238">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24238:SF74">
        <property type="entry name" value="PROKINETICIN RECEPTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="52"/>
            <end position="76"/>
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    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="96"/>
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        </location>
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    <feature type="transmembrane region" description="Helical" evidence="11">
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            <begin position="162"/>
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    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="220"/>
            <end position="240"/>
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    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="355"/>
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    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="389"/>
            <end position="410"/>
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    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="32"/>
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        <location>
            <begin position="291"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="301"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
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    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000012804.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="444" mass="50480" checksum="02D57A6EC0F86F63" modified="2011-07-27" version="1">MMNLTFNVDKCSSTKHWEFLVLLPFGFLSIAFNLTLVVAATFNRRRLQRQSYVYACVTSTLLGNLLYVILHTWIILDNYLTKIEESGGDYTVTWTILQVSVASMLFVMCGNIGSLLFVVLDSTYFVGRSVNRKASEFAKGFISDIESVRSRRWLLSAKRRRAVILIALSWLLPICLVISAATTWNCSNQCVYCRGGSPLVPPCANHTHCSTVWPPLTESYIGVNFFLWILEVFILGYLIVCGIRSFQQVMAPPTRTSSVMPVVKEENSVAESDIAAQEEYTLSDVHNAMTSLGDSDEEIKEEEKKNDEKEEKNDVKKSMKRDRHENGGKEVEMAVNDVDPRIVRSRPSFRSHSRLTFMIVISLTFVVCSFPTFVVFLVDTFANISNRKVARLTTMVMMYIYTFICPCLLLKYLPNLKSALILLFTNCSVTRIPKRRSKRETTAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F6ZLG3</accession>
    <name>F6ZLG3_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01002517">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6ZLG3"/>
    <dbReference type="FunCoup" id="F6ZLG3">
        <property type="interactions" value="31"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSCINT00000022681.2">
        <property type="protein sequence ID" value="ENSCINP00000022435.2"/>
        <property type="gene ID" value="ENSCING00000011834.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000003990"/>
    <dbReference type="HOGENOM" id="CLU_1547002_0_0_1"/>
    <dbReference type="InParanoid" id="F6ZLG3"/>
    <dbReference type="OMA" id="EEEFIMV"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001726">
        <property type="term" value="C:ruffle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051895">
        <property type="term" value="P:negative regulation of focal adhesion assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900025">
        <property type="term" value="P:negative regulation of substrate adhesion-dependent cell spreading"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.360.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019517">
        <property type="entry name" value="Integrin-bd_ICAP-1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32055">
        <property type="entry name" value="INTEGRIN BETA-1-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32055:SF1">
        <property type="entry name" value="INTEGRIN BETA-1-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10480">
        <property type="entry name" value="ICAP-1_inte_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="11"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="53"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000022435.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="173" mass="18455" checksum="314EFED65AEB2425" modified="2011-07-27" version="1">MPIFHRQTKPSSSGDGSDRSVSVSSSRSKSFDSSEDLSHSSSVSTPEFEMKQSGTGSFSSTRSSIPDSPLLSSPASEYSVKVLGVLDNIEGQGDGPLEVINAIDIAQSEEKIPFFAIGEDVFVSISMNGFQVTDNNQRFLGQKITRCFSHVLASVLSSSPMVTDESGEDDESS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>F7A4U1</accession>
    <accession>A0A1W5BCX8</accession>
    <name>F7A4U1_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Fibroblast growth factor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">wild type</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the heparin-binding growth factors family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001176">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F7A4U1"/>
    <dbReference type="STRING" id="7719.ENSCINP00000010439"/>
    <dbReference type="Ensembl" id="ENSCINT00000010439.3">
        <property type="protein sequence ID" value="ENSCINP00000010439.3"/>
        <property type="gene ID" value="ENSCING00000005062.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165881"/>
    <dbReference type="HOGENOM" id="CLU_432728_0_0_1"/>
    <dbReference type="InParanoid" id="F7A4U1"/>
    <dbReference type="OMA" id="DSRISNW"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005104">
        <property type="term" value="F:fibroblast growth factor receptor binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008543">
        <property type="term" value="P:fibroblast growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022008">
        <property type="term" value="P:neurogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008284">
        <property type="term" value="P:positive regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043410">
        <property type="term" value="P:positive regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030334">
        <property type="term" value="P:regulation of cell migration"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00058">
        <property type="entry name" value="beta-trefoil_FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002209">
        <property type="entry name" value="Fibroblast_GF_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008996">
        <property type="entry name" value="IL1/FGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056378">
        <property type="entry name" value="Let-756-like_FGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11486">
        <property type="entry name" value="FIBROBLAST GROWTH FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00167">
        <property type="entry name" value="FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00262">
        <property type="entry name" value="IL1HBGF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00442">
        <property type="entry name" value="FGF"/>
        <property type="match status" value="1"/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
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                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
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                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
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                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
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                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
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                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
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                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
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                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
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                <person name="Zeller R.W."/>
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                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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        <text evidence="4">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.</text>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
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                <person name="Kobayashi K."/>
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                <person name="Makabe K.W."/>
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                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
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                <person name="Nishizaka S."/>
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                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
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                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
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                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">wild type</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001467">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F7ACP0"/>
    <dbReference type="SMR" id="F7ACP0"/>
    <dbReference type="FunCoup" id="F7ACP0">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSCINT00000022114.2">
        <property type="protein sequence ID" value="ENSCINP00000021868.2"/>
        <property type="gene ID" value="ENSCING00000011448.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01060000248575"/>
    <dbReference type="HOGENOM" id="CLU_924251_0_0_1"/>
    <dbReference type="InParanoid" id="F7ACP0"/>
    <dbReference type="OMA" id="PLLDAHW"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051360">
        <property type="entry name" value="Neuronal_Pentraxin_Related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001759">
        <property type="entry name" value="PTX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19277:SF161">
        <property type="entry name" value="LAMININ G DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19277">
        <property type="entry name" value="PENTRAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00354">
        <property type="entry name" value="Pentaxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00895">
        <property type="entry name" value="PENTAXIN"/>
    </dbReference>
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        <property type="entry name" value="PTX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS51828">
        <property type="entry name" value="PTX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pentraxin (PTX)" evidence="8">
        <location>
            <begin position="97"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="48"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000021868.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="301" mass="33642" checksum="0463282F20F0E662" modified="2012-04-18" version="2">MFFVSCLHCLVSGDCQSNTNQVQICAPISSFTPIPGNSGDFFSQLNGRAAGSRGKAGPKGEKGSRGPPGLEADNECNCNQELERLRNEIRELKYGCPHIMFNFPRLNQITDYVRYRPYFPRLSSATICMWVRTSDFTHNCLLSYASSDSRNNEMVIMLEAATRIDFYLYNSRQKIFRIPSMMNQWTHLCFWFSTATSQLGMYVNGTSLGSFRYPGSNIAGGGTLIIAQEQDGVNAGGLERSQAFSGQISSPIIWQRVLNSSEILDVTQRCICPRDHAITMTMDNVELHGATQYEMPDCLVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>F7AH68</accession>
    <accession>A0A1W2WHC8</accession>
    <name>F7AH68_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">N-terminal E2 ubiquitin-conjugating enzyme</fullName>
            <ecNumber evidence="2">2.3.2.23</ecNumber>
            <ecNumber evidence="6">2.3.2.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100177184</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.</text>
            <dbReference type="EC" id="2.3.2.23"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-N-terminal-amino acid = [E1 ubiquitin-activating enzyme]-L-cysteine + N-terminal-ubiquitinyl-[acceptor protein].</text>
            <dbReference type="EC" id="2.3.2.25"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="2"/>
    <dbReference type="EC" id="2.3.2.25" evidence="6"/>
    <dbReference type="RefSeq" id="XP_002129103.1">
        <property type="nucleotide sequence ID" value="XM_002129067.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F7AH68"/>
    <dbReference type="FunCoup" id="F7AH68">
        <property type="interactions" value="127"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000005658"/>
    <dbReference type="Ensembl" id="ENSCINT00000005658.3">
        <property type="protein sequence ID" value="ENSCINP00000005658.3"/>
        <property type="gene ID" value="ENSCING00000002766.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100177184"/>
    <dbReference type="KEGG" id="cin:100177184"/>
    <dbReference type="GeneTree" id="ENSGT00940000156908"/>
    <dbReference type="HOGENOM" id="CLU_030988_15_1_1"/>
    <dbReference type="InParanoid" id="F7AH68"/>
    <dbReference type="OMA" id="HPICASI"/>
    <dbReference type="OrthoDB" id="406833at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006513">
        <property type="term" value="P:protein monoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd23808">
        <property type="entry name" value="UBCc_UBE2W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000022">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="8">
        <location>
            <begin position="5"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00042168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000005658.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="152" mass="17314" checksum="D58F30E77D35FB88" modified="2011-07-27" version="1">MSAHFVQKRIQKEILTMRKSPPPGIRLCEDSLRSAPEFMVELTGATGTLYSEQIFKLLFKFGERYPFESPQVTFVGNCIPVHPHVYSNGHICLSILTEDWSPALSTEAVCLSVISMLSSCTEKKLPPDNAFYIRTCSKNPKDTKWWFHDDQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>F7APJ4</accession>
    <name>F7APJ4_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Nuclear receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">tr2/4</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">wild type</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nuclear hormone receptor family. NR2 subfamily.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="interactions" value="301"/>
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    <dbReference type="STRING" id="7719.ENSCINP00000003290"/>
    <dbReference type="Ensembl" id="ENSCINT00000003290.3">
        <property type="protein sequence ID" value="ENSCINP00000003290.3"/>
        <property type="gene ID" value="ENSCING00000001639.3"/>
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    <dbReference type="GeneID" id="778864"/>
    <dbReference type="KEGG" id="cin:778864"/>
    <dbReference type="CTD" id="778864"/>
    <dbReference type="GeneTree" id="ENSGT00940000168581"/>
    <dbReference type="HOGENOM" id="CLU_397913_0_0_1"/>
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    <dbReference type="OMA" id="NCGELCV"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 8"/>
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    <dbReference type="GO" id="GO:0005634">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:nuclear receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
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        <property type="entry name" value="NR2C1/2-like_LBD"/>
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        <property type="entry name" value="Nucl_hrmn_rcpt_lig-bd"/>
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        <property type="entry name" value="Nuclear_hrmn_rcpt"/>
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        <property type="entry name" value="Znf_hrmn_rcpt"/>
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    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
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    <dbReference type="PANTHER" id="PTHR24083">
        <property type="entry name" value="NUCLEAR HORMONE RECEPTOR"/>
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        <property type="entry name" value="Hormone_recep"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00105">
        <property type="entry name" value="zf-C4"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="STRDHORMONER"/>
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        <property type="entry name" value="STROIDFINGER"/>
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    <dbReference type="SMART" id="SM00430">
        <property type="entry name" value="HOLI"/>
        <property type="match status" value="1"/>
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    <dbReference type="SMART" id="SM00399">
        <property type="entry name" value="ZnF_C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
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        <property type="entry name" value="Nuclear receptor ligand-binding domain"/>
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        <property type="entry name" value="NR_LBD"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS00031">
        <property type="entry name" value="NUCLEAR_REC_DBD_1"/>
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    <dbReference type="PROSITE" id="PS51030">
        <property type="entry name" value="NUCLEAR_REC_DBD_2"/>
        <property type="match status" value="1"/>
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    <keyword evidence="8 11" id="KW-0804">Transcription</keyword>
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    <keyword evidence="5 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 11" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="203"/>
            <end position="278"/>
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    <feature type="domain" description="NR LBD" evidence="14">
        <location>
            <begin position="371"/>
            <end position="630"/>
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    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
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    <feature type="region of interest" description="Disordered" evidence="12">
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            <begin position="78"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="12">
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        <source>
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        <source>
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    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    <evidence type="ECO:0000256" key="4">
        <source>
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    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
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    <evidence type="ECO:0000256" key="6">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
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    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
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        <source>
            <dbReference type="PROSITE" id="PS51030"/>
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            <dbReference type="PROSITE" id="PS51843"/>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000003290.3"/>
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    <evidence type="ECO:0000313" key="16">
        <source>
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</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>F7ATF3</accession>
    <name>F7ATF3_CIOIN</name>
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        <recommendedName>
            <fullName evidence="11">Zinc-finger domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
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            <taxon>Ciona</taxon>
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    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">wild type</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="EAAA01000176">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="F7ATF3"/>
    <dbReference type="STRING" id="7719.ENSCINP00000009323"/>
    <dbReference type="Ensembl" id="ENSCINT00000009323.3">
        <property type="protein sequence ID" value="ENSCINP00000009323.3"/>
        <property type="gene ID" value="ENSCING00000004508.3"/>
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    <dbReference type="HOGENOM" id="CLU_035988_1_0_1"/>
    <dbReference type="InParanoid" id="F7ATF3"/>
    <dbReference type="OMA" id="TRSMGGC"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040221">
        <property type="entry name" value="CDCA7/CDA7L"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018866">
        <property type="entry name" value="Znf-4CXXC_R1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31169">
        <property type="entry name" value="OS05G0300700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31169:SF8">
        <property type="entry name" value="ZINC-FINGER DOMAIN OF MONOAMINE-OXIDASE A REPRESSOR R1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10497">
        <property type="entry name" value="zf-4CXXC_R1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Zinc-finger" evidence="11">
        <location>
            <begin position="177"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="127"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="10"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="130"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF10497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000009323.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="286" mass="32294" checksum="2B259FA5CDB9B984" modified="2012-04-18" version="2">KSSDGGSTLEESDSDSDSDDDHQSIVKHQKAQMQAKKQMLSQLMAELDKDPVFLKMKTSTPEQIKRFQTTKKLVRSPPRRYTQNIRGPPLTRRRSSMVGNVEYPDGLNDDGADTIRSRLTVRFTSMKRKLSNDDPAERTPKRRHVSSLSQPHIIIPVEDVTQEMLDNIAEHSVGKTYDTINGTSCHQCRQKTIDTKSCCRGEDCVGVRGQFCGPCLKNRYGELVENVLLDSEWKCPVCRNICNCSICRNRKGKCATGILIGIARQQGHSNVRAYLENLENETKLYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>F7AVQ6</accession>
    <name>F7AVQ6_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">wild type</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01002046">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F7AVQ6"/>
    <dbReference type="STRING" id="7719.ENSCINP00000023048"/>
    <dbReference type="Ensembl" id="ENSCINT00000023294.2">
        <property type="protein sequence ID" value="ENSCINP00000023048.2"/>
        <property type="gene ID" value="ENSCING00000012312.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000005656"/>
    <dbReference type="HOGENOM" id="CLU_038694_0_0_1"/>
    <dbReference type="InParanoid" id="F7AVQ6"/>
    <dbReference type="OMA" id="LRKHPCK"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051308">
        <property type="term" value="P:male meiosis chromosome separation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007127">
        <property type="term" value="P:meiosis I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033587">
        <property type="entry name" value="M1AP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28642">
        <property type="entry name" value="MEIOSIS 1 ARREST PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28642:SF1">
        <property type="entry name" value="MEIOSIS 1 ARREST PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000023048.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="391" mass="44232" checksum="0473CFD3DE052BFC" modified="2012-04-18" version="2">KKEMTSFVNNVRYPFARQPGQCVIVDLSTTYTQDNIEAVCNGITRFSNLLCLMKNGPRRVPHFSILCALTYPEVLLPFIQLNNESHTRIQGAVEELRRLSRETTNHCGASSVKMLEQALREAVNQFHKFKRNDLFFSKLEVTVMCFRPPQTLLALVKTTLTGIDISDIPRVTLFGMAPTGDYPVPDDITLSNDDADLTGILELVSGEVDSVCVEMFLRRLLSDKSTDREHVHLYFNSGLRIKCDLQERVLDPSEFIFSQHFVLSSNADHIKNVQLKAVKPGESVPITQLQIIQQVPSASVCESVLFGHPYVMYNSRCWRMEWNELETNQNRFITFSQYLIDNDVCVVCKNITQQSNKRGRIQLPTALFLIMAAPNPATLLIKSIASGTLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>F7B4P3</accession>
    <name>F7B4P3_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; phospholipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="14">Phospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="F7B4P3"/>
    <dbReference type="FunCoup" id="F7B4P3">
        <property type="interactions" value="136"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000005007"/>
    <dbReference type="Ensembl" id="ENSCINT00000005007.3">
        <property type="protein sequence ID" value="ENSCINP00000005007.3"/>
        <property type="gene ID" value="ENSCING00000002465.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234574"/>
    <dbReference type="HOGENOM" id="CLU_025017_0_0_1"/>
    <dbReference type="InParanoid" id="F7B4P3"/>
    <dbReference type="OMA" id="MELEFMP"/>
    <dbReference type="UniPathway" id="UPA00085"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047184">
        <property type="term" value="F:1-acylglycerophosphocholine O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042171">
        <property type="term" value="F:lysophosphatidic acid acyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd07991">
        <property type="entry name" value="LPLAT_LPCAT1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045252">
        <property type="entry name" value="LPCAT1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23063:SF52">
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<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
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                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
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                <person name="Hino K."/>
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                <person name="Inaba K."/>
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                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">wild type</strain>
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    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <dbReference type="SAM" id="SignalP"/>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000022663.1"/>
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        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    </evidence>
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<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
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    <reference evidence="16" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
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                <person name="Meinertzhagen I.A."/>
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                <person name="Putnam N."/>
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                <person name="Wang H.G."/>
                <person name="Awazu S."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="15" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="15">wild type</strain>
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    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="15" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <text evidence="1">Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion inner membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
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        <subcellularLocation>
            <location evidence="2">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the complex I NDUFB7 subunit family.</text>
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        <property type="nucleotide sequence ID" value="XM_002123532.5"/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
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                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
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                <person name="Tassy O."/>
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                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
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                <person name="Detter C."/>
                <person name="Doggett N."/>
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                <person name="Lucas S."/>
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                <person name="Levine M."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
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            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">wild type</strain>
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    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Chapman J."/>
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                <person name="De Tomaso A."/>
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                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <property type="entry name" value="Ras GTP-binding protein YPT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057289">
        <property type="entry name" value="Rab1/Ypt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0636">Prenylation</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="175"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000030419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="204" mass="22732" checksum="8A394A108FBE2445" modified="2012-04-18" version="1">MTMNPEYDYLFKLLLIGDSGVGKSCLLLRFADDTYTESYISTIGVDFKIRTIELDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIVVYDVTDQESFNNVKQWLQEIDRYASENVNKLLVGNKCDLTTKKVVDYTTAKEYADTLKIPFLETSAKTANNVEQAFMTMAAEIKNRMGPGTAASPGQSNKVDISSKPVDGKQGGCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>H2XLP3</accession>
    <accession>A0A1W2W7W1</accession>
    <name>H2XLP3_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">26S proteasome non-ATPase regulatory subunit 14</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11 12">26S Proteasome regulatory subunit RPN11</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC100179683</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">wild type</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. The PSMD14 subunit is a metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains within the complex. Plays a role in response to double-strand breaks (DSBs): acts as a regulator of non-homologous end joining (NHEJ) by cleaving 'Lys-63'-linked polyubiquitin, thereby promoting retention of JMJD2A/KDM4A on chromatin and restricting TP53BP1 accumulation. Also involved in homologous recombination repair by promoting RAD51 loading.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the 19S proteasome regulatory particle complex. The 26S proteasome consists of a 20S core particle (CP) and two 19S regulatory subunits (RP). The regulatory particle is made of a lid composed of 9 subunits including PSMD4, a base containing 6 ATPases and few additional components. Within the complex, PSMD4 interacts with subunit PSMD7 through their respective MPN domain. Interacts with TXNL1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the peptidase M67A family. PSMD14 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002788">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002122648.1">
        <property type="nucleotide sequence ID" value="XM_002122612.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XLP3"/>
    <dbReference type="FunCoup" id="H2XLP3">
        <property type="interactions" value="1199"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000030575"/>
    <dbReference type="MEROPS" id="M67.A10"/>
    <dbReference type="Ensembl" id="ENSCINT00000030855.1">
        <property type="protein sequence ID" value="ENSCINP00000030575.1"/>
        <property type="gene ID" value="ENSCING00000020086.1"/>
    </dbReference>
    <dbReference type="GeneID" id="100179683"/>
    <dbReference type="KEGG" id="cin:100179683"/>
    <dbReference type="GeneTree" id="ENSGT00730000111116"/>
    <dbReference type="HOGENOM" id="CLU_052991_0_1_1"/>
    <dbReference type="InParanoid" id="H2XLP3"/>
    <dbReference type="OMA" id="KTGRHEM"/>
    <dbReference type="OrthoDB" id="605656at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008541">
        <property type="term" value="C:proteasome regulatory particle, lid subcomplex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140492">
        <property type="term" value="F:metal-dependent deubiquitinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070628">
        <property type="term" value="F:proteasome binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd08069">
        <property type="entry name" value="MPN_RPN11_CSN5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.10:FF:000001">
        <property type="entry name" value="26S proteasome non-ATPase regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000555">
        <property type="entry name" value="JAMM/MPN+_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050242">
        <property type="entry name" value="JAMM_MPN+_peptidase_M67A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056263">
        <property type="entry name" value="RPN11_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10410">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01398">
        <property type="entry name" value="JAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23594">
        <property type="entry name" value="RPN11_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00232">
        <property type="entry name" value="JAB_MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102712">
        <property type="entry name" value="JAB1/MPN domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="5" id="KW-0647">Proteasome</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="MPN" evidence="13">
        <location>
            <begin position="31"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00060194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00061051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00065289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00068188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00077355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00078982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000030575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="310" mass="34539" checksum="5A18154312FF0AFB" modified="2012-04-18" version="1">MDRLLRLGTGAGGLGQAPSGDAPMVDTAEQVYISSLALLKMLKHGRAGVPMEVMGLMLGEFVDDYTVRVIDVFAMPQSGTGVSVEAVDPVFQAKMLDMLKQTGRPEMVVGWYHSHPGFGCWLSGVDINTQQSFEALSERAVAVVVDPIQSVKGKVVIDAFRLINPNMMVLGQEPRQTTSNLGHLQKPSIQALIHGLNRHYYSIAINHRKNELEQKMLLNLHKRSWIDNLTLEDYNNHCKKNEQTTSDMLQLAKNYKKALEEEEKMTPEQLAIKNVGKQDPKRHLEEKVDSLMTSNITQCLGAVIDTVVFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>H2XLV8</accession>
    <name>H2XLV8_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100177056</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Putnam N."/>
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                <person name="Satake M."/>
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                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
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            <authorList>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Chapman J."/>
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                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
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                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Putnam N."/>
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                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
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                <person name="Zhang C."/>
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                <person name="Richardson P."/>
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                <person name="Levine M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="De Tomaso A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    </evidence>
    <sequence length="307" mass="34642" checksum="D22C6930B3C3CFF6" modified="2012-04-18" version="1">MIEAPYKSTTVCKSRYASTVNCLAEVMSKCQGGFCESLFAYIPGAREGIKMAVEMMRMTAHAEAEKVCSGNFLNDIMGGVQPPFGIGEPVCDNMVLDDMHHWVVLLMKEHMTADTHADICKSTEEFFSKVITAVKTRCNFNAIRQLLPREPEFAEYLIKMSNIIVDLISNDKVPGCQGEVSMASCPALSPNACGKEMKEAWLCDYISWRITLVHKWAPEFLRWSASNTMMEIDMSEMPECRELTVECKNKDQYSKVKDMFGCEIPFCKDGKYSGGTNLSIWKHDHMLWRKAFSVWQAAQAYGGDDMC</sequence>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="7" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">wild type</strain>
        </source>
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    <reference evidence="7" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="7" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="Ensembl" id="ENSCINT00000032004.1">
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        <property type="gene ID" value="ENSCING00000019687.1"/>
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    <dbReference type="InParanoid" id="H2XQG5"/>
    <dbReference type="OMA" id="WRVAYIS"/>
    <dbReference type="Proteomes" id="UP000008144">
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="entry name" value="MFS_Na/Anion_cotransporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
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        <property type="entry name" value="FI19708P1-RELATED"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR11662">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 17"/>
        <property type="match status" value="1"/>
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        <location>
            <begin position="25"/>
            <end position="45"/>
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        <location>
            <begin position="84"/>
            <end position="104"/>
        </location>
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        </location>
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    <feature type="transmembrane region" description="Helical" evidence="5">
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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        </location>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
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    </evidence>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
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                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
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                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
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                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
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                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
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                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">wild type</strain>
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    <reference evidence="1" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000032103.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
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                <person name="Martinez D."/>
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                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
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                <person name="Wiley G.B."/>
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                <person name="Roe B.A."/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Matsumoto J."/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Yamada L."/>
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                <person name="Wiley G.B."/>
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                <person name="Roe B.A."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Ogasawara M."/>
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                <person name="Yamada L."/>
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                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="H2XWL9"/>
    <dbReference type="Ensembl" id="ENSCINT00000032990.1">
        <property type="protein sequence ID" value="ENSCINP00000034053.1"/>
        <property type="gene ID" value="ENSCING00000017953.1"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2966834_0_0_1"/>
    <dbReference type="InParanoid" id="H2XWL9"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000034053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="59" mass="6795" checksum="080EADF209DAAD17" modified="2012-04-18" version="1">TGKWHELYSHHSLIQVLVICCKLSGICIDYTGNQMQYPVLSSNSARFPKKLDMFMKSFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>H2XWY1</accession>
    <name>H2XWY1_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Fanconi-associated nuclease</fullName>
            <ecNumber evidence="17">3.1.4.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Nuclease required for the repair of DNA interstrand cross-links (ICL). Acts as a 5'-3' exonuclease that anchors at a cut end of DNA and cleaves DNA successively at every third nucleotide, allowing to excise an ICL from one strand through flanking incisions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 17">
            <text>Hydrolytically removes 5'-nucleotides successively from the 3'-hydroxy termini of 3'-hydroxy-terminated oligonucleotides.</text>
            <dbReference type="EC" id="3.1.4.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="17">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 17">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 17">Belongs to the FAN1 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.1" evidence="17"/>
    <dbReference type="AlphaFoldDB" id="H2XWY1"/>
    <dbReference type="STRING" id="7719.ENSCINP00000034165"/>
    <dbReference type="Ensembl" id="ENSCINT00000030681.1">
        <property type="protein sequence ID" value="ENSCINP00000034165.1"/>
        <property type="gene ID" value="ENSCING00000025007.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000018637"/>
    <dbReference type="HOGENOM" id="CLU_1621901_0_0_1"/>
    <dbReference type="InParanoid" id="H2XWY1"/>
    <dbReference type="OMA" id="KHEGEAC"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004528">
        <property type="term" value="F:phosphodiesterase I activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036297">
        <property type="term" value="P:interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1350.10:FF:000004">
        <property type="entry name" value="Fanconi-associated nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033315">
        <property type="entry name" value="Fan1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014883">
        <property type="entry name" value="VRR_NUC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15749">
        <property type="entry name" value="FANCONI-ASSOCIATED NUCLEASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15749:SF4">
        <property type="entry name" value="FANCONI-ASSOCIATED NUCLEASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08774">
        <property type="entry name" value="VRR_NUC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00990">
        <property type="entry name" value="VRR_NUC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 17" id="KW-0227">DNA damage</keyword>
    <keyword evidence="14 17" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="11" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="9 17" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12 17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15 17" id="KW-0464">Manganese</keyword>
    <keyword evidence="5 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 17" id="KW-0540">Nuclease</keyword>
    <keyword evidence="16 17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="VRR-NUC" evidence="18">
        <location>
            <begin position="50"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU365033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM00990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000034165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="171" mass="19833" checksum="7012800E23A586E2" modified="2012-04-18" version="1">MWDIIFMPGVKDVFRTRYQSHPLDLYTDHFYEHRKDLIDRRLDDIISWNNDDFIVVDFISEIWNKYNGLACSGLNWERFASLEQVLSLVKCIGGEALSGILGTMVKDFRHTRSGLPDLVVWNDISYKLVEVKGPNDRLSTNQRVWIHKLMELGVDVEVCHVSAAASRKMIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>H2XXX0</accession>
    <name>H2XXX0_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RBR-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.31</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.31"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.31" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="H2XXX0"/>
    <dbReference type="STRING" id="7719.ENSCINP00000034504"/>
    <dbReference type="Ensembl" id="ENSCINT00000034708.1">
        <property type="protein sequence ID" value="ENSCINP00000034504.1"/>
        <property type="gene ID" value="ENSCING00000018714.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158451"/>
    <dbReference type="HOGENOM" id="CLU_1280239_0_0_1"/>
    <dbReference type="InParanoid" id="H2XXX0"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20338">
        <property type="entry name" value="BRcat_RBR_RNF19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16629">
        <property type="entry name" value="RING-HC_RBR_RNF19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000424">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031127">
        <property type="entry name" value="E3_UB_ligase_RBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002867">
        <property type="entry name" value="IBR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11685">
        <property type="entry name" value="RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01485">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00647">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="13">
        <location>
            <begin position="97"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="12">
        <location>
            <begin position="101"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="35"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="50"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000034504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="216" mass="24240" checksum="D8E8F4C2D3339153" modified="2012-04-18" version="1">MSSMSDSQSASSSTKKRRRLFRLPWRRRLRCCGSNNEYRRSNSSSLEDIPSTSKESKVLSQQGVPPAHVFEAAVSVHQTYKSGSQSIGSTSEPDKQNTLECPLCLVAQPVKNFPLLSTCPHRSCSECWVQYLTIEITESRVNLMCPECQERLHPSDMQTILSHEPELVRKWEEFTLRKTLSVDPDCRWCPAPDCGYAVIAHGCASCPKLQCGRPGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>H2XXY1</accession>
    <name>H2XXY1_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">wild type</strain>
        </source>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="EAAA01002038">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XXY1"/>
    <dbReference type="FunCoup" id="H2XXY1">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000034515"/>
    <dbReference type="Ensembl" id="ENSCINT00000033960.1">
        <property type="protein sequence ID" value="ENSCINP00000034515.1"/>
        <property type="gene ID" value="ENSCING00000018189.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164769"/>
    <dbReference type="HOGENOM" id="CLU_000288_63_0_1"/>
    <dbReference type="InParanoid" id="H2XXY1"/>
    <dbReference type="OMA" id="PNYQLIY"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007286">
        <property type="term" value="P:spermatid development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000943">
        <property type="entry name" value="testis-specific serine/threonine-protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF102">
        <property type="entry name" value="TESTIS-SPECIFIC SERINE_THREONINE-PROTEIN KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000654">
        <property type="entry name" value="Integrin-linked_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 18 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="11" id="KW-0221">Differentiation</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 18 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 19" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="15" id="KW-0744">Spermatogenesis</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="14" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Protein kinase" evidence="20">
        <location>
            <begin position="21"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
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                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
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                <person name="Francino P."/>
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                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1">wild type</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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    <reference evidence="1" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <property type="entry name" value="NADH_UbQ_FeS_4_mit-like"/>
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        <property type="entry name" value="WDR93_beta-prop"/>
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        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE"/>
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        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 93"/>
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    <dbReference type="Pfam" id="PF21030">
        <property type="entry name" value="WDR93"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    <sequence length="244" mass="27655" checksum="DDAFC5532083E3F9" modified="2012-04-18" version="1">VVSKKAGKDFSTTMPVYIRSGELNIPAPSISTVDDEDEDGVYLLDPELVVDELPQPFRMIDKVLTGIVEDVWDEIVDREKKREEEMRRVRPPVYNPTSKVEEFTNLSCMVHSNDARYMFVAFETGDLVAFDAVTHDRVAQWVSPDETICFEKLNCAVIGTQTYLLATIDDMGFSRLIVFTGDGFHVIQLLNEQPEGSPKSNAVKFQLSVNGDFCGLSLECEGVSWVDFYKLPRDNWLREIENAQ</sequence>
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<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>H2Y2A4</accession>
    <name>H2Y2A4_CIOIN</name>
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        <recommendedName>
            <fullName evidence="3">thioredoxin-disulfide reductase (NADPH)</fullName>
            <ecNumber evidence="3">1.8.1.9</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">wild type</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.8.1.9" evidence="3"/>
    <dbReference type="EMBL" id="EAAA01002606">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="interactions" value="455"/>
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    <dbReference type="STRING" id="7719.ENSCINP00000036039"/>
    <dbReference type="Ensembl" id="ENSCINT00000034883.1">
        <property type="protein sequence ID" value="ENSCINP00000036039.1"/>
        <property type="gene ID" value="ENSCING00000021016.1"/>
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        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="project" value="GO_Central"/>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="CDD" id="cd03419">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Glutaredoxin 2"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Thioredoxin reductase 1, cytoplasmic"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
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    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
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        <property type="entry name" value="Glutaredoxin"/>
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        <property type="entry name" value="GSHR/TRXR-like"/>
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        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
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    <dbReference type="InterPro" id="IPR012999">
        <property type="entry name" value="Pyr_OxRdtase_I_AS"/>
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        <property type="entry name" value="Thioredoxin-like_sf"/>
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        <property type="entry name" value="Thioredoxin/glutathione_Rdtase"/>
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        <property type="entry name" value="TGR"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GLUTATHIONE REDUCTASE"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Pyr_redox_2"/>
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    <keyword evidence="5 11" id="KW-0274">FAD</keyword>
    <keyword evidence="4 11" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="8 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10 11" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0712">Selenocysteine</keyword>
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        <location>
            <begin position="20"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="14">
        <location>
            <begin position="112"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="domain" description="Pyridine nucleotide-disulphide oxidoreductase dimerisation" evidence="13">
        <location>
            <begin position="469"/>
            <end position="580"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007532"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012610"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
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    </evidence>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
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                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
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                <person name="Huang W."/>
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                <person name="Spagnuolo A."/>
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                <person name="Zhang C."/>
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                <person name="Richardson P."/>
                <person name="Lucas S."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
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                <person name="Inaba K."/>
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            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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            <strain evidence="7">wild type</strain>
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                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
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                <person name="Doyle S."/>
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                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
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                <person name="Mochizuki Y."/>
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                <person name="Hyatt P.D."/>
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                <person name="Doggett N."/>
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                <person name="Richardson P."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
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                <person name="Ueno K."/>
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                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
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                <person name="Wiley G.B."/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
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                <person name="Hastings K.E."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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    <reference evidence="14" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
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    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>uridine in 28S rRNA + S-adenosyl-L-methionine = N(3)-methyluridine in 28S rRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:83635"/>
            <dbReference type="Rhea" id="RHEA-COMP:20178"/>
            <dbReference type="Rhea" id="RHEA-COMP:20181"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74502"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:83636"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="6">Interacts with INCA1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class IV-like SAM-binding methyltransferase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01000496">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002131245.1">
        <property type="nucleotide sequence ID" value="XM_002131209.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6QCQ5"/>
    <dbReference type="FunCoup" id="F6QCQ5">
        <property type="interactions" value="889"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000016935"/>
    <dbReference type="Ensembl" id="ENSCINT00000016935.3">
        <property type="protein sequence ID" value="ENSCINP00000016935.3"/>
        <property type="gene ID" value="ENSCING00000008293.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100187134"/>
    <dbReference type="KEGG" id="cin:100187134"/>
    <dbReference type="GeneTree" id="ENSGT00390000016537"/>
    <dbReference type="HOGENOM" id="CLU_017233_4_0_1"/>
    <dbReference type="InParanoid" id="F6QCQ5"/>
    <dbReference type="OMA" id="PIQPRLE"/>
    <dbReference type="OrthoDB" id="361029at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031616">
        <property type="term" value="C:spindle pole centrosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035198">
        <property type="term" value="F:miRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051661">
        <property type="term" value="P:maintenance of centrosome location"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035196">
        <property type="term" value="P:miRNA processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18086">
        <property type="entry name" value="HsC9orf114-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000170">
        <property type="entry name" value="SPOUT domain containing methyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003750">
        <property type="entry name" value="Put_MeTrfase-C9orf114-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12150">
        <property type="entry name" value="CLASS IV SAM-BINDING METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12150:SF13">
        <property type="entry name" value="METHYLTRANSFERASE C9ORF114-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02598">
        <property type="entry name" value="Methyltrn_RNA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="alpha/beta knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00062137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00075627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00078957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00079311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00093228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00093377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00093639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000016935.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="361" mass="40407" checksum="C0763DCCEA97BD9B" modified="2012-04-18" version="2">MNGPSKNKSEEKLKRKVEKKLHKEKRKIRKLEKQMILKKDFEKKKEKLEEPVLGDGRSYTVSVALPGSILDNAQSPELRTYLAGQIARACVVFQVDEIIVFDESASSGKDSSTQGDFTGVGKKGNCNIQLARILQYLECPQYLRKSFFPKHKDLQYAGVLNPLDCPHHMREQDNAEFREGVVVDRPIKPGKGSFVDCGTLKTVQIDKVLQPNIRVTVKIKEQKKKYILGEVVPPHYPRTKGGLYWGYNVRLANSLSSAIVDCPYSDTGKYDITIGTSDKGEPVADFKMPTFKHLLIVFGGLQGLEAGLEADEALGGDIELSLLFDKYLNTCPNQGSRTIRTEEAILVTMSTLWSQILSNQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>F6QZ03</accession>
    <name>F6QZ03_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">GOLD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">wild type</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Membrane</location>
            <topology evidence="7">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the EMP24/GP25L family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01000508">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="EAAA01000509">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6QZ03"/>
    <dbReference type="FunCoup" id="F6QZ03">
        <property type="interactions" value="328"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000016857"/>
    <dbReference type="Ensembl" id="ENSCINT00000016857.3">
        <property type="protein sequence ID" value="ENSCINP00000016857.3"/>
        <property type="gene ID" value="ENSCING00000008258.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159012"/>
    <dbReference type="HOGENOM" id="CLU_066963_2_2_1"/>
    <dbReference type="InParanoid" id="F6QZ03"/>
    <dbReference type="OMA" id="YYICISC"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005793">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015720">
        <property type="entry name" value="Emp24-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009038">
        <property type="entry name" value="GOLD_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22811">
        <property type="entry name" value="TRANSMEMBRANE EMP24 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01105">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01190">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50866">
        <property type="entry name" value="GOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012474826" description="GOLD domain-containing protein" evidence="8">
        <location>
            <begin position="16"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="GOLD" evidence="9">
        <location>
            <begin position="25"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000016857.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="214" mass="24896" checksum="83A8C458C2ED7670" modified="2012-04-18" version="2">RAVLIFLLFVHCSYAIYFHVGEKEKKCFIEELPEDTMVTGKYKAQLWDKRSNQYLDSTPGMGMHVEIKDPESKVLLSKSYGSEGAFTFTSHTPGEHQICIHSNSTKWSLFAGGRIRVHLDIQVGENTNDYSQIAAKDKLTELQLRVRQLLDQVQQIQKEQNYQRYREERFRQTSENTNARVLWWSLAQSAVLVIAGIWQMRHLKGFFEAKKFCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>F6R2K7</accession>
    <accession>A0A1W2WBE7</accession>
    <name>F6R2K7_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Succinate dehydrogenase [ubiquinone] cytochrome b small subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC100180725</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">wild type</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Membrane-anchoring subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). SDH also oxidizes malate to the non-canonical enol form of oxaloacetate, enol-oxaloacetate. Enol-oxaloacetate, which is a potent inhibitor of the succinate dehydrogenase activity, is further isomerized into keto-oxaloacetate.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate metabolism; tricarboxylic acid cycle.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of complex II composed of four subunits: the flavoprotein (FP) SDHA, iron-sulfur protein (IP) SDHB, and a cytochrome b560 composed of SDHC and SDHD.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Mitochondrion inner membrane</location>
            <topology evidence="1 15">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the CybS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01000471">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002128308.3">
        <property type="nucleotide sequence ID" value="XM_002128272.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6R2K7"/>
    <dbReference type="FunCoup" id="F6R2K7">
        <property type="interactions" value="296"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000018372"/>
    <dbReference type="Ensembl" id="ENSCINT00000018372.3">
        <property type="protein sequence ID" value="ENSCINP00000018372.3"/>
        <property type="gene ID" value="ENSCING00000009053.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100180725"/>
    <dbReference type="KEGG" id="cin:100180725"/>
    <dbReference type="GeneTree" id="ENSGT00390000010003"/>
    <dbReference type="HOGENOM" id="CLU_1610189_0_0_1"/>
    <dbReference type="InParanoid" id="F6R2K7"/>
    <dbReference type="OMA" id="AGCFICP"/>
    <dbReference type="OrthoDB" id="18577at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045273">
        <property type="term" value="C:respiratory chain complex II (succinate dehydrogenase)"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048039">
        <property type="term" value="F:ubiquinone binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006121">
        <property type="term" value="P:mitochondrial electron transport, succinate to ubiquinone"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1300.10">
        <property type="entry name" value="Fumarate reductase/succinate dehydrogenase, transmembrane subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007992">
        <property type="entry name" value="CybS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034804">
        <property type="entry name" value="SQR/QFR_C/D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13337">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13337:SF2">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE [UBIQUINONE] CYTOCHROME B SMALL SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05328">
        <property type="entry name" value="CybS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81343">
        <property type="entry name" value="Fumarate reductase respiratory complex transmembrane subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0249">Electron transport</keyword>
    <keyword evidence="15" id="KW-0349">Heme</keyword>
    <keyword evidence="14" id="KW-0408">Iron</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="14 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 15" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7 15" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 15" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5 15" id="KW-0813">Transport</keyword>
    <keyword evidence="15" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="70"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="127"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="14">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <note>ligand shared with SDHC</note>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>a ubiquinone</name>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <note>ligand shared with IP/SDHB</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR607992-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR607992-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU364031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000018372.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="166" mass="18524" checksum="879B72A80FDC419C" modified="2012-04-18" version="2">MNFAALRVVRCMKVQSIVAGHRVPHVLTSCIRQPIQTINTSSAHNEISSQESFELRHQVSSMKKNNTNWVMYRAGSVGLLAAVAGCFICPGNAIVDFATITLIVHHNYFGIKSVLADYMPLFFKDGFTNMVMIAWLIISIVTLGLLYAFNYNNIGFSKAVNNFFKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>F6R5K2</accession>
    <name>F6R5K2_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01000350">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6R5K2"/>
    <dbReference type="STRING" id="7719.ENSCINP00000020067"/>
    <dbReference type="Ensembl" id="ENSCINT00000020067.3">
        <property type="protein sequence ID" value="ENSCINP00000020067.3"/>
        <property type="gene ID" value="ENSCING00000009947.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000169803"/>
    <dbReference type="HOGENOM" id="CLU_881477_0_0_1"/>
    <dbReference type="InParanoid" id="F6R5K2"/>
    <dbReference type="OMA" id="CTIMELV"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043027">
        <property type="term" value="F:cysteine-type endopeptidase inhibitor activity involved in apoptotic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031398">
        <property type="term" value="P:positive regulation of protein ubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00022">
        <property type="entry name" value="BIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1170.10:FF:000003">
        <property type="entry name" value="E3 ubiquitin-protein ligase XIAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1170.10">
        <property type="entry name" value="Inhibitor Of Apoptosis Protein (2mihbC-IAP-1), Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001370">
        <property type="entry name" value="BIR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050784">
        <property type="entry name" value="IAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10044:SF139">
        <property type="entry name" value="DEATH-ASSOCIATED INHIBITOR OF APOPTOSIS 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10044">
        <property type="entry name" value="INHIBITOR OF APOPTOSIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00653">
        <property type="entry name" value="BIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00238">
        <property type="entry name" value="BIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57924">
        <property type="entry name" value="Inhibitor of apoptosis (IAP) repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01282">
        <property type="entry name" value="BIR_REPEAT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50143">
        <property type="entry name" value="BIR_REPEAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="145"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="274"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="149"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="274"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000020067.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="316" mass="35698" checksum="823FBE51AAF5C7A6" modified="2012-04-18" version="2">MAEIDRQDITGLASPSLTQHKEAKTQILPGRNCCASNSVTAFDCKHPYTKNMENFRERIRTFISWPRGVTKATPVEIAKAGFFYLKERDRAKCFYCNGGLQNWEPNDEPWFEHAKWFPNCDFLLREKGIEFVQNVAGRFPNLNRPLLNDQATPKPSTSNLQLPAPATSKQTEVPQNSASHIEITRPTLEEAMESELVLAVSGFGFERELVQLVVERLLQETGNVNCTIMELVTATQQLEQEQMEENAMEVVESFNVNQEKPEMACAQINLSTSPTDSFPMQTPGNFTEQQVPGSKKMMYVKQKEVSVPELSEQSLA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-03-21" modified="2025-06-18" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>F6RCC2</accession>
    <name>NNRD_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP-dependent (S)-NAD(P)H-hydrate dehydratase</fullName>
            <ecNumber evidence="1">4.2.1.93</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">ATP-dependent NAD(P)HX dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ORF">Cin.36927</name>
    </gene>
    <organism>
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ATP, which is converted to ADP. Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(6S)-NADHX + ATP = ADP + phosphate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19017"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:64074"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.2.1.93"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>(6S)-NADPHX + ATP = ADP + phosphate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32231"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.2.1.93"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NnrD/CARKD family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.93" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="F6RCC2"/>
    <dbReference type="SMR" id="F6RCC2"/>
    <dbReference type="FunCoup" id="F6RCC2">
        <property type="interactions" value="55"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000006335"/>
    <dbReference type="eggNOG" id="KOG3974">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="F6RCC2"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047453">
        <property type="term" value="F:ATP-dependent NAD(P)H-hydrate dehydratase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110051">
        <property type="term" value="P:metabolite repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046496">
        <property type="term" value="P:nicotinamide nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01171">
        <property type="entry name" value="YXKO-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1190.20:FF:000028">
        <property type="entry name" value="ATP-dependent (S)-NAD(P)H-hydrate dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01965">
        <property type="entry name" value="NADHX_dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000631">
        <property type="entry name" value="CARKD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00196">
        <property type="entry name" value="yjeF_cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12592:SF0">
        <property type="entry name" value="ATP-DEPENDENT (S)-NAD(P)H-HYDRATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12592">
        <property type="entry name" value="ATP-DEPENDENT (S)-NAD(P)H-HYDRATE DEHYDRATASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01256">
        <property type="entry name" value="Carb_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51383">
        <property type="entry name" value="YJEF_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000416162" description="ATP-dependent (S)-NAD(P)H-hydrate dehydratase">
        <location>
            <begin position="1"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="domain" description="YjeF C-terminal" evidence="1">
        <location>
            <begin position="61"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="214"/>
            <end position="220"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="256"/>
            <end position="260"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="275"/>
            <end position="284"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="285"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03157"/>
        </source>
    </evidence>
    <sequence length="359" mass="38630" checksum="6E30D27AF246B751" modified="2011-07-27" version="1">MSTILFYTRALIIGATKSQGVFGKTGTLVANISSNRGIISIVPRTNPPQKLLKMQEEENSLLEEARKVVPALSFGKHKGQAGRVGVIGGSEEYTGAPYFAAISAMKAGADLAHVFCSKSASTVIKSYSPELIVHPLLDVPNAVTLLDEWLPRIHSHVIGPGLGRVDATLNTVKEILIKLKKQEIPIVIDADGLFLITRDPSIIHGYTKAILTPNVVEFQRLSKSMNLNWESKDLNGSIMETVALSKALGGVTIVRKGEVDIVAAGDEVVTMDEIGSPRRCGGQGDLLSGVMALFSYWTHNSTCTPPPTLLAGYAACFLTKRCANQAFQKHGRSTATTDLISEINSVFVNNFENPTDPKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>F6RVU9</accession>
    <name>F6RVU9_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="F6RVU9"/>
    <dbReference type="STRING" id="7719.ENSCINP00000015793"/>
    <dbReference type="Ensembl" id="ENSCINT00000015793.3">
        <property type="protein sequence ID" value="ENSCINP00000015793.3"/>
        <property type="gene ID" value="ENSCING00000021119.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164251"/>
    <dbReference type="HOGENOM" id="CLU_803988_0_0_1"/>
    <dbReference type="InParanoid" id="F6RVU9"/>
    <dbReference type="OMA" id="NHYIVED"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000038">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000014">
        <property type="entry name" value="Latent-transforming growth factor beta-binding protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024731">
        <property type="entry name" value="NELL2-like_EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052080">
        <property type="entry name" value="vWF_C/EGF_Fibrillin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47333:SF4">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47333">
        <property type="entry name" value="VON WILLEBRAND FACTOR C AND EGF DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12947">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 8" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="292"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="10">
        <location>
            <begin position="9"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="10">
        <location>
            <begin position="54"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="10">
        <location>
            <begin position="99"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="10">
        <location>
            <begin position="140"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="10">
        <location>
            <begin position="181"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="10">
        <location>
            <begin position="222"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000015793.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="345" mass="37651" checksum="2CA27404155DB289" modified="2012-04-18" version="2">MGDGTNCTDINECIEPEGSAPLCPNHSSCINTHGSYHCDCDSGFKADMLGQCNDIDECASATNNSSVCNTNEMCVNEMGSYTCMCNKGYQRRTGTQCLDIDECALGKQCRNNSYCKNTIGSYACLCDTGYTKATNGQCQDINECLLPDHGCHSKATCYNLDGKYVCECNGGYMGNGTYCQNIDECLENTTDCHRDATCRDTEGFYNCICKQGYTGDGLYCTDLNECEDPNSCPAIGSECTNLPGSYSCACKQGYSGDGSQCSEKLPLRTSQISSRNPEAVNSEPSTSKNHSVMLGLFISAGMVAFLMVVFVIVRIYKRRKNKRHTLQTEGAANPMMNHYIVEDHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>F6RYR1</accession>
    <name>F6RYR1_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Carbohydrate kinase FGGY N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">wild type</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01002058">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6RYR1"/>
    <dbReference type="STRING" id="7719.ENSCINP00000015225"/>
    <dbReference type="Ensembl" id="ENSCINT00000015225.3">
        <property type="protein sequence ID" value="ENSCINP00000015225.3"/>
        <property type="gene ID" value="ENSCING00000007405.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01000000214434"/>
    <dbReference type="HOGENOM" id="CLU_009281_6_2_1"/>
    <dbReference type="InParanoid" id="F6RYR1"/>
    <dbReference type="OMA" id="QIWSAIC"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018484">
        <property type="entry name" value="FGGY_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43435:SF4">
        <property type="entry name" value="FGGY CARBOHYDRATE KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43435">
        <property type="entry name" value="RIBULOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00370">
        <property type="entry name" value="FGGY_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carbohydrate kinase FGGY N-terminal" evidence="3">
        <location>
            <begin position="4"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000015225.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="238" mass="26616" checksum="BFFA212C02003E93" modified="2012-04-18" version="2">MVRYYIGVDVGSHSVRAGLVDNDGTFIATAEEGITVVEPKTNQYIQSSDEIWRKCCSVIKSVVKNVDPSNVQGIGFDATCSLVVLDHDFKPVPLTNAKSNNDDFNIIMWMDHRAKEEADFINSTNHEVLKFVGGKISLEMQPPKLLWIKKNQPECWTKAHHFFDLSDFLTFKCTNSLTRSSCAVTCKWLYSSESGWNKSFWKEIGLEDLNDNNFSKIGNVILPPGTAIKGGLTQETAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>F6SAA4</accession>
    <accession>A0A1W2WH77</accession>
    <name>F6SAA4_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">RING-box protein 2</fullName>
            <ecNumber evidence="7">2.3.2.27</ecNumber>
            <ecNumber evidence="17">2.3.2.32</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">RING finger protein 7</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Sensitive to apoptosis gene protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="primary">LOC100181702</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>S-[NEDD8-protein]-yl-[E2 NEDD8-conjugating enzyme]-L-cysteine + [cullin]-L-lysine = [E2 NEDD8-conjugating enzyme]-L-cysteine + N(6)-[NEDD8-protein]-yl-[cullin]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.32"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Protein modification; protein neddylation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="18">Catalytic component of multiple cullin-5-RING E3 ubiquitin-protein ligase complexes (ECS complexes, also named CRL5 complexes) composed of CUL5, Elongin BC (ELOB and ELOC), RNF7/RBX2 and a variable SOCS box domain-containing protein as substrate-specific recognition component. Also interacts (with lower preference) with CUL1, CUL2, CUL3, CUL4A and CUL4B; additional evidence is however required to confirm this result in vivo. Interacts with UBE2F. Interacts with CSNK2B, the interaction is not affected by phosphorylation by CK2. May also interact with DCUN1D1, DCUN1D2, DCUN1D3, DCUN1D4 and DCUN1D5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the RING-box family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="7"/>
    <dbReference type="EC" id="2.3.2.32" evidence="17"/>
    <dbReference type="RefSeq" id="XP_002127625.1">
        <property type="nucleotide sequence ID" value="XM_002127589.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6SAA4"/>
    <dbReference type="FunCoup" id="F6SAA4">
        <property type="interactions" value="386"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000002660"/>
    <dbReference type="Ensembl" id="ENSCINT00000002660.3">
        <property type="protein sequence ID" value="ENSCINP00000002660.3"/>
        <property type="gene ID" value="ENSCING00000001372.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100181702"/>
    <dbReference type="KEGG" id="cin:100181702"/>
    <dbReference type="GeneTree" id="ENSGT00940000155481"/>
    <dbReference type="HOGENOM" id="CLU_115512_2_2_1"/>
    <dbReference type="InParanoid" id="F6SAA4"/>
    <dbReference type="OMA" id="RQNNRCP"/>
    <dbReference type="OrthoDB" id="8962942at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031466">
        <property type="term" value="C:Cul5-RING ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097602">
        <property type="term" value="F:cullin family protein binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061663">
        <property type="term" value="F:NEDD8 ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd16466">
        <property type="entry name" value="RING-H2_RBX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000156">
        <property type="entry name" value="RING-box protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051031">
        <property type="entry name" value="RING-box_E3_Ubiquitin_Ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024766">
        <property type="entry name" value="Znf_RING_H2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11210">
        <property type="entry name" value="RING BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12678">
        <property type="entry name" value="zf-rbx1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0007">Acetylation</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="12" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <keyword evidence="11 22" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="23">
        <location>
            <begin position="48"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00009273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00044896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00044971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00062884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00069656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00078874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00083543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000002660.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="100" mass="11421" checksum="EA823919189CF0A1" modified="2011-07-27" version="1">MADDASDNAHEKPTQLFTLKKWNAVAVWNWDVECDTCAICRVQVMDACLRCQADNKQDECVVVWGECNHSFHNCCMALWVEQNNRCPLCQKAWVIQGTGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>F6SKV4</accession>
    <accession>A0A1W3JIZ9</accession>
    <name>F6SKV4_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Signal recognition particle 14 kDa protein</fullName>
            <shortName evidence="9">SRP14</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC100184140</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">wild type</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8 9">Component of the signal recognition particle (SRP) complex, a ribonucleoprotein complex that mediates the cotranslational targeting of secretory and membrane proteins to the endoplasmic reticulum (ER). SRP9 together with SRP14 and the Alu portion of the SRP RNA, constitutes the elongation arrest domain of SRP. The complex of SRP9 and SRP14 is required for SRP RNA binding.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Heterodimer with SRP9; binds RNA as heterodimer. Component of a signal recognition particle (SRP) complex that consists of a 7SL RNA molecule of 300 nucleotides and six protein subunits: SRP72, SRP68, SRP54, SRP19, SRP14 and SRP9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the SRP14 family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01000153">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009862302.1">
        <property type="nucleotide sequence ID" value="XM_009864000.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6SKV4"/>
    <dbReference type="FunCoup" id="F6SKV4">
        <property type="interactions" value="668"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000010612"/>
    <dbReference type="Ensembl" id="ENSCINT00000010612.3">
        <property type="protein sequence ID" value="ENSCINP00000010612.3"/>
        <property type="gene ID" value="ENSCING00000005156.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100184140"/>
    <dbReference type="KEGG" id="cin:100184140"/>
    <dbReference type="GeneTree" id="ENSGT00390000008496"/>
    <dbReference type="HOGENOM" id="CLU_094309_2_1_1"/>
    <dbReference type="InParanoid" id="F6SKV4"/>
    <dbReference type="OMA" id="EQHIPND"/>
    <dbReference type="OrthoDB" id="19209at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005786">
        <property type="term" value="C:signal recognition particle, endoplasmic reticulum targeting"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008312">
        <property type="term" value="F:7S RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030942">
        <property type="term" value="F:endoplasmic reticulum signal peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045047">
        <property type="term" value="P:protein targeting to ER"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.720.10:FF:000003">
        <property type="entry name" value="Signal recognition particle 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.720.10">
        <property type="entry name" value="Signal recognition particle alu RNA binding heterodimer, srp9/1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003210">
        <property type="entry name" value="Signal_recog_particle_SRP14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009018">
        <property type="entry name" value="Signal_recog_particle_SRP9/14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12013">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE 14 KD PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02290">
        <property type="entry name" value="SRP14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54762">
        <property type="entry name" value="Signal recognition particle alu RNA binding heterodimer, SRP9/14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 9" id="KW-0733">Signal recognition particle</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="34"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00045462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU368100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000010612.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="119" mass="13301" checksum="75822E46DEB2CCE2" modified="2011-07-27" version="1">MVLLENDSFLTELTTLYQKCRTSGTVYVTMKKYDGRTKPKPKPNNLRSKQKPQVQAPIEGLCLIRATNGKKKLSTVVNAKDINKFQMAYTSVLRANLDGLKKHGKKKSKLASSSKVADQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>F6SLI9</accession>
    <name>F6SLI9_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized LOC100176601</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC100176601</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="F6SLI9"/>
    <dbReference type="Ensembl" id="ENSCINT00000027724.2">
        <property type="protein sequence ID" value="ENSCINP00000027478.2"/>
        <property type="gene ID" value="ENSCING00000024137.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000064660"/>
    <dbReference type="HOGENOM" id="CLU_093503_0_0_1"/>
    <dbReference type="InParanoid" id="F6SLI9"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038187">
        <property type="term" value="F:pattern recognition receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00037">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003341583" evidence="2">
        <location>
            <begin position="19"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="3">
        <location>
            <begin position="126"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="188"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000027478.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="209" mass="23621" checksum="F7EBF95CFA7555AB" modified="2012-04-18" version="2">MKCIWFVLLVEVISVVDSYRPLTSRGSIDLYLTSKLEIPYRMCMPKAPDYVHATARPSNPSLPHKFNVTILEIKKLSFIVEIERVDQAAGWDRMLITVDWSLYIGKGIGYQNLILWFPNAVDRRGMNRNTASKYCTDMGGRLVDIVDKAMYDVVYNYCRQTIVFGLQSATNIWLGSSYNTTTDTVTQSNGKPGYHGDWTPGGYPLRGSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F6SP14</accession>
    <accession>A0A1W2WJD0</accession>
    <name>F6SP14_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized LOC100175718</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC100175718</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">wild type</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="KEGG" id="cin:100175718"/>
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            <end position="139"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
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        <source>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">wild type</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
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            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000026909.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    </evidence>
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<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>F6SWC9</accession>
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        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">wild type</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002155">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6SWC9"/>
    <dbReference type="STRING" id="7719.ENSCINP00000014633"/>
    <dbReference type="ESTHER" id="cioin-f6swc9">
        <property type="family name" value="Pancreatic_lipase"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSCINT00000014633.3">
        <property type="protein sequence ID" value="ENSCINP00000014633.3"/>
        <property type="gene ID" value="ENSCING00000007118.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000169437"/>
    <dbReference type="HOGENOM" id="CLU_027171_0_0_1"/>
    <dbReference type="InParanoid" id="F6SWC9"/>
    <dbReference type="OMA" id="VIEWDYW"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016298">
        <property type="term" value="F:lipase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00707">
        <property type="entry name" value="Pancreat_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000033">
        <property type="entry name" value="Pancreatic triacylglycerol lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013818">
        <property type="entry name" value="Lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033906">
        <property type="entry name" value="Lipase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002331">
        <property type="entry name" value="Lipase_panc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000734">
        <property type="entry name" value="TAG_lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610">
        <property type="entry name" value="LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610:SF173">
        <property type="entry name" value="LIPASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00151">
        <property type="entry name" value="Lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00823">
        <property type="entry name" value="PANCLIPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00821">
        <property type="entry name" value="TAGLIPASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Lipase" evidence="6">
        <location>
            <begin position="25"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000014633.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="363" mass="40597" checksum="597F399C33EABBF1" modified="2012-04-18" version="2">FFKFLLSDLYFIPGKLTGTFRSSGEVCFDELGCFGDSRPFTSLQRPIPYLPWSPVELNTEFWLYTRLNRRTYNVLKYNDQTSINNSHFRAIRPTKFLVHGFVSSGETSWVQDMKNVLLDAEDVNVIVVNWEQGANPKLNYGQATANTRVVGAEIALLINRLEEQSGALEKNAHIIGHGLGAHVAGYAGERLKRLGRITGLDPAEPFYQGTDPVVRLDPTDALYVDAIHTDGKPYWQFGWGMMDPVGHADFYPNGGQDQPGCPGNEEESDFIIVFCKITSNWWEVTCNHGRSCELMIDSIVNAKTPMIGHPCADYDSYLTGKCDNCAEAGCATLGYYSDANAQKAVSVKYYLQTRSEVPFTFCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>F6T0K4</accession>
    <accession>A0A1W3JPY5</accession>
    <name>F6T0K4_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC104265829</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">wild type</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the DEAD box helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="EAAA01002479">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009859024.1">
        <property type="nucleotide sequence ID" value="XM_009860722.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6T0K4"/>
    <dbReference type="STRING" id="7719.ENSCINP00000017838"/>
    <dbReference type="Ensembl" id="ENSCINT00000017838.3">
        <property type="protein sequence ID" value="ENSCINP00000017838.3"/>
        <property type="gene ID" value="ENSCING00000008750.3"/>
    </dbReference>
    <dbReference type="GeneID" id="104265829"/>
    <dbReference type="KEGG" id="cin:104265829"/>
    <dbReference type="GeneTree" id="ENSGT00940000160049"/>
    <dbReference type="HOGENOM" id="CLU_003041_1_5_1"/>
    <dbReference type="InParanoid" id="F6T0K4"/>
    <dbReference type="OMA" id="DEMNIGM"/>
    <dbReference type="OrthoDB" id="196131at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000380">
        <property type="term" value="P:alternative mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000008">
        <property type="entry name" value="ATP-dependent RNA helicase RhlB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000079">
        <property type="entry name" value="probable ATP-dependent RNA helicase DDX17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <source>
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<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
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        <name evidence="9" type="primary">LOC100182035</name>
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        <name type="scientific">Ciona intestinalis</name>
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    <reference evidence="10" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">wild type</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CSN8 family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </evidence>
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        <source>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
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    <reference evidence="15" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="7">Component of the ribosome quality control complex (RQC), composed of the E3 ubiquitin ligase LTN1, TCF25 and NEMF associated with the 60S ribosomal subunit. The complex probably also contains VCP/p97 and its ubiquitin-binding cofactors. Interacts (via its N-terminus) with XPO1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
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            <location evidence="1">Nucleus</location>
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    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
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        <source>
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        <source>
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        <source>
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            <dbReference type="Pfam" id="PF05670"/>
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            <dbReference type="Pfam" id="PF11923"/>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>F6TI55</accession>
    <name>F6TI55_CIOIN</name>
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        <recommendedName>
            <fullName evidence="8">Sphingomyelin phosphodiesterase 4</fullName>
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    </protein>
    <organism evidence="6 7">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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            <taxon>Phlebobranchia</taxon>
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            <taxon>Ciona</taxon>
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    <reference evidence="7" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">wild type</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="ENSCINP00000002919.3"/>
        <property type="gene ID" value="ENSCING00000003912.3"/>
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    <dbReference type="InParanoid" id="F6TI55"/>
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    <dbReference type="Proteomes" id="UP000008144">
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    <dbReference type="GO" id="GO:0016020">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:sphingomyelin phosphodiesterase D activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046475">
        <property type="term" value="P:glycerophospholipid catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="P:sphingomyelin catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
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        <property type="entry name" value="Sphingomy_SMPD4"/>
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    <dbReference type="PANTHER" id="PTHR12988">
        <property type="entry name" value="SPHINGOMYELIN PHOSPHODIESTERASE 4"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR12988:SF6">
        <property type="entry name" value="SPHINGOMYELIN PHOSPHODIESTERASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="mit_SMPDase"/>
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    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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    <evidence type="ECO:0000256" key="1">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000002919.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="753" mass="85341" checksum="0D98B9CC841083EA" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>F6TJY9</accession>
    <name>F6TJY9_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">40S ribosomal protein S21</fullName>
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    </protein>
    <organism evidence="8 9">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
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        <lineage>
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            <taxon>Ciona</taxon>
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    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="4">Component of the 40S small ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Rough endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the eukaryotic ribosomal protein eS21 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="F6TJY9"/>
    <dbReference type="FunCoup" id="F6TJY9">
        <property type="interactions" value="473"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000007489"/>
    <dbReference type="Ensembl" id="ENSCINT00000007489.3">
        <property type="protein sequence ID" value="ENSCINP00000007489.3"/>
        <property type="gene ID" value="ENSCING00000003630.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000017515"/>
    <dbReference type="HOGENOM" id="CLU_167122_2_0_1"/>
    <dbReference type="InParanoid" id="F6TJY9"/>
    <dbReference type="OMA" id="GESDACM"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005791">
        <property type="term" value="C:rough endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000461">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1230.20:FF:000001">
        <property type="entry name" value="40S ribosomal protein S21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1230.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001931">
        <property type="entry name" value="Ribosomal_eS21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018279">
        <property type="entry name" value="Ribosomal_eS21_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038579">
        <property type="entry name" value="Ribosomal_eS21_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10442">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01249">
        <property type="entry name" value="Ribosomal_S21e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002148">
        <property type="entry name" value="Ribosomal_S21e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00996">
        <property type="entry name" value="RIBOSOMAL_S21E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 7" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR002148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000007489.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="79" mass="8590" checksum="6FF187FF962F0AE8" modified="2011-07-27" version="1">MQNDAGEVVDLYIPRKCSASGRIIGAKDHASVQLSFIDVDETGRALTTAKPYTICGDIRRMGESDDCLNRLAKKDGIMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>F6TYL9</accession>
    <name>F6TYL9_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tryptophan 2,3-dioxygenase</fullName>
            <shortName evidence="1">TDO</shortName>
            <ecNumber evidence="1">1.13.11.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Tryptamin 2,3-dioxygenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Tryptophan oxygenase</fullName>
            <shortName evidence="1">TO</shortName>
            <shortName evidence="1">TRPO</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Tryptophan pyrrolase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Tryptophanase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC100181631</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Heme-dependent dioxygenase that catalyzes the oxidative cleavage of the L-tryptophan (L-Trp) pyrrole ring and converts L-tryptophan to N-formyl-L-kynurenine. Catalyzes the oxidative cleavage of the indole moiety.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-tryptophan + O2 = N-formyl-L-kynurenine</text>
            <dbReference type="Rhea" id="RHEA:24536"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:58629"/>
            <dbReference type="EC" id="1.13.11.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
        <text evidence="1">Binds 1 heme group per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer. Dimer of dimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the tryptophan 2,3-dioxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.13.11.11" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="F6TYL9"/>
    <dbReference type="FunCoup" id="F6TYL9">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000025991"/>
    <dbReference type="Ensembl" id="ENSCINT00000026237.2">
        <property type="protein sequence ID" value="ENSCINP00000025991.2"/>
        <property type="gene ID" value="ENSCING00000005332.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000008593"/>
    <dbReference type="HOGENOM" id="CLU_045599_1_1_1"/>
    <dbReference type="InParanoid" id="F6TYL9"/>
    <dbReference type="OMA" id="WRWRNDH"/>
    <dbReference type="UniPathway" id="UPA00333">
        <property type="reaction ID" value="UER00453"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004833">
        <property type="term" value="F:L-tryptophan 2,3-dioxygenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019442">
        <property type="term" value="P:L-tryptophan catabolic process to acetyl-CoA"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019441">
        <property type="term" value="P:L-tryptophan catabolic process to kynurenine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.3810">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01972">
        <property type="entry name" value="T23O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037217">
        <property type="entry name" value="Trp/Indoleamine_2_3_dOase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004981">
        <property type="entry name" value="Trp_2_3_dOase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10138">
        <property type="entry name" value="TRYPTOPHAN 2,3-DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10138:SF0">
        <property type="entry name" value="TRYPTOPHAN 2,3-DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03301">
        <property type="entry name" value="Trp_dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140959">
        <property type="entry name" value="Indolic compounds 2,3-dioxygenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="1" id="KW-0349">Heme</keyword>
    <keyword evidence="1" id="KW-0408">Iron</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0823">Tryptophan catabolism</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000025991.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="412" mass="48133" checksum="880A9F006C1D4AFD" modified="2012-04-18" version="2">KMACPYLSGKKSKRRHSEPVLFDEGDFNLSSQEGKNKITAPSQLTYNSYLQLPRLLSSQYLQSSAGGKIPVHDEHLFIVTHQAIELWFKQIIYEVDSIRQIFADSYLEERNMLLVISRISRVSDILQILSDHFLVLETMTPQDFLDFRPFLMNGSGFQSLQFRLMEIKLGMPEDLRVQYNKQHYLEVFFGHEKETLKTALGQESLFSLVNAWLERTPGLEGNGFDFFNKFRCSVQAMHGEFEAFGEEIEDEEEKQEHQLEHSRGVETFNSIFDKNKHDELVARGDRRFSHKALQGALMIYLYRDEPRFHLPFQLLQVLMNIDSHLTKFRYSQTCMVQRMIGRKFGTGGSSGYLYLRSTCSDRYKIFLDLMNLSSFLVPRDRIPPLNQKTRRSLSIQDIYAPEVMRRVAEDQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>F6TZ79</accession>
    <name>F6TZ79_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
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    <dbReference type="AlphaFoldDB" id="F6TZ79"/>
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    <dbReference type="HOGENOM" id="CLU_1126685_0_0_1"/>
    <dbReference type="InParanoid" id="F6TZ79"/>
    <dbReference type="OMA" id="WIADCGR"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
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        <property type="entry name" value="ACYL-COENZYME A OXIDASE"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    </evidence>
    <sequence length="200" mass="22882" checksum="6227937ED2208BAF" modified="2012-04-18" version="2">MSPEQLRKEWSCTNDRSLTFDPSKMTKLLDHDNHEMRKKFREFMSDPVMTPRYDIPLAEEREVALQRLQRICDAKFISVTDFWTNPLRIFAAHEISAIIDPAMATKMTVQFNLFGGSVVKLGTKRHHDQLIKGIDTLDDVGCFGLTELGYGNNAVEMETTAVYDKESKEFIINTPTTLAQKYWITNGAVHAKHIIVFAQV</sequence>
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    <accession>F6U7M8</accession>
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        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Phlebobranchia</taxon>
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            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">wild type</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the fem-1 family.</text>
    </comment>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="Ensembl" id="ENSCINT00000027117.2">
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        <property type="gene ID" value="ENSCING00000006394.3"/>
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    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24173">
        <property type="entry name" value="ANKYRIN REPEAT CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24173:SF85">
        <property type="entry name" value="PROTEIN FEM-1 HOMOLOG CG6966"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
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        <property type="match status" value="4"/>
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        <property type="entry name" value="ANK_REPEAT"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0040">ANK repeat</keyword>
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    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
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        <location>
            <begin position="41"/>
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    <feature type="repeat" description="ANK" evidence="6">
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        <source>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00038500"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000026871.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="633" mass="70376" checksum="50D0B944E80985FD" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
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    <name>F6UB39_CIOIN</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
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        <name type="common">Transparent sea squirt</name>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
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                <person name="Miura S."/>
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                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
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                <person name="Oishi K."/>
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                <person name="Tokuoka M."/>
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                <person name="Wada S."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
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            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">wild type</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01001115">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="F6UB39"/>
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        <property type="protein sequence ID" value="ENSCINP00000026297.2"/>
        <property type="gene ID" value="ENSCING00000014579.2"/>
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        <property type="component" value="Chromosome 13"/>
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    <dbReference type="PANTHER" id="PTHR34400">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34400:SF4">
        <property type="entry name" value="MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
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    <evidence type="ECO:0000313" key="1">
        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    </evidence>
    <sequence length="206" mass="23109" checksum="E4BDE99050F618DC" modified="2011-07-27" version="1">MSTRDMNLLKYHEVVIVQKHNDQWKIVIGEFSKGVQVGIFSKVQLRLNLGQSKEYVSYISRFNKPLPNTQVNIEVCPANAAVICGQPSCKLPLSTPRDALKIEALSPLTNSKGLITHKLTAVKSPNGLRGCGLDGQLYMMKVQADVSIGGQMHSYLNGYFCGSKSTFYETVLWPLVRIYDETPVPVCPTWMDVRGIFTQYYNLYPV</sequence>
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<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
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    <name>F6UPP6_CIOIN</name>
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            <fullName evidence="2">Mediator of RNA polymerase II transcription subunit 22</fullName>
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        <alternativeName>
            <fullName evidence="7">Surfeit locus protein 5</fullName>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
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    <reference evidence="10" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
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                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
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                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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    <dbReference type="Ensembl" id="ENSCINT00000001602.3">
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        <property type="gene ID" value="ENSCING00000017420.2"/>
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    <dbReference type="InParanoid" id="F6UPP6"/>
    <dbReference type="OMA" id="ACENITR"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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    <dbReference type="GO" id="GO:0016592">
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        <property type="evidence" value="ECO:0000318"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 22"/>
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    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
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        <source>
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    </evidence>
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        <source>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
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                <person name="Oishi K."/>
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                <person name="Sasaki A."/>
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                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
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                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
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                <person name="Richardson P."/>
                <person name="Lucas S."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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            <strain evidence="2">wild type</strain>
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        <scope>IDENTIFICATION</scope>
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        <location>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000021728.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="169" mass="19756" checksum="98DD7ED534019577" modified="2012-04-18" version="2">MSKLGIFILLSACMFVSFRDVESINTAVVAGQQFLHYTLEKNVNNFNETERNKTCSMFIKDFKRFWYNSSGGKYLTEYLSFLEEWLCHAQFSLECLKRPYDFNQFTKTTYDRACNRSVFETQCFSSLVAVSSVRGRNSSQKWNNLLRALRTYQLSDAELQDPCIQVALY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>F6VC32</accession>
    <name>F6VC32_CIOIN</name>
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        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01000559">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6VC32"/>
    <dbReference type="Ensembl" id="ENSCINT00000027370.2">
        <property type="protein sequence ID" value="ENSCINP00000027124.2"/>
        <property type="gene ID" value="ENSCING00000015229.2"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2739255_0_0_1"/>
    <dbReference type="InParanoid" id="F6VC32"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003344520" description="Secreted protein" evidence="1">
        <location>
            <begin position="24"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000027124.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="71" mass="8015" checksum="C35A6554FFB14C8B" modified="2012-04-18" version="2">MNKSALLLLLVVGPLVLIGTTNATIVFPSRRYGSLWSSFRRRIIRIHPQPVWFYTPLKTASDGIDLSAFLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>F6VNS2</accession>
    <name>F6VNS2_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">receptor protein-tyrosine kinase</fullName>
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        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC100183420</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.10.1" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="F6VNS2"/>
    <dbReference type="STRING" id="7719.ENSCINP00000002277"/>
    <dbReference type="Ensembl" id="ENSCINT00000002277.3">
        <property type="protein sequence ID" value="ENSCINP00000002277.3"/>
        <property type="gene ID" value="ENSCING00000001197.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156107"/>
    <dbReference type="HOGENOM" id="CLU_1237468_0_0_1"/>
    <dbReference type="InParanoid" id="F6VNS2"/>
    <dbReference type="OMA" id="CMISEAV"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004714">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.20.20:FF:000052">
        <property type="entry name" value="Receptor protein-tyrosine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.220.10">
        <property type="entry name" value="Hormone Receptor, Insulin-like Growth Factor Receptor 1, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.20.20">
        <property type="entry name" value="Receptor L-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006211">
        <property type="entry name" value="Furin-like_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000494">
        <property type="entry name" value="Rcpt_L-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036941">
        <property type="entry name" value="Rcpt_L-dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00757">
        <property type="entry name" value="Furin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01030">
        <property type="entry name" value="Recep_L_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="Receptor L-domain" evidence="16">
        <location>
            <begin position="8"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="domain" description="Furin-like cysteine-rich" evidence="15">
        <location>
            <begin position="63"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00051243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000002277.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="224" mass="24484" checksum="7BC4195560258030" modified="2012-04-18" version="2">MNEQDSSQGLRELRLKSLTEIVSGGVKMKRNNHLCFTNTINWDDILRKETRNMYRVSLEDTPPPSCGSCDVTCGGGGCWGRGASMCQVLTSTICSEQCGNARCKGPAREDCCDIECASGCTGPSDKDCITCLHVNNTGACEYTCPPARIYDPLTQRNIPNPHFKFHYHDHCVDACPSNLLVEDNGCVKSCRRGLHNDGTGKCVQCSDELCSGDKECYGVNHQDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>F6VQ02</accession>
    <name>F6VQ02_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Serine-threonine kinase receptor-associated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100176230</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the WD repeat STRAP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="F6VQ02"/>
    <dbReference type="FunCoup" id="F6VQ02">
        <property type="interactions" value="537"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000000933"/>
    <dbReference type="Ensembl" id="ENSCINT00000000933.3">
        <property type="protein sequence ID" value="ENSCINP00000000933.3"/>
        <property type="gene ID" value="ENSCING00000000502.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155197"/>
    <dbReference type="HOGENOM" id="CLU_000288_57_6_1"/>
    <dbReference type="InParanoid" id="F6VQ02"/>
    <dbReference type="OMA" id="DGFYGLW"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032797">
        <property type="term" value="C:SMN complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030512">
        <property type="term" value="P:negative regulation of transforming growth factor beta receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000387">
        <property type="term" value="P:spliceosomal snRNP assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000133">
        <property type="entry name" value="Serine-threonine kinase receptor-associated protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19877">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19877:SF13">
        <property type="entry name" value="SERINE-THREONINE KINASE RECEPTOR-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 7" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="66"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="150"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="273"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00040390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000000933.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="328" mass="36231" checksum="B5D61A2963AD0947" modified="2012-04-18" version="2">TVSKSINQVINQMAKQTPLTCSGHTRPVVDLSFSNKTEDGYFLISACKDGKPMLRQGETGDWIGTFLGHKGAVWAATLNHNALRAATGAADFSANLWDAVKGDVITSYPHKHIVKAVAFSPDCTRLLTGGQEKIIRSFLIEKPDADPDIIGSHEGAIKKVIWGDDGRTMITAADDKSIRKWDPNSKNEVQRFDLESAIMDIELSKEGDIMLVTRSKSIAIYDTKLFTKLREFTMPTNAYSASLHPDKKFIVAGGEDFKLYKFDFQTLEELDSYKSHFGPVHIVRFSPDGELFASGSEDGTVRLWQTNVGTNYGLWRCGSGEDDTPIAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>F6VSC2</accession>
    <name>F6VSC2_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transporter</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="F6VSC2"/>
    <dbReference type="Ensembl" id="ENSCINT00000012631.3">
        <property type="protein sequence ID" value="ENSCINP00000012631.3"/>
        <property type="gene ID" value="ENSCING00000006122.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165957"/>
    <dbReference type="HOGENOM" id="CLU_006855_9_1_1"/>
    <dbReference type="InParanoid" id="F6VSC2"/>
    <dbReference type="OMA" id="CICKGVR"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005332">
        <property type="term" value="F:gamma-aminobutyric acid:sodium:chloride symporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd11496">
        <property type="entry name" value="SLC6sbd-TauT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000175">
        <property type="entry name" value="Na/ntran_symport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037272">
        <property type="entry name" value="SNS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616">
        <property type="entry name" value="SODIUM/CHLORIDE DEPENDENT TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616:SF325">
        <property type="entry name" value="TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00209">
        <property type="entry name" value="SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00176">
        <property type="entry name" value="NANEUSMPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161070">
        <property type="entry name" value="SNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00610">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00754">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50267">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0915">Sodium</keyword>
    <keyword evidence="8" id="KW-0769">Symport</keyword>
    <keyword evidence="3 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="17"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="47"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="89"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="189"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="220"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="272"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="300"/>
            <end position="325"/>
        </location>
    </feature>
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<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>F6WFF2</accession>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
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                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
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                <person name="Doggett N."/>
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                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
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    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
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                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
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                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
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                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
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                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
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                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
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                <person name="Levine M."/>
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            <dbReference type="PubMed" id="12481130"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="10" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
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            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">wild type</strain>
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    <reference evidence="10" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
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    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. OBG GTPase family.</text>
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        <property type="gene ID" value="ENSCING00000012641.2"/>
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    <dbReference type="GeneTree" id="ENSGT00940000155589"/>
    <dbReference type="HOGENOM" id="CLU_011747_2_3_1"/>
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    <dbReference type="OMA" id="VFMVDIF"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
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        <property type="term" value="C:nucleolus"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:GTP binding"/>
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        <property type="term" value="F:GTPase activity"/>
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        <property type="term" value="F:magnesium ion binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="CDD" id="cd01898">
        <property type="entry name" value="Obg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.210.12">
        <property type="entry name" value="GTP1/OBG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014100">
        <property type="entry name" value="GTP-bd_Obg/CgtA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006169">
        <property type="entry name" value="GTP1_OBG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036726">
        <property type="entry name" value="GTP1_OBG_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045086">
        <property type="entry name" value="OBG_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
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    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11702">
        <property type="entry name" value="DEVELOPMENTALLY REGULATED GTP-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11702:SF43">
        <property type="entry name" value="GTP-BINDING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01018">
        <property type="entry name" value="GTP1_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002401">
        <property type="entry name" value="GTP_bd_Obg/CgtA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82051">
        <property type="entry name" value="Obg GTP-binding protein N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51883">
        <property type="entry name" value="OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Obg" evidence="9">
        <location>
            <begin position="15"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="OBG-type G" evidence="8">
        <location>
            <begin position="155"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000023498.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="358" mass="39152" checksum="86465B9AFFA73A9C" modified="2012-04-18" version="2">MVFLTRALLLRTYSKDFIDKLRIYVRGGTGGNGHPTLGGIGGRGGDVYLVGATKSDMTLKSMKDKYPEKRFVADTGQSSRKQALSGLNGASIYVQVPHGISIVDAANNQVMEGEISGSLDKLLVARGGRGGTRSTGFIPRPGQRRNLYLDLKLIADVGFVGFPNAGKSTLLSRISKAKPKIASYPFTTITPQIGVLEYPDFRKIQLADLPGLVEGAYLNKGRGHSFLKHIERTKLLLFVVDISGFQLSTKHQFRNAFETVQLLTQELSSYDGTLTNKPSILAVNKMDLPAADDLFNNLLTSLQGNKRVPNFHLYVAASNFGSDCDEIMKFKDVIPISAHTGEGTEKLSSRIREIIDNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>F6WXR3</accession>
    <name>F6WXR3_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">wild type</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the carnitine/choline acetyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001251">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="EAAA01001252">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="EAAA01001253">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="EAAA01001254">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="EAAA01001255">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="EAAA01001256">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6WXR3"/>
    <dbReference type="FunCoup" id="F6WXR3">
        <property type="interactions" value="17"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000007072"/>
    <dbReference type="Ensembl" id="ENSCINT00000007072.3">
        <property type="protein sequence ID" value="ENSCINP00000007072.3"/>
        <property type="gene ID" value="ENSCING00000003450.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286917"/>
    <dbReference type="HOGENOM" id="CLU_013513_2_1_1"/>
    <dbReference type="InParanoid" id="F6WXR3"/>
    <dbReference type="OMA" id="AWIQMAL"/>
    <dbReference type="UniPathway" id="UPA00659"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004095">
        <property type="term" value="F:carnitine O-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009437">
        <property type="term" value="P:carnitine metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.10:FF:000042">
        <property type="entry name" value="Carnitine Palmitoyl Transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.70:FF:000021">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.70">
        <property type="entry name" value="Choline/Carnitine o-acyltransferase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000542">
        <property type="entry name" value="Carn_acyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039551">
        <property type="entry name" value="Cho/carn_acyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042231">
        <property type="entry name" value="Cho/carn_acyl_trans_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032476">
        <property type="entry name" value="CPT_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22589">
        <property type="entry name" value="CARNITINE O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22589:SF31">
        <property type="entry name" value="CARNITINE O-PALMITOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00755">
        <property type="entry name" value="Carn_acyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16484">
        <property type="entry name" value="CPT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="50"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="105"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="145"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="Carnitine O-palmitoyltransferase N-terminal" evidence="13">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="domain" description="Choline/carnitine acyltransferase" evidence="12">
        <location>
            <begin position="227"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10">
        <location>
            <position position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR600542-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF16484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000007072.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="764" mass="87156" checksum="8AB0BE1301CFE270" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>F6X4W9</accession>
    <name>F6X4W9_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">UTP--glucose-1-phosphate uridylyltransferase</fullName>
            <ecNumber evidence="3 9">2.7.7.9</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">UTP--glucose-1-phosphate uridylyltransferase catalyzing the conversion of glucose-1-phosphate into UDP-glucose, a crucial precursor for the production of glycogen.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>alpha-D-glucose 1-phosphate + UTP + H(+) = UDP-alpha-D-glucose + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19889"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.7.7.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:19890"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homooctamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the UDPGP type 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.9" evidence="3 9"/>
    <dbReference type="AlphaFoldDB" id="F6X4W9"/>
    <dbReference type="FunCoup" id="F6X4W9">
        <property type="interactions" value="184"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000015631"/>
    <dbReference type="Ensembl" id="ENSCINT00000015631.3">
        <property type="protein sequence ID" value="ENSCINP00000015631.3"/>
        <property type="gene ID" value="ENSCING00000015045.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153464"/>
    <dbReference type="HOGENOM" id="CLU_023632_3_0_1"/>
    <dbReference type="InParanoid" id="F6X4W9"/>
    <dbReference type="OMA" id="PSQHTED"/>
    <dbReference type="UniPathway" id="UPA00164"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003983">
        <property type="term" value="F:UTP:glucose-1-phosphate uridylyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005978">
        <property type="term" value="P:glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005977">
        <property type="term" value="P:glycogen metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006011">
        <property type="term" value="P:UDP-alpha-D-glucose metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00897">
        <property type="entry name" value="UGPase_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000002">
        <property type="entry name" value="UTP--glucose-1-phosphate uridylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002618">
        <property type="entry name" value="UDPGP_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016267">
        <property type="entry name" value="UDPGP_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43511">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01704">
        <property type="entry name" value="UDPGP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000806">
        <property type="entry name" value="UDPGP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="11">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="240"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="241"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="271"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="415"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR000806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000806-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000806-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000015631.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="466" mass="52625" checksum="D5B7BB48B5FC018B" modified="2012-04-18" version="2">MLGIRRQSSTNYVDWELLFSDPNLSPSRGSTMQRTQSIMEFQQLMKNDAKSALRYELDKLGNTAEKEYREIIKKDLANFETLFKRFLLKSGPSIVWNDIKSPPEGYIKSYSEISKPTLSGTEKHLLSKLVVVKLNGGLGTSMGCTGPKSLISVRSGLTFLDLTVQQIESLNKKYDAQVPLVLMNSFNTDDDTNMILRKYASCHVKIHTFNQNRYPRINKESLLPIAQSCNDPLEAWYPPGHGDVYECFVKSGLLDKFVKEGREFVFVSNIDNLGATIDLNILNMLVNPPEGSKPCDFVMEVTDKTRADVKGGTLIEYENKLRLLEIAQVPKEHVDEFKSVSKFKIFNTNNLWMSMQSISELTTAGKMEMEVIVNNKRLANGTNVIQLETASGAGIKNFHNAIGINVPRSRFLPVKKTSDLLLVMSNLYELKNGSMVMSSKRQFPTTPLVILVGSKYRKINQLLIYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>F6X5T4</accession>
    <name>F6X5T4_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="F6X5T4"/>
    <dbReference type="Ensembl" id="ENSCINT00000015607.3">
        <property type="protein sequence ID" value="ENSCINP00000015607.3"/>
        <property type="gene ID" value="ENSCING00000007613.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00730000113194"/>
    <dbReference type="HOGENOM" id="CLU_1717308_0_0_1"/>
    <dbReference type="InParanoid" id="F6X5T4"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000015607.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="153" mass="18650" checksum="B397B6A05647388D" modified="2011-07-27" version="1">CKRNDFRYERIEKLVKICKDRILELKSRLPWNKNAPERKVSYHCYINDIIKHKHVFSLLQDNYDSKQEDLTFLPPRYLRHTPMRYVAPKRSYDHVSTSEVISENEIPESEMKDYIRTPEEVAEVKKIEGFLVKKDEFEKRKSKPKKRRFVIKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>F6XAJ0</accession>
    <name>F6XAJ0_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
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            <taxon>Eukaryota</taxon>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
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                <person name="Hayashi H."/>
                <person name="Hino K."/>
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                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
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                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
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                <person name="Doggett N."/>
                <person name="Glavina T."/>
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                <person name="Richardson P."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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            <authorList>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
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                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
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            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
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    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
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                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Wang H.G."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
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                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
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                <person name="Wiley G.B."/>
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        <property type="entry name" value="HOMOCYSTEINE S-METHYLTRANSFERASE-LIKE ISOFORM 1"/>
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    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
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    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
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        <location>
            <begin position="5"/>
            <end position="256"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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        <source>
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        <source>
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    <accession>F6XFG7</accession>
    <name>F6XFG7_CIOIN</name>
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        <recommendedName>
            <fullName evidence="5">Coiled-coil domain-containing protein 83</fullName>
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    </protein>
    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
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            <taxon>Ciona</taxon>
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    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="Ensembl" id="ENSCINT00000016000.3">
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        <property type="gene ID" value="ENSCING00000007823.3"/>
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        <property type="entry name" value="CCDC83"/>
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    <dbReference type="PANTHER" id="PTHR21468:SF1">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 83"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HSD9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
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            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000016000.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="395" mass="46653" checksum="0D9202CE3DA6B766" modified="2012-04-18" version="1">MAKKGGKKKGKGGGGKGKKKGEKREDEMTLKEAILAFQIQVKEKASEDLQFEIKGLKDKNRRFRDRNARLYEDQQIHIKTLLRQAKEQEHELEEKEVYNSEHVEQALREKWAAARNEDKELDDLYKRIEQVEQEYAKMSSDVEYWTEYKDVGSEEHAKQIHLLNSELEDMLRNFNDMCGHLTRQLEAAKYEVTLYKEECMAEQQHLASEKAMEHLDPASVQEVHENEWLKKEAHIHREEEKNLLRLLDELESENLSIISELLYCKQEDLKISRNFYMTQFEEDEILNQDTMHDMDLKQMSVYQTKDDDNNSVKGKKILAIEPRPMSAMQRAVEQKVFSILPEQEEEEEEWENEIIARQDQDINIYEEKVILQDFLHLGPLEIKLLCVTGESKPIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F6XUK8</accession>
    <name>F6XUK8_CIOIN</name>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
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    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
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    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
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            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="Ensembl" id="ENSCINT00000023020.2">
        <property type="protein sequence ID" value="ENSCINP00000022774.2"/>
        <property type="gene ID" value="ENSCING00000012096.2"/>
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    <dbReference type="OMA" id="PRANDVT"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 5"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="87"/>
            <end position="110"/>
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    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="262"/>
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    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="6"/>
            <end position="64"/>
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    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="119"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="262"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="309" mass="35890" checksum="D6118A8E21524DC9" modified="2012-04-18" version="2">MFEQLKERHILRKREWKTEKEELLRQAAVAADSKKILLDLKKVLEGLRIELKEEERKRKEGSEIHQEEKRGWDEERTNLLDLIHELEKSSQRKPTNPQPSNPSEVDSLKRRLEVSDRDLNFMKKMNSEIEDENSKLKKEFESERIKLIKKHDDEEKKWSALKQQLLEKIQYLEEINMTPFPLHGSHVTSSCSDDRRHQTSLETTKPEVSMESKRIPAVLTTDKTASANLTTETCNYEQFHNTVDAALKQIEKISEDLDNINIEPTSPRANDVTSPEVETRNTSSLSSDLVAHPTMPRLADDIPQQAANF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>F6XVF3</accession>
    <name>F6XVF3_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="1">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">wild type</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPP phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="1"/>
    <dbReference type="EMBL" id="EAAA01000441">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6XVF3"/>
    <dbReference type="STRING" id="7719.ENSCINP00000010785"/>
    <dbReference type="Ensembl" id="ENSCINT00000010785.3">
        <property type="protein sequence ID" value="ENSCINP00000010785.3"/>
        <property type="gene ID" value="ENSCING00000005224.3"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004962_0_10_1"/>
    <dbReference type="InParanoid" id="F6XVF3"/>
    <dbReference type="OMA" id="LAQCKAR"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00144">
        <property type="entry name" value="MPP_PPP_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000058">
        <property type="entry name" value="Serine/threonine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050341">
        <property type="entry name" value="PP1_catalytic_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11668">
        <property type="entry name" value="SERINE/THREONINE PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11668:SF496">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="18"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="3">
        <location>
            <begin position="143"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000010785.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="345" mass="39687" checksum="C317BE97958C3050" modified="2012-04-18" version="2">ASKSNLVLMLHYKIFNHYFIRDFLMVMAFTFFYYNVTIVPTIKEVKTIFQKEDRLLKLQAPTYILGDIHGNHHDLVCFEKSLWRVGPLLTPCRFLFLGDYVDRGENGVEVRLLLYMISIYTVKQVVAYLLSQKILCPNKIFMVRGNHELRDIQVAFSFQTECVRKFGENLGIKVWEQINSCFDMLPIAAVVDNKIFTAHGGIPNSDTYASYNNVVEAINNIPTPLSNPEVESPLAWELMWNDPIRLDDYPLEELKDVSRSEGFVHNHRRKTASMFTSAALEKFLASNKFTHVIRAHEVQQIGFKVQQNGKLLTVFSSSRYCGGSNEAACILVDERKLRTIRLDTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>F6YD74</accession>
    <name>F6YD74_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">wild type</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01002953">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6YD74"/>
    <dbReference type="Ensembl" id="ENSCINT00000005430.3">
        <property type="protein sequence ID" value="ENSCINP00000005430.3"/>
        <property type="gene ID" value="ENSCING00000002665.3"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2372123_0_0_1"/>
    <dbReference type="InParanoid" id="F6YD74"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000005430.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="95" mass="10599" checksum="89AB2598A6C0EDA7" modified="2012-04-18" version="2">MLVASDQDQVEKLIVDSYTTREAMGQALSNVTPADLKEHHLNVMKKYLPQGLSFGAYDLKTEELIGLVLMGLPCEAKKQKLNDIVHCGFKPRVAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>F6YMT1</accession>
    <name>F6YMT1_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
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                <person name="Mount S."/>
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                <person name="Miura S."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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    <dbReference type="STRING" id="7719.ENSCINP00000028546"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="entry name" value="Surf2"/>
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        <property type="entry name" value="SURFEIT LOCUS PROTEIN 2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
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                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
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                <person name="Kobayashi K."/>
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                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
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                <person name="Mount S."/>
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                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
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                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
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                <person name="Richardson P."/>
                <person name="Lucas S."/>
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                <person name="Levine M."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">wild type</strain>
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    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <property type="entry name" value="L27 domain"/>
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        <location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000023400.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    <sequence length="247" mass="27795" checksum="DFBD07F92F65197F" modified="2012-04-18" version="2">MAMAEMPRSHNRYRLNGGPGTVLPPDETTHEMAVDCPAAYVAANPPRSGISTPRGNFDVPPPTPLDSTAVSSQKNKTTKDLEAAAAAAETLPQPDMNFQRIHRMRESVKRQKEQKLRDMEVRHQMKQEEGAKKLERLKHMTPEQEVKSRKQGFENYGFERDSYIHTNDEEKQPMGIESMFVALKNVGTKINDPSLSDDLSFLRHVVQDDDFQDSLELYNVITDSTKPKTSAPIIPVSKDMQTISSDV</sequence>
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    <reference evidence="10" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
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                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="9" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
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                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
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                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
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                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="9">wild type</strain>
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    <reference evidence="9" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
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        <property type="entry name" value="Fibrillin 2"/>
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    </evidence>
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        <source>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
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                <person name="Tassy O."/>
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                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000000599.3"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
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        <submittedName>
            <fullName evidence="3">Serine protease inhibitor Kazal-type 1-like</fullName>
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        <name evidence="3" type="primary">LOC100184703</name>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
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                <person name="Mount S."/>
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                <person name="Miura S."/>
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                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01002600">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002132087.4"/>
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    <dbReference type="AlphaFoldDB" id="F6YXM0"/>
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        <property type="protein sequence ID" value="ENSCINP00000024538.2"/>
        <property type="gene ID" value="ENSCING00000013340.2"/>
    </dbReference>
    <dbReference type="GeneID" id="100184703"/>
    <dbReference type="KEGG" id="cin:100184703"/>
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        <property type="component" value="Chromosome 8"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Kazal_dom"/>
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                <person name="Satou Y."/>
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                <person name="Chapman J."/>
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                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
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                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
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                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
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                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
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                <person name="Miura S."/>
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                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
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    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <source>
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    </evidence>
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                <person name="Meinertzhagen I.A."/>
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                <person name="Terry A."/>
                <person name="Yamada L."/>
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                <person name="Awazu S."/>
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                <person name="Branno M."/>
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                <person name="Makabe K.W."/>
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                <person name="Mount S."/>
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                <person name="Nomoto H."/>
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                <person name="Rigoutsos I."/>
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                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
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                <person name="Richardson P."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
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                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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            <dbReference type="PubMed" id="18854010"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">wild type</strain>
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    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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    </evidence>
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        <source>
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    <sequence length="227" mass="25529" checksum="B31BECDFBE1D4A4C" modified="2012-04-18" version="2">MTEVISMPILHSTYINIVNFLQSVTLKSAREKEEFFQMLLLKLKDSHIFAEVVAKRIIPLLLTPVILSDQYAVKHVIQHILCPVGETVTKRPKDGQGGVLPESTFARHVIPKIVELFQSRLIHVRMILVENFSKYVHLFEKSVLNNVVLPEILLGLSDSNNQLVQGSLRALADAVPVVGGDVIVGEEREKIFSHGVPKFDGRALNESQSLAFEVKLPPRKKLVRKQE</sequence>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
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                <person name="Hastings K.E."/>
                <person name="Ho I."/>
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                <person name="Huang W."/>
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                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
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                <person name="DeSantis R."/>
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                <person name="Francino P."/>
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                <person name="Hayashi H."/>
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                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
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                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
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                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
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                <person name="Richardson P."/>
                <person name="Lucas S."/>
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                <person name="Levine M."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01002545">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="F6ZA22"/>
    <dbReference type="Ensembl" id="ENSCINT00000026961.2">
        <property type="protein sequence ID" value="ENSCINP00000026715.2"/>
        <property type="gene ID" value="ENSCING00000014880.2"/>
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    <dbReference type="HOGENOM" id="CLU_1102463_0_0_1"/>
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        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
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        <location>
            <begin position="49"/>
            <end position="71"/>
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        <location>
            <begin position="107"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="49"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000026715.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    </evidence>
    <sequence length="252" mass="29507" checksum="EFF6012F580A76ED" modified="2012-04-18" version="2">MIIKSSRREDYDVEFELKHLREENRALKIKIKEKHNGSDYHPEYFAQNQQQQTNQQNKPEIQQTQQNSKDTEKFKCEIETLKQKVYETSQQSDEMRCRFEKTMATMKDNYDITIKEQEKKSENLEKKLMEVQSRLMTSQSECAISRTREVALQKQIGNLLSELQETRLGTPNAPMRRIENLNQQIQLLHFKQRDRENMIKKITGNTGSNEDVETLQAVIADKNLLLAKFRTELDLILGGLEKIKTKGVLGNA</sequence>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
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                <person name="Doyle S."/>
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                <person name="Keys D.N."/>
                <person name="Haga S."/>
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                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01001331">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F7A675"/>
    <dbReference type="STRING" id="7719.ENSCINP00000022167"/>
    <dbReference type="Ensembl" id="ENSCINT00000022413.2">
        <property type="protein sequence ID" value="ENSCINP00000022167.2"/>
        <property type="gene ID" value="ENSCING00000011641.2"/>
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        <property type="component" value="Chromosome 2"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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            <end position="195"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000022167.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    </evidence>
    <sequence length="227" mass="27139" checksum="D98F9F3B908A0E03" modified="2011-07-27" version="1">MERKLKERATNHADEVRLLYEEMEFQINEERRKTVAEEVVRERALSMNLQNELKQKERELERISQRQVALEKQVQELSSGKLEVRSENAQLVQLNQSLAEELQNTQKDLEETKVHVEAIERQKLQREEREQAEEEARKLDLEKKSLLRQLEMLREVNRKLRDDKDEAQQRNKLQTSTPKQRPPLFTQGSNVGKYFPEAEKHSEVYSNLHNLEEVRNIQRPKFEVIDE</sequence>
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<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
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    <accession>A0A1W2W0Y4</accession>
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        <submittedName>
            <fullName evidence="6">Ropporin-1-like protein</fullName>
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        <name evidence="6" type="primary">LOC100185860</name>
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    <organism evidence="6 7">
        <name type="scientific">Ciona intestinalis</name>
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    <reference evidence="7" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
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                <person name="Miura S."/>
                <person name="Nakayama A."/>
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                <person name="Nomoto H."/>
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                <person name="Yoshizaki F."/>
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                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
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                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
            <location evidence="1">Flagellum</location>
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        <text evidence="5">Belongs to the ropporin family.</text>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="12" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="12">wild type</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <text evidence="7">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs.</text>
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        <text evidence="7">Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits.</text>
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        <text evidence="2 7">Belongs to the eukaryotic RPC3/POLR3C RNA polymerase subunit family.</text>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
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                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
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                <person name="Inaba K."/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
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                <person name="Chapman J."/>
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                <person name="De Tomaso A."/>
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                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
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                <person name="Meinertzhagen I.A."/>
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                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
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                <person name="Makabe K.W."/>
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                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
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                <person name="Miura S."/>
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                <person name="Nishizaka S."/>
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                <person name="Yoshizaki F."/>
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                <person name="Zhang C."/>
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                <person name="Doggett N."/>
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                <person name="Richardson P."/>
                <person name="Lucas S."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
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                <person name="Lemaire P."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">wild type</strain>
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        <scope>IDENTIFICATION</scope>
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                <consortium name="Ensembl"/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
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                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
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                <person name="Meinertzhagen I.A."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
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                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">wild type</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs. The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing. Within the 17S U2 SnRNP complex, SF3A1 is part of the SF3A subcomplex that contributes to the assembly of the 17S U2 snRNP, and the subsequent assembly of the pre-spliceosome 'E' complex and the pre-catalytic spliceosome 'A' complex. Involved in pre-mRNA splicing as a component of pre-catalytic spliceosome 'B' complexes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Component of the 17S U2 SnRNP complex, a ribonucleoprotein complex that contains small nuclear RNA (snRNA) U2 and a number of specific proteins. Part of the SF3A subcomplex of the 17S U2 SnRNP complex which is composed of three subunits; SF3A3/SAP61, SF3A2/SAP62 and SF3A1/SAP114. SF3A associates with the splicing factor SF3B and a 12S RNA unit to form the mature 17S U2 small nuclear ribonucleoprotein complex (17S U2 snRNP). SF3A1 functions as a scaffold that interacts directly with both SF3A2 and SF3A3. Identified in the spliceosome 'E' complex, a precursor of the spliceosome 'A' complex. Identified in the spliceosome 'A' and 'B' complexes. Identified in the spliceosome 'C' complex. Interacts with P2RX6; resulting in a reduction of the splicing activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus speckle</location>
        </subcellularLocation>
    </comment>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="ENSCINP00000017384.3"/>
        <property type="gene ID" value="ENSCING00000008526.3"/>
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    <dbReference type="GeneTree" id="ENSGT00730000111077"/>
    <dbReference type="HOGENOM" id="CLU_013259_1_0_1"/>
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        <property type="component" value="Chromosome 11"/>
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        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
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    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
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    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
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        <property type="term" value="C:U2-type prespliceosome"/>
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        <property type="term" value="F:RNA binding"/>
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        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
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        <property type="entry name" value="Ubl_SF3a120"/>
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        <property type="entry name" value="Splicing factor 3A subunit 1"/>
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        <property type="entry name" value="Splicing factor 3A subunit 1"/>
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        <property type="entry name" value="Splicing factor 3a, subunit 1"/>
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    </dbReference>
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        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
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        <property type="entry name" value="SF3A1"/>
    </dbReference>
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        <property type="entry name" value="SF3A1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035563">
        <property type="entry name" value="SF3As1_ubi"/>
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    <dbReference type="InterPro" id="IPR000061">
        <property type="entry name" value="Surp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035967">
        <property type="entry name" value="SWAP/Surp_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15316">
        <property type="entry name" value="SPLICEOSOME ASSOCIATED PROTEIN 114/SWAP SPLICING FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15316:SF1">
        <property type="entry name" value="SPLICING FACTOR 3A SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12230">
        <property type="entry name" value="PRP21_like_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01805">
        <property type="entry name" value="Surp"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00648">
        <property type="entry name" value="SWAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109905">
        <property type="entry name" value="Surp module (SWAP domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50128">
        <property type="entry name" value="SURP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="2" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="9" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="SURP motif" evidence="16">
        <location>
            <begin position="42"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="SURP motif" evidence="16">
        <location>
            <begin position="156"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="15">
        <location>
            <begin position="696"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="300"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="556"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="300"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="354"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="564"/>
            <end position="577"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00060058"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00061882"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00074916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50053"/>
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    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50128"/>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000017384.3"/>
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    <evidence type="ECO:0000313" key="18">
        <source>
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</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>F7AXJ1</accession>
    <accession>A0A1W2WJ57</accession>
    <name>F7AXJ1_CIOIN</name>
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        <submittedName>
            <fullName evidence="7">ATP-binding cassette sub-family F member 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100185135</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">wild type</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily. ABCF family. EF3 subfamily.</text>
    </comment>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_002129929.5"/>
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        <property type="protein sequence ID" value="ENSCINP00000007529.3"/>
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        <property type="component" value="Chromosome 3"/>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03221">
        <property type="entry name" value="ABCF_EF-3"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="ATP-binding cassette sub-family F member 3"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="3.40.50.300:FF:000011">
        <property type="entry name" value="Putative ABC transporter ATP-binding component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
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    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
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        <property type="entry name" value="ABC_tran_Xtn"/>
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    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
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        <property type="term" value="P:establishment of mitotic spindle orientation"/>
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        <property type="term" value="P:vesicle transport along microtubule"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="NUDE"/>
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    <dbReference type="InterPro" id="IPR006964">
        <property type="entry name" value="NUDE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10921:SF1">
        <property type="entry name" value="NUCLEAR DISTRIBUTION PROTEIN NUDE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10921">
        <property type="entry name" value="NUCLEAR DISTRIBUTION PROTEIN NUDE HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04880">
        <property type="entry name" value="NUDE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NUDE" evidence="10">
        <location>
            <begin position="137"/>
            <end position="304"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="9">
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            <begin position="39"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
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            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
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            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
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            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00007429"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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            <dbReference type="Pfam" id="PF04880"/>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    </evidence>
    <sequence length="329" mass="37653" checksum="2E4FD71333EC6995" modified="2012-04-18" version="2">MAELQSEPPAFANAEEKAAYWEGEAKRFEEMTLEVQLELQEFRECSEEIEKELEAQLEQHEKQTKDLRQQNTTLTFEYESLKERYEKQQTERFRQVSDLQTEISKLTVTNGELTKDVRELEQRNDDLERTNRVIIVSLDDFEQRLNQALERNAFLESELDEKDSLSVTVQRLRDEARDLHHELSVRQRKVSESGKHDERSPLPSPQDKVPTPTTGVPTTPLATITTSHAPPSPTNPDPHKIHQKHVLHNGVDKTPLPPSARISALNIVGDLLRKFGALESKLASCRNFVHEQPPWQSKRVNQIAPKSSETPHEGSTTDSSKNSSVKIRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>F7B7H5</accession>
    <name>F7B7H5_CIOIN</name>
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        <recommendedName>
            <fullName evidence="4 13">Catalase</fullName>
            <ecNumber evidence="13">1.11.1.6</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the degradation of hydrogen peroxide (H(2)O(2)) generated by peroxisomal oxidases to water and oxygen, thereby protecting cells from the toxic effects of hydrogen peroxide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>2 H2O2 = O2 + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:20309"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="EC" id="1.11.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Peroxisome matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the catalase family.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.6" evidence="13"/>
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        <property type="interactions" value="49"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000010672"/>
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        <property type="protein sequence ID" value="ENSCINP00000010672.3"/>
        <property type="gene ID" value="ENSCING00000005187.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000018100"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="evidence" value="ECO:0000318"/>
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        <property type="project" value="GO_Central"/>
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        <property type="term" value="C:peroxisomal matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:peroxisome"/>
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        <property type="project" value="GO_Central"/>
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        <property type="term" value="F:catalase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0000318"/>
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        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="P:response to copper ion"/>
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        <property type="term" value="P:response to hydrogen peroxide"/>
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        <property type="project" value="GO_Central"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Catalase_AS"/>
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        <property type="entry name" value="Catalase_clade1/3"/>
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        <property type="entry name" value="Catalase_core"/>
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    <dbReference type="InterPro" id="IPR002226">
        <property type="entry name" value="Catalase_haem_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010582">
        <property type="entry name" value="Catalase_immune_responsive"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020835">
        <property type="entry name" value="Catalase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11465">
        <property type="entry name" value="CATALASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CATALASE"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00199">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038928">
        <property type="entry name" value="Catalase_clade1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00067">
        <property type="entry name" value="CATALASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01060">
        <property type="entry name" value="Catalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56634">
        <property type="entry name" value="Heme-dependent catalase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00437">
        <property type="entry name" value="CATALASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00438">
        <property type="entry name" value="CATALASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51402">
        <property type="entry name" value="CATALASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12 13" id="KW-0349">Heme</keyword>
    <keyword evidence="10 13" id="KW-0376">Hydrogen peroxide</keyword>
    <keyword evidence="9 12 13" id="KW-0408">Iron</keyword>
    <keyword evidence="7 12 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5 13" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Catalase core" evidence="15">
        <location>
            <begin position="24"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="71"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="144"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="12">
        <location>
            <position position="354"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004253"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
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        <source>
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        <source>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR038928-2"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU000498"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM01060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000010672.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="521" mass="58496" checksum="143DCFD48C080A88" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>H2XK79</accession>
    <name>H2XK79_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">ADP/ATP translocase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">ADP,ATP carrier protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">ADP:ATP antiporter that mediates import of ADP into the mitochondrial matrix for ATP synthesis, and export of ATP out to fuel the cell. Cycles between the cytoplasmic-open state (c-state) and the matrix-open state (m-state): operates by the alternating access mechanism with a single substrate-binding site intermittently exposed to either the cytosolic (c-state) or matrix (m-state) side of the inner mitochondrial membrane.</text>
    </comment>
    <comment type="function">
        <text evidence="17">Catalyzes the exchange of ADP and ATP across the membrane.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ADP(in) + ATP(out) = ADP(out) + ATP(in)</text>
            <dbReference type="Rhea" id="RHEA:34999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:35000"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>H(+)(in) = H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34979"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3 17">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="17">Membrane</location>
            <topology evidence="17">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="H2XK79"/>
    <dbReference type="FunCoup" id="H2XK79">
        <property type="interactions" value="130"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000030061"/>
    <dbReference type="Ensembl" id="ENSCINT00000036585.1">
        <property type="protein sequence ID" value="ENSCINP00000030061.1"/>
        <property type="gene ID" value="ENSCING00000023431.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160648"/>
    <dbReference type="HOGENOM" id="CLU_015166_12_0_1"/>
    <dbReference type="InParanoid" id="H2XK79"/>
    <dbReference type="OMA" id="NFACKEK"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005471">
        <property type="term" value="F:ATP:ADP antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140021">
        <property type="term" value="P:mitochondrial ADP transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990544">
        <property type="term" value="P:mitochondrial ATP transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901029">
        <property type="term" value="P:negative regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000002">
        <property type="entry name" value="Putative ADP/ATP translocase 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002113">
        <property type="entry name" value="ADT_euk_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45635">
        <property type="entry name" value="ADP,ATP CARRIER PROTEIN 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45635:SF14">
        <property type="entry name" value="ADP_ATP TRANSLOCASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00927">
        <property type="entry name" value="ADPTRNSLCASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0050">Antiport</keyword>
    <keyword evidence="11 15 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 15 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 16" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="174"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="212"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="270"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="15">
        <location>
            <begin position="8"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="15">
        <location>
            <begin position="113"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="15">
        <location>
            <begin position="213"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00024169"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00045250"/>
        </source>
    </evidence>
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            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU368008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000030061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="301" mass="32921" checksum="6AB024F16BCC0049" modified="2012-04-18" version="1">SKMPFNAMNFAQDLAVGGTAAAISKTLVAPIERVKLLLQVQAVSKQIAVEDQYKGIVDCFVRIPKEQGFASFWRGNLANVIRYFPTQALNFAFKDTYKQLFLAGVDKKTQFGRYFLGNLASGGAAGATGLCFVYPLDFARTRLAADVGSGKQREFSGLGNCIVTIAKRDGLRGLYQGFGVSIQGIIVYRAAYFGTYDTVKGMLPDPKNTPIVVSWAIAQVVTTGAGILSYPFDTVRRRLMMQSGRAKEDMPYKGTLDCWRKIKQQEGGKAFFKGAFSNILRGTGGALVLVLYDELKSLFFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>H2XKH2</accession>
    <name>H2XKH2_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="H2XKH2"/>
    <dbReference type="Ensembl" id="ENSCINT00000031575.1">
        <property type="protein sequence ID" value="ENSCINP00000030154.1"/>
        <property type="gene ID" value="ENSCING00000024814.1"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3086496_0_0_1"/>
    <dbReference type="InParanoid" id="H2XKH2"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000030154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="52" mass="5947" checksum="5CC99682E229F042" modified="2012-04-18" version="1">MISLSLYDFQDIYVVSHNNQNTSTTLRYFAFHDVNNIGSACHCDVIYATKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>H2XKN7</accession>
    <name>H2XKN7_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Eukaryotic translation initiation factor 4E binding protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
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                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <text evidence="1">Belongs to the eIF4E-binding protein family.</text>
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    <dbReference type="AlphaFoldDB" id="H2XKN7"/>
    <dbReference type="FunCoup" id="H2XKN7">
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        <property type="evidence" value="ECO:0000318"/>
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        <property type="term" value="F:translation repressor activity"/>
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        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E-BINDING PROTEIN"/>
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        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E-BINDING PROTEIN"/>
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        <location>
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    </evidence>
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    </evidence>
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        <source>
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        <source>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    </evidence>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
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                <person name="Haga S."/>
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                <person name="Imai K.S."/>
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                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
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    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="gene ID" value="ENSCING00000020621.1"/>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000030265.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
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    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
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                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
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                <person name="Francino P."/>
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                <person name="Kobayashi M."/>
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                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="7" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="7">wild type</strain>
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    <reference evidence="7" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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            <topology evidence="1">Multi-pass membrane protein</topology>
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        <location>
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        <source>
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        <source>
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        <source>
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    </evidence>
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        <source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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            <fullName evidence="5">Glucose-methanol-choline oxidoreductase N-terminal domain-containing protein</fullName>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">wild type</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GMC oxidoreductase family.</text>
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        <property type="gene ID" value="ENSCING00000020720.1"/>
    </dbReference>
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    <dbReference type="HOGENOM" id="CLU_131258_0_0_1"/>
    <dbReference type="InParanoid" id="H2XME9"/>
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        <property type="component" value="Chromosome 10"/>
    </dbReference>
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    </dbReference>
    <dbReference type="GO" id="GO:0016614">
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FAD/NAD-bd_sf"/>
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        <property type="entry name" value="GMC_OxRdtase"/>
    </dbReference>
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        <property type="entry name" value="GMC_OxRdtase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552:SF147">
        <property type="entry name" value="CHOLINE DEHYDROGENASE, MITOCHONDRIAL"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552">
        <property type="entry name" value="GLUCOSE-METHANOL-CHOLINE GMC OXIDOREDUCTASE"/>
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        </location>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
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</entry>
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    <accession>H2XML1</accession>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="H2XML1"/>
    <dbReference type="Ensembl" id="ENSCINT00000035668.1">
        <property type="protein sequence ID" value="ENSCINP00000030894.1"/>
        <property type="gene ID" value="ENSCING00000018137.1"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3379429_0_0_1"/>
    <dbReference type="InParanoid" id="H2XML1"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000030894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="34" mass="4115" checksum="D15A9B429F25D7C1" modified="2012-04-18" version="1">MTLFKTIIWKNVEGILPTYIVMERFCFRTVFTCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>H2XN09</accession>
    <name>H2XN09_CIOIN</name>
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        <recommendedName>
            <fullName evidence="10">Hexosyltransferase</fullName>
            <ecNumber evidence="10">2.4.1.-</ecNumber>
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    <gene>
        <name evidence="11" type="primary">LOC100184787</name>
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    <organism evidence="11 12">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Golgi apparatus membrane</location>
            <topology evidence="1 10">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the glycosyltransferase 31 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="10"/>
    <dbReference type="AlphaFoldDB" id="H2XN09"/>
    <dbReference type="STRING" id="7719.ENSCINP00000031042"/>
    <dbReference type="Ensembl" id="ENSCINT00000030527.1">
        <property type="protein sequence ID" value="ENSCINP00000031042.1"/>
        <property type="gene ID" value="ENSCING00000023331.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163442"/>
    <dbReference type="HOGENOM" id="CLU_036849_4_0_1"/>
    <dbReference type="InParanoid" id="H2XN09"/>
    <dbReference type="OMA" id="HYSGYTQ"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016758">
        <property type="term" value="F:hexosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002659">
        <property type="entry name" value="Glyco_trans_31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214">
        <property type="entry name" value="BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214:SF283">
        <property type="entry name" value="N-ACETYLLACTOSAMINIDE BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE 4-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01762">
        <property type="entry name" value="Galactosyl_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8 10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000031042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="332" mass="38323" checksum="89A1809B653E47CC" modified="2012-04-18" version="1">MQRKCSILTTLILVVGVVTVCRIVFEVNQSIKLGQTHTDQIYLKEPWALFKNRCSSPVCTGNASVGNVTWSMVIFVKSSANSFIRREILRRSWASLFRVEEGNITVVFVVGLSGARFTERRLTKESKEHGDILQFNGPDDYRNIALKTLAGMEWASLHLPSTFLYSSMDDDFMVDMLKMTKTINKGFELMRKDSWFEFPIICMFRMGVDEKPVRDENGIFKKWFISESLFKWPVFPRYCHGGMYTTSMRIIDQLLRESWSVDSLYLDDVWITGILRQKIGMPDQMVIKPEETVAVHYSGYTQSPNHNVNAFVDAWQQSIDEMVDSSVCQCFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>H2XPF4</accession>
    <name>H2XPF4_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Endoplasmic reticulum-Golgi intermediate compartment protein 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">wild type</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum-Golgi intermediate compartment membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ERGIC family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002013">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XPF4"/>
    <dbReference type="FunCoup" id="H2XPF4">
        <property type="interactions" value="130"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000031537"/>
    <dbReference type="Ensembl" id="ENSCINT00000031744.1">
        <property type="protein sequence ID" value="ENSCINP00000031537.1"/>
        <property type="gene ID" value="ENSCING00000022383.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063113"/>
    <dbReference type="HOGENOM" id="CLU_034705_4_1_1"/>
    <dbReference type="InParanoid" id="H2XPF4"/>
    <dbReference type="OMA" id="MTNHYLR"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033116">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045888">
        <property type="entry name" value="Erv"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012936">
        <property type="entry name" value="Erv_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039542">
        <property type="entry name" value="Erv_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10984">
        <property type="entry name" value="ENDOPLASMIC RETICULUM-GOLGI INTERMEDIATE COMPARTMENT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10984:SF30">
        <property type="entry name" value="ENDOPLASMIC RETICULUM-GOLGI INTERMEDIATE COMPARTMENT PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07970">
        <property type="entry name" value="COPIIcoated_ERV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13850">
        <property type="entry name" value="ERGIC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="319"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="Endoplasmic reticulum vesicle transporter N-terminal" evidence="9">
        <location>
            <begin position="15"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="Endoplasmic reticulum vesicle transporter C-terminal" evidence="8">
        <location>
            <begin position="172"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000031537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="340" mass="38397" checksum="A41CFB382BEEDF5E" modified="2012-04-18" version="1">MLRYRGPSVKSLSNVKSLDAFPKVPELCIETSTRGGTITLITTAVITFLVLSEIIYYFNVTFRYDYQVDVDFDSKVWLNFDITVATPCTLIGADVLDVTGQATVFENEVYEELTFFRQSNTAAAQRKALLRMKEELLTPENGKKMSEITLQSNFNPNLMFKNRKLDNVGIKMDACRFYGNLPLNKVAGNFHIVAGKPIQMFGGHAHLSMMFSPIPYNFSHRIDHFSFGNMKTGFINALDGDERVTSSESYIFQYYLDVVSTKINSRRITTDTFQFSVSEQSRALDHASGSHGQPGVFFKYNFSPLSVMITEQKMPFYRLLVRLCSIVGGIFATSHVLNAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>H2XPU5</accession>
    <name>H2XPU5_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01002225">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XPU5"/>
    <dbReference type="Ensembl" id="ENSCINT00000032918.1">
        <property type="protein sequence ID" value="ENSCINP00000031679.1"/>
        <property type="gene ID" value="ENSCING00000023449.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063883"/>
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    <dbReference type="InParanoid" id="H2XPU5"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004620">
        <property type="term" value="F:phospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038885">
        <property type="entry name" value="PLB1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21325:SF31">
        <property type="entry name" value="GH22081P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21325">
        <property type="entry name" value="PHOSPHOLIPASE B, PLB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="92"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="48"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000031679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="134" mass="14331" checksum="DE0E86CF8805D692" modified="2012-04-18" version="1">MAAVNLWNNMMEPVGSKTTVWGITDLKCPTQENPYIFTSKNSKPAIVPTDSPTTTTTTTKPTTTKKPAATSPAQTDPTVVCNETCSQVVPDWGIALLVVTGVALLLQMLVVICITSRKHSKPKAAEVYKNGIAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>H2XQL0</accession>
    <accession>A0A1W2WDK8</accession>
    <name>H2XQL0_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">wild type</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RNase K family.</text>
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    <dbReference type="EMBL" id="EAAA01002354">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="H2XQL0"/>
    <dbReference type="FunCoup" id="H2XQL0">
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    <dbReference type="STRING" id="7719.ENSCINP00000031944"/>
    <dbReference type="Ensembl" id="ENSCINT00000037078.1">
        <property type="protein sequence ID" value="ENSCINP00000031944.1"/>
        <property type="gene ID" value="ENSCING00000020541.1"/>
    </dbReference>
    <dbReference type="GeneID" id="100177179"/>
    <dbReference type="KEGG" id="cin:100177179"/>
    <dbReference type="GeneTree" id="ENSGT00940000171373"/>
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    <dbReference type="OMA" id="SNNCFIA"/>
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        <property type="component" value="Chromosome 7"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="entry name" value="RNase_K"/>
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    <dbReference type="PANTHER" id="PTHR31733">
        <property type="entry name" value="RIBONUCLEASE KAPPA"/>
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    <proteinExistence type="inferred from homology"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00008458"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="99" mass="11042" checksum="061BD5B8CC065661" modified="2012-04-18" version="1">MIKIIGPKCSTCCMLTSVWGIIMMLCLGGAYKLRSPALYKDIPIDFKNAALASNQKEVFAAYDSSGTNCFIAAGLYVLVLFFSLWQWKVNKSLQTYTVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>H2XRD3</accession>
    <name>H2XRD3_CIOIN</name>
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            <fullName evidence="1">Uncharacterized protein</fullName>
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    </protein>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
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                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Wang H.G."/>
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                <person name="Doggett N."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
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                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22677:SF4">
        <property type="entry name" value="USHER SYNDROME TYPE-1G PROTEIN-LIKE PROTEIN"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="373"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="155"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000032490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="420" mass="48476" checksum="7DB821B555764D0C" modified="2012-04-18" version="1">MKSTKPHRPRGGWYSYPGPDECILDMINSQLQRVSEGHKFNVYSLDDLVDSRALCGLVNSFMSGTFTTEVMLNDRWTINLAMRTISKMLHASNPMGSEDLSEADPKSICSYMCFFFMSSYKLRQSMAVLRRLKELTLLVQEVDSEISKFPPKLDSMQHLKLKKQLETNLDELQKEQETLQETYDIEYINDWLAHIKEVQVETRKTIALKMNERFDLVSVPRTLTINELCMSVAINLQLTQGAGFYRGYERETCTPDRRIVLRRKSDQKFLDDFTGVEGSRNIRDILGINKDEVAIVEPAMFPKYEFYFESKSRNKTLRTGATFLYQVFPGNTNQWQRLFFKAVRDGDTDVVKKMVIFFKDSHPDFILSKEIKSGNTALHAAARFGHFEIAKYLLENGCNVNARNTSGWTPLFYAGEGLHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>H2XSJ6</accession>
    <name>H2XSJ6_CIOIN</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01002164">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XSJ6"/>
    <dbReference type="STRING" id="7719.ENSCINP00000032630"/>
    <dbReference type="Ensembl" id="ENSCINT00000031287.1">
        <property type="protein sequence ID" value="ENSCINP00000032630.1"/>
        <property type="gene ID" value="ENSCING00000024542.1"/>
    </dbReference>
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    <dbReference type="HOGENOM" id="CLU_1274767_0_0_1"/>
    <dbReference type="InParanoid" id="H2XSJ6"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23346:SF19">
        <property type="entry name" value="PROTEASOME ADAPTER AND SCAFFOLD PROTEIN ECM29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23346">
        <property type="entry name" value="TRANSLATIONAL ACTIVATOR GCN1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000032630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="217" mass="23604" checksum="8C5F9A57ECBCCEE2" modified="2012-04-18" version="1">MTMLKTEACTSHPAVKLHLMDLQNAFTALLSEPDELTQDIASKGFGAIYDLGSEDQKKMLVDKLVGSLTGSSESKSPASKVRIIGSEGSSLFTGGSVQLGKAPDGSSLNTYKELCSLASDLNQPDLVYKFLHLANHHALWNSKKGAAFGFRSIAIAAREQLTPHLPQIVPRLYRYTFDPQTSVRNAMTSIWNVVAPDTIKTVDEYSSSILEDLLSNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>H2XSR9</accession>
    <accession>A0A1W2WLI6</accession>
    <name>H2XSR9_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transmembrane protein 179-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100185701</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">wild type</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TMEM179 family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01000805">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002130710.1">
        <property type="nucleotide sequence ID" value="XM_002130674.4"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XSR9"/>
    <dbReference type="STRING" id="7719.ENSCINP00000032703"/>
    <dbReference type="Ensembl" id="ENSCINT00000034059.1">
        <property type="protein sequence ID" value="ENSCINP00000032703.1"/>
        <property type="gene ID" value="ENSCING00000022921.1"/>
    </dbReference>
    <dbReference type="GeneID" id="100185701"/>
    <dbReference type="KEGG" id="cin:100185701"/>
    <dbReference type="GeneTree" id="ENSGT00510000048283"/>
    <dbReference type="HOGENOM" id="CLU_103794_0_0_1"/>
    <dbReference type="InParanoid" id="H2XSR9"/>
    <dbReference type="OMA" id="DEFRGHC"/>
    <dbReference type="OrthoDB" id="6423876at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029673">
        <property type="entry name" value="TMEM179"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059010">
        <property type="entry name" value="TMEM179-179B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31872">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31872:SF4">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26158">
        <property type="entry name" value="Claudin_TMEM179-179B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="70"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="106"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="168"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
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        </source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XSX5"/>
    <dbReference type="Ensembl" id="ENSCINT00000037125.1">
        <property type="protein sequence ID" value="ENSCINP00000032759.1"/>
        <property type="gene ID" value="ENSCING00000022289.1"/>
    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="23"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000032759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="49" mass="5762" checksum="B54E85C1BC8B9AB9" modified="2012-04-18" version="1">MKKLPRFTCYTIFPILNKFHNSWIFLQFIADLLGIIKVCTILSICCKII</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>H2XTP7</accession>
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        <recommendedName>
            <fullName evidence="5">Sushi domain-containing protein</fullName>
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    <organism evidence="6 7">
        <name type="scientific">Ciona intestinalis</name>
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        <lineage>
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    <reference evidence="7" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
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                <person name="Kobayashi K."/>
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                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
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                <person name="Richardson P."/>
                <person name="Lucas S."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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            </authorList>
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            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">wild type</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="6" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
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<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
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                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">wild type</strain>
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    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="3" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the SF3B5 family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="Ensembl" id="ENSCINT00000032285.1">
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        <property type="gene ID" value="ENSCING00000021953.1"/>
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    <evidence type="ECO:0000313" key="4">
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    <sequence length="86" mass="10034" checksum="CA07967632A2BDEC" modified="2012-04-18" version="1">MGDRYNIFSQMEHLQSKYVGTGHSDTTKWEWLTNQHRDTLASFVGHHDLLNYIAIAENETKARVRFNMLEKMFQPCGPPPDRPNVD</sequence>
</entry>
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        <name type="scientific">Ciona intestinalis</name>
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    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">wild type</strain>
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    <reference evidence="7" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="7" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="ENSCINP00000033576.1"/>
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        <property type="component" value="Chromosome 9"/>
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    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="entry name" value="von Willebrand factor, type A domain"/>
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        <property type="entry name" value="ECM_Assembly_Org"/>
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        <property type="entry name" value="VWF_A"/>
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        <property type="entry name" value="vWFA_dom_sf"/>
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    <dbReference type="PANTHER" id="PTHR24020">
        <property type="entry name" value="COLLAGEN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24020:SF87">
        <property type="entry name" value="COLLAGEN ALPHA-1(VI) CHAIN-LIKE"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
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        <source>
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        <source>
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    </evidence>
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        <source>
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    <sequence length="240" mass="26885" checksum="BC40DB52BB45D663" modified="2012-04-18" version="1">CNSGKLDLIFLIDESTSVLENDFDGIKVWLRNTISSFPIGEEYTQIGLATYSDNPRIIFHLNKYHKLDDIRKAVLEVEHTSGGTATGKAILYLTNNMFTHENGVRPNAKRLVVVLTDGKSQDDVIVPSRIAKESGIVMFAIGVGKVVMGELRAIASDPDRYVYKINDFSALESIRRELSHSIASLESQGKTSTKEQGQAGELQLLEFQKGVQKMMNFLEKLHRTLQIGFKILQVVRKSRN</sequence>
</entry>
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    <reference evidence="10" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
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                <person name="Tassy O."/>
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                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
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                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
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                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
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                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
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                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
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                <person name="Inaba K."/>
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                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
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                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
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                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
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    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <property type="entry name" value="Nebulin_repeat"/>
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        <property type="entry name" value="LASP"/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Chapman J."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Satou Y."/>
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                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
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                <person name="Goodstein D.M."/>
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        <source>
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        <source>
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    </evidence>
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        <name type="common">Transparent sea squirt</name>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Terry A."/>
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                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
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                <person name="Tokuoka M."/>
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                <person name="Zhang C."/>
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                <person name="Detter C."/>
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                <person name="Richardson P."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="10">wild type</strain>
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    <reference evidence="10" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <location>
            <begin position="31"/>
            <end position="51"/>
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        <location>
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        <location>
            <begin position="218"/>
            <end position="469"/>
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    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000036483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="528" mass="60955" checksum="05D217437C388D02" modified="2012-04-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2005-02-15" modified="2026-01-28" version="147" xmlns="https://uniprot.org/uniprot">
    <accession>Q5IFL5</accession>
    <accession>A0A1W2VNJ2</accession>
    <accession>F7AQT7</accession>
    <name>Q5IFL5_CIOIN</name>
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        <recommendedName>
            <fullName evidence="9">POU domain protein</fullName>
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    </protein>
    <gene>
        <name evidence="13" type="primary">POU-IV</name>
        <name evidence="14" type="synonym">pou-iv</name>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2005" name="Dev. Genes Evol." volume="215" first="41" last="45">
            <title>Ci-POU-IV expression identifies PNS neurons in embryos and larvae of the ascidian Ciona intestinalis.</title>
            <authorList>
                <person name="Candiani S."/>
                <person name="Pennati R."/>
                <person name="Oliveri D."/>
                <person name="Locascio A."/>
                <person name="Branno M."/>
                <person name="Castagnola P."/>
                <person name="Pestarino M."/>
                <person name="De Bernardi F."/>
            </authorList>
            <dbReference type="PubMed" id="15526215"/>
            <dbReference type="DOI" id="10.1007/s00427-004-0444-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">wild type</strain>
        </source>
    </reference>
    <reference evidence="14" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the POU transcription factor family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001421">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="AAW31742.1"/>
        <property type="molecule type" value="mRNA"/>
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    <dbReference type="RefSeq" id="NP_001027972.1">
        <property type="nucleotide sequence ID" value="NM_001032800.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q5IFL5"/>
    <dbReference type="STRING" id="7719.ENSCINP00000010141"/>
    <dbReference type="Ensembl" id="ENSCINT00000010141.3">
        <property type="protein sequence ID" value="ENSCINP00000010141.3"/>
        <property type="gene ID" value="ENSCING00000004922.3"/>
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    <dbReference type="GeneID" id="497238"/>
    <dbReference type="KEGG" id="cin:497238"/>
    <dbReference type="CTD" id="497238"/>
    <dbReference type="GeneTree" id="ENSGT00940000169264"/>
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        <property type="component" value="Chromosome 2"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="POU domain protein"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
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        <property type="entry name" value="HD"/>
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        <property type="entry name" value="Homeobox_CS"/>
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        <property type="entry name" value="Homeodomain-like_sf"/>
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        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
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        <property type="entry name" value="POU"/>
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        <property type="entry name" value="POU_dom"/>
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        <property type="entry name" value="POU_domain_TF"/>
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    <dbReference type="PANTHER" id="PTHR11636:SF70">
        <property type="entry name" value="INHIBITORY POU PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11636">
        <property type="entry name" value="POU DOMAIN"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="POUDOMAIN"/>
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        <property type="entry name" value="HOX"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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    <proteinExistence type="evidence at transcript level"/>
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    <keyword evidence="4 7 8" id="KW-0371">Homeobox</keyword>
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    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="POU-specific" evidence="12">
        <location>
            <begin position="251"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="11">
        <location>
            <begin position="341"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="7">
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    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
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    <evidence type="ECO:0000256" key="9">
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            <dbReference type="RuleBase" id="RU361194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51179"/>
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    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AAW31742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000010141.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="412" mass="45630" checksum="E1AF2033FBC52C41" modified="2005-02-15" version="1">MFTNMLAPHHHHRPPSPPTTMTPLNTSYGEQKYAPLHNAADAVRRHCMASPVAYSGNLFAGFDESFLARQAEALAAVEYPGKSQFKPEPTNHSYYPPAPHYVTQQHTTSAVPPPVYSSMHPPPAFHHSAALQTSLDSSDVIDQLGSSHCGLPTYQPPTFNSSVMMTSDVTSQPHYITHPAPTYNPPNHFHPTSSAMHSTVSVTPISAPIITQAGKYGSGCTTPRDQWDDDVTNVFVTGSPPPSVPSFTSNEYDADPRELEAFAERFKQRRIKLGVTQNDVGQALANLKLPGVGSLSQSTICRFESLTLSHNNMVALKPILITWLEKAEEEHRRKMENAAMGEKKRKRTSIAAPEKRSLEAYFAVQPRPSSEKIAAIAEKLDLKKNVVRVWFCNQRQKQKRMKFSAFNGDVIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>F6PM82</accession>
    <accession>A0A1W2W8F8</accession>
    <name>F6PM82_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Hippocalcin-like protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100184110</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">wild type</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the recoverin family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002766">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_026691275.1">
        <property type="nucleotide sequence ID" value="XM_026835474.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6PM82"/>
    <dbReference type="FunCoup" id="F6PM82">
        <property type="interactions" value="34"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000025090"/>
    <dbReference type="Ensembl" id="ENSCINT00000025336.2">
        <property type="protein sequence ID" value="ENSCINP00000025090.2"/>
        <property type="gene ID" value="ENSCING00000003215.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100184110"/>
    <dbReference type="KEGG" id="cin:100184110"/>
    <dbReference type="GeneTree" id="ENSGT00940000163010"/>
    <dbReference type="HOGENOM" id="CLU_072366_1_0_1"/>
    <dbReference type="InParanoid" id="F6PM82"/>
    <dbReference type="OMA" id="QMYDPAR"/>
    <dbReference type="OrthoDB" id="191686at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000009">
        <property type="entry name" value="Visinin-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028846">
        <property type="entry name" value="Recoverin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23055">
        <property type="entry name" value="CALCIUM BINDING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23055:SF178">
        <property type="entry name" value="NEUROCALCIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13833">
        <property type="entry name" value="EF-hand_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00450">
        <property type="entry name" value="RECOVERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0519">Myristate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="7">
        <location>
            <begin position="60"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="7">
        <location>
            <begin position="96"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="7">
        <location>
            <begin position="144"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000025090.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="194" mass="21991" checksum="19DE955CCA9E38C7" modified="2012-04-18" version="2">MGKQNSKLKPEDIRDLRAATEFNEHELQEWYKGFIKDCPSGNLTMDEFQKIYANFFPQGDASKFAAHVFRTFDSNGDSTIDFREFIVALSVTSRGNLEEKLKWAFSMYDCDGNGIISRDEMLEIVRAIYKMVGAVMKMPEDESTPEKRTDKIFKQMDKNLDGSISLEEFVEGAKKDPSIVRLLQCTPGSGGLGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>F6PVI5</accession>
    <name>F6PVI5_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">1,4-alpha-glucan branching enzyme</fullName>
            <ecNumber evidence="3">2.4.1.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100181012</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Transfers a segment of a (1-&gt;4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.</text>
            <dbReference type="EC" id="2.4.1.18"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.18" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="F6PVI5"/>
    <dbReference type="STRING" id="7719.ENSCINP00000001394"/>
    <dbReference type="Ensembl" id="ENSCINT00000001394.3">
        <property type="protein sequence ID" value="ENSCINP00000001394.3"/>
        <property type="gene ID" value="ENSCING00000000748.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000017040"/>
    <dbReference type="HOGENOM" id="CLU_011131_2_2_1"/>
    <dbReference type="InParanoid" id="F6PVI5"/>
    <dbReference type="OMA" id="TPWAHTR"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003844">
        <property type="term" value="F:1,4-alpha-glucan branching enzyme activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043169">
        <property type="term" value="F:cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005978">
        <property type="term" value="P:glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd11321">
        <property type="entry name" value="AmyAc_bac_euk_BE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02854">
        <property type="entry name" value="E_set_GBE_euk_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002893">
        <property type="entry name" value="1,4-alpha-glucan-branching enzyme-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1180:FF:000197">
        <property type="entry name" value="Glucan (1,4-alpha-),-branching enzyme 1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006048">
        <property type="entry name" value="A-amylase/branching_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037439">
        <property type="entry name" value="Branching_enzy"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="GH13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004193">
        <property type="entry name" value="Glyco_hydro_13_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43651">
        <property type="entry name" value="1,4-ALPHA-GLUCAN-BRANCHING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43651:SF3">
        <property type="entry name" value="1,4-ALPHA-GLUCAN-BRANCHING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02806">
        <property type="entry name" value="Alpha-amylase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02922">
        <property type="entry name" value="CBM_48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000463">
        <property type="entry name" value="GlgB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyl hydrolase family 13 catalytic" evidence="6">
        <location>
            <begin position="192"/>
            <end position="549"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000001394.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="649" mass="76225" checksum="962EE764F33E78D0" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>F6Q0H1</accession>
    <name>F6Q0H1_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">wild type</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001088">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6Q0H1"/>
    <dbReference type="STRING" id="7719.ENSCINP00000009539"/>
    <dbReference type="Ensembl" id="ENSCINT00000009539.3">
        <property type="protein sequence ID" value="ENSCINP00000009539.3"/>
        <property type="gene ID" value="ENSCING00000004617.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160615"/>
    <dbReference type="HOGENOM" id="CLU_004826_12_0_1"/>
    <dbReference type="InParanoid" id="F6Q0H1"/>
    <dbReference type="OMA" id="QNCANDV"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120025">
        <property type="term" value="C:plasma membrane bounded cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048589">
        <property type="term" value="P:developmental growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051241">
        <property type="term" value="P:negative regulation of multicellular organismal process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050877">
        <property type="term" value="P:nervous system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051240">
        <property type="term" value="P:positive regulation of multicellular organismal process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000247">
        <property type="entry name" value="Delta/notch like EGF repeat containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000508">
        <property type="entry name" value="Eyes shut homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000066">
        <property type="entry name" value="FAT atypical cadherin 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000029">
        <property type="entry name" value="neurexin-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000004">
        <property type="entry name" value="Neurogenic locus notch 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000031">
        <property type="entry name" value="neurogenic locus notch homolog protein 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000327">
        <property type="entry name" value="neurogenic locus notch homolog protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Notch homolog 4, [Drosophila]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000434">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000143">
        <property type="entry name" value="Protein crumbs 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000122">
        <property type="entry name" value="Protein crumbs homolog 2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013032">
        <property type="entry name" value="EGF-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051022">
        <property type="entry name" value="Notch_Cell-Fate_Det"/>
    </dbReference>
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        <property type="entry name" value="CRUMBS FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24049:SF22">
        <property type="entry name" value="DROSOPHILA CRUMBS HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="4"/>
    </dbReference>
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        <property type="entry name" value="EGFBLOOD"/>
    </dbReference>
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        <property type="match status" value="17"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
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        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="9 11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2 11" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="4"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="43"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="81"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="118"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="156"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="230"/>
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        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="270"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="308"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="346"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="384"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="422"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="461"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="500"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="538"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="577"/>
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        </location>
    </feature>
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        <location>
            <begin position="69"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="107"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="144"/>
            <end position="153"/>
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    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
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    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
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    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="334"/>
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    <feature type="disulfide bond" evidence="11">
        <location>
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    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
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            <end position="419"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
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        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="526"/>
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        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="565"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="608"/>
            <end position="617"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
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                <person name="Roe B.A."/>
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                <person name="Lindquist E."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">wild type</strain>
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    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="4">Microtubule inner protein (MIP) part of the dynein-decorated doublet microtubules (DMTs) in cilia axoneme. Acts as a regulator of cilium basal body docking and positioning in mono- and multiciliated cells. Regulates basal body docking and cilia formation in multiciliated lung cells. Regulates kinocilium positioning and stereocilia bundle morphogenesis in the inner ear.</text>
    </comment>
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        <text evidence="1">Belongs to the Flattop family.</text>
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        <property type="gene ID" value="ENSCING00000009051.3"/>
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    <dbReference type="KEGG" id="cin:100185443"/>
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        <property type="component" value="Chromosome 10"/>
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        <location>
            <begin position="46"/>
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        </location>
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    </feature>
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        <location>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000018371.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    <dbReference type="STRING" id="7719.ENSCINP00000020668"/>
    <dbReference type="Ensembl" id="ENSCINT00000020668.3">
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        <property type="gene ID" value="ENSCING00000014810.2"/>
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    <dbReference type="InParanoid" id="F6R6Z5"/>
    <dbReference type="OMA" id="EMIDVEW"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18186">
        <property type="entry name" value="BTB_POZ_ZBTB_KLHL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
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    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
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        <property type="entry name" value="LD33804P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="1">
        <location>
            <begin position="33"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000020668.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    </evidence>
    <sequence length="117" mass="13626" checksum="30151AE24A91A2F5" modified="2012-04-18" version="2">MASKEETVIQYKSNHEGRVLTKLNRLRKVKMFTDVTFKLEDGEVSAHRNVLAAYSDYYEKMFTSGFKEGFNKDIEIKEIKASILDLLFTFIYLQVIDISDKTVCQLYEASDYLQFNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
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    <name>F6RE26_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Fibrinogen C-terminal domain-containing protein</fullName>
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    </protein>
    <organism evidence="9 10">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
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            <taxon>Tunicata</taxon>
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            <taxon>Ciona</taxon>
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    <reference evidence="10" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
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                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">wild type</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="EAAA01000072">
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="Ensembl" id="ENSCINT00000025419.2">
        <property type="protein sequence ID" value="ENSCINP00000025173.2"/>
        <property type="gene ID" value="ENSCING00000004800.3"/>
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    <dbReference type="GeneTree" id="ENSGT00940000173734"/>
    <dbReference type="HOGENOM" id="CLU_574349_0_0_1"/>
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    <dbReference type="OMA" id="CCERVSF"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 1"/>
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    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="FIB_ANG-like"/>
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    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040941">
        <property type="entry name" value="GGGWT_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47221">
        <property type="entry name" value="FIBRINOGEN ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47221:SF6">
        <property type="entry name" value="FIBRINOGEN ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="8">
        <location>
            <begin position="412"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="239"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="291"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="324"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="246"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="296"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="332"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000025173.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="476" mass="52685" checksum="0169C81EA4F09B22" modified="2012-04-18" version="2">MLSIPIYILSILTLQSPFNHLLVNGDPTSPLATNDAAANEAASCAMRVGRNCDDDGSCRYKVNVPSFDIVGEFAQMFSANRGGGNFAAECSRLGMNLEIIRDNQAAWVRRSQGLLSNVTKDLTRLEVGFTEKMTALSNSVRVLETKTTQTLTAINRTMISAANDHESDNEDEDSIALLVRMGKLTTPRPKSRIEVERNEQKQSNFEQRILELQESLQQTLKGMESKLIARIEQISAACSGGRTSDTVDDQPDTEEALSTPAVDEGAVESSKGPQMMLCRTCCERVSFAEYTRSQENDDDAEEAEEDVTRTVETTDRVTGVEQVYTEDETQETRSTTRSQVTSSRSDVTHENNDAMFTSTTVTHAVEVTQPNYIYSEGSGVNIRDGYATNETEGMEFTSRILRATNPTPVQDGQTESLPYDCAELYARGVRQSGVYDIRPGTKVTWTVYCDMDTDGGGWTMLQRRIDGIVNFSKGWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>F6RMX1</accession>
    <name>F6RMX1_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="F6RMX1"/>
    <dbReference type="Ensembl" id="ENSCINT00000011935.3">
        <property type="protein sequence ID" value="ENSCINP00000011935.3"/>
        <property type="gene ID" value="ENSCING00000005788.3"/>
    </dbReference>
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    <dbReference type="HOGENOM" id="CLU_1450753_0_0_1"/>
    <dbReference type="InParanoid" id="F6RMX1"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053103">
        <property type="entry name" value="IDLSRF-like_peptide"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20967">
        <property type="entry name" value="PROHORMONE-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20967:SF0">
        <property type="entry name" value="PROHORMONE-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00261">
        <property type="entry name" value="LDLRECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
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        <location>
            <begin position="22"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="157"/>
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        </location>
    </feature>
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        <location>
            <begin position="136"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000011935.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="187" mass="20447" checksum="0502D80ECB8429A6" modified="2012-04-18" version="2">MHCTGLPIFILLSLSLQVVLAGTAYDYTDDYNSDYVDDYKLEENDTPSYICPGEHIYIEISLLCDGFEDCLDGMDENVEICKIYGRTTTESPSPMIHTAPPTTADPLDYFDYGNCEAGTYQCPSYPGLCLVSETLCDGRADCPGGEDEMNCHERLSTTNSAPTESNDVTTVAKQPTNTTLESSSAPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>F6S161</accession>
    <name>F6S161_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">G-patch domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
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            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
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            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="F6S161"/>
    <dbReference type="Ensembl" id="ENSCINT00000006864.3">
        <property type="protein sequence ID" value="ENSCINP00000006864.3"/>
        <property type="gene ID" value="ENSCING00000024605.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000004191"/>
    <dbReference type="HOGENOM" id="CLU_1229545_0_0_1"/>
    <dbReference type="InParanoid" id="F6S161"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039536">
        <property type="term" value="P:negative regulation of RIG-I signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032480">
        <property type="term" value="P:negative regulation of type I interferon production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000467">
        <property type="entry name" value="G_patch_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040341">
        <property type="entry name" value="GPATCH3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14390">
        <property type="entry name" value="G PATCH DOMAIN CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14390:SF2">
        <property type="entry name" value="G PATCH DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00443">
        <property type="entry name" value="G_patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50174">
        <property type="entry name" value="G_PATCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G-patch" evidence="2">
        <location>
            <begin position="34"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="160"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="170"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="203"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000006864.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="225" mass="25149" checksum="AA5B4B5CD077248A" modified="2012-04-18" version="2">MRKEKRLREGAALKSGLKLSKTKVKKMGKFEEHTRGFGRHIMEGLGWNDDVGLGRGGRRDPVEANGQVSRDGLGFKEPYRPAHRSGEPLASSSHFFPQFRSNISRISKNRRFSGRNRKQAVPGTDGIVITTIYDEPLDVDLEPGLLRSAHSGAMKRRRCLSPTIVPEPTPATTSSSISKLVNPPTRYTEELNEPPQDIKRTKQTQSLSSPMNNINLIKFVKSSDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>F6S7U7</accession>
    <name>F6S7U7_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glutamate dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">wild type</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-glutamate + NAD(+) + H2O = 2-oxoglutarate + NH4(+) + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15133"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.4.1.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-glutamate + NADP(+) + H2O = 2-oxoglutarate + NH4(+) + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11612"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.4.1.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7 11">Belongs to the Glu/Leu/Phe/Val dehydrogenases family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001285">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6S7U7"/>
    <dbReference type="FunCoup" id="F6S7U7">
        <property type="interactions" value="245"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000014974"/>
    <dbReference type="Ensembl" id="ENSCINT00000014974.3">
        <property type="protein sequence ID" value="ENSCINP00000014974.3"/>
        <property type="gene ID" value="ENSCING00000007260.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000000854"/>
    <dbReference type="HOGENOM" id="CLU_025763_1_0_1"/>
    <dbReference type="InParanoid" id="F6S7U7"/>
    <dbReference type="OMA" id="MIMGWMM"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004352">
        <property type="term" value="F:glutamate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006538">
        <property type="term" value="P:L-glutamate catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01076">
        <property type="entry name" value="NAD_bind_1_Glu_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10860:FF:000007">
        <property type="entry name" value="Glutamate dehydrogenase 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000100">
        <property type="entry name" value="Glutamate dehydrogenase 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10860">
        <property type="entry name" value="Leucine Dehydrogenase, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006095">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006096">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006097">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033524">
        <property type="entry name" value="Glu/Leu/Phe/Val_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014362">
        <property type="entry name" value="Glu_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033922">
        <property type="entry name" value="NAD_bind_Glu_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11606">
        <property type="entry name" value="GLUTAMATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11606:SF13">
        <property type="entry name" value="GLUTAMATE DEHYDROGENASE 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00208">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02812">
        <property type="entry name" value="ELFV_dehydrog_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000185">
        <property type="entry name" value="Glu_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00082">
        <property type="entry name" value="GLFDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00839">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00074">
        <property type="entry name" value="GLFV_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0520">NAD</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 7 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamate/phenylalanine/leucine/valine/L-tryptophan dehydrogenase C-terminal" evidence="12">
        <location>
            <begin position="208"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="8">
        <location>
            <position position="126"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="254"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="385"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Important for catalysis" evidence="10">
        <location>
            <position position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000185-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000185-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000185-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000014974.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="505" mass="56054" checksum="5DDA41934A9F5FE3" modified="2012-04-18" version="2">ASADEDDDPNFYHMVGNFFEKGARLVEDKLVEQLNEPADDQRKRVQGIFKIMRPCNHVLQVTFPLKRDNGEHEIITGYRAQHSQHRMPTKGGMRYAPDVNIDEVKALASLMTWKCAVVDVPFGGAKAGVKIDPKLYSERELEKITRRFAMELAKKGFLGPGTDVPAPDVNTGAREMSWMADTYAMTYGHGDINAHACVTGKPITQGGIHGRVSATGRGVFHGIENFINEATFMSAVGMTPGFQDKTFIIQGFGNVGLHSMRYLHSSTCVRCCIGVIEWDGCIYNQEGIDPIELEDYKVANGTIVGFPGAQPYEDETPMLEVDCDILLPCAREKVITAENAPRIKARIIAEGANGPTTPAADRILQANDKLVIPDLYLNAGGVTVSYFEWLKNLNHVSYGRLTFKYEREANYDLLDSVQSSLERKFGKYGGQIPVTPTEEFSKKIHGASEKDIVHSGLSYTMERSARQIIHTARSYELGLDLRTAAYVNAIDKVFKVYNEAGFTFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>F6SKP5</accession>
    <name>F6SKP5_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">5'-Nucleotidase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the 5'-nucleotidase family.</text>
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    <dbReference type="AlphaFoldDB" id="F6SKP5"/>
    <dbReference type="STRING" id="7719.ENSCINP00000008305"/>
    <dbReference type="Ensembl" id="ENSCINT00000008305.3">
        <property type="protein sequence ID" value="ENSCINP00000008305.3"/>
        <property type="gene ID" value="ENSCING00000023290.1"/>
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    <dbReference type="GeneTree" id="ENSGT00530000063775"/>
    <dbReference type="HOGENOM" id="CLU_005854_7_2_1"/>
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    <dbReference type="OMA" id="HTHDGVP"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
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        <property type="project" value="InterPro"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="5'-Nucleotidase, C-terminal domain"/>
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        <property type="entry name" value="5'-Nucleotdase_C_sf"/>
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    <dbReference type="InterPro" id="IPR006179">
        <property type="entry name" value="5_nucleotidase/apyrase"/>
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    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
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        <property type="entry name" value="CG11883_N"/>
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    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
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        <property type="entry name" value="5'-NUCLEOTIDASE"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR11575">
        <property type="entry name" value="5'-NUCLEOTIDASE-RELATED"/>
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        <property type="entry name" value="5_nucleotid_C"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
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        <property type="entry name" value="APYRASEFAMLY"/>
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        <property type="entry name" value="5'-nucleotidase (syn. UDP-sugar hydrolase), C-terminal domain"/>
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    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="4">
        <location>
            <begin position="2"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="5'-Nucleotidase C-terminal" evidence="5">
        <location>
            <begin position="294"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU362119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000008305.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="462" mass="51420" checksum="6487D01BEBD82745" modified="2012-04-18" version="2">LSILHFNDVYEITERKKEPVGGAARFASLMKRNIYDITKQTGERPLVVFSGDCLNPSTLSCATQGRHMIEVLDTLEVDVAVFGNHEFDFGVEHAAKCIERMTSTWLLGNVIDKLTQQNLGGGMTKHIIKRNNGLKIGLLGLVEEEWLDTLATVNREDLEFQDYVERGREVAAELRADGADLVIALTHMRWPNDIRLARMVPDIDIILGGHDHEYGVKEIDECLIIKSGSDFRNLSSVDLFIRNDGCVDVKISRYDVTREVEEDPYMLKIVERYTSVLEKSLDHCIGVVNTSLDGRFKMVRSRETNLGNFVADIMASVTRADVVLINSGTFRSDGVHSCGNFRLHDLMTILPINDCVVTIVVTGKQLLEALENGVSLVPKYEGRFPQVSGVTFGFNPGAPPGSRIVRDTLMVQGNPLDPTREYTMATKAYVARGKDGYTCLSKCRILTDPDSGPLLSTVVRNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>F6SMZ6</accession>
    <name>F6SMZ6_CIOIN</name>
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        <recommendedName>
            <fullName evidence="8">Sorting nexin-14</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">wild type</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sorting nexin family.</text>
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    <dbReference type="EMBL" id="EAAA01001620">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="F6SMZ6"/>
    <dbReference type="FunCoup" id="F6SMZ6">
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    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000022350"/>
    <dbReference type="Ensembl" id="ENSCINT00000022596.2">
        <property type="protein sequence ID" value="ENSCINP00000022350.2"/>
        <property type="gene ID" value="ENSCING00000011780.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182856"/>
    <dbReference type="HOGENOM" id="CLU_014115_0_0_1"/>
    <dbReference type="InParanoid" id="F6SMZ6"/>
    <dbReference type="OMA" id="MYVYVIS"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080025">
        <property type="term" value="F:phosphatidylinositol-3,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097352">
        <property type="term" value="P:autophagosome maturation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08722">
        <property type="entry name" value="RGS_SNX14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="1.10.167.10">
        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR003114">
        <property type="entry name" value="Phox_assoc"/>
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    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
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        <property type="entry name" value="RGS"/>
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        <property type="entry name" value="RGS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044926">
        <property type="entry name" value="RGS_subdomain_2"/>
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    <dbReference type="InterPro" id="IPR037436">
        <property type="entry name" value="SNX14_PX"/>
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    <dbReference type="InterPro" id="IPR037892">
        <property type="entry name" value="SNX14_RGS"/>
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        <property type="entry name" value="Sorting_nexin_C"/>
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        <property type="entry name" value="SORTING NEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22775:SF44">
        <property type="entry name" value="SORTING NEXIN-14"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Nexin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02194">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00615">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00313">
        <property type="entry name" value="PXA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF48097">
        <property type="entry name" value="Regulator of G-protein signaling, RGS"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="PXA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50132">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013288511" description="Sorting nexin-14" evidence="2">
        <location>
            <begin position="16"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="domain" description="PXA" evidence="5">
        <location>
            <begin position="79"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="RGS" evidence="3">
        <location>
            <begin position="285"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="PX" evidence="4">
        <location>
            <begin position="516"/>
            <end position="635"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51207"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000022350.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="892" mass="104174" checksum="BEAC87705D237170" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>F6T283</accession>
    <name>F6T283_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit gamma</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">wild type</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="Ensembl" id="ENSCINT00000017578.3">
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        <property type="gene ID" value="ENSCING00000008618.3"/>
    </dbReference>
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    <dbReference type="HOGENOM" id="CLU_035365_1_0_1"/>
    <dbReference type="InParanoid" id="F6T283"/>
    <dbReference type="OMA" id="HKFWAYE"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
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        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
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    </dbReference>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:T cell homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="entry name" value="Serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit gamma"/>
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        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
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        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
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        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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        <source>
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    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    </evidence>
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        <name type="scientific">Ciona intestinalis</name>
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    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
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            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">wild type</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="7" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0000318"/>
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    <dbReference type="GO" id="GO:0030833">
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        <property type="project" value="GO_Central"/>
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        <property type="entry name" value="CORTACTIN AND DREBRIN"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR10829:SF23">
        <property type="entry name" value="CORTACTIN, ISOFORM A"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF02218">
        <property type="entry name" value="HS1_rep"/>
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    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
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        <property type="entry name" value="SH3"/>
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    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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        </location>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="242"/>
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        </location>
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        <location>
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    </feature>
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        <location>
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    </feature>
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        <location>
            <begin position="464"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
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        <source>
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        <source>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50002"/>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000017293.3"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="573" mass="64990" checksum="F487AD52CFD6705C" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>F6TD87</accession>
    <name>F6TD87_CIOIN</name>
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        <recommendedName>
            <fullName evidence="8">Kringle domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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            <taxon>Eukaryota</taxon>
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    <reference evidence="7" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="F6TD87"/>
    <dbReference type="STRING" id="7719.ENSCINP00000025646"/>
    <dbReference type="Ensembl" id="ENSCINT00000025892.2">
        <property type="protein sequence ID" value="ENSCINP00000025646.2"/>
        <property type="gene ID" value="ENSCING00000014101.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000175008"/>
    <dbReference type="HOGENOM" id="CLU_1630780_0_0_1"/>
    <dbReference type="InParanoid" id="F6TD87"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00108">
        <property type="entry name" value="KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.20.10:FF:000025">
        <property type="entry name" value="Plasminogen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.20.10">
        <property type="entry name" value="Plasminogen Kringle 4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FA58C"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR050759">
        <property type="entry name" value="Serine_protease_kringle"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24261:SF7">
        <property type="entry name" value="KRINGLE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24261">
        <property type="entry name" value="PLASMINOGEN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Kringle"/>
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        <property type="entry name" value="KRINGLE"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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    </dbReference>
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    <keyword evidence="1 3" id="KW-0420">Kringle</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="41"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="F5/8 type C" evidence="4">
        <location>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50070"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000025646.2"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="163" mass="18241" checksum="58A4D92E8323F713" modified="2012-04-18" version="2">MNSGYIQDNMISASSFAQGKEAKQARPPVDKAECLANAKDDGESYRGWISKTQDGTVCQKWSSQTPHSHDRTPQNYPDSGLVSNFCRNPDGEKGPWCYTTDPNVRWTYCTVPVCPSYVAAGWTPLTVNAAQWVQVQFDSYYKVSGITMYGNTATYVSQYVFQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>F6TJ71</accession>
    <name>F6TJ71_CIOIN</name>
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        <recommendedName>
            <fullName evidence="15">tRNA-t(6)A37 methylthiotransferase</fullName>
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        </recommendedName>
    </protein>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">wild type</strain>
        </source>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 15">Catalyzes the methylthiolation of N6-threonylcarbamoyladenosine (t(6)A), leading to the formation of 2-methylthio-N6-threonylcarbamoyladenosine (ms(2)t(6)A) at position 37 in tRNAs that read codons beginning with adenine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 15">
            <text>N(6)-L-threonylcarbamoyladenosine(37) in tRNA + (sulfur carrier)-SH + AH2 + 2 S-adenosyl-L-methionine = 2-methylsulfanyl-N(6)-L-threonylcarbamoyladenosine(37) in tRNA + (sulfur carrier)-H + 5'-deoxyadenosine + L-methionine + A + S-adenosyl-L-homocysteine + 2 H(+)</text>
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            <dbReference type="Rhea" id="RHEA-COMP:10163"/>
            <dbReference type="Rhea" id="RHEA-COMP:11092"/>
            <dbReference type="Rhea" id="RHEA-COMP:14737"/>
            <dbReference type="Rhea" id="RHEA-COMP:14739"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:29917"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64428"/>
            <dbReference type="ChEBI" id="CHEBI:74418"/>
            <dbReference type="ChEBI" id="CHEBI:74420"/>
            <dbReference type="EC" id="2.8.4.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="15">Binds 1 or 2 [4Fe-4S] cluster. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="15">Endoplasmic reticulum membrane</location>
            <topology evidence="15">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the methylthiotransferase family. CDKAL1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.8.4.5" evidence="15"/>
    <dbReference type="EMBL" id="EAAA01001711">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="F6TJ71"/>
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        <property type="interactions" value="474"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000019474"/>
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        <property type="protein sequence ID" value="ENSCINP00000019474.3"/>
        <property type="gene ID" value="ENSCING00000009576.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155952"/>
    <dbReference type="HOGENOM" id="CLU_018697_4_1_1"/>
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    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
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        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
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        <property type="term" value="C:endoplasmic reticulum membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
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    <dbReference type="GO" id="GO:0035598">
        <property type="term" value="F:tRNA (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-methylthiotransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
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    <dbReference type="GO" id="GO:0035600">
        <property type="term" value="P:tRNA methylthiolation"/>
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        <property type="entry name" value="Radical_SAM"/>
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    <dbReference type="FunFam" id="3.40.50.12160:FF:000005">
        <property type="entry name" value="threonylcarbamoyladenosine tRNA methylthiotransferase isoform X1"/>
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        <property type="entry name" value="threonylcarbamoyladenosine tRNA methylthiotransferase isoform X2"/>
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    <dbReference type="Gene3D" id="3.40.50.12160">
        <property type="entry name" value="Methylthiotransferase, N-terminal domain"/>
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        <property type="entry name" value="tm_1862 like domain"/>
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        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
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        <property type="entry name" value="Methylthiotransferase"/>
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        <property type="entry name" value="Methylthiotransferase_CS"/>
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        <property type="entry name" value="Methylthiotransferase_N_sf"/>
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    <dbReference type="InterPro" id="IPR006466">
        <property type="entry name" value="MiaB-like_arc_euk"/>
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    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
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        <property type="entry name" value="rSAM_horseshoe"/>
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    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
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    <dbReference type="InterPro" id="IPR002792">
        <property type="entry name" value="TRAM_dom"/>
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        <property type="entry name" value="MiaB-like-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00089">
        <property type="entry name" value="MiaB/RimO family radical SAM methylthiotransferase"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR11918">
        <property type="entry name" value="RADICAL SAM PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11918:SF45">
        <property type="entry name" value="THREONYLCARBAMOYLADENOSINE TRNA METHYLTHIOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
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    </dbReference>
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        <property type="entry name" value="UPF0004"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01082">
        <property type="entry name" value="B12-binding_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51449">
        <property type="entry name" value="MTTASE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01278">
        <property type="entry name" value="MTTASE_RADICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50926">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 15" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="15" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11 15" id="KW-0408">Iron</keyword>
    <keyword evidence="12 15" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="13 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 15" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8 15" id="KW-0819">tRNA processing</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="533"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="domain" description="MTTase N-terminal" evidence="17">
        <location>
            <begin position="64"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="18">
        <location>
            <begin position="201"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="domain" description="TRAM" evidence="16">
        <location>
            <begin position="432"/>
            <end position="494"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002399"/>
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    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00008616"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00051661"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
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        <source>
            <dbReference type="PROSITE" id="PS51449"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51918"/>
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    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000019474.3"/>
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    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="558" mass="62861" checksum="7D1BBD5789215951" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>F6U2V6</accession>
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        <submittedName>
            <fullName evidence="6">Adenylate kinase 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC100183643</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <dbReference type="OMA" id="HAYVITP"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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    <dbReference type="GO" id="GO:0005737">
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        <property type="project" value="GO_Central"/>
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        <property type="term" value="F:AMP kinase activity"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="cAMP-dependent protein kinase regulatory subunit, dimerization-anchoring domain"/>
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        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
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    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
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        <property type="entry name" value="NUCLEOTIDE KINASE"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Chapman J."/>
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                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
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                <person name="Hastings K.E."/>
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                <person name="Huang W."/>
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                <person name="Martinez D."/>
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                <person name="Putnam N."/>
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                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Sasakura Y."/>
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                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
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                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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                <person name="Meinertzhagen I.A."/>
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                <person name="Putnam N."/>
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                <person name="Terry A."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">wild type</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01002028">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="F6UEA0"/>
    <dbReference type="FunCoup" id="F6UEA0">
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    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000024696"/>
    <dbReference type="Ensembl" id="ENSCINT00000024942.2">
        <property type="protein sequence ID" value="ENSCINP00000024696.2"/>
        <property type="gene ID" value="ENSCING00000013461.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00550000075207"/>
    <dbReference type="HOGENOM" id="CLU_1242785_0_0_1"/>
    <dbReference type="InParanoid" id="F6UEA0"/>
    <dbReference type="OMA" id="CRSDLDM"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008106">
        <property type="term" value="F:alcohol dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
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        <property type="term" value="F:zinc ion binding"/>
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    <dbReference type="GO" id="GO:0006066">
        <property type="term" value="P:alcohol metabolic process"/>
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    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
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        <property type="entry name" value="ADH-like_N"/>
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    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
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    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
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        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
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        <property type="entry name" value="Zn_alcohol_dh"/>
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        <property type="entry name" value="D-ARABINOSE 1-DEHYDROGENASE (NADP(+))"/>
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        <property type="entry name" value="ADH_ZINC"/>
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    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alcohol dehydrogenase-like N-terminal" evidence="5">
        <location>
            <begin position="26"/>
            <end position="150"/>
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        <location>
            <begin position="230"/>
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        <location>
            <begin position="244"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000024696.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="254" mass="27035" checksum="3D66D2734CE2CCEB" modified="2012-04-18" version="2">MKKAEIVEAKKPLKISEAPVPKPPKDGAVVKLLYSGICHSDIHQIDNGFDVGSRFTCLTDDPNYQFPIVPGHEMAGVVHSLGENAKPCDLKIGDRVAVFPWIGCGKCGYCDNGYSQFCKEKHHTMGFGAVPGGYSNYTVVIHHQFLVKVPASIPLSIATMLPCSGLTTYNAVTTALPAIKKATAFKGKASMLIIGAGGLGLWALQLAKNILPKTTNVVVADLSESKLGNAKERGADGTVAWDPSKSEEDLIKAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>F6UXC1</accession>
    <accession>A0A1W2WB86</accession>
    <name>F6UXC1_CIOIN</name>
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        <submittedName>
            <fullName evidence="5">Synaptonemal complex protein 3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC100187231</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">wild type</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the XLR/SYCP3 family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001693">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="EAAA01001694">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002125203.2">
        <property type="nucleotide sequence ID" value="XM_002125167.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6UXC1"/>
    <dbReference type="FunCoup" id="F6UXC1">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000019408"/>
    <dbReference type="Ensembl" id="ENSCINT00000019408.3">
        <property type="protein sequence ID" value="ENSCINP00000019408.3"/>
        <property type="gene ID" value="ENSCING00000009534.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100187231"/>
    <dbReference type="KEGG" id="cin:100187231"/>
    <dbReference type="GeneTree" id="ENSGT00390000000062"/>
    <dbReference type="HOGENOM" id="CLU_101820_2_0_1"/>
    <dbReference type="InParanoid" id="F6UXC1"/>
    <dbReference type="OMA" id="MATHKGC"/>
    <dbReference type="OrthoDB" id="9621324at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000795">
        <property type="term" value="C:synaptonemal complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007286">
        <property type="term" value="P:spermatid development"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051443">
        <property type="entry name" value="XLR/SYCP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006888">
        <property type="entry name" value="XLR/SYCP3/FAM9_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19368">
        <property type="entry name" value="XLR/SCP3/FAM9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19368:SF15">
        <property type="entry name" value="XLR_SYCP3_FAM9 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04803">
        <property type="entry name" value="Cor1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="XLR/SYCP3/FAM9" evidence="4">
        <location>
            <begin position="77"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="97"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="51"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000019408.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="230" mass="27088" checksum="D45686BC24E9D93A" modified="2011-07-27" version="1">MPRKSKESKPTFEYEQDFMEGMESGSEPKEAIICEVKQPKKPKKRSSVEYEREESDYEEDEQPVASILNQFGSDIKKSISSKRRKLEAFTKQCCAAREKLDSVLENQKKDRSTLNEEYKKQISSVINQWETDIDKAKENEEKLNNLLTQQMKLITQVRVVQTQRFKSIKQLHDQYIKSVGTVNETHSQQHKDVNKEIDKELSNLQKKYLLESRNQEMATMRKSLHSMLYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>F6UYN1</accession>
    <name>F6UYN1_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">KAP NTPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="F6UYN1"/>
    <dbReference type="STRING" id="7719.ENSCINP00000026590"/>
    <dbReference type="Ensembl" id="ENSCINT00000026836.2">
        <property type="protein sequence ID" value="ENSCINP00000026590.2"/>
        <property type="gene ID" value="ENSCING00000014800.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00650000093443"/>
    <dbReference type="HOGENOM" id="CLU_460743_0_0_1"/>
    <dbReference type="InParanoid" id="F6UYN1"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011646">
        <property type="entry name" value="KAP_P-loop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052754">
        <property type="entry name" value="NTPase_KAP_P-loop"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22674:SF6">
        <property type="entry name" value="NTPASE KAP FAMILY P-LOOP DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22674">
        <property type="entry name" value="NTPASE, KAP FAMILY P-LOOP DOMAIN-CONTAINING 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07693">
        <property type="entry name" value="KAP_NTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="KAP NTPase" evidence="2">
        <location>
            <begin position="139"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000026590.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="592" mass="67020" checksum="9F709B8F596AB86F" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>F6VBR9</accession>
    <name>F6VBR9_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphatidylinositol-specific phospholipase C X domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="F6VBR9"/>
    <dbReference type="STRING" id="7719.ENSCINP00000003183"/>
    <dbReference type="Ensembl" id="ENSCINT00000003183.3">
        <property type="protein sequence ID" value="ENSCINP00000003183.3"/>
        <property type="gene ID" value="ENSCING00000001592.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000170995"/>
    <dbReference type="HOGENOM" id="CLU_051926_0_0_1"/>
    <dbReference type="InParanoid" id="F6VBR9"/>
    <dbReference type="OMA" id="WANTQSC"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08616">
        <property type="entry name" value="PI-PLCXD1c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051057">
        <property type="entry name" value="PI-PLC_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042158">
        <property type="entry name" value="PLCXD1/2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000909">
        <property type="entry name" value="PLipase_C_PInositol-sp_X_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13593">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13593:SF113">
        <property type="entry name" value="SI:DKEY-266F7.9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00388">
        <property type="entry name" value="PI-PLC-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00148">
        <property type="entry name" value="PLCXc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50007">
        <property type="entry name" value="PIPLC_X_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphatidylinositol-specific phospholipase C X" evidence="1">
        <location>
            <begin position="9"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000003183.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="282" mass="32049" checksum="A07ED0C3AFAA1EBC" modified="2012-04-18" version="2">NWMNELPQHLKDAPLMSLAIPGSHDSCAFNLNKNGPLAPDESKYFEPFKGVVYNWSITQELPIMDQLNIGIRYFDLRIAPKPDSEDLYFVHGLYGPAVQSVLENINQFLNYHPSEVVILDFNHLLEFKDEACHRKLIGMITSIFAGKLCPRQPTLANVTLTNLNNKSQQVIALYKYPVPNNLLWNQLYIKSPWLNKQNSTEVIKLLKENIKNKPSDVLYVSQGILTPDTMTIVGGLCSNLLNWEAGLNHKVMTWLDSKPDVNIIIIDDCNQEFADKVIAINK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>F6VS75</accession>
    <name>F6VS75_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
            <orientation evidence="1">Nucleoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NEMP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="F6VS75"/>
    <dbReference type="STRING" id="7719.ENSCINP00000000660"/>
    <dbReference type="Ensembl" id="ENSCINT00000000660.3">
        <property type="protein sequence ID" value="ENSCINP00000000660.3"/>
        <property type="gene ID" value="ENSCING00000000367.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000002174"/>
    <dbReference type="HOGENOM" id="CLU_1844406_0_0_1"/>
    <dbReference type="InParanoid" id="F6VS75"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005637">
        <property type="term" value="C:nuclear inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019358">
        <property type="entry name" value="NEMP_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13598">
        <property type="entry name" value="AT07567P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13598:SF1">
        <property type="entry name" value="AT07567P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10225">
        <property type="entry name" value="NEMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000000660.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="139" mass="16266" checksum="C5DDB88004173DCA" modified="2012-04-18" version="2">MFGLQFVKNLKRIKLLNWMLGSYGDPPPPKLLSEDEYYIQGKIETQKALEELKQYCRSPQCSPWKTLNRIDSPKRFASFIEGDSHILESEMTLYEEDMQLLMDEEQALIDNFISEDESEDQIKLNDETTEEDMGFAEDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>F6VTF7</accession>
    <name>F6VTF7_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Transcription factor protein</fullName>
        </submittedName>
    </protein>
    <gene>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
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                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
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                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
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                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
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                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
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                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
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    <dbReference type="InParanoid" id="F6VTQ9"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="62"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000024312.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="199" mass="23895" checksum="B02056C488ED6E38" modified="2012-04-18" version="2">MENTSKDSGFNTESPSSSLDSEEQFINWSEDESAPDSESEFNRQRRRDLLDVLVEIVLKQRRGNRHERERARRRRRHRRHLYERNSRSRPDTPEPLMKGKRRKCPIHDCGKRFINVTHHYTKYHPDVDKQLFCYDNLAKELGIGKYKNYVGFKCSFCGQMRMCFEAHCRNVHINEVDVKSPEFQTIKENCRISHKVRHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>F6VZ53</accession>
    <name>F6VZ53_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 7">Lysozyme g</fullName>
            <ecNumber evidence="3 7">3.2.1.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100176087</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>Hydrolysis of (1-&gt;4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.</text>
            <dbReference type="EC" id="3.2.1.17"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the glycosyl hydrolase 23 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.17" evidence="3 7"/>
    <dbReference type="AlphaFoldDB" id="F6VZ53"/>
    <dbReference type="Ensembl" id="ENSCINT00000015120.3">
        <property type="protein sequence ID" value="ENSCINP00000015120.3"/>
        <property type="gene ID" value="ENSCING00000007365.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000017614"/>
    <dbReference type="HOGENOM" id="CLU_089081_1_0_1"/>
    <dbReference type="InParanoid" id="F6VZ53"/>
    <dbReference type="OMA" id="CQGRTDC"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003796">
        <property type="term" value="F:lysozyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031640">
        <property type="term" value="P:killing of cells of another organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009253">
        <property type="term" value="P:peptidoglycan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01021">
        <property type="entry name" value="GEWL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.530.10:FF:000026">
        <property type="entry name" value="Lysozyme g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.530.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002152">
        <property type="entry name" value="Glyco_hydro_23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31698:SF11">
        <property type="entry name" value="LYSOZYME G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31698">
        <property type="entry name" value="LYSOZYME G FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001065">
        <property type="entry name" value="Lysozyme_g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00749">
        <property type="entry name" value="LYSOZYMEG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="Lysozyme-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0929">Antimicrobial</keyword>
    <keyword evidence="5" id="KW-0081">Bacteriolytic enzyme</keyword>
    <keyword evidence="7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR001065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000015120.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="186" mass="19885" checksum="DD2F60DC47A2041B" modified="2012-04-18" version="2">YTGCYGNIYNTDTTGCSSQTSSQDNLGYSGISASRQMAATDLSRMNQYKSNIENAGRQLCMDPSVIAGIISRETRAGATIGSDGYGADGHGYGLMQVDDRYHTIQGGPTSLDHILQGTGILISNIGQISSKFPSWDQNMDLKGGICAYNIGVGGVWSYDNMDVGTTGDDYSSDVVARAQYYKDNGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>F6W097</accession>
    <name>F6W097_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Sugar phosphate exchanger 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Solute carrier family 37 member 3</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Organophosphate:Pi antiporter (OPA) (TC 2.A.1.4) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="F6W097"/>
    <dbReference type="Ensembl" id="ENSCINT00000022524.2">
        <property type="protein sequence ID" value="ENSCINP00000022278.2"/>
        <property type="gene ID" value="ENSCING00000011719.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165554"/>
    <dbReference type="HOGENOM" id="CLU_001265_31_6_1"/>
    <dbReference type="InParanoid" id="F6W097"/>
    <dbReference type="OMA" id="YQWQVFL"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000028">
        <property type="entry name" value="Sugar phosphate exchanger 3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000849">
        <property type="entry name" value="Sugar_P_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43184">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY TRANSPORTER 16, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43184:SF12">
        <property type="entry name" value="SUGAR PHOSPHATE EXCHANGER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002808">
        <property type="entry name" value="Hexose_phosphate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="69"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="100"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="128"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="163"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="193"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="284"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="330"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="354"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="383"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="419"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="453"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="11">
        <location>
            <begin position="1"/>
            <end position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00041091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00042039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000022278.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="491" mass="54263" checksum="508ADABB6A13F219" modified="2012-04-18" version="2">TSHHVAVFILTFFCYVWFHATRKTFSNVKDDISKEWTPTPCNLTWPCPSPNKTWSSHNLFESYDDAKPFLGLLDTVFMFCYAVGLYFSGIIGDRFELRKVLAVGMWLSGILVFTFGTLTQWVHFYNKAFYIVIWGLNGLAQSCGWPSVVAVMGNWFGKSSRGLIFGLWSSCASVGNIIGTLLASQVIDYGYEQAFLVTSVPLFCTGFIVFFGLVSSPTEIGLSDPNKVEPEIKPDETVCFQLASIREIISDDEPLVVGSNPSVNEEDGDKNENETKPIGFFRAILLPGVIPYSLAYGCLKLVNYAFFFWLPYYLTNNFKWDDSKADDVSTWYDVGGIIGGIVFGVITDRFENRAPVLVGMLLVAPFTVWGYERSPNNVAINSVLMSLIGIFVGGASNIISSAVTAELGKQPAVGQNVDALATVTGILDGTSSIFAAVGQYLVAVIQTNLGWDWVFYSFIIMIILALICLIPMIANDVKTLWLKTRLTSSNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>F6W7G8</accession>
    <name>F6W7G8_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="F6W7G8"/>
    <dbReference type="Ensembl" id="ENSCINT00000002816.3">
        <property type="protein sequence ID" value="ENSCINP00000002816.3"/>
        <property type="gene ID" value="ENSCING00000020620.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163850"/>
    <dbReference type="HOGENOM" id="CLU_1859672_0_0_1"/>
    <dbReference type="InParanoid" id="F6W7G8"/>
    <dbReference type="OMA" id="WTEMEIV"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050382">
        <property type="entry name" value="MFS_Na/Anion_cotransporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11662:SF399">
        <property type="entry name" value="FI19708P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11662">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="23"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="48"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="78"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="116"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000002816.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="138" mass="15130" checksum="35B3242B609226C8" modified="2012-04-18" version="2">MSGFLSDFILLKTQIRKTFLRKVFSSIGLGGTALSFLILPSFGCNHTLAIVLICTSYMFVGCNYSGYRVALADMAPSYLGIMYAISNTFSCLLVLFATQIVGIMLGDGSLPYWKTVFYVTAAISAFGLFVYLWFGTSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>F6WZT7</accession>
    <accession>F6X021</accession>
    <name>F6WZT7_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Prp31 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="F6WZT7"/>
    <dbReference type="STRING" id="7719.ENSCINP00000005746"/>
    <dbReference type="Ensembl" id="ENSCINT00000005746.3">
        <property type="protein sequence ID" value="ENSCINP00000005746.3"/>
        <property type="gene ID" value="ENSCING00000022625.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSCINT00000005756.3">
        <property type="protein sequence ID" value="ENSCINP00000005756.3"/>
        <property type="gene ID" value="ENSCING00000016268.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00550000075069"/>
    <dbReference type="HOGENOM" id="CLU_2364890_0_0_1"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000244">
        <property type="term" value="P:spliceosomal tri-snRNP complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027105">
        <property type="entry name" value="Prp31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019175">
        <property type="entry name" value="Prp31_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13904">
        <property type="entry name" value="PRE-MRNA SPLICING FACTOR PRP31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13904:SF0">
        <property type="entry name" value="U4_U6 SMALL NUCLEAR RIBONUCLEOPROTEIN PRP31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09785">
        <property type="entry name" value="Prp31_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <feature type="domain" description="Prp31 C-terminal" evidence="4">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF09785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000005756.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="96" mass="10343" checksum="7390BDC6A695E7FC" modified="2012-04-18" version="2">QVDNKTQVRISKTLQTKIQRANRAGSTTSVTWGGKSTVRDRGSGIASSVAFTPLKGIEIVNPNAAEKKQEEEDGCSTYFSNTASFVKLKPRNVPSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>F6X4I5</accession>
    <name>F6X4I5_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
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        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="entry name" value="GH01279p"/>
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        <property type="entry name" value="Ribonuclease Inhibitor"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="464" mass="51665" checksum="B468D8C813E29840" modified="2012-04-18" version="2">MRNNVIGALPASVAKLKKLSSLNVSDNRLKALPKTIHKASGLETCDVSNNKIKKLPKNIGQSNMTSLDVSQNSIKILPKSIYRLPRSLDVSGNQIEVLPPIVITKKSKGNISQINLSDNKLSKLPEDIDKLQRLTHINVSGNHLQSLPTAIGSLKYLHHLDASSNNIKVFPQSVYSLGFSLTSLNLTNNSLESIPDGIGSLKRLRVFMLSFNKLQTLPNDMNGLVSLQQLHVSNNNLTNLSPLIGLLHLETLDASNNKITSLPTEMHTLRWLFILNLANNQLTSLPPSIGHIKALKQLYLRNNRLESLPSTLGEQQLLQVVDASDNELTNLPDDLRKWRTLEQLILARNQLSQIPGNIGRLRLLEILDVSNNQVARIPEQIGILTHLQELHVSNNCLENFAQNLTKLRQILNLQIINLSRNDLKLLPIDIWRMKSLTSLDLTDNLIESIPESIAKLPALKVCDV</sequence>
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    <accession>F6XES4</accession>
    <accession>A0A1W5B9G5</accession>
    <name>F6XES4_CIOIN</name>
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        <submittedName>
            <fullName evidence="3">Uncharacterized LOC100180798</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100180798</name>
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    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01002880">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_026691821.1">
        <property type="nucleotide sequence ID" value="XM_026836020.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6XES4"/>
    <dbReference type="Ensembl" id="ENSCINT00000011487.3">
        <property type="protein sequence ID" value="ENSCINP00000011487.3"/>
        <property type="gene ID" value="ENSCING00000005545.3"/>
    </dbReference>
    <dbReference type="GeneID" id="100180798"/>
    <dbReference type="KEGG" id="cin:100180798"/>
    <dbReference type="GeneTree" id="ENSGT00390000002041"/>
    <dbReference type="HOGENOM" id="CLU_1776802_0_0_1"/>
    <dbReference type="InParanoid" id="F6XES4"/>
    <dbReference type="OrthoDB" id="10063348at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005126">
        <property type="term" value="F:cytokine receptor binding"/>
        <property type="evidence" value="ECO:0000318"/>
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        <property type="term" value="P:negative regulation of receptor signaling pathway via JAK-STAT"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <location>
            <begin position="23"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000011487.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="146" mass="16814" checksum="CD4F99AE389B76C0" modified="2012-04-18" version="2">MRDNGSNICTGKVRCFCAERSPFYWRKLNAKSATKMLINRPIGSYLLRPSEHPLYDYSITQKIPVMGVTHIRVGRDSTGTYFLDNDDSFSEAVIKGFTCVVSMLDCWSRVYRERGTELSTIACTSNVTLERSRLLRQRRLEQCLFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
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            <fullName evidence="9">Small fragment nuclease</fullName>
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    <organism evidence="11 12">
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        <name type="common">Transparent sea squirt</name>
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        <lineage>
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            <taxon>Chordata</taxon>
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            <taxon>Phlebobranchia</taxon>
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        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">wild type</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">3'-to-5'exoribonuclease that preferentially degrades DNA and RNA oligonucleotides composed of only two nucleotides. Binds and degrades longer oligonucleotides with a lower affinity. Plays dual roles in mitochondria, scavenging nanoRNAs (small RNA oligonucleotides of &lt;5 nucleotides) that are produced by the degradosome and clearing short RNAs that are generated by RNA processing. Essential for correct initiation of mitochondrial transcription, degrading mitochondrial RNA dinucleotides to prevent RNA-primed transcription at non-canonical sites in the mitochondrial genome. Essential for embryonic development.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer. Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the oligoribonuclease family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001782">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6XG50"/>
    <dbReference type="FunCoup" id="F6XG50">
        <property type="interactions" value="648"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000019978"/>
    <dbReference type="Ensembl" id="ENSCINT00000019978.3">
        <property type="protein sequence ID" value="ENSCINP00000019978.3"/>
        <property type="gene ID" value="ENSCING00000009858.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000009255"/>
    <dbReference type="HOGENOM" id="CLU_064761_2_0_1"/>
    <dbReference type="InParanoid" id="F6XG50"/>
    <dbReference type="OMA" id="AFFHYRN"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06135">
        <property type="entry name" value="Orn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000003">
        <property type="entry name" value="Oligoribonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022894">
        <property type="entry name" value="Oligoribonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013520">
        <property type="entry name" value="Ribonucl_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003765">
        <property type="entry name" value="PRK05359.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11046">
        <property type="entry name" value="OLIGORIBONUCLEASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11046:SF0">
        <property type="entry name" value="OLIGORIBONUCLEASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00929">
        <property type="entry name" value="RNase_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00479">
        <property type="entry name" value="EXOIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exonuclease" evidence="10">
        <location>
            <begin position="33"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00046824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00057450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00068437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00079509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00079573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000019978.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="205" mass="23615" checksum="DCD75E03D0CD7DF1" modified="2011-07-27" version="1">MIQCCRTLLTLSSSNLVRIVCNFAMAGDDARNRMVWVDLEMTGLDVNKDHILEMACLVTDGNLNVIAQEEDLVIHQSDEVLNNMNEWCIEQHGRSGLTESVRKSMITLTQAEDIMLKFIQKHAPYKVCPLAGNTIHADKRFLDHYMPRFMDHLHYRIVDVSSIKELSKRWYPNVLPPPKGSSHRALEDILESIAELKFYRKTLFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>F6XIT4</accession>
    <name>F6XIT4_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FUZ/MON1/HPS1 first Longin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">wild type</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01001608">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6XIT4"/>
    <dbReference type="STRING" id="7719.ENSCINP00000021696"/>
    <dbReference type="Ensembl" id="ENSCINT00000021942.2">
        <property type="protein sequence ID" value="ENSCINP00000021696.2"/>
        <property type="gene ID" value="ENSCING00000011325.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015298"/>
    <dbReference type="HOGENOM" id="CLU_070340_0_0_1"/>
    <dbReference type="InParanoid" id="F6XIT4"/>
    <dbReference type="OMA" id="TKERAWH"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043972">
        <property type="entry name" value="FUZ/MON1/HPS1_longin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026053">
        <property type="entry name" value="HPS1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12761:SF1">
        <property type="entry name" value="BLOC-3 COMPLEX MEMBER HPS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12761">
        <property type="entry name" value="HERMANSKY-PUDLAK SYNDROME PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19036">
        <property type="entry name" value="Fuz_longin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FUZ/MON1/HPS1 first Longin" evidence="2">
        <location>
            <begin position="6"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="237"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="241"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000021696.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="273" mass="30858" checksum="B1619B2BCD4BB379" modified="2012-04-18" version="2">MKAYVIAEDTHIVYYWCDEAFVTHLKSLRNIQDHDQQSESSFDSYIQMYFLPVFLSHQMLKSQLNEACSSVSSDDQDTVVVFQDFQEFVYIAVETSCSGGKEKLSRELSFLRNSISFLFGACGHSELKTTQVTTKERAWHSVTTILQQYTSLCELENSFLLEGVERIRVNEDVNKVCNTVIQKSMKSFESLSNLHNVQYGLVFVGAKILTLHASSRARYNLSTQNILQIITLSSLGSGQTDSEHEVRSDSELESSEIPSDIGLDLPFIPSTDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>F6XLJ0</accession>
    <name>F6XLJ0_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">wild type</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
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    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="ENSCING00000024813.1"/>
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    <dbReference type="HOGENOM" id="CLU_616160_0_0_1"/>
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        <property type="entry name" value="Plasminogen Kringle 4"/>
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            <dbReference type="SAM" id="SignalP"/>
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</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F6XRG8</accession>
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            <fullName evidence="2">Uncharacterized protein</fullName>
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        <name type="scientific">Ciona intestinalis</name>
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            <taxon>Phlebobranchia</taxon>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="protein sequence ID" value="ENSCINP00000005163.3"/>
        <property type="gene ID" value="ENSCING00000020454.1"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="1">
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    <sequence length="387" mass="43586" checksum="3837669825D20E21" modified="2012-04-18" version="2">MRIIERTGLVNRDEVPEDPNARVTYNAAHSQRNTDATKWFGQAKPKDKVWLKPKGKVDDSAETERRKVIKAMTKKHNPNYVPKWKPPVEKKWEDTLDENKKESLKKYEERRRQRIESSSAHSTSSSSDVTSASEVSISIHSASLSELCPNSEVVVESNVHSKDAINLWESKLSDKFAKQETENKRLSVGKWKRAQRQAQEKRERTESMSSVLSNGSVTGDLPTSENVIRESLGVSNIMSKWRNIEHQSQENKSLTNFRKTAAAPRRTNSFTVTPGSGYRSFASPTKTAAPASPKVPTSPEGPPVNSARYSQPIPGESFIDREIRESAEREEQWKHEKLEPEIDRLSMTSSNESTTYMKLPSGAIYSPTLSPAFAMTYVSVTNDKSVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>F6XUA1</accession>
    <accession>A0A1W2WFJ2</accession>
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            <fullName evidence="2">Negative elongation factor B</fullName>
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    </protein>
    <gene>
        <name evidence="2" type="primary">LOC100186426</name>
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    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_002130034.1">
        <property type="nucleotide sequence ID" value="XM_002129998.5"/>
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    <dbReference type="STRING" id="7719.ENSCINP00000008498"/>
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        <property type="protein sequence ID" value="ENSCINP00000008498.3"/>
        <property type="gene ID" value="ENSCING00000004122.3"/>
    </dbReference>
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    <dbReference type="KEGG" id="cin:100186426"/>
    <dbReference type="GeneTree" id="ENSGT00390000012665"/>
    <dbReference type="HOGENOM" id="CLU_037919_0_0_1"/>
    <dbReference type="InParanoid" id="F6XUA1"/>
    <dbReference type="OrthoDB" id="5548359at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
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    <dbReference type="GO" id="GO:0032021">
        <property type="term" value="C:NELF complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="P:negative regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010405">
        <property type="entry name" value="COBRA1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13503:SF3">
        <property type="entry name" value="NEGATIVE ELONGATION FACTOR B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13503">
        <property type="entry name" value="NEGATIVE ELONGATION FACTOR COMPLEX MEMBER B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06209">
        <property type="entry name" value="COBRA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="401"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="418"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000008498.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="605" mass="68887" checksum="E206EB5EC8F51D6C" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>F6Y4I3</accession>
    <name>F6Y4I3_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CUB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">wild type</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01001485">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6Y4I3"/>
    <dbReference type="STRING" id="7719.ENSCINP00000009133"/>
    <dbReference type="Ensembl" id="ENSCINT00000009133.3">
        <property type="protein sequence ID" value="ENSCINP00000009133.3"/>
        <property type="gene ID" value="ENSCING00000004421.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166030"/>
    <dbReference type="HOGENOM" id="CLU_015228_7_1_1"/>
    <dbReference type="InParanoid" id="F6Y4I3"/>
    <dbReference type="OMA" id="SVAPFNY"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000013">
        <property type="entry name" value="Membrane frizzled-related protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000005">
        <property type="entry name" value="Procollagen C-endopeptidase enhancer 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24251:SF30">
        <property type="entry name" value="MEMBRANE FRIZZLED-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24251">
        <property type="entry name" value="OVOCHYMASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
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    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CUB" evidence="4">
        <location>
            <begin position="30"/>
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        </location>
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        <location>
            <begin position="155"/>
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        </location>
    </feature>
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        <location>
            <begin position="272"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000009133.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="386" mass="41740" checksum="87485E79DC027427" modified="2012-04-18" version="2">MYMHYHTDGSVHSTGFQFRFSVSASVTPSCDQLSIGGAPGSGVITSPNYPLNYPHHSDCRYRICSDSNSTITLEFSAFNLESHNDCKYDFVDVRSGSDGDWTSSALKSCGTDLPATLTFSQQCVYIQFFSDSSVSKTGFSLRWTIAGLEPGPPGCTQLNLGGAPGSDGLIKSANFPSNYPINLDCRYQVYSDCSSNNIAFTFLAFDVESHTLCNYDYVELLNGCSSTAASQGKFCGTTIPAPTTNLATTCRAMRFKTDGSVPKTGFNMRYTVTGSCYPSSGVITSPGFPNTHHNNAENNYVISPPGATAIRLQFTTFELEGSAPSCLYDSVTIRDNSKTGTILDTFCGNMTTPFTKTYAVSKLVLMFKSDSTVINPGFSCYFSKVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>F6Y6F9</accession>
    <name>F6Y6F9_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
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        <lineage>
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        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
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                <person name="Nomoto H."/>
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                <person name="Oishi K."/>
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                <person name="Sasaki A."/>
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                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">wild type</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
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                <consortium name="Ensembl"/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="De Tomaso A."/>
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                <person name="Goodstein D.M."/>
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                <person name="Terry A."/>
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                <person name="Awazu S."/>
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                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
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                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
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                <person name="Doggett N."/>
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                <person name="Richardson P."/>
                <person name="Lucas S."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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    <sequence length="213" mass="23550" checksum="FB238CE0703B78E4" modified="2012-04-18" version="2">MTLIDRFCSFLLCIALLNPVSARYLSICEGGRTTVTCLPGYVIDIREAFYGRDNQHTCPSGTIHTTTCRASTSETLIRGKCQGRNSCSLLASNSVFGDSCRGTFKYIYLEYQCKENVNSGVRTATICENSQSTISCEQGSTINVLDSFYGRIDRTTCPSGNTYTTSCKAVHSPIYIRGLCQSRKTCTIRATNSVFGDPCYGTYKYVQIKYVCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
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    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
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            <taxon>Ciona</taxon>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01002340">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6YGC9"/>
    <dbReference type="STRING" id="7719.ENSCINP00000008746"/>
    <dbReference type="Ensembl" id="ENSCINT00000008746.3">
        <property type="protein sequence ID" value="ENSCINP00000008746.3"/>
        <property type="gene ID" value="ENSCING00000023190.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000168947"/>
    <dbReference type="HOGENOM" id="CLU_654778_0_0_1"/>
    <dbReference type="InParanoid" id="F6YGC9"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="95"/>
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        </location>
    </feature>
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        </location>
    </feature>
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        </location>
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        </location>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000008746.3"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
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                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
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                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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            </authorList>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">wild type</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01002689">
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="F6YN02"/>
    <dbReference type="Ensembl" id="ENSCINT00000004591.3">
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        <property type="gene ID" value="ENSCING00000013215.2"/>
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        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006413">
        <property type="term" value="P:translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036788">
        <property type="entry name" value="T_IF-3_C_sf"/>
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        <property type="match status" value="1"/>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000004591.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="118" mass="13987" checksum="5FFA8D025372F43A" modified="2011-07-27" version="1">MNSMVWNAVRQTRNILNQNNQSLSTGQHFFAKKENVLKFKHSVERDHLLYKAKQISDWLSKERNVKVMFYGYKDQQKLEDVSNFLKDKVSENCNFEMTETKQPKGLNLYFTPSMSKDT</sequence>
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<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>F6YRG2</accession>
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            <fullName evidence="6">Caveolin</fullName>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
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                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
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                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Putnam N."/>
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                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
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                <person name="Rigoutsos I."/>
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                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Cell membrane</location>
            <topology evidence="1 6">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Golgi apparatus membrane</location>
            <topology evidence="6">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Membrane</location>
            <location evidence="6">Caveola</location>
            <topology evidence="6">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the caveolin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="F6YRG2"/>
    <dbReference type="STRING" id="7719.ENSCINP00000002792"/>
    <dbReference type="Ensembl" id="ENSCINT00000002792.3">
        <property type="protein sequence ID" value="ENSCINP00000002792.3"/>
        <property type="gene ID" value="ENSCING00000001428.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183006"/>
    <dbReference type="HOGENOM" id="CLU_102582_0_0_1"/>
    <dbReference type="InParanoid" id="F6YRG2"/>
    <dbReference type="OMA" id="TRIFMDD"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005901">
        <property type="term" value="C:caveola"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070836">
        <property type="term" value="P:caveola assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001612">
        <property type="entry name" value="Caveolin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10844">
        <property type="entry name" value="CAVEOLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10844:SF32">
        <property type="entry name" value="CAVEOLIN-3-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01146">
        <property type="entry name" value="Caveolin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5 6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="144"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="7"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="39"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000002792.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="211" mass="24146" checksum="6DBEF5C5A85EEB17" modified="2011-07-27" version="1">MGGEKTGSQDDVARLPVDDVTCDVTSGEEFVVVDETEPKEEGDSSKHPSRDASPSNQEVEEPKKKKKKKRVRGLDLPVQVFKSRSERDPEFINQDLRVNFDDVIAEPEGYYTPKYSWHLSKEVYGFSKGCCYSVASFFCSVPAAFLWGIWFAFIACLNIWLLVPFRRCHNIKMSLWKGFWGVTVSSIFDPIFQSIGMVFNNMRVQTDKYEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>F6YUW7</accession>
    <name>F6YUW7_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ZHX family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="F6YUW7"/>
    <dbReference type="STRING" id="7719.ENSCINP00000026105"/>
    <dbReference type="Ensembl" id="ENSCINT00000026351.1">
        <property type="protein sequence ID" value="ENSCINP00000026105.1"/>
        <property type="gene ID" value="ENSCING00000014429.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182893"/>
    <dbReference type="HOGENOM" id="CLU_414438_0_0_1"/>
    <dbReference type="InParanoid" id="F6YUW7"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000907">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000062">
        <property type="entry name" value="zinc fingers and homeoboxes protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008422">
        <property type="entry name" value="KN_HD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15467:SF9">
        <property type="entry name" value="HOMEOBOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15467">
        <property type="entry name" value="ZINC-FINGERS AND HOMEOBOXES RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05920">
        <property type="entry name" value="Homeobox_KN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0221">Differentiation</keyword>
    <keyword evidence="11 15" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="12 15" id="KW-0371">Homeobox</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14 15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="13" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Homeobox" evidence="16">
        <location>
            <begin position="373"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="16">
        <location>
            <begin position="555"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="15">
        <location>
            <begin position="375"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="15">
        <location>
            <begin position="557"/>
            <end position="616"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000026105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="662" mass="73885" checksum="4A11EDC290813807" modified="2011-07-27" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>F6Z0V4</accession>
    <name>F6Z0V4_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">COP9 signalosome complex subunit 7a-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC113474404</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">wild type</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, JUN, I-kappa-B-alpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CSN7/EIF3M family. CSN7 subfamily.</text>
    </comment>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_026835335.1"/>
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    <dbReference type="STRING" id="7719.ENSCINP00000010734"/>
    <dbReference type="Ensembl" id="ENSCINT00000010734.3">
        <property type="protein sequence ID" value="ENSCINP00000010734.3"/>
        <property type="gene ID" value="ENSCING00000021617.1"/>
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    <dbReference type="KEGG" id="cin:113474404"/>
    <dbReference type="GeneTree" id="ENSGT00940000169585"/>
    <dbReference type="HOGENOM" id="CLU_054426_2_0_1"/>
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        <property type="component" value="Chromosome 1"/>
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        <property type="term" value="C:COP9 signalosome"/>
        <property type="evidence" value="ECO:0000318"/>
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    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0010387">
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="COPS7/eIF3m"/>
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        <property type="entry name" value="PCI_dom"/>
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    <dbReference type="PANTHER" id="PTHR15350:SF5">
        <property type="entry name" value="COP9 SIGNALOSOME COMPLEX SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15350">
        <property type="entry name" value="COP9 SIGNALOSOME COMPLEX SUBUNIT 7/DENDRITIC CELL PROTEIN GA17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22061">
        <property type="entry name" value="CSN7_HB_subdom"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF18392">
        <property type="entry name" value="CSN7a_helixI"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PCI"/>
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        <property type="entry name" value="PCI"/>
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            <begin position="1"/>
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            <begin position="231"/>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    </evidence>
    <sequence length="283" mass="31584" checksum="2C6804355C06278E" modified="2011-07-27" version="1">MDNIVTQKTNKSECLNKNVHLDPFLILAKSAKGAALKSLIEQVLEAPGVYVFGELLELQCIQQLVNSEHNKHLKLLELFTYGTFQDYVNQAYGLPPLTPLMKKKLHQLTVVALSAKSKYVSYESLMKELHLNNIRDLEDLLISAIYANIIQGRLDQQNSRLEVEWAMGRDVRTEDMDSIINTLNNWCSSCETILGSIETQTASANAFLSEDAIRRAKIDTEVENIMSTIKKTSSHEMDLATSDTRLSSSHPAPSSGIEVKPKKSKVKGLRGSGMKLWSSKNTS</sequence>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    <dbReference type="InParanoid" id="F6Z6B9"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000028516.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    </evidence>
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            <fullName evidence="7">Integrator complex subunit 4</fullName>
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    <reference evidence="6" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="5" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome 12"/>
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        <location>
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            <end position="206"/>
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            <end position="342"/>
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        <source>
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    </evidence>
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        <source>
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            <dbReference type="Pfam" id="PF24493"/>
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        <source>
            <dbReference type="Pfam" id="PF25458"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    </evidence>
    <sequence length="346" mass="38573" checksum="484D221AEB727B43" modified="2012-04-18" version="2">MDRVKNLQVYEPSEQRKLLDCTIRDLRHAADVAPSLASTAQCSADYLQAQLLLSKAVDTRSWTGSSHICAQHTAFAKPAANQIIQLTKELEHLYLGLSTNEIALVLQLRLKAQALLFVLEMQEATLSRKNKSQSSGEKPLKPSKLCEEFFHRVQELKSILEKSDIAPDSFSRTLFNELLKLSASRKPSLVVKLLQPLLSHYSASEVDLNNKVQKSRVIIHEPQPNPDSPVVISAGLVAALNVDAVAENLTNPLHEIRIQVIYPDSTSQLFNLSPQDVKQSTPFKHKISTKIYLSHEQWSEACVVKLLFVKIVRAPLDVKHNTTTSETLPLSKAVEVTFHPKPASRA</sequence>
</entry>
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            <fullName evidence="1">Uncharacterized protein</fullName>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">wild type</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="entry name" value="TSP1_rpt_sf"/>
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        <property type="entry name" value="TSP-1 type 1 repeat"/>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="215" mass="23569" checksum="69C2FCAE039546ED" modified="2012-04-18" version="2">MSWGICFKSCGSGIRYRSRHCKKGHYFSYGCPGRHHELGYCNTFSCTSSFANDPPSPMFSFDSKSVANLCSGANSDVVFIFGDFNGDRNVDVMCGNQDGDFEVGLVTSNGDINTASWRGRLDGCVVQSGQIAVGDFNGDAMSDIVCVDRVKKKTTVRLSNNGQFPTGEGYSGSFCTDDSDWLIPLDIDGDSKWDFMCSHNNRDHEILMNSFTNPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
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    <name>F6ZJN8_CIOIN</name>
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            <fullName evidence="6">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Ciona intestinalis</name>
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        <name type="synonym">Ascidia intestinalis</name>
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    <reference evidence="8" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
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                <person name="De Tomaso A."/>
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                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
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                <person name="Hastings K.E."/>
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                <person name="Meinertzhagen I.A."/>
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                <person name="Putnam N."/>
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                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
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                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
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                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
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                <person name="Miura S."/>
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                <person name="Ohta F."/>
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                <person name="Shin-i T."/>
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                <person name="Stainier D."/>
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                <person name="Tassy O."/>
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                <person name="Tokuoka M."/>
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                <person name="Yoshizaki F."/>
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                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <comment type="subcellular location">
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            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
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    <dbReference type="STRING" id="7719.ENSCINP00000015598"/>
    <dbReference type="Ensembl" id="ENSCINT00000015598.3">
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        <property type="gene ID" value="ENSCING00000007605.3"/>
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    <dbReference type="InParanoid" id="F6ZJN8"/>
    <dbReference type="OMA" id="VHENTRR"/>
    <dbReference type="Proteomes" id="UP000008144">
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        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0031625">
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        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="entry name" value="Ubl_ubiquitin"/>
        <property type="match status" value="5"/>
    </dbReference>
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        <property type="entry name" value="Ubiquitin-60S ribosomal L40 fusion"/>
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    </dbReference>
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        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
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        <property type="entry name" value="Ubiquitin-like_domsf"/>
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        <property type="entry name" value="Ubiquitin_CS"/>
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        <property type="entry name" value="Ubiquitin_dom"/>
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    <dbReference type="InterPro" id="IPR050158">
        <property type="entry name" value="Ubiquitin_ubiquitin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10666">
        <property type="entry name" value="UBIQUITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
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    </dbReference>
    <dbReference type="PRINTS" id="PR00348">
        <property type="entry name" value="UBIQUITIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="5"/>
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        <property type="entry name" value="UBIQUITIN_2"/>
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    <proteinExistence type="predicted"/>
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    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
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        <location>
            <begin position="77"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="6">
        <location>
            <begin position="153"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="6">
        <location>
            <begin position="229"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="6">
        <location>
            <begin position="305"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000015598.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="381" mass="42613" checksum="406B0AAE59301BC7" modified="2012-04-18" version="2">MQIFVKTLTGKTITLEVEASDSIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEASDSIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEASDSIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEASDSIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEASDSIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>F6ZM65</accession>
    <name>F6ZM65_CIOIN</name>
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        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">wild type</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the WD repeat POC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002133">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="F6ZM65"/>
    <dbReference type="STRING" id="7719.ENSCINP00000018966"/>
    <dbReference type="Ensembl" id="ENSCINT00000018966.3">
        <property type="protein sequence ID" value="ENSCINP00000018966.3"/>
        <property type="gene ID" value="ENSCING00000009330.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157494"/>
    <dbReference type="HOGENOM" id="CLU_000288_57_17_1"/>
    <dbReference type="InParanoid" id="F6ZM65"/>
    <dbReference type="OMA" id="LMIWHFK"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
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        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0048871">
        <property type="term" value="P:multicellular organismal-level homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000235">
        <property type="entry name" value="POC1 centriolar protein homolog B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
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        <property type="entry name" value="WDR55/POC1"/>
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        <property type="entry name" value="POC1 CENTRIOLAR PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44019">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
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        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
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        <property type="match status" value="7"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
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    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="14"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="56"/>
            <end position="87"/>
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    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="98"/>
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    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="140"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="182"/>
            <end position="223"/>
        </location>
    </feature>
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        <location>
            <begin position="224"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="266"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="307"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="375"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="440"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="336"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="376"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000018966.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="483" mass="54401" checksum="760182B2AAA4831A" modified="2012-04-18" version="2">MALAMNDPTLERQFKGHRDTITSCDFNPNMKQLATGSMDSCLMVWNFKPNMRSYRFVGHKDAILSVQFSPSGHLVASASRDKTVRLWIPSVKGESTVFKAHTATVRSVNFSSDGLHLLTAADDKTIKVWSVHRQKFQFSLTQHMNWVRCARFSPDNRLIVSGSDDKTVKLWDRNSKECVHTYFQHGGFVNHVEFHPSGTCIAAAGTDSTVKVWDIRTNKLLQHYQVHSGPVNQLSFHPSGNFLITSSNDCTLKIMDLLEGRLFFTLHGHQEPVTATTFSRNGEYFASGGADEQVIVWKTNFDKSDLTERPLTQTTHEAKPTGLREPTFKQGNNNPPTQPLTPPRRQPFHSTGEPKNMMATKGSDIMDIGPMLSHSTMEHPESEAGRARKQLADRSMKTPTKTNVEDVSSQIYKMTFSRFGTSITAPLEEKSIPPKLAATLEQIVGQLDMLTQTMTILEQRMSMTEDKVKECLDNQHQMTRHTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F6ZNE0</accession>
    <name>F6ZNE0_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
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    <reference evidence="8" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Choline transporter.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
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            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="ENSCINP00000014082.3"/>
        <property type="gene ID" value="ENSCING00000006870.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166330"/>
    <dbReference type="HOGENOM" id="CLU_017181_2_0_1"/>
    <dbReference type="InParanoid" id="F6ZXD5"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:transmembrane transporter activity"/>
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        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="InterPro" id="IPR007603">
        <property type="entry name" value="Choline_transptr-like"/>
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    <dbReference type="PANTHER" id="PTHR12385">
        <property type="entry name" value="CHOLINE TRANSPORTER-LIKE (SLC FAMILY 44)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CHOLINE TRANSPORTER-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF04515">
        <property type="entry name" value="Choline_transpo"/>
        <property type="match status" value="1"/>
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    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
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        <location>
            <begin position="33"/>
            <end position="55"/>
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    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="209"/>
            <end position="229"/>
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    <feature type="transmembrane region" description="Helical" evidence="6">
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            <begin position="236"/>
            <end position="259"/>
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    <feature type="transmembrane region" description="Helical" evidence="6">
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            <end position="304"/>
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    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="334"/>
            <end position="356"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="382"/>
            <end position="409"/>
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    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="533"/>
            <end position="556"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="562"/>
            <end position="584"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="632"/>
            <end position="654"/>
        </location>
    </feature>
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        <location>
            <begin position="632"/>
            <end position="648"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007168"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU368066"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="Ensembl" id="ENSCINP00000014082.3"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="654" mass="72602" checksum="684DB00BEAF34773" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>F7A102</accession>
    <name>F7A102_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">wild type</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
            <location evidence="2">Centriolar satellite</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ADIP family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01002198">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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    <dbReference type="Ensembl" id="ENSCINT00000029323.2">
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    </dbReference>
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        <property type="term" value="C:anchoring junction"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:intraciliary transport involved in cilium assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="entry name" value="Adhesion_Centrosome_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021622">
        <property type="entry name" value="Afadin/alpha-actinin-bd"/>
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        <property type="entry name" value="AFADIN- AND ALPHA-ACTININ-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46507:SF4">
        <property type="entry name" value="SSX FAMILY MEMBER 2 INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ADIP"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
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        <location>
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        <location>
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        <location>
            <begin position="272"/>
            <end position="281"/>
        </location>
    </feature>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004607"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00009291"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000029077.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="452" mass="53009" checksum="8254FE9E3FC3412C" modified="2012-04-18" version="2">MESSEAGSVSSRSRSSSNQSSSDMFSKKHLTFCSPDTLLQRAEFLNQEISVLGYPAILVDDALDPVAMINVLHDLVNSYRQLLSCNSEISTELKSSECESARLEHLLLQAKDDVANIDRSLKSKCSEVSYVNEKFESFRKKLRIEQEKCRRLSLDLINRDAQYKHEKKKKAQEVERMQTKLQQLLQDKRHEKVLGIDMLNLMERPEGRRGKWTTEKSKKNNELEMYHMIINNYEDANKKLLEENSEIRKYLKQMQEELITALNEKSAANIADGNQGTNESGSKPDLVNGGVKISEPIRDNLFEMPYEMVKDDIERNLTLRWKAVQKEIQNKNLQTTVPDMNYIIVIQRINEHLYEMMRKMEECKKIIEEQQRLIDYLTTSQASTSSQHFSDSYFLEEKSELERNKGMFYQQRISFEKERQVYTDALIQLGIDRQKFEEQRSRWLQKHFLQLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>F7AAD5</accession>
    <name>F7AAD5_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
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            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
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                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">wild type</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    <sequence length="163" mass="20003" checksum="E5C00AB3C05A2480" modified="2011-07-27" version="1">ERSRERELDRDERARKSQRDIYHRAVSPYKHHPEDPPYPRREPPYPRSRDPRSDEPDWVEEFRRRRANDDDHVTRRAWVNDPRWKDKRREFDINEYERDVRKATSYNSDFEQPQRKQMFPSKLMAKGNPPPGRKHPGGNPRGNNDWMRDLKENNKKAAKNRFR</sequence>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
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                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
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                <person name="Harafuji N."/>
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                <person name="Ho I."/>
                <person name="Hotta K."/>
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                <person name="Kawashima T."/>
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                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
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                <person name="Branno M."/>
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                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
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                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
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                <person name="Inaba K."/>
                <person name="Kano S."/>
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                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
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                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
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                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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    <reference evidence="6" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
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                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
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                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
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                <person name="Miura S."/>
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                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
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                <person name="Shoguchi E."/>
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                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
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                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
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                <person name="Richardson P."/>
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                <person name="Kohara Y."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">wild type</strain>
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    <reference evidence="5" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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            <location evidence="1">Secreted</location>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="F7AG40"/>
    <dbReference type="Ensembl" id="ENSCINT00000026268.2">
        <property type="protein sequence ID" value="ENSCINP00000026022.2"/>
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    <dbReference type="GeneTree" id="ENSGT00940000173314"/>
    <dbReference type="HOGENOM" id="CLU_001074_8_2_1"/>
    <dbReference type="InParanoid" id="F7AG40"/>
    <dbReference type="OMA" id="MPIWSVH"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 14"/>
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    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="entry name" value="C1q_dom"/>
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        <property type="entry name" value="Cerebellin_Synaptic_Org"/>
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        <property type="entry name" value="Tumour_necrosis_fac-like_dom"/>
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        <property type="entry name" value="C1Q DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR22923">
        <property type="entry name" value="CEREBELLIN-RELATED"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <location>
            <begin position="26"/>
            <end position="171"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="PROSITE" id="PS50871"/>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000026022.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    <accession>F7AJC9</accession>
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            <fullName evidence="1">Uncharacterized protein</fullName>
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        <name type="scientific">Ciona intestinalis</name>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">wild type</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01000531">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="F7AJC9"/>
    <dbReference type="Ensembl" id="ENSCINT00000029561.2">
        <property type="protein sequence ID" value="ENSCINP00000029315.2"/>
        <property type="gene ID" value="ENSCING00000017229.2"/>
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    <dbReference type="GeneTree" id="ENSGT00940000172764"/>
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    <dbReference type="InParanoid" id="F7AJC9"/>
    <dbReference type="OMA" id="SSWNMER"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 10"/>
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    <dbReference type="PANTHER" id="PTHR33667:SF7">
        <property type="entry name" value="RIKEN CDNA 1810020O05 GENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33667">
        <property type="entry name" value="SI:DKEY-57N24.6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000029315.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="285" mass="33354" checksum="F064C48D0F48D907" modified="2011-07-27" version="1">MLLQQDPDARYAYHPTYNSAMLVPINVDAYYKEQDEAARRAWKTEQGMLFPGMKSSRECNEHPLKPDDSRIEELRLKFRDNVLHRNILEPTLQRDRMSWDHRKEDVELYRRPVSYFGSSPPVTIHLAGQLLKEEQLKNKRMEEEEWRSRVVVDEDRMKFHRVLPGTEQTNKSNQLDRLTCLLKDEPKKLALKRPPVPEIPPLSVVQNPSVDRAARARGVKLPPAFDESREKNVGFMVGPYDDSSWNMERNKVPIKDPHHQYFAEKWGHDMKCKENSVSTLHAANS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F7AJX9</accession>
    <name>F7AJX9_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
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    </protein>
    <organism evidence="5 6">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
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            <taxon>Ciona</taxon>
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    <reference evidence="6" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IL-17 family.</text>
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    <dbReference type="AlphaFoldDB" id="F7AJX9"/>
    <dbReference type="Ensembl" id="ENSCINT00000005082.3">
        <property type="protein sequence ID" value="ENSCINP00000005082.3"/>
        <property type="gene ID" value="ENSCING00000015638.2"/>
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    <dbReference type="InParanoid" id="F7AJX9"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
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    <dbReference type="GO" id="GO:0005576">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Cystine-knot_cytokine"/>
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    <dbReference type="InterPro" id="IPR010345">
        <property type="entry name" value="IL-17_fam"/>
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    <dbReference type="Pfam" id="PF06083">
        <property type="entry name" value="IL17"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000005082.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="88" mass="9803" checksum="115E2DA2AD6FE124" modified="2012-04-18" version="2">MTNISRRSLSPWTYIRNVDRTRIPVVMLDAVCLCGGCYDISSPSLAEDINLYSTRVPGTIPVRRIRPGKPSVPDYVNLHTGCRCVVPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>F7ALW9</accession>
    <accession>A0A1W2W9Y0</accession>
    <name>F7ALW9_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcription factor Adf-1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC100184915</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_002124233.1">
        <property type="nucleotide sequence ID" value="XM_002124197.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F7ALW9"/>
    <dbReference type="FunCoup" id="F7ALW9">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSCINT00000023210.2">
        <property type="protein sequence ID" value="ENSCINP00000022964.2"/>
        <property type="gene ID" value="ENSCING00000012255.2"/>
    </dbReference>
    <dbReference type="GeneID" id="100184915"/>
    <dbReference type="KEGG" id="cin:100184915"/>
    <dbReference type="GeneTree" id="ENSGT00940000167971"/>
    <dbReference type="HOGENOM" id="CLU_080630_2_0_1"/>
    <dbReference type="InParanoid" id="F7ALW9"/>
    <dbReference type="OMA" id="VECEINT"/>
    <dbReference type="OrthoDB" id="6147983at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006578">
        <property type="entry name" value="MADF-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039353">
        <property type="entry name" value="TF_Adf1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12243">
        <property type="entry name" value="MADF DOMAIN TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12243:SF69">
        <property type="entry name" value="SI:CH73-59F11.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10545">
        <property type="entry name" value="MADF_DNA_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00595">
        <property type="entry name" value="MADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51029">
        <property type="entry name" value="MADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MADF" evidence="2">
        <location>
            <begin position="20"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="125"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000022964.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="266" mass="30723" checksum="276CE9268568A330" modified="2012-04-18" version="2">MSDNLRLLIFTMTNKDFNETLINLVQPNFHLYDSKYTHYKDSTKKQNTWRYISQQIGQPADACMKRWRSLRDRFGKEKKKMDSLKTSGAGAVNCIVWEHYHHLQFLNDHLRHRLTVGNVQPAVSDETDASAVSMPPSTSYENRDDDDMKGLNIEAVFMKEEPPAQQARTHLELADDSECSSPAEPAISQHQKRKQDLSFHMQEQLISILEKNANTFQKLVSQNVPTEDMFDHFGKVIAAKLRNMPTEASDEIMIAMMQLFTTHKNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>F7B2R9</accession>
    <name>F7B2R9_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Nondiscriminating glutamyl-tRNA synthetase EARS2, mitochondrial</fullName>
            <ecNumber evidence="3">6.1.1.17</ecNumber>
            <ecNumber evidence="10">6.1.1.24</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Glutamate--tRNA(Gln) ligase EARS2, mitochondrial</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Glutamyl-tRNA synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Mitochondrial glutamyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">wild type</strain>
        </source>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>tRNA(Gln) + L-glutamate + ATP = L-glutamyl-tRNA(Gln) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:64612"/>
            <dbReference type="Rhea" id="RHEA-COMP:9662"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:64613"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>tRNA(Glu) + L-glutamate + ATP = L-glutamyl-tRNA(Glu) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23540"/>
            <dbReference type="Rhea" id="RHEA-COMP:9663"/>
            <dbReference type="Rhea" id="RHEA-COMP:9680"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.17"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:23541"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>tRNA(Glx) + L-glutamate + ATP = L-glutamyl-tRNA(Glx) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:18397"/>
            <dbReference type="Rhea" id="RHEA-COMP:9713"/>
            <dbReference type="Rhea" id="RHEA-COMP:9716"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.24"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:18398"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.17" evidence="3"/>
    <dbReference type="EC" id="6.1.1.24" evidence="10"/>
    <dbReference type="EMBL" id="EAAA01002955">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F7B2R9"/>
    <dbReference type="FunCoup" id="F7B2R9">
        <property type="interactions" value="81"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000005341"/>
    <dbReference type="Ensembl" id="ENSCINT00000005341.3">
        <property type="protein sequence ID" value="ENSCINP00000005341.3"/>
        <property type="gene ID" value="ENSCING00000002621.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000009759"/>
    <dbReference type="HOGENOM" id="CLU_015768_6_3_1"/>
    <dbReference type="InParanoid" id="F7B2R9"/>
    <dbReference type="OMA" id="LNFMAYY"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004818">
        <property type="term" value="F:glutamate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050561">
        <property type="term" value="F:glutamate-tRNA(Gln) ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006424">
        <property type="term" value="P:glutamyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00808">
        <property type="entry name" value="GluRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000045">
        <property type="entry name" value="Glutamate--tRNA ligase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00022">
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        <source>
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        <source>
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    </evidence>
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        <source>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        </source>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">wild type</strain>
        </source>
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    <reference evidence="1" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="component" value="Chromosome 8"/>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000027209.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="134" mass="13944" checksum="D74FEF8F1B4327A7" modified="2012-04-18" version="2">MRPIGGGTGGPTSGTGGFGFGRLGGALSPAMRQCMQGCATSNACLTRSQAWSAIRSTTTGCISCVRQQCGTTQPQQMNEISSCVISARINCPSCPRPSTVEQMQMQQCRTCLVNCVRSSSGLASAGLDTLRKWG</sequence>
</entry>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
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                <person name="Satake M."/>
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                <person name="Shin-i T."/>
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                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
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                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
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                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    <dbReference type="Ensembl" id="ENSCINT00000028726.2">
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        <property type="gene ID" value="ENSCING00000016436.2"/>
    </dbReference>
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    <dbReference type="InParanoid" id="F7BCA2"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003354162" evidence="1">
        <location>
            <begin position="24"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000028480.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="66" mass="7990" checksum="78F1045DC143C669" modified="2012-04-18" version="2">MNKSALILLLLIGLLVLTEPTVGLFGRRRRRCWRRRRRYGDEQAMKDRHGVPRNGRRRYWVKQLIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>H2XKW2</accession>
    <name>H2XKW2_CIOIN</name>
    <protein>
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            <fullName evidence="8">5-taurinomethyluridine-[tRNA] synthase subunit MTO1, mitochondrial</fullName>
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            <fullName evidence="9">Mitochondrial tRNA translation optimization 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Protein MTO1 homolog, mitochondrial</fullName>
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    <organism evidence="12 13">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">wild type</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the GTPBP3-MTO1 complex that catalyzes the 5-taurinomethyluridine (taum(5)U) modification at the 34th wobble position (U34) of mitochondrial tRNAs (mt-tRNAs), which plays a role in mt-tRNA decoding and mitochondrial translation. Taum(5)U formation on mammalian mt-tRNA requires the presence of both GTPBP3-mediated GTPase activity and MTO1 catalytic activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>5,10-methylenetetrahydrofolate + uridine(34) in tRNA + taurine + GTP + A + H2O = 5-taurinomethyluridine(34) in tRNA + 7,8-dihydrofolate + GDP + AH2 + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:83279"/>
            <dbReference type="Rhea" id="RHEA-COMP:11727"/>
            <dbReference type="Rhea" id="RHEA-COMP:11732"/>
            <dbReference type="ChEBI" id="CHEBI:12071"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57451"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:87172"/>
            <dbReference type="ChEBI" id="CHEBI:507393"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:83280"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer; forms a dimer in the presence of potassium. Interacts with GTPBP3; forms the GTPBP3-MTO1 complex composed of homodimers of GTPBP3 and MTO1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MnmG family.</text>
    </comment>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XKW2"/>
    <dbReference type="FunCoup" id="H2XKW2">
        <property type="interactions" value="551"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000030294"/>
    <dbReference type="Ensembl" id="ENSCINT00000036990.1">
        <property type="protein sequence ID" value="ENSCINP00000030294.1"/>
        <property type="gene ID" value="ENSCING00000021576.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000011297"/>
    <dbReference type="HOGENOM" id="CLU_007831_2_1_1"/>
    <dbReference type="InParanoid" id="H2XKW2"/>
    <dbReference type="OMA" id="CNPAMGG"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070899">
        <property type="term" value="P:mitochondrial tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000082">
        <property type="entry name" value="protein MTO1 homolog, mitochondrial isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.570:FF:000001">
        <property type="entry name" value="tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG"/>
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    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000002">
        <property type="entry name" value="tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.570">
        <property type="entry name" value="GidA associated domain, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00129">
        <property type="entry name" value="MnmG_GidA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004416">
        <property type="entry name" value="MnmG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002218">
        <property type="entry name" value="MnmG-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020595">
        <property type="entry name" value="MnmG-rel_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026904">
        <property type="entry name" value="MnmG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047001">
        <property type="entry name" value="MnmG_C_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044920">
        <property type="entry name" value="MnmG_C_subdom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040131">
        <property type="entry name" value="MnmG_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00136">
        <property type="entry name" value="mnmG_gidA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11806">
        <property type="entry name" value="GLUCOSE INHIBITED DIVISION PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11806:SF0">
        <property type="entry name" value="PROTEIN MTO1 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01134">
        <property type="entry name" value="GIDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13932">
        <property type="entry name" value="SAM_GIDA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01228">
        <property type="entry name" value="GIDA_assoc_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01280">
        <property type="entry name" value="GIDA_1"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS01281">
        <property type="entry name" value="GIDA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="tRNA uridine 5-carboxymethylaminomethyl modification enzyme C-terminal subdomain" evidence="11">
        <location>
            <begin position="556"/>
            <end position="627"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00051247"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00056538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00061980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00068446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00079308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00083348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000030294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="637" mass="70426" checksum="E25128E4C3DB5CBC" modified="2012-04-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>H2XL98</accession>
    <name>H2XL98_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Fibrous sheath-interacting protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">wild type</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FSIP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01000824">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XL98"/>
    <dbReference type="Ensembl" id="ENSCINT00000031939.1">
        <property type="protein sequence ID" value="ENSCINP00000030430.1"/>
        <property type="gene ID" value="ENSCING00000018774.1"/>
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    <dbReference type="GeneTree" id="ENSGT00390000013879"/>
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    <dbReference type="InParanoid" id="H2XL98"/>
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        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026246">
        <property type="entry name" value="Fsip1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22012">
        <property type="entry name" value="FIBROUS SHEATH INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22012:SF2">
        <property type="entry name" value="FIBROUS SHEATH-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15554">
        <property type="entry name" value="FSIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
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        <location>
            <begin position="191"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="198"/>
            <end position="209"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00010495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019480"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000030430.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">wild type</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XM40"/>
    <dbReference type="Ensembl" id="ENSCINT00000030475.1">
        <property type="protein sequence ID" value="ENSCINP00000030722.1"/>
        <property type="gene ID" value="ENSCING00000018312.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 5"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000030722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="26" mass="3041" checksum="3031D02874F02103" modified="2012-04-18" version="1">MKWCLIFPPPYCILPIKHAISNNVEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>H2XN34</accession>
    <name>H2XN34_CIOIN</name>
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        <recommendedName>
            <fullName evidence="5">GT23 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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            <taxon>Tunicata</taxon>
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    <reference evidence="7" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
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                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
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                <person name="Makabe K.W."/>
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                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
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                <person name="Miura S."/>
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                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
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                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">wild type</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyltransferase 23 family.</text>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XN34"/>
    <dbReference type="Ensembl" id="ENSCINT00000036636.1">
        <property type="protein sequence ID" value="ENSCINP00000031067.1"/>
        <property type="gene ID" value="ENSCING00000019924.1"/>
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        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046921">
        <property type="term" value="F:alpha-(1-&gt;6)-fucosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Fut8_N_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027350">
        <property type="entry name" value="GT23_dom"/>
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    <dbReference type="PANTHER" id="PTHR13132">
        <property type="entry name" value="ALPHA- 1,6 -FUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13132:SF29">
        <property type="entry name" value="ALPHA-(1,6)-FUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
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    <feature type="domain" description="GT23" evidence="5">
        <location>
            <begin position="157"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Important for donor substrate binding" evidence="3">
        <location>
            <begin position="324"/>
            <end position="325"/>
        </location>
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        <source>
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    </evidence>
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        <source>
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        <source>
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    </evidence>
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        <source>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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    </evidence>
    <sequence length="449" mass="52490" checksum="4C60BB8310063B15" modified="2012-04-18" version="1">QLLRGFQHARGLCWRTAYYFVTLFACGCCLFNVIFDMRGTSVIIALQLTGIQNWTYKTAPVTKLDVERTKLTMELKRDVELWWKTIKAELLEFKKSSRKEKNIVFQNINQCLKSRALEIKMENVASFDEEWQKQLSNKTAKNVEKTIDRLQNPKRCDQYGQLYVKVAECGFGCIMHDLTNKLNIATSYNKTMILQTSTSIYTRSQIMSDILEPISKKCQVPIKGKWVEPYIFTFVIPEFSDLTKNKLCFIKRQISGRAYAFRYPVFTDEMWGNVEAFHSDPMGWWISQMLKSLFKPKPKLQERMDYYAENVDFSSPIVGYVHVRRTDKLTDDREFAVFRPLSEFMKHVEEWYDKYDLRQAGLGNNLKVEQRVYLASDDLAVWDEAKIFDKYTFFGNKEFTERASEIKTRDTKSGLEDIIIEATLLSKCNFFVGTLSSEIGQLAIEMKQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>H2XP33</accession>
    <name>H2XP33_CIOIN</name>
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            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">wild type</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="H2XP33"/>
    <dbReference type="STRING" id="7719.ENSCINP00000031416"/>
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        <property type="gene ID" value="ENSCING00000018188.1"/>
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    <dbReference type="HOGENOM" id="CLU_375497_0_0_1"/>
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        <property type="component" value="Chromosome 11"/>
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        <property type="entry name" value="GENE 9195-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GENE, 32742-RELATED-RELATED"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <begin position="255"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="255"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000031416.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>H2XPL6</accession>
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            <fullName evidence="11">Mitochondrial 2-oxodicarboxylate carrier</fullName>
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        <alternativeName>
            <fullName evidence="12">Solute carrier family 25 member 21</fullName>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
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            <taxon>Phlebobranchia</taxon>
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            <taxon>Ciona</taxon>
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    <reference evidence="23" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">wild type</strain>
        </source>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Transports dicarboxylates across the inner membranes of mitochondria by a counter-exchange mechanism. Can transport 2-oxoadipate (2-oxohexanedioate), 2-oxoglutarate, adipate (hexanedioate), glutarate, and to a lesser extent, pimelate (heptanedioate), 2-oxopimelate (2-oxoheptanedioate), 2-aminoadipate (2-aminohexanedioate), oxaloacetate, and citrate. Plays a central role in catabolism of lysine, hydroxylysine, and tryptophan, by transporting common metabolite intermediates (such as 2-oxoadipate) into the mitochondria, where it is converted into acetyl-CoA and can enter the citric acid (TCA) cycle.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>2-oxoadipate(in) + 2-oxoglutarate(out) = 2-oxoadipate(out) + 2-oxoglutarate(in)</text>
            <dbReference type="Rhea" id="RHEA:71739"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:57499"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>2-oxoheptanedioate(in) + 2-oxoglutarate(out) = 2-oxoheptanedioate(out) + 2-oxoglutarate(in)</text>
            <dbReference type="Rhea" id="RHEA:71755"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:72701"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-2-aminoadipate(in) + 2-oxoglutarate(out) = L-2-aminoadipate(out) + 2-oxoglutarate(in)</text>
            <dbReference type="Rhea" id="RHEA:71747"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:58672"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>citrate(in) + 2-oxoglutarate(out) = citrate(out) + 2-oxoglutarate(in)</text>
            <dbReference type="Rhea" id="RHEA:71763"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:16947"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>glutarate(in) + 2-oxoglutarate(out) = glutarate(out) + 2-oxoglutarate(in)</text>
            <dbReference type="Rhea" id="RHEA:71751"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30921"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>heptanedioate(in) + 2-oxoglutarate(out) = heptanedioate(out) + 2-oxoglutarate(in)</text>
            <dbReference type="Rhea" id="RHEA:71759"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:36165"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>hexanedioate(in) + 2-oxoglutarate(out) = hexanedioate(out) + 2-oxoglutarate(in)</text>
            <dbReference type="Rhea" id="RHEA:71743"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:17128"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 21">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="EAAA01000840">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XPL6"/>
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    <dbReference type="Ensembl" id="ENSCINT00000034717.1">
        <property type="protein sequence ID" value="ENSCINP00000031600.1"/>
        <property type="gene ID" value="ENSCING00000019031.1"/>
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    <dbReference type="HOGENOM" id="CLU_015166_5_2_1"/>
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        <property type="component" value="Chromosome 11"/>
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        <property type="evidence" value="ECO:0007669"/>
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        <property type="entry name" value="MCP_transmembrane"/>
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    <dbReference type="InterPro" id="IPR051752">
        <property type="entry name" value="Mito_2-oxodicarb_carrier"/>
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    <dbReference type="PANTHER" id="PTHR46356">
        <property type="entry name" value="MITOCHONDRIAL 2-OXODICARBOXYLATE CARRIER"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR46356:SF1">
        <property type="entry name" value="MITOCHONDRIAL 2-OXODICARBOXYLATE CARRIER"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
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        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
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    <keyword evidence="9 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
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    <keyword evidence="4 20 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 21" id="KW-0813">Transport</keyword>
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        <location>
            <begin position="12"/>
            <end position="100"/>
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    <feature type="repeat" description="Solcar" evidence="20">
        <location>
            <begin position="107"/>
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    <feature type="repeat" description="Solcar" evidence="20">
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            <begin position="206"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
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    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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    <evidence type="ECO:0000256" key="4">
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
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                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="H2XTK6"/>
    <dbReference type="Ensembl" id="ENSCINT00000031181.1">
        <property type="protein sequence ID" value="ENSCINP00000032990.1"/>
        <property type="gene ID" value="ENSCING00000022823.1"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2399008_0_0_1"/>
    <dbReference type="InParanoid" id="H2XTK6"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="57"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000032990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="93" mass="10415" checksum="C638E6E31CCB29C4" modified="2012-04-18" version="1">MATTDTQSSVNETDEQPINTRWVPERATGHSVNQTDLRRDFREMVLATSFRSINSRERAEMSGLHGEATSGKHRNVTMPNNDVIQRSSSIAVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>H2XTZ3</accession>
    <name>H2XTZ3_CIOIN</name>
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        <recommendedName>
            <fullName evidence="9">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Tunicata</taxon>
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            <taxon>Phlebobranchia</taxon>
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            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">wild type</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="EAAA01000386">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XTZ3"/>
    <dbReference type="STRING" id="7719.ENSCINP00000033127"/>
    <dbReference type="Ensembl" id="ENSCINT00000031073.1">
        <property type="protein sequence ID" value="ENSCINP00000033127.1"/>
        <property type="gene ID" value="ENSCING00000019034.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154899"/>
    <dbReference type="HOGENOM" id="CLU_011722_7_0_1"/>
    <dbReference type="InParanoid" id="H2XTZ3"/>
    <dbReference type="OMA" id="ECETRCE"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18378">
        <property type="entry name" value="BTB_POZ_Kv2_KCNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000010">
        <property type="entry name" value="Potassium voltage-gated channel subfamily B member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003971">
        <property type="entry name" value="K_chnl_volt-dep_Kv5/Kv9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
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    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028325">
        <property type="entry name" value="VG_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537:SF254">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL PROTEIN SHAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537">
        <property type="entry name" value="VOLTAGE-GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="KCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01494">
        <property type="entry name" value="KV9CHANNEL"/>
    </dbReference>
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        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0407">Ion channel</keyword>
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    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="180"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="BTB" evidence="9">
        <location>
            <begin position="25"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000033127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="214" mass="25002" checksum="72F80B580DAC252D" modified="2012-04-18" version="1">RRKKSRKFTRCEKGENMEEKQKKRQRVRLNVGGYIHEVRWGTLEQLPHTRLGKLRECETRCEIMGLADDHDPTSNEYFFDRHPGAFTPILNFYRTGKLHMPEDICALAFSAELDYWGIDDIYIESCCQARYHQKKEQILEELRREADAIRERQGDDFRGMCCAERRKLVWDLMEKPNSSLAAKVIAIVSVLFIVLSTVALTLNTVPGLQGKLYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>H2XU31</accession>
    <name>H2XU31_CIOIN</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
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            <taxon>Metazoa</taxon>
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            <taxon>Tunicata</taxon>
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            <taxon>Phlebobranchia</taxon>
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            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
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                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
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                <person name="Huang W."/>
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                <person name="Putnam N."/>
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                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
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            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000033492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="725" mass="77104" checksum="8600F9540D0BC98A" modified="2012-04-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>H2XVM6</accession>
    <accession>A0A1W2WI81</accession>
    <name>H2XVM6_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="9">C-Jun-amino-terminal kinase-interacting protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC100180686</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the JIP scaffold family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_002128105.1">
        <property type="nucleotide sequence ID" value="XM_002128069.5"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XVM6"/>
    <dbReference type="STRING" id="7719.ENSCINP00000033710"/>
    <dbReference type="Ensembl" id="ENSCINT00000034772.1">
        <property type="protein sequence ID" value="ENSCINP00000033710.1"/>
        <property type="gene ID" value="ENSCING00000021680.1"/>
    </dbReference>
    <dbReference type="GeneID" id="100180686"/>
    <dbReference type="KEGG" id="cin:100180686"/>
    <dbReference type="GeneTree" id="ENSGT00940000169182"/>
    <dbReference type="HOGENOM" id="CLU_634512_0_0_1"/>
    <dbReference type="InParanoid" id="H2XVM6"/>
    <dbReference type="OrthoDB" id="5965083at2759"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008432">
        <property type="term" value="F:JUN kinase binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005078">
        <property type="term" value="F:MAP-kinase scaffold activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007254">
        <property type="term" value="P:JNK cascade"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046328">
        <property type="term" value="P:regulation of JNK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01212">
        <property type="entry name" value="PTB_JIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11801">
        <property type="entry name" value="SH3_JIP1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000032">
        <property type="entry name" value="Putative C-Jun-amino-terminal kinase-interacting protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047178">
        <property type="entry name" value="JIP1_scaffold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006020">
        <property type="entry name" value="PTB/PI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47437">
        <property type="entry name" value="JNK-INTERACTING PROTEIN 1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47437:SF4">
        <property type="entry name" value="JNK-INTERACTING PROTEIN 1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00640">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00462">
        <property type="entry name" value="PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01179">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="8">
        <location>
            <begin position="194"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="PID" evidence="7">
        <location>
            <begin position="272"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="42"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="113"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="42"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="123"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS01179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000033710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="432" mass="49779" checksum="7D4F7A6B90AE7DAA" modified="2012-04-18" version="1">MEYGSVNFELSLDDFNDEDLSDIIIDEDSKLHCYVNDNFKVTKRRSQKKKKVSNDASHAPKRPRPQTLRFFAAPLRPGPLPPKIPERVIPQVEDVELKQTHCDVTNNNTINNTKNSDCNQTSEKTKHIREESTKPRFRRSIAVCTKSNTSEDIVNLTSVREQRRHRCPGLTRSASEWSIEDPDKIEYCYMDGELKEQTHSALFKFVPRHKDEIQLEINDPLCVENQADDFWFEGINMRTGERGAFPAYYAQGVGEGCDDVSEFSVREENCEKFHLYFLGSVEVSSHKGNEVLCQAMHEVAQSRRHTLHTSPPAYVCLLVNHHGIKMMDQSLMKKRSTSSCDVNKTFSSSSKVRERIHFFQLRNVSFCGHHPKNDKYFAFITRHPDAPSRFACHTFVSEASTRPLAESVGRAFATFYQEFLECQHPIEDIYIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>H2XXB5</accession>
    <name>H2XXB5_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">wild type</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01000058">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H2XXB5"/>
    <dbReference type="Ensembl" id="ENSCINT00000033869.1">
        <property type="protein sequence ID" value="ENSCINP00000034299.1"/>
        <property type="gene ID" value="ENSCING00000018391.1"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2290661_0_0_1"/>
    <dbReference type="InParanoid" id="H2XXB5"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000034299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="101" mass="10938" checksum="1B052598782B87E1" modified="2012-04-18" version="1">MGQSVVVVKLSHPARHVTLAALDRHLPARTTTSTWAVLQPVITQHSTRSVLADKPLASPTQKIVGSLWIPGRAEYVHGNQTQKGATQIEILLELIKTDGAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>H2XXJ2</accession>
    <name>H2XXJ2_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    <dbReference type="Ensembl" id="ENSCINT00000036811.1">
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        <property type="gene ID" value="ENSCING00000022891.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00730000111039"/>
    <dbReference type="HOGENOM" id="CLU_1673173_0_0_1"/>
    <dbReference type="InParanoid" id="H2XXJ2"/>
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    <proteinExistence type="predicted"/>
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    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000034376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="158" mass="18497" checksum="DDECAC8C37AC8AAF" modified="2012-04-18" version="1">TSSTPHPDTCEAEQPDLAAVKDQLERLEAKLDSRSEVDTTEVHRINFANIKVEFENITLRREVSVLRKVQEVVEVGTTTCHRCSLMEEDNEKLHRDIERLKLELGHFEKYFFDEVEDLKFNYKQAVKTSLLYEGQLKSISHKYHIKVDVLTHNDVERL</sequence>
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<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>H2XXU0</accession>
    <name>H2XXU0_CIOIN</name>
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        <recommendedName>
            <fullName evidence="11">Kinesin light chain</fullName>
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    </protein>
    <organism evidence="14 15">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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            <taxon>Phlebobranchia</taxon>
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            <taxon>Ciona</taxon>
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    <reference evidence="15" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Kinesin is a microtubule-associated force-producing protein that play a role in organelle transport.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Oligomeric complex composed of two heavy chains and two light chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cytoplasm</location>
            <location evidence="1 11">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the kinesin light chain family.</text>
    </comment>
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    <dbReference type="FunCoup" id="H2XXU0">
        <property type="interactions" value="7"/>
    </dbReference>
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    <dbReference type="Ensembl" id="ENSCINT00000036418.1">
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        <property type="gene ID" value="ENSCING00000018744.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155555"/>
    <dbReference type="HOGENOM" id="CLU_019953_0_0_1"/>
    <dbReference type="InParanoid" id="H2XXU0"/>
    <dbReference type="OMA" id="EQDDTCE"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0005871">
        <property type="term" value="C:kinesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="F:kinesin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="P:anterior commissure morphogenesis"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="kinesin light chain isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002151">
        <property type="entry name" value="Kinesin_light"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015792">
        <property type="entry name" value="Kinesin_light_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45783">
        <property type="entry name" value="KINESIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45783:SF3">
        <property type="entry name" value="KINESIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13176">
        <property type="entry name" value="TPR_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00381">
        <property type="entry name" value="KINESINLIGHT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01160">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4 11" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8 11" id="KW-0505">Motor protein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 10" id="KW-0802">TPR repeat</keyword>
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        <location>
            <begin position="301"/>
            <end position="334"/>
        </location>
    </feature>
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        <location>
            <begin position="343"/>
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    </feature>
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            <begin position="385"/>
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    <feature type="region of interest" description="Disordered" evidence="13">
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    <feature type="compositionally biased region" description="Polar residues" evidence="13">
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        <location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
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        <source>
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    </evidence>
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        <source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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        <source>
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        <source>
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    </evidence>
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        <source>
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        <source>
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        <source>
            <dbReference type="RuleBase" id="RU367020"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000034474.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="597" mass="66315" checksum="6933328AAE136A0C" modified="2012-04-18" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>H2XXW1</accession>
    <name>H2XXW1_CIOIN</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
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        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
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        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
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                <person name="Doyle S."/>
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                <person name="Hayashi H."/>
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                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
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                <person name="Makabe K.W."/>
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                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
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                <person name="Nomoto H."/>
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                <person name="Oishi K."/>
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                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
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                <person name="Suzuki M.M."/>
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                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
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                <person name="Detter C."/>
                <person name="Doggett N."/>
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                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="5">wild type</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Gelpke M."/>
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                <person name="Hastings K.E."/>
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                <person name="Kawashima T."/>
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                <person name="Putnam N."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Satake M."/>
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                <person name="Tassy O."/>
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                <person name="Yagi K."/>
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                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Ueno K."/>
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                <person name="Matsumoto J."/>
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                <person name="Macmil S.L."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>IDENTIFICATION</scope>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
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                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
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                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
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                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
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                <person name="Kobayashi K."/>
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                <person name="Mochizuki Y."/>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Hastings K.E."/>
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                <person name="Meinertzhagen I.A."/>
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                <person name="Wang H.G."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
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                <person name="Yamada L."/>
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                <person name="Wiley G.B."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="10">wild type</strain>
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            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">wild type</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the sulfotransferase 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="3"/>
    <dbReference type="EMBL" id="EAAA01002997">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="L7N0U7"/>
    <dbReference type="Ensembl" id="ENSCINT00000024129.2">
        <property type="protein sequence ID" value="ENSCINP00000023883.2"/>
        <property type="gene ID" value="ENSCING00000012899.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163815"/>
    <dbReference type="HOGENOM" id="CLU_027239_1_2_1"/>
    <dbReference type="InParanoid" id="L7N0U7"/>
    <dbReference type="OMA" id="ESACEYK"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051923">
        <property type="term" value="P:sulfation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11783">
        <property type="entry name" value="SULFOTRANSFERASE SULT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Sulfotransferase" evidence="4">
        <location>
            <begin position="16"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000023883.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="271" mass="31741" checksum="E587FC8B52E6DBED" modified="2013-03-06" version="1">TQEIFDSAQAYEPDSSDIFVVTYPKCGTTWMLNILYLIEHGGNMIGSDDNLNYHYPFLELVGGDKVQEFKAPRVIKTHLDDRMIKHKEGVKYIFVARNPKDCVVSFFHHTLDFHNFYKYKGGKFDDYFKIFMQGKVDWGDYFVMVPRWYEKSLKEKNVHFVLYEDMKIDPKSEIEKLANFLGPKWSDSIKREGVLENVIKNSSFNSMKSNHLNQIIKLQQKTKTSSFRLQCITIAPKDLKFMRKGAAGDWRNTLSEEQSQEIDEKMKKVGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-16" modified="2026-01-28" version="114" xmlns="https://uniprot.org/uniprot">
    <accession>Q1RL48</accession>
    <accession>F6Q2D1</accession>
    <name>Q1RL48_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">Ci-ZF(ZZ)-7</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="journal article" date="2006" name="Dev. Biol." volume="292" first="546" last="554">
            <title>Systematic analysis of embryonic expression profiles of zinc finger genes in Ciona intestinalis.</title>
            <authorList>
                <person name="Miwata K."/>
                <person name="Chiba T."/>
                <person name="Horii R."/>
                <person name="Yamada L."/>
                <person name="Kubo A."/>
                <person name="Miyamura D."/>
                <person name="Satoh N."/>
                <person name="Satou Y."/>
            </authorList>
            <dbReference type="PubMed" id="16519883"/>
            <dbReference type="DOI" id="10.1016/j.ydbio.2006.01.024"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">wild type</strain>
        </source>
    </reference>
    <reference evidence="18" key="4">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="EMBL" id="EAAA01000363">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="BR000186">
        <property type="protein sequence ID" value="FAA00217.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1RL48"/>
    <dbReference type="STRING" id="7719.ENSCINP00000001354"/>
    <dbReference type="Ensembl" id="ENSCINT00000001354.3">
        <property type="protein sequence ID" value="ENSCINP00000001354.3"/>
        <property type="gene ID" value="ENSCING00000000739.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156781"/>
    <dbReference type="HOGENOM" id="CLU_007287_2_1_1"/>
    <dbReference type="OMA" id="RHNADME"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007219">
        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02339">
        <property type="entry name" value="ZZ_Mind_bomb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000090">
        <property type="entry name" value="E3 ubiquitin-protein ligase MIB1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000113">
        <property type="entry name" value="E3 ubiquitin-protein ligase mib1 isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000259">
        <property type="entry name" value="E3 ubiquitin-protein ligase mind-bomb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.90:FF:000005">
        <property type="entry name" value="Putative E3 ubiquitin-protein ligase mib1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000054">
        <property type="entry name" value="Putative e3 ubiquitin-protein ligase mind-bomb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042056">
        <property type="entry name" value="MIB1/2_ZZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010606">
        <property type="entry name" value="Mib_Herc2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037252">
        <property type="entry name" value="Mib_Herc2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040847">
        <property type="entry name" value="SH3_15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000433">
        <property type="entry name" value="Znf_ZZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043145">
        <property type="entry name" value="Znf_ZZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24202:SF53">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE MIB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24202">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE MIB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06701">
        <property type="entry name" value="MIB_HERC2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18346">
        <property type="entry name" value="SH3_15"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00569">
        <property type="entry name" value="ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00291">
        <property type="entry name" value="ZnF_ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159034">
        <property type="entry name" value="Mib/herc2 domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51416">
        <property type="entry name" value="MIB_HERC2"/>
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</entry>
<entry dataset="TrEMBL" created="2003-03-01" modified="2026-01-28" version="127" xmlns="https://uniprot.org/uniprot">
    <accession>Q8HIM9</accession>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
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            <taxon>Phlebobranchia</taxon>
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        <citation type="journal article" date="2004" name="J. Mol. Evol." volume="58" first="376" last="389">
            <title>Complete mtDNA of Ciona intestinalis reveals extensive gene rearrangement and the presence of an atp8 and an extra trnM gene in ascidians.</title>
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                <person name="Pesole G."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="journal article" date="2015" name="J. Zool. Syst. Evol. Res." volume="53" first="185" last="257">
            <title>Morphological evidence that the molecularly determined Ciona robusta type A and type B are different species: Ciona robusta and Ciona robusta.</title>
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                <person name="Gissi C."/>
                <person name="Pennati R."/>
                <person name="Caicci F."/>
                <person name="Gasparini F."/>
                <person name="Manni L."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <citation type="journal article" date="2015" name="PLoS ONE" volume="10" first="e0122879" last="e0122879">
            <title>Morphological Differences between Larvae of the Ciona intestinalis Species Complex: Hints for a Valid Taxonomic Definition of Distinct Species.</title>
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                <person name="Ficetola G.F."/>
                <person name="Brunetti R."/>
                <person name="Caicci F."/>
                <person name="Gasparini F."/>
                <person name="Griggio F."/>
                <person name="Sato A."/>
                <person name="Stach T."/>
                <person name="Kaul-Strehlow S."/>
                <person name="Gissi C."/>
                <person name="Manni L."/>
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        <citation type="submission" date="2025-05" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <text evidence="21">Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.</text>
    </comment>
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        <reaction evidence="21">
            <text>4 Fe(II)-[cytochrome c] + O2 + 8 H(+)(in) = 4 Fe(III)-[cytochrome c] + 2 H2O + 4 H(+)(out)</text>
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            <dbReference type="Rhea" id="RHEA-COMP:10350"/>
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            <dbReference type="ChEBI" id="CHEBI:15377"/>
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            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
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        </reaction>
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        <cofactor evidence="1">
            <name>Cu cation</name>
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            <name>heme</name>
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    </comment>
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        <text evidence="4 21">Energy metabolism; oxidative phosphorylation.</text>
    </comment>
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        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
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            <topology evidence="21">Multi-pass membrane protein</topology>
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        <text evidence="5 21">Belongs to the heme-copper respiratory oxidase family.</text>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
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                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
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                <person name="Inaba K."/>
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            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="6">wild type</strain>
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    <reference evidence="6" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS19 family.</text>
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        <property type="term" value="F:structural constituent of ribosome"/>
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        <property type="project" value="Ensembl"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00035466"/>
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            <dbReference type="Ensembl" id="ENSCINP00000028979.2"/>
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        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
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                <person name="Kobayashi M."/>
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                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
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                <person name="Mochizuki Y."/>
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                <person name="Oishi K."/>
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                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">wild type</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="13" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
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    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SEC62 family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
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                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
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                <person name="Doyle S."/>
                <person name="Francino P."/>
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                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
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                <person name="Inaba K."/>
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                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
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                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
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                <person name="Lucas S."/>
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</entry>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
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                <person name="Huang W."/>
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            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
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                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
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                <person name="Putnam N."/>
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                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
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                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
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                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
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                <person name="Nishizaka S."/>
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                <person name="Ohta F."/>
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                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
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                <person name="Levine M."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <source>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
                <consortium name="Ensembl"/>
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            <authorList>
                <consortium name="Ensembl"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
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        <name type="common">Transparent sea squirt</name>
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        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="22">wild type</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
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        <text evidence="2">Belongs to the potassium channel family. Calcium-activated (TC 1.A.1.3) subfamily. KCa1.1/KCNMA1 sub-subfamily.</text>
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        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <feature type="domain" description="RCK N-terminal" evidence="21">
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00033447"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS51201"/>
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        <source>
            <dbReference type="Ensembl" id="ENSCINP00000004912.3"/>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
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        <name evidence="8" type="primary">LOC100179636</name>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
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            <taxon>Ciona</taxon>
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    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
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    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
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    <feature type="transmembrane region" description="Helical" evidence="6">
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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        <source>
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        <source>
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    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000028812.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="208" mass="23659" checksum="0A2B54ED4C3025EA" modified="2011-07-27" version="1">MVAEENPTGFRYLTTFQALFQIVELLLLMVVWALVASQKFLFIPGFAFSLGAFVTIWLITLLFFVLYIFGAHVKINQSLAKQNANTEEPSTSGTAGSLKPPPEVRLKIKKVVWFVTEYVIYFFCIIFLFIAVCIVTSKAYTNQHFIAAYAIGWIVLGFYILHFCLLVRNTEGKWPHQCLMECCGLIRKRKTEDTSQLLPTEQASPDNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>F6RG14</accession>
    <name>F6RG14_CIOIN</name>
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        <recommendedName>
            <fullName evidence="3">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
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            <taxon>Ciona</taxon>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">wild type</strain>
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            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <text evidence="1">Belongs to the synaptotagmin family.</text>
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        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
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                <person name="Hastings K.E."/>
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                <person name="Meinertzhagen I.A."/>
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                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
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                <person name="Branno M."/>
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                <person name="Hayashi H."/>
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                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
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                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
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                <person name="Detter C."/>
                <person name="Doggett N."/>
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                <person name="Hawkins T."/>
                <person name="Richardson P."/>
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    <reference evidence="5" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Ueno K."/>
                <person name="Yamada L."/>
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                <person name="Wasserscheid J."/>
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                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
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            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">wild type</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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    </evidence>
    <sequence length="416" mass="45942" checksum="11173BAB35376E96" modified="2012-04-18" version="2">MFDYSAENDDELTLVKGDIVIILDKVSQDVGWWEGEIVDENGKRRKGVFPDNFVTPLPTVDEDTSSEETSSKDSLDIKTETKATKQKTVVEEEKVVSSKTKTPPLTSKSTNSLKRPAPQVPPIASQSNKPETSYDETDSILPNAHEEPKLTHYKRPGGPKNRRKPDTTGRRERAKESAEEMLPVPDTPTETEPVDLEPTEPTPPPKKDTKRGIQVMPVLGAEGGTGDTPSWLKNLKHRSSQKKPTSGMAVSPNKEEEPAQETPNWRNNLKKTTKQPLQPPKPTPQATSPKPVTSPMVPKQSAIVTSPHTNHAPLSPKEEEKSPHWKSVRSKPDPDTHQNETEVDKPSKVIDAKKPIKPMIPFQPKMPDPSAKKAPPSAAKPKPPPPKSKPMIPPSKPVTSNASMEEMKEDIKLLKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>F6SHX3</accession>
    <name>F6SHX3_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">wild type</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="EAAA01001580">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6SHX3"/>
    <dbReference type="FunCoup" id="F6SHX3">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000012169"/>
    <dbReference type="Ensembl" id="ENSCINT00000012169.3">
        <property type="protein sequence ID" value="ENSCINP00000012169.3"/>
        <property type="gene ID" value="ENSCING00000005899.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000167322"/>
    <dbReference type="HOGENOM" id="CLU_720688_0_0_1"/>
    <dbReference type="InParanoid" id="F6SHX3"/>
    <dbReference type="OMA" id="TWICAIS"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071880">
        <property type="term" value="P:adenylate cyclase-activating adrenergic receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043410">
        <property type="term" value="P:positive regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:001072">
        <property type="entry name" value="lysophosphatidic acid receptor 1-B-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22750">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="48"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="80"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="113"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="156"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="212"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="277"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="309"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="7">
        <location>
            <begin position="60"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000012169.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="335" mass="37335" checksum="CDF4BDEBD04D8D93" modified="2012-04-18" version="2">CTSRAHFDLKSSNIAVSFVNYVVNQSASNMENITFIGSEPRSTSPVEIATSIACILAIFVNFCVLLVIYIGPRAFRTPTFFFISSLATADLLTGITILLAIFLPVSDHSWERIVLKGLAVVCFSASVNNLMVIAGDRYLKINDDRRYGTVFTNRSAAILIVLTWICAISLFLVAPLVGWSCGEDFCTCPRDAMCVCDKNYCSQSFTPFSKSYLIVGVSYFVVSLIAMVGLYWAIFASVRRRAGSKRHRNGSVVGPVASRGPFVMIHRRREIRLAKTLVIVMGVFFICWLPVVTLFVYDVIVDDKRLIVWFDYCLASAVIHPLANPIIYSLRLPRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>F6SSS9</accession>
    <name>F6SSS9_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">isopentenyl-diphosphate Delta-isomerase</fullName>
            <ecNumber evidence="5">5.3.3.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">wild type</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the 1,3-allylic rearrangement of the homoallylic substrate isopentenyl (IPP) to its highly electrophilic allylic isomer, dimethylallyl diphosphate (DMAPP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>isopentenyl diphosphate = dimethylallyl diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23284"/>
            <dbReference type="ChEBI" id="CHEBI:57623"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="EC" id="5.3.3.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from isopentenyl diphosphate: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the IPP isomerase type 1 family.</text>
    </comment>
    <dbReference type="EC" id="5.3.3.2" evidence="5"/>
    <dbReference type="EMBL" id="EAAA01002651">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6SSS9"/>
    <dbReference type="FunCoup" id="F6SSS9">
        <property type="interactions" value="227"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000004433"/>
    <dbReference type="Ensembl" id="ENSCINT00000004433.3">
        <property type="protein sequence ID" value="ENSCINP00000004433.3"/>
        <property type="gene ID" value="ENSCING00000002165.3"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000008527"/>
    <dbReference type="HOGENOM" id="CLU_060552_0_1_1"/>
    <dbReference type="InParanoid" id="F6SSS9"/>
    <dbReference type="OMA" id="KAPFDNG"/>
    <dbReference type="UniPathway" id="UPA00059">
        <property type="reaction ID" value="UER00104"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004452">
        <property type="term" value="F:isopentenyl-diphosphate delta-isomerase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050992">
        <property type="term" value="P:dimethylallyl diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009240">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02885">
        <property type="entry name" value="NUDIX_IPP_Isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.79.10:FF:000096">
        <property type="entry name" value="Isopentenyl-diphosphate Delta-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011876">
        <property type="entry name" value="IsopentenylPP_isomerase_typ1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02150">
        <property type="entry name" value="IPP_isom_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10885">
        <property type="entry name" value="ISOPENTENYL-DIPHOSPHATE DELTA-ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10885:SF0">
        <property type="entry name" value="ISOPENTENYL-DIPHOSPHATE DELTA-ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018427">
        <property type="entry name" value="Isopntndiph_ism"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="8">
        <location>
            <begin position="75"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000004433.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="238" mass="27241" checksum="4B8F86757039E4A6" modified="2012-04-18" version="2">MLNTTCIANLSKFVVSHLKNPFSTSGRMADSVDLNTYDTSQVKLMKEECIEVDSDDNVIGPRSKLDCHLLSNGPPLHRAFSLFLFNSKNELLMQKRANAKITFPGYYTNTCCSHPLHNATELEEKESYGVRHAAQRRLKDELGVPINQLPICDISFITRIHYKASCSDKWGEHEIDHVLFARKDVDLNVNPNEVSEVRYVSKDGMKDFTKKCENAGVPLTPWFKLILHSHLFKWWDNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>F6SUL1</accession>
    <name>F6SUL1_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC100181877</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">wild type</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="EAAA01002372">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F6SUL1"/>
    <dbReference type="FunCoup" id="F6SUL1">
        <property type="interactions" value="16"/>
    </dbReference>
    <dbReference type="STRING" id="7719.ENSCINP00000004186"/>
    <dbReference type="Ensembl" id="ENSCINT00000004186.3">
        <property type="protein sequence ID" value="ENSCINP00000004186.3"/>
        <property type="gene ID" value="ENSCING00000012019.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159476"/>
    <dbReference type="HOGENOM" id="CLU_000288_63_0_1"/>
    <dbReference type="InParanoid" id="F6SUL1"/>
    <dbReference type="OMA" id="NEPKFMT"/>
    <dbReference type="Proteomes" id="UP000008144">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034504">
        <property type="term" value="P:protein localization to nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14095">
        <property type="entry name" value="STKc_DCKL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000003">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000066">
        <property type="entry name" value="Serine/threonine-protein kinase DCLK1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="10"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000004186.3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="307" mass="35025" checksum="F0178681A15E6C72" modified="2012-04-18" version="2">MARSNIEARYEIGKTIGDGNFAVVKECRLRNTESEFAMKIIDKSKLKGKEDMIENEIAIMKNCHHPNIVRLIEEFETENEIYLVLEYVKGGDLFDAITESVKFTERDAANMVADLSEALAFLHSKNIIHRDLKPENLLVSRNKDGSMTLKLADFGLAMEVTEPIYTVCGTPTYVAPEILAETGYGLEVDMWATGVITYILLCGFPPFRSLERDQEELFEIIQLGDYEFLSPYWDNISEAAKDLIQRLLVVDTKRRYTAEQVLSHPWIQSEGTYKGPNLQREITMNLEKNFGDRNRRKAGFTQPAAVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>F6SWW8</accession>
    <name>F6SWW8_CIOIN</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
        <dbReference type="NCBI Taxonomy" id="7719"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
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                <person name="Satou Y."/>
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                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
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                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
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                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
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                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
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                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
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                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
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        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
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            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <topology evidence="1">Multi-pass membrane protein</topology>
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        <text evidence="2 6">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
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</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
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            <taxon>Phlebobranchia</taxon>
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    <reference evidence="17" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="DOI" id="10.1126/science.1080049"/>
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    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
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                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">wild type</strain>
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    <reference evidence="16" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
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        </citation>
        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="entry name" value="Heat shock protein 70kD (HSP70), C-terminal subdomain"/>
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        <property type="entry name" value="Heat shock protein 70kD (HSP70), peptide-binding domain"/>
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    <proteinExistence type="inferred from homology"/>
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        <location>
            <begin position="1"/>
            <end position="23"/>
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        <location>
            <begin position="280"/>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00029695"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00030223"/>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00031291"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00033037"/>
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        <source>
            <dbReference type="RuleBase" id="RU003322"/>
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        <source>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="SAM" id="SignalP"/>
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        <source>
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        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="659" mass="72300" checksum="E5032B05F77EF5F8" modified="2012-04-18" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>F6TER2</accession>
    <name>F6TER2_CIOIN</name>
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        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
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        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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            <taxon>Eukaryota</taxon>
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        </lineage>
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    <reference evidence="7" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">wild type</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="EAAA01001734">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="7719.ENSCINP00000023589"/>
    <dbReference type="Ensembl" id="ENSCINT00000023835.2">
        <property type="protein sequence ID" value="ENSCINP00000023589.2"/>
        <property type="gene ID" value="ENSCING00000012709.2"/>
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    <dbReference type="OMA" id="FRIWTER"/>
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        <property type="component" value="Chromosome 3"/>
    </dbReference>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
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        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF288">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TYRKINASE"/>
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        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Protein kinase-like (PK-like)"/>
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    </dbReference>
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        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
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    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="1"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000023589.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008144"/>
        </source>
    </evidence>
    <sequence length="206" mass="23537" checksum="E827279299DA71B3" modified="2012-04-18" version="2">TKESEKLEFSQLLSWSRDIGLGMNYLHEEAPVAVIHRDLKSKNVVICSDFTLKICDFGASRFFGETIVMTITGTYPWMAPELIQGLPTSELCDVYSFGVVLWEMLTREVPFKGLQGFQVAWVVVENGERPTIPECCPGRFSSLMRRCWSSTVSERPSFYDIIGELNSMMSDDDLGATTNHFLGSKEEWKSDIEEKRKELQEKEKEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>F6TN99</accession>
    <name>F6TN99_CIOIN</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Gamma-secretase subunit Aph-1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
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            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
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            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="8" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="8">wild type</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="8" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
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    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the APH-1 family.</text>
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    <dbReference type="EMBL" id="EAAA01001186">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="Ensembl" id="ENSCINT00000005808.3">
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    <dbReference type="GeneTree" id="ENSGT00390000002049"/>
    <dbReference type="HOGENOM" id="CLU_086389_0_0_1"/>
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    <dbReference type="PANTHER" id="PTHR12889">
        <property type="entry name" value="GAMMA-SECRETASE SUBUNIT APH-1"/>
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    <dbReference type="Pfam" id="PF06105">
        <property type="entry name" value="Aph-1"/>
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    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
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    <feature type="transmembrane region" description="Helical" evidence="7">
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    <feature type="transmembrane region" description="Helical" evidence="7">
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    <feature type="transmembrane region" description="Helical" evidence="7">
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    <feature type="transmembrane region" description="Helical" evidence="7">
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    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
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            <end position="224"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022976"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSCINP00000005808.3"/>
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            <dbReference type="Proteomes" id="UP000008144"/>
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    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    </evidence>
    <sequence length="238" mass="26161" checksum="08EEA7FD2DB620F1" modified="2012-04-18" version="2">MPLMVFFGCTMIAFGPSLAMFILTIAKYPLKIILLVVGAFCWLLALLFSALLWLAVVPLKEQLAFSLVFSVIFQEGMRYGLFRMMKNAENGLDDALSEDEKKSISNHKLSYTTGFGFGLMYGLFSIVNVLSQSIGPGSVGIDGHSSNFMLISAFLTSSTILLHTFWNVIVFDALNKQQYYKVITVVCLHMLVSCCSLLNVQQNLAYVSVIVSYIVLIGTTVWAFKAAGGSVVNIKSCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-07-27" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>F6U019</accession>
    <accession>A0A1W2W2T0</accession>
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            <fullName evidence="7 11">Ubiquitin carboxyl-terminal hydrolase</fullName>
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    <gene>
        <name evidence="14" type="primary">LOC100175935</name>
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    <organism evidence="14 15">
        <name type="scientific">Ciona intestinalis</name>
        <name type="common">Transparent sea squirt</name>
        <name type="synonym">Ascidia intestinalis</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Tunicata</taxon>
            <taxon>Ascidiacea</taxon>
            <taxon>Phlebobranchia</taxon>
            <taxon>Cionidae</taxon>
            <taxon>Ciona</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2002" name="Science" volume="298" first="2157" last="2167">
            <title>The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.</title>
            <authorList>
                <person name="Dehal P."/>
                <person name="Satou Y."/>
                <person name="Campbell R.K."/>
                <person name="Chapman J."/>
                <person name="Degnan B."/>
                <person name="De Tomaso A."/>
                <person name="Davidson B."/>
                <person name="Di Gregorio A."/>
                <person name="Gelpke M."/>
                <person name="Goodstein D.M."/>
                <person name="Harafuji N."/>
                <person name="Hastings K.E."/>
                <person name="Ho I."/>
                <person name="Hotta K."/>
                <person name="Huang W."/>
                <person name="Kawashima T."/>
                <person name="Lemaire P."/>
                <person name="Martinez D."/>
                <person name="Meinertzhagen I.A."/>
                <person name="Necula S."/>
                <person name="Nonaka M."/>
                <person name="Putnam N."/>
                <person name="Rash S."/>
                <person name="Saiga H."/>
                <person name="Satake M."/>
                <person name="Terry A."/>
                <person name="Yamada L."/>
                <person name="Wang H.G."/>
                <person name="Awazu S."/>
                <person name="Azumi K."/>
                <person name="Boore J."/>
                <person name="Branno M."/>
                <person name="Chin-Bow S."/>
                <person name="DeSantis R."/>
                <person name="Doyle S."/>
                <person name="Francino P."/>
                <person name="Keys D.N."/>
                <person name="Haga S."/>
                <person name="Hayashi H."/>
                <person name="Hino K."/>
                <person name="Imai K.S."/>
                <person name="Inaba K."/>
                <person name="Kano S."/>
                <person name="Kobayashi K."/>
                <person name="Kobayashi M."/>
                <person name="Lee B.I."/>
                <person name="Makabe K.W."/>
                <person name="Manohar C."/>
                <person name="Matassi G."/>
                <person name="Medina M."/>
                <person name="Mochizuki Y."/>
                <person name="Mount S."/>
                <person name="Morishita T."/>
                <person name="Miura S."/>
                <person name="Nakayama A."/>
                <person name="Nishizaka S."/>
                <person name="Nomoto H."/>
                <person name="Ohta F."/>
                <person name="Oishi K."/>
                <person name="Rigoutsos I."/>
                <person name="Sano M."/>
                <person name="Sasaki A."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Shin-i T."/>
                <person name="Spagnuolo A."/>
                <person name="Stainier D."/>
                <person name="Suzuki M.M."/>
                <person name="Tassy O."/>
                <person name="Takatori N."/>
                <person name="Tokuoka M."/>
                <person name="Yagi K."/>
                <person name="Yoshizaki F."/>
                <person name="Wada S."/>
                <person name="Zhang C."/>
                <person name="Hyatt P.D."/>
                <person name="Larimer F."/>
                <person name="Detter C."/>
                <person name="Doggett N."/>
                <person name="Glavina T."/>
                <person name="Hawkins T."/>
                <person name="Richardson P."/>
                <person name="Lucas S."/>
                <person name="Kohara Y."/>
                <person name="Levine M."/>
                <person name="Satoh N."/>
                <person name="Rokhsar D.S."/>
            </authorList>
            <dbReference type="PubMed" id="12481130"/>
            <dbReference type="DOI" id="10.1126/science.1080049"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2008" name="Genome Biol." volume="9" first="R152" last="R152">
            <title>Improved genome assembly and evidence-based global gene model set for the chordate Ciona intestinalis: new insight into intron and operon populations.</title>
            <authorList>
                <person name="Satou Y."/>
                <person name="Mineta K."/>
                <person name="Ogasawara M."/>
                <person name="Sasakura Y."/>
                <person name="Shoguchi E."/>
                <person name="Ueno K."/>
                <person name="Yamada L."/>
                <person name="Matsumoto J."/>
                <person name="Wasserscheid J."/>
                <person name="Dewar K."/>
                <person name="Wiley G.B."/>
                <person name="Macmil S.L."/>
                <person name="Roe B.A."/>
                <person name="Zeller R.W."/>
                <person name="Hastings K.E."/>
                <person name="Lemaire P."/>
                <person name="Lindquist E."/>
                <person name="Endo T."/>
                <person name="Hotta K."/>
                <person name="Inaba K."/>
            </authorList>
            <dbReference type="PubMed" id="18854010"/>
            <dbReference type="DOI" id="10.1186/gb-2008-9-10-r152"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">wild type</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="4">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7 10 11">Belongs to the peptidase C12 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="7 11"/>
  